MS003884 (gene) Bitter gourd (TR) v1

Overview
NameMS003884
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionInositol oxygenase
Locationscaffold127: 1257697 .. 1259177 (-)
RNA-Seq ExpressionMS003884
SyntenyMS003884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGTTTGTTTTGATTGCAGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGACGGAATTGGTGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGAGAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGGTACAATTCATTTACACAAAACAGAGTGCCCTCTGTTTTCCTCTGTTTTTTCTTTTCTTAACGATTTTGCTCTGTTTTCAGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGTAAGAATTAAGAACCAACAACAACAAATTTCTGGGATTCTTGAGTTTTTTTTTGGCCCGTTAATTGAATGGGGTGATTTGGTTTTGCAGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGGTAGAATTGAGAATTGGGATTGGTTTTTAAGTATGGGAGAATTTGAATCGCCAAATTGGGAATTTCAGCTTACAAAAAGTTTTGATTTTGGATTATGATTTCAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGCGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTATGAAATATAATCTTTTTTGTACCCTTTTTTTTTTTTTTGGATAAAAGCTCTGTTTTTTAACATTAATCTCTGTGGTAATTTTTGCAGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCGTAAGTGAAAAAAAAAAGAGAATTTATAATTTTGAGTTTTGGGTATAGAATAGAATCTTAAACAGAAAAAAAAATATATTTTTGGCAGCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAAGTAATTGATGATTATTTTGTTAATTAAAATTACTATTATTGTGCAAAATAATTGGATTAACTCACTGTTTGTTTATGAAATTTCAGCAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGGTAAATCCTGTGCCTTTTATTTAT

mRNA sequence

GCGTTTGTTTTGATTGCAGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGACGGAATTGGTGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGAGAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGGTACAATTCATTTACACAAAACAGAGTGCCCTCTGTTTTCCTCTGTTTTTTCTTTTCTTAACGATTTTGCTCTGTTTTCAGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGCGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGGTAAATCCTGTGCCTTTTATTTAT

Coding sequence (CDS)

GCGTTTGTTTTGATTGCAGAAGTGGAAGAGAAGAAGGGGGCGATTTCAGAGATGACGGAATTGGTGTTGGACGGAGGATTCGTGATACCCGATAATCAAACGGCGGCGGCGGCGGCGGAAGGTTTCGTGGCGCCGGAAATTAATTCGTTCGGCCAAAGTTTCAGAGACTACGCGGCGGAGAGCGAGAGGCAGAAGAGCGTAGAGGAATTCTACCGAATGAACCACATTCACCAGACGTACGATTTCGTGAAGAAAATGAGGGAACAATACGGCAAAATGGATCGCGTGGAAATGAGCATATGGGAATGCTGTGAGCTCCTAAACGACGTCGTTGATGACAGCGATCCTGATTTGGACGAACCCCAGATTCAGCATTTGCTGCAAACCGCCGAAGCCATTCGAAAAGATTACCCAGATCAAGATTGGCTGCATTTGACTGCTCTCATTCATGGTACAATTCATTTACACAAAACAGAGTGCCCTCTGTTTTCCTCTGTTTTTTCTTTTCTTAACGATTTTGCTCTGTTTTCAGATCTTGGAAAAGTGCTTCTTCTGCCAAGTTTTGGAGGGCTTCCCCAATGGGCTGTTGTGGGGGATACACACCCTCTTGGCTGCGCTTTTGACGAATCCATTGTTCACCACAAGTATTTCAAGGAAAATTCTGACTCCGAGAATCCAGCTTATAATACCAAAAATGGCATTTACTCTGAAGGCTGCGGACTAGATAACGTTATGATATCGTGGGGCCACGATGACTATATGTATTTGGTAGCTAAAGAAAATGGGTCGACTTTGCCTTCAGCTGGATTGTTCATAATCCGTTACCATTCTTTTTATCCTTTACATAAATCGGGAGCATACAAACACCTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTTCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAAAGAATTGACGTAGAAAAAGTTAAACCATACTATATGTCCCTTATTAACAAGGTAAATCCTGTGCCTTTTATTTAT

Protein sequence

AFVLIAEVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVEKVKPYYMSLINKVNPVPFIY
Homology
BLAST of MS003884 vs. NCBI nr
Match: XP_022144825.1 (inositol oxygenase 4-like [Momordica charantia])

