MS003816 (gene) Bitter gourd (TR) v1

Overview
NameMS003816
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionFlavin-containing monooxygenase
Locationscaffold127: 727118 .. 729100 (+)
RNA-Seq ExpressionMS003816
SyntenyMS003816
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACTATTTGAAAGAAGTGGAAGGCAAAAGACTTCACGATCCTCTGATCCAGAAGAATGGAGAGCCAGCGAGACAAATTAGGGTTCCGGGGCCGCTGATAGTCGGCGCCGGACCGTCGGGACTCGCCGCCGCCGCGTGTCTGAAGCAAAAAGGAATCCCAAGTTTGATCCTTGAGAGAGCCGAGTGCGTAGCTTCGTTGTGGCAGTTCAAGACCTACGATCGCCTCCGCCTTCATCTCCCAAAGCAATTTTGTCAGCTCCCACTTATGCCCTTCCCCTCCAATTTCCCCACTTACCCAACAAAGCAACAATTTTTGGCCTATTTGAGAGCCTACGCCGAGCATTTTGAGTTGAAGCCGGTGTTCAATAGCACGGTGACGGCGGCGGAGTGGGATGAGGGATGTGGGTTTTGGCGGGTCAAGGCTGTTCTTAACTGTGGGAAATTGGGACTCAAGGAAACAACAGAATATGTTTGCAAATGGCTGATTGTGGCAACGGGTGAAAATGCTGAAGAAATTTTTCCACAAATTGAAGGGTTGGAGGAATTTGAGGGCCCCGTTGTTCATACTAGTTCTTACAAGACTGGTGGAGTGTTTCGTGGGAAAAAAGTTTTGGTTATTGGATGTGGGAATTCGGGTATGGAAGTTTGTCTAGACCTCTGCAACTTTAAAGCTTCTCCACATCTCGTCGTTCGCAACTCGGTGAGCATTATCAACGATTTTGTTAATATCTTAATTAGTTGAATGATGTTAAATTGGTTGTCATGGGAAAACTTTTTTGACTTTTTTTTTTTGGCAGGTGCATATTCTACCCCATGAGATGTTGGGAAGATCAACTTTTGGATTGTCGATGTGCTTGCTGAAATGGCTCCCCATGCGCTTAGTGGATAAAATTCTTCTCTTTGTATCACGTTTGGTACTTGGAGACACAGCCAGATTGGGGCTCAATCGCCCGAAACTTGGCCCTCTACAGCTCAAGAATCTGTCTGGAAAAACCCCCGTTTTAGACGTTGGGACTTTGGCAAATATCAGAAGCGGGAAAATTAAGGTTCAATTAATTCTTCTCTGCGATATTCTCCCACAAATTATCAATATTTCTTTCCAAATGTTGTTGATGAGATTTCTAATATATCTGGTCTTCAGGTTCGCCGAGGAGTGACGAGACTAAGCCGCCGCGCCGCCGAGTTCGTCGACGGCAGTGTTGAAAACTTTGACGCTGTCATTTTAGCCACTGGTTATAAAAGTAACGTCCCCTCTTGGCTAAAGGTGCATATTTCCTATTATTTTCTTTAGTTTTGTACTTAAAAAAAAAATTTACTTTTCATTAATCTCTTAATCGAACAAAGTACATTTTGTACGGTGTGTAATGTAAATTGTAATTCATCTCTAGAGTACCATTAAACCAGTTTTCACTGTTTTTATCTTTTAATATCACATCCAACTTGTTTTAATTAGTTGAATTATGCTTGTCATAATTTCAATTTTTAAGCTATACGCATTTGCCTGATAACATGTTAACTATTATACTTCTTTCTCTAATGGTAGATCGATGGACATTAATTGAGTATTAAATGATGGGATTTTTGCTATACATGGTAATAGTTGTCACTTTTAAGTATAGATACATCAACCGAATTAATGAAAATGAATTTGAGACACTATATGTTAAAATTATTCTTTAGTTTCATGACAATTAAGTAATGTTGAAATAAAATATAATGACCCAATAATTAATAATTTGAAGGGTATGATATGATAAATTTTCAGGAAAGGAGTATGTTCAGTGAGAAAGATGGGTTGCCAAGGAAGCAATTTCCAAATGGATGGAAAGGAGAATGTGGATTATACGCAGTTGGGTTCACGAAGAGAGGGCTTCTTGGGGCATCAATGGATGCGAGAAGAACAGCACAAGATATTGAGCTTGTTTGGAAAGCTGAGGACTCGGCTTTAATGCCCTTCACTCACTCTTCTGTATTGCCT

mRNA sequence

ATGGACGACTATTTGAAAGAAGTGGAAGGCAAAAGACTTCACGATCCTCTGATCCAGAAGAATGGAGAGCCAGCGAGACAAATTAGGGTTCCGGGGCCGCTGATAGTCGGCGCCGGACCGTCGGGACTCGCCGCCGCCGCGTGTCTGAAGCAAAAAGGAATCCCAAGTTTGATCCTTGAGAGAGCCGAGTGCGTAGCTTCGTTGTGGCAGTTCAAGACCTACGATCGCCTCCGCCTTCATCTCCCAAAGCAATTTTGTCAGCTCCCACTTATGCCCTTCCCCTCCAATTTCCCCACTTACCCAACAAAGCAACAATTTTTGGCCTATTTGAGAGCCTACGCCGAGCATTTTGAGTTGAAGCCGGTGTTCAATAGCACGGTGACGGCGGCGGAGTGGGATGAGGGATGTGGGTTTTGGCGGGTCAAGGCTGTTCTTAACTGTGGGAAATTGGGACTCAAGGAAACAACAGAATATGTTTGCAAATGGCTGATTGTGGCAACGGGTGAAAATGCTGAAGAAATTTTTCCACAAATTGAAGGGTTGGAGGAATTTGAGGGCCCCGTTGTTCATACTAGTTCTTACAAGACTGGTGGAGTGTTTCGTGGGAAAAAAGTTTTGGTTATTGGATGTGGGAATTCGGGTATGGAAGTTTGTCTAGACCTCTGCAACTTTAAAGCTTCTCCACATCTCGTCGTTCGCAACTCGGTGCATATTCTACCCCATGAGATGTTGGGAAGATCAACTTTTGGATTGTCGATGTGCTTGCTGAAATGGCTCCCCATGCGCTTAGTGGATAAAATTCTTCTCTTTGTATCACGTTTGGTACTTGGAGACACAGCCAGATTGGGGCTCAATCGCCCGAAACTTGGCCCTCTACAGCTCAAGAATCTGTCTGGAAAAACCCCCGTTTTAGACGTTGGGACTTTGGCAAATATCAGAAGCGGGAAAATTAAGGTTCGCCGAGGAGTGACGAGACTAAGCCGCCGCGCCGCCGAGTTCGTCGACGGCAGTGTTGAAAACTTTGACGCTGTCATTTTAGCCACTGGTTATAAAAGTAACGTCCCCTCTTGGCTAAAGGAAAGGAGTATGTTCAGTGAGAAAGATGGGTTGCCAAGGAAGCAATTTCCAAATGGATGGAAAGGAGAATGTGGATTATACGCAGTTGGGTTCACGAAGAGAGGGCTTCTTGGGGCATCAATGGATGCGAGAAGAACAGCACAAGATATTGAGCTTGTTTGGAAAGCTGAGGACTCGGCTTTAATGCCCTTCACTCACTCTTCTGTATTGCCT

Coding sequence (CDS)

ATGGACGACTATTTGAAAGAAGTGGAAGGCAAAAGACTTCACGATCCTCTGATCCAGAAGAATGGAGAGCCAGCGAGACAAATTAGGGTTCCGGGGCCGCTGATAGTCGGCGCCGGACCGTCGGGACTCGCCGCCGCCGCGTGTCTGAAGCAAAAAGGAATCCCAAGTTTGATCCTTGAGAGAGCCGAGTGCGTAGCTTCGTTGTGGCAGTTCAAGACCTACGATCGCCTCCGCCTTCATCTCCCAAAGCAATTTTGTCAGCTCCCACTTATGCCCTTCCCCTCCAATTTCCCCACTTACCCAACAAAGCAACAATTTTTGGCCTATTTGAGAGCCTACGCCGAGCATTTTGAGTTGAAGCCGGTGTTCAATAGCACGGTGACGGCGGCGGAGTGGGATGAGGGATGTGGGTTTTGGCGGGTCAAGGCTGTTCTTAACTGTGGGAAATTGGGACTCAAGGAAACAACAGAATATGTTTGCAAATGGCTGATTGTGGCAACGGGTGAAAATGCTGAAGAAATTTTTCCACAAATTGAAGGGTTGGAGGAATTTGAGGGCCCCGTTGTTCATACTAGTTCTTACAAGACTGGTGGAGTGTTTCGTGGGAAAAAAGTTTTGGTTATTGGATGTGGGAATTCGGGTATGGAAGTTTGTCTAGACCTCTGCAACTTTAAAGCTTCTCCACATCTCGTCGTTCGCAACTCGGTGCATATTCTACCCCATGAGATGTTGGGAAGATCAACTTTTGGATTGTCGATGTGCTTGCTGAAATGGCTCCCCATGCGCTTAGTGGATAAAATTCTTCTCTTTGTATCACGTTTGGTACTTGGAGACACAGCCAGATTGGGGCTCAATCGCCCGAAACTTGGCCCTCTACAGCTCAAGAATCTGTCTGGAAAAACCCCCGTTTTAGACGTTGGGACTTTGGCAAATATCAGAAGCGGGAAAATTAAGGTTCGCCGAGGAGTGACGAGACTAAGCCGCCGCGCCGCCGAGTTCGTCGACGGCAGTGTTGAAAACTTTGACGCTGTCATTTTAGCCACTGGTTATAAAAGTAACGTCCCCTCTTGGCTAAAGGAAAGGAGTATGTTCAGTGAGAAAGATGGGTTGCCAAGGAAGCAATTTCCAAATGGATGGAAAGGAGAATGTGGATTATACGCAGTTGGGTTCACGAAGAGAGGGCTTCTTGGGGCATCAATGGATGCGAGAAGAACAGCACAAGATATTGAGCTTGTTTGGAAAGCTGAGGACTCGGCTTTAATGCCCTTCACTCACTCTTCTGTATTGCCT

Protein sequence

MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSALMPFTHSSVLP
Homology
BLAST of MS003816 vs. NCBI nr
Match: XP_022145028.1 (indole-3-pyruvate monooxygenase YUCCA2-like [Momordica charantia])

HSP 1 Score: 874.8 bits (2259), Expect = 3.1e-250
Identity = 427/430 (99.30%), Postives = 428/430 (99.53%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60
           MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE
Sbjct: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60

Query: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120
           RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK
Sbjct: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120

Query: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180
           PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG
Sbjct: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180

Query: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240
           LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP
Sbjct: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240

Query: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGK 300
           HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVS LVLGDTARLGLNRPKLGPLQLKNLSG+
Sbjct: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSHLVLGDTARLGLNRPKLGPLQLKNLSGR 300

Query: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360
           TPVLDVGTLANIRSGKIKVRRGVTRLSR AAEFVDGSVENFDAVILATGYKSNVPSWLKE
Sbjct: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRCAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360

Query: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420
           RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL
Sbjct: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420

Query: 421 MPFTHSSVLP 431
           MPFTHSSVLP
Sbjct: 421 MPFTHSSVLP 430

BLAST of MS003816 vs. NCBI nr
Match: XP_022961043.1 (indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita moschata])

HSP 1 Score: 745.7 bits (1924), Expect = 2.2e-211
Identity = 361/432 (83.56%), Postives = 392/432 (90.74%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G+P  QI V GPLIVG GPSGLA AACLK KGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGDPVPQIWVQGPLIVGVGPSGLATAACLKHKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+ASLWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIASLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           LKPVFNSTVT AEWDE CGFWRVKAV+      + +  EY+CKWLIVATGENAEE  PQI
Sbjct: 121 LKPVFNSTVTKAEWDERCGFWRVKAVV------VGKCVEYLCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG F GKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFGGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SRL+LGDT+RLGLNRPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRLILGDTSRLGLNRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I+SGKIKVRRGV+RLSR  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKSGKIKVRRGVSRLSRHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE+VWKA++S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVVWKADES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. NCBI nr
Match: XP_023516794.1 (indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.3 bits (1923), Expect = 2.8e-211
Identity = 359/432 (83.10%), Postives = 393/432 (90.97%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G+P  QI V GPLIVG GPSGLA AACLK KGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGDPVPQIWVQGPLIVGVGPSGLATAACLKHKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+ASLWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIASLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           LKPVFNSTVT AEWDE CGFWRVKAV+      + +  EY+CKWLIVATGENAEE  PQI
Sbjct: 121 LKPVFNSTVTKAEWDERCGFWRVKAVV------VGKCVEYLCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG FRGKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFRGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SR++LGDT+RLGL+RPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRMILGDTSRLGLHRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I+SGKIKVRRGV+RLSR  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKSGKIKVRRGVSRLSRHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE++WKA++S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVIWKADES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. NCBI nr
Match: KAG6589910.1 (Indole-3-pyruvate monooxygenase YUCCA6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023580.1 Indole-3-pyruvate monooxygenase YUCCA6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 360/432 (83.33%), Postives = 391/432 (90.51%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G+P  QI V GPLIVG GPSGLA AACLK KGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGDPIPQIWVQGPLIVGVGPSGLATAACLKHKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+ASLWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIASLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           LKPVFNSTVT AEWDE CGFWRVKAV+      + +  EY+CKWLIVATGENAEE  PQI
Sbjct: 121 LKPVFNSTVTKAEWDERCGFWRVKAVV------VGKCVEYLCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG F GKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFGGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SRL+LGDT+RLGLNRPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRLILGDTSRLGLNRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I+SGKIKVRRGV+RLS+  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKSGKIKVRRGVSRLSQHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE+VWKA +S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVVWKANES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. NCBI nr
Match: XP_022987729.1 (indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita maxima])

HSP 1 Score: 738.4 bits (1905), Expect = 3.5e-209
Identity = 357/432 (82.64%), Postives = 389/432 (90.05%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G P  QI V GPLIVG GPSGLA AACLKQKGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGHPVPQIWVQGPLIVGVGPSGLATAACLKQKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+A LWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIAPLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           L PVFNSTVT AEWDE CGFWRVKA +      + +  EY CKWLIVATGENAEE  PQI
Sbjct: 121 LNPVFNSTVTKAEWDERCGFWRVKAFV------VGKCVEYFCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG FRGKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFRGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SRL+LGDT+RLGL+RPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRLILGDTSRLGLHRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I++GKIKVR+GV+RLSR  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKTGKIKVRQGVSRLSRHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE+VWKA++S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVVWKADES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. ExPASy Swiss-Prot
Match: Q9SVQ1 (Indole-3-pyruvate monooxygenase YUCCA2 OS=Arabidopsis thaliana OX=3702 GN=YUC2 PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.3e-162
Identity = 276/409 (67.48%), Postives = 330/409 (80.68%), Query Frame = 0

Query: 3   DYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERA 62
           +++ E  GKR+HDP +    E  R + +PGP+IVG+GPSGLA AACLK + IPSLILER+
Sbjct: 2   EFVTETLGKRIHDPYV----EETRCLMIPGPIIVGSGPSGLATAACLKSRDIPSLILERS 61

Query: 63  ECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPV 122
            C+ASLWQ KTYDRLRLHLPK FC+LPLMPFPS++PTYPTKQQF+ YL +YAEHF+LKPV
Sbjct: 62  TCIASLWQHKTYDRLRLHLPKDFCELPLMPFPSSYPTYPTKQQFVQYLESYAEHFDLKPV 121

Query: 123 FNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLE 182
           FN TV  A++D  CG WRV+     GK    ET EYV +WL+VATGENAEE+ P+I+G+ 
Sbjct: 122 FNQTVEEAKFDRRCGLWRVRT--TGGK--KDETMEYVSRWLVVATGENAEEVMPEIDGIP 181

Query: 183 EFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHE 242
           +F GP++HTSSYK+G +F  KK+LV+GCGNSGMEVCLDLCNF A P LVVR+SVH+LP E
Sbjct: 182 DFGGPILHTSSYKSGEIFSEKKILVVGCGNSGMEVCLDLCNFNALPSLVVRDSVHVLPQE 241

Query: 243 MLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTP 302
           MLG STFG+S  LLKW P+ +VD+ LL +SRLVLGDT RLGL RPKLGPL+ K   GKTP
Sbjct: 242 MLGISTFGISTSLLKWFPVHVVDRFLLRMSRLVLGDTDRLGLVRPKLGPLERKIKCGKTP 301

Query: 303 VLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERS 362
           VLDVGTLA IRSG IKV   + R+   +AEFVDG V+NFDA+ILATGYKSNVP WLK  +
Sbjct: 302 VLDVGTLAKIRSGHIKVYPELKRVMHYSAEFVDGRVDNFDAIILATGYKSNVPMWLKGVN 361

Query: 363 MFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIEL 412
           MFSEKDG P K FPNGWKGE GLYAVGFTK GLLGA++DA++ A+DIE+
Sbjct: 362 MFSEKDGFPHKPFPNGWKGESGLYAVGFTKLGLLGAAIDAKKIAEDIEV 402

BLAST of MS003816 vs. ExPASy Swiss-Prot
Match: Q8VZ59 (Indole-3-pyruvate monooxygenase YUCCA6 OS=Arabidopsis thaliana OX=3702 GN=YUC6 PE=1 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-160
Identity = 272/414 (65.70%), Postives = 325/414 (78.50%), Query Frame = 0

Query: 6   KEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECV 65
           +E+EGK  HD   +    P R   V GP+IVGAGPSGLA AACLK++GI S++LER+ C+
Sbjct: 7   REMEGKLAHDH--RGMTSPRRICVVTGPVIVGAGPSGLATAACLKERGITSVLLERSNCI 66

Query: 66  ASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNS 125
           ASLWQ KTYDRL LHLPKQFC+LP++PFP +FPTYPTKQQF+ YL  YA  F++KP FN 
Sbjct: 67  ASLWQLKTYDRLHLHLPKQFCELPIIPFPGDFPTYPTKQQFIEYLEDYARRFDIKPEFNQ 126

Query: 126 TVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEF- 185
           TV +A +DE  G WRV +V      G + TTEYVC+WL+ ATGENAE + P+ EG+++F 
Sbjct: 127 TVESAAFDENLGMWRVTSV------GEEGTTEYVCRWLVAATGENAEPVVPRFEGMDKFA 186

Query: 186 -EGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEM 245
             G V HT  YKTGG F GK+VLV+GCGNSGMEVCLDLCNF A P LVVR++VH+LP EM
Sbjct: 187 AAGVVKHTCHYKTGGDFAGKRVLVVGCGNSGMEVCLDLCNFGAQPSLVVRDAVHVLPREM 246

Query: 246 LGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPV 305
           LG STFGLSM LLKWLP+RLVD+ LL VSR +LGDT  LGLNRP+LGPL+LKN+SGKTPV
Sbjct: 247 LGTSTFGLSMFLLKWLPIRLVDRFLLVVSRFILGDTTLLGLNRPRLGPLELKNISGKTPV 306

Query: 306 LDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSM 365
           LDVGTLA I++G IKV  G+ RL R   EF +G  E FDA+ILATGYKSNVPSWLKE  M
Sbjct: 307 LDVGTLAKIKTGDIKVCSGIRRLKRHEVEFDNGKTERFDAIILATGYKSNVPSWLKENKM 366

Query: 366 FSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAED 418
           FS+KDG P ++FP GW+GECGLYAVGFTKRG+ GASMDA+R A+DI   WK ++
Sbjct: 367 FSKKDGFPIQEFPEGWRGECGLYAVGFTKRGISGASMDAKRIAEDIHKCWKQDE 412

BLAST of MS003816 vs. ExPASy Swiss-Prot
Match: Q9LFM5 (Probable indole-3-pyruvate monooxygenase YUCCA4 OS=Arabidopsis thaliana OX=3702 GN=YUC4 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.5e-141
Identity = 236/394 (59.90%), Postives = 297/394 (75.38%), Query Frame = 0

Query: 20  KNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRL 79
           +  EP  QI VPGP+IVGAGPSGLA AACL  +G+PS+ILER +C+ASLWQ +TYDRL+L
Sbjct: 5   RESEPT-QIFVPGPIIVGAGPSGLAVAACLSNRGVPSVILERTDCLASLWQKRTYDRLKL 64

Query: 80  HLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFW 139
           HLPK FC+LPLMPFP NFP YP+KQ F++Y+ +YA  F +KPVFN TV  AE+D+  G W
Sbjct: 65  HLPKHFCELPLMPFPKNFPKYPSKQLFISYVESYAARFNIKPVFNQTVEKAEFDDASGLW 124

Query: 140 RVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGV 199
            VK           +   Y   WL+VATGENAE +FP I GL++F GPVVHTS+YK+G  
Sbjct: 125 NVKT----------QDGVYTSTWLVVATGENAEPVFPNIPGLKKFTGPVVHTSAYKSGSA 184

Query: 200 FRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWL 259
           F  +KVLV+GCGNSGMEV LDLC + A PH+VVRNSVH+LP +  G STFG++M LLKW 
Sbjct: 185 FANRKVLVVGCGNSGMEVSLDLCRYNALPHMVVRNSVHVLPRDFFGLSTFGIAMTLLKWF 244

Query: 260 PMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKV 319
           P++LVDK LL ++   LG+T  LGL RPK GP++LKN++GKTPVLDVG ++ IRSG+IKV
Sbjct: 245 PLKLVDKFLLLLANSTLGNTDLLGLRRPKTGPIELKNVTGKTPVLDVGAISLIRSGQIKV 304

Query: 320 RRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGW 379
            + V  ++R  A+F++G    FD++ILATGYKSNVP WLKE S F+ K+G+P+  FPNGW
Sbjct: 305 TQAVKEITRNGAKFLNGKEIEFDSIILATGYKSNVPDWLKENSFFT-KEGMPKTPFPNGW 364

Query: 380 KGECGLYAVGFTKRGLLGASMDARRTAQDIELVW 414
           KGE GLY VGFT+RGL G + DA + A+DI   W
Sbjct: 365 KGEKGLYTVGFTRRGLSGTAYDAVKIAEDITDQW 386

BLAST of MS003816 vs. ExPASy Swiss-Prot
Match: O23024 (Probable indole-3-pyruvate monooxygenase YUCCA3 OS=Arabidopsis thaliana OX=3702 GN=YUC3 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 9.4e-141
Identity = 234/391 (59.85%), Postives = 301/391 (76.98%), Query Frame = 0

Query: 26  RQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRLHLPKQF 85
           R I V GP+IVGAGPSGLA AA LK++G+P +ILERA C+ASLWQ +TYDRL+LHLPKQF
Sbjct: 30  RCIWVNGPVIVGAGPSGLAVAAGLKREGVPFIILERANCIASLWQNRTYDRLKLHLPKQF 89

Query: 86  CQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFWRVKAVL 145
           CQLP  PFP  FP YPTK QF+ YL +YA +F++ P FN TV +A++DE  G WRVK + 
Sbjct: 90  CQLPNYPFPDEFPEYPTKFQFIQYLESYAANFDINPKFNETVQSAKYDETFGLWRVKTIS 149

Query: 146 NCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGVFRGKKV 205
           N G+LG  E  EY+C+W++VATGENAE++ P  EGLE+F G V+H   YK+GG ++GKKV
Sbjct: 150 NMGQLGSCE-FEYICRWIVVATGENAEKVVPDFEGLEDFGGDVLHAGDYKSGGRYQGKKV 209

Query: 206 LVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWLPMRLVD 265
           LV+GCGNSGMEV LDL N  A+P +VVR++VH+LP E+ G+STF L + ++K++P+ L D
Sbjct: 210 LVVGCGNSGMEVSLDLYNHGANPSMVVRSAVHVLPREIFGKSTFELGVTMMKYMPVWLAD 269

Query: 266 KILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKVRRGVTR 325
           K +LF++R++LG+T + GL RPK+GPL+LKN  GKTPVLD+G L  IRSGKIK+  G+ +
Sbjct: 270 KTILFLARIILGNTDKYGLKRPKIGPLELKNKEGKTPVLDIGALPKIRSGKIKIVPGIIK 329

Query: 326 LSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGWKGECGL 385
             +   E +DG V   D+VILATGY+SNVPSWLK+   FS+ DG+P+  FPNGWKGE GL
Sbjct: 330 FGKGKVELIDGRVLEIDSVILATGYRSNVPSWLKDNDFFSD-DGIPKNPFPNGWKGEAGL 389

Query: 386 YAVGFTKRGLLGASMDARRTAQDIELVWKAE 417
           YAVGFT++GL GAS+DA   A DI   WK E
Sbjct: 390 YAVGFTRKGLFGASLDAMSVAHDIANRWKEE 418

BLAST of MS003816 vs. ExPASy Swiss-Prot
Match: O49312 (Probable indole-3-pyruvate monooxygenase YUCCA7 OS=Arabidopsis thaliana OX=3702 GN=YUC7 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 6.3e-137
Identity = 234/391 (59.85%), Postives = 298/391 (76.21%), Query Frame = 0

Query: 26  RQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRLHLPKQF 85
           R I V GP+IVGAGPSGLA AA LK++ +P +ILERA C+ASLWQ +TYDRL+LHLPKQF
Sbjct: 25  RCIWVNGPVIVGAGPSGLAVAADLKRQEVPFVILERANCIASLWQNRTYDRLKLHLPKQF 84

Query: 86  CQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFWRVKAVL 145
           CQLP +PFP + P YPTK QF+ YL +YA HF+L+P FN TV +A++D+  G WRV+ VL
Sbjct: 85  CQLPNLPFPEDIPEYPTKYQFIEYLESYATHFDLRPKFNETVQSAKYDKRFGLWRVQTVL 144

Query: 146 NCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGVFRGKKV 205
               LG  E  EY+C+WL+VATGENAE++ P+ EGLE+F G V+H   YK+G  +RGK+V
Sbjct: 145 RSELLGYCE-FEYICRWLVVATGENAEKVVPEFEGLEDFGGDVLHAGDYKSGERYRGKRV 204

Query: 206 LVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWLPMRLVD 265
           LV+GCGNSGMEV LDLCN  ASP +VVR+SVH+LP E+LG+STF LS+ ++KW+P+ LVD
Sbjct: 205 LVVGCGNSGMEVSLDLCNHDASPSMVVRSSVHVLPREVLGKSTFELSVTMMKWMPVWLVD 264

Query: 266 KILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKVRRGVTR 325
           K LL ++RL+LG+T + GL RP++GPL+LKN +GKTPVLD+G ++ I+SGKIK+  G+ +
Sbjct: 265 KTLLVLTRLLLGNTDKYGLKRPEIGPLELKNTAGKTPVLDIGAISMIKSGKIKIVAGIAK 324

Query: 326 LSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGWKGECGL 385
                 E VDG V   D+VILATGY+SNVPSWLKE  +   + G+ +  FP GWKG+ GL
Sbjct: 325 FGPGKVELVDGRVLQIDSVILATGYRSNVPSWLKENDL--GEIGIEKNPFPKGWKGKAGL 384

Query: 386 YAVGFTKRGLLGASMDARRTAQDIELVWKAE 417
           YAVGFT RGL GAS DA   A DI   WK E
Sbjct: 385 YAVGFTGRGLSGASFDAMSVAHDIANSWKEE 412

BLAST of MS003816 vs. ExPASy TrEMBL
Match: A0A6J1CUU6 (Flavin-containing monooxygenase OS=Momordica charantia OX=3673 GN=LOC111014552 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.5e-250
Identity = 427/430 (99.30%), Postives = 428/430 (99.53%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60
           MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE
Sbjct: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60

Query: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120
           RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK
Sbjct: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120

Query: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180
           PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG
Sbjct: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180

Query: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240
           LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP
Sbjct: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240

Query: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGK 300
           HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVS LVLGDTARLGLNRPKLGPLQLKNLSG+
Sbjct: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSHLVLGDTARLGLNRPKLGPLQLKNLSGR 300

Query: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360
           TPVLDVGTLANIRSGKIKVRRGVTRLSR AAEFVDGSVENFDAVILATGYKSNVPSWLKE
Sbjct: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRCAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360

Query: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420
           RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL
Sbjct: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420

Query: 421 MPFTHSSVLP 431
           MPFTHSSVLP
Sbjct: 421 MPFTHSSVLP 430

BLAST of MS003816 vs. ExPASy TrEMBL
Match: A0A6J1HAR5 (Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461668 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 361/432 (83.56%), Postives = 392/432 (90.74%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G+P  QI V GPLIVG GPSGLA AACLK KGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGDPVPQIWVQGPLIVGVGPSGLATAACLKHKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+ASLWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIASLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           LKPVFNSTVT AEWDE CGFWRVKAV+      + +  EY+CKWLIVATGENAEE  PQI
Sbjct: 121 LKPVFNSTVTKAEWDERCGFWRVKAVV------VGKCVEYLCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG F GKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFGGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SRL+LGDT+RLGLNRPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRLILGDTSRLGLNRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I+SGKIKVRRGV+RLSR  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKSGKIKVRRGVSRLSRHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE+VWKA++S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVVWKADES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. ExPASy TrEMBL
Match: A0A6J1JJP4 (Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485194 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.7e-209
Identity = 357/432 (82.64%), Postives = 389/432 (90.05%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLI--QKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLI 60
           MD Y KEVEGKRLHDPL   QK+G P  QI V GPLIVG GPSGLA AACLKQKGI SLI
Sbjct: 1   MDSYWKEVEGKRLHDPLTQKQKHGHPVPQIWVQGPLIVGVGPSGLATAACLKQKGIQSLI 60

Query: 61  LERAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFE 120
           LERAEC+A LWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPTKQQFLAYL+AYA++F+
Sbjct: 61  LERAECIAPLWQFKTYDRLRLHLPKQFCELPLMPFPSDFPTYPTKQQFLAYLKAYADYFK 120

Query: 121 LKPVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQI 180
           L PVFNSTVT AEWDE CGFWRVKA +      + +  EY CKWLIVATGENAEE  PQI
Sbjct: 121 LNPVFNSTVTKAEWDERCGFWRVKAFV------VGKCVEYFCKWLIVATGENAEEFVPQI 180

Query: 181 EGLEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHI 240
           +GLEEFEGPVVHTSSYKTGG FRGKKVLVIGCGNSGMEVCLDLCNF A PHLVVRNSVHI
Sbjct: 181 DGLEEFEGPVVHTSSYKTGGEFRGKKVLVIGCGNSGMEVCLDLCNFNAFPHLVVRNSVHI 240

Query: 241 LPHEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLS 300
           LPH+MLGRSTFGLSMCLL+WLPMR+VD+ LL +SRL+LGDT+RLGL+RPKLGPLQLKNLS
Sbjct: 241 LPHKMLGRSTFGLSMCLLRWLPMRIVDQFLLLLSRLILGDTSRLGLHRPKLGPLQLKNLS 300

Query: 301 GKTPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWL 360
           GKTPVLDVGT A I++GKIKVR+GV+RLSR  AEFVDGSVE+FDA+ILATGYKSNVPSWL
Sbjct: 301 GKTPVLDVGTFAKIKTGKIKVRQGVSRLSRHTAEFVDGSVESFDAIILATGYKSNVPSWL 360

Query: 361 KERSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDS 420
           KE  MFSEKDG+PRK+FPNGWKGECGLYAVGFTKRGLLGASMDARR A+DIE+VWKA++S
Sbjct: 361 KESKMFSEKDGMPRKEFPNGWKGECGLYAVGFTKRGLLGASMDARRIAEDIEVVWKADES 420

Query: 421 ALMPFTHSSVLP 431
           ALM FTHSSVLP
Sbjct: 421 ALMAFTHSSVLP 426

BLAST of MS003816 vs. ExPASy TrEMBL
Match: A0A6J1I813 (Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472215 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.5e-202
Identity = 345/420 (82.14%), Postives = 378/420 (90.00%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60
           MD+Y KE+EGKR+HDPL QK+G+P  +I V GPLIVGAGPSGLA AACLKQKGI +LILE
Sbjct: 1   MDEYWKELEGKRVHDPLTQKHGDPVPRIWVQGPLIVGAGPSGLATAACLKQKGIQTLILE 60

Query: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120
           RA+C+ASLWQFKTY RLRLHLPKQFC+LPLMPFPS+FPTYPT QQFLAYLR YA +FEL 
Sbjct: 61  RAQCIASLWQFKTYHRLRLHLPKQFCELPLMPFPSHFPTYPTNQQFLAYLRGYANYFELN 120

Query: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180
           PVFNSTVT AEWDE CGFWRVKAV   GKL  +   EY CKWLIVATGENAE+I PQIEG
Sbjct: 121 PVFNSTVTRAEWDERCGFWRVKAV-GEGKLVKETVVEYFCKWLIVATGENAEQIVPQIEG 180

Query: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240
           LEEFEGPV+HTSSYKTGG F GKKVLVIGCGNSG+EVCLDLC+F ASPHLV RNSVHILP
Sbjct: 181 LEEFEGPVIHTSSYKTGGAFGGKKVLVIGCGNSGVEVCLDLCDFNASPHLVARNSVHILP 240

Query: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGK 300
           HEMLGRSTFGLSM LL+WLP+RLVD+ILL +SR +LGDTARLGL+RPKLGPLQLKNLSGK
Sbjct: 241 HEMLGRSTFGLSMSLLRWLPLRLVDQILLILSRFILGDTARLGLSRPKLGPLQLKNLSGK 300

Query: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360
           TPVLDVGT A I++GKIKV  GVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE
Sbjct: 301 TPVLDVGTFAKIKTGKIKVCPGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360

Query: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420
            +MFSEKDG+PRK+  NGWKGECGLYAVGFTKRGLLG SMDARR ++DIELVWKA++S +
Sbjct: 361 SNMFSEKDGMPRKEISNGWKGECGLYAVGFTKRGLLGVSMDARRISEDIELVWKADESQI 419

BLAST of MS003816 vs. ExPASy TrEMBL
Match: A0A6J1EZB9 (Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440935 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 7.1e-200
Identity = 342/420 (81.43%), Postives = 374/420 (89.05%), Query Frame = 0

Query: 1   MDDYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILE 60
           MD+Y KE+EGKR+HDPL QK+G+P  QI V GPLIVGAGPSGLA AACLKQKGI  LILE
Sbjct: 1   MDEYWKELEGKRVHDPLTQKHGDPVPQIWVGGPLIVGAGPSGLATAACLKQKGIQPLILE 60

Query: 61  RAECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELK 120
           RA+C+ASLWQFKTYDRLRLHLPKQFC+LPLMPFPS+FPTYPT QQFLAYLR YA +FEL 
Sbjct: 61  RAQCIASLWQFKTYDRLRLHLPKQFCELPLMPFPSHFPTYPTNQQFLAYLRGYANYFELN 120

Query: 121 PVFNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEG 180
           PVFN+TVT+AEWDE CG WRVKAV   GKL  +   EY CK LIVATGENAE+I PQIEG
Sbjct: 121 PVFNTTVTSAEWDERCGLWRVKAV-GEGKLVKETVVEYFCKCLIVATGENAEQIVPQIEG 180

Query: 181 LEEFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILP 240
           LEEFEGPV+HTSSYKTGG F GKKVLVIGCGNSGMEVCLDLC+F ASPHLV RNSVHILP
Sbjct: 181 LEEFEGPVIHTSSYKTGGAFGGKKVLVIGCGNSGMEVCLDLCDFNASPHLVARNSVHILP 240

Query: 241 HEMLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGK 300
           HEMLGRSTFGLSM LL+WLP+RLVD+ILL +SR +LGDTARLGL+RPKLGPLQLKN+SGK
Sbjct: 241 HEMLGRSTFGLSMSLLRWLPLRLVDQILLILSRFILGDTARLGLSRPKLGPLQLKNMSGK 300

Query: 301 TPVLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360
           TPVLDVGT A I++GKIKV  GVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE
Sbjct: 301 TPVLDVGTFAKIKTGKIKVCPGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKE 360

Query: 361 RSMFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAEDSAL 420
            +MFSEKDG+PRK+  NGWKGECGLYAVGFTKRGLLG SMDA R ++DIEL WKA+ S +
Sbjct: 361 SNMFSEKDGMPRKEISNGWKGECGLYAVGFTKRGLLGVSMDATRISEDIELFWKADQSQI 419

BLAST of MS003816 vs. TAIR 10
Match: AT4G13260.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 276/409 (67.48%), Postives = 330/409 (80.68%), Query Frame = 0

Query: 3   DYLKEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERA 62
           +++ E  GKR+HDP +    E  R + +PGP+IVG+GPSGLA AACLK + IPSLILER+
Sbjct: 2   EFVTETLGKRIHDPYV----EETRCLMIPGPIIVGSGPSGLATAACLKSRDIPSLILERS 61

Query: 63  ECVASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPV 122
            C+ASLWQ KTYDRLRLHLPK FC+LPLMPFPS++PTYPTKQQF+ YL +YAEHF+LKPV
Sbjct: 62  TCIASLWQHKTYDRLRLHLPKDFCELPLMPFPSSYPTYPTKQQFVQYLESYAEHFDLKPV 121

Query: 123 FNSTVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLE 182
           FN TV  A++D  CG WRV+     GK    ET EYV +WL+VATGENAEE+ P+I+G+ 
Sbjct: 122 FNQTVEEAKFDRRCGLWRVRT--TGGK--KDETMEYVSRWLVVATGENAEEVMPEIDGIP 181

Query: 183 EFEGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHE 242
           +F GP++HTSSYK+G +F  KK+LV+GCGNSGMEVCLDLCNF A P LVVR+SVH+LP E
Sbjct: 182 DFGGPILHTSSYKSGEIFSEKKILVVGCGNSGMEVCLDLCNFNALPSLVVRDSVHVLPQE 241

Query: 243 MLGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTP 302
           MLG STFG+S  LLKW P+ +VD+ LL +SRLVLGDT RLGL RPKLGPL+ K   GKTP
Sbjct: 242 MLGISTFGISTSLLKWFPVHVVDRFLLRMSRLVLGDTDRLGLVRPKLGPLERKIKCGKTP 301

Query: 303 VLDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERS 362
           VLDVGTLA IRSG IKV   + R+   +AEFVDG V+NFDA+ILATGYKSNVP WLK  +
Sbjct: 302 VLDVGTLAKIRSGHIKVYPELKRVMHYSAEFVDGRVDNFDAIILATGYKSNVPMWLKGVN 361

Query: 363 MFSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIEL 412
           MFSEKDG P K FPNGWKGE GLYAVGFTK GLLGA++DA++ A+DIE+
Sbjct: 362 MFSEKDGFPHKPFPNGWKGESGLYAVGFTKLGLLGAAIDAKKIAEDIEV 402

BLAST of MS003816 vs. TAIR 10
Match: AT5G25620.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.3e-161
Identity = 272/414 (65.70%), Postives = 325/414 (78.50%), Query Frame = 0

Query: 6   KEVEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECV 65
           +E+EGK  HD   +    P R   V GP+IVGAGPSGLA AACLK++GI S++LER+ C+
Sbjct: 7   REMEGKLAHDH--RGMTSPRRICVVTGPVIVGAGPSGLATAACLKERGITSVLLERSNCI 66

Query: 66  ASLWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNS 125
           ASLWQ KTYDRL LHLPKQFC+LP++PFP +FPTYPTKQQF+ YL  YA  F++KP FN 
Sbjct: 67  ASLWQLKTYDRLHLHLPKQFCELPIIPFPGDFPTYPTKQQFIEYLEDYARRFDIKPEFNQ 126

Query: 126 TVTAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEF- 185
           TV +A +DE  G WRV +V      G + TTEYVC+WL+ ATGENAE + P+ EG+++F 
Sbjct: 127 TVESAAFDENLGMWRVTSV------GEEGTTEYVCRWLVAATGENAEPVVPRFEGMDKFA 186

Query: 186 -EGPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEM 245
             G V HT  YKTGG F GK+VLV+GCGNSGMEVCLDLCNF A P LVVR++VH+LP EM
Sbjct: 187 AAGVVKHTCHYKTGGDFAGKRVLVVGCGNSGMEVCLDLCNFGAQPSLVVRDAVHVLPREM 246

Query: 246 LGRSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPV 305
           LG STFGLSM LLKWLP+RLVD+ LL VSR +LGDT  LGLNRP+LGPL+LKN+SGKTPV
Sbjct: 247 LGTSTFGLSMFLLKWLPIRLVDRFLLVVSRFILGDTTLLGLNRPRLGPLELKNISGKTPV 306

Query: 306 LDVGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSM 365
           LDVGTLA I++G IKV  G+ RL R   EF +G  E FDA+ILATGYKSNVPSWLKE  M
Sbjct: 307 LDVGTLAKIKTGDIKVCSGIRRLKRHEVEFDNGKTERFDAIILATGYKSNVPSWLKENKM 366

Query: 366 FSEKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAED 418
           FS+KDG P ++FP GW+GECGLYAVGFTKRG+ GASMDA+R A+DI   WK ++
Sbjct: 367 FSKKDGFPIQEFPEGWRGECGLYAVGFTKRGISGASMDAKRIAEDIHKCWKQDE 412

BLAST of MS003816 vs. TAIR 10
Match: AT5G25620.2 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 6.4e-161
Identity = 271/412 (65.78%), Postives = 323/412 (78.40%), Query Frame = 0

Query: 8   VEGKRLHDPLIQKNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVAS 67
           +EGK  HD   +    P R   V GP+IVGAGPSGLA AACLK++GI S++LER+ C+AS
Sbjct: 1   MEGKLAHDH--RGMTSPRRICVVTGPVIVGAGPSGLATAACLKERGITSVLLERSNCIAS 60

Query: 68  LWQFKTYDRLRLHLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTV 127
           LWQ KTYDRL LHLPKQFC+LP++PFP +FPTYPTKQQF+ YL  YA  F++KP FN TV
Sbjct: 61  LWQLKTYDRLHLHLPKQFCELPIIPFPGDFPTYPTKQQFIEYLEDYARRFDIKPEFNQTV 120

Query: 128 TAAEWDEGCGFWRVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEF--E 187
            +A +DE  G WRV +V      G + TTEYVC+WL+ ATGENAE + P+ EG+++F   
Sbjct: 121 ESAAFDENLGMWRVTSV------GEEGTTEYVCRWLVAATGENAEPVVPRFEGMDKFAAA 180

Query: 188 GPVVHTSSYKTGGVFRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLG 247
           G V HT  YKTGG F GK+VLV+GCGNSGMEVCLDLCNF A P LVVR++VH+LP EMLG
Sbjct: 181 GVVKHTCHYKTGGDFAGKRVLVVGCGNSGMEVCLDLCNFGAQPSLVVRDAVHVLPREMLG 240

Query: 248 RSTFGLSMCLLKWLPMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLD 307
            STFGLSM LLKWLP+RLVD+ LL VSR +LGDT  LGLNRP+LGPL+LKN+SGKTPVLD
Sbjct: 241 TSTFGLSMFLLKWLPIRLVDRFLLVVSRFILGDTTLLGLNRPRLGPLELKNISGKTPVLD 300

Query: 308 VGTLANIRSGKIKVRRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFS 367
           VGTLA I++G IKV  G+ RL R   EF +G  E FDA+ILATGYKSNVPSWLKE  MFS
Sbjct: 301 VGTLAKIKTGDIKVCSGIRRLKRHEVEFDNGKTERFDAIILATGYKSNVPSWLKENKMFS 360

Query: 368 EKDGLPRKQFPNGWKGECGLYAVGFTKRGLLGASMDARRTAQDIELVWKAED 418
           +KDG P ++FP GW+GECGLYAVGFTKRG+ GASMDA+R A+DI   WK ++
Sbjct: 361 KKDGFPIQEFPEGWRGECGLYAVGFTKRGISGASMDAKRIAEDIHKCWKQDE 404

BLAST of MS003816 vs. TAIR 10
Match: AT5G11320.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 502.3 bits (1292), Expect = 3.9e-142
Identity = 236/394 (59.90%), Postives = 297/394 (75.38%), Query Frame = 0

Query: 20  KNGEPARQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRL 79
           +  EP  QI VPGP+IVGAGPSGLA AACL  +G+PS+ILER +C+ASLWQ +TYDRL+L
Sbjct: 5   RESEPT-QIFVPGPIIVGAGPSGLAVAACLSNRGVPSVILERTDCLASLWQKRTYDRLKL 64

Query: 80  HLPKQFCQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFW 139
           HLPK FC+LPLMPFP NFP YP+KQ F++Y+ +YA  F +KPVFN TV  AE+D+  G W
Sbjct: 65  HLPKHFCELPLMPFPKNFPKYPSKQLFISYVESYAARFNIKPVFNQTVEKAEFDDASGLW 124

Query: 140 RVKAVLNCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGV 199
            VK           +   Y   WL+VATGENAE +FP I GL++F GPVVHTS+YK+G  
Sbjct: 125 NVKT----------QDGVYTSTWLVVATGENAEPVFPNIPGLKKFTGPVVHTSAYKSGSA 184

Query: 200 FRGKKVLVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWL 259
           F  +KVLV+GCGNSGMEV LDLC + A PH+VVRNSVH+LP +  G STFG++M LLKW 
Sbjct: 185 FANRKVLVVGCGNSGMEVSLDLCRYNALPHMVVRNSVHVLPRDFFGLSTFGIAMTLLKWF 244

Query: 260 PMRLVDKILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKV 319
           P++LVDK LL ++   LG+T  LGL RPK GP++LKN++GKTPVLDVG ++ IRSG+IKV
Sbjct: 245 PLKLVDKFLLLLANSTLGNTDLLGLRRPKTGPIELKNVTGKTPVLDVGAISLIRSGQIKV 304

Query: 320 RRGVTRLSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGW 379
            + V  ++R  A+F++G    FD++ILATGYKSNVP WLKE S F+ K+G+P+  FPNGW
Sbjct: 305 TQAVKEITRNGAKFLNGKEIEFDSIILATGYKSNVPDWLKENSFFT-KEGMPKTPFPNGW 364

Query: 380 KGECGLYAVGFTKRGLLGASMDARRTAQDIELVW 414
           KGE GLY VGFT+RGL G + DA + A+DI   W
Sbjct: 365 KGEKGLYTVGFTRRGLSGTAYDAVKIAEDITDQW 386

BLAST of MS003816 vs. TAIR 10
Match: AT1G04610.1 (YUCCA 3 )

HSP 1 Score: 501.5 bits (1290), Expect = 6.7e-142
Identity = 234/391 (59.85%), Postives = 301/391 (76.98%), Query Frame = 0

Query: 26  RQIRVPGPLIVGAGPSGLAAAACLKQKGIPSLILERAECVASLWQFKTYDRLRLHLPKQF 85
           R I V GP+IVGAGPSGLA AA LK++G+P +ILERA C+ASLWQ +TYDRL+LHLPKQF
Sbjct: 30  RCIWVNGPVIVGAGPSGLAVAAGLKREGVPFIILERANCIASLWQNRTYDRLKLHLPKQF 89

Query: 86  CQLPLMPFPSNFPTYPTKQQFLAYLRAYAEHFELKPVFNSTVTAAEWDEGCGFWRVKAVL 145
           CQLP  PFP  FP YPTK QF+ YL +YA +F++ P FN TV +A++DE  G WRVK + 
Sbjct: 90  CQLPNYPFPDEFPEYPTKFQFIQYLESYAANFDINPKFNETVQSAKYDETFGLWRVKTIS 149

Query: 146 NCGKLGLKETTEYVCKWLIVATGENAEEIFPQIEGLEEFEGPVVHTSSYKTGGVFRGKKV 205
           N G+LG  E  EY+C+W++VATGENAE++ P  EGLE+F G V+H   YK+GG ++GKKV
Sbjct: 150 NMGQLGSCE-FEYICRWIVVATGENAEKVVPDFEGLEDFGGDVLHAGDYKSGGRYQGKKV 209

Query: 206 LVIGCGNSGMEVCLDLCNFKASPHLVVRNSVHILPHEMLGRSTFGLSMCLLKWLPMRLVD 265
           LV+GCGNSGMEV LDL N  A+P +VVR++VH+LP E+ G+STF L + ++K++P+ L D
Sbjct: 210 LVVGCGNSGMEVSLDLYNHGANPSMVVRSAVHVLPREIFGKSTFELGVTMMKYMPVWLAD 269

Query: 266 KILLFVSRLVLGDTARLGLNRPKLGPLQLKNLSGKTPVLDVGTLANIRSGKIKVRRGVTR 325
           K +LF++R++LG+T + GL RPK+GPL+LKN  GKTPVLD+G L  IRSGKIK+  G+ +
Sbjct: 270 KTILFLARIILGNTDKYGLKRPKIGPLELKNKEGKTPVLDIGALPKIRSGKIKIVPGIIK 329

Query: 326 LSRRAAEFVDGSVENFDAVILATGYKSNVPSWLKERSMFSEKDGLPRKQFPNGWKGECGL 385
             +   E +DG V   D+VILATGY+SNVPSWLK+   FS+ DG+P+  FPNGWKGE GL
Sbjct: 330 FGKGKVELIDGRVLEIDSVILATGYRSNVPSWLKDNDFFSD-DGIPKNPFPNGWKGEAGL 389

Query: 386 YAVGFTKRGLLGASMDARRTAQDIELVWKAE 417
           YAVGFT++GL GAS+DA   A DI   WK E
Sbjct: 390 YAVGFTRKGLFGASLDAMSVAHDIANRWKEE 418

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145028.13.1e-25099.30indole-3-pyruvate monooxygenase YUCCA2-like [Momordica charantia][more]
XP_022961043.12.2e-21183.56indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita moschata][more]
XP_023516794.12.8e-21183.10indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita pepo subsp. pepo][more]
KAG6589910.11.4e-21083.33Indole-3-pyruvate monooxygenase YUCCA6, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022987729.13.5e-20982.64indole-3-pyruvate monooxygenase YUCCA6-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SVQ13.3e-16267.48Indole-3-pyruvate monooxygenase YUCCA2 OS=Arabidopsis thaliana OX=3702 GN=YUC2 P... [more]
Q8VZ591.8e-16065.70Indole-3-pyruvate monooxygenase YUCCA6 OS=Arabidopsis thaliana OX=3702 GN=YUC6 P... [more]
Q9LFM55.5e-14159.90Probable indole-3-pyruvate monooxygenase YUCCA4 OS=Arabidopsis thaliana OX=3702 ... [more]
O230249.4e-14159.85Probable indole-3-pyruvate monooxygenase YUCCA3 OS=Arabidopsis thaliana OX=3702 ... [more]
O493126.3e-13759.85Probable indole-3-pyruvate monooxygenase YUCCA7 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CUU61.5e-25099.30Flavin-containing monooxygenase OS=Momordica charantia OX=3673 GN=LOC111014552 P... [more]
A0A6J1HAR51.1e-21183.56Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461668 PE... [more]
A0A6J1JJP41.7e-20982.64Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485194 PE=3... [more]
A0A6J1I8131.5e-20282.14Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472215 PE=3... [more]
A0A6J1EZB97.1e-20081.43Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440935 PE... [more]
Match NameE-valueIdentityDescription
AT4G13260.12.4e-16367.48Flavin-binding monooxygenase family protein [more]
AT5G25620.11.3e-16165.70Flavin-binding monooxygenase family protein [more]
AT5G25620.26.4e-16165.78Flavin-binding monooxygenase family protein [more]
AT5G11320.13.9e-14259.90Flavin-binding monooxygenase family protein [more]
AT1G04610.16.7e-14259.85YUCCA 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00469PNDRDTASEIIcoord: 200..224
score: 41.85
coord: 32..54
score: 36.06
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 33..52
score: 37.55
coord: 204..222
score: 36.61
coord: 160..178
score: 25.52
NoneNo IPR availablePIRSRPIRSR000362-1PIRSR000362-1coord: 319..409
e-value: 0.021
score: 11.8
coord: 35..52
e-value: 5.6
score: 3.8
NoneNo IPR availablePANTHERPTHR43539:SF36INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA2coord: 20..423
NoneNo IPR availablePANTHERPTHR43539FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)coord: 20..423
IPR020946Flavin monooxygenase-likePFAMPF00743FMO-likecoord: 35..365
e-value: 1.5E-29
score: 102.8
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 33..417
e-value: 2.9E-79
score: 268.9
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 34..236
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 202..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003816.1MS003816.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0004499 N,N-dimethylaniline monooxygenase activity