MS003821 (gene) Bitter gourd (TR) v1

Overview
NameMS003821
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationscaffold127: 761848 .. 765370 (+)
RNA-Seq ExpressionMS003821
SyntenyMS003821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCACACTCACGAAGCTTCAAGTGTACCCACAAAATTTCGACTGCCCAAGTACGAGGGATGGCCACCTGATCGGAGCTCTTGGACGGGGATCCCACCGGTTCGGTGTCGGAGCGTCGACGAGCCGTAGTGGGTTGTTCTTGAGAAAGTGTAGATCGTTCAGAGGCGAGGATGGTGGGGAGTTTGAGAAGGAAGATTGTAAAGGGAGAAAACACAGAAATTCGAGGTCGAAAGAGGTGAAAGTTAAGAAGGAAAGTCAGTTTTGGAAATCTTTGAAGGCTGATGTTTTGGGTAGGTTTAATTTGTGCTTCGCGTCGGATCTTGAGAATGGGAAGCTTATGGCAAAAATGGAGGCCTTGTTTTCTTCGGTGAGTTTTGCTGCGTGCTGCTCACTTTGAATTTCATATAAACTCTGTTTTTGAGTGATATCTTGTTGAGCGAATGTGCCCGCAATTTTATTCGACTGCTTTGGCGACTGTAACTTGAAGTATCATCATGAACTGGAAGTTTTTTTATTAGTTGAGTTATTTGTTGTAAGAATTTGAAATTTTAATTTTGGATAATTGGATATCAATAAAGGAGGAGGAACCTTCTGTAGGCTGCTAAGGCACAAAATTGAACCAAATTATACTTAAACTGTAAAGAAATAGAACTATTTTGATCTGTGGTTTTTGATGTAAAGTCATGGATCAAGGAGGACATGTGCTTTGGTCTTCATTGGAGACTGTAGTAGCATCACTAACTGTAGTTCCTTTATTATCTATACTGAATAGGCTGCAGTCCAAATCGGGAGGTATATCGTTACGATGATGAGCACTGGTGTCATACTTGCTGTTGGGTTTCAGTTATCAGGTATCCCATATTTTGAATGTTTCATTTGTCGTTAAGATCGTTGATGTTAATATGAATTCTGTTTCTTGAAAATTATTAAGGTGGAGATAGTCAGATGAACACATTGATTTGGTATAGCTGGCTTGGAGGAGTTATTATTGGGACTATGATTGGTGCTAACATGGTATTAGAGGAACACTGCAGAGCTGGTCCACGAAATGTCGTTATAACTGGAAGGTAATTAATCACTCACAAGCAATGTAGTTCATTGGCACAGAAATGAATGAAAACCAGCTGATCTTTGTTTTAATGCAATTGGTTACTTCAATTTTAGTGTTGGCTTATATGGTATTCACATACTATATGATTTAAATCAACTGCTATGTTGTTAACATGGACGGTTGACTATGCAGCACAAGAGGATTGGGAAAAGCACTAGCCAGAGAGTTCCTCCTCTCGGGAGACCGCGTGGTTGTGGCTTCTCGCAGGTGGTCCTCAGAATTAAAATGAAACTTCTGATACACAGTATCTCAATGTTTTTACCACTTGCTTACATTTGCATTTTCTGCTCAATGATTTTGTGAAGACAGCCCCGGGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATGGCCGGAAGTGGTTCTTGCAATACAAGCCTGGCACGTGCTAAAGTTGTTGGCACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTAAATGAACTTGGTTCTATTGACATTTGGGTGAGTCACGAACTTATGAAGTTAATTTTTTATGCACGAACTGGAATGGTTTTTGGCTCTGTCAGATAACATGCTGCTTTTATTGATTTTTTCTTTATAATAATATTTAGATTTTTAAATGTACTGGTTCATTCTTAAAGTAGCTGCCTTGGTGCCCTTTAGGAATAAAAGTCTGATCGTCTGAAAGCAAGAGATTCCTATTTAAAAACATTAAAATTTGAAATCGTCAAGGATAAAAGAGGCATACTCGTAGGCCATAGCTTATCTCTACACTGTCAATCTTGATTAGAAAAGAAAAAGAAATGTAAGAAATATGGATTTAGCCTTAATTCTCAATCTTGAATCTGTTTAATGTGACAAATTATGGACAGATAAACAACGCAGGGACCAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGGTAAAGTCATTAGTAATTCTGGATTTCTTTACCTATACTTCTCTTGATAAATCTGAACGTAATCTTCTCCAATAGATATGAGCTCTTTAAGAAGGTACTTAGTGGTTGCCAGTTTATTCCACTTTGCAATATCCCAGGCTCTGACTGTTACTAATTTTTTAAAATAAATGTTACTGGAGTTTTATTTGAATTCAAGTATTGATGGCTTAAAACTAGAGCATTGAAACTAGTCTCTTTCACTCTGTTTATGAATGGAAGGCATGGTTTACATTTCACATTCATCTTACGTCCTGGTTGAAAATTCATTTTGTCCCTTCTGATAAATGGTGACTTTGGAAGATTTGATGCCAGACCCATAAGTTGATTTAATTTCATCCCTGATTATTTGATAGATTCTCTCAACAAACCTCGTTGGTTCACTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAAATCAGGCTAAAGGAGGGCACATATTCAACATGGACGGTGCTGGCTCTGGAGGTTCGAGTACTCCTTTGACGGCTGTGTAAGTTCATTGCACACTGCAGAATGTAAATGTAGTTTTGCACAGGGCGGGAGGTTTCATTTCGAATTCTGGTTACTGGTTTTAACTCGTGGAACCATTCATATCCTCATATCTATGAATTCAACAGTCGGTTTTGGTTTTTTACGACCTTTTATTTACTGAACTTGTAGGTATGGATCGACAAAATGTGGTCTTAGACAGCTTCAGTCATCTCTTCTGAAAGAGTGCAGGCGATCCAATGTAGGAGTACACACCGCATCTCCCGGCATGGTTTTGACAGATCTCCTTCTTAGGTGAATCAAGCTTCTTCTTGTCCTCGTTTCTCCATTTGCAGATAAATTGCTAGAAAAACACATTTTTCTGGATAAACATTCTCCATTGGAATAATTCTAACGAAAACTTGCCCTGTACTACTTGTGGCTTGCATACAGTGGTTCAACGGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTTGCTAGAACATTAGTTCCTCGGATGCGAGTTGTTAAAGGAACGGGAAAAGCCATCAACTACTTAACTCCTCCAAGAATCTTGTTGGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAGCAGGTAAGTTCAGTCCCATCATGGAAACTAGCAAACACAAATACTTGTAGTATTCAAAAATCCATTGAGAACCCAGATGAGCAATCATGGAGAGTTGTGCTTGTTATTCATTCAGGCTGTAATATATTGCAGGGGAGGGCTCTGTATGCAGCGGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACCGATGCAATGGAGATGTACACAGAGAACACTTGGGTGTCTGTCTTCTCACTCTCAGTCGTATGCGCCTTCATAATCCTCTCTACAACAAACAGCACTCTTCCCGGAACT

