MS004349 (gene) Bitter gourd (TR) v1

Overview


Name	MS004349
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	thioredoxin-like protein YLS8
Location	scaffold92: 2079493 .. 2079732 (+)
RNA-Seq Expression	MS004349
Synteny	MS004349

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGAGTGTTGGCCTCAGTTGCCGAGACGATTAAGAACTTTGCAGTAATATATCTTGTCGATATCATGGAGGTTCCTGATTTCAACGCAATGTACGAGCTGTATGATCCTTCCACTGTCATGTTCTTCTTTAGGAACAAGCACATAATGAGATTGATCTTTGGTGCTGGAAACAACGATAAGATAAACTGGGCACTCAAGGATAAGCAGGAGTTCATTGATATCATTGAAGACGTG

mRNA sequence

ATGGATGGAGTGTTGGCCTCAGTTGCCGAGACGATTAAGAACTTTGCAGTAATATATCTTGTCGATATCATGGAGGTTCCTGATTTCAACGCAATGTACGAGCTGTATGATCCTTCCACTGTCATGTTCTTCTTTAGGAACAAGCACATAATGAGATTGATCTTTGGTGCTGGAAACAACGATAAGATAAACTGGGCACTCAAGGATAAGCAGGAGTTCATTGATATCATTGAAGACGTG

Coding sequence (CDS)

ATGGATGGAGTGTTGGCCTCAGTTGCCGAGACGATTAAGAACTTTGCAGTAATATATCTTGTCGATATCATGGAGGTTCCTGATTTCAACGCAATGTACGAGCTGTATGATCCTTCCACTGTCATGTTCTTCTTTAGGAACAAGCACATAATGAGATTGATCTTTGGTGCTGGAAACAACGATAAGATAAACTGGGCACTCAAGGATAAGCAGGAGTTCATTGATATCATTGAAGACGTG

Protein sequence

MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNNDKINWALKDKQEFIDIIEDV

Homology

[Close]

BLAST of MS004349 vs. NCBI nr
Match: XP_022139325.1 (thioredoxin-like protein YLS8 [Momordica charantia])

HSP 1 Score: 158.3 bits (399), Expect = 2.8e-35
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MDGVLASVAETIKNFAVIYLVDI+EVPDFNAMYELYD STVMFFFRNKHIMRLIFGAGNN
Sbjct: 23  MDGVLASVAETIKNFAVIYLVDIIEVPDFNAMYELYDHSTVMFFFRNKHIMRLIFGAGNN 82

Query: 61  DKINWALKDKQEFIDIIEDV 81
           DKINWALKDKQEFIDIIEDV
Sbjct: 83  DKINWALKDKQEFIDIIEDV 102

[Close]

BLAST of MS004349 vs. NCBI nr
Match: MBA0750948.1 (hypothetical protein [Gossypium gossypioides])

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 76  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 135

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 136 NKINWALKDKQEFIDIIETV 154

[Close]

BLAST of MS004349 vs. NCBI nr
Match: XP_004143618.1 (thioredoxin-like protein YLS8 isoform X1 [Cucumis sativus] >XP_022139324.1 thioredoxin-like protein YLS8 isoform X2 [Momordica charantia] >XP_022999573.1 thioredoxin-like protein YLS8 [Cucurbita maxima] >XP_038892358.1 thioredoxin-like protein YLS8 isoform X2 [Benincasa hispida])

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. NCBI nr
Match: XP_040967487.1 (thioredoxin-like protein YLS8 [Gossypium hirsutum] >KAG4205497.1 hypothetical protein ERO13_A04G108600v2 [Gossypium hirsutum])

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 106 MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 165

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 166 NKINWALKDKQEFIDIIETV 184

[Close]

BLAST of MS004349 vs. NCBI nr
Match: NP_196446.1 (mRNA splicing factor, thioredoxin-like U5 snRNP [Arabidopsis thaliana] >Q9FE62.1 RecName: Full=Thioredoxin-like protein YLS8; AltName: Full=Protein YELLOW-LEAF-SPECIFIC GENE 8 [Arabidopsis thaliana] >KAG7601599.1 Thioredoxin-like superfamily [Arabidopsis thaliana x Arabidopsis arenosa] >KAG7608536.1 Thioredoxin-like superfamily [Arabidopsis suecica] >AAG40036.1 AT5g08290 [Arabidopsis thaliana] >AAG41439.1 unknown protein [Arabidopsis thaliana] >AAK00362.1 unknown protein [Arabidopsis thaliana])

