MS003989 (gene) Bitter gourd (TR) v1

BLAST of MS003989 vs. NCBI nr
Match: XP_022137595.1 (uncharacterized protein LOC111009000 [Momordica charantia])

HSP 1 Score: 936.8 bits (2420), Expect = 7.9e-269
Identity = 491/508 (96.65%), Postives = 493/508 (97.05%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE 60
           MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE 60

Query: 61  DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW 120
           DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW
Sbjct: 61  DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW 120

Query: 121 IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED 180
           IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED
Sbjct: 121 IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED 180

Query: 181 CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS 240
           CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS
Sbjct: 181 CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS 240

Query: 241 SKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE 300
           +KMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE
Sbjct: 241 NKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE 300

Query: 301 LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES 360
           LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES
Sbjct: 301 LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES 360

Query: 361 SESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPEFVEDFAEGELRNDMINGQT 420
           SESMSRIEAELEAELERLGLNINTQGVERFPDEDE  LDPEFVEDFAEGELRNDMINGQT
Sbjct: 361 SESMSRIEAELEAELERLGLNINTQGVERFPDEDE--LDPEFVEDFAEGELRNDMINGQT 420

Query: 421 SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKLQQL 480
           SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKL QL
Sbjct: 421 SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKL-QL 480

Query: 481 IDTEYKSSWLELGNGELQFSSHENSPNA 509
           IDTE KSSWL+L       S  EN  NA
Sbjct: 481 IDTENKSSWLDLDQ-----SGWENGLNA 500

BLAST of MS003989 vs. NCBI nr
Match: XP_023519559.1 (uncharacterized protein LOC111782939 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519560.1 uncharacterized protein LOC111782939 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519561.1 uncharacterized protein LOC111782939 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023519562.1 uncharacterized protein LOC111782939 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023519563.1 uncharacterized protein LOC111782939 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.8 bits (1636), Expect = 6.5e-178
Identity = 359/500 (71.80%), Postives = 405/500 (81.00%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFG+SNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGDSNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAEL  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAELACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+ V +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----NSEERVGESSKSNTMANDIGISVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTYE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSL  C      E KLPKGS+FDA ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLDPCIAVELEEHKLPKGSHFDADESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE S SKG  DES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHSDSKGGLDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 CLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPEFVEDFAEGEL 420
           LY+H A ESSES+ RIEAELEAELERLGLNI+T+G ER+PD+D+  L PEF EDFAEGEL
Sbjct: 361 LYKHMAEESSESIHRIEAELEAELERLGLNISTEGTERYPDDDDDELGPEFEEDFAEGEL 420

Query: 421 RNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQ 480
           RNDM++G +   +K N+EASDST HSGNYTVSPREL+LRLHDVIQSRLE+RI+ELE+ALQ
Sbjct: 421 RNDMMSGGSCGWSKANKEASDSTVHSGNYTVSPRELSLRLHDVIQSRLEARIEELENALQ 480

Query: 481 KNQRKLQQLIDTEYKSSWLE 492
            N +K QQL++TEY SSWLE
Sbjct: 481 NNHKK-QQLLNTEYTSSWLE 484

BLAST of MS003989 vs. NCBI nr
Match: XP_022923691.1 (uncharacterized protein LOC111431325 isoform X3 [Cucurbita moschata])

HSP 1 Score: 631.7 bits (1628), Expect = 5.5e-177
Identity = 356/500 (71.20%), Postives = 407/500 (81.40%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPEFVEDFAEGEL 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+PD+D+  L PEF EDFAEGEL
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDE-LGPEFEEDFAEGEL 420

Query: 421 RNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQ 480
           RNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE+RI+ELE+ALQ
Sbjct: 421 RNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLEARIEELENALQ 480

Query: 481 KNQRKLQQLIDTEYKSSWLE 492
            N +K QQL++TEY SSWLE
Sbjct: 481 NNYKK-QQLLNTEYTSSWLE 483

BLAST of MS003989 vs. NCBI nr
Match: XP_022923690.1 (uncharacterized protein LOC111431325 isoform X2 [Cucurbita moschata])