mRNA sequence

ATGACCACACTCACGAAGCTTCAAGTGTACCCACAAAATTTCGACTGCCCAAGTACGAGGGATGGCCACCTGATCGGAGCTCTTGGACGGGGATCCCACCGGTTCGGTGTCGGAGCGTCGACGAGCCGTAGTGGGTTGTTCTTGAGAAAGTGTAGATCGTTCAGAGGCGAGGATGGTGGGGAGTTTGAGAAGGAAGATTGTAAAGGGAGAAAACACAGAAATTCGAGGTCGAAAGAGGTGAAAGTTAAGAAGGAAAGTCAGTTTTGGAAATCTTTGAAGGCTGATGTTTTGGGTAGGTTTAATTTGTGCTTCGCGTCGGATCTTGAGAATGGGAAGCTTATGGCAAAAATGGAGGCCTTGTTTTCTTCGGCTGCAGTCCAAATCGGGAGGTATATCGTTACGATGATGAGCACTGGTGTCATACTTGCTGTTGGGTTTCAGTTATCAGGTGGAGATAGTCAGATGAACACATTGATTTGGTATAGCTGGCTTGGAGGAGTTATTATTGGGACTATGATTGGTGCTAACATGGTATTAGAGGAACACTGCAGAGCTGGTCCACGAAATGTCGTTATAACTGGAAGCACAAGAGGATTGGGAAAAGCACTAGCCAGAGAGTTCCTCCTCTCGGGAGACCGCGTGGTTGTGGCTTCTCGCAGCCCCGGGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATGGCCGGAAGTGGTTCTTGCAATACAAGCCTGGCACGTGCTAAAGTTGTTGGCACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTAAATGAACTTGGTTCTATTGACATTTGGATAAACAACGCAGGGACCAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGATTCTCTCAACAAACCTCGTTGGTTCACTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAAATCAGGCTAAAGGAGGGCACATATTCAACATGGACGGTGCTGGCTCTGGAGGTTCGAGTACTCCTTTGACGGCTGTGTATGGATCGACAAAATGTGGTCTTAGACAGCTTCAGTCATCTCTTCTGAAAGAGTGCAGGCGATCCAATGTAGGAGTACACACCGCATCTCCCGGCATGGTTTTGACAGATCTCCTTCTTAGTGGTTCAACGGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTTGCTAGAACATTAGTTCCTCGGATGCGAGTTGTTAAAGGAACGGGAAAAGCCATCAACTACTTAACTCCTCCAAGAATCTTGTTGGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAGCAGGGGAGGGCTCTGTATGCAGCGGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACCGATGCAATGGAGATGTACACAGAGAACACTTGGGTGTCTGTCTTCTCACTCTCAGTCGTATGCGCCTTCATAATCCTCTCTACAACAAACAGCACTCTTCCCGGAACT

Coding sequence (CDS)