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy Swiss-Prot
Match: Q9FE62 (Thioredoxin-like protein YLS8 OS=Arabidopsis thaliana OX=3702 GN=YLS8 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 5.1e-32
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy Swiss-Prot
Match: P83876 (Thioredoxin-like protein 4A OS=Homo sapiens OX=9606 GN=TXNL4A PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 3.4e-28
Identity = 61/80 (76.25%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VL S+AE +KNFAVIYLVDI EVPDFN MYELYDP TVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLYSIAEKVKNFAVIYLVDITEVPDFNKMYELYDPCTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWA++DKQE +DIIE V
Sbjct: 101 NKINWAMEDKQEMVDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy Swiss-Prot
Match: P83877 (Thioredoxin-like protein 4A OS=Mus musculus OX=10090 GN=Txnl4a PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 3.4e-28
Identity = 61/80 (76.25%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VL S+AE +KNFAVIYLVDI EVPDFN MYELYDP TVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLYSIAEKVKNFAVIYLVDITEVPDFNKMYELYDPCTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWA++DKQE +DIIE V
Sbjct: 101 NKINWAMEDKQEMVDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy Swiss-Prot
Match: Q553S5 (Thioredoxin-like protein 4A homolog OS=Dictyostelium discoideum OX=44689 GN=txnl4a PE=3 SV=4)

HSP 1 Score: 109.8 bits (273), Expect = 1.5e-23
Identity = 53/79 (67.09%), Postives = 63/79 (79.75%), Query Frame = 0


Query: 2   DGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNND 61
           D +LAS+AE +KN AVIY+VDI EVPD N+MYELYD    MFF+RNKHIM +  G GNN+
Sbjct: 33  DDILASIAEKVKNMAVIYVVDITEVPDLNSMYELYDDCPTMFFYRNKHIM-VDLGTGNNN 92

Query: 62  KINWALKDKQEFIDIIEDV 81
           KINWAL +KQ+ IDIIE V
Sbjct: 93  KINWALTNKQDMIDIIETV 110

[Close]

BLAST of MS004349 vs. ExPASy Swiss-Prot
Match: P87215 (Mitosis protein dim1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dim1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.9e-23
Identity = 53/79 (67.09%), Postives = 63/79 (79.75%), Query Frame = 0


Query: 2   DGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNND 61
           D VL  +AE + N AVIYLVDI EVPDFN MYELYD +T+MFF+RNKH+M +  G GNN+
Sbjct: 42  DEVLYRIAEKVVNMAVIYLVDIDEVPDFNKMYELYDRTTIMFFYRNKHMM-IDLGTGNNN 101

Query: 62  KINWALKDKQEFIDIIEDV 81
           KINW L+DKQE IDIIE +
Sbjct: 102 KINWPLEDKQEMIDIIETI 119

[Close]

BLAST of MS004349 vs. ExPASy TrEMBL
Match: A0A6J1CC08 (thioredoxin-like protein YLS8 OS=Momordica charantia OX=3673 GN=LOC111010266 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-35
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MDGVLASVAETIKNFAVIYLVDI+EVPDFNAMYELYD STVMFFFRNKHIMRLIFGAGNN
Sbjct: 23  MDGVLASVAETIKNFAVIYLVDIIEVPDFNAMYELYDHSTVMFFFRNKHIMRLIFGAGNN 82

Query: 61  DKINWALKDKQEFIDIIEDV 81
           DKINWALKDKQEFIDIIEDV
Sbjct: 83  DKINWALKDKQEFIDIIEDV 102

[Close]

BLAST of MS004349 vs. ExPASy TrEMBL
Match: S4U0X2 (YLS8 OS=Gossypium hirsutum OX=3635 GN=LOC107944993 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy TrEMBL
Match: A0A7I8L052 (Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI7747_10013014 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy TrEMBL
Match: A0A6J1I6E9 (thioredoxin-like protein YLS8 OS=Cucurbita maxima OX=3661 GN=LOC111470351 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. ExPASy TrEMBL
Match: A0A6P4D2R7 (thioredoxin-like protein YLS8 isoform X1 OS=Arachis duranensis OX=130453 GN=LOC107485414 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-29
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

[Close]

