MS004302 (gene) Bitter gourd (TR) v1

Overview
NameMS004302
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein MIZU-KUSSEI 1
Locationscaffold92: 1657971 .. 1658684 (-)
RNA-Seq ExpressionMS004302
SyntenyMS004302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCCGAGAAAATCTCCGGCGCCGTCGCCGCCGCGGCCAGCGATTTCGCTCCAACCTTCACCAAAGCGATCAGGTCTCTCAAAGCCTACGAACAAACTCCTCCGGCAAGTCCGCTCCGTGTTCCGCACGCTTCCGATTCTGTCTCCGGCGTGCCGATTTCCTTTAAGCATGAATCGGCTCCACGACGGCCACGGCCACGGCCACGGCGGAGCGCGGATCACCGGAACGATCTTCGGGTACCGGAAATCGCGAGTGAATCTGGCGTTTCAGGAAAGCCCTAGGTGTCTCCCGATGCTGATTCTGGAACTCGCGATTCCGACGGGGAAAATCCTGCAGGATTTGGGGCTAGGGCTAGGGCTCGTCCGGCTGGCCCTGGAGTGCGAGAAGCGGCCGGCGGATCAGAAGAGGAAGATTCTGGAGGAGCCGATTTGGACATTGTACTGCAACGGAAGAAAATCCGGCTACGGCGTCCGCAGAGATCCGACGGAGGACGATCTCAAAATTATGCAGACGCTCTCCGCTGTGTCGATGGGCGCCGGCGTGATTCCGGCCGACGAAGCGGCGGCCGCGGCAGCTGACGGCGATCAATTGACGTATATGAGAGCGCATTTCGAGCGAGTGACTGGATCCAAGGATTCCGAGACGTTCTACATGATGAATCCTGACGCCAACAATGGCGCTGAACTCAGTATCTTTATGGTTAGAATT

mRNA sequence

ATGGCGGAGCCGAGAAAATCTCCGGCGCCGTCGCCGCCGCGGCCAGCGATTTCGCTCCAACCTTCACCAAAGCGATCAGGTCTCTCAAAGCCTACGAACAAACTCCTCCGGCAAGTCCGCTCCGTGTTCCGCACGCTTCCGATTCTGTCTCCGGCGTGCCGATTTCCTTTAAGCATGAATCGGCTCCACGACGGCCACGGCCACGGCCACGGCGGAGCGCGGATCACCGGAACGATCTTCGGGTACCGGAAATCGCGAGTGAATCTGGCGTTTCAGGAAAGCCCTAGGTGTCTCCCGATGCTGATTCTGGAACTCGCGATTCCGACGGGGAAAATCCTGCAGGATTTGGGGCTAGGGCTAGGGCTCGTCCGGCTGGCCCTGGAGTGCGAGAAGCGGCCGGCGGATCAGAAGAGGAAGATTCTGGAGGAGCCGATTTGGACATTGTACTGCAACGGAAGAAAATCCGGCTACGGCGTCCGCAGAGATCCGACGGAGGACGATCTCAAAATTATGCAGACGCTCTCCGCTGTGTCGATGGGCGCCGGCGTGATTCCGGCCGACGAAGCGGCGGCCGCGGCAGCTGACGGCGATCAATTGACGTATATGAGAGCGCATTTCGAGCGAGTGACTGGATCCAAGGATTCCGAGACGTTCTACATGATGAATCCTGACGCCAACAATGGCGCTGAACTCAGTATCTTTATGGTTAGAATT

Coding sequence (CDS)

