MS003934 (gene) Bitter gourd (TR) v1

BLAST of MS003934 vs. NCBI nr
Match: XP_022137076.1 (1-aminocyclopropane-1-carboxylate oxidase-like [Momordica charantia])

HSP 1 Score: 650.2 bits (1676), Expect = 9.3e-183
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV
Sbjct: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF
Sbjct: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG
Sbjct: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSAS 243
           LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ VLSNDRCRSAS
Sbjct: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ-VLSNDRCRSAS 240

Query: 244 HRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKTH 303
           HRVVRQA KERHSYAFFYNIGGDKWVEPLAEFTREI+EPPKYRGFIYKDYLQLRMRNKTH
Sbjct: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300

Query: 304 PPSKPQDVINITYYSTST 322
           PPSKPQDVINITYYSTST
Sbjct: 301 PPSKPQDVINITYYSTST 317

BLAST of MS003934 vs. NCBI nr
Match: XP_022923754.1 (1-aminocyclopropane-1-carboxylate oxidase 1-like [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 8.8e-149
Identity = 259/319 (81.19%), Postives = 286/319 (89.66%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           M N+ IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFF-SEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSA 243
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+Q VLSNDR +SA
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQ-VLSNDRYKSA 240

Query: 244 SHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKT 303
           SHRVVRQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+E PKYRGF+YKDYLQLR+RNKT
Sbjct: 241 SHRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKT 300

Query: 304 HPPSKPQDVINITYYSTST 322
           HPPS+PQD+INI+YYSTST
Sbjct: 301 HPPSRPQDIINISYYSTST 317

BLAST of MS003934 vs. NCBI nr
Match: KAG6584452.1 (NAP1-related protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 535.0 bits (1377), Expect = 4.4e-148
Identity = 257/314 (81.85%), Postives = 284/314 (90.45%), Query Frame = 0

Query: 9   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 68
           IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  SKEFF
Sbjct: 259 IPTADLSPFF-SEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGRSKEFF 318

Query: 69  GYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSEVVK 128
           GYPL+RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDSTFNVFP+NPQ FS V+K
Sbjct: 319 GYPLDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTFNVFPSNPQGFSCVLK 378

Query: 129 ELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVGLREHK 188
           ELFS FVKTASIVESIINDCLGLPPNFL+EYN+DRKWDLMS FRYP A+E+EN+G+REHK
Sbjct: 379 ELFSSFVKTASIVESIINDCLGLPPNFLSEYNSDRKWDLMSTFRYPCASEVENIGVREHK 438

Query: 189 DVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSASHRVV 248
           DVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+Q VLSNDR +SASHRVV
Sbjct: 439 DVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQ-VLSNDRYKSASHRVV 498

Query: 249 RQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKTHPPSK 308
           RQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+E PKYRGF+YKDYLQLR+RNKTHPPS+
Sbjct: 499 RQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKTHPPSR 558

Query: 309 PQDVINITYYSTST 322
           PQD+INI+YYSTST
Sbjct: 559 PQDIINISYYSTST 570

BLAST of MS003934 vs. NCBI nr
Match: XP_023001093.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 534.3 bits (1375), Expect = 7.4e-148
Identity = 257/319 (80.56%), Postives = 284/319 (89.03%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           M NS IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNSGIPTADLSPFF-TEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGG 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYP +RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDSTFNVFP+NPQ F
Sbjct: 61  SKEFFGYPSDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTFNVFPSNPQGF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           S V+KELFS FV TASIVESIINDCLGLPPNFL EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVNTASIVESIINDCLGLPPNFLGEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSA 243
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTL+VNVGDV+Q VLSNDR +SA
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKADVDQWIPITPDRTTLIVNVGDVVQ-VLSNDRYKSA 240

Query: 244 SHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKT 303
            HRVVRQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+EPPKYRGF+Y+DYLQLR+RNKT
Sbjct: 241 GHRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEPPKYRGFLYQDYLQLRLRNKT 300

Query: 304 HPPSKPQDVINITYYSTST 322
           HPPS+PQD+INI+YYSTST
Sbjct: 301 HPPSRPQDIINISYYSTST 317

BLAST of MS003934 vs. NCBI nr
Match: KAG7020043.1 (1-aminocyclopropane-1-carboxylate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 257/319 (80.56%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           M N+ IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFF-SEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  S DKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSSDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYN+DRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNSDRKWDLMSTFRYPCASEVENIG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSA 243
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+Q VLSNDR +SA
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQ-VLSNDRYKSA 240

Query: 244 SHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKT 303
           SHRVVRQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+E PKYRGF+YKDYLQLR+RNKT
Sbjct: 241 SHRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKT 300

Query: 304 HPPSKPQDVINITYYSTST 322
           HPPS+PQD+INI+YYSTST
Sbjct: 301 HPPSRPQDIINISYYSTST 317

BLAST of MS003934 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.7e-33
Identity = 95/290 (32.76%), Postives = 149/290 (51.38%), Query Frame = 0

Query: 26  DGRKKASEI--IGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGYPLERKLEASP--L 85
           DG+++A  +  I   C E+GFFQ+VNHG+PVEL+++   VS E +    ER   + P  L
Sbjct: 12  DGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKLREERFEGSKPVQL 71

Query: 86  PGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSEVVKELFSYFVKTASIV 145
               +  G G++ +    ++ F++        N +P+NP  F E +KE      K A  +
Sbjct: 72  LDTLVKEGDGQRLDNVDWEDVFVL-----QDDNEWPSNPPDFEETMKEYREEIRKLAEKM 131

Query: 146 ESIINDCLGLPPNFLTE-YNNDRKWDLM---SAFRYPPATELENV-GLREHKDVNCITFV 205
             ++++ LG     + + ++ D +           YPP   L+ V GLR H D   +  +
Sbjct: 132 MEVMDENLGFEKGCIKKAFSGDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHTDAGGVILL 191

Query: 206 FQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSASHRVVRQAGKERH 265
           FQ D+VGGL++ +DGRW+ + P    +V+N GD I+ VLSN R +SA HRV+  +   R 
Sbjct: 192 FQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIE-VLSNGRYKSAWHRVLATSHGNRR 251

Query: 266 SYAFFYNIGGDKWVEPLA-EFTREIEEPPKYRGFIYKDYLQLRMRNKTHP 305
           S A FYN      + P A   T E   P  Y  F++ DY+ +  + K  P
Sbjct: 252 SIASFYNPSLKATIAPAAGAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 295

BLAST of MS003934 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-33
Identity = 99/305 (32.46%), Postives = 146/305 (47.87%), Query Frame = 0

Query: 1   EVKMENSRIPTADLSPFLRKEDEDEDGRKKASEI--IGKACLEYGFFQVVNHGVPVELMK 60
           EV+     IP  DL         D  G  +A  I     AC   GFFQ+ NHGVP E +K
Sbjct: 34  EVQTSGDSIPLIDL--------HDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIK 93

Query: 61  KALLVSKEFFGYPLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTF 120
           K +  ++EFF      +++   A       L   +    E+  +  +FL +  +P +   
Sbjct: 94  KMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFI 153

Query: 121 NVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRY 180
           N +P+ P SF EV  E  +        +   I++ LGL  + ++     +    M+   Y
Sbjct: 154 NEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSN-TIGKHGQHMAINYY 213

Query: 181 P--PATELENVGLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ 240
           P  P  EL   GL  HKD N IT + QDEV GL++ +DG+W+ + P P T +VN+GD +Q
Sbjct: 214 PRCPQPEL-TYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQ 273

Query: 241 VVLSNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPK-YRGFIY 296
           V+ SN++ +S  HR V  +  ER S   FY    D  + P  E   E E+ P  YR F Y
Sbjct: 274 VI-SNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTY 327

BLAST of MS003934 vs. ExPASy Swiss-Prot
Match: O04395 (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.1e-32
Identity = 91/306 (29.74%), Postives = 156/306 (50.98%), Query Frame = 0

Query: 8   RIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEF 67
           ++P  DLS        DE+     +  + KA  ++G FQVVNHG+P EL+++   V +EF
Sbjct: 1   QVPVVDLS------CPDEE---LVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREF 60

Query: 68  FGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNE------FLMMFPPDS-TFNVFPTNP 127
           F  P   K   +   G+    GYGR+ E    K +      +L  +PP S  +  +P +P
Sbjct: 61  FELPEAEKRSCAREAGS--VEGYGRRIELDIKKRKGIVDQIYLSTWPPSSVNYRYWPKSP 120

Query: 128 QSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELE 187
             + EV +E   +    +  +   +++ LGL    + E N    W +M+   YPP    +
Sbjct: 121 PDYREVNEEYARHVKTLSEKIMEWLSEGLGLGREAIKEVNG--CWYVMNINHYPPYPHSD 180

Query: 188 NV-GLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSNDRC 247
           +  GL  H D+N +T +  +E+ GL++ +D  W+ +   P+ ++VN+GD I ++LSN + 
Sbjct: 181 SFNGLEPHTDINGLTLIITNEIPGLQVFKDDHWIEVEYIPSAIIVNIGDQI-MMLSNGKY 240

Query: 248 RSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLR-- 303
           ++  H+      K R S+    +   D  V PL E T E ++PPK++   YKDY+  +  
Sbjct: 241 KNVLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSE-DDPPKFKPIAYKDYVHNKIT 291

BLAST of MS003934 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.0e-31
Identity = 92/299 (30.77%), Postives = 140/299 (46.82%), Query Frame = 0

Query: 1   EVKMENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKA 60
           EV+     IP  DL       D     R    + +  AC  YGFFQ+ NHGVP   + K 
Sbjct: 36  EVESSGDSIPLIDL------RDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKM 95

Query: 61  LLVSKEFFGYPLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNV 120
             V++EFF  P   +++   A P     L   +    ++  +  +FL +  FP +     
Sbjct: 96  QTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDFIEE 155

Query: 121 FPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPP 180
           +P++P SF EV  E  +        +   I++ LGL  + ++         +   + YPP
Sbjct: 156 WPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNY-YPP 215

Query: 181 ATELE-NVGLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVL 240
             E E   GL  HKD   IT + QD+V GL++ +D +WV ++P P T +VN+GD +QV+ 
Sbjct: 216 CPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVI- 275

Query: 241 SNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDY 294
           SND+ +S  HR V     ER S   FY    D  + P  E   E +    YR + + +Y
Sbjct: 276 SNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEY 326

BLAST of MS003934 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-30
Identity = 99/308 (32.14%), Postives = 149/308 (48.38%), Query Frame = 0

Query: 9   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 68
           IP  D S    +E      R+K    I +AC E+GFFQ+VNHG+P+EL+ K   +S + +
Sbjct: 3   IPVIDFSKLNGEE------REKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCY 62

Query: 69  GYPLERKLEAS---PLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSE 128
               E   + S    L    +    G + E    ++ F ++   D   N +P+N     E
Sbjct: 63  KTEREEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWPSN---IKE 122

Query: 129 VVKELFSYFVKTASIVESIINDCLGLPPNFLTE-YNNDRKWDLMSAF------RYPPATE 188
            + E      K AS +  ++++ LGLP  ++ + +N   +    +AF       YPP   
Sbjct: 123 TMGEYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPH 182

Query: 189 LENV-GLREHKDVNCITFVFQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSN 248
            E V GLR H D   +  +FQ DE  GL++ +DG W+ + P P  +V+N GD I+ VLSN
Sbjct: 183 PELVNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIE-VLSN 242

Query: 249 DRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQL 305
            R +SA HRV+ +    R S A FYN      + P A    E  E  KY  F++ DY+ +
Sbjct: 243 GRYKSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSE-KKYPKFVFGDYMDV 296

BLAST of MS003934 vs. ExPASy TrEMBL
Match: A0A6J1C5F5 (1-aminocyclopropane-1-carboxylate oxidase-like OS=Momordica charantia OX=3673 GN=LOC111008638 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.5e-183
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV
Sbjct: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF
Sbjct: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG
Sbjct: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSAS 243
           LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ VLSNDRCRSAS
Sbjct: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ-VLSNDRCRSAS 240

Query: 244 HRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKTH 303
           HRVVRQA KERHSYAFFYNIGGDKWVEPLAEFTREI+EPPKYRGFIYKDYLQLRMRNKTH
Sbjct: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300

Query: 304 PPSKPQDVINITYYSTST 322
           PPSKPQDVINITYYSTST
Sbjct: 301 PPSKPQDVINITYYSTST 317

BLAST of MS003934 vs. ExPASy TrEMBL
Match: A0A6J1E7A1 (1-aminocyclopropane-1-carboxylate oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431366 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.2e-149
Identity = 259/319 (81.19%), Postives = 286/319 (89.66%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           M N+ IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFF-SEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSA 243
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+Q VLSNDR +SA
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQ-VLSNDRYKSA 240

Query: 244 SHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKT 303
           SHRVVRQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+E PKYRGF+YKDYLQLR+RNKT
Sbjct: 241 SHRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKT 300

Query: 304 HPPSKPQDVINITYYSTST 322
           HPPS+PQD+INI+YYSTST
Sbjct: 301 HPPSRPQDIINISYYSTST 317

BLAST of MS003934 vs. ExPASy TrEMBL
Match: A0A6J1KPI6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111495340 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.6e-148
Identity = 257/319 (80.56%), Postives = 284/319 (89.03%), Query Frame = 0

Query: 4   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 63
           M NS IPTADLSPF   E+ DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNSGIPTADLSPFF-TEEGDEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGG 60

Query: 64  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 123
           SKEFFGYP +RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDSTFNVFP+NPQ F
Sbjct: 61  SKEFFGYPSDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTFNVFPSNPQGF 120

Query: 124 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 183
           S V+KELFS FV TASIVESIINDCLGLPPNFL EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVNTASIVESIINDCLGLPPNFLGEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 184 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVVLSNDRCRSA 243
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTL+VNVGDV+Q VLSNDR +SA
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKADVDQWIPITPDRTTLIVNVGDVVQ-VLSNDRYKSA 240

Query: 244 SHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQLRMRNKT 303
            HRVVRQAG+ERHS+AFFYNIGGDKWVEPL  F+ +I+EPPKYRGF+Y+DYLQLR+RNKT
Sbjct: 241 GHRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEPPKYRGFLYQDYLQLRLRNKT 300

Query: 304 HPPSKPQDVINITYYSTST 322
           HPPS+PQD+INI+YYSTST
Sbjct: 301 HPPSRPQDIINISYYSTST 317

BLAST of MS003934 vs. ExPASy TrEMBL
Match: S0BE26 (GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox2 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.0e-139
Identity = 241/331 (72.81%), Postives = 283/331 (85.50%), Query Frame = 0

Query: 2   VKMENSRIPTADLSPFLRK--------EDEDEDGRKK---ASEIIGKACLEYGFFQVVNH 61
           V  E   IPTADLSPFL++        +D+D+D +KK   A EIIGKAC E+GFFQVVNH
Sbjct: 2   VNKEEITIPTADLSPFLKELDQGSYSYDDDDDDQKKKKAAAIEIIGKACSEFGFFQVVNH 61

Query: 62  GVPVELMKKALLVSKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPD 121
           GVP+ LM+KALL+S +FFGYPL+RKL+ASPLPGAP+PAGYGRQP+ SPDKNEF MMFPP 
Sbjct: 62  GVPLHLMQKALLLSNQFFGYPLDRKLQASPLPGAPMPAGYGRQPDHSPDKNEFFMMFPPH 121

Query: 122 STFNVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSA 181
           STFNVFP++PQ F EVV+ELFS FVKTAS++E+IIN+CLGLPPNFL+EYNNDRKWDLMS 
Sbjct: 122 STFNVFPSHPQGFREVVEELFSCFVKTASVIENIINECLGLPPNFLSEYNNDRKWDLMST 181

Query: 182 FRYPPATELENVGLREHKDVNCITFVFQDEVGGLELK-RDGRWVPITPDPTTLVVNVGDV 241
           FRYP A+E+ENVGLREHKDVN IT +FQDEVGGLE+K  D +W+PI P+  TLV+NVGDV
Sbjct: 182 FRYPNASEIENVGLREHKDVNFITLLFQDEVGGLEVKTEDHQWIPIIPNQNTLVINVGDV 241

Query: 242 IQVVLSNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFI 301
           IQ VLSNDR +SASHRVVRQ G+ERHSYAFFYNIGGDK V+PL  FT  I++PP Y+ FI
Sbjct: 242 IQ-VLSNDRYKSASHRVVRQEGRERHSYAFFYNIGGDKLVQPLPHFTTHIDQPPNYKSFI 301

Query: 302 YKDYLQLRMRNKTHPPSKPQDVINITYYSTS 321
           YK+YLQLR+RNKTHPPS PQD+INI+YYST+
Sbjct: 302 YKEYLQLRLRNKTHPPSNPQDIINISYYSTT 331

BLAST of MS003934 vs. ExPASy TrEMBL
Match: A0A1S3B2U8 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103485123 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.3e-137
Identity = 239/326 (73.31%), Postives = 282/326 (86.50%), Query Frame = 0

Query: 9   IPTADLSPFLRK----------EDEDEDGRKK---ASEIIGKACLEYGFFQVVNHGVPVE 68
           IPTADLSPFL++          +DED+  +KK   A EIIGKAC E+GFFQVVNHGVP+ 
Sbjct: 9   IPTADLSPFLKELEESAYSYDADDEDDQKKKKKKDAIEIIGKACSEFGFFQVVNHGVPLH 68

Query: 69  LMKKALLVSKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNV 128
           LM+KALL+S +FFGYPL+RKL+ASPL GAPLPAGYGRQP++SPDKNEF MMFPP STFNV
Sbjct: 69  LMQKALLLSNQFFGYPLDRKLQASPLTGAPLPAGYGRQPDRSPDKNEFFMMFPPHSTFNV 128

Query: 129 FPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPP 188
           FP++PQ F EVV+ELFS FVKTASI+E+IIN+CLGLPPNFL+EYNNDRKWDLMS FRYP 
Sbjct: 129 FPSDPQGFREVVEELFSCFVKTASIIENIINECLGLPPNFLSEYNNDRKWDLMSTFRYPN 188

Query: 189 ATELENVGLREHKDVNCITFVFQDEVGGLELK-RDGRWVPITPDPTTLVVNVGDVIQVVL 248
           A+E+ENVGLREHKDVN IT +FQDEVGGLE+K  D +W+PI P+  +LV+NVGDVIQ VL
Sbjct: 189 ASEIENVGLREHKDVNFITLLFQDEVGGLEVKTEDHQWIPIIPNQNSLVINVGDVIQ-VL 248

Query: 249 SNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYL 308
           SNDR +SASHRVVRQAG+ERHSYAFFYNIGGDK V+PL  FT  I++PPKY+ FIYK+YL
Sbjct: 249 SNDRYKSASHRVVRQAGRERHSYAFFYNIGGDKLVQPLPHFTTHIDQPPKYKSFIYKEYL 308

Query: 309 QLRMRNKTHPPSKPQDVINITYYSTS 321
           QLR+RNKTHPP+ P+D+INI+YYST+
Sbjct: 309 QLRLRNKTHPPTNPKDIINISYYSTT 333

BLAST of MS003934 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.8e-36
Identity = 105/314 (33.44%), Postives = 148/314 (47.13%), Query Frame = 0

Query: 6   NSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSK 65
           +  IPT DLS     ED   D    A E I +AC  +GFFQV+NHG+P  L  +    + 
Sbjct: 29  SDEIPTIDLSSL---EDTHHDKTAIAKE-IAEACKRWGFFQVINHGLPSALRHRVEKTAA 88

Query: 66  EFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTF------------ 125
           EFF    E K +       P+        +   D  E    F  DST             
Sbjct: 89  EFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTEL 148

Query: 126 ----NVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMS 185
               N +P NP  F EV +E      K A  +  +++  LGLP + LT + N++    + 
Sbjct: 149 RKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQT-SFLR 208

Query: 186 AFRYPPATELE-NVGLREHKDVNCITFVFQDEVGGLELKR--DGRWVPITPDPTTLVVNV 245
              YPP    E  +G+  HKD   +T + QD VGGL++ R  DG+W+P+ P    L++N+
Sbjct: 209 FNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINM 268

Query: 246 GDVIQVVLSNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYR 301
           G+ IQ V +ND   SA HRVV    KER S  FF+    +  +EPL E   E E PP Y+
Sbjct: 269 GNCIQ-VWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISE-ENPPCYK 328

BLAST of MS003934 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.8e-36
Identity = 105/314 (33.44%), Postives = 157/314 (50.00%), Query Frame = 0

Query: 9   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 68
           IP  DLS    + D+ ED +   SE IG AC ++GFFQV+NHGVP +  ++     K FF
Sbjct: 28  IPVIDLS----RLDDPEDVQNVISE-IGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFF 87

Query: 69  GYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMF------------PPDS----T 128
             P+E K++       P+    G   +   D  E   ++            P D      
Sbjct: 88  DLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRLV 147

Query: 129 FNVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAF- 188
           +N +P +P  F E  +    +  K A  +  +I+  LGLP     +Y  ++    MS F 
Sbjct: 148 YNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQ----MSFFR 207

Query: 189 --RYPPATELE-NVGLREHKDVNCITFVFQDEVGGLELKR--DGRWVPITPDPTTLVVNV 248
             RYPP    +  +G+  HKD + I+ + QD+VGGL++ R  DG W PI P P  LV+N+
Sbjct: 208 INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINI 267

Query: 249 GDVIQVVLSNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYR 301
           G+ ++ + +ND+  SA HRVV    +ER+S  FF     D  V+PL E     E PPKY+
Sbjct: 268 GNCME-IWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSP-ENPPKYK 327

BLAST of MS003934 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.5e-34
Identity = 99/305 (32.46%), Postives = 146/305 (47.87%), Query Frame = 0

Query: 1   EVKMENSRIPTADLSPFLRKEDEDEDGRKKASEI--IGKACLEYGFFQVVNHGVPVELMK 60
           EV+     IP  DL         D  G  +A  I     AC   GFFQ+ NHGVP E +K
Sbjct: 34  EVQTSGDSIPLIDL--------HDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIK 93

Query: 61  KALLVSKEFFGYPLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTF 120
           K +  ++EFF      +++   A       L   +    E+  +  +FL +  +P +   
Sbjct: 94  KMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFI 153

Query: 121 NVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRY 180
           N +P+ P SF EV  E  +        +   I++ LGL  + ++     +    M+   Y
Sbjct: 154 NEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSN-TIGKHGQHMAINYY 213

Query: 181 P--PATELENVGLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQ 240
           P  P  EL   GL  HKD N IT + QDEV GL++ +DG+W+ + P P T +VN+GD +Q
Sbjct: 214 PRCPQPEL-TYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQ 273

Query: 241 VVLSNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPK-YRGFIY 296
           V+ SN++ +S  HR V  +  ER S   FY    D  + P  E   E E+ P  YR F Y
Sbjct: 274 VI-SNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTY 327

BLAST of MS003934 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.4e-32
Identity = 92/299 (30.77%), Postives = 140/299 (46.82%), Query Frame = 0

Query: 1   EVKMENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKA 60
           EV+     IP  DL       D     R    + +  AC  YGFFQ+ NHGVP   + K 
Sbjct: 36  EVESSGDSIPLIDL------RDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKM 95

Query: 61  LLVSKEFFGYPLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNV 120
             V++EFF  P   +++   A P     L   +    ++  +  +FL +  FP +     
Sbjct: 96  QTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDFIEE 155

Query: 121 FPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPP 180
           +P++P SF EV  E  +        +   I++ LGL  + ++         +   + YPP
Sbjct: 156 WPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNY-YPP 215

Query: 181 ATELE-NVGLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVL 240
             E E   GL  HKD   IT + QD+V GL++ +D +WV ++P P T +VN+GD +QV+ 
Sbjct: 216 CPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVI- 275

Query: 241 SNDRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDY 294
           SND+ +S  HR V     ER S   FY    D  + P  E   E +    YR + + +Y
Sbjct: 276 SNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEY 326

BLAST of MS003934 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 7.2e-32
Identity = 99/308 (32.14%), Postives = 149/308 (48.38%), Query Frame = 0

Query: 9   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 68
           IP  D S    +E      R+K    I +AC E+GFFQ+VNHG+P+EL+ K   +S + +
Sbjct: 3   IPVIDFSKLNGEE------REKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCY 62

Query: 69  GYPLERKLEAS---PLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSE 128
               E   + S    L    +    G + E    ++ F ++   D   N +P+N     E
Sbjct: 63  KTEREEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWPSN---IKE 122

Query: 129 VVKELFSYFVKTASIVESIINDCLGLPPNFLTE-YNNDRKWDLMSAF------RYPPATE 188
            + E      K AS +  ++++ LGLP  ++ + +N   +    +AF       YPP   
Sbjct: 123 TMGEYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPH 182

Query: 189 LENV-GLREHKDVNCITFVFQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVVLSN 248
            E V GLR H D   +  +FQ DE  GL++ +DG W+ + P P  +V+N GD I+ VLSN
Sbjct: 183 PELVNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIE-VLSN 242

Query: 249 DRCRSASHRVVRQAGKERHSYAFFYNIGGDKWVEPLAEFTREIEEPPKYRGFIYKDYLQL 305
            R +SA HRV+ +    R S A FYN      + P A    E  E  KY  F++ DY+ +
Sbjct: 243 GRYKSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSE-KKYPKFVFGDYMDV 296