MS003796 (gene) Bitter gourd (TR) v1

BLAST of MS003796 vs. NCBI nr
Match: XP_022144907.1 (probable strigolactone esterase DAD2 [Momordica charantia])

HSP 1 Score: 547.0 bits (1408), Expect = 9.4e-152
Identity = 271/272 (99.63%), Postives = 271/272 (99.63%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS
Sbjct: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ
Sbjct: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLCASPRYINTEDYEGGFD SDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF
Sbjct: 121 RLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG
Sbjct: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF
Sbjct: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of MS003796 vs. NCBI nr
Match: XP_022961342.1 (probable strigolactone esterase DAD2 [Cucurbita moschata])

HSP 1 Score: 449.1 bits (1154), Expect = 2.7e-122
Identity = 217/272 (79.78%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRYINTEDYEGGFD+SDIEQ+IAN+E+NYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMEANYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLHEMRPEVAVSLAKTVF  DER +LEKVDIPCTI+ TKTD+  PASVA +MQK+IKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFGVDERGILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. NCBI nr
Match: XP_023515491.1 (probable strigolactone esterase DAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.7 bits (1153), Expect = 3.5e-122
Identity = 217/272 (79.78%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRYINTEDYEGGFD+SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLH+MRPEVAVSLAKTVF  DER++LEKVDIPCTI+ TK DI  PASVA +MQK+IKG
Sbjct: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. NCBI nr
Match: KAG6589894.1 (putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023565.1 putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 447.2 bits (1149), Expect = 1.0e-121
Identity = 216/272 (79.41%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRYINTEDYEGGFD+SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLNRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLH+MRPEV+VSLAKTVF  DER++LEKVDIPC I+ TKTDI  PASVA +MQK+IKG
Sbjct: 181 RKCLHQMRPEVSVSLAKTVFGVDEREILEKVDIPCIILHTKTDIAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. NCBI nr
Match: XP_022987831.1 (probable strigolactone esterase DAD2 [Cucurbita maxima])

HSP 1 Score: 445.7 bits (1145), Expect = 2.9e-121
Identity = 216/272 (79.41%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SY  FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYSAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRY+NTEDYEGGFD+SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYLNTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLIRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLHEMRPEVAVSLAKTVF  DER++LEKVDIPCTI+ TKTD+  PASVA +MQK+IKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-60
Identity = 112/258 (43.41%), Postives = 171/258 (66.28%), Query Frame = 0

Query: 13  ALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPNLFDPQ 72
           ALN R++GSG   +VLAHGFGT+QS W++ILP   + YRVV++D   +G++ +P+ FD +
Sbjct: 8   ALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSV-NPDFFDFR 67

Query: 73  KYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCASPRYI 132
           +Y +   +  DL+ +L+ LG+    ++GHS+S +IG +A  + P LF +LIL+ ASPR++
Sbjct: 68  RYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGASPRFL 127

Query: 133 NTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRFQKCLHEMRPEVA 192
           N EDY GGF++ +IE++ + +E+NYE W   F+ L V    P ++  F + L  MRP++ 
Sbjct: 128 NDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNMRPDIT 187

Query: 193 VSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEVIDTQGH 252
           + +++TVF SD R VL  V +PC I QT  D  VPASVATY++  + G +TV  ++ +GH
Sbjct: 188 LFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWLNIEGH 247

Query: 253 FPHLTAHLQLLQVLGAVL 271
            PHL+A   L Q L   L
Sbjct: 248 LPHLSAPTLLAQELRRAL 264

BLAST of MS003796 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.3e-59
Identity = 109/258 (42.25%), Postives = 170/258 (65.89%), Query Frame = 0

Query: 13  ALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPNLFDPQ 72
           ALN R++G+G   + LAHGFGT+QS W  ILP   Q+YRVV++D   +G++ +P+ FD  
Sbjct: 9   ALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-NPDYFDFN 68

Query: 73  KYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCASPRYI 132
           +Y +   +  DL+ +++ LG+ +  ++GHS+S +IG IA  + P LF +LIL+  SPR++
Sbjct: 69  RYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPRFL 128

Query: 133 NTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRFQKCLHEMRPEVA 192
           N EDY GGF++ +IE++ + +E+NYE W   F+ L V    P ++  F + L  MRP+++
Sbjct: 129 NDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPDIS 188

Query: 193 VSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEVIDTQGH 252
           + +++TVF SD R VL  V +P  ++QT  D+ VPASVA Y++  + G +TVE + T+GH
Sbjct: 189 LFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTEGH 248

Query: 253 FPHLTAHLQLLQVLGAVL 271
            P L+A  QL Q L   L
Sbjct: 249 LPQLSAPAQLAQFLRRAL 265

BLAST of MS003796 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.3e-58
Identity = 109/261 (41.76%), Postives = 171/261 (65.52%), Query Frame = 0

Query: 10  LSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPNLF 69
           L + LN R++GSG   +VL+HGFGT+QS W ++LP L + +RVV++D   +G++ +P+ F
Sbjct: 56  LLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVCAGSV-NPDHF 115

Query: 70  DPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCASP 129
           D ++Y +   +  DL+A+L+ L +    F+GHS+S +IG +A  + P LF +L+L+ ASP
Sbjct: 116 DFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFAKLVLIGASP 175

Query: 130 RYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRFQKCLHEMRP 189
           R++N  DY GGF+  +I+Q+   + +NY  WAT ++ L V    P ++  F + L  MRP
Sbjct: 176 RFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLFNMRP 235

Query: 190 EVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEVIDT 249
           ++++ + +TVF +D R VL  V  PC +VQT  D+ VPASVA Y++  + G +TVE + T
Sbjct: 236 DISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVEFLQT 295

Query: 250 QGHFPHLTAHLQLLQVLGAVL 271
           +GH PHL+A   L QVL   L
Sbjct: 296 EGHLPHLSAPSLLAQVLRRAL 315

BLAST of MS003796 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.2e-58
Identity = 107/259 (41.31%), Postives = 172/259 (66.41%), Query Frame = 0

Query: 13  ALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPNLFDPQ 72
           A N R+ GSG + +V AHGFGT+QS W ++LP   + Y+V+++D   +G++ +P+ FD +
Sbjct: 8   AFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSV-NPDHFDFR 67

Query: 73  KYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCASPRYI 132
           +Y +   +  DL+ +L+ L ++   ++GHS+S + G +A  + P LF +LIL+ ASPR++
Sbjct: 68  RYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPRFL 127

Query: 133 NT-EDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRFQKCLHEMRPEV 192
           N  E+Y GGF++ +IE + + +E+NYE W   F+ L V    P ++  F + L  MRP++
Sbjct: 128 NDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRPDI 187

Query: 193 AVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEVIDTQG 252
           ++ +++TVF SD R +L  V++PC I+QT  D+ VPASVATYM++ I G STV+ +DT+G
Sbjct: 188 SLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDTEG 247

Query: 253 HFPHLTAHLQLLQVLGAVL 271
           H PHL+A   L   L   L
Sbjct: 248 HLPHLSAPSYLAHQLEIAL 265

BLAST of MS003796 vs. ExPASy Swiss-Prot
Match: Q10J20 (Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.3e-57
Identity = 110/261 (42.15%), Postives = 174/261 (66.67%), Query Frame = 0

Query: 8   GLLSRALNARIMGSGAE-AMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDP 67
           G++  A N R++G G    +VLAHGFGT+QS+W  ++P L   YRVV+FD   +G   +P
Sbjct: 2   GIVEEAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGP-TNP 61

Query: 68  NLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLC 127
           + FD  +Y +  G+A DL+A+L+EL ++S I++GHS+S +IG IA    P LF +L+LL 
Sbjct: 62  DYFDFSRYATLEGYALDLLAILQELRVASCIYVGHSVSAVIGAIASISRPDLFSKLVLLS 121

Query: 128 ASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVV-DESDPVSLGRFQKCLH 187
           ASPRY+N  DY GGF++ D++++   + SNY+ W + F+ L V  + + V++  F + L 
Sbjct: 122 ASPRYLNDVDYYGGFEQEDLDELFEAMGSNYKAWCSGFAPLCVGGDMESVAVQEFSRTLF 181

Query: 188 EMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVE 247
            +RP++A+S+A+T+F SD R +L  V +PC IVQ+  D+ VP  V+ Y+ K + G S VE
Sbjct: 182 NIRPDIALSVAQTIFQSDVRSLLPLVTVPCHIVQSTKDLAVPVVVSEYLHKHLGGDSIVE 241

Query: 248 VIDTQGHFPHLTAHLQLLQVL 267
           V+ ++GH P L++   ++ VL
Sbjct: 242 VMPSEGHLPQLSSPDIVIPVL 261

BLAST of MS003796 vs. ExPASy TrEMBL
Match: A0A6J1CV13 (probable strigolactone esterase DAD2 OS=Momordica charantia OX=3673 GN=LOC111014472 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 4.5e-152
Identity = 271/272 (99.63%), Postives = 271/272 (99.63%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS
Sbjct: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ
Sbjct: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLCASPRYINTEDYEGGFD SDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF
Sbjct: 121 RLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG
Sbjct: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF
Sbjct: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of MS003796 vs. ExPASy TrEMBL
Match: A0A6J1H9Y6 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111461858 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.3e-122
Identity = 217/272 (79.78%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRYINTEDYEGGFD+SDIEQ+IAN+E+NYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMEANYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLHEMRPEVAVSLAKTVF  DER +LEKVDIPCTI+ TKTD+  PASVA +MQK+IKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFGVDERGILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. ExPASy TrEMBL
Match: A0A6J1JFG1 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111485258 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.4e-121
Identity = 216/272 (79.41%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNL+DPQKY SY  FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYSAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RLILLC+SPRY+NTEDYEGGFD+SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCSSPRYLNTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLIRL 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +KCLHEMRPEVAVSLAKTVF  DER++LEKVDIPCTI+ TKTD+  PASVA +MQK+IKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of MS003796 vs. ExPASy TrEMBL
Match: A0A6J1KTG9 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111496258 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.6e-120
Identity = 212/272 (77.94%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM++ G  S A+NA+I+GSG+E MVLAHGFG NQSLWDKILPKL  HYRV++FDW+F+
Sbjct: 1   MVVMLQTG-FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDWTFA 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNLFDP KY SY GFA+DLI +L+ELGL+STIF+GHSMSGLIGC+AYT  P LFQ
Sbjct: 61  GSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTIRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RL+LLC+SPRYINTE YEGGF++SDIEQIIA+IESNY+NWAT+F SLVVDE+DPVS+ RF
Sbjct: 121 RLVLLCSSPRYINTEGYEGGFEQSDIEQIIASIESNYQNWATHFPSLVVDEADPVSVSRF 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           QKCLHEMRPEVA+ LAKTVF  DERD+LEKVDIPCTIVQTKTDIVVPASVA +MQ KIKG
Sbjct: 181 QKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNKIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
             TV+VI+T GHFPHLTAHL+LLQVLG +LGF
Sbjct: 241 CCTVQVINTDGHFPHLTAHLELLQVLGTILGF 271

BLAST of MS003796 vs. ExPASy TrEMBL
Match: A0A6J1F5V4 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111441110 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.6e-120
Identity = 212/272 (77.94%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVVM++ G  S A+NA+I+GSG+E MVLAHGFG NQSLWDKILPKL  HYRV++FDW+F+
Sbjct: 1   MVVMLQTG-FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDWTFA 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           G+IKDPNLFDP KY SY GFA+DLI +L+ELGL+STIF+GHSMSGLIGC+AYT+ P LFQ
Sbjct: 61  GSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTKRPDLFQ 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
           RL+LLC+SPRYINTE YEGGF+KSDI+QIIA+IESNY+NWAT+F SLVVDE+DPVS+ RF
Sbjct: 121 RLVLLCSSPRYINTEGYEGGFEKSDIDQIIASIESNYQNWATHFPSLVVDEADPVSVSRF 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           QKCLHEMRPEVA+ LAKTVF  DERD+LE VDIPCTIVQTKTDIVVPASVA +MQ KIKG
Sbjct: 181 QKCLHEMRPEVALPLAKTVFGVDERDILEMVDIPCTIVQTKTDIVVPASVAVFMQNKIKG 240

Query: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 273
             TV+VI+T GHFPHLTAHL+LLQVLG VLGF
Sbjct: 241 CCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of MS003796 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 156/273 (57.14%), Postives = 205/273 (75.09%), Query Frame = 0

Query: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60
           MVV  +   L+ A+NA+I+GSG  +MVLAHGFG +QS+WDKI+P L Q ++V+VFDW FS
Sbjct: 1   MVVNQKISGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFS 60

Query: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120
           GAIKD  L+DP KY S   F+ DLIAL+EEL     +F+GHSMSG+IGC A  + P LF 
Sbjct: 61  GAIKDQTLYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFT 120

Query: 121 RLILLCASPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180
            L+L+ ASPRYIN+EDY+GGF+  DI+ II +I SNYE WA +FSS VVD  D +S+ RF
Sbjct: 121 NLLLIAASPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRF 180

Query: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240
           +K L +M+PE A++LAK VF SDER++L +V +PC ++Q   D+VVP SVA +MQ+KIKG
Sbjct: 181 EKSLKKMKPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKG 240

Query: 241 SSTVEVI-DTQGHFPHLTAHLQLLQVLGAVLGF 273
            STVE+I D  GHFP +T+HL+LL V+  +L F
Sbjct: 241 KSTVEIIEDAIGHFPQMTSHLELLGVMRRLLEF 273

BLAST of MS003796 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.4e-60
Identity = 109/258 (42.25%), Postives = 170/258 (65.89%), Query Frame = 0

Query: 13  ALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPNLFDPQ 72
           ALN R++G+G   + LAHGFGT+QS W  ILP   Q+YRVV++D   +G++ +P+ FD  
Sbjct: 9   ALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-NPDYFDFN 68

Query: 73  KYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCASPRYI 132
           +Y +   +  DL+ +++ LG+ +  ++GHS+S +IG IA  + P LF +LIL+  SPR++
Sbjct: 69  RYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPRFL 128

Query: 133 NTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRFQKCLHEMRPEVA 192
           N EDY GGF++ +IE++ + +E+NYE W   F+ L V    P ++  F + L  MRP+++
Sbjct: 129 NDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPDIS 188

Query: 193 VSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEVIDTQGH 252
           + +++TVF SD R VL  V +P  ++QT  D+ VPASVA Y++  + G +TVE + T+GH
Sbjct: 189 LFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTEGH 248

Query: 253 FPHLTAHLQLLQVLGAVL 271
            P L+A  QL Q L   L
Sbjct: 249 LPQLSAPAQLAQFLRRAL 265

BLAST of MS003796 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 6.4e-58
Identity = 103/260 (39.62%), Postives = 167/260 (64.23%), Query Frame = 0

Query: 8   GLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFSGAIKDPN 67
           G++  A N +++GSG   +VL HGFGT+QS+W  ++P L   YRVV++D +      +P+
Sbjct: 2   GVVEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYD-NMGAGTTNPD 61

Query: 68  LFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQRLILLCA 127
            FD  +Y +  G++ DLIA+LE+L + S IF+GHS+S +IG +A    P LF +++++ A
Sbjct: 62  YFDFDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISA 121

Query: 128 SPRYINTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVV-DESDPVSLGRFQKCLHE 187
           SPRY+N  DY+GGF++ D+ Q+   I SNY+ W   F+ L V  + D +++  F + L  
Sbjct: 122 SPRYVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFN 181

Query: 188 MRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKGSSTVEV 247
           MRP++A+S+ +T+F SD R +L  V +PC I+Q+  D+ VP  V+ Y+   +   S VEV
Sbjct: 182 MRPDIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEV 241

Query: 248 IDTQGHFPHLTAHLQLLQVL 267
           I + GH P L++   ++ V+
Sbjct: 242 IPSDGHLPQLSSPDSVIPVI 260

BLAST of MS003796 vs. TAIR 10
Match: AT1G15490.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 3.7e-05
Identity = 65/275 (23.64%), Postives = 109/275 (39.64%), Query Frame = 0

Query: 21  SGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVV--FD---WSFSGA-----IKDPNLFD 80
           SG   +VL HGFG     W  ++  L Q    VV  FD   W  +       +++  L +
Sbjct: 364 SGQFGVVLVHGFGGGVFSWRHVMGSLAQQLGCVVTAFDRPGWGLTARPHKNDLEERQLLN 423

Query: 81  PQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGC-----IAYTQSP--HLFQRLI 140
           P    S     + LIA   E+G SS +F+GH   GL+       +  T  P   + + ++
Sbjct: 424 PY---SLENQVEMLIAFCYEMGFSSVVFVGHDDGGLLALKAAQRLMATNDPIKVVVKGVV 483

Query: 141 LLCAS------PRY--------INTEDYEGGFDKSDIEQIIANIESNYENWATNFSSLVV 200
           LL  S      P +        +  +       +++I Q++ N  + Y+        L +
Sbjct: 484 LLNTSLSREVVPAFARILLHTSLGKKHLVRPLLRTEIAQVV-NRRAWYDPAKMTTDVLRL 543

Query: 201 DESDPVSLGRFQKCLHE---------MRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQT 256
            ++ P+ +  + + LHE         + P+ A SL K V            ++P  ++  
Sbjct: 544 YKA-PLHVEGWDEALHEIGRLSSEMVLAPQNAASLLKAV-----------ENLPVLVIAG 603