ATGGCGGAGCCGAGAAAATCTCCGGCGCCGTCGCCGCCGCGGCCAGCGATTTCGCTCCAACCTTCACCAAAGCGATCAGGTCTCTCAAAGCCTACGAACAAACTCCTCCGGCAAGTCCGCTCCGTGTTCCGCACGCTTCCGATTCTGTCTCCGGCGTGCCGATTTCCTTTAAGCATGAATCGGCTCCACGACGGCCACGGCCACGGCCACGGCGGAGCGCGGATCACCGGAACGATCTTCGGGTACCGGAAATCGCGAGTGAATCTGGCGTTTCAGGAAAGCCCTAGGTGTCTCCCGATGCTGATTCTGGAACTCGCGATTCCGACGGGGAAAATCCTGCAGGATTTGGGGCTAGGGCTAGGGCTCGTCCGGCTGGCCCTGGAGTGCGAGAAGCGGCCGGCGGATCAGAAGAGGAAGATTCTGGAGGAGCCGATTTGGACATTGTACTGCAACGGAAGAAAATCCGGCTACGGCGTCCGCAGAGATCCGACGGAGGACGATCTCAAAATTATGCAGACGCTCTCCGCTGTGTCGATGGGCGCCGGCGTGATTCCGGCCGACGAAGCGGCGGCCGCGGCAGCTGACGGCGATCAATTGACGTATATGAGAGCGCATTTCGAGCGAGTGACTGGATCCAAGGATTCCGAGACGTTCTACATGATGAATCCTGACGCCAACAATGGCGCTGAACTCAGTATCTTTATGGTTAGAATT

Protein sequence

MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI
Homology
BLAST of MS004302 vs. NCBI nr
Match: XP_022139011.1 (protein MIZU-KUSSEI 1 [Momordica charantia])

HSP 1 Score: 469.5 bits (1207), Expect = 1.7e-128
Identity = 236/238 (99.16%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN 60
           MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN
Sbjct: 1   MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN 60

Query: 61  RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL 120
           RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL
Sbjct: 61  RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL 120

Query: 121 GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG 180
           GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG
Sbjct: 121 GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG 180

Query: 181 AGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 239
           AGVIPADE AAAAADG QLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI
Sbjct: 181 AGVIPADEVAAAAADGYQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 238

BLAST of MS004302 vs. NCBI nr
Match: KAG6601106.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031906.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.7 bits (979), Expect = 4.6e-102
Identity = 202/247 (81.78%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 1   MAEPRKSPAPSP---------PRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+P         P PA+SLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPLRSSPQSSPLPAMSLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNLAFQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLAFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYGVRRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGVRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD+NNGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAQTADGDQLTYMRAYFERVTGSKDSETFYMMNPDSNNGAEL 240

BLAST of MS004302 vs. NCBI nr
Match: XP_023511741.1 (protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 381.7 bits (979), Expect = 4.6e-102
Identity = 201/247 (81.38%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 1   MAEPRKSPAPSPPR---------PAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+PP+         P ISLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPQRSSPQSSPLPEISLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNLAFQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLAFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYG+RRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGIRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD+NNGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAETADGDQLTYMRAYFERVTGSKDSETFYMMNPDSNNGAEL 240

BLAST of MS004302 vs. NCBI nr
Match: XP_022986387.1 (protein MIZU-KUSSEI 1 [Cucurbita maxima])

HSP 1 Score: 381.3 bits (978), Expect = 6.0e-102
Identity = 202/247 (81.78%), Postives = 221/247 (89.47%), Query Frame = 0

Query: 1   MAEPRKSPAPSP---------PRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+P         P PAISLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPLRSSPQSSPLPAISLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNL FQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLVFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYGVRRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGVRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD+NNGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAETADGDQLTYMRAYFERVTGSKDSETFYMMNPDSNNGAEL 240

BLAST of MS004302 vs. NCBI nr
Match: XP_022957187.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata])

HSP 1 Score: 379.8 bits (974), Expect = 1.7e-101
Identity = 201/247 (81.38%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 1   MAEPRKSPAPSP---------PRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+P         P PA+SLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPLRSSPQSSPLPAMSLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNLAFQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLAFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYGVRRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGVRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD++NGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAETADGDQLTYMRAYFERVTGSKDSETFYMMNPDSSNGAEL 240

BLAST of MS004302 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.1e-37
Identity = 81/175 (46.29%), Postives = 116/175 (66.29%), Query Frame = 0