HSP 1 Score: 629.8 bits (1623), Expect = 2.1e-176
Identity = 356/502 (70.92%), Postives = 407/502 (81.08%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFP--DEDEVLLDPEFVEDFAEG 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+P  D+D+  L PEF EDFAEG
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDDDDELGPEFEEDFAEG 420

Query: 421 ELRNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESA 480
           ELRNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE+RI+ELE+A
Sbjct: 421 ELRNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLEARIEELENA 480

Query: 481 LQKNQRKLQQLIDTEYKSSWLE 492
           LQ N +K QQL++TEY SSWLE
Sbjct: 481 LQNNYKK-QQLLNTEYTSSWLE 486

BLAST of MS003989 vs. NCBI nr
Match: XP_022923688.1 (uncharacterized protein LOC111431325 isoform X1 [Cucurbita moschata] >XP_022923689.1 uncharacterized protein LOC111431325 isoform X1 [Cucurbita moschata])

HSP 1 Score: 629.4 bits (1622), Expect = 2.7e-176
Identity = 357/511 (69.86%), Postives = 408/511 (79.84%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDE-----------VLLDP 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+PD+D+           V L P
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDDDDENHRSIHSVQLGP 420

Query: 421 EFVEDFAEGELRNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLE 480
           EF EDFAEGELRNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE
Sbjct: 421 EFEEDFAEGELRNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLE 480

Query: 481 SRIKELESALQKNQRKLQQLIDTEYKSSWLE 492
           +RI+ELE+ALQ N +K QQL++TEY SSWLE
Sbjct: 481 ARIEELENALQNNYKK-QQLLNTEYTSSWLE 495

BLAST of MS003989 vs. ExPASy TrEMBL
Match: A0A6J1C733 (uncharacterized protein LOC111009000 OS=Momordica charantia OX=3673 GN=LOC111009000 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.8e-269
Identity = 491/508 (96.65%), Postives = 493/508 (97.05%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE 60
           MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE 60

Query: 61  DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW 120
           DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW
Sbjct: 61  DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDLDVLSIPNMTWKSW 120

Query: 121 IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED 180
           IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED
Sbjct: 121 IDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSVED 180

Query: 181 CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS 240
           CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS
Sbjct: 181 CLVFRESSLSLCETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSS 240

Query: 241 SKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE 300
           +KMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE
Sbjct: 241 NKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEE 300

Query: 301 LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES 360
           LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES
Sbjct: 301 LEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVES 360

Query: 361 SESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPEFVEDFAEGELRNDMINGQT 420
           SESMSRIEAELEAELERLGLNINTQGVERFPDEDE  LDPEFVEDFAEGELRNDMINGQT
Sbjct: 361 SESMSRIEAELEAELERLGLNINTQGVERFPDEDE--LDPEFVEDFAEGELRNDMINGQT 420

Query: 421 SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKLQQL 480
           SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKL QL
Sbjct: 421 SARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKL-QL 480

Query: 481 IDTEYKSSWLELGNGELQFSSHENSPNA 509
           IDTE KSSWL+L       S  EN  NA
Sbjct: 481 IDTENKSSWLDLDQ-----SGWENGLNA 500

BLAST of MS003989 vs. ExPASy TrEMBL
Match: A0A6J1E7G0 (uncharacterized protein LOC111431325 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431325 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 2.6e-177
Identity = 356/500 (71.20%), Postives = 407/500 (81.40%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPEFVEDFAEGEL 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+PD+D+  L PEF EDFAEGEL
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDE-LGPEFEEDFAEGEL 420

Query: 421 RNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESALQ 480
           RNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE+RI+ELE+ALQ
Sbjct: 421 RNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLEARIEELENALQ 480