ATGACCACACTCACGAAGCTTCAAGTGTACCCACAAAATTTCGACTGCCCAAGTACGAGGGATGGCCACCTGATCGGAGCTCTTGGACGGGGATCCCACCGGTTCGGTGTCGGAGCGTCGACGAGCCGTAGTGGGTTGTTCTTGAGAAAGTGTAGATCGTTCAGAGGCGAGGATGGTGGGGAGTTTGAGAAGGAAGATTGTAAAGGGAGAAAACACAGAAATTCGAGGTCGAAAGAGGTGAAAGTTAAGAAGGAAAGTCAGTTTTGGAAATCTTTGAAGGCTGATGTTTTGGGTAGGTTTAATTTGTGCTTCGCGTCGGATCTTGAGAATGGGAAGCTTATGGCAAAAATGGAGGCCTTGTTTTCTTCGGCTGCAGTCCAAATCGGGAGGTATATCGTTACGATGATGAGCACTGGTGTCATACTTGCTGTTGGGTTTCAGTTATCAGGTGGAGATAGTCAGATGAACACATTGATTTGGTATAGCTGGCTTGGAGGAGTTATTATTGGGACTATGATTGGTGCTAACATGGTATTAGAGGAACACTGCAGAGCTGGTCCACGAAATGTCGTTATAACTGGAAGCACAAGAGGATTGGGAAAAGCACTAGCCAGAGAGTTCCTCCTCTCGGGAGACCGCGTGGTTGTGGCTTCTCGCAGCCCCGGGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATGGCCGGAAGTGGTTCTTGCAATACAAGCCTGGCACGTGCTAAAGTTGTTGGCACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTAAATGAACTTGGTTCTATTGACATTTGGATAAACAACGCAGGGACCAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGATTCTCTCAACAAACCTCGTTGGTTCACTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAAATCAGGCTAAAGGAGGGCACATATTCAACATGGACGGTGCTGGCTCTGGAGGTTCGAGTACTCCTTTGACGGCTGTGTATGGATCGACAAAATGTGGTCTTAGACAGCTTCAGTCATCTCTTCTGAAAGAGTGCAGGCGATCCAATGTAGGAGTACACACCGCATCTCCCGGCATGGTTTTGACAGATCTCCTTCTTAGTGGTTCAACGGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTTGCTAGAACATTAGTTCCTCGGATGCGAGTTGTTAAAGGAACGGGAAAAGCCATCAACTACTTAACTCCTCCAAGAATCTTGTTGGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAGCAGGGGAGGGCTCTGTATGCAGCGGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACCGATGCAATGGAGATGTACACAGAGAACACTTGGGTGTCTGTCTTCTCACTCTCAGTCGTATGCGCCTTCATAATCCTCTCTACAACAAACAGCACTCTTCCCGGAACT

Protein sequence

MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGGEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEALFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMAGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT
Homology
BLAST of MS003821 vs. NCBI nr
Match: XP_022145058.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Momordica charantia])

HSP 1 Score: 1021.9 bits (2641), Expect = 1.9e-294
Identity = 517/520 (99.42%), Postives = 517/520 (99.42%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKLQ YPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSR GLFLRKCRSFRGEDGG
Sbjct: 1   MTTLTKLQAYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRGGLFLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL
Sbjct: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSP SVQETLRELEENLKGLMA
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPRSVQETLRELEENLKGLMA 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 520

BLAST of MS003821 vs. NCBI nr
Match: XP_038880096.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Benincasa hispida])

HSP 1 Score: 940.3 bits (2429), Expect = 7.3e-270
Identity = 480/522 (91.95%), Postives = 493/522 (94.44%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFD--CPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGED 60
           MTTLTKL VYPQ+F+   PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRG D
Sbjct: 1   MTTLTKLHVYPQSFEGPTPSTRDGHLIGALGRGIHRFGVGVSTSRSGLCLRKCRSFRGGD 60

Query: 61  GGEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKME 120
           GG+FEKED KGR  RNSR KEVK+KKESQFWK L++ VLG+FNL   SD++ GKLMA ME
Sbjct: 61  GGDFEKEDSKGRNRRNSRLKEVKMKKESQFWKFLRSGVLGKFNLLLGSDVDQGKLMANME 120

Query: 121 ALFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMV 180
            L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGD+QMN LIWYSWLGGVIIGTMIGANMV
Sbjct: 121 GLLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDNQMNALIWYSWLGGVIIGTMIGANMV 180

Query: 181 LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGL 240
           LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSP SVQETLRELEENLKGL
Sbjct: 181 LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGL 240

Query: 241 MAGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLL 300
           M  SGS N SLA AKVVGTTCNVCDPEDVHNLANFA+NELGSIDIWINNAGTNKGFRPLL
Sbjct: 241 MVRSGSSNKSLACAKVVGTTCNVCDPEDVHNLANFAINELGSIDIWINNAGTNKGFRPLL 300

Query: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360
           QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST
Sbjct: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360

Query: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420
           KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART
Sbjct: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420

Query: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480
           LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR
Sbjct: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480

Query: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN+T PGT
Sbjct: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTFPGT 522

BLAST of MS003821 vs. NCBI nr
Match: XP_023529434.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 930.6 bits (2404), Expect = 5.8e-267
Identity = 473/520 (90.96%), Postives = 489/520 (94.04%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKL VYPQ+FD PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLRLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           ++EKED KGR   +SR K+V  KKE QFWK L++ VLG+ NL   SD E GKLMA MEAL
Sbjct: 61  DYEKEDRKGRSRGDSRLKKVSTKKEIQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDS MNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSHMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSP SVQET+RELEENLKGLM 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
            SGS NTSL  AKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++G+ALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT++TLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of MS003821 vs. NCBI nr
Match: KAG6587961.1 (putative chlorophyll(ide) b reductase NYC1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 929.1 bits (2400), Expect = 1.7e-266
Identity = 472/520 (90.77%), Postives = 490/520 (94.23%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKL V PQ+FD PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           ++EKED KGR   +SR K+V  KKE+QFWK L++ VLG+ NL   SD E GKLMA MEAL
Sbjct: 61  DYEKEDRKGRSRGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIG+MIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGSMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSP SVQET+RELEENLKGLM 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
            SGS NTSL  AKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++G+ALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT++TLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of MS003821 vs. NCBI nr
Match: XP_022933701.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 927.9 bits (2397), Expect = 3.7e-266
Identity = 472/520 (90.77%), Postives = 490/520 (94.23%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKL V PQ+FD PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           ++EKED KGR   +SR K+V  KKE+QFWK L++ VLG+ NL   SD E GKLMA MEAL
Sbjct: 61  DYEKEDRKGRSGGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSP SVQET+RELEENLKGLM 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
            SGS NTSL  AKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++G+ALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT++TLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of MS003821 vs. ExPASy Swiss-Prot
Match: Q5N800 (Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NYC1 PE=1 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.8e-191
Identity = 352/480 (73.33%), Postives = 398/480 (82.92%), Query Frame = 0