Query: 72  GARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGLGLVRLALECEK 131
           G R+TGT++G+++  V  + Q + R  P+L+L+LA+ T  ++++  +  GLVR+ALECEK
Sbjct: 126 GRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKE--MSSGLVRIALECEK 185

Query: 132 RPADQKRKILEEPIWTLYCNGRKSGYGVRRDP--TEDDLKIMQTLSAVSMGAGVIPADE- 191
           R      K+ +EP WT+YCNGRK GY V R    T+ D +++ T+S V++GAGVIP  + 
Sbjct: 186 RHR-SGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKT 245

Query: 192 -----AAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 239
                   +  +  +L YMR  FERV GS+DSE FYMMNPD N G ELSIF++RI
Sbjct: 246 IDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of MS004302 vs. ExPASy TrEMBL
Match: A0A6J1CBD4 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111010047 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.1e-129
Identity = 236/238 (99.16%), Postives = 236/238 (99.16%), Query Frame = 0

Query: 1   MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN 60
           MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN
Sbjct: 1   MAEPRKSPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPLSMN 60

Query: 61  RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL 120
           RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL
Sbjct: 61  RLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGL 120

Query: 121 GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG 180
           GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG
Sbjct: 121 GLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSMG 180

Query: 181 AGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 239
           AGVIPADE AAAAADG QLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI
Sbjct: 181 AGVIPADEVAAAAADGYQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 238

BLAST of MS004302 vs. ExPASy TrEMBL
Match: A0A6J1J7E5 (protein MIZU-KUSSEI 1 OS=Cucurbita maxima OX=3661 GN=LOC111484137 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.9e-102
Identity = 202/247 (81.78%), Postives = 221/247 (89.47%), Query Frame = 0

Query: 1   MAEPRKSPAPSP---------PRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+P         P PAISLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPLRSSPQSSPLPAISLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNL FQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLVFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYGVRRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGVRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD+NNGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAETADGDQLTYMRAYFERVTGSKDSETFYMMNPDSNNGAEL 240

BLAST of MS004302 vs. ExPASy TrEMBL
Match: A0A6J1GYG4 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111458655 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 8.4e-102
Identity = 201/247 (81.38%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 1   MAEPRKSPAPSP---------PRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRKSP+P+P         P PA+SLQPSPKRSG SKPT KLLRQVRSVFRTLPILSP
Sbjct: 1   MAEPRKSPSPAPPLRSSPQSSPLPAMSLQPSPKRSGFSKPT-KLLRQVRSVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACR PL+ +RLHD  G+ HGGARITGTIFGYRKSRVNLAFQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRIPLNGSRLHD--GYVHGGARITGTIFGYRKSRVNLAFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +LQD  +G GLVRLALECEKRP+ +KRKILEEPIWTLYCNGRKSGYGVRRDP+ +D+KIM
Sbjct: 121 LLQD--MGQGLVRLALECEKRPS-EKRKILEEPIWTLYCNGRKSGYGVRRDPSSEDMKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA+E AA  ADGDQLTYMRA+FERVTGSKDSETFYMMNPD++NGAEL
Sbjct: 181 QTLNAVSMGAGVIPAEE-AAETADGDQLTYMRAYFERVTGSKDSETFYMMNPDSSNGAEL 240

BLAST of MS004302 vs. ExPASy TrEMBL
Match: A0A6J1G2I4 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450060 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.5e-100
Identity = 198/247 (80.16%), Postives = 220/247 (89.07%), Query Frame = 0

Query: 1   MAEPRK--SPA-------PSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP 60
           MAEPRK  SPA       P PPRPAISLQPSPKRSG SKPT KLLRQ+R+VFRTLPILSP
Sbjct: 1   MAEPRKFSSPALQSSPSPPPPPRPAISLQPSPKRSGFSKPT-KLLRQIRTVFRTLPILSP 60

Query: 61  ACRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 120
           ACRFP++ +RLHD  GH HGG RITGTIFGYRKSRVNL FQESPRCLP+LILELAIPTGK
Sbjct: 61  ACRFPINGSRLHD--GHVHGGDRITGTIFGYRKSRVNLVFQESPRCLPILILELAIPTGK 120

Query: 121 ILQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIM 180
           +L+D  +G+GLVRLALECEKRP+ +KRKILEEPIWTLYCNG+KSGYGVRRDP+E+DLKIM
Sbjct: 121 LLRD--MGVGLVRLALECEKRPS-EKRKILEEPIWTLYCNGKKSGYGVRRDPSEEDLKIM 180

Query: 181 QTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAEL 239
           QTL+AVSMGAGVIPA++ AA  ADGDQLTYMR  FERVTGSKDSETFYMMNP++NNG EL
Sbjct: 181 QTLNAVSMGAGVIPAED-AAETADGDQLTYMRVDFERVTGSKDSETFYMMNPESNNGGEL 240

BLAST of MS004302 vs. ExPASy TrEMBL
Match: A0A6J1HTQ9 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466511 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.0e-99
Identity = 197/246 (80.08%), Postives = 219/246 (89.02%), Query Frame = 0

Query: 1   MAEPRK--------SPAPSPPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPA 60
           MAEPRK        SP+P PPRPAISLQPSPKRSG SKPT KLLRQ+R+VFRTLPILSPA
Sbjct: 1   MAEPRKFSSPAIQSSPSP-PPRPAISLQPSPKRSGFSKPT-KLLRQIRTVFRTLPILSPA 60

Query: 61  CRFPLSMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKI 120
           CRFPL+ +RLHD  GH HGG RITGTIFGYRKSRVNL FQESPRCLP+LILELAIPTGK+
Sbjct: 61  CRFPLNGSRLHD--GHVHGGDRITGTIFGYRKSRVNLVFQESPRCLPILILELAIPTGKL 120

Query: 121 LQDLGLGLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQ 180
           L+D  +GLGLVRLALECEKRP+ +KRKILEEPIWTLYCNG+KSGYGVRRDP+E+DLKIMQ
Sbjct: 121 LRD--MGLGLVRLALECEKRPS-EKRKILEEPIWTLYCNGKKSGYGVRRDPSEEDLKIMQ 180

Query: 181 TLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELS 239
           TL+AVSMGAGVIP+++ AA   DGDQLTYMR  FERVTGSKDSETFYMMNP++NNG ELS
Sbjct: 181 TLNAVSMGAGVIPSED-AAETVDGDQLTYMRVDFERVTGSKDSETFYMMNPESNNGGELS 238

BLAST of MS004302 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 283.1 bits (723), Expect = 2.1e-76
Identity = 148/248 (59.68%), Postives = 184/248 (74.19%), Query Frame = 0

Query: 1   MAEPRKSPAPSPPRPAISL-QPSPKRSGLSKPTNKLLRQVRSVFRTLPILSPACRFPL-S 60
           +  P+   + +  RP I+L QPS +       + KL R+VRSVFR+LPI+SP C+FP+  
Sbjct: 19  LGSPKSKKSTATVRPEITLEQPSGRNKTTGSKSTKLFRRVRSVFRSLPIMSPMCKFPVGG 78

Query: 61  MNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGL 120
             RLH+   H HGG R+TGT+FGYRK+RVNLA QE+PR LP+L+LELAIPTGK+LQD  L
Sbjct: 79  GGRLHE--NHVHGGTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQD--L 138

Query: 121 GLGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVS 180
           G+GLVR+ALECEK+P+ +K KI++EPIW LYCNG+KSGYGV+R PTE+DL +MQ L AVS
Sbjct: 139 GVGLVRIALECEKKPS-EKTKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVS 198

Query: 181 MGAGVIPADEAAAAAADG--------DQLTYMRAHFERVTGSKDSETFYMMNPDANNGAE 239
           MGAGV+P    A     G          LTYMRAHFERV GS+DSET+YMMNPD N+G E
Sbjct: 199 MGAGVLPVSSGAITEQSGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPE 258