BLAST of MS004349 vs. TAIR 10
Match: AT5G08290.1 (mRNA splicing factor, thioredoxin-like U5 snRNP )

HSP 1 Score: 137.9 bits (346), Expect = 3.6e-33
Identity = 70/80 (87.50%), Postives = 72/80 (90.00%), Query Frame = 0


Query: 1   MDGVLASVAETIKNFAVIYLVDIMEVPDFNAMYELYDPSTVMFFFRNKHIMRLIFGAGNN 60
           MD VLASVAETIKNFAVIYLVDI EVPDFN MYELYDPSTVMFFFRNKHIM +  G GNN
Sbjct: 41  MDEVLASVAETIKNFAVIYLVDITEVPDFNTMYELYDPSTVMFFFRNKHIM-IDLGTGNN 100

Query: 61  DKINWALKDKQEFIDIIEDV 81
           +KINWALKDKQEFIDIIE V
Sbjct: 101 NKINWALKDKQEFIDIIETV 119

The following BLAST results are available for this feature:

BLAST of MS004349 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139325.1	2.8e-35	97.50	thioredoxin-like protein YLS8 [Momordica charantia]	[more]
MBA0750948.1	3.9e-29	87.50	hypothetical protein [Gossypium gossypioides]	[more]
XP_004143618.1	3.9e-29	87.50	thioredoxin-like protein YLS8 isoform X1 [Cucumis sativus] >XP_022139324.1 thior...	[more]
XP_040967487.1	3.9e-29	87.50	thioredoxin-like protein YLS8 [Gossypium hirsutum] >KAG4205497.1 hypothetical pr...	[more]
NP_196446.1	3.9e-29	87.50	mRNA splicing factor, thioredoxin-like U5 snRNP [Arabidopsis thaliana] >Q9FE62.1...	[more]

BLAST of MS004349 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FE62	5.1e-32	87.50	Thioredoxin-like protein YLS8 OS=Arabidopsis thaliana OX=3702 GN=YLS8 PE=1 SV=1	[more]
P83876	3.4e-28	76.25	Thioredoxin-like protein 4A OS=Homo sapiens OX=9606 GN=TXNL4A PE=1 SV=1	[more]
P83877	3.4e-28	76.25	Thioredoxin-like protein 4A OS=Mus musculus OX=10090 GN=Txnl4a PE=1 SV=1	[more]
Q553S5	1.5e-23	67.09	Thioredoxin-like protein 4A homolog OS=Dictyostelium discoideum OX=44689 GN=txnl...	[more]
P87215	1.9e-23	67.09	Mitosis protein dim1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=2...	[more]

BLAST of MS004349 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC08	1.3e-35	97.50	thioredoxin-like protein YLS8 OS=Momordica charantia OX=3673 GN=LOC111010266 PE=...	[more]
S4U0X2	1.9e-29	87.50	YLS8 OS=Gossypium hirsutum OX=3635 GN=LOC107944993 PE=2 SV=1	[more]
A0A7I8L052	1.9e-29	87.50	Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI7747_10013014 PE=3 SV...	[more]
A0A6J1I6E9	1.9e-29	87.50	thioredoxin-like protein YLS8 OS=Cucurbita maxima OX=3661 GN=LOC111470351 PE=3 S...	[more]
A0A6P4D2R7	1.9e-29	87.50	thioredoxin-like protein YLS8 isoform X1 OS=Arachis duranensis OX=130453 GN=LOC1...	[more]

BLAST of MS004349 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08290.1	3.6e-33	87.50	mRNA splicing factor, thioredoxin-like U5 snRNP	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004123	Dim1 family	SMART	SM01410	DIM1_2	coord: 1..80 e-value: 4.7E-19 score: 79.3
IPR004123	Dim1 family	PFAM	PF02966	DIM1	coord: 1..80 e-value: 2.4E-31 score: 108.3
IPR004123	Dim1 family	PANTHER	PTHR12052	THIOREDOXIN-LIKE PROTEN 4A, 4B	coord: 1..80
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..80 e-value: 1.4E-34 score: 120.6
None	No IPR available	PANTHER	PTHR12052:SF7	THIOREDOXIN-LIKE PROTEIN YLS8 ISOFORM X1	coord: 1..80
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 1..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004349.1	MS004349.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0046540	U4/U6 x U5 tri-snRNP complex