HSP 1 Score: 600.1 bits (1546), Expect = 1.2e-167
Identity = 298/333 (89.49%), Postives = 300/333 (90.09%), Query Frame = 0

Query: 5   IAEVEEKKGAISEMTELVLDGGFVIPDNQT-AAAAAEGFVAPEINSFGQSFRDYAAESER 64
           + EVEEKKGAISEM EL LDGGFVIPDNQT AAAAAEGFVAPEINSFGQSFRDYAAES+R
Sbjct: 10  LIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSFRDYAAESDR 69

Query: 65  QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI 124
           QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI
Sbjct: 70  QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI 129

Query: 125 QHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVL 184
           QHLLQTAEAIRKDYPDQDWLHLTALIH                           DLGKVL
Sbjct: 130 QHLLQTAEAIRKDYPDQDWLHLTALIH---------------------------DLGKVL 189

Query: 185 LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN 244
           LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN
Sbjct: 190 LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN 249

Query: 245 VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH 304
           VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH
Sbjct: 250 VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH 309

Query: 305 IFNKYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           IFNKYDLYSKSKERIDVEKVKPYYMSLINK  P
Sbjct: 310 IFNKYDLYSKSKERIDVEKVKPYYMSLINKYFP 315

BLAST of MS003884 vs. NCBI nr
Match: XP_023515618.1 (inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol oxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556.6 bits (1433), Expect = 1.5e-154
Identity = 272/330 (82.42%), Postives = 284/330 (86.06%), Query Frame = 0

Query: 7   EVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 66
           EVEEKK  I+E+ ELVLDGGFV+P+   AAAA EGFVA E+N+FG SFRDY AESERQK 
Sbjct: 11  EVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRDYEAESERQKG 70

Query: 67  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 126
           VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL
Sbjct: 71  VEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 130

Query: 127 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 186
           LQTAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLP
Sbjct: 131 LQTAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLP 190

Query: 187 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 246
           SFGGLPQWAVVGDTHPLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMI
Sbjct: 191 SFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMI 250

Query: 247 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 306
           SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFN
Sbjct: 251 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFN 310

Query: 307 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 311 KYDLYSKSKELIDVEKVKPYYVSLINKYFP 313

BLAST of MS003884 vs. NCBI nr
Match: XP_022960634.1 (inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 550.8 bits (1418), Expect = 8.1e-153
Identity = 273/330 (82.73%), Postives = 285/330 (86.36%), Query Frame = 0

Query: 7   EVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 66
           EVEEKK  I+E+ ELVLDGGFV+P N+T AAA EGFVA E+N+FG SFRDY AESERQK 
Sbjct: 11  EVEEKKVGITELNELVLDGGFVVP-NET-AAAEEGFVALEMNAFGHSFRDYEAESERQKG 70

Query: 67  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 126
           VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL
Sbjct: 71  VEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 130

Query: 127 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 186
           LQTAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLP
Sbjct: 131 LQTAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLP 190

Query: 187 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 246
           SFGGLPQWAVVGDTHPLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMI
Sbjct: 191 SFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMI 250

Query: 247 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 306
           SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFN
Sbjct: 251 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFN 310

Query: 307 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 311 KYDLYSKSKELIDVEKVKPYYVSLINKYFP 311

BLAST of MS003884 vs. NCBI nr
Match: XP_022987889.1 (inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase 2-like [Cucurbita maxima])

HSP 1 Score: 545.0 bits (1403), Expect = 4.5e-151
Identity = 269/330 (81.52%), Postives = 280/330 (84.85%), Query Frame = 0

Query: 7   EVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 66
           EVEEKK  I E  ELVLDGGFV+P+     AA EGFVA E+N+FG SFRDY AESERQK 
Sbjct: 11  EVEEKKLGIIEPNELVLDGGFVVPNG--TEAAEEGFVALEMNAFGHSFRDYEAESERQKG 70

Query: 67  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 126
           VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL
Sbjct: 71  VEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 130

Query: 127 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 186
           LQTAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLP
Sbjct: 131 LQTAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLP 190

Query: 187 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 246
           SFGGLPQWAVVGDTHPLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMI
Sbjct: 191 SFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMI 250