BLAST of MS004302 vs. TAIR 10
Match: AT2G21990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 229.6 bits (584), Expect = 2.7e-60
Identity = 125/239 (52.30%), Postives = 170/239 (71.13%), Query Frame = 0

Query: 4   PRKSPAPSP-PRPAIS-LQPSPKRSGLSKPTNKLLRQVRSVFRTLPILSP-ACRFP-LSM 63
           P  SP+PSP PR  ++ L+PS +     K + K+ R  RSVFR+ PI++P AC+ P L  
Sbjct: 21  PSASPSPSPAPRQHVTLLEPSHQH---KKKSKKVFRVFRSVFRSFPIITPAACKIPVLPG 80

Query: 64  NRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLG 123
             L D H  G  G+R+TGT+FGYRK RV+L+ QESPRCLP L++ELA+ T  + ++  L 
Sbjct: 81  GSLPDQHRSGSSGSRVTGTLFGYRKGRVSLSIQESPRCLPSLVVELAMQTMVLQKE--LS 140

Query: 124 LGLVRLALECEKRPADQKRKILEEPIWTLYCNGRKSGYGVRRDPTEDDLKIMQTLSAVSM 183
            G+VR+ALE EKR   +K KI++EP+WT++ NG+K+GYGV+RD TE+DL +M+ L  VSM
Sbjct: 141 GGMVRIALETEKRGDKEKIKIMDEPLWTMFSNGKKTGYGVKRDATEEDLNVMELLRPVSM 200

Query: 184 GAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 239
           GAGV+P +          ++ YMRA+FERV GSKDSETFYM++P+ NNG ELSIF VR+
Sbjct: 201 GAGVLPGN--TEFEGPDSEMAYMRAYFERVVGSKDSETFYMLSPEGNNGPELSIFFVRV 252

BLAST of MS004302 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 211.8 bits (538), Expect = 5.8e-55
Identity = 122/255 (47.84%), Postives = 166/255 (65.10%), Query Frame = 0

Query: 4   PRKSPAPS------PPRPAISLQPSPKRSGLSKPTNKLLRQVRSVFRTLPIL---SPACR 63
           P  SP PS      PPR    LQP   +    K    + R +R+VFR+ PI    S AC+
Sbjct: 20  PPSSPTPSPTSTTTPPRQHPLLQPPSSKK--KKNRTNVFRVLRTVFRSFPIFTTPSVACK 79

Query: 64  FPLSMNRLHDGHG---HGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGK 123
            P+    +H G G     H  +RITGT+FGYRK RV+L+ QE+P+CLP L++ELA+ T  
Sbjct: 80  IPV----IHPGLGLPDPHHNTSRITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTT 139

Query: 124 ILQDLGLGLGLVRLALECEKRP--------ADQKRKILEEPIWTLYCNGRKSGYGVRRDP 183
           + ++  L  G+VR+ALE EK+P         ++K  ILEEP+WT+YC G K+GYGV+R+ 
Sbjct: 140 LQKE--LSTGMVRIALETEKQPRADNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREA 199

Query: 184 TEDDLKIMQTLSAVSMGAGVIPADEAAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNP 239
           TE+DL +M+ L  VSMGAGV+P + + +   DG ++ YMRA+FERV GSKDSETFYM++P
Sbjct: 200 TEEDLNVMELLRPVSMGAGVLPGN-SESEGPDG-EMAYMRAYFERVIGSKDSETFYMLSP 259

BLAST of MS004302 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 158.3 bits (399), Expect = 7.6e-39
Identity = 81/175 (46.29%), Postives = 116/175 (66.29%), Query Frame = 0

Query: 72  GARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLGLGLGLVRLALECEK 131
           G R+TGT++G+++  V  + Q + R  P+L+L+LA+ T  ++++  +  GLVR+ALECEK
Sbjct: 126 GRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKE--MSSGLVRIALECEK 185

