MS003557 (gene) Bitter gourd (TR) v1

Overview


Name	MS003557
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Histidine--tRNA ligase
Location	scaffold234: 3548957 .. 3556038 (-)
RNA-Seq Expression	MS003557
Synteny	MS003557

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGACCCTTCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCGTCTATGCCATTGCTCATGGCTTCGCTCTGGTTCGGATCGACTCATCGGCCCTCGAAAGGCTTTCTTCTTCGTCGAACAGCCGAAATGCTGCATCTACCAAGCACCAGGTCTTCATTCCCAATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGCTGTCCTTCTGAACAAGCTCATCATCTCTAGTTCTTCCGGAATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAACTCAAAACCGGAAACTTTACAATTTGAGTCGCTTGACGTGACCGACGAGGAGTTATCCGCGTTCAAACAATCGTGTTATGTTTTGAACGGAATTTGCGCGTTGTTGGACCATCAATCAGCAGCACTATCTTCCATTGCCGATGCTGTGGCTGCCATATCTTGCGAGGCGTCTAGAGCTGACGTGTCTGCGTTTGGTTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCCAATGATATGAAGGTATTGCTCAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTTTCTCGAAGATTCCAAAGAATCATGGATGCTTGAGAGAGCAAGCGAAATTGGTGCACTCGAAAATGCGGGTTGAGTTGAATTCTTGTATGAAATTCGGGAAAGGGGGATCTTTGAGCTCTGGGACAGAGGATTCAGTGCGGACCGCATTGTTGTCATTTGCCGCCGTGCTGTGGGACTTGGGAAAATGTAGTTTGTACAGAGGAAAGTTGATTTTGAGTTCGAACAGCAATGAGAATGTAAAGGCGAGTTTGGTGGGTTTGCTTGATAGGGAATGCCCCAGTAATGAGAGTCTCAAAAAGGAATATAAGTTGGTCTCCGAGTTGGCTCTGGATGACCAGTACGATGAGTTTGTCCATACTGTGAATGTGTTGTTGGTAACTGTATGGAAAATTGTCGCTTGGGAGGCTATAGCAGCTTTTTTGACGATTGAAGGTGGTGAATTGTTAGGAAAGGGTCAAGACGTTGACATAAATGAAGTCAATGAGAAGGTGGTAAAAAAGAATGAGAAGAAAAAGAAAGCAGTCTTGGGTAAAGGGACTAGTGTGGTTGTTCAGTTAATTAAAGATAGACTGCAGGGTAAAGGTGGGGGTGCTTCTGCTGGGGGTCTGGGGTCATTGGAGAACTCAGTAAAAGATCTTTTATCGTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGAAGAAAATCAAAGAGATAGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGTAATGACTTACTTCAGAGCTTTACCTAAATTCCTAGTCATTTAGCCATCCTCCCTTTGTTTTGGTAAGGAAGTGGAATGTCATTAGAAGAATATTATCAGCCTTTGTTTAATGCTGCAACATAGTTTGGATACAATAATAATGAAATTCTACTTAATGAAAATTAGACTTTACCAATTAGTTAGAAAAGTTCGTTTTACCTAATTAGTAACTAATTTTTCATTCGTCTTAATGAAAAGTTCATTTCTTATAAAAAAAATAAAAAATAGAAAAAATAATGAAATTCTACTTTTACTATCAGTTAAAAAATGTTAAATCAATAGACTTTACTAATTAATATTGACATTTTATACATCATTATACCAGTTGAAATTGTTGGAACATGTAGACATGATATACATACTCATAAACCAAACTGCAGAATAGTGATAATTCTAATTTTTTGACTGCGAACTTCTGATATTATCCTTTATTATATTAGTTTAGTTTCAAACCACAGTGGGCAGTAGTTGACGTGTGCATGCTTTAGGTTTTCTATGAAAGGAGAAAAAAAAGGAAAACTCTATTTTTTTTTTAATCACGGTAGCAAGTACATAACATATGTGCTTACTCTTTCTTTTTCTTCCTTTTTTTTGGGTAAGAATCCAACTCTTGTTAAGAATATGTGCGTGCTTTTGGTTTTTCTTTCTCTGTAATCAACTAATCAGTAGTCGTGCCATTCAACTTCATTACAATCTAAAGGGTATTTGTATTGTATATATTATTATTTATGGATAAGACCAGAGGCACATCTATTGAAAGATAACCTGTACTTGTAGAAAAAATTAGCTTTATCTCTTCTGTGCTTAAGTAACATTTCTTTTATTAATATTAATATTTTATGAACAGAGTGGAAAAGCTCTTGTTTCTTTCAGGAATCTTAATCTAGATAAGAAGATAGAAAGTGAGTCATTCTTTCTCATTTGGAAGGATAAAGCTATAGTATTTGCCATTAGCCATTATTTTATTTGTTTATCTATTTCATGCTTTTGGTAACGTTTTTATCTTTTGATTTGATTTGATTTGATTTTACGCCCTCTGTCTATCTTGAGTAAGGTGTTTGTTGTTTTAACTAAATAATGTGTTCTTACGTATTAGGGCACTCGTGATTTTGCGAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGGCATGGAGCCACAGCCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGGCTGATCAGGTTATAATAGATGTTATTACTTGTGTTAACGGACACCACCATTTTCTTTCTTTTCAAGTTTTATAGCTATTAGGGATATTTATCTGAATTTATTTCCCTCCTTTTATTCTAAGCATCTATTAGTGCTTCATGAAATAATTGCATGATTAATGATTAATGAAAATATTTTGATCTGATGCTGAGCATAATGATTAGTTGACTGTTGAAGTTTTTGGAACTTTATTTATCTAATAGTTCTTGTATCTGTTACCTTATTAATGCCATTTACAAAAATTTAACTTTAATTTGTGATGATGAGCTTTTGTGGGCATTTTAACATTCAATCCTTATGTCAAGGTTCTCTAACTTTTTGGGTATCGGGGATTGATATGCGATACATTTTATTTGTTTCTTATTGGATTAATGCTTCTCTGTTTAATCATTGGCTGCATTGCTTTCTCTCTTCTATCTAGCTCTATAGTTTCTCAACAATGAATCCTGGGGCCTTTTCTTTCATTGCCGATGAAGGTTGTGTTTTTTAATCTAAAAAAAATGAATCATGTGGCTTTAAATTTTCTGAACACTGGATAATTTTTATGCGGCAGTGCAATAACTTCTCGTCATTCTTTTTCCCCATCATTGTAATTTGTTTTCCAGTCTGTTTACAGGGAGGGGAGCTCTGTTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTATTACATCATTCAAAAGGTATCAAATAGCAAAAGTTTATAGAAGGGACAACCCATCTAAGGGGAGATTCCGTGAATTTTATCAGTGTGATTTTGACATTGCTGGTCAATATGAAAAGATGGGGCCTGATTTTGAGGTTATTAAGATACTAACGGAGCTACTTGACGAGCTGAGCATTGGAGAGTATGAGGTAACCTTTTTTACTAATTGTTCTGCAGAATATAACTCAATGTTTTTGTTTGCTTGGTGTTTAGTTTGTACAAATTTTTAGTTCTGAATAATAATTATGAATCAGGAAGTATAGCACAAAACATCAAGCAAATTATTAAGCACATTTGCCCTTATTATCATATTCCTACTTCCTAGAGTGGATTGGATTCCTTTTCTATTAGACTATTACACTGAGTTGCCTTAGTCGATTCATGTTGTTATAGCCATTGATTTCTGATGACTCCACTTTGTGGTTAAACCTTTAACCGAATCTATTTTGTATATATTTTCTTCTGCAGATAAAATTGAATCATCGGAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCGCCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAATCTTTTGACCAGATAAAAAGAGAAATGGTAACTAAATTTACTGTTCTAACAATTTTAATTATTAGTCCAGTATGCCTGCAGCTGCCTCTTTCTGCCTTCCACTCTTAGTATCTTTTAGTTATCTGGTTTATGTAATGCTCAATACAGATTGAGTGCGTCAATTTTGAAATTAGGTGGTCTAACATATATTTGCATTTCACTTGAGTCAAAGCTCTTTTCCTTTTTGGTTCTTATCATTTAATTAGTTTTAATTTGATTTGATTTTTTTATTCTGTTAACCTAATTCCCGTTGCGAGGTAAACTCTTACATTGATCTCTTTTCCTAGTTCTTGATTTTTTTGTCTTTCATCCTAGCCAGAATAAGAATGGATTTTTTTAATTGATTATAAATTGAGGTTAACTTGCTAGGTGGAGGAAAAGGGATTGACCGTGGAGATAGCTGAAAAAATTGGTGATTTTGTGAAGGAAAGGGGACATCCATTGGAGTTATTATCTAAGCTTAAACAGGAGCAGTGCCCGTTACTACAAAATAAAGGATCTTTTGATGCATTGGGTGAATTAGAGATTTTATTCAATGCTCTAGAAAAGTCAAAGTGTGTCGACAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTATACTGGTGTAATTTATGAAGCTGTTTTTAAAGGTGGTACCCAGGTAGGCTTCTATATTTTCATCATCAAGTCTGTTAGCACCTTTCTTTCTTTTGACTTGTTATATGCACATGCTGTTGTTTGTTGTTAATTGTGACTCTACTTCAATTGCTTAAAGATTTTCCGTGATAATTCAACTACCAATTATTGGGAGGATTTTGTTGTGCTAGAGCAATCAAAAGAATTCAACTTAATGTTATTTTGAAGAATTGCTAGTAATACAATCTTGCATGGTTAGTTCGAGAGCCTAGCTCCTCCTATCCAAATAATTAACATTTGATATCCCCCTCTGACTTCTTCCACTTTCTTAATAGGTCCCTTGACACGCTCAATAACTCCTAAACCAACAGAATCTATAACTAACTTTTAAAGACAAGAATACCCCCATAAACAGAAATGTCCCTCAGCCCGCAAGGTTCAGAATGTTTAACCCATCTCTTATCCCTGTTTCCCTCTCCTTGTTTATTGAGAATAATGTGTGGCCTAATCAGTATTTGGGCCCAATTAGAAATCTTATCCAGAAGGTTGAAGTGTGGAAATTGCAGTTGGCTAGTTTCTATTCCATTGGCTCCCATGTAGATGTAGCATCAATATTAGGAAGACATTGCCATTGTATTAAAACTTTGTAAAAAATCATTGGGAATGGACTGTCAAATTTGGAAGATTCATTGTTCCACATCCAAAATAAATTCAGACATCAATTGAGGTGGAATAGGGACAGAAGTAGCCAAAGTTTCAATGACATACTTGAATTTGGATACATGAAATATTGGGTGACGTGGGAGAAAGTTGAATTTGGATACAACCTACGAGCCACCTTCCCCACTTTGGCCTAATTGGTGTTGACTCCAGCTTGAGGGTGATGTTGTGCTTCTTTCGTCTCATTGGTAGTAGACCTTGACGGATAGGATTTGGTCTTCACATCCACACAATCCTTCTTGTTGGTGGAGGAACAAACTCTCGAGTACCATCAAGATCCTTACCCTTACTATTTGCTTCTTCTAAGGAATGTTTTCAGTGACAAAAGAGACCCACTAAAACCAGGACCCCATTAAATACACATCTGTATTTCCATACTGAAAACCTATATTTGAAGCCACCCAGTTGACATCCATACTCTGCCTAGAAGCGAGCCATTTCACGCCGAATGACATTGGACCTCCTGTGTGCTGGAGGTAAAAAAAATCCTCAACAAACCTCAAGATGCTGTAGCCCAAGTACAGTACTTTTACAGATTCCATCCTCAAATCTAATGCCTAGTCCGAAACCAACCCCCTACCCTCTTCAATGACATCTGATGGAAATAATTTTGCTCTCTAAGATCACCTTCTCCATGGCTCTATTACCTGCAATGATGTACGAATCTATCGTCATGTTACCGTGCTGCATCCTAATACATATTTTATTTGGACGTTTTAAGGATGTAATTAACAAATCATTTATGTTCTCAGGTTGGTTCTATTGCTGCTGGTGGACGCTATGACAACCTTATTGGAATGTTTGGTTCCAAGCAGGTCCCAGCAGTTGGTGTCAGTCTTGGAATTGAACGAGTTTTTGTTATTATGGAACAACTTTTGAAAGATCAAAACCAGGTAACAACTTAATACTTTTATTTGAATCCTTGGTATAGTGTGAGATGTGAGCTTCTCTTGTAGTTGAATATTAAGATTCGAAGCATTTCGCAACTAATTTTGTTTCTTGGTTCTCTTTATATGGGAACATTTTCACAATTGTATAGAAATTTTGAAAACAATCAAAACAAGTTTCTTCTTTCTTGTTCCCATTTCCACTTTGTTTCAAACATAACTAAAAATATTGTTTTATCATATTTGAATTCATGATATTGGAGCCATGCAATTCATTTTTCTTCATTAAAAATTAAGAAACGGGAACAAGAAACAAAAACGTTACCAAACGAGCCCTAAACTACCTTTGTTAATTGGGGTGCTCTCTTTTCTGAACTCTCGGTGGTTTAGTTCTTAAGACTCCCTCTTATATATTCTGCCATTTAATGATTTCTTTTCTCAATTTTTTTAGTTGTCTTTGTTGAATGGACTATCATCATTTAAATCAACTGTTGGGATTTTGCAGACTACCCGGGCCACTAAGACCGAAGTTTTAGTGAGCATACTGGGGGATGATTTGACACAAGCAGCGGAGCTTGCAAGTGAGATATGGAGTGCAAAACTGAATGCCGAGTTCCTTGTCAATAAAAGAGTAATGAAACATATTGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTTCCAGAAGCAACATCATTGATGAGCTGAAGAAACGTTTGTCCCCT

mRNA sequence

ATGGCAGACCCTTCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCGTCTATGCCATTGCTCATGGCTTCGCTCTGGTTCGGATCGACTCATCGGCCCTCGAAAGGCTTTCTTCTTCGTCGAACAGCCGAAATGCTGCATCTACCAAGCACCAGGTCTTCATTCCCAATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGCTGTCCTTCTGAACAAGCTCATCATCTCTAGTTCTTCCGGAATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAACTCAAAACCGGAAACTTTACAATTTGAGTCGCTTGACGTGACCGACGAGGAGTTATCCGCGTTCAAACAATCGTGTTATGTTTTGAACGGAATTTGCGCGTTGTTGGACCATCAATCAGCAGCACTATCTTCCATTGCCGATGCTGTGGCTGCCATATCTTGCGAGGCGTCTAGAGCTGACGTGTCTGCGTTTGGTTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCCAATGATATGAAGGTATTGCTCAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTTTCTCGAAGATTCCAAAGAATCATGGATGCTTGAGAGAGCAAGCGAAATTGGTGCACTCGAAAATGCGGGTTGAGTTGAATTCTTGTATGAAATTCGGGAAAGGGGGATCTTTGAGCTCTGGGACAGAGGATTCAGTGCGGACCGCATTGTTGTCATTTGCCGCCGTGCTGTGGGACTTGGGAAAATGTAGTTTGTACAGAGGAAAGTTGATTTTGAGTTCGAACAGCAATGAGAATGTAAAGGCGAGTTTGGTGGGTTTGCTTGATAGGGAATGCCCCAGTAATGAGAGTCTCAAAAAGGAATATAAGTTGGTCTCCGAGTTGGCTCTGGATGACCAGTACGATGAGTTTGTCCATACTGTGAATGTGTTGTTGGTAACTGTATGGAAAATTGTCGCTTGGGAGGCTATAGCAGCTTTTTTGACGATTGAAGGTGGTGAATTGTTAGGAAAGGGTCAAGACGTTGACATAAATGAAGTCAATGAGAAGGTGGTAAAAAAGAATGAGAAGAAAAAGAAAGCAGTCTTGGGTAAAGGGACTAGTGTGGTTGTTCAGTTAATTAAAGATAGACTGCAGGGTAAAGGTGGGGGTGCTTCTGCTGGGGGTCTGGGGTCATTGGAGAACTCAGTAAAAGATCTTTTATCGTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGAAGAAAATCAAAGAGATAGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGGCACTCGTGATTTTGCGAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGGCATGGAGCCACAGCCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGGCTGATCAGGGAGGGGAGCTCTGTTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTATTACATCATTCAAAAGGTATCAAATAGCAAAAGTTTATAGAAGGGACAACCCATCTAAGGGGAGATTCCGTGAATTTTATCAGTGTGATTTTGACATTGCTGGTCAATATGAAAAGATGGGGCCTGATTTTGAGGTTATTAAGATACTAACGGAGCTACTTGACGAGCTGAGCATTGGAGAGTATGAGATAAAATTGAATCATCGGAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCGCCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAATCTTTTGACCAGATAAAAAGAGAAATGGTGGAGGAAAAGGGATTGACCGTGGAGATAGCTGAAAAAATTGGTGATTTTGTGAAGGAAAGGGGACATCCATTGGAGTTATTATCTAAGCTTAAACAGGAGCAGTGCCCGTTACTACAAAATAAAGGATCTTTTGATGCATTGGGTGAATTAGAGATTTTATTCAATGCTCTAGAAAAGTCAAAGTGTGTCGACAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTATACTGGTGTAATTTATGAAGCTGTTTTTAAAGGTGGTACCCAGGTTGGTTCTATTGCTGCTGGTGGACGCTATGACAACCTTATTGGAATGTTTGGTTCCAAGCAGGTCCCAGCAGTTGGTGTCAGTCTTGGAATTGAACGAGTTTTTGTTATTATGGAACAACTTTTGAAAGATCAAAACCAGACTACCCGGGCCACTAAGACCGAAGTTTTAGTGAGCATACTGGGGGATGATTTGACACAAGCAGCGGAGCTTGCAAGTGAGATATGGAGTGCAAAACTGAATGCCGAGTTCCTTGTCAATAAAAGAGTAATGAAACATATTGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTTCCAGAAGCAACATCATTGATGAGCTGAAGAAACGTTTGTCCCCT

Coding sequence (CDS)

ATGGCAGACCCTTCTGAGGTCTCCGTGATTACGCTTGGAGGAAAGGGTTCATCGCTTTCATCTAGCTCCGTCTATGCCATTGCTCATGGCTTCGCTCTGGTTCGGATCGACTCATCGGCCCTCGAAAGGCTTTCTTCTTCGTCGAACAGCCGAAATGCTGCATCTACCAAGCACCAGGTCTTCATTCCCAATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGCTGTCCTTCTGAACAAGCTCATCATCTCTAGTTCTTCCGGAATTCGATCGGTTATTCCTGTTCTAATTGCGGAGACACTCAACTCAAAACCGGAAACTTTACAATTTGAGTCGCTTGACGTGACCGACGAGGAGTTATCCGCGTTCAAACAATCGTGTTATGTTTTGAACGGAATTTGCGCGTTGTTGGACCATCAATCAGCAGCACTATCTTCCATTGCCGATGCTGTGGCTGCCATATCTTGCGAGGCGTCTAGAGCTGACGTGTCTGCGTTTGGTTTGATGGACTCGGGTGATGGGTTCGCTTCAAAGGAGGAGGTTGGAGTCGCCAATGATATGAAGGTATTGCTCAATGGATCTAAGCTGGTGGGTAAGATCGAGAGTGGAGCTTTCTCGAAGATTCCAAAGAATCATGGATGCTTGAGAGAGCAAGCGAAATTGGTGCACTCGAAAATGCGGGTTGAGTTGAATTCTTGTATGAAATTCGGGAAAGGGGGATCTTTGAGCTCTGGGACAGAGGATTCAGTGCGGACCGCATTGTTGTCATTTGCCGCCGTGCTGTGGGACTTGGGAAAATGTAGTTTGTACAGAGGAAAGTTGATTTTGAGTTCGAACAGCAATGAGAATGTAAAGGCGAGTTTGGTGGGTTTGCTTGATAGGGAATGCCCCAGTAATGAGAGTCTCAAAAAGGAATATAAGTTGGTCTCCGAGTTGGCTCTGGATGACCAGTACGATGAGTTTGTCCATACTGTGAATGTGTTGTTGGTAACTGTATGGAAAATTGTCGCTTGGGAGGCTATAGCAGCTTTTTTGACGATTGAAGGTGGTGAATTGTTAGGAAAGGGTCAAGACGTTGACATAAATGAAGTCAATGAGAAGGTGGTAAAAAAGAATGAGAAGAAAAAGAAAGCAGTCTTGGGTAAAGGGACTAGTGTGGTTGTTCAGTTAATTAAAGATAGACTGCAGGGTAAAGGTGGGGGTGCTTCTGCTGGGGGTCTGGGGTCATTGGAGAACTCAGTAAAAGATCTTTTATCGTTTTTGGATCCAAAGGCTTCCGAGTTTGATAATCTATTGAAGAAAATCAAAGAGATAGTTGAAAGCAACGAAAGTAGGAGGCTACCAAAGCTTCCCAAGGGCACTCGTGATTTTGCGAAAGAACAAATGGCAATTAGAAAGAAAGCATTTTCAATTATAGAGGGGGTTTTTGAGCGGCATGGAGCCACAGCCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCATGGGGAAATATGGAGAAGATTCCAAGCTGATTTATGATCTGGCTGATCAGGGAGGGGAGCTCTGTTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTATTACATCATTCAAAAGGTATCAAATAGCAAAAGTTTATAGAAGGGACAACCCATCTAAGGGGAGATTCCGTGAATTTTATCAGTGTGATTTTGACATTGCTGGTCAATATGAAAAGATGGGGCCTGATTTTGAGGTTATTAAGATACTAACGGAGCTACTTGACGAGCTGAGCATTGGAGAGTATGAGATAAAATTGAATCATCGGAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCGCCTGAAAAGTTCAGAACCATATGTTCGAGTATTGACAAGTTAGACAAGCAATCTTTTGACCAGATAAAAAGAGAAATGGTGGAGGAAAAGGGATTGACCGTGGAGATAGCTGAAAAAATTGGTGATTTTGTGAAGGAAAGGGGACATCCATTGGAGTTATTATCTAAGCTTAAACAGGAGCAGTGCCCGTTACTACAAAATAAAGGATCTTTTGATGCATTGGGTGAATTAGAGATTTTATTCAATGCTCTAGAAAAGTCAAAGTGTGTCGACAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGTCTTGATTACTATACTGGTGTAATTTATGAAGCTGTTTTTAAAGGTGGTACCCAGGTTGGTTCTATTGCTGCTGGTGGACGCTATGACAACCTTATTGGAATGTTTGGTTCCAAGCAGGTCCCAGCAGTTGGTGTCAGTCTTGGAATTGAACGAGTTTTTGTTATTATGGAACAACTTTTGAAAGATCAAAACCAGACTACCCGGGCCACTAAGACCGAAGTTTTAGTGAGCATACTGGGGGATGATTTGACACAAGCAGCGGAGCTTGCAAGTGAGATATGGAGTGCAAAACTGAATGCCGAGTTCCTTGTCAATAAAAGAGTAATGAAACATATTGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTGGGGGAACGTGAAGTGAGTGAGGGGATTGTGAAGTTGAAAAACGTGGAAACCTTCGAAGAAGTAACAATTTCCAGAAGCAACATCATTGATGAGCTGAAGAAACGTTTGTCCCCT

Protein sequence

MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQVFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEELSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKEEVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKGGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP

Homology

[Close]

BLAST of MS003557 vs. NCBI nr
Match: XP_022152462.1 (histidine--tRNA ligase, cytoplasmic [Momordica charantia])

HSP 1 Score: 1678.3 bits (4345), Expect = 0.0e+00
Identity = 881/884 (99.66%), Postives = 882/884 (99.77%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV
Sbjct: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
           FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIE GAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884

[Close]

BLAST of MS003557 vs. NCBI nr
Match: XP_022954606.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 813/884 (91.97%), Postives = 847/884 (95.81%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IPN+LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLI  SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG         LENSVKD+LS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDL------GLENSVKDILS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. NCBI nr
Match: KAG6602728.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 814/884 (92.08%), Postives = 848/884 (95.93%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG         LENSVKDLLS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDL------GLENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. NCBI nr
Match: XP_023536635.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1552.3 bits (4018), Expect = 0.0e+00
Identity = 811/884 (91.74%), Postives = 845/884 (95.59%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IP++LT EEARASL VLLNKLIISSSSGIRSVIPVLIAETLNS PETLQFESLDVTDEE
Sbjct: 61  VIPDYLTSEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSNPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA+ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVASISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEK V K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG         LENSVKDLLS
Sbjct: 361 DMNRANEKGVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDL------GLENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFRALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. NCBI nr
Match: XP_022990844.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1551.2 bits (4015), Expect = 0.0e+00
Identity = 810/884 (91.63%), Postives = 849/884 (96.04%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GS+SSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEKV+ K+EKKKKAVLGKGTSVVVQLIKDRLQG+     +G LG LENSVKDLLS
Sbjct: 361 DMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGE-----SGDLG-LENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARY+AMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ L
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQHL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. ExPASy Swiss-Prot
Match: F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 1.1e-289
Identity = 547/890 (61.46%), Postives = 669/890 (75.17%), Query Frame = 0


Query: 5   SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQVF-IP 64
           +E S ITLGGKGSSLSSSSVY +A G A VRIDSSA+ER S    +RN  S K   F IP
Sbjct: 3   AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFS----TRNVPSIKRSSFGIP 62

Query: 65  NFLTREEARASLAVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTD 124
             LT EE RASLAVLLNKLI+S     SSS  RSV+P+ I E LN K E+L+   +DVT+
Sbjct: 63  QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122

Query: 125 EELSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFAS 184
            E    ++SC  L GIC+++DH+S  LS I D+VAA+SCE ++AD+++F L+DSGDG   
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182

Query: 185 KEEVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFG 244
           K+ +GVA D+KVLLNG K  GK+E    SKIP  HG  R   K VH+  R ELNS +K G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242

Query: 245 KGGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 304
           K GS ++G  +++ T LL     + +LG CS  R KL      +EN+K     L ++ C 
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKC---LSEKICV 302

Query: 305 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGEL-LGKG 364
            NE+LK  YKL     L++ Y  F H +N  L  VW+IV  EA+AAF  + GGEL + K 
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362

Query: 365 QDVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKD 424
            D D  E ++   KK + +KKAV+GKGTS+V+Q IKDRL      +    + SL    + 
Sbjct: 363 GDAD-KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQ 422

Query: 425 LLSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVF 484
           +L+  +P+   FD+LL K+KEIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ VF
Sbjct: 423 ILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVF 482

Query: 485 ERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGI 544
           +RHGATALDTP FELRETLMGKYGEDSKL+YD+ADQGGELCSLRYDLTVPFARYVAMNGI
Sbjct: 483 KRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGI 542

Query: 545 TSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGE 604
           TSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL IG+
Sbjct: 543 TSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGD 602

Query: 605 YEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEK 664
           YE+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA++
Sbjct: 603 YEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADR 662

Query: 665 IGDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSL 724
           IG+FVKE+G PLELLSKL+QE    L N+ S +AL EL I+F AL++SKC +++VFDLSL
Sbjct: 663 IGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSL 722

Query: 725 ARGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIME 784
           ARGLDYYTGVI+EAV   G +VGSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME
Sbjct: 723 ARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIME 782

Query: 785 QLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAK 844
           +L + Q Q  R T+T+VLVSI+ D+ L +AAEL S++W AK+NAE+LV+KR  KH +RAK
Sbjct: 783 ELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAK 842

Query: 845 ESRIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 883
           ES IPW+V +GE+E+S   V LK +    E  E+ T +R   ++ELKK L
Sbjct: 843 ESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883

[Close]

BLAST of MS003557 vs. ExPASy Swiss-Prot
Match: P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)

HSP 1 Score: 764.2 bits (1972), Expect = 1.6e-219
Identity = 422/760 (55.53%), Postives = 535/760 (70.39%), Query Frame = 0


Query: 138 LDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKEEVGVANDMKVLLNGSKL 197
           +D  ++ L  +ADAVAA+SCEA+R DV+AF +  SGDG ++K+E  VA D+K+LL GSKL
Sbjct: 36  IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95

Query: 198 VGK---IESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKGGSLSS--GTEDSVR 257
           VG     ++ +F+K+P  +G  RE  + +H+++R+ELN+ +K GK  ++ +  G E+++ 
Sbjct: 96  VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155

Query: 258 TALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSNESLKKEYKLVSE 317
                 A  +  + K S+ R +L ++   +  ++  L   ++      + LK    ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLTDGVE-----IDDLK---GMLGK 215

Query: 318 LALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDVDINEV------- 377
           + +D      +  V   L+    I+AWEA  A   IE    + K Q  + NE        
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275

Query: 378 ---NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLSFL 437
               EK     + KKK  LGKGTS V+ L++D L   G    +     L     +L    
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335

Query: 438 DPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGA 497
           DPK     +L+ K+KEIVE+NE RRLPK+PKGTRDF KEQMAIR+ AFSII GVF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395

Query: 498 TALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKR 557
            +LDTP FELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMN I+S KR
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455

Query: 558 YQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEIKL 617
           YQIAKVYRRDNPSKGR+REFYQCDFDIAG YE M PDFEVIK+LTELLD+L IG YEIKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515

Query: 618 NHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFV 677
           NHRKLLDGMLEICGVPPEKFRT+CSSIDKLDKQ+F+Q+K+E+V+EKG++ E A+KIGD V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575

Query: 678 KERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLD 737
           K RG PLE+L +L++E    + N GS  AL ELEILF AL+K+  + K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635

Query: 738 YYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKD 797
           YYTGVIYEAVFKG TQVGSIAAGGRYDNL+GMF  KQVPAVGVSLGIERVF IMEQ   +
Sbjct: 636 YYTGVIYEAVFKGTTQVGSIAAGGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEME 695

Query: 798 QNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRIPW 857
           +NQ  RAT+TEVLVSI+G DL  AAEL SE+W+A + AEF +  R+  H+  A +S IPW
Sbjct: 696 KNQ-IRATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPW 755

Query: 858 IVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL 883
           +V +GE E+S G VKLKN+   +E  + R+     LK++L
Sbjct: 756 MVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQKL 785

[Close]

BLAST of MS003557 vs. ExPASy Swiss-Prot
Match: Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.3e-132
Identity = 243/449 (54.12%), Postives = 330/449 (73.50%), Query Frame = 0


Query: 450 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 509
           K PKGTRD++  QMA+R+K F +I   F+RHGA  +DTP FEL+ETL GKYGEDSKLIYD
Sbjct: 57  KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116

Query: 510 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 569
           L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP  ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 570 DIAGQYEKMGPDFEVIKILTELLDELSIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 629
           DIAGQ++ M PD E +KI+ E+L  L IG++ +K+N R++LDGM  ICGVP  KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236

Query: 630 SIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQNKG 689
           S+DKLDK S++++K EMV EKGL  E+A++IGD+V++ G  + L+ +L Q+   L QNK 
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDP-KLSQNKQ 296

Query: 690 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVF-----KGGTQ---V 749
           + + LG+L++LF  L      DK+ FDLSLARGLDYYTGVIYEAV      + G +   V
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQPPARAGEEPLGV 356

Query: 750 GSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV- 809
           GS+AAGGRYD L+GMF  K  +VP VG+S+G+ER+F I+EQ L+   +  R T+T+VLV 
Sbjct: 357 GSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKVRTTETQVLVA 416

Query: 810 SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRAKESRIPWIVFLGEREVSEG 869
           S     L +  +L SE+W A + AE L   N +++  +   +E+ IP +  +GE+E+ +G
Sbjct: 417 SAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDG 476

Query: 870 IVKLKNVETFEEVTISRSNIIDELKKRLS 884
           ++KL++V + EEV + R ++++E+K+R S
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIKRRTS 503

[Close]

BLAST of MS003557 vs. ExPASy Swiss-Prot
Match: E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)

HSP 1 Score: 471.5 bits (1212), Expect = 2.1e-131
Identity = 246/459 (53.59%), Postives = 332/459 (72.33%), Query Frame = 0


Query: 450 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYD 509
           K  KGTRD+  +QMAIR+K F+II   F+RHGA  +D+P FEL+ETL GKYGEDSKLIYD
Sbjct: 57  KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116

Query: 510 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 569
           L DQGGEL SLRYDLTVPFARY+AMN IT+ KRY IAKVYRRDNP  ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 570 DIAGQYEKMGPDFEVIKILTELLDELSIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 629
           DIAGQY+ M PD E +K++ E+L EL +G++ IK+N R++LDGM  ICGVP EKFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236

Query: 630 SIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQNKG 689
           ++DKLDK +++++K+EMV EKGL+ E+A++I D+V  +G   +L  +L Q+   L Q+K 
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDP-KLSQSKQ 296

Query: 690 SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVF-------------- 749
           +   + ++++LF+ LE  +  DKVVFDLSLARGLDYYTGVIYEA+               
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAILTQANPAPASTPAEQ 356

Query: 750 ----KGGTQVGSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTT 809
                 G  VGS+A GGRYD L+GMF  K  +VP VGVS+GIERVF IMEQ  +  ++  
Sbjct: 357 NGAEDAGVSVGSVAGGGRYDGLVGMFDPKGRKVPCVGVSIGIERVFSIMEQKAELSSEKV 416

Query: 810 RATKTEVLV-SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRAKESRIPWIV 869
           R T+T+VLV S   + L +  +L +E+W+A + AE L   N +++  +   +++ IP + 
Sbjct: 417 RTTETQVLVASAQKNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVA 476

Query: 870 FLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLS 884
            LGE+E+ +G+VKL+NV + EEV + R+ ++DE+KKR S
Sbjct: 477 ILGEQELKDGVVKLRNVASREEVDVPRAELVDEVKKRTS 513

[Close]

BLAST of MS003557 vs. ExPASy Swiss-Prot
Match: P12081 (Histidine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=HARS1 PE=1 SV=2)

HSP 1 Score: 468.8 bits (1205), Expect = 1.4e-130
Identity = 246/466 (52.79%), Postives = 339/466 (72.75%), Query Frame = 0


Query: 433 LKKIKEIVESNESRR--LPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 492
           L K+K  +  +ES++  + K PKGTRD++  QMA+R+K F +I   F+RHGA  +DTP F
Sbjct: 38  LLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVF 97

Query: 493 ELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYR 552
           EL+ETLMGKYGEDSKLIYDL DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYR
Sbjct: 98  ELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYR 157

Query: 553 RDNP--SKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEIKLNHRKLL 612
           RDNP  ++GR+REFYQCDFDIAG ++ M PD E +KI+ E+L  L IG++ +K+N R++L
Sbjct: 158 RDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRIL 217

Query: 613 DGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHP 672
           DGM  ICGV   KFRTICSS+DKLDK S++++K EMV EKGL  E+A++IGD+V++ G  
Sbjct: 218 DGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-G 277

Query: 673 LELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVI 732
           + L+ +L Q+   L QNK + + LG+L++LF  L      DK+ FDLSLARGLDYYTGVI
Sbjct: 278 VSLVEQLLQDP-KLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVI 337

Query: 733 YEAVF-----KGGTQ---VGSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQ 792
           YEAV      + G +   VGS+AAGGRYD L+GMF  K  +VP VG+S+G+ER+F I+EQ
Sbjct: 338 YEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQ 397

Query: 793 LLKDQNQTTRATKTEVLV-SILGDDLTQAAELASEIWSAKLNAEFLV--NKRVMKHIDRA 852
            L+   +  R T+T+VLV S     L +  +L SE+W A + AE L   N +++  +   
Sbjct: 398 RLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYC 457

Query: 853 KESRIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKR 882
           +E+ IP +  +GE+E+ +G++KL++V + EEV + R ++++E+K+R
Sbjct: 458 EEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRR 501

[Close]

BLAST of MS003557 vs. ExPASy TrEMBL
Match: A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)

HSP 1 Score: 1678.3 bits (4345), Expect = 0.0e+00
Identity = 881/884 (99.66%), Postives = 882/884 (99.77%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV
Sbjct: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
           FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIE GAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884

[Close]

BLAST of MS003557 vs. ExPASy TrEMBL
Match: A0A6J1GRC1 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1)

HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 813/884 (91.97%), Postives = 847/884 (95.81%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IPN+LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  VIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GSLSSGTED+VRTALLSFAA+LWDLGKCSL RGKLI  SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEKVV K+EKKKKAVLGKGTSVVVQLIKDRLQGKGG         LENSVKD+LS
Sbjct: 361 DMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDL------GLENSVKDILS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARYVAMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. ExPASy TrEMBL
Match: A0A6J1JT52 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1)

HSP 1 Score: 1551.2 bits (4015), Expect = 0.0e+00
Identity = 810/884 (91.63%), Postives = 849/884 (96.04%), Query Frame = 0


Query: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
           MADP+EVSVITLGGKGSSLSSSS++AIAHGFALVRIDSSALERL+SSSN+RNAAS KHQV
Sbjct: 1   MADPAEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQV 60

Query: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
            IP++LTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE
Sbjct: 61  VIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
           LS FKQSCYVLNG+CALLDHQSAALSSIADAVA ISCEASRADVS F LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
           EVGVANDMKVLLNGSKLVGKIESGA SKIPKNHGCLREQAKLVHSKMRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGKG 240

Query: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
           GS+SSGTED+VRTALLSFAA+LWDLGKCSL RGKLIL SNS++N+KASL GLLDRECPSN
Sbjct: 241 GSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
           ESLKK Y+LVS+LALDD YDEFVHTVNVLL+TVWKIV WEAIA FLTIEGG+L+GKGQDV
Sbjct: 301 ESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQDV 360

Query: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
           D+N  NEKV+ K+EKKKKAVLGKGTSVVVQLIKDRLQG+     +G LG LENSVKDLLS
Sbjct: 361 DMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGE-----SGDLG-LENSVKDLLS 420

Query: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
           FLDPKASEFDNLL KI+EIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH
Sbjct: 421 FLDPKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
           GATALDTPAFELRETLMGKYGEDSKLIYDL+DQGGELCSLRYDLTVPFARY+AMNGITSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEYEI 600
           KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAE+IGD
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGD 660

Query: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
           FVKERGHPLELL+KLKQEQ PLLQNKGS  AL ELEILF+ALEK+KCVDKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ L
Sbjct: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQHL 780

Query: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
           KDQNQTTRATKTE+LVSILGDDLTQAAELASEIWSAKLNAEFL+NKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           PWIVFLGEREVSEGIVKLKNVETFEEVTI RSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878

[Close]

BLAST of MS003557 vs. ExPASy TrEMBL
Match: A0A6J1FE95 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1)

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 797/886 (89.95%), Postives = 844/886 (95.26%), Query Frame = 0


Query: 1   MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
           MADP +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH 
Sbjct: 1   MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60

Query: 61  VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
           +F+P+FLTR+EARASL VLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61  LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120

Query: 121 ELSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
           ELS F+QSCY+LNGICALLDHQSAALSSIADAVA+ISCEASRADVSAF L+DSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180

Query: 181 EEVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
           EEVG+ANDMKV LNGSKLVGKIESGA S IPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240

Query: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
            GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300

Query: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
           S+ESLK+EY+LVSEL LD++Y EFVHTVNVL  TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360

Query: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
           DVDIN  NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG     GGLGSLEN VKDL
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKDL 420

Query: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
           LSFLDPKAS FDNLL+KIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480

Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
           RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540

Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEY 600
           SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+Y
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600

Query: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
           EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660

Query: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
           GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720

Query: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
           RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780

Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
           LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840

Query: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881

[Close]

BLAST of MS003557 vs. ExPASy TrEMBL
Match: A0A6J1JV84 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1)

HSP 1 Score: 1521.9 bits (3939), Expect = 0.0e+00
Identity = 798/886 (90.07%), Postives = 841/886 (94.92%), Query Frame = 0


Query: 1   MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
           MA P +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH 
Sbjct: 1   MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60

Query: 61  VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
           +F+P++LTREEARASL VLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61  LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120

Query: 121 ELSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
           ELS F+QSCYVL+GICALLDHQSAALSSIADAVA+ISCEASRADVSAF LMDSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180

Query: 181 EEVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
           EEVG+ANDMKV LNGSKLVGKIESGA SKIPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240

Query: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
            GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300

Query: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
           SNESLK+EYKLVSEL LD++Y EFVHTVNVL  TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360

Query: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
           DVDIN  NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG     GGLGSLEN VK +
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKAI 420

Query: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
           LSFLDPKAS FDNLLKKIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480

Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
           RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540

Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGEY 600
           SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG+Y
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600

Query: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
           EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660

Query: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
           GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720

Query: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
           RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780

Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
           LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840

Query: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 885
           RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 881

[Close]

BLAST of MS003557 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 997.3 bits (2577), Expect = 7.9e-291
Identity = 547/890 (61.46%), Postives = 669/890 (75.17%), Query Frame = 0


Query: 5   SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQVF-IP 64
           +E S ITLGGKGSSLSSSSVY +A G A VRIDSSA+ER S    +RN  S K   F IP
Sbjct: 3   AERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFS----TRNVPSIKRSSFGIP 62

Query: 65  NFLTREEARASLAVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTD 124
             LT EE RASLAVLLNKLI+S     SSS  RSV+P+ I E LN K E+L+   +DVT+
Sbjct: 63  QGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTE 122

Query: 125 EELSAFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFAS 184
            E    ++SC  L GIC+++DH+S  LS I D+VAA+SCE ++AD+++F L+DSGDG   
Sbjct: 123 GENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGD 182

Query: 185 KEEVGVANDMKVLLNGSKLVGKIESGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFG 244
           K+ +GVA D+KVLLNG K  GK+E    SKIP  HG  R   K VH+  R ELNS +K G
Sbjct: 183 KDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGG 242

Query: 245 KGGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 304
           K GS ++G  +++ T LL     + +LG CS  R KL      +EN+K     L ++ C 
Sbjct: 243 KTGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKC---LSEKICV 302

Query: 305 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGEL-LGKG 364
            NE+LK  YKL     L++ Y  F H +N  L  VW+IV  EA+AAF  + GGEL + K 
Sbjct: 303 ENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKS 362

Query: 365 QDVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKD 424
            D D  E ++   KK + +KKAV+GKGTS+V+Q IKDRL      +    + SL    + 
Sbjct: 363 GDAD-KEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQ 422

Query: 425 LLSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVF 484
           +L+  +P+   FD+LL K+KEIVESNE+RRLPKLPKGTRDFAKEQMA+R+KAFSII+ VF
Sbjct: 423 ILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVF 482

Query: 485 ERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGI 544
           +RHGATALDTP FELRETLMGKYGEDSKL+YD+ADQGGELCSLRYDLTVPFARYVAMNGI
Sbjct: 483 KRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGI 542

Query: 545 TSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGE 604
           TSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL IG+
Sbjct: 543 TSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGD 602

Query: 605 YEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEK 664
           YE+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA++
Sbjct: 603 YEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADR 662

Query: 665 IGDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSL 724
           IG+FVKE+G PLELLSKL+QE    L N+ S +AL EL I+F AL++SKC +++VFDLSL
Sbjct: 663 IGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSL 722

Query: 725 ARGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIME 784
           ARGLDYYTGVI+EAV   G +VGSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME
Sbjct: 723 ARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIME 782

Query: 785 QLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAK 844
           +L + Q Q  R T+T+VLVSI+ D+ L +AAEL S++W AK+NAE+LV+KR  KH +RAK
Sbjct: 783 ELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAK 842

Query: 845 ESRIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 883
           ES IPW+V +GE+E+S   V LK +    E  E+ T +R   ++ELKK L
Sbjct: 843 ESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883

[Close]

BLAST of MS003557 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 157.9 bits (398), Expect = 3.7e-38
Identity = 122/431 (28.31%), Postives = 213/431 (49.42%), Query Frame = 0


Query: 452 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LIYDL 511
           PKGTRDF  E M +R   F+  + V   +G   +D P  E     + K GE+ +  +Y  
Sbjct: 72  PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131

Query: 512 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 571
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ + 
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 191

Query: 572 DIAGQYEKMGPDFEVIKILTELLDELSIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 631
           DI G   ++  + E+I  +      + I   ++  K++ RK+L  +L+  GVP + F  +
Sbjct: 192 DIIG-VPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRV 251

Query: 632 CSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPLLQN 691
           C  IDK++K   D+IK+E+    G++ +  E++          L++LS    +    +  
Sbjct: 252 CIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQL----------LQVLSVKSLDDLEDIIG 311

Query: 692 KGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGSIAAG 751
            G+ +A+ +L+ LF+  EK    + + FD S+ RGL YYTG+++E   + G ++ +I  G
Sbjct: 312 -GAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKG-KLRAICGG 371

Query: 752 GRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLVSILGDDLT- 811
           GRYD L+  +G    PA G   G     VI+E L +         + E +V  L  DL  
Sbjct: 372 GRYDRLLSTYGGDDFPACGFGFGDA---VIVELLKEKDLLPELGQEVENIVCALDKDLQG 431

Query: 812 QAAELASEIWSAKLNAEFLVNKRVMKHI-DRAKESRIPWIVFLGEREVSEGIVKLKNVET 871
            AA +A+ +       + ++  + +K +  RA       +V +G+ E  +G V +K + +
Sbjct: 432 AAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLSS 485

Query: 872 FEEVTISRSNI 875
            E+  +  S++
Sbjct: 492 GEQFQVKLSDL 485

[Close]

BLAST of MS003557 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 1.6e-25
Identity = 115/446 (25.78%), Postives = 200/446 (44.84%), Query Frame = 0


Query: 465  IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDL 524
            +R     I + VF +H A  L+     +   L+    + S+    L   GG++  L Y+L
Sbjct: 796  LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 855

Query: 525  TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 584
             +PF  ++++N  +SFKRY+I+ VYRR       NP         Q DFDI G    +  
Sbjct: 856  RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 915

Query: 585  DFEVIKILTELLDEL-SIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 644
            + EV+K++ ++   +   G  +I LNH  LLD +    G+  E   K   + S +  L  
Sbjct: 916  EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 975

Query: 645  QSFDQ------IKREMVEEKGLTVEIAEKIGDFVKE-RGHPLELLSKLKQEQCPLLQNKG 704
            QS ++      I+R++++E  L   +  ++        G   + L +L+     L  ++ 
Sbjct: 976  QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG---ALRADRP 1035

Query: 705  SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGS------ 764
            +  AL EL  L   L   +  + V  D+ +     Y+  + ++         G+      
Sbjct: 1036 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1095

Query: 765  IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 824
            +A GGRYD L+     ++    +P AVGVSL +E +F   + L  D         T VLV
Sbjct: 1096 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1155

Query: 825  SILGDD--LTQAAELASEIWSAKLNAEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVS 876
               G    L Q  EL +E+W   + AEF+   +  + +  + A E  I  +V + E  V+
Sbjct: 1156 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1215

[Close]

BLAST of MS003557 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 1.6e-25
Identity = 115/446 (25.78%), Postives = 200/446 (44.84%), Query Frame = 0


Query: 465  IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDL 524
            +R     I + VF +H A  L+     +   L+    + S+    L   GG++  L Y+L
Sbjct: 820  LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 879

Query: 525  TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 584
             +PF  ++++N  +SFKRY+I+ VYRR       NP         Q DFDI G    +  
Sbjct: 880  RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 939

Query: 585  DFEVIKILTELLDEL-SIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 644
            + EV+K++ ++   +   G  +I LNH  LLD +    G+  E   K   + S +  L  
Sbjct: 940  EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 999

Query: 645  QSFDQ------IKREMVEEKGLTVEIAEKIGDFVKE-RGHPLELLSKLKQEQCPLLQNKG 704
            QS ++      I+R++++E  L   +  ++        G   + L +L+     L  ++ 
Sbjct: 1000 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG---ALRADRP 1059

Query: 705  SFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQVGS------ 764
            +  AL EL  L   L   +  + V  D+ +     Y+  + ++         G+      
Sbjct: 1060 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1119

Query: 765  IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 824
            +A GGRYD L+     ++    +P AVGVSL +E +F   + L  D         T VLV
Sbjct: 1120 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1179

Query: 825  SILGDD--LTQAAELASEIWSAKLNAEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVS 876
               G    L Q  EL +E+W   + AEF+   +  + +  + A E  I  +V + E  V+
Sbjct: 1180 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1239

[Close]

BLAST of MS003557 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 6.2e-17
Identity = 58/187 (31.02%), Postives = 92/187 (49.20%), Query Frame = 0


Query: 452 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LIYDL 511
           PKGTRDF  E M +R   F+  + V    G   +D P  E     + K GE+ +  +Y  
Sbjct: 72  PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131

Query: 512 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 571
            D+G    +LR +LT   AR V   G +     K + + + +R +  ++GR RE YQ + 
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNM 191

Query: 572 DIAGQYEKMGPDFEVIKILTELLDELSIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 631
           DI G  E +  + E+I  +      + I   ++  K++ RK+L   L+  GVP + F  +
Sbjct: 192 DIIGVPE-VTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRV 251

Query: 632 CSSIDKL 633
           C  IDK+
Sbjct: 252 CFIIDKV 257

The following BLAST results are available for this feature:

BLAST of MS003557 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152462.1	0.0e+00	99.66	histidine--tRNA ligase, cytoplasmic [Momordica charantia]	[more]
XP_022954606.1	0.0e+00	91.97	histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >XP_022954615.1 hi...	[more]
KAG6602728.1	0.0e+00	92.08	Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023536635.1	0.0e+00	91.74	histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022990844.1	0.0e+00	91.63	histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima]	[more]

BLAST of MS003557 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IYF8	1.1e-289	61.46	Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760...	[more]
P93422	1.6e-219	55.53	Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q2KI84	3.3e-132	54.12	Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1	[more]
E9QI36	2.1e-131	53.59	Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2	[more]
P12081	1.4e-130	52.79	Histidine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=HARS1 PE=1 SV=2	[more]

BLAST of MS003557 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGB6	0.0e+00	99.66	Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1	[more]
A0A6J1GRC1	0.0e+00	91.97	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1	[more]
A0A6J1JT52	0.0e+00	91.63	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1	[more]
A0A6J1FE95	0.0e+00	89.95	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1	[more]
A0A6J1JV84	0.0e+00	90.07	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1	[more]

BLAST of MS003557 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02760.1	7.9e-291	61.46	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G46100.1	3.7e-38	28.31	Histidyl-tRNA synthetase 1	[more]
AT3G59410.1	1.6e-25	25.78	protein kinase family protein	[more]
AT3G59410.2	1.6e-25	25.78	protein kinase family protein	[more]
AT5G03406.1	6.2e-17	31.02	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 438..786 e-value: 1.9E-142 score: 476.3
None	No IPR available	GENE3D	1.20.200.10	Fumarase/aspartase (Central domain)	coord: 129..305 e-value: 1.2E-5 score: 26.5
None	No IPR available	PANTHER	PTHR11476:SF7	HISTIDYL-TRNA SYNTHETASE	coord: 131..883
None	No IPR available	PANTHER	PTHR11476	HISTIDYL-TRNA SYNTHETASE	coord: 131..883
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 450..776
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 792..882
IPR015807	Histidine-tRNA ligase	TIGRFAM	TIGR00442	TIGR00442	coord: 451..870 e-value: 4.1E-99 score: 330.2
IPR015807	Histidine-tRNA ligase	HAMAP	MF_00127	His_tRNA_synth	coord: 447..880 score: 23.908701
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 788..883 e-value: 4.8E-20 score: 73.2
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	PFAM	PF13393	tRNA-synt_His	coord: 454..772 e-value: 5.2E-43 score: 147.5
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	CDD	cd00773	HisRS-like_core	coord: 462..777 e-value: 8.29482E-101 score: 312.615
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 803..880 e-value: 3.0E-12 score: 46.5
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 430..776 score: 9.489691
IPR033656	Histidyl-anticodon-binding	CDD	cd00859	HisRS_anticodon	coord: 792..879 e-value: 9.92186E-20 score: 82.5876
IPR008948	L-Aspartase-like	SUPERFAMILY	48557	L-aspartase-like	coord: 15..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003557.1	MS003557.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004821	histidine-tRNA ligase activity