MS003935 (gene) Bitter gourd (TR) v1

BLAST of MS003935 vs. NCBI nr
Match: XP_022137709.1 (flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia])

HSP 1 Score: 413.3 bits (1061), Expect = 1.2e-111
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+EVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN
Sbjct: 115 FKEVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 174

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 175 GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 234

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH
Sbjct: 235 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 294

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEIHITHYEIS
Sbjct: 295 PPSRVEDEIHITHYEIS 311

BLAST of MS003935 vs. NCBI nr
Match: XP_038895109.1 (flavonol synthase/flavanone 3-hydroxylase-like [Benincasa hispida])

HSP 1 Score: 362.5 bits (929), Expect = 2.4e-96
Identity = 171/197 (86.80%), Postives = 184/197 (93.40%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+EVLEEMF KLSKVC LLESIVN+ LGLPP  LKQYN++R+WDFMT LYYF AA + EN
Sbjct: 150 FKEVLEEMFKKLSKVCSLLESIVNESLGLPPDLLKQYNNDRSWDFMTTLYYFSAAEEGEN 209

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHEDGNCITLVIQD  GGLQVRK+G+WIPV PV+GAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 210 GLTHHEDGNCITLVIQDDTGGLQVRKDGQWIPVVPVEGAIVVNIGDVIQVLSNKKFKSAT 269

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLP FT++IGEKPKYRGF+FK+YQALRLKNKTH
Sbjct: 270 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPQFTEEIGEKPKYRGFQFKDYQALRLKNKTH 329

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEIHITHYEIS
Sbjct: 330 PPSRVEDEIHITHYEIS 346

BLAST of MS003935 vs. NCBI nr
Match: XP_004137405.2 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KAE8652505.1 hypothetical protein Csa_013919 [Cucumis sativus] >CCD28478.1 GA 7-oxidase [Cucumis sativus])

HSP 1 Score: 359.8 bits (922), Expect = 1.5e-95
Identity = 169/197 (85.79%), Postives = 183/197 (92.89%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E+LEEMF KLSKVCLLLESIVN+ LGLPP FLKQYN++R+WDFMT LYYF A  + EN
Sbjct: 132 FKELLEEMFKKLSKVCLLLESIVNESLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGEN 191

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHEDGNCITLV QD  GGLQVRK+GEWIPV PV+GAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 192 GLTHHEDGNCITLVFQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSAT 251

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLP FT++IGEKPKY+GF+FK+YQALRLKNKTH
Sbjct: 252 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPQFTEEIGEKPKYKGFQFKDYQALRLKNKTH 311

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI ITHYEIS
Sbjct: 312 PPSRVEDEIRITHYEIS 328

BLAST of MS003935 vs. NCBI nr
Match: XP_008441091.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo])

HSP 1 Score: 352.1 bits (902), Expect = 3.2e-93
Identity = 166/197 (84.26%), Postives = 181/197 (91.88%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E LEE+F KLSKVCLLLESIVN+ LGLPP FLKQYN++R+WDFMT LYYF A  + EN
Sbjct: 132 FKEQLEELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGEN 191

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHED NCITLVIQD  GGLQVRK+GEWIPV PV+GAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 192 GLTHHEDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSAT 251

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLP FT++IGEKPKY+GF+FK+Y A+RLKNKTH
Sbjct: 252 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTH 311

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI ITHYEIS
Sbjct: 312 PPSRVEDEIRITHYEIS 328

BLAST of MS003935 vs. NCBI nr
Match: XP_022949101.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 345.1 bits (884), Expect = 3.9e-91
Identity = 161/197 (81.73%), Postives = 181/197 (91.88%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E LEEMFLKL+ V L++ESI+N+CLGLPPGFLKQ+N++R+WDFMTNLYYFPAA   EN
Sbjct: 118 FKETLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGEN 177

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GL HHED NCITLVIQD AGGLQV+K+ EWIPVTPV+GAIVVN+GD+IQVLSNKKFKSAT
Sbjct: 178 GLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSAT 237

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKER+S+AFF +LHGDKWVEPLP FTK+IGEKPKYRGFEF EY ALRLKNKTH
Sbjct: 238 HRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTH 297

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI I HYEI+
Sbjct: 298 PPSRVEDEISIKHYEIN 314

BLAST of MS003935 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 62/178 (34.83%), Postives = 99/178 (55.62%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ-YNDNRTWDFMTNLYYFPAA--GD 60
           +R+ ++E  ++L K+   L  ++ + LGL  G+LK+ +  ++  +F T +  +P     D
Sbjct: 110 YRKTMKEFAVELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPD 169

Query: 61  NENGLTHHEDGNCITLVIQ-DAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKF 120
              GL  H D   I L+ Q D   GLQ+ K+GEW+ V P+  +IV+N+GD I+V++N K+
Sbjct: 170 LIKGLRAHSDAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKY 229

Query: 121 KSATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPH-FTKQIGEKPKYRGFEFKEYQAL 174
           KS  HRV+ Q    R S A F+N   D ++ P P    K+  + P Y  F F +Y  L
Sbjct: 230 KSVMHRVIAQSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYPKFVFDDYMKL 287

BLAST of MS003935 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.6e-21
Identity = 63/178 (35.39%), Postives = 99/178 (55.62%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ-YNDNRTWDFMTNLYYFPAA--GD 60
           +R+ ++E  ++L K+   L  ++ + LGL  G+LK+ +  ++  +F T +  +P     D
Sbjct: 110 YRKTMKEFAVELEKLAEKLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPD 169

Query: 61  NENGLTHHEDGNCITLVIQ-DAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKF 120
              GL  H D   I L+ Q D   GLQ+ K+GEW+ V PV  +IV+N+GD I+V++N K+
Sbjct: 170 LIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGEWMDVPPVHHSIVINLGDQIEVITNGKY 229

Query: 121 KSATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGE-KPKYRGFEFKEYQAL 174
           KS  HRV+ Q    R S A F+N   D ++ P P   ++  E  P Y  F F +Y  L
Sbjct: 230 KSIMHRVIAQSDGTRMSIASFYNPGDDAFISPAPALLEEKSEVSPTYPKFLFDDYMKL 287

BLAST of MS003935 vs. ExPASy Swiss-Prot
Match: Q08506 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida OX=4102 GN=ACO1 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.6e-21
Identity = 64/179 (35.75%), Postives = 99/179 (55.31%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ-YNDNRTWDFMTNLYYFPAA--GD 60
           +REV+ +   +L K+   L  ++ + LGL  G+LK  +  ++  +F T +  +P     D
Sbjct: 110 YREVMRDFAKRLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPD 169

Query: 61  NENGLTHHEDGNCITLVIQ-DAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKF 120
              GL  H D   I L+ Q D   GLQ+ K+G+WI V P++ +IVVN+GD ++V++N K+
Sbjct: 170 LIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKY 229

Query: 121 KSATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPK--YRGFEFKEYQAL 174
           KS  HRV+ QK   R S A F+N   D  + P P   ++  E+ K  Y  F F +Y  L
Sbjct: 230 KSVMHRVIAQKDGARMSLASFYNPGSDAVIYPAPALVEKEAEENKQVYPKFVFDDYMKL 288

BLAST of MS003935 vs. ExPASy Swiss-Prot
Match: Q9MB94 (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-20
Identity = 66/188 (35.11%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ--YNDNRTWDFMTNLYYFPAAGDN 60
           +R V++E  LKL K+   L  ++ + LGL  G+LK+  Y  N    F T +  +P   + 
Sbjct: 110 YRNVMKEFALKLEKLAEQLLDLLCENLGLEKGYLKKAFYGTNGP-TFGTKVSNYPPCPNP 169

Query: 61  E--NGLTHHED-GNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKK 120
           E   GL  H D G  I L   D   GLQ+ K+G+WI V P++ +IV+N+GD ++V++N K
Sbjct: 170 ELIKGLRAHTDAGGLILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGK 229

Query: 121 FKSATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPK--YRGFEFKEYQAL 180
           ++S  HRV+ Q    R S A F+N   D  + P P   ++  E+    Y  F F++Y  L
Sbjct: 230 YRSVEHRVIAQTDGTRMSIASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKL 289

Query: 181 RLKNKTHP 182
               K  P
Sbjct: 290 YAGLKFQP 296

BLAST of MS003935 vs. ExPASy Swiss-Prot
Match: Q8S932 (1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.8e-20
Identity = 62/178 (34.83%), Postives = 97/178 (54.49%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ-YNDNRTWDFMTNLYYFPAA--GD 60
           +R V+++   +L K+   L  ++ + LGL  G+LK+ +   +  +F T +  +P     D
Sbjct: 110 YRRVMKDFAERLEKLAEYLLDLLCENLGLEKGYLKKAFYGTKGPNFGTKVANYPPCPKAD 169

Query: 61  NENGLTHHEDGNCITLVIQ-DAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKF 120
              GL  H D   I L+ Q D   GLQ+ K+ +WI V P++ +IV+N+GD ++V++N K+
Sbjct: 170 LIKGLRAHTDAGGIILLFQDDKVSGLQLLKDDQWIDVPPMKHSIVINLGDQLEVITNGKY 229

Query: 121 KSATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFT-KQIGEKPKYRGFEFKEYQAL 174
           KS  HRVV Q    R S A F+N   D  + P P    K++ EK  Y  F F +Y  L
Sbjct: 230 KSVLHRVVAQTDGTRMSIASFYNPGNDAVIYPAPALVEKEVEEKEVYPKFVFDDYMKL 287

BLAST of MS003935 vs. ExPASy TrEMBL
Match: A0A6J1C816 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009081 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 5.7e-112
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+EVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN
Sbjct: 115 FKEVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 174

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 175 GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 234

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH
Sbjct: 235 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 294

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEIHITHYEIS
Sbjct: 295 PPSRVEDEIHITHYEIS 311

BLAST of MS003935 vs. ExPASy TrEMBL
Match: S0BE22 (GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox1 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 7.4e-96
Identity = 169/197 (85.79%), Postives = 183/197 (92.89%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E+LEEMF KLSKVCLLLESIVN+ LGLPP FLKQYN++R+WDFMT LYYF A  + EN
Sbjct: 132 FKELLEEMFKKLSKVCLLLESIVNESLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGEN 191

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHEDGNCITLV QD  GGLQVRK+GEWIPV PV+GAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 192 GLTHHEDGNCITLVFQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSAT 251

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLP FT++IGEKPKY+GF+FK+YQALRLKNKTH
Sbjct: 252 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPQFTEEIGEKPKYKGFQFKDYQALRLKNKTH 311

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI ITHYEIS
Sbjct: 312 PPSRVEDEIRITHYEIS 328

BLAST of MS003935 vs. ExPASy TrEMBL
Match: A0A1S3B2M0 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103485283 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.6e-93
Identity = 166/197 (84.26%), Postives = 181/197 (91.88%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E LEE+F KLSKVCLLLESIVN+ LGLPP FLKQYN++R+WDFMT LYYF A  + EN
Sbjct: 132 FKEQLEELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGEN 191

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GLTHHED NCITLVIQD  GGLQVRK+GEWIPV PV+GAIVVNIGDVIQVLSNKKFKSAT
Sbjct: 192 GLTHHEDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSAT 251

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKERFSYAFFHNLHGDKWVEPLP FT++IGEKPKY+GF+FK+Y A+RLKNKTH
Sbjct: 252 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTH 311

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI ITHYEIS
Sbjct: 312 PPSRVEDEIRITHYEIS 328

BLAST of MS003935 vs. ExPASy TrEMBL
Match: A0A6J1GB31 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111452556 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 161/197 (81.73%), Postives = 181/197 (91.88%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E LEEMFLKL+ V L++ESI+N+CLGLPPGFLKQ+N++R+WDFMTNLYYFPAA   EN
Sbjct: 118 FKETLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGEN 177

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GL HHED NCITLVIQD AGGLQV+K+ EWIPVTPV+GAIVVN+GD+IQVLSNKKFKSAT
Sbjct: 178 GLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSAT 237

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKER+S+AFF +LHGDKWVEPLP FTK+IGEKPKYRGFEF EY ALRLKNKTH
Sbjct: 238 HRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTH 297

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI I HYEI+
Sbjct: 298 PPSRVEDEISIKHYEIN 314

BLAST of MS003935 vs. ExPASy TrEMBL
Match: O23763 (Gibberellin 7-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111493375 PE=2 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.2e-91
Identity = 160/197 (81.22%), Postives = 180/197 (91.37%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           F+E LEEMFLKLS V L++ESI+N CLGLPPGFLKQ+N++R+WDFMTNLYY+PAA   EN
Sbjct: 118 FKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGEN 177

Query: 61  GLTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSAT 120
           GL HHED NCITLVIQD AGGLQV+K+ EWIPVTPV+GAIVVN+GD+IQVLSNKKFKSAT
Sbjct: 178 GLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSAT 237

Query: 121 HRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTH 180
           HRVVRQKGKER+S+AFF +LHGDKWVEPLP FTK+IGEKPKY+GFEF EY ALRLKNKTH
Sbjct: 238 HRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTH 297

Query: 181 PPSRVEDEIHITHYEIS 198
           PPSRVEDEI I HYEI+
Sbjct: 298 PPSRVEDEISIKHYEIN 314

BLAST of MS003935 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-22
Identity = 66/198 (33.33%), Postives = 108/198 (54.55%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAG-DNE 60
           FREV +E   ++ K+   L  +V+  LGLP   L  + + +T  F+   +Y P    +  
Sbjct: 158 FREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQT-SFLRFNHYPPCPNPELA 217

Query: 61  NGLTHHEDGNCITLVIQDAAGGLQV--RKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFK 120
            G+  H+DG  +T++ QD+ GGLQV  R +G+WIPV P+  A+++N+G+ IQV +N ++ 
Sbjct: 218 LGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYW 277

Query: 121 SATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKN 180
           SA HRVV    KERFS  FF     +  +EPL     +    P Y+ + + ++   R  N
Sbjct: 278 SAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISE-ENPPCYKKYNWGKFFVSR--N 337

Query: 181 KTHPPSRVEDEIHITHYE 196
           ++       + I I H++
Sbjct: 338 RSDFKKLEVENIQIDHFK 351

BLAST of MS003935 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.6e-21
Identity = 61/176 (34.66%), Postives = 90/176 (51.14%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNEN 60
           FRE  E       K+   L  +++  LGLP      Y   +   F  N Y      D   
Sbjct: 153 FREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLAL 212

Query: 61  GLTHHEDGNCITLVIQDAAGGLQV--RKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKS 120
           G+ HH+D + I+L+ QD  GGLQV  R +G W P+ PV  A+V+NIG+ +++ +N K+ S
Sbjct: 213 GVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWS 272

Query: 121 ATHRVVRQKGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALR 175
           A HRVV    +ER+S  FF     D  V+PL          PKY+G+++ ++   R
Sbjct: 273 AEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSP-ENPPKYKGYKWGKFYVSR 327

BLAST of MS003935 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.8e-21
Identity = 64/175 (36.57%), Postives = 98/175 (56.00%), Query Frame = 0

Query: 3   EVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNENGL 62
           E++ E   ++ K+  L+  ++++ LGL P  LK+ +  +    M + YY P     E GL
Sbjct: 173 EIMLEYAKRVMKLGELIFELLSEALGLNPNHLKEMDCTKGL-LMLSHYYPPCP---EPGL 232

Query: 63  TH----HEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKS 122
           T     H D + +T+++QD  GGLQVR+NG W+ V PV GA++VN+GD++Q+++N +F S
Sbjct: 233 TFGTSPHSDRSFLTILLQDHIGGLQVRQNGYWVDVPPVPGALLVNLGDLLQLMTNDQFVS 292

Query: 123 ATHRVVRQKG-KERFSYA--FFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEY 171
             HRV+  KG K R S A  F H L   +   P+     +    PKYR     EY
Sbjct: 293 VEHRVLANKGEKPRISVASFFVHPLPSLRVYGPIKELLSE-QNLPKYRDTTVTEY 342

BLAST of MS003935 vs. TAIR 10
Match: AT2G30830.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.8e-21
Identity = 56/165 (33.94%), Postives = 95/165 (57.58%), Query Frame = 0

Query: 2   REVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNENG 61
           RE++ E   ++ K+  L+  ++++ LGL P  LK+ N  ++   +++ Y      D   G
Sbjct: 168 REIMLEYSKRMMKLGELIFELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFG 227

Query: 62  LTHHEDGNCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSATH 121
           ++ H D + IT+++QD  GGLQV  +G WI V P   A++VN+GD++Q+++N KF S  H
Sbjct: 228 ISSHTDISFITILLQDHIGGLQVLHDGYWIDVPPNPEALIVNLGDLLQLITNDKFVSVEH 287

Query: 122 RVVRQKGKE-RFSYA--FFHNLHGDKWVEPLPHFTKQIGEKPKYR 164
           RV+  +G+E R S A  F H +  ++   P+     +    PKYR
Sbjct: 288 RVLANRGEEPRISSASFFMHTIPNEQVYGPMKELLSK-QNPPKYR 331

BLAST of MS003935 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 64/181 (35.36%), Postives = 100/181 (55.25%), Query Frame = 0

Query: 1   FREVLEEMFLKLSKVCLLLESIVNDCLGLPPGFLKQ-YNDNRTWDFMTNLYYFPAA--GD 60
           +R ++++   K+ K+   L  ++ + LGL  G+LK+ +  ++   F T +  +P     D
Sbjct: 110 YRTLMKDFAGKIEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPD 169

Query: 61  NENGLTHHEDGNCITLVIQ-DAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKF 120
              GL  H D   I L+ Q D   GLQ+ K+GEW+ V PV+ +IVVN+GD ++V++N K+
Sbjct: 170 LVKGLRAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKY 229

Query: 121 KSATHRVVRQ-KGKERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPK---YRGFEFKEYQA 174
           KS  HRV+ Q  G+ R S A F+N   D  + P P    +  EK K   Y  F F++Y  
Sbjct: 230 KSVEHRVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMK 289