Query: 247 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 306
           SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFN
Sbjct: 251 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFN 310

Query: 307 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSKE +DVEKVKPYY+SLINK  P
Sbjct: 311 KYDLYSKSKELVDVEKVKPYYVSLINKYFP 311

BLAST of MS003884 vs. NCBI nr
Match: XP_008450805.1 (PREDICTED: inositol oxygenase 2-like [Cucumis melo])

HSP 1 Score: 535.8 bits (1379), Expect = 2.7e-148
Identity = 261/328 (79.57%), Postives = 279/328 (85.06%), Query Frame = 0

Query: 9   EEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVE 68
           +EKK  ++E+ ELVLDGGFV+P+ +T     EGF APE+N+FG SFRDY AESERQK VE
Sbjct: 14  DEKKVGMTELKELVLDGGFVVPNART----TEGFAAPEMNAFGHSFRDYEAESERQKGVE 73

Query: 69  EFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 128
           EFYR NHIHQTY+FV KMRE+Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ
Sbjct: 74  EFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 133

Query: 129 TAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSF 188
           TAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLPSF
Sbjct: 134 TAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLPSF 193

Query: 189 GGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISW 248
           GGLPQWAVVGDTHP+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGL+NVMISW
Sbjct: 194 GGLPQWAVVGDTHPVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISW 253

Query: 249 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKY 308
           GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKY
Sbjct: 254 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKY 310

Query: 309 DLYSKSKERIDVEKVKPYYMSLINKVNP 337
           DLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 314 DLYSKSKELIDVEKVKPYYISLINKYFP 310

BLAST of MS003884 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 473.8 bits (1218), Expect = 1.7e-132
Identity = 229/336 (68.15%), Postives = 264/336 (78.57%), Query Frame = 0

Query: 2   FVLIAEVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYA-AE 61
           FV  + V+EKK       ELVLDGGFV+P ++      + F AP++N  G SFRDY   E
Sbjct: 8   FVPDSRVDEKKVIEERDNELVLDGGFVVPKSK----ETDAFDAPDMNFLGHSFRDYENGE 67

Query: 62  SERQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDE 121
           SERQ+ VEEFYRM HIHQTYDFVKKMR++YGK++++EMSIWECCELLN+VVD+SDPDLDE
Sbjct: 68  SERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESDPDLDE 127

Query: 122 PQIQHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLG 181
           PQIQHLLQTAEAIR+DYPD+DWLHLTALIH                           DLG
Sbjct: 128 PQIQHLLQTAEAIRRDYPDEDWLHLTALIH---------------------------DLG 187

Query: 182 KVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCG 241
           KVLLLP FGGLPQWAVVGDT P+GC FD + +HHKYFK N D  NP YNTKNG+Y+EGCG
Sbjct: 188 KVLLLPEFGGLPQWAVVGDTFPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCG 247

Query: 242 LDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLK 301
           LDNV++SWGHDDYMYLVAK+NG+TLP AGLFIIRYHSFYPLHK+GAY HLMN+ED  +LK
Sbjct: 248 LDNVLMSWGHDDYMYLVAKKNGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLK 307

Query: 302 WLHIFNKYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           WLH+FNKYDLYSKSK  +DVE+VKPYY+SLINK  P
Sbjct: 308 WLHVFNKYDLYSKSKVLVDVEQVKPYYISLINKYFP 312

BLAST of MS003884 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.3e-131
Identity = 230/327 (70.34%), Postives = 255/327 (77.98%), Query Frame = 0

Query: 10  EKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVEE 69
           EK G    + EL LDGGF +P   T     E F+APE+N+FG+ FRDY  ESERQK VEE
Sbjct: 17  EKSG--DNIGELKLDGGFSMPKMDT--NDDEAFLAPEMNAFGRQFRDYDVESERQKGVEE 76

Query: 70  FYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQT 129
           FYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VVD+SDPDLDEPQIQHLLQ+
Sbjct: 77  FYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQS 136

Query: 130 AEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSFG 189
           AEAIRKDYP++DWLHLTALIH                           DLGKV+ LP FG
Sbjct: 137 AEAIRKDYPNEDWLHLTALIH---------------------------DLGKVITLPQFG 196

Query: 190 GLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWG 249
           GLPQWAVVGDT P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWG
Sbjct: 197 GLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWG 256

Query: 250 HDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYD 309
           HDDYMYLVAKENGSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYD
Sbjct: 257 HDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYD 312

Query: 310 LYSKSKERIDVEKVKPYYMSLINKVNP 337
           LYSKSK  +DVEKVKPYYMSLI K  P
Sbjct: 317 LYSKSKVHVDVEKVKPYYMSLIKKYFP 312

BLAST of MS003884 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.7e-125
Identity = 222/330 (67.27%), Postives = 253/330 (76.67%), Query Frame = 0

Query: 8   VEEKKG-AISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 67
           VE+ +G    E TELVLD                GF AP  NSFG++FRDY AESER++ 
Sbjct: 19  VEKNQGNGKEEETELVLDA---------------GFEAPHTNSFGRTFRDYDAESERRRG 78

Query: 68  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 127
           VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+ +D+SDPDLDEPQI+HL
Sbjct: 79  VEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFIDESDPDLDEPQIEHL 138

Query: 128 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 187
           LQTAEAIRKDYPD+DWLHLT LIH                           DLGKVLL  
Sbjct: 139 LQTAEAIRKDYPDEDWLHLTGLIH---------------------------DLGKVLLHS 198

Query: 188 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 247
           SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++
Sbjct: 199 SFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLM 258

Query: 248 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 307
           SWGHDDYMYLVAKEN +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FN
Sbjct: 259 SWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFN 306

Query: 308 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSK R++VE+VKPYY+SL NK  P
Sbjct: 319 KYDLYSKSKVRVNVEEVKPYYLSLTNKYFP 306

BLAST of MS003884 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-123
Identity = 217/324 (66.98%), Postives = 246/324 (75.93%), Query Frame = 0

Query: 13  GAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVEEFYR 72
           G      ELVLDGGFV+PD+               N+FG +FR+Y AESER+++VEEFYR
Sbjct: 22  GGGDNAAELVLDGGFVVPDS---------------NAFGNAFRNYEAESERKETVEEFYR 81

Query: 73  MNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEA 132
           +NHI+QTYDFV++MRE+YG++D+ EM IWEC ELLN+ +DDSDPDLD PQI+HLLQTAEA
Sbjct: 82  VNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLNEFIDDSDPDLDMPQIEHLLQTAEA 141

Query: 133 IRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSFGGLP 192
           IRKD+PD+DWLHLT LIH                           DLGKVLL PSFG LP
Sbjct: 142 IRKDFPDEDWLHLTGLIH---------------------------DLGKVLLHPSFGELP 201

Query: 193 QWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDD 252
           QW+VVGDT P+GCAFDE  VH KYFKEN D  NP  NTK G YSEGCGLDNV++SWGHDD
Sbjct: 202 QWSVVGDTFPVGCAFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDD 261

Query: 253 YMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYS 312
           YMYLVAKEN +TLPSAGLFIIRYHSFYPLHK GAY HLMN+ED +NLKWL +FNKYDLYS
Sbjct: 262 YMYLVAKENKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYS 303

Query: 313 KSKERIDVEKVKPYYMSLINKVNP 337
           KS ERIDVEKVKPYYMSLI K  P
Sbjct: 322 KSNERIDVEKVKPYYMSLIEKYFP 303

BLAST of MS003884 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 9.1e-123
Identity = 212/298 (71.14%), Postives = 237/298 (79.53%), Query Frame = 0

Query: 40  EGFVAPEINSFGQSFRDYA-AESERQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEM 99
           E F+APE+N+FG+ FRDY    SERQKSVE FY   H +QT DFV+KMR +YGK+D++ M
Sbjct: 39  EVFLAPEMNAFGRQFRDYTDTNSERQKSVEHFYATQHTNQTLDFVQKMRSEYGKLDKMVM 98

Query: 100 SIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKT 159
           +IWECCEL  +VVD+SDPDLDEPQIQHLLQ+AEAIRKDYP++DWLHLTALIH        
Sbjct: 99  NIWECCELSKEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIH-------- 158

Query: 160 ECPLFSSVFSFLNDFALFSDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFK 219
                              DLGKVL LP FGGLPQWAVVGDT P+GCAFDES VHHKYF 
Sbjct: 159 -------------------DLGKVLTLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFM 218