Query: 481 KNQRKLQQLIDTEYKSSWLE 492
            N +K QQL++TEY SSWLE
Sbjct: 481 NNYKK-QQLLNTEYTSSWLE 483

BLAST of MS003989 vs. ExPASy TrEMBL
Match: A0A6J1ECL1 (uncharacterized protein LOC111431325 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431325 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 1.0e-176
Identity = 356/502 (70.92%), Postives = 407/502 (81.08%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFP--DEDEVLLDPEFVEDFAEG 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+P  D+D+  L PEF EDFAEG
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDDDDELGPEFEEDFAEG 420

Query: 421 ELRNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELESA 480
           ELRNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE+RI+ELE+A
Sbjct: 421 ELRNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLEARIEELENA 480

Query: 481 LQKNQRKLQQLIDTEYKSSWLE 492
           LQ N +K QQL++TEY SSWLE
Sbjct: 481 LQNNYKK-QQLLNTEYTSSWLE 486

BLAST of MS003989 vs. ExPASy TrEMBL
Match: A0A6J1E743 (uncharacterized protein LOC111431325 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431325 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.3e-176
Identity = 357/511 (69.86%), Postives = 408/511 (79.84%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFGESNLVSPQYSNHLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGESNLVSPQYSNHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAE+  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAEMACISGGFEIGKLGLFGSHQDFNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MANDIG+LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----TSEERVGESSKSNTMANDIGILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTCE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+F+  ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFNGDESVCAVSQLPFGPL--SDTVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PDES +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDESLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDE-----------VLLDP 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G ER+PD+D+           V L P
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTERYPDDDDDDDENHRSIHSVQLGP 420

Query: 421 EFVEDFAEGELRNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLE 480
           EF EDFAEGELRNDM++G++   +K N+EA++ST HSGNYTVSPREL+LRLHDVIQSRLE
Sbjct: 421 EFEEDFAEGELRNDMMSGESCGWSKANKEANESTVHSGNYTVSPRELSLRLHDVIQSRLE 480

Query: 481 SRIKELESALQKNQRKLQQLIDTEYKSSWLE 492
           +RI+ELE+ALQ N +K QQL++TEY SSWLE
Sbjct: 481 ARIEELENALQNNYKK-QQLLNTEYTSSWLE 495

BLAST of MS003989 vs. ExPASy TrEMBL
Match: A0A6J1KHA7 (uncharacterized protein LOC111495744 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495744 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 1.0e-173
Identity = 354/503 (70.38%), Postives = 403/503 (80.12%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGD-ALSQLSFGESNLVSPQYSNHLLDKFSRRKKRF 60
           MDLWIVAAATGAGYVAKYWKNQS DGD +LSQLSFG+SNL SPQYS HLL KFS+RKKR+
Sbjct: 1   MDLWIVAAATGAGYVAKYWKNQSKDGDCSLSQLSFGDSNLASPQYSYHLLSKFSQRKKRY 60

Query: 61  EDVFGHGRMEGTSSDHNPLDIAASVAELGSMN-GFENEKLGLWGNHQDLDVLSIPNMTWK 120
           EDVFGHG MEGTSSD NP DI ASVAEL  ++ GFE  KLGL+G+HQD +V S PNMT  
Sbjct: 61  EDVFGHGGMEGTSSDQNPSDI-ASVAELACISGGFEIGKLGLFGSHQDSNVFSTPNMTL- 120

Query: 121 SWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSSV 180
                +S+  VGESS SN MAN+I +LV +SS R  S RNRSTAK R+LHGDLIKPL+  
Sbjct: 121 -----NSEERVGESSKSNTMANNIEILVCNSSGRTASSRNRSTAKGRYLHGDLIKPLTYE 180

Query: 181 EDCLVFRESSLSLC------ETKLPKGSNFDAKESVCGVSQPPFGSLTTSDIVSN-KTVK 240
           EDCL   +SSLS C      E KLPKGS+FDA ESVC VSQ PFG L  SD VSN KT K
Sbjct: 181 EDCLPAHQSSLSPCIAVELEEHKLPKGSHFDADESVCAVSQLPFGPL--SDKVSNKKTGK 240

Query: 241 GWERNSRSSSKMVKREDSASKGAPDESFILYLGFFIGIIYSFLSSKREVYKLEELLKQTE 300
            WER SRSS+KM  RE   SKG PD+S +LYLGFFIGIIYSF+S+KREV+KL+ELLKQTE
Sbjct: 241 EWERKSRSSNKMANREHFDSKGGPDKSLVLYLGFFIGIIYSFISNKREVHKLKELLKQTE 300

Query: 301 YLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEE 360
            LV+DLQEELEMKDSL LKEL++D      T+SN FSEKT D SSPKHVMDYTVNFN EE
Sbjct: 301 SLVEDLQEELEMKDSLTLKELSND------TYSNGFSEKTVDESSPKHVMDYTVNFNAEE 360

Query: 361 LYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDE---VLLDPEFVEDFAE 420
           LY+H A +SSES+ RIEAELEAELERLGLNI+T+G E +PD+D+   V L PEF EDFA+
Sbjct: 361 LYKHMAEQSSESIHRIEAELEAELERLGLNISTEGTESYPDDDDDDYVQLGPEFEEDFAK 420

Query: 421 GELRNDMINGQTSARTKPNEEASDSTAHSGNYTVSPRELTLRLHDVIQSRLESRIKELES 480
           GELRNDM++G+    +K N+EA DST HSGNYTVSPREL+LRLHDVIQSRLE+RIKELE+
Sbjct: 421 GELRNDMLSGENCGWSKANKEACDSTVHSGNYTVSPRELSLRLHDVIQSRLEARIKELEN 480

Query: 481 ALQKNQRKLQQLIDTEYKSSWLE 492
           ALQ N +K QQL++TE KSSWLE
Sbjct: 481 ALQNNHKK-QQLLNTEDKSSWLE 487

BLAST of MS003989 vs. TAIR 10
Match: AT5G61040.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G08010.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 176.0 bits (445), Expect = 7.7e-44
Identity = 171/514 (33.27%), Postives = 250/514 (48.64%), Query Frame = 0

Query: 1   MDLWIVAAATGAGYVAKYWKNQSGDGDALSQLSFGESNLVSPQYSNHLLDKFSRRKKRFE 60
           MD+W++AA    GY+AK  +N +   D + + S  +    SP     LL +  R KK  E
Sbjct: 1   MDVWLIAATAATGYIAKQLQNVTKGKDNVLESSSEDVKPESP--PGCLLSRLVRVKKANE 60

Query: 61  DVFGHGRMEGTSSDHNPLDIAASVAELGSMNGFENEKLGLWGNHQDL---DVLSIPNMTW 120
           + FG  +M   S   NP D + S    G  +G+         NH D     +   P M  
Sbjct: 61  NKFGDEKM--LSDGDNP-DASTS----GESSGYYET------NHSDTLFGLMPEFPEMEL 120

Query: 121 KSWIDDSSKGHVGESSISNKMANDIGVLVRDSSRRIGSWRNRSTAKSRFLHGDLIKPLSS 180
            +W   +S   VG++ +++            S RR   +R             LIKPLSS
Sbjct: 121 GTW--KTSGNLVGDTQLNS------------SFRRNQRFRR------------LIKPLSS 180

Query: 181 VEDCLV--FRESSLSLCETKLPKGSNFDAK--------ESVCGVSQPPFGSLTTSDIV-- 240
           ++ CL+  F    +++ +       +  A         +    +S+    SL  S  +  
Sbjct: 181 MDSCLMSRFHREQMTIEDYMTSPFPSPHASVSRPLLVTDGTRVISKSAADSLWLSQHIVL 240

Query: 241 --SNKTVKGWERNSRSSSKMVKREDSASK--GAPDESFILYLGFFIGIIYSFLSSKREVY 300
                T+        SS + V  E S S+  G  D + +L +G  IGI+ SF++S+ EV 
Sbjct: 241 SEDKATLSCGVPGVESSIERVGNEKSKSRKHGLGDATMLLQIGISIGIMSSFMASQAEVS 300

Query: 301 KLEELLKQTEYLVQDLQEELEMKDSLKLKELTHDNCESEYTFSNAFSEKTEDGSSPKHVM 360
           K+++ LKQTE LV DL++ELEMKD+L +KE+  +                          
Sbjct: 301 KVKQELKQTENLVHDLEDELEMKDTLIVKEIDIE-------------------------- 360

Query: 361 DYTVNFNTEELYEHKAVESSESMSRIEAELEAELERLGLNINTQGVERFPDEDEVLLDPE 420
                         KA ESSES+S IEAELEAELERL +N+N+  +E     D + ++P+
Sbjct: 361 --------------KAAESSESISNIEAELEAELERLEINMNSSNIET-RLSDIIEMEPD 420

Query: 421 FVEDFAEGELRNDMINGQTSARTKPNEEAS-DSTAHSGNYTVSPRELTLRLHDVIQSRLE 480
              +FA+GELR D + G+    T+ N++ S +ST  SGNY VSPREL+LRLH VI SRLE
Sbjct: 421 CEVEFAQGELRADRVKGKRLDETESNQDPSGNSTPESGNYAVSPRELSLRLHKVINSRLE 432

Query: 481 SRIKELESALQKNQRKLQQLI--DTEYKSSWLEL 493
            RI ELE+ALQ++QRK++QL+      K SW  L
Sbjct: 481 KRIGELETALQESQRKVEQLVMESESKKKSWSRL 432

BLAST of MS003989 vs. TAIR 10
Match: AT5G08010.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G61040.1); Has 5732 Blast hits to 4319 proteins in 440 species: Archae - 66; Bacteria - 397; Metazoa - 2437; Fungi - 292; Plants - 238; Viruses - 35; Other Eukaryotes - 2267 (source: NCBI BLink). )

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-28
Identity = 107/313 (34.19%), Postives = 161/313 (51.44%), Query Frame = 0

Query: 199 GSNFDAKESVCGVSQPPFGSLTTSDIVSNKTVKGWERNSRSSSKMVKREDSASKGAPDES 258
           G +   + S CG+  P    L +S + + + V          +K V R      G+ D  
Sbjct: 165 GDSVSQQVSECGI--PQLRKLKSSLLYAKRGV--------GDAKSVSRRSDNGTGSNDPV 224

Query: 259 FILYLGFFIGIIYSFLSSKREVYKLEELLKQTEYLVQDLQEELEMKDSLKLKELTHDNCE 318
            +L +G  IGI+ SF++++ E+ K+    KQTE L ++L++++            HD   
Sbjct: 225 LVLCVGISIGIMSSFVANQTELNKVRAESKQTENLGKELEDDI------------HDG-- 284

Query: 319 SEYTFSNAFSEKTEDGSSPKHVMDYTVNFNTEELYEHKAVESSESMSRIEAELEAELERL 378
                                          E+  + K  E+SES+S+IEAELEAELERL
Sbjct: 285 -------------------------------EKQCDEKTAENSESISKIEAELEAELERL 344

Query: 379 GLNINTQGVERFPDEDEVLLDPEFVEDFAEGELRNDMINGQTSARTKPNEE-ASDSTAHS 438
            +N+ +  +E     D   L+P+F  +FA+GELR+D +  Q    T  N+E +S+ST  S
Sbjct: 345 EINMISSNIET-KLSDVFELEPDFEVEFAQGELRDDQVERQRFDETVSNQERSSNSTPES 404

Query: 439 GNYTVSPRELTLRLHDVIQSRLESRIKELESALQKNQRKLQQL-IDTEYKSSWLEL---G 498
           GNY VSPREL+LRL  VI S  E RIKELE+ALQ++QRK++QL I++E K   L      
Sbjct: 405 GNYIVSPRELSLRLLGVINSCYEKRIKELENALQESQRKVEQLVIESEEKKKPLSRIWET 421

Query: 499 NGELQFSSHENSP 507
           + E+++    N P
Sbjct: 465 HEEMKYKRGSNPP 421