Query: 48  LRKCRSFRGE--DGGEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLG----RFN 107
           L +CR+F+ E  +GGE               + E + +++   +K LKA + G    R  
Sbjct: 31  LLRCRAFKQEADNGGEEASS-----SPPPPTTAEARRRRKGPLYK-LKAAIQGLAGSRSA 90

Query: 108 LCFASDLENGKLMAKMEALFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWY 167
              A   E  + + K E +F S A Q+GRY++TMMS+GV+L VGFQLSGGDSQMNTLIWY
Sbjct: 91  AAEAYGGEYQRAVEKAEEIFFSVATQVGRYVITMMSSGVVLGVGFQLSGGDSQMNTLIWY 150

Query: 168 SWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSP 227
           SWLGGVIIGTMIGAN VLEEHC+AGPRNVVITGSTRGLGKALAREFLLSGDRVV+ASRSP
Sbjct: 151 SWLGGVIIGTMIGANSVLEEHCKAGPRNVVITGSTRGLGKALAREFLLSGDRVVIASRSP 210

Query: 228 GSVQETLRELEENL-KGLMAGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGS 287
            SV +T+ ELEEN+ +GL          L  AKVVGT+C+VC PEDV  L NFA +ELGS
Sbjct: 211 ESVLQTINELEENIQEGLSVAKKKQREILLHAKVVGTSCDVCKPEDVKKLVNFAKDELGS 270

Query: 288 IDIWINNAGTNKGFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMD 347
           IDIWINNAGTNKGFRPL+ F+DEDI+QI+STNLVGSLLCTREAM VM++Q KGGH+FNMD
Sbjct: 271 IDIWINNAGTNKGFRPLVNFSDEDISQIVSTNLVGSLLCTREAMNVMQHQQKGGHVFNMD 330

Query: 348 GAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVR 407
           GAGSGGSSTPLTAVYGSTKCGLRQ Q+SLLKE RRS VGVHTASPGMVLTDLLLSGS++R
Sbjct: 331 GAGSGGSSTPLTAVYGSTKCGLRQFQASLLKESRRSKVGVHTASPGMVLTDLLLSGSSLR 390

Query: 408 NKQMFNIICELPETVARTLVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQG 467
           NKQMFN+ICELPETVARTLVPRMRVVKG+GKAINYLTPPRILLALVTAW+RRGRWFDE+G
Sbjct: 391 NKQMFNLICELPETVARTLVPRMRVVKGSGKAINYLTPPRILLALVTAWVRRGRWFDEEG 450

Query: 468 RALYAAEADRIRNWAENRTRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           RA+YAAEADRIRNWAE+R RFSFTDAMEMYTENTWVSVFSLSVVCAFIILS++   LPGT
Sbjct: 451 RAVYAAEADRIRNWAESRARFSFTDAMEMYTENTWVSVFSLSVVCAFIILSSSGGPLPGT 504

BLAST of MS003821 vs. ExPASy Swiss-Prot
Match: Q93ZA0 (Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NYC1 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.3e-189
Identity = 352/523 (67.30%), Postives = 411/523 (78.59%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCP-STRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDG 60
           MTTLTK+QVYPQ  +     RD   +G+      R      ++R  +++ +C        
Sbjct: 1   MTTLTKIQVYPQVLEHRLFFRDPIRVGS------RLTCRERSNR--VYVHRC-------- 60

Query: 61  GEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEA 120
                      + +  R ++V+  K +  W SLK+  LG   L F S  E  + +  +E 
Sbjct: 61  -----------EKKVERKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEM 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           +FSS AVQI RYIVTM STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVL
Sbjct: 121 VFSSVAVQIARYIVTMTSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLM 240
           E+H RAGPRNVVITGSTRGLGKALAREFLLSGDRV+V SRS  SV  T++ELE+NLK +M
Sbjct: 181 EDHYRAGPRNVVITGSTRGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIM 240

Query: 241 A-GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLL 300
           +  S S    L+ AKVVG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL
Sbjct: 241 SNASESARKKLSDAKVVGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLL 300

Query: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360
           +FT+EDITQI+STNL+GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGST
Sbjct: 301 EFTEEDITQIVSTNLIGSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGST 360

Query: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420
           KCGLRQ   S++KE +++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVART
Sbjct: 361 KCGLRQFHGSIVKESQKTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVART 420

Query: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480
           LVPRMRVVKG+GKA+NYLTPPRILLA+VT+WLRRGRWFD+QGRALYAAEADR+RNWAENR
Sbjct: 421 LVPRMRVVKGSGKAVNYLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENR 480

Query: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIIL-STTNSTLPGT 521
           TR S TDAMEMYTENTWVSVFSLSVVCAFIIL STT S+ PGT
Sbjct: 481 TRLSLTDAMEMYTENTWVSVFSLSVVCAFIILQSTTPSSFPGT 496