Query: 220 ENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSF 279
           EN D  NP YNTK GIYSEGCGL+NV++SWGHDDYMYLVAKENGSTLPS GLFIIRYHSF
Sbjct: 219 ENPDFNNPKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKENGSTLPSPGLFIIRYHSF 278

Query: 280 YPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           YPLHK+GAY HLMNEED +NLKWLH+FNKYDLYSKSK  ++VEKVKPYYMSLI K  P
Sbjct: 279 YPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEKVKPYYMSLIKKYFP 309

BLAST of MS003884 vs. ExPASy TrEMBL
Match: A0A6J1CSQ8 (Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.7e-168
Identity = 298/333 (89.49%), Postives = 300/333 (90.09%), Query Frame = 0

Query: 5   IAEVEEKKGAISEMTELVLDGGFVIPDNQT-AAAAAEGFVAPEINSFGQSFRDYAAESER 64
           + EVEEKKGAISEM EL LDGGFVIPDNQT AAAAAEGFVAPEINSFGQSFRDYAAES+R
Sbjct: 10  LIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSFRDYAAESDR 69

Query: 65  QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI 124
           QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI
Sbjct: 70  QKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQI 129

Query: 125 QHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVL 184
           QHLLQTAEAIRKDYPDQDWLHLTALIH                           DLGKVL
Sbjct: 130 QHLLQTAEAIRKDYPDQDWLHLTALIH---------------------------DLGKVL 189

Query: 185 LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN 244
           LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN
Sbjct: 190 LLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDN 249

Query: 245 VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH 304
           VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH
Sbjct: 250 VMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLH 309

Query: 305 IFNKYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           IFNKYDLYSKSKERIDVEKVKPYYMSLINK  P
Sbjct: 310 IFNKYDLYSKSKERIDVEKVKPYYMSLINKYFP 315

BLAST of MS003884 vs. ExPASy TrEMBL
Match: A0A6J1HBL6 (Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.9e-153
Identity = 273/330 (82.73%), Postives = 285/330 (86.36%), Query Frame = 0

Query: 7   EVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 66
           EVEEKK  I+E+ ELVLDGGFV+P N+T AAA EGFVA E+N+FG SFRDY AESERQK 
Sbjct: 11  EVEEKKVGITELNELVLDGGFVVP-NET-AAAEEGFVALEMNAFGHSFRDYEAESERQKG 70

Query: 67  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 126
           VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL
Sbjct: 71  VEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 130

Query: 127 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 186
           LQTAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLP
Sbjct: 131 LQTAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLP 190

Query: 187 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 246
           SFGGLPQWAVVGDTHPLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMI
Sbjct: 191 SFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMI 250

Query: 247 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 306
           SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFN
Sbjct: 251 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFN 310

Query: 307 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 311 KYDLYSKSKELIDVEKVKPYYVSLINKYFP 311

BLAST of MS003884 vs. ExPASy TrEMBL
Match: A0A6J1JI24 (Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.2e-151
Identity = 269/330 (81.52%), Postives = 280/330 (84.85%), Query Frame = 0

Query: 7   EVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 66
           EVEEKK  I E  ELVLDGGFV+P+     AA EGFVA E+N+FG SFRDY AESERQK 
Sbjct: 11  EVEEKKLGIIEPNELVLDGGFVVPNG--TEAAEEGFVALEMNAFGHSFRDYEAESERQKG 70

Query: 67  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 126
           VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL
Sbjct: 71  VEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 130

Query: 127 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 186
           LQTAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLP
Sbjct: 131 LQTAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLP 190

Query: 187 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 246
           SFGGLPQWAVVGDTHPLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMI
Sbjct: 191 SFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMI 250

Query: 247 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 306
           SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFN
Sbjct: 251 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFN 310

Query: 307 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSKE +DVEKVKPYY+SLINK  P
Sbjct: 311 KYDLYSKSKELVDVEKVKPYYVSLINKYFP 311

BLAST of MS003884 vs. ExPASy TrEMBL
Match: A0A1S3BQR6 (Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.3e-148
Identity = 261/328 (79.57%), Postives = 279/328 (85.06%), Query Frame = 0

Query: 9   EEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVE 68
           +EKK  ++E+ ELVLDGGFV+P+ +T     EGF APE+N+FG SFRDY AESERQK VE
Sbjct: 14  DEKKVGMTELKELVLDGGFVVPNART----TEGFAAPEMNAFGHSFRDYEAESERQKGVE 73

Query: 69  EFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 128
           EFYR NHIHQTY+FV KMRE+Y KMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ
Sbjct: 74  EFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 133

Query: 129 TAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSF 188
           TAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLPSF
Sbjct: 134 TAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLPSF 193

Query: 189 GGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISW 248
           GGLPQWAVVGDTHP+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGL+NVMISW
Sbjct: 194 GGLPQWAVVGDTHPVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISW 253

Query: 249 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKY 308
           GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKY
Sbjct: 254 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKY 310

Query: 309 DLYSKSKERIDVEKVKPYYMSLINKVNP 337
           DLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 314 DLYSKSKELIDVEKVKPYYISLINKYFP 310

BLAST of MS003884 vs. ExPASy TrEMBL
Match: A0A0A0LZA8 (Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.2e-148
Identity = 261/328 (79.57%), Postives = 278/328 (84.76%), Query Frame = 0

Query: 9   EEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVE 68
           +EKK  I+E+ ELVLDGGFV+P+ +T     EGF APE+N+FG SFRDY AESERQK VE
Sbjct: 14  DEKKVGITELKELVLDGGFVVPNAKT-----EGFAAPEMNAFGHSFRDYEAESERQKGVE 73

Query: 69  EFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 128
           EFYR NHIHQTY+FV+KMRE+Y KMDR EMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ
Sbjct: 74  EFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQ 133

Query: 129 TAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSF 188
           TAEAIRKDYPD+DWLHLTALIH                           DLGKVLLLPSF
Sbjct: 134 TAEAIRKDYPDEDWLHLTALIH---------------------------DLGKVLLLPSF 193

Query: 189 GGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISW 248
           GGLPQWAVVGDTHP+GCAFDESIVHHKYFKEN D ENP Y+TKNGIYSEGCGLDNV ISW
Sbjct: 194 GGLPQWAVVGDTHPVGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISW 253

Query: 249 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKY 308
           GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLH++GAYKHLMNEEDAQNLKWLHIFNKY
Sbjct: 254 GHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKY 309

Query: 309 DLYSKSKERIDVEKVKPYYMSLINKVNP 337
           DLYSKSKE IDVEKVKPYY+SLINK  P
Sbjct: 314 DLYSKSKELIDVEKVKPYYISLINKYFP 309

BLAST of MS003884 vs. TAIR 10
Match: AT2G19800.1 (myo-inositol oxygenase 2 )

HSP 1 Score: 473.8 bits (1218), Expect = 1.2e-133
Identity = 229/336 (68.15%), Postives = 264/336 (78.57%), Query Frame = 0

Query: 2   FVLIAEVEEKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYA-AE 61
           FV  + V+EKK       ELVLDGGFV+P ++      + F AP++N  G SFRDY   E
Sbjct: 8   FVPDSRVDEKKVIEERDNELVLDGGFVVPKSK----ETDAFDAPDMNFLGHSFRDYENGE 67

Query: 62  SERQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDE 121
           SERQ+ VEEFYRM HIHQTYDFVKKMR++YGK++++EMSIWECCELLN+VVD+SDPDLDE
Sbjct: 68  SERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESDPDLDE 127

Query: 122 PQIQHLLQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLG 181
           PQIQHLLQTAEAIR+DYPD+DWLHLTALIH                           DLG
Sbjct: 128 PQIQHLLQTAEAIRRDYPDEDWLHLTALIH---------------------------DLG 187

Query: 182 KVLLLPSFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCG 241
           KVLLLP FGGLPQWAVVGDT P+GC FD + +HHKYFK N D  NP YNTKNG+Y+EGCG
Sbjct: 188 KVLLLPEFGGLPQWAVVGDTFPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCG 247

Query: 242 LDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLK 301
           LDNV++SWGHDDYMYLVAK+NG+TLP AGLFIIRYHSFYPLHK+GAY HLMN+ED  +LK
Sbjct: 248 LDNVLMSWGHDDYMYLVAKKNGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLK 307

Query: 302 WLHIFNKYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           WLH+FNKYDLYSKSK  +DVE+VKPYY+SLINK  P
Sbjct: 308 WLHVFNKYDLYSKSKVLVDVEQVKPYYISLINKYFP 312

BLAST of MS003884 vs. TAIR 10
Match: AT4G26260.1 (myo-inositol oxygenase 4 )

HSP 1 Score: 468.8 bits (1205), Expect = 3.8e-132
Identity = 230/327 (70.34%), Postives = 255/327 (77.98%), Query Frame = 0

Query: 10  EKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVEE 69
           EK G    + EL LDGGF +P   T     E F+APE+N+FG+ FRDY  ESERQK VEE
Sbjct: 17  EKSG--DNIGELKLDGGFSMPKMDT--NDDEAFLAPEMNAFGRQFRDYDVESERQKGVEE 76

Query: 70  FYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQT 129
           FYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VVD+SDPDLDEPQIQHLLQ+
Sbjct: 77  FYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQS 136

Query: 130 AEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSFG 189
           AEAIRKDYP++DWLHLTALIH                           DLGKV+ LP FG
Sbjct: 137 AEAIRKDYPNEDWLHLTALIH---------------------------DLGKVITLPQFG 196

Query: 190 GLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWG 249
           GLPQWAVVGDT P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWG
Sbjct: 197 GLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWG 256

Query: 250 HDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYD 309
           HDDYMYLVAKENGSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYD
Sbjct: 257 HDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYD 312

Query: 310 LYSKSKERIDVEKVKPYYMSLINKVNP 337
           LYSKSK  +DVEKVKPYYMSLI K  P
Sbjct: 317 LYSKSKVHVDVEKVKPYYMSLIKKYFP 312

BLAST of MS003884 vs. TAIR 10
Match: AT4G26260.2 (myo-inositol oxygenase 4 )

HSP 1 Score: 468.8 bits (1205), Expect = 3.8e-132
Identity = 230/327 (70.34%), Postives = 255/327 (77.98%), Query Frame = 0

Query: 10  EKKGAISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKSVEE 69
           EK G    + EL LDGGF +P   T     E F+APE+N+FG+ FRDY  ESERQK VEE
Sbjct: 18  EKSG--DNIGELKLDGGFSMPKMDT--NDDEAFLAPEMNAFGRQFRDYDVESERQKGVEE 77

Query: 70  FYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQT 129
           FYR+ HI+QT DFVKKMR +YGK+D++ MSIWECCELLN+VVD+SDPDLDEPQIQHLLQ+
Sbjct: 78  FYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQS 137

Query: 130 AEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLPSFG 189
           AEAIRKDYP++DWLHLTALIH                           DLGKV+ LP FG
Sbjct: 138 AEAIRKDYPNEDWLHLTALIH---------------------------DLGKVITLPQFG 197

Query: 190 GLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWG 249
           GLPQWAVVGDT P+GCAFDES VHHKYF EN D  N  YNTKNGIYSEGCGL+NVM+SWG
Sbjct: 198 GLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWG 257

Query: 250 HDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYD 309
           HDDYMYLVAKENGSTLPSAG FIIRYHSFYPLH +G Y HLMNEED +NLKWLH+FNKYD
Sbjct: 258 HDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYD 313

Query: 310 LYSKSKERIDVEKVKPYYMSLINKVNP 337
           LYSKSK  +DVEKVKPYYMSLI K  P
Sbjct: 318 LYSKSKVHVDVEKVKPYYMSLIKKYFP 313

BLAST of MS003884 vs. TAIR 10
Match: AT1G14520.1 (myo-inositol oxygenase 1 )

HSP 1 Score: 448.0 bits (1151), Expect = 6.9e-126
Identity = 222/330 (67.27%), Postives = 253/330 (76.67%), Query Frame = 0

Query: 8   VEEKKG-AISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 67
           VE+ +G    E TELVLD                GF AP  NSFG++FRDY AESER++ 
Sbjct: 19  VEKNQGNGKEEETELVLDA---------------GFEAPHTNSFGRTFRDYDAESERRRG 78

Query: 68  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 127
           VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+ +D+SDPDLDEPQI+HL
Sbjct: 79  VEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFIDESDPDLDEPQIEHL 138

Query: 128 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 187
           LQTAEAIRKDYPD+DWLHLT LIH                           DLGKVLL  
Sbjct: 139 LQTAEAIRKDYPDEDWLHLTGLIH---------------------------DLGKVLLHS 198

Query: 188 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 247
           SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++
Sbjct: 199 SFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLM 258

Query: 248 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 307
           SWGHDDYMYLVAKEN +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FN
Sbjct: 259 SWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFN 306

Query: 308 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSK R++VE+VKPYY+SL NK  P
Sbjct: 319 KYDLYSKSKVRVNVEEVKPYYLSLTNKYFP 306

BLAST of MS003884 vs. TAIR 10
Match: AT1G14520.2 (myo-inositol oxygenase 1 )

HSP 1 Score: 448.0 bits (1151), Expect = 6.9e-126
Identity = 222/330 (67.27%), Postives = 253/330 (76.67%), Query Frame = 0

Query: 8   VEEKKG-AISEMTELVLDGGFVIPDNQTAAAAAEGFVAPEINSFGQSFRDYAAESERQKS 67
           VE+ +G    E TELVLD                GF AP  NSFG++FRDY AESER++ 
Sbjct: 17  VEKNQGNGKEEETELVLDA---------------GFEAPHTNSFGRTFRDYDAESERRRG 76

Query: 68  VEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHL 127
           VEEFYR+NHI QT DFV+KMRE+Y K++R EMSIWECCELLN+ +D+SDPDLDEPQI+HL
Sbjct: 77  VEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFIDESDPDLDEPQIEHL 136

Query: 128 LQTAEAIRKDYPDQDWLHLTALIHGTIHLHKTECPLFSSVFSFLNDFALFSDLGKVLLLP 187
           LQTAEAIRKDYPD+DWLHLT LIH                           DLGKVLL  
Sbjct: 137 LQTAEAIRKDYPDEDWLHLTGLIH---------------------------DLGKVLLHS 196

Query: 188 SFGGLPQWAVVGDTHPLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMI 247
           SFG LPQWAVVGDT P+GCAFDESIVHHKYFKEN D +NP+YN+K GIY+EGCGLDNV++
Sbjct: 197 SFGELPQWAVVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLM 256

Query: 248 SWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFN 307
           SWGHDDYMYLVAKEN +TLPSAGLFIIRYHSFY LHKS AYKHLMN ED +N+KWL +FN
Sbjct: 257 SWGHDDYMYLVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFN 304

Query: 308 KYDLYSKSKERIDVEKVKPYYMSLINKVNP 337
           KYDLYSKSK R++VE+VKPYY+SL NK  P
Sbjct: 317 KYDLYSKSKVRVNVEEVKPYYLSLTNKYFP 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144825.11.2e-16789.49inositol oxygenase 4-like [Momordica charantia][more]
XP_023515618.11.5e-15482.42inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol ox... [more]
XP_022960634.18.1e-15382.73inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase ... [more]
XP_022987889.14.5e-15181.52inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase ... [more]
XP_008450805.12.7e-14879.57PREDICTED: inositol oxygenase 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
O822001.7e-13268.15Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q8H1S05.3e-13170.34Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
Q8L7999.7e-12567.27Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q5Z8T31.8e-12366.98Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
Q9FJU49.1e-12371.14Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CSQ85.7e-16889.49Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1[more]
A0A6J1HBL63.9e-15382.73Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1[more]
A0A6J1JI242.2e-15181.52Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1[more]
A0A1S3BQR61.3e-14879.57Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1[more]
A0A0A0LZA82.2e-14879.57Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G19800.11.2e-13368.15myo-inositol oxygenase 2 [more]
AT4G26260.13.8e-13270.34myo-inositol oxygenase 4 [more]
AT4G26260.23.8e-13270.34myo-inositol oxygenase 4 [more]
AT1G14520.16.9e-12667.27myo-inositol oxygenase 1 [more]
AT1G14520.26.9e-12667.27myo-inositol oxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 65..151
e-value: 3.2E-37
score: 128.4
coord: 173..336
e-value: 2.2E-77
score: 260.0
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 170..334
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 11..151
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 11..151
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 170..334
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 173..336
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 58..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003884.1MS003884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding