MS004295 (gene) Bitter gourd (TR) v1

Overview
NameMS004295
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCilia-and flagella-associated protein 20
Locationscaffold92: 1589966 .. 1590276 (-)
RNA-Seq ExpressionMS004295
SyntenyMS004295
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCAAAAATACGTTTCAGTCCGGATTCCTCTCTATATTATATAGCCTCGGGTATGTTTAAATTTCTTAACGCTAGTACTTACAAATACAATCTGCCGAATTTGTTGTTTTTTCAATTCCAGGTACTGAGGATTTGTTTTATTTTGTGTACCCATTTTTTCTGTAGGAGCAAACCCCTTCAGATATGGGACAAAGAAGGTGAGTTTGTTCATCTGCTGACTGATGTCTTTGTTTTACATTTGCTTCACATTAAATTTTGCATGTTTGGTGTTCGAGAACGTGGTTATGGTGTTCTTGAATGGTATGAAG

mRNA sequence

ATGTTCAAAAATACGTTTCAGTCCGGATTCCTCTCTATATTATATAGCCTCGGGAGCAAACCCCTTCAGATATGGGACAAAGAAGGTGAGTTTGTTCATCTGCTGACTGATGTCTTTGTTTTACATTTGCTTCACAATTTTGCATGTTTGGTGTTCGAGAACGTGGTTATGGTGTTCTTGAATGGTATGAAG

Coding sequence (CDS)

ATGTTCAAAAATACGTTTCAGTCCGGATTCCTCTCTATATTATATAGCCTCGGGAGCAAACCCCTTCAGATATGGGACAAAGAAGGTGAGTTTGTTCATCTGCTGACTGATGTCTTTGTTTTACATTTGCTTCACAATTTTGCATGTTTGGTGTTCGAGAACGTGGTTATGGTGTTCTTGAATGGTATGAAG

Protein sequence

MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHLLTDVFVLHLLHNFACLVFENVVMVFLNGMK
Homology
BLAST of MS004295 vs. NCBI nr
Match: XP_008446984.1 (PREDICTED: cilia- and flagella-associated protein 20 isoform X1 [Cucumis melo])

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-07
Identity = 29/31 (93.55%), Postives = 31/31 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEF 32
          MFKNTFQSGFLSIL+SLGSKPLQIWDKEGE+
Sbjct: 1  MFKNTFQSGFLSILFSLGSKPLQIWDKEGEY 31

BLAST of MS004295 vs. NCBI nr
Match: KVI00746.1 (hypothetical protein Ccrd_021001, partial [Cynara cardunculus var. scolymus])

HSP 1 Score: 65.9 bits (159), Expect = 1.5e-07
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0

Query: 1   MFKNTFQSGFLSILYSLGSKPLQIWDKEGE 31
           MFKNTFQSGFLSILYSLGSKPLQIWDKEG+
Sbjct: 94  MFKNTFQSGFLSILYSLGSKPLQIWDKEGQ 123

BLAST of MS004295 vs. NCBI nr
Match: KAG2246071.1 (hypothetical protein Bca52824_085699 [Brassica carinata])

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-07
Identity = 30/34 (88.24%), Postives = 31/34 (91.18%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYSLGSKPLQIWDKE E  H+
Sbjct: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEAEDGHV 34

BLAST of MS004295 vs. NCBI nr
Match: RZB81696.1 (Cilia- and flagella-associated protein 20, partial [Glycine soja])

HSP 1 Score: 64.7 bits (156), Expect = 3.3e-07
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 30
          MFKNTFQSGFLSILYSLGSKPLQIWDKEG
Sbjct: 27 MFKNTFQSGFLSILYSLGSKPLQIWDKEG 55

BLAST of MS004295 vs. NCBI nr
Match: ONI16820.1 (hypothetical protein PRUPE_3G123900 [Prunus persica] >ONI16821.1 hypothetical protein PRUPE_3G123900 [Prunus persica] >ONI16822.1 hypothetical protein PRUPE_3G123900 [Prunus persica] >ONI16823.1 hypothetical protein PRUPE_3G123900 [Prunus persica])

HSP 1 Score: 64.7 bits (156), Expect = 3.3e-07
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 30
          MFKNTFQSGFLSILYSLGSKPLQIWDKEG
Sbjct: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 29

BLAST of MS004295 vs. ExPASy Swiss-Prot
Match: Q6B857 (Cilia- and flagella-associated protein 20 OS=Bos taurus OX=9913 GN=CFAP20 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYS+GSKPLQIWDK+    H+
Sbjct: 1  MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHI 34

BLAST of MS004295 vs. ExPASy Swiss-Prot
Match: Q6PBJ2 (Cilia- and flagella-associated protein 20 OS=Danio rerio OX=7955 GN=cfap20 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYS+GSKPLQIWDK+    H+
Sbjct: 1  MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHI 34

BLAST of MS004295 vs. ExPASy Swiss-Prot
Match: Q9Y6A4 (Cilia- and flagella-associated protein 20 OS=Homo sapiens OX=9606 GN=CFAP20 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYS+GSKPLQIWDK+    H+
Sbjct: 1  MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHI 34

BLAST of MS004295 vs. ExPASy Swiss-Prot
Match: Q8BTU1 (Cilia- and flagella-associated protein 20 OS=Mus musculus OX=10090 GN=Cfap20 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYS+GSKPLQIWDK+    H+
Sbjct: 1  MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHI 34

BLAST of MS004295 vs. ExPASy Swiss-Prot
Match: Q499T7 (Cilia- and flagella-associated protein 20 OS=Rattus norvegicus OX=10116 GN=Cfap20 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHL 35
          MFKNTFQSGFLSILYS+GSKPLQIWDK+    H+
Sbjct: 1  MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHI 34

BLAST of MS004295 vs. ExPASy TrEMBL
Match: A0A0A0KV68 (DUF667 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G626020 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.0e-14
Identity = 44/65 (67.69%), Postives = 50/65 (76.92%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEFVHLLTDVFVLHLL-HNFACLVFENVVMVF 60
          MFKNTFQSGFLSIL+SLGSKPLQIWDKEGE       VF+LHL+  +  CLVFE   + F
Sbjct: 1  MFKNTFQSGFLSILFSLGSKPLQIWDKEGECFRYRDAVFILHLIKDSLVCLVFEYTAIKF 60

Query: 61 LNGMK 65
          LNG+K
Sbjct: 61 LNGLK 65

BLAST of MS004295 vs. ExPASy TrEMBL
Match: A0A1S3BGZ6 (cilia- and flagella-associated protein 20 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489531 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.5e-08
Identity = 29/31 (93.55%), Postives = 31/31 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEGEF 32
          MFKNTFQSGFLSIL+SLGSKPLQIWDKEGE+
Sbjct: 1  MFKNTFQSGFLSILFSLGSKPLQIWDKEGEY 31

BLAST of MS004295 vs. ExPASy TrEMBL
Match: A0A103Y1D6 (Uncharacterized protein (Fragment) OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_021001 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.2e-08
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0

Query: 1   MFKNTFQSGFLSILYSLGSKPLQIWDKEGE 31
           MFKNTFQSGFLSILYSLGSKPLQIWDKEG+
Sbjct: 94  MFKNTFQSGFLSILYSLGSKPLQIWDKEGQ 123

BLAST of MS004295 vs. ExPASy TrEMBL
Match: A0A251PZ28 (DUF667 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G123900 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-07
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 30
          MFKNTFQSGFLSILYSLGSKPLQIWDKEG
Sbjct: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 29

BLAST of MS004295 vs. ExPASy TrEMBL
Match: A0A445I6L7 (Cilia-and flagella-associated protein 20 (Fragment) OS=Glycine soja OX=3848 GN=D0Y65_031104 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-07
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKEG 30
          MFKNTFQSGFLSILYSLGSKPLQIWDKEG
Sbjct: 27 MFKNTFQSGFLSILYSLGSKPLQIWDKEG 55

BLAST of MS004295 vs. TAIR 10
Match: AT3G12300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF667 (InterPro:IPR007714); Has 373 Blast hits to 371 proteins in 116 species: Archae - 0; Bacteria - 0; Metazoa - 213; Fungi - 4; Plants - 71; Viruses - 0; Other Eukaryotes - 85 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-10
Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 0

Query: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKE 29
          MFKNTFQSGFLSILYSLGSKPLQIWDKE
Sbjct: 1  MFKNTFQSGFLSILYSLGSKPLQIWDKE 28

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446984.11.1e-0793.55PREDICTED: cilia- and flagella-associated protein 20 isoform X1 [Cucumis melo][more]
KVI00746.11.5e-0796.67hypothetical protein Ccrd_021001, partial [Cynara cardunculus var. scolymus][more]
KAG2246071.12.5e-0788.24hypothetical protein Bca52824_085699 [Brassica carinata][more]
RZB81696.13.3e-07100.00Cilia- and flagella-associated protein 20, partial [Glycine soja][more]
ONI16820.13.3e-07100.00hypothetical protein PRUPE_3G123900 [Prunus persica] >ONI16821.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q6B8571.1e-0879.41Cilia- and flagella-associated protein 20 OS=Bos taurus OX=9913 GN=CFAP20 PE=2 S... [more]
Q6PBJ21.1e-0879.41Cilia- and flagella-associated protein 20 OS=Danio rerio OX=7955 GN=cfap20 PE=2 ... [more]
Q9Y6A41.1e-0879.41Cilia- and flagella-associated protein 20 OS=Homo sapiens OX=9606 GN=CFAP20 PE=1... [more]
Q8BTU11.1e-0879.41Cilia- and flagella-associated protein 20 OS=Mus musculus OX=10090 GN=Cfap20 PE=... [more]
Q499T71.1e-0879.41Cilia- and flagella-associated protein 20 OS=Rattus norvegicus OX=10116 GN=Cfap2... [more]
Match NameE-valueIdentityDescription
A0A0A0KV683.0e-1467.69DUF667 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G626020 PE=4... [more]
A0A1S3BGZ65.5e-0893.55cilia- and flagella-associated protein 20 isoform X1 OS=Cucumis melo OX=3656 GN=... [more]
A0A103Y1D67.2e-0896.67Uncharacterized protein (Fragment) OS=Cynara cardunculus var. scolymus OX=59895 ... [more]
A0A251PZ281.6e-07100.00DUF667 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G123900 PE=... [more]
A0A445I6L71.6e-07100.00Cilia-and flagella-associated protein 20 (Fragment) OS=Glycine soja OX=3848 GN=D... [more]
Match NameE-valueIdentityDescription
AT3G12300.12.0e-10100.00unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007714CFA20 domainPFAMPF05018DUF667coord: 1..29
e-value: 1.1E-8
score: 35.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004295.1MS004295.1mRNA