BLAST of MS003821 vs. ExPASy Swiss-Prot
Match: Q8LEU3 (Chlorophyll(ide) b reductase NOL, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NOL PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.9e-51
Identity = 119/285 (41.75%), Postives = 167/285 (58.60%), Query Frame = 0

Query: 187 PRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMAGSGSCN 246
           P N++ITGST+G+G ALAREFL +GD VV+ SRS   V+  ++ L+E             
Sbjct: 79  PYNILITGSTKGIGYALAREFLKAGDNVVICSRSAERVETAVQSLKEEF----------- 138

Query: 247 TSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNK-GFRPLLQFTDEDI 306
                  V GT C+V + +DV  L  ++   L  IDIWINNAG+N   F+PL + +DED+
Sbjct: 139 ----GEHVWGTKCDVTEGKDVRELVAYSQKNLKYIDIWINNAGSNAYSFKPLAEASDEDL 198

Query: 307 TQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQL 366
            +++ TN +G +LC REAM +M  Q++GGHIFN+DGAGS G  TP  A YG+TK  +  L
Sbjct: 199 IEVVKTNTLGLMLCCREAMNMMLTQSRGGHIFNIDGAGSDGRPTPRFAAYGATKRSVVHL 258

Query: 367 QSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRNKQMF-NIICELPETVARTLVP 426
             SL  E +     NV VH  SPGMV TDLL+SG+T +  + F N++ E  E VA  LVP
Sbjct: 259 TKSLQAELQMQDVKNVVVHNLSPGMVTTDLLMSGATTKQAKFFINVLAEPAEVVAEYLVP 318

Query: 427 RMRVVKGTGKA----INYLTPPRI---LLALVTAWLRRGRWFDEQ 460
            +R +  +G      I +LT  +    + + V    R+ R+  E+
Sbjct: 319 NIRAIPASGSMKPTYIRFLTGIKAYTKIFSRVALGARKNRYVTEE 348

BLAST of MS003821 vs. ExPASy Swiss-Prot
Match: Q84ST4 (Chlorophyll(ide) b reductase NOL, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NOL PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.6e-49
Identity = 111/266 (41.73%), Postives = 160/266 (60.15%), Query Frame = 0

Query: 166 GVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQ 225
           G+    + G   V        P NV+ITGST+G+G ALA+EFL +GD VV+ SRS   V+
Sbjct: 52  GIRAEAVPGGGGVARRAAMVPPYNVLITGSTKGIGYALAKEFLKAGDNVVICSRSAERVE 111

Query: 226 ETLRELEENLKGLMAGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWI 285
             + +L++                    V G  C+V + +DV  L +FA +++  IDIWI
Sbjct: 112 SAVTDLKK--------------EFGEQHVWGIVCDVREGKDVKALVDFARDKMKYIDIWI 171

Query: 286 NNAGTNK-GFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGS 345
           NNAG+N   ++PL++ +DE + ++++TN +G ++C REA+ +MRNQ +GGHIFN+DGAGS
Sbjct: 172 NNAGSNAYSYKPLVETSDEALMEVITTNTLGLMICCREAINMMRNQPRGGHIFNIDGAGS 231

Query: 346 GGSSTPLTAVYGSTKCGLRQLQSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRN 405
            G  TP  A YG+TK  +  L  SL  E +    +NV VH  SPGMV TDLL+SG+T + 
Sbjct: 232 DGRPTPRFAAYGATKRSVVHLTKSLQAELQMNEVNNVMVHNLSPGMVTTDLLMSGATTKQ 291

Query: 406 KQMF-NIICELPETVARTLVPRMRVV 427
            + F NI+ E    VA  LVP +R +
Sbjct: 292 AKFFINILAEPANVVADYLVPNIRAI 303

BLAST of MS003821 vs. ExPASy Swiss-Prot
Match: P16542 (Granaticin polyketide synthase putative ketoacyl reductase 1 OS=Streptomyces violaceoruber OX=1935 GN=gra-orf5 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.8e-14
Identity = 62/204 (30.39%), Postives = 97/204 (47.55%), Query Frame = 0

Query: 191 VITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMAGSGSCNTSLA 250
           ++TG+T G+G A+AR     G R  + +R    + +T++EL         G G       
Sbjct: 21  LVTGATSGIGLAIARRLAALGARTFLCARDEERLAQTVKELR--------GEG------- 80

Query: 251 RAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILS 310
              V GT C+V DP  +      A+   G++DI +NNAG + G     +  DE    +++
Sbjct: 81  -FDVDGTVCDVADPAQIRAYVAAAVQRYGTVDILVNNAGRSGG-GATAEIADELWLDVIT 140

Query: 311 TNLVGSLLCTREAMRVMRNQA-KGGHIFNMDGAGSGGSSTPLTAV-YGSTKCGLRQLQSS 370
           TNL    L T+E +      A K G I N+  A +GG    + AV Y ++K G+  L  +
Sbjct: 141 TNLTSVFLMTKEVLNAGGMLAKKRGRIINI--ASTGGKQGVVHAVPYSASKHGVVGLTKA 200

Query: 371 LLKECRRSNVGVHTASPGMVLTDL 393
           L  E  R+ + V+   PG V T +
Sbjct: 201 LGLELARTGITVNAVCPGFVETPM 205

BLAST of MS003821 vs. ExPASy TrEMBL
Match: A0A6J1CUX5 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014576 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 9.2e-295
Identity = 517/520 (99.42%), Postives = 517/520 (99.42%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKLQ YPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSR GLFLRKCRSFRGEDGG
Sbjct: 1   MTTLTKLQAYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRGGLFLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL
Sbjct: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSP SVQETLRELEENLKGLMA
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPRSVQETLRELEENLKGLMA 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 520

BLAST of MS003821 vs. ExPASy TrEMBL
Match: A0A6J1EZT2 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111441038 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.8e-266
Identity = 472/520 (90.77%), Postives = 490/520 (94.23%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKL V PQ+FD PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           ++EKED KGR   +SR K+V  KKE+QFWK L++ VLG+ NL   SD E GKLMA MEAL
Sbjct: 61  DYEKEDRKGRSGGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSP SVQET+RELEENLKGLM 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
            SGS NTSL  AKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++G+ALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT++TLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of MS003821 vs. ExPASy TrEMBL
Match: A0A6J1HLL1 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465318 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 472/520 (90.77%), Postives = 487/520 (93.65%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDGG 60
           MTTLTKL V PQ+FD PSTRDGHLIGALGRG HRFGVG STSRSGL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  EFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEAL 120
           + EKED KGR   +SR K+V  KKE+QFWK L++  LG+ NL   SD E GKLMA MEAL
Sbjct: 61  DNEKEDRKGRSRGDSRLKKVSTKKEAQFWKFLRSGFLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSP SVQET+RELEENLKGLM 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMI 240

Query: 241 GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
            SGS NTSL  AKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVH ASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHAASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDE+G+ALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN+TLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 520

BLAST of MS003821 vs. ExPASy TrEMBL
Match: A0A1S3CJ66 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501561 PE=4 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.3e-264
Identity = 473/521 (90.79%), Postives = 485/521 (93.09%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRS-GLFLRKCRSFRGEDG 60
           MTTL KL VYPQ F+ PSTRDGHLIGALG G HR  VG STSR  GL LRKCRSFRG DG
Sbjct: 1   MTTLAKLHVYPQTFEGPSTRDGHLIGALGPGVHRLDVGVSTSRGRGLCLRKCRSFRGGDG 60

Query: 61  GEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEA 120
           G+FEKE+ KG   RNSR KEVK+KKESQFWK L++ VLG+FNL   SD++ GKLMA ME 
Sbjct: 61  GDFEKENSKGGNRRNSRLKEVKMKKESQFWKFLRSGVLGKFNLLMGSDVDQGKLMANMEG 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL
Sbjct: 121 LLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLM 240
           EEHC AGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRS  SVQETLRELEENLKGLM
Sbjct: 181 EEHCSAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSSESVQETLRELEENLKGLM 240

Query: 241 AGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300
             SGS N SLA AKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ
Sbjct: 241 VRSGSSNKSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300

Query: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360
           FTDEDITQILSTNLVGSLLCTREAMRVMRNQ KGGHIFNMDGAGSGGSSTPLTAVYGSTK
Sbjct: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQPKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360

Query: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420
           CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL
Sbjct: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420

Query: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRT 480
           VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD+QGRALYAAEADRIRNWAENRT
Sbjct: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDQGRALYAAEADRIRNWAENRT 480

Query: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN+TLPGT
Sbjct: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTLPGT 521

BLAST of MS003821 vs. ExPASy TrEMBL
Match: A0A5D3DZ70 (Putative chlorophyll(Ide) b reductase NYC1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00120 PE=4 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.8e-264
Identity = 472/521 (90.60%), Postives = 484/521 (92.90%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCPSTRDGHLIGALGRGSHRFGVGASTSRS-GLFLRKCRSFRGEDG 60
           MTTL KL VYPQ F+ PSTRDGHLIGA G G HR  VG STSR  GL LRKCRSFRG DG
Sbjct: 1   MTTLAKLHVYPQTFEGPSTRDGHLIGARGPGVHRLDVGVSTSRGRGLCLRKCRSFRGGDG 60

Query: 61  GEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEA 120
           G+FEKE+ KG   RNSR KEVK+KKESQFWK L++ VLG+FNL   SD++ GKLMA ME 
Sbjct: 61  GDFEKENSKGGNRRNSRLKEVKIKKESQFWKFLRSGVLGKFNLLMGSDVDQGKLMANMEG 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL
Sbjct: 121 LLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLM 240
           EEHC AGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRS  SVQETLRELEENLKGLM
Sbjct: 181 EEHCSAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSSESVQETLRELEENLKGLM 240

Query: 241 AGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300
             SGS N SLA AKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ
Sbjct: 241 VRSGSSNKSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300

Query: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360
           FTDEDITQILSTNLVGSLLCTREAMRVMRNQ KGGHIFNMDGAGSGGSSTPLTAVYGSTK
Sbjct: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQPKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360

Query: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420
           CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL
Sbjct: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420

Query: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENRT 480
           VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD+QGRALYAAEADRIRNWAENRT
Sbjct: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDQGRALYAAEADRIRNWAENRT 480

Query: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNSTLPGT 521
           RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN+TLPGT
Sbjct: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTLPGT 521

BLAST of MS003821 vs. TAIR 10
Match: AT4G13250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 663.3 bits (1710), Expect = 1.6e-190
Identity = 352/523 (67.30%), Postives = 411/523 (78.59%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCP-STRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDG 60
           MTTLTK+QVYPQ  +     RD   +G+      R      ++R  +++ +C        
Sbjct: 1   MTTLTKIQVYPQVLEHRLFFRDPIRVGS------RLTCRERSNR--VYVHRC-------- 60

Query: 61  GEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEA 120
                      + +  R ++V+  K +  W SLK+  LG   L F S  E  + +  +E 
Sbjct: 61  -----------EKKVERKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEM 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           +FSS AVQI RYIVTM STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVL
Sbjct: 121 VFSSVAVQIARYIVTMTSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLM 240
           E+H RAGPRNVVITGSTRGLGKALAREFLLSGDRV+V SRS  SV  T++ELE+NLK +M
Sbjct: 181 EDHYRAGPRNVVITGSTRGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIM 240

Query: 241 A-GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLL 300
           +  S S    L+ AKVVG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL
Sbjct: 241 SNASESARKKLSDAKVVGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLL 300

Query: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360
           +FT+EDITQI+STNL+GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGST
Sbjct: 301 EFTEEDITQIVSTNLIGSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGST 360

Query: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420
           KCGLRQ   S++KE +++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVART
Sbjct: 361 KCGLRQFHGSIVKESQKTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVART 420

Query: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480
           LVPRMRVVKG+GKA+NYLTPPRILLA+VT+WLRRGRWFD+QGRALYAAEADR+RNWAENR
Sbjct: 421 LVPRMRVVKGSGKAVNYLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENR 480

Query: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIIL-STTNSTLPGT 521
           TR S TDAMEMYTENTWVSVFSLSVVCAFIIL STT S+ PGT
Sbjct: 481 TRLSLTDAMEMYTENTWVSVFSLSVVCAFIILQSTTPSSFPGT 496

BLAST of MS003821 vs. TAIR 10
Match: AT4G13250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 654.4 bits (1687), Expect = 7.4e-188
Identity = 350/523 (66.92%), Postives = 409/523 (78.20%), Query Frame = 0

Query: 1   MTTLTKLQVYPQNFDCP-STRDGHLIGALGRGSHRFGVGASTSRSGLFLRKCRSFRGEDG 60
           MTTLTK+QVYPQ  +     RD   +G+      R      ++R  +++ +C        
Sbjct: 1   MTTLTKIQVYPQVLEHRLFFRDPIRVGS------RLTCRERSNR--VYVHRC-------- 60

Query: 61  GEFEKEDCKGRKHRNSRSKEVKVKKESQFWKSLKADVLGRFNLCFASDLENGKLMAKMEA 120
                      + +  R ++V+  K +  W SLK+  LG   L F S  E  + +  +E 
Sbjct: 61  -----------EKKVERKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEM 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           +FSS AVQI RYIVTM STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVL
Sbjct: 121 VFSSVAVQIARYIVTMTSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLM 240
           E+H RAGPRNVVITG  RGLGKALAREFLLSGDRV+V SRS  SV  T++ELE+NLK +M
Sbjct: 181 EDHYRAGPRNVVITG--RGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIM 240

Query: 241 A-GSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLL 300
           +  S S    L+ AKVVG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL
Sbjct: 241 SNASESARKKLSDAKVVGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLL 300

Query: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360
           +FT+EDITQI+STNL+GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGST
Sbjct: 301 EFTEEDITQIVSTNLIGSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGST 360

Query: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420
           KCGLRQ   S++KE +++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVART
Sbjct: 361 KCGLRQFHGSIVKESQKTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVART 420

Query: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480
           LVPRMRVVKG+GKA+NYLTPPRILLA+VT+WLRRGRWFD+QGRALYAAEADR+RNWAENR
Sbjct: 421 LVPRMRVVKGSGKAVNYLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENR 480

Query: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIIL-STTNSTLPGT 521
           TR S TDAMEMYTENTWVSVFSLSVVCAFIIL STT S+ PGT
Sbjct: 481 TRLSLTDAMEMYTENTWVSVFSLSVVCAFIILQSTTPSSFPGT 494

BLAST of MS003821 vs. TAIR 10
Match: AT5G04900.1 (NYC1-like )

HSP 1 Score: 203.8 bits (517), Expect = 3.5e-52
Identity = 119/285 (41.75%), Postives = 167/285 (58.60%), Query Frame = 0

Query: 187 PRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEENLKGLMAGSGSCN 246
           P N++ITGST+G+G ALAREFL +GD VV+ SRS   V+  ++ L+E             
Sbjct: 79  PYNILITGSTKGIGYALAREFLKAGDNVVICSRSAERVETAVQSLKEEF----------- 138

Query: 247 TSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNK-GFRPLLQFTDEDI 306
                  V GT C+V + +DV  L  ++   L  IDIWINNAG+N   F+PL + +DED+
Sbjct: 139 ----GEHVWGTKCDVTEGKDVRELVAYSQKNLKYIDIWINNAGSNAYSFKPLAEASDEDL 198

Query: 307 TQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQL 366
            +++ TN +G +LC REAM +M  Q++GGHIFN+DGAGS G  TP  A YG+TK  +  L
Sbjct: 199 IEVVKTNTLGLMLCCREAMNMMLTQSRGGHIFNIDGAGSDGRPTPRFAAYGATKRSVVHL 258

Query: 367 QSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRNKQMF-NIICELPETVARTLVP 426
             SL  E +     NV VH  SPGMV TDLL+SG+T +  + F N++ E  E VA  LVP
Sbjct: 259 TKSLQAELQMQDVKNVVVHNLSPGMVTTDLLMSGATTKQAKFFINVLAEPAEVVAEYLVP 318

Query: 427 RMRVVKGTGKA----INYLTPPRI---LLALVTAWLRRGRWFDEQ 460
            +R +  +G      I +LT  +    + + V    R+ R+  E+
Sbjct: 319 NIRAIPASGSMKPTYIRFLTGIKAYTKIFSRVALGARKNRYVTEE 348

BLAST of MS003821 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 3.2e-13
Identity = 56/204 (27.45%), Postives = 98/204 (48.04%), Query Frame = 0

Query: 190 VVITGSTRGLGKALAREFLLSGDRVVV-ASRSPGSVQETLRELEENLKGLMAGSGSCNTS 249
           VVITG++RG+GKA+A     +G +V+V  +RS    +E  +++EE     +   G     
Sbjct: 79  VVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIEEYGGQAITFGG----- 138

Query: 250 LARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQI 309
                      +V    DV  +   AL++ G+ID+ +NNAG  +    L++       ++
Sbjct: 139 -----------DVSKATDVDAMMKTALDKWGTIDVVVNNAGITRD-TLLIRMKQSQWDEV 198

Query: 310 LSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSS 369
           ++ NL G  LCT+ A+++M  + K G I N+     G       A Y + K G+     +
Sbjct: 199 IALNLTGVFLCTQAAVKIMMKK-KRGRIINISSV-VGLIGNIGQANYAAAKGGVISFSKT 258

Query: 370 LLKECRRSNVGVHTASPGMVLTDL 393
             +E    N+ V+   PG + +D+
Sbjct: 259 AAREGASRNINVNVVCPGFIASDM 263

BLAST of MS003821 vs. TAIR 10
Match: AT3G55310.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.3e-11
Identity = 52/250 (20.80%), Postives = 105/250 (42.00%), Query Frame = 0

Query: 179 LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPGSVQETLRELEE-NLKG 238
           LE  C    + V++TG++ G+G+ +  +   +G +V+ A+R    +     E+   +  G
Sbjct: 11  LEPWCELKDKVVLVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINSFSSTG 70

Query: 239 LMAGSGSCNTSLARAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPL 298
           + A +   + S              D   +      A +  G ID  INNAG     +  
Sbjct: 71  IQAAALELDVS-------------SDAATIQKAVREAWDIFGKIDALINNAGIRGNVKLS 130

Query: 299 LQFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGS 358
           L  ++++   + +TNL G  L  +    +MR+  +GG + N+       S  P    Y  
Sbjct: 131 LDLSEDEWDNVFNTNLKGPWLVAKYVCVLMRDAKRGGSVINISSVAGVRSIVPGGLAYSC 190

Query: 359 TKCGLRQLQSSLLKECRRSNVGVHTASPGMV---LTDLLLSGSTVRNKQMFNIICELPET 418
           +K G+  +   +  E     + V++ +PG+    +T  L+    ++N     +  ++ +T
Sbjct: 191 SKGGVDTMSRMMAIELGVHKIRVNSIAPGLFKSEITQALMQKEWLKNVTERTVPLKVQQT 247

Query: 419 VARTLVPRMR 425
           +   L   +R
Sbjct: 251 IDPGLTSLVR 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145058.11.9e-29499.42probable chlorophyll(ide) b reductase NYC1, chloroplastic [Momordica charantia][more]
XP_038880096.17.3e-27091.95probable chlorophyll(ide) b reductase NYC1, chloroplastic [Benincasa hispida][more]
XP_023529434.15.8e-26790.96probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita pepo subsp.... [more]
KAG6587961.11.7e-26690.77putative chlorophyll(ide) b reductase NYC1, chloroplastic, partial [Cucurbita ar... [more]
XP_022933701.13.7e-26690.77probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q5N8001.8e-19173.33Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Oryza sativa subsp.... [more]
Q93ZA02.3e-18967.30Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Arabidopsis thalian... [more]
Q8LEU34.9e-5141.75Chlorophyll(ide) b reductase NOL, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q84ST44.6e-4941.73Chlorophyll(ide) b reductase NOL, chloroplastic OS=Oryza sativa subsp. japonica ... [more]
P165422.8e-1430.39Granaticin polyketide synthase putative ketoacyl reductase 1 OS=Streptomyces vio... [more]
Match NameE-valueIdentityDescription
A0A6J1CUX59.2e-29599.42probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Momordica charantia... [more]
A0A6J1EZT21.8e-26690.77probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita moschata ... [more]
A0A6J1HLL11.2e-26590.77probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita maxima OX... [more]
A0A1S3CJ661.3e-26490.79probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucumis melo OX=365... [more]
A0A5D3DZ703.8e-26490.60Putative chlorophyll(Ide) b reductase NYC1 OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT4G13250.11.6e-19067.30NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G13250.27.4e-18866.92NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G04900.13.5e-5241.75NYC1-like [more]
AT1G24360.13.2e-1327.45NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55310.11.3e-1120.80NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 355..374
score: 26.6
coord: 376..393
score: 28.91
coord: 189..206
score: 40.75
coord: 279..290
score: 52.58
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 189..392
e-value: 8.5E-34
score: 116.7
NoneNo IPR availableGENE3D3.40.50.720coord: 183..439
e-value: 2.2E-47
score: 163.3
NoneNo IPR availablePANTHERPTHR24314:SF21CHLOROPHYLL(IDE) B REDUCTASE NYC1, CHLOROPLASTIC-RELATEDcoord: 85..517
NoneNo IPR availablePANTHERPTHR24314NON-SPECIFIC LIPID TRANSFER PROTEIN-RELATEDcoord: 85..517
NoneNo IPR availableCDDcd05233SDR_ccoord: 190..420
e-value: 3.98542E-51
score: 172.468
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 188..418

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003821.1MS003821.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity