MS003584 (gene) Bitter gourd (TR) v1

Overview
NameMS003584
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNAD(P)H-quinone oxidoreductase subunit T, chloroplastic
Locationscaffold234: 3715040 .. 3715812 (+)
RNA-Seq ExpressionMS003584
SyntenyMS003584
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCCCTCCTATTCCCAATTCTCTGTTCTTGCACAGAAGCTCCTACGTGCCTGCTCGCATTTGGCGGGTCTATGCATCTCAAGCTCCCAACAACCGCCAAAGACCATCACTACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAAGGTTGGATTGGAGGCCCTACGCCTTCCCAATCCAATCGAGACCAGAACAAGCAGGAGAGCTCGGGTCCAAATTTGGCAGAGTTGCTGAATAACTCATCCGATTCTCACTACCAGTACGCTACGTGAGCAGTGGGTTTTGCTTAATTACTTAATTGCAATTTGATTTATGTATGTAGGTGCTGGTGCTGTGCAGGTTCTTAGGAGTAAACGCAGAGGCTGAGCTGGAAGAGATAAAAAGCGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCATCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTGCTGAGCAACGAAGAAAGCCGCAAATTTTACGACTGGACGCTTGCCCAGGAAGAGGCGAGCCGCCAAGCTGAGAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAAGAGTTGGAGAACTTCAAATCCACTCCGGACATGGTGGATCGGCTTGCAGGAAGGAACATGGAGTTGAGTGATCAGGCCACCACTGCTCTTACATTGGATATCTTCATCATTCTCTTTGCTATATGTTGCATTGTATATGTTCTAATTTTTAAAGAGCCATATTAT

mRNA sequence

ATGGCCTCCCCTCCTATTCCCAATTCTCTGTTCTTGCACAGAAGCTCCTACGTGCCTGCTCGCATTTGGCGGGTCTATGCATCTCAAGCTCCCAACAACCGCCAAAGACCATCACTACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAAGGTTGGATTGGAGGCCCTACGCCTTCCCAATCCAATCGAGACCAGAACAAGCAGGAGAGCTCGGGTCCAAATTTGGCAGAGTTGCTGAATAACTCATCCGATTCTCACTACCAGTTCTTAGGAGTAAACGCAGAGGCTGAGCTGGAAGAGATAAAAAGCGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCATCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTGCTGAGCAACGAAGAAAGCCGCAAATTTTACGACTGGACGCTTGCCCAGGAAGAGGCGAGCCGCCAAGCTGAGAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAAGAGTTGGAGAACTTCAAATCCACTCCGGACATGGTGGATCGGCTTGCAGGAAGGAACATGGAGTTGAGTGATCAGGCCACCACTGCTCTTACATTGGATATCTTCATCATTCTCTTTGCTATATGTTGCATTGTATATGTTCTAATTTTTAAAGAGCCATATTAT

Coding sequence (CDS)

ATGGCCTCCCCTCCTATTCCCAATTCTCTGTTCTTGCACAGAAGCTCCTACGTGCCTGCTCGCATTTGGCGGGTCTATGCATCTCAAGCTCCCAACAACCGCCAAAGACCATCACTACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAAGGTTGGATTGGAGGCCCTACGCCTTCCCAATCCAATCGAGACCAGAACAAGCAGGAGAGCTCGGGTCCAAATTTGGCAGAGTTGCTGAATAACTCATCCGATTCTCACTACCAGTTCTTAGGAGTAAACGCAGAGGCTGAGCTGGAAGAGATAAAAAGCGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCATCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTGCTGAGCAACGAAGAAAGCCGCAAATTTTACGACTGGACGCTTGCCCAGGAAGAGGCGAGCCGCCAAGCTGAGAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAAGAGTTGGAGAACTTCAAATCCACTCCGGACATGGTGGATCGGCTTGCAGGAAGGAACATGGAGTTGAGTGATCAGGCCACCACTGCTCTTACATTGGATATCTTCATCATTCTCTTTGCTATATGTTGCATTGTATATGTTCTAATTTTTAAAGAGCCATATTAT

Protein sequence

MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPTPSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY
Homology
BLAST of MS003584 vs. NCBI nr
Match: XP_022152484.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Momordica charantia])

HSP 1 Score: 452.2 bits (1162), Expect = 2.7e-123
Identity = 230/231 (99.57%), Postives = 230/231 (99.57%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPT 60
           MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGP 
Sbjct: 1   MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPM 60

Query: 61  PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT 120
           PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT
Sbjct: 61  PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT 120

Query: 121 SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN 180
           SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN
Sbjct: 121 SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN 180

Query: 181 FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY
Sbjct: 181 FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 231

BLAST of MS003584 vs. NCBI nr
Match: KAG7033427.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.3 bits (978), Expect = 5.8e-102
Identity = 201/240 (83.75%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MASP IPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 244 MASPSIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 303

Query: 61  GPTPSQSNRDQNKQESS------GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRL 120
           GPTPSQS RDQ +Q ++      GP+L +LLNNSSDSHYQFLGV AEA+LEEIKSAYRRL
Sbjct: 304 GPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKSAYRRL 363

Query: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLE 180
           SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLE
Sbjct: 364 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLE 423

Query: 181 DPYEQELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DPYEQEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 424 DPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 482

BLAST of MS003584 vs. NCBI nr
Match: KAG6602740.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 381.3 bits (978), Expect = 5.8e-102
Identity = 201/240 (83.75%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MASP IPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 235 MASPSIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 294

Query: 61  GPTPSQSNRDQNKQESS------GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRL 120
           GPTPSQS RDQ +Q ++      GP+L +LLNNSSDSHYQFLGV AEA+LEEIKSAYRRL
Sbjct: 295 GPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKSAYRRL 354

Query: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLE 180
           SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLE
Sbjct: 355 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLE 414

Query: 181 DPYEQELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DPYEQEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 415 DPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 473

BLAST of MS003584 vs. NCBI nr
Match: XP_022963850.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita moschata])

HSP 1 Score: 381.3 bits (978), Expect = 5.8e-102
Identity = 201/240 (83.75%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MASP IPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 1   MASPSIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 60

Query: 61  GPTPSQSNRDQNKQESS------GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRL 120
           GPTPSQS RDQ +Q ++      GP+L +LLNNSSDSHYQFLGV AEA+LEEIKSAYRRL
Sbjct: 61  GPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKSAYRRL 120

Query: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLE 180
           SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLE
Sbjct: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLE 180

Query: 181 DPYEQELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DPYEQEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 181 DPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 239

BLAST of MS003584 vs. NCBI nr
Match: XP_022990181.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita maxima])

HSP 1 Score: 378.6 bits (971), Expect = 3.8e-101
Identity = 201/236 (85.17%), Postives = 216/236 (91.53%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MAS PIPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 3   MAS-PIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 62

Query: 61  GPTPSQSNRDQNKQESS--GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEY 120
           GPTPSQS RDQ +Q ++  GP+ A+LLNNSSDSHYQFLGV AEA+LEEIKSAYRRLSKEY
Sbjct: 63  GPTPSQSRRDQKEQNNNALGPSFADLLNNSSDSHYQFLGVGAEADLEEIKSAYRRLSKEY 122

Query: 121 HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYE 180
           HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLEDPYE
Sbjct: 123 HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLEDPYE 182

Query: 181 QELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           QEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 183 QELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 236

BLAST of MS003584 vs. ExPASy Swiss-Prot
Match: Q9SMS0 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ndhT PE=1 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 6.9e-74
Identity = 141/212 (66.51%), Postives = 173/212 (81.60%), Query Frame = 0

Query: 21  RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPTPSQSNRDQNKQE-SSGPNLA 80
           R+ R+YASQ P    +PS PGVDTRIHW++ +EGWIGG +    + D++K    S    A
Sbjct: 39  RVTRIYASQGPTKPSKPS-PGVDTRIHWESPDEGWIGGRSDPAKSVDEDKTNLLSDEKFA 98

Query: 81  ELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYE 140
           EL+ +S DSHYQFLGV+ +A+LEEIKSAYRRLSKEYHPDTTSLPLK AS+KFMKL++VY 
Sbjct: 99  ELIKDSFDSHYQFLGVSTDADLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYN 158

Query: 141 VLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELENFKSTPDMVDRLAGRNMELS 200
           VLS+EE+R+FYDWTLAQE ASRQAEK+R+KLEDP EQ+   ++S PDMVDRL GRNMELS
Sbjct: 159 VLSDEETRRFYDWTLAQEVASRQAEKMRMKLEDPKEQDFRGYESIPDMVDRLGGRNMELS 218

Query: 201 DQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DQA TALT DI I+LFA+CCI +V++FK+P Y
Sbjct: 219 DQAMTALTFDILIVLFAVCCIAFVIVFKDPSY 249

BLAST of MS003584 vs. ExPASy Swiss-Prot
Match: Q75VW3 (Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) OX=608538 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 34/63 (53.97%), Postives = 47/63 (74.60%), Query Frame = 0

Query: 89  HYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 148
           +YQ LGV+ +A  EEIK AYRRL+KEYHPD ++   + AS+KF ++ + Y +LS+EE RK
Sbjct: 6   YYQILGVSKDATAEEIKKAYRRLAKEYHPDISA--DENASEKFKEINEAYHILSDEERRK 65

Query: 149 FYD 152
            YD
Sbjct: 66  EYD 66

BLAST of MS003584 vs. ExPASy Swiss-Prot
Match: Q5KWZ8 (Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0

Query: 89  HYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 148
           +Y+ LGV+  A  +EIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS++E R 
Sbjct: 6   YYEILGVSKNATKDEIKKAYRKLSKQYHPDVNKAP--DAAEKFKEIKEAYEVLSDDEKRA 65

Query: 149 FYD 152
            YD
Sbjct: 66  RYD 66

BLAST of MS003584 vs. ExPASy Swiss-Prot
Match: Q45552 (Chaperone protein DnaJ OS=Geobacillus stearothermophilus OX=1422 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.1e-10
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0

Query: 89  HYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 148
           +Y+ LGV+  A  EEIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS+++ R 
Sbjct: 6   YYEILGVSKNATKEEIKKAYRKLSKKYHPDVNKEP--DAAEKFKEIKEAYEVLSDDQKRA 65

Query: 149 FYD 152
            YD
Sbjct: 66  HYD 66

BLAST of MS003584 vs. ExPASy Swiss-Prot
Match: C5D4U0 (Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.4e-10
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0

Query: 89  HYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 148
           +Y+ LGV+  A  EEIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS+++ R 
Sbjct: 6   YYEILGVSKNATKEEIKKAYRKLSKKYHPDINKEP--DAAEKFKEIKEAYEVLSDDQKRA 65

Query: 149 FYD 152
            YD
Sbjct: 66  HYD 66

BLAST of MS003584 vs. ExPASy TrEMBL
Match: A0A6J1DG49 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111020201 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.3e-123
Identity = 230/231 (99.57%), Postives = 230/231 (99.57%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPT 60
           MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGP 
Sbjct: 1   MASPPIPNSLFLHRSSYVPARIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPM 60

Query: 61  PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT 120
           PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT
Sbjct: 61  PSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTT 120

Query: 121 SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN 180
           SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN
Sbjct: 121 SLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELEN 180

Query: 181 FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY
Sbjct: 181 FKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 231

BLAST of MS003584 vs. ExPASy TrEMBL
Match: A0A6J1HJ56 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464036 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.8e-102
Identity = 201/240 (83.75%), Postives = 216/240 (90.00%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MASP IPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 1   MASPSIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 60

Query: 61  GPTPSQSNRDQNKQESS------GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRL 120
           GPTPSQS RDQ +Q ++      GP+L +LLNNSSDSHYQFLGV AEA+LEEIKSAYRRL
Sbjct: 61  GPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKSAYRRL 120

Query: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLE 180
           SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLE
Sbjct: 121 SKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLE 180

Query: 181 DPYEQELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DPYEQEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 181 DPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 239

BLAST of MS003584 vs. ExPASy TrEMBL
Match: A0A6J1JM84 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487152 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.8e-101
Identity = 201/236 (85.17%), Postives = 216/236 (91.53%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MAS PIPNSLFL+R+ + PA   R W VYASQ PNNRQRP  PGVDTRIHWQN+EEGWIG
Sbjct: 3   MAS-PIPNSLFLNRTFHSPARTFRTWPVYASQPPNNRQRPP-PGVDTRIHWQNDEEGWIG 62

Query: 61  GPTPSQSNRDQNKQESS--GPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEY 120
           GPTPSQS RDQ +Q ++  GP+ A+LLNNSSDSHYQFLGV AEA+LEEIKSAYRRLSKEY
Sbjct: 63  GPTPSQSRRDQKEQNNNALGPSFADLLNNSSDSHYQFLGVGAEADLEEIKSAYRRLSKEY 122

Query: 121 HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYE 180
           HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLEDPYE
Sbjct: 123 HPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLEDPYE 182

Query: 181 QELENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           QEL+N+KSTPDMVDRLAGRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 183 QELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKEPYY 236

BLAST of MS003584 vs. ExPASy TrEMBL
Match: A0A5D3BJW7 (NAD(P)H-quinone oxidoreductase subunit T OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002250 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.9e-97
Identity = 187/234 (79.91%), Postives = 204/234 (87.18%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MAS P PNSL  H++ + PA   R WRVYA+QAPNN +R   PGVDTRIHWQNEEEGWIG
Sbjct: 1   MASSPFPNSLLFHKTFHSPAPAFRTWRVYAAQAPNNDRRRPPPGVDTRIHWQNEEEGWIG 60

Query: 61  GPTPSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHP 120
           G     ++++       GP+LA+LLNNSSDSHYQFLGV AEAE+EEIKSAYRRLSKEYHP
Sbjct: 61  GKKKESNDQNNESNNMLGPSLADLLNNSSDSHYQFLGVGAEAEVEEIKSAYRRLSKEYHP 120

Query: 121 DTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQE 180
           DTTSLPLK AS+KFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLEDPYEQE
Sbjct: 121 DTTSLPLKVASEKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLEDPYEQE 180

Query: 181 LENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           L+N++STPDMVDRL GRNMELSDQATTALTLDIFIILFAI CIVYVLIFKEPYY
Sbjct: 181 LQNWQSTPDMVDRLGGRNMELSDQATTALTLDIFIILFAIACIVYVLIFKEPYY 234

BLAST of MS003584 vs. ExPASy TrEMBL
Match: A0A1S3CHS6 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501040 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.9e-97
Identity = 187/234 (79.91%), Postives = 204/234 (87.18%), Query Frame = 0

Query: 1   MASPPIPNSLFLHRSSYVPA---RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIG 60
           MAS P PNSL  H++ + PA   R WRVYA+QAPNN +R   PGVDTRIHWQNEEEGWIG
Sbjct: 1   MASSPFPNSLLFHKTFHSPAPAFRTWRVYAAQAPNNDRRRPPPGVDTRIHWQNEEEGWIG 60

Query: 61  GPTPSQSNRDQNKQESSGPNLAELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHP 120
           G     ++++       GP+LA+LLNNSSDSHYQFLGV AEAE+EEIKSAYRRLSKEYHP
Sbjct: 61  GKKKESNDQNNESNNMLGPSLADLLNNSSDSHYQFLGVGAEAEVEEIKSAYRRLSKEYHP 120

Query: 121 DTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQE 180
           DTTSLPLK AS+KFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA+KLRIKLEDPYEQE
Sbjct: 121 DTTSLPLKVASEKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLEDPYEQE 180

Query: 181 LENFKSTPDMVDRLAGRNMELSDQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           L+N++STPDMVDRL GRNMELSDQATTALTLDIFIILFAI CIVYVLIFKEPYY
Sbjct: 181 LQNWQSTPDMVDRLGGRNMELSDQATTALTLDIFIILFAIACIVYVLIFKEPYY 234

BLAST of MS003584 vs. TAIR 10
Match: AT4G09350.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 4.9e-75
Identity = 141/212 (66.51%), Postives = 173/212 (81.60%), Query Frame = 0

Query: 21  RIWRVYASQAPNNRQRPSLPGVDTRIHWQNEEEGWIGGPTPSQSNRDQNKQE-SSGPNLA 80
           R+ R+YASQ P    +PS PGVDTRIHW++ +EGWIGG +    + D++K    S    A
Sbjct: 39  RVTRIYASQGPTKPSKPS-PGVDTRIHWESPDEGWIGGRSDPAKSVDEDKTNLLSDEKFA 98

Query: 81  ELLNNSSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYE 140
           EL+ +S DSHYQFLGV+ +A+LEEIKSAYRRLSKEYHPDTTSLPLK AS+KFMKL++VY 
Sbjct: 99  ELIKDSFDSHYQFLGVSTDADLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYN 158

Query: 141 VLSNEESRKFYDWTLAQEEASRQAEKLRIKLEDPYEQELENFKSTPDMVDRLAGRNMELS 200
           VLS+EE+R+FYDWTLAQE ASRQAEK+R+KLEDP EQ+   ++S PDMVDRL GRNMELS
Sbjct: 159 VLSDEETRRFYDWTLAQEVASRQAEKMRMKLEDPKEQDFRGYESIPDMVDRLGGRNMELS 218

Query: 201 DQATTALTLDIFIILFAICCIVYVLIFKEPYY 232
           DQA TALT DI I+LFA+CCI +V++FK+P Y
Sbjct: 219 DQAMTALTFDILIVLFAVCCIAFVIVFKDPSY 249

BLAST of MS003584 vs. TAIR 10
Match: AT3G17830.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-11
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 89  HYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 148
           HY  L VN  A L+EIKS+YR+L+++YHPD    P   A DKF ++   YEVLS+EE R 
Sbjct: 64  HYSTLNVNRNATLQEIKSSYRKLARKYHPDMNKNP--GAEDKFKQISAAYEVLSDEEKRS 123

Query: 149 FYD 152
            YD
Sbjct: 124 AYD 124

BLAST of MS003584 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 31/67 (46.27%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 85  SSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 144
           +S  +Y  LGV+  A  +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 145 ESRKFYD 152
           + R  YD
Sbjct: 132 QKRALYD 136

BLAST of MS003584 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 31/67 (46.27%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 85  SSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 144
           +S  +Y  LGV+  A  +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 145 ESRKFYD 152
           + R  YD
Sbjct: 132 QKRALYD 136

BLAST of MS003584 vs. TAIR 10
Match: AT1G80030.2 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 31/67 (46.27%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 85  SSDSHYQFLGVNAEAELEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 144
           +S  +Y  LGV+  A  +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 145 ESRKFYD 152
           + R  YD
Sbjct: 132 QKRALYD 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152484.12.7e-12399.57NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Momordica charantia][more]
KAG7033427.15.8e-10283.75NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita argyrosperma ... [more]
KAG6602740.15.8e-10283.75NAD(P)H-quinone oxidoreductase subunit T, chloroplastic, partial [Cucurbita argy... [more]
XP_022963850.15.8e-10283.75NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita moschata][more]
XP_022990181.13.8e-10185.17NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SMS06.9e-7466.51NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Arabidopsis thaliana ... [more]
Q75VW31.4e-1053.97Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12... [more]
Q5KWZ83.2e-1050.79Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=... [more]
Q455524.1e-1050.79Chaperone protein DnaJ OS=Geobacillus stearothermophilus OX=1422 GN=dnaJ PE=3 SV... [more]
C5D4U05.4e-1050.79Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DG491.3e-12399.57NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Momordica charantia O... [more]
A0A6J1HJ562.8e-10283.75NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita moschata OX... [more]
A0A6J1JM841.8e-10185.17NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita maxima OX=3... [more]
A0A5D3BJW77.9e-9779.91NAD(P)H-quinone oxidoreductase subunit T OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHS67.9e-9779.91NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
AT4G09350.14.9e-7566.51Chaperone DnaJ-domain superfamily protein [more]
AT3G17830.12.3e-1152.38Molecular chaperone Hsp40/DnaJ family protein [more]
AT1G80030.14.2e-1046.27Molecular chaperone Hsp40/DnaJ family protein [more]
AT1G80030.34.2e-1046.27Molecular chaperone Hsp40/DnaJ family protein [more]
AT1G80030.24.2e-1046.27Molecular chaperone Hsp40/DnaJ family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 94..114
NoneNo IPR availableCOILSCoilCoilcoord: 124..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..81
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 108..123
score: 43.45
coord: 90..108
score: 47.68
coord: 126..146
score: 35.13
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 87..146
e-value: 2.7E-17
score: 73.5
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 89..151
e-value: 1.0E-19
score: 70.4
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 88..154
score: 17.95348
IPR001623DnaJ domainCDDcd06257DnaJcoord: 89..143
e-value: 3.40223E-17
score: 71.0373
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 76..173
e-value: 4.5E-21
score: 77.0
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 84..154
IPR044618NAD(P)H-quinone oxidoreductase subunit T-likePANTHERPTHR45283NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT T, CHLOROPLASTICcoord: 6..230
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 131..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003584.1MS003584.1mRNA