Query: 132 RPADQKRKILEEPIWTLYCNGRKSGYGVRRDP--TEDDLKIMQTLSAVSMGAGVIPADE- 191
           R      K+ +EP WT+YCNGRK GY V R    T+ D +++ T+S V++GAGVIP  + 
Sbjct: 186 RHR-SGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKT 245

Query: 192 -----AAAAAADGDQLTYMRAHFERVTGSKDSETFYMMNPDANNGAELSIFMVRI 239
                   +  +  +L YMR  FERV GS+DSE FYMMNPD N G ELSIF++RI
Sbjct: 246 IDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of MS004302 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 157.1 bits (396), Expect = 1.7e-38
Identity = 78/207 (37.68%), Postives = 122/207 (58.94%), Query Frame = 0

Query: 58  SMNRLHDGHGHGHGGARITGTIFGYRKSRVNLAFQESPRCLPMLILELAIPTGKILQDLG 117
           +++R   G+ +   G+R+ GT+FG R+  V+ + Q+ P   P  ++ELA P   ++++  
Sbjct: 73  TLSRARPGNQNSGLGSRVVGTLFGSRRGHVHFSIQKDPNSPPAFLIELATPISGLVKE-- 132

Query: 118 LGLGLVRLALECEKRPADQK-------------------------RKILEEPIWTLYCNG 177
           +  GLVR+ALEC+K   +++                         R+++EEP+W  YCNG
Sbjct: 133 MASGLVRIALECDKGKEEEEGEEKNGTLRHGGGDKTKTTTTAAVSRRLVEEPMWRTYCNG 192

Query: 178 RKSGYGVRRDPTEDDLKIMQTLSAVSMGAGVIP-ADEAAAAAADGDQLTYMRAHFERVTG 237
           +K G+  RR+  E + K+++ L  VSMGAGV+P  +E       G  + YMRA FER+ G
Sbjct: 193 KKCGFATRRECGEKEKKVLKALEMVSMGAGVLPETEEIGGGGGGGGDIMYMRAKFERIVG 252

Query: 238 SKDSETFYMMNPDANNGAELSIFMVRI 239
           S+DSE FYMMNPD+N   ELSI+++RI
Sbjct: 253 SRDSEAFYMMNPDSNGAPELSIYLLRI 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139011.11.7e-12899.16protein MIZU-KUSSEI 1 [Momordica charantia][more]
KAG6601106.14.6e-10281.78Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG70319... [more]
XP_023511741.14.6e-10281.38protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo][more]
XP_022986387.16.0e-10281.78protein MIZU-KUSSEI 1 [Cucurbita maxima][more]
XP_022957187.11.7e-10181.38protein MIZU-KUSSEI 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O222271.1e-3746.29Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CBD48.1e-12999.16protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111010047 PE=4 SV=1[more]
A0A6J1J7E52.9e-10281.78protein MIZU-KUSSEI 1 OS=Cucurbita maxima OX=3661 GN=LOC111484137 PE=4 SV=1[more]
A0A6J1GYG48.4e-10281.38protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111458655 PE=4 SV=1[more]
A0A6J1G2I43.5e-10080.16protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450060 PE=4 SV... [more]
A0A6J1HTQ91.0e-9980.08protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466511 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G06990.12.1e-7659.68Protein of unknown function, DUF617 [more]
AT2G21990.12.7e-6052.30Protein of unknown function, DUF617 [more]
AT4G39610.15.8e-5547.84Protein of unknown function, DUF617 [more]
AT2G41660.17.6e-3946.29Protein of unknown function, DUF617 [more]
AT5G23100.11.7e-3837.68Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 75..237
e-value: 7.9E-65
score: 217.9
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 75..238
e-value: 2.9E-66
score: 220.3
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 4..238
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availablePANTHERPTHR31696:SF73EXPRESSED PROTEINcoord: 4..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004302.1MS004302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010274 hydrotropism