MS003542 (gene) Bitter gourd (TR) v1

Overview


Name	MS003542
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	5'-3' exonuclease
Location	scaffold234: 3428907 .. 3431574 (+)
RNA-Seq Expression	MS003542
Synteny	MS003542

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAGGCGGGCGCCAACATAGGCGTGAATATTCCGCCATTTCTGAACTCTGCATCGCGTAGTTCCTTACCCTCCAGAACTCTGAAACCAGAATCGACATTAACAACGAAATCCAATTCATGGAGAACGAAGGCGTTGCAGCTAAGTGCCTTTCCGGTAGCATCTCATTCTACCTTTAAACAAACAGATGGCGGGACGTTGCAGCCAATAATTGAAGCGGATAATCCAAGAAATGGAAGGGTCTTTTTCCTGGACGTAAATCCATTATGCTACGAAGGTAGCAGACCTAGTTTGCACAATTTTGGTCGCTGGGCTTCCATATTCTTCGAGCAAGTTAGCCACAGTGATCCTGTTATTGCTGTAAGTGCCATTGTTTACTATCAATAATGTCGAGCGCATAGGTTGTTTTTTTGCTCATTTTTTTTATATACGTTACTGATTTCGGGCTTAATTTTACTCATGCTTGTGGTTTCAATCCATGTCTGAACTTATTAATTTCTCTCAGTCTGTCTGTTTTGTTGGGTTATTGTATGAATTTATCTGTTTAATCGCATGCTCCCATTTTTGTGCTATCGTATGGGACAATTCCAATTCTACAAGTTATCTCGTTCGGCTAATTTCTAAGCTCTCAGTGACTAAAACAATCTCATATTCCGTTCTCTCTTTTAGGTTTCTACTTCTTTTCCAAATTCTAGCAATTTCAAATTCTGGAACGTCAAAGCGCCCACAAGGTTTATTTATTTATGAAAGGGATAATTAGTGGGAATTACAAAACCAACAGTTAATTGAAAACAAAATATGACTCCCTCACCCTGCCTGCCTCCAGCTGTAGTGTATTTAGTGCAGGAAGGGAGGTAAAAACTTCGATCCCATTTTCTTTCATGGCGTTTTGGGCTCAATGCTTACTGCAGTGAGCAAATGTTAAGATTACACTGTCACCTATTGGGCCTAGTGCTTACTGAAGAAGTTTTCGAGGAGGAGATTTTTATTTCTCTCAAGTGTAAATGCTTGTAGGAATATAGTGATTTGACATTATTTTGTGGTCTTTCGTTTGAATAAGCTTAGTTTGTAACTTGGGAGATGGATGTGCACAACCACGAACGGGGTTGGAATATGAATCTATGATCTGCTTATCGGTATTTCTTTTGTGCATCAGTTTCTCATAACCTTAGGCACCCCTTCCCAGTAGCCTGTGTAAGTTGTAACTAAGGTATTATCCCGTTTTCTTATTTGTGTTGTCACATAGCTGGATATTTGGCTTCGGGCGAGATTTCTTTCTTTGGACGAATTTCTCTTAATGAGGAAAACGTTATCAGAAACAGTAGTATGCATCATTTCTCTAACTCTTCAAATTCGTACTGGTTCCGCAGGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTATTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACAATCATCTTCACAAAGATATACTAAGGGAAATTCTAGAAGGCCATATCAAGTAATAAGAGATGCTCTCAGAAACTGTAATGTGCCAGTAGGTGAACTTTGTCTAAATAATTTTCGACGTATTTGCTTGGGACTTATTGATGGTGTAGACTAGCTAATGTAAATTGCAGAAAGTTTATTGGTCTATTATCCATGATCAGAATTGTGGCGTTGGAATATTAAACTTTGAAAGAATGAATGTTCAGAACTGTTAAGATTTGTCCTTTTCATTCTATAAAGCTATGTCATGCAGGTTGTAAAGGTTGACGGTCATGAAGCAGATGATGTTGTAGCTACACTTGTGGACCAAGTTTTACAAAGAGGGTTTCGGGTGGTTATAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTCGTGATGCCTTTGCCAGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTATAACTGCGATCCATGCTCTGACTTGAGTCTGAGTAAGTACTCGGTCTGCAAACGTAGAATATAGAAACCACAAAGAGAAATCAGAAAATTTTACCATTTTCTTATCACGAGGAATTATAAGTCCTTCAAAATATATACATGTGCAATCGTTGTTCTGGAGATTTAACCAGAAGGAAGTATGATAAATGTTGAAGTATGATATAGTGATTTTCTTCATAGGATGTATTATGGGTGATGAGGTAGATGGCGTTCCGGGAATCCAGCATGTTGCTCCTGGATTTGGTCGAAAAACTGCAATGAAGCTCTTGAAGAAACATGGTTCTTTGGAGAATCTACTCAGTGCTGCTGCAATAAGAACTGTGGGCAGACCGTATGCACAAGATGCACTTACAAAGTATGCCGATTACCTGCGTATGAATTATAAAGTTCTAGCCCTGAGAAGGTGATTCCTTTCCCTTCATTTTCTTCCCACCTAATCTTATGCTGGTGCAATGTGTAGTTTGTTTGTGCATCTTTACTCTTGGAATAACTTGAATTGTAGCTATTTCTTTCTTGACTAGATTTTTTTGGCTTTGGGTCAAATTGTTGTACAGAGATGTTGATGTTCAATTTCAAGAGGAGTGGTTGGTTGAAAGAGACAGACAAAACGATTCGGCTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTCACTTGTTCAACCATCAAAACGGGTC

mRNA sequence

ATGGCTGAGGCGGGCGCCAACATAGGCGTGAATATTCCGCCATTTCTGAACTCTGCATCGCGTAGTTCCTTACCCTCCAGAACTCTGAAACCAGAATCGACATTAACAACGAAATCCAATTCATGGAGAACGAAGGCGTTGCAGCTAAGTGCCTTTCCGGTAGCATCTCATTCTACCTTTAAACAAACAGATGGCGGGACGTTGCAGCCAATAATTGAAGCGGATAATCCAAGAAATGGAAGGGTCTTTTTCCTGGACGTAAATCCATTATGCTACGAAGGTAGCAGACCTAGTTTGCACAATTTTGGTCGCTGGGCTTCCATATTCTTCGAGCAAGTTAGCCACAGTGATCCTGTTATTGCTGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTATTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACAATCATCTTCACAAAGATATACTAAGGGAAATTCTAGAAGGCCATATCAAGTAATAAGAGATGCTCTCAGAAACTGTAATGTGCCAGTTGTAAAGGTTGACGGTCATGAAGCAGATGATGTTGTAGCTACACTTGTGGACCAAGTTTTACAAAGAGGGTTTCGGGTGGTTATAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTCGTGATGCCTTTGCCAGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTATAACTGCGATCCATGCTCTGACTTGAGTCTGAGATGTATTATGGGTGATGAGGTAGATGGCGTTCCGGGAATCCAGCATGTTGCTCCTGGATTTGGTCGAAAAACTGCAATGAAGCTCTTGAAGAAACATGGTTCTTTGGAGAATCTACTCAGTGCTGCTGCAATAAGAACTGTGGGCAGACCGTATGCACAAGATGCACTTACAAAGTATGCCGATTACCTGCGTATGAATTATAAAGTTCTAGCCCTGAGAAGAGATGTTGATGTTCAATTTCAAGAGGAGTGGTTGGTTGAAAGAGACAGACAAAACGATTCGGCTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTCACTTGTTCAACCATCAAAACGGGTC

Coding sequence (CDS)

ATGGCTGAGGCGGGCGCCAACATAGGCGTGAATATTCCGCCATTTCTGAACTCTGCATCGCGTAGTTCCTTACCCTCCAGAACTCTGAAACCAGAATCGACATTAACAACGAAATCCAATTCATGGAGAACGAAGGCGTTGCAGCTAAGTGCCTTTCCGGTAGCATCTCATTCTACCTTTAAACAAACAGATGGCGGGACGTTGCAGCCAATAATTGAAGCGGATAATCCAAGAAATGGAAGGGTCTTTTTCCTGGACGTAAATCCATTATGCTACGAAGGTAGCAGACCTAGTTTGCACAATTTTGGTCGCTGGGCTTCCATATTCTTCGAGCAAGTTAGCCACAGTGATCCTGTTATTGCTGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTATTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACAATCATCTTCACAAAGATATACTAAGGGAAATTCTAGAAGGCCATATCAAGTAATAAGAGATGCTCTCAGAAACTGTAATGTGCCAGTTGTAAAGGTTGACGGTCATGAAGCAGATGATGTTGTAGCTACACTTGTGGACCAAGTTTTACAAAGAGGGTTTCGGGTGGTTATAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTCGTGATGCCTTTGCCAGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTATAACTGCGATCCATGCTCTGACTTGAGTCTGAGATGTATTATGGGTGATGAGGTAGATGGCGTTCCGGGAATCCAGCATGTTGCTCCTGGATTTGGTCGAAAAACTGCAATGAAGCTCTTGAAGAAACATGGTTCTTTGGAGAATCTACTCAGTGCTGCTGCAATAAGAACTGTGGGCAGACCGTATGCACAAGATGCACTTACAAAGTATGCCGATTACCTGCGTATGAATTATAAAGTTCTAGCCCTGAGAAGAGATGTTGATGTTCAATTTCAAGAGGAGTGGTTGGTTGAAAGAGACAGACAAAACGATTCGGCTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTCACTTGTTCAACCATCAAAACGGGTC

Protein sequence

MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTFKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENNDRNSLVQPSKRV

Homology

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BLAST of MS003542 vs. NCBI nr
Match: XP_022152532.1 (uncharacterized protein LOC111020233 [Momordica charantia])

HSP 1 Score: 745.0 bits (1922), Expect = 3.2e-211
Identity = 370/372 (99.46%), Postives = 370/372 (99.46%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF 60
           MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF
Sbjct: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF 60

Query: 61  KQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI 120
           KQTDGGTLQPIIEADNPR GRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI
Sbjct: 61  KQTDGGTLQPIIEADNPRKGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI 120

Query: 121 AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK 180
           AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK
Sbjct: 121 AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK 180

Query: 181 VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH 240
           VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH
Sbjct: 181 VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH 240

Query: 241 YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI 300
           YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI
Sbjct: 241 YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI 300

Query: 301 RTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN 360
           RTVGRPYAQDALTKYADYLR NYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN
Sbjct: 301 RTVGRPYAQDALTKYADYLRTNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN 360

Query: 361 DRNSLVQPSKRV 373
           DRNSLVQPSKRV
Sbjct: 361 DRNSLVQPSKRV 372

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BLAST of MS003542 vs. NCBI nr
Match: XP_038884032.1 (5'-3' exonuclease [Benincasa hispida])

HSP 1 Score: 649.0 bits (1673), Expect = 2.4e-182
Identity = 326/378 (86.24%), Postives = 348/378 (92.06%), Query Frame = 0


Query: 1   MAEAGANIGV-NIPPFLNSASRSSLPSRTLKPESTLTT--KSNSWRTKALQLSAFPVASH 60
           MAEA ANIGV N+PPFLNS+SR+SLPSRTLK E+ +T+  K+N+WRTK L+L+ F  +S 
Sbjct: 1   MAEASANIGVLNLPPFLNSSSRTSLPSRTLKAEAAVTSKLKANTWRTKPLKLTVFAASSR 60

Query: 61  ST---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVS 120
            T   F QTD G  QP IEADNPRNGRVFFLDVNPLCY+G+RPSLHNFGRW SIFFE+VS
Sbjct: 61  YTSAAFNQTDCGKFQPRIEADNPRNGRVFFLDVNPLCYQGNRPSLHNFGRWLSIFFEEVS 120

Query: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNC 180
           HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQ SSQR+TKGNSRR YQVIRDALRNC
Sbjct: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQPSSQRFTKGNSRRSYQVIRDALRNC 180

Query: 181 NVPVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWS 240
           NVPVVKVDG EADDVVATLV+QVLQRG RVVIASPDKDFKQLISEDVQLVMPLPELNRWS
Sbjct: 181 NVPVVKVDGQEADDVVATLVEQVLQRGVRVVIASPDKDFKQLISEDVQLVMPLPELNRWS 240

Query: 241 FYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENL 300
           FYTLRHYLAQY+CDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENL
Sbjct: 241 FYTLRHYLAQYHCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL 300

Query: 301 LSAAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILS 360
           LSAAAIRTVG+PYAQ ALTKYA+YLR NYKVLALRRDVDVQFQ+EWLVERDRQND AILS
Sbjct: 301 LSAAAIRTVGKPYAQVALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRQNDLAILS 360

Query: 361 KFVENNDRNSLVQPSKRV 373
           KFVEN +RNSL QPSKRV
Sbjct: 361 KFVENTERNSLAQPSKRV 378

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BLAST of MS003542 vs. NCBI nr
Match: XP_022990137.1 (uncharacterized protein LOC111487120 [Cucurbita maxima])

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-182
Identity = 323/371 (87.06%), Postives = 341/371 (91.91%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLK-PESTLTTKSNSWRTKALQLSAFPVASHST 60
           MAEA ANIG+NIPPFLNSAS +SLPSRTLK  ES  TTK+ SWRTK L+LS F  AS ST
Sbjct: 1   MAEASANIGLNIPPFLNSASPTSLPSRTLKAAESVRTTKAKSWRTKPLKLSVFAAASRST 60

Query: 61  ---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHS 120
              FKQ + G L P +EADNPR GRVFFLDVNPLCY+GSRPSLHNFGRW SIFFE+VSHS
Sbjct: 61  SSGFKQAEDGKLPPRMEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVSHS 120

Query: 121 DPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNV 180
           DPVIAV DGEGGSEHRRLLLPSYKAHRIKFTRQSSSQR+TKGNS R YQVIRDALR+C+V
Sbjct: 121 DPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSCDV 180

Query: 181 PVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240
           PV+K+ GHEADDVVATLV+QVLQRGFR VIASPDKDFKQLISEDVQLVMPLPELNRWSFY
Sbjct: 181 PVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240

Query: 241 TLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300
           TL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS
Sbjct: 241 TLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300

Query: 301 AAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKF 360
           AAAIRTVG+PYAQDALTKYADYLR NYKVLALRRDVDVQF+EEWLVERDRQNDS ILSKF
Sbjct: 301 AAAIRTVGKPYAQDALTKYADYLRTNYKVLALRRDVDVQFREEWLVERDRQNDSTILSKF 360

Query: 361 VENNDRNSLVQ 368
           VENNDRNSL +
Sbjct: 361 VENNDRNSLAE 371

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BLAST of MS003542 vs. NCBI nr
Match: KAG7033404.1 (hypothetical protein SDJN02_07460 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.8 bits (1662), Expect = 4.5e-181
Identity = 318/371 (85.71%), Postives = 339/371 (91.37%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLK-PESTLTTKSNSWRTKALQLSAFPVASHST 60
           MAEA ANIG+NIPPFLNS S +SLPSRTLK  ES  TTK+  WRTK L+LS FP +S ST
Sbjct: 1   MAEASANIGLNIPPFLNSTSPTSLPSRTLKAAESVRTTKAKPWRTKPLKLSVFPASSRST 60

Query: 61  ---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHS 120
              FKQ + G L P +EADNPR GRVFFLDVNPLCY+GSRPSLHNFGRW SIFFE+VS S
Sbjct: 61  SSGFKQAEDGKLAPTVEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVSRS 120

Query: 121 DPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNV 180
           DPVIAV DGEGGSEHRRLLLPSYKAHRIKFTRQSSSQR+TKGNS R YQVIRDALR+CNV
Sbjct: 121 DPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSCNV 180

Query: 181 PVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240
           PV+K+ GHEADDVVATLV+QVLQRGFR VIASPDKDFKQLISEDVQLVMPLPELNRWSFY
Sbjct: 181 PVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240

Query: 241 TLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300
           TL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS
Sbjct: 241 TLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300

Query: 301 AAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKF 360
           AAA+RTVG+PYAQDALTKYADYLR NYKVLALRRD+DVQF+EEWLV+RDRQNDS ILSKF
Sbjct: 301 AAAVRTVGKPYAQDALTKYADYLRTNYKVLALRRDIDVQFREEWLVDRDRQNDSTILSKF 360

Query: 361 VENNDRNSLVQ 368
           VENNDRNSL +
Sbjct: 361 VENNDRNSLAE 371

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BLAST of MS003542 vs. NCBI nr
Match: XP_022961199.1 (uncharacterized protein LOC111461781 [Cucurbita moschata])

HSP 1 Score: 643.3 bits (1658), Expect = 1.3e-180
Identity = 318/375 (84.80%), Postives = 340/375 (90.67%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLK-PESTLTTKSNSWRTKALQLSAFPVASHST 60
           MAEA ANIG+NIPPFLNS S +SLPSRTLK  ES  TTK+ SWRTK L+LS F  +S ST
Sbjct: 1   MAEASANIGLNIPPFLNSTSPTSLPSRTLKAAESVRTTKAKSWRTKPLKLSVFAASSRST 60

Query: 61  ---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHS 120
              FKQ + G L P +EADNPR GRVFFLDVNPLCY+GSRPSLHNFGRW SIFFE+VS S
Sbjct: 61  SSGFKQAEDGKLPPTMEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVSRS 120

Query: 121 DPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNV 180
           DPVIAV DGEGGSEHRRLLLPSYKAHRIKFTRQSSSQR+TKGNS R YQVIRDALR+CNV
Sbjct: 121 DPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSCNV 180

Query: 181 PVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240
           PV+K+ GHEADDVVATLV+QVLQRGFR VIASPDKDFKQLISEDVQLVMPLPELNRWSFY
Sbjct: 181 PVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240

Query: 241 TLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300
           TL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS
Sbjct: 241 TLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300

Query: 301 AAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKF 360
           AAA+RTVG+PYAQDALTKYADYLR NYKVLALRRD+DVQF+EEWLV+RDRQNDS ILSKF
Sbjct: 301 AAAVRTVGKPYAQDALTKYADYLRTNYKVLALRRDIDVQFREEWLVDRDRQNDSTILSKF 360

Query: 361 VENNDRNSLVQPSKR 372
           VENNDRNSL +   +
Sbjct: 361 VENNDRNSLAEQGNK 375

[Close]

BLAST of MS003542 vs. ExPASy Swiss-Prot
Match: O67550 (5'-3' exonuclease OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1628 PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.6e-24
Identity = 75/258 (29.07%), Postives = 131/258 (50.78%), Query Frame = 0


Query: 84  FLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDP--VIAVFDGEGGSEHRRLLLPSYKA 143
           F  + PL      P+   +G +  + F  +    P  ++ VFD    ++ R  +   YK 
Sbjct: 17  FFALPPLSTSKGFPTNAIYG-FLRMLFSLIKKERPQYLVVVFDAPAKTK-REKIYADYKK 76

Query: 144 HRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVKVDGHEADDVVATLVDQVLQRG 203
            R K       Q            VI++ L+   +P++++ G+EADDV+A L ++  Q+G
Sbjct: 77  QRPKAPDPLKVQ----------IPVIKEILKLAGIPLLELPGYEADDVIAYLAEKFSQKG 136

Query: 204 FRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLRCIMGD 263
           F+V I SPDKD  QL+SE+V ++ P+ +      +T    + ++  +P        ++GD
Sbjct: 137 FKVKIYSPDKDLLQLVSENVLVINPMND----EVFTKERVIKKFGVEPQKIPDYLALVGD 196

Query: 264 EVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRPYAQDALTKYADYLRM 323
           +VD VPGI+    G G KTA+ +LKK+GS+EN+L           + ++      + L +
Sbjct: 197 KVDNVPGIE----GVGPKTAINILKKYGSVENILKN------WEKFQREFPRAKKEDLEL 248

Query: 324 NYKVLALRRDVDVQFQEE 340
           +YK++ L  D+D++  EE
Sbjct: 257 SYKLVKLYTDLDIELSEE 248

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BLAST of MS003542 vs. ExPASy Swiss-Prot
Match: Q04957 (DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 62/207 (29.95%), Postives = 110/207 (53.14%), Query Frame = 0


Query: 135 LLPSYKAHRIKFTRQSSSQRYTKGNSRRP------YQVIRDALRNCNVPVVKVDGHEADD 194
           +L ++ A +  F R  + Q Y  G  + P      + ++R+ LR   +P  +++ +EADD
Sbjct: 54  MLVAFDAGKTTF-RHEAFQEYKGGRQQTPPELSEQFPLLRELLRAYRIPAYELENYEADD 113

Query: 195 VVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCD 254
           ++ TL  +  Q GF V + S D+D  QL S  V + +    +     YT      +Y   
Sbjct: 114 IIGTLAARAEQEGFEVKVISGDRDLTQLASPHVTVDITKKGITDIEPYTPEAVREKYGLT 173

Query: 255 PCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRPYA 314
           P   + L+ +MGD+ D +PG+    PG G KTA+KLL++ G++EN+L  A+I  +     
Sbjct: 174 PEQIVDLKGLMGDKSDNIPGV----PGIGEKTAVKLLRQFGTVENVL--ASIDEIKGEKL 233

Query: 315 QDALTKYADYLRMNYKVLALRRDVDVQ 336
           ++ L ++ +   ++ K+ A+RRD  V+
Sbjct: 234 KETLRQHREMALLSKKLAAIRRDAPVE 253

[Close]

BLAST of MS003542 vs. ExPASy Swiss-Prot
Match: P52026 (DNA polymerase I OS=Geobacillus stearothermophilus OX=1422 GN=polA PE=1 SV=2)

HSP 1 Score: 100.9 bits (250), Expect = 3.2e-20
Identity = 62/224 (27.68%), Postives = 116/224 (51.79%), Query Frame = 0


Query: 135 LLPSYKAHRIKFTRQSSSQRYTKGNSRRP------YQVIRDALRNCNVPVVKVDGHEADD 194
           +L ++ A +  F R  + Q Y  G  + P      + ++R+ L+   +P  ++D +EADD
Sbjct: 54  ILVAFDAGKTTF-RHETFQDYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADD 113

Query: 195 VVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCD 254
           ++ T+  +  + GF V + S D+D  QL S  V + +    +     YT    + +Y   
Sbjct: 114 IIGTMAARAEREGFAVKVISGDRDLTQLASPQVTVEITKKGITDIESYTPETVVEKYGLT 173

Query: 255 PCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRPYA 314
           P   + L+ +MGD+ D +PG+    PG G KTA+KLLK+ G++EN+L  A+I  +     
Sbjct: 174 PEQIVDLKGLMGDKSDNIPGV----PGIGEKTAVKLLKQFGTVENVL--ASIDEIKGEKL 233

Query: 315 QDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAI 353
           ++ L +Y D   ++ ++ A+ RD  V+   + +V +    +  +
Sbjct: 234 KENLRQYRDLALLSKQLAAICRDAPVELTLDDIVYKGEDREKVV 270

[Close]

BLAST of MS003542 vs. ExPASy Swiss-Prot
Match: Q9RLB6 (DNA polymerase I OS=Rickettsia helvetica OX=35789 GN=polA PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 6.0e-19
Identity = 63/219 (28.77%), Postives = 107/219 (48.86%), Query Frame = 0


Query: 119 VIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPV 178
           V  VFD  GG   R  + P YKA+R         Q            ++RD   N N P+
Sbjct: 56  VAVVFD-SGGKNFRHYIYPEYKANRPPPPEDLIVQ----------LPLVRDVASNLNFPI 115

Query: 179 VKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTL 238
           ++ +G+EADD++AT   +    G  VVI S DKD  QL+SE++++  PL    R  + T 
Sbjct: 116 LEKNGYEADDIIATFATKTAALGENVVIISSDKDLLQLMSENIKIYDPL----RGKYITE 175

Query: 239 RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAA 298
              + ++         +  ++GD  D +PG+    P  G KTA  L+ + GS+EN+ +  
Sbjct: 176 DDVVKKFGTTSDKLREVMALIGDRSDNIPGV----PSIGPKTASSLITQFGSVENIFN-- 235

Query: 299 AIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQ 338
           ++  V     ++ L    +   ++++++ L  +VD+ FQ
Sbjct: 236 SLDQVSSVKQRETLQNSREAALISWQLIGLDSNVDLDFQ 253

[Close]

BLAST of MS003542 vs. ExPASy Swiss-Prot
Match: Q92GB7 (DNA polymerase I OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=polA PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 61/219 (27.85%), Postives = 107/219 (48.86%), Query Frame = 0


Query: 119 VIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPV 178
           V  VFD  GG   R  + P YKA+R         Q            ++RD   N N P+
Sbjct: 56  VAVVFD-SGGKNFRHQIYPDYKANRPPPPEDLIIQ----------LPLVRDVASNLNFPI 115

Query: 179 VKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTL 238
           ++ +G+EADD++AT   +    G  VVI S DKD  QL++E++++  PL    +  + T 
Sbjct: 116 LEKNGYEADDIIATFATKTAALGAHVVIISSDKDLLQLMTENIKIYDPL----KGKYITE 175

Query: 239 RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAA 298
              + ++         +  ++GD  D +PG+    P  G KTA  L+ + GS+EN+ +  
Sbjct: 176 DDVVKKFGTTSDKLREVMALIGDRSDNIPGV----PSIGPKTASSLITQFGSVENIFN-- 235

Query: 299 AIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQ 338
           ++  V     ++ L    +   ++++++ L  +VD+ FQ
Sbjct: 236 SLDQVSSVKQRETLQNSREAALISWQLIGLNSNVDLDFQ 253

[Close]

BLAST of MS003542 vs. ExPASy TrEMBL
Match: A0A6J1DGI1 (uncharacterized protein LOC111020233 OS=Momordica charantia OX=3673 GN=LOC111020233 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.6e-211
Identity = 370/372 (99.46%), Postives = 370/372 (99.46%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF 60
           MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF
Sbjct: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHSTF 60

Query: 61  KQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI 120
           KQTDGGTLQPIIEADNPR GRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI
Sbjct: 61  KQTDGGTLQPIIEADNPRKGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVI 120

Query: 121 AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK 180
           AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK
Sbjct: 121 AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK 180

Query: 181 VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH 240
           VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH
Sbjct: 181 VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH 240

Query: 241 YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI 300
           YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI
Sbjct: 241 YLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAI 300

Query: 301 RTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN 360
           RTVGRPYAQDALTKYADYLR NYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN
Sbjct: 301 RTVGRPYAQDALTKYADYLRTNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFVENN 360

Query: 361 DRNSLVQPSKRV 373
           DRNSLVQPSKRV
Sbjct: 361 DRNSLVQPSKRV 372

[Close]

BLAST of MS003542 vs. ExPASy TrEMBL
Match: A0A6J1JHT6 (uncharacterized protein LOC111487120 OS=Cucurbita maxima OX=3661 GN=LOC111487120 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 323/371 (87.06%), Postives = 341/371 (91.91%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLK-PESTLTTKSNSWRTKALQLSAFPVASHST 60
           MAEA ANIG+NIPPFLNSAS +SLPSRTLK  ES  TTK+ SWRTK L+LS F  AS ST
Sbjct: 1   MAEASANIGLNIPPFLNSASPTSLPSRTLKAAESVRTTKAKSWRTKPLKLSVFAAASRST 60

Query: 61  ---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHS 120
              FKQ + G L P +EADNPR GRVFFLDVNPLCY+GSRPSLHNFGRW SIFFE+VSHS
Sbjct: 61  SSGFKQAEDGKLPPRMEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVSHS 120

Query: 121 DPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNV 180
           DPVIAV DGEGGSEHRRLLLPSYKAHRIKFTRQSSSQR+TKGNS R YQVIRDALR+C+V
Sbjct: 121 DPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSCDV 180

Query: 181 PVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240
           PV+K+ GHEADDVVATLV+QVLQRGFR VIASPDKDFKQLISEDVQLVMPLPELNRWSFY
Sbjct: 181 PVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240

Query: 241 TLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300
           TL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS
Sbjct: 241 TLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300

Query: 301 AAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKF 360
           AAAIRTVG+PYAQDALTKYADYLR NYKVLALRRDVDVQF+EEWLVERDRQNDS ILSKF
Sbjct: 301 AAAIRTVGKPYAQDALTKYADYLRTNYKVLALRRDVDVQFREEWLVERDRQNDSTILSKF 360

Query: 361 VENNDRNSLVQ 368
           VENNDRNSL +
Sbjct: 361 VENNDRNSLAE 371

[Close]

BLAST of MS003542 vs. ExPASy TrEMBL
Match: A0A6J1HDC1 (uncharacterized protein LOC111461781 OS=Cucurbita moschata OX=3662 GN=LOC111461781 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.4e-181
Identity = 318/375 (84.80%), Postives = 340/375 (90.67%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLK-PESTLTTKSNSWRTKALQLSAFPVASHST 60
           MAEA ANIG+NIPPFLNS S +SLPSRTLK  ES  TTK+ SWRTK L+LS F  +S ST
Sbjct: 1   MAEASANIGLNIPPFLNSTSPTSLPSRTLKAAESVRTTKAKSWRTKPLKLSVFAASSRST 60

Query: 61  ---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHS 120
              FKQ + G L P +EADNPR GRVFFLDVNPLCY+GSRPSLHNFGRW SIFFE+VS S
Sbjct: 61  SSGFKQAEDGKLPPTMEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVSRS 120

Query: 121 DPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNV 180
           DPVIAV DGEGGSEHRRLLLPSYKAHRIKFTRQSSSQR+TKGNS R YQVIRDALR+CNV
Sbjct: 121 DPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSCNV 180

Query: 181 PVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240
           PV+K+ GHEADDVVATLV+QVLQRGFR VIASPDKDFKQLISEDVQLVMPLPELNRWSFY
Sbjct: 181 PVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWSFY 240

Query: 241 TLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300
           TL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS
Sbjct: 241 TLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLS 300

Query: 301 AAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKF 360
           AAA+RTVG+PYAQDALTKYADYLR NYKVLALRRD+DVQF+EEWLV+RDRQNDS ILSKF
Sbjct: 301 AAAVRTVGKPYAQDALTKYADYLRTNYKVLALRRDIDVQFREEWLVDRDRQNDSTILSKF 360

Query: 361 VENNDRNSLVQPSKR 372
           VENNDRNSL +   +
Sbjct: 361 VENNDRNSLAEQGNK 375

[Close]

BLAST of MS003542 vs. ExPASy TrEMBL
Match: A0A0A0LQN4 (53EXOc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402050 PE=4 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.5e-179
Identity = 317/377 (84.08%), Postives = 341/377 (90.45%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTT--KSNSWRTKALQLSAFPVASHS 60
           MAEA ANIGVN PPFLNS+S + LPSRTLKPE  LT+  K N+WRTK L L+AF  +S  
Sbjct: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPEFPLTSKLKPNTWRTKPLNLTAFAPSSRL 60

Query: 61  T---FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSH 120
           T   F QTD G  QP IEADN R GRVFFLDVNPLCY+GS+PSL NFGRW SIFFE+VSH
Sbjct: 61  TSAAFTQTDDGKFQPRIEADNSRTGRVFFLDVNPLCYQGSKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTR  SS+R+TKGN R  YQVIRDALR+CN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLV+QVLQRG RVV+ASPDKDFKQLISED+QLVMPLPELNRWSF
Sbjct: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDIQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLL 300
           YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENLL
Sbjct: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSK 360
           SAAAIRTVG+PYAQDALTKYA+YLR NYKVLALRRDVDVQFQ+EWLVERDR+NDS ILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNSLVQPSKRV 373
           FVENNDRN LVQPSK+V
Sbjct: 361 FVENNDRNLLVQPSKQV 377

[Close]

BLAST of MS003542 vs. ExPASy TrEMBL
Match: A0A1S3B2X7 (5'-3' exonuclease OS=Cucumis melo OX=3656 GN=LOC103485414 PE=4 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.3e-177
Identity = 315/375 (84.00%), Postives = 336/375 (89.60%), Query Frame = 0


Query: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTTKSNSWRTKALQLSAFPVASHST- 60
           M EA A IGVN PPFLNS+SR+ LPSRT     T   K N+WRTK L+L+AF  +S  T 
Sbjct: 1   MVEASAKIGVNFPPFLNSSSRTFLPSRT---ALTSKLKPNTWRTKPLKLTAFVPSSRLTS 60

Query: 61  --FKQTDGGTLQPIIEADNPRNGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSHSD 120
             F QTD G  QP IEADNPR GRVFFLDVNPLCY+G++PSL NFGRW SIFFE+VSHSD
Sbjct: 61  AAFNQTDDGKFQPRIEADNPRKGRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSD 120

Query: 121 PVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVP 180
           PVIAVFDGEGGSEHRRLLLPSYKAHRIKFTR  SSQR+TKGN R  YQVIRDALR+CNVP
Sbjct: 121 PVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSQRFTKGNFRTSYQVIRDALRSCNVP 180

Query: 181 VVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYT 240
           VVKVDGHEADDVVATLV+QVLQRG RVV+ASPDKDFKQLISEDVQLVMPLPELNRWSFYT
Sbjct: 181 VVKVDGHEADDVVATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYT 240

Query: 241 LRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSA 300
           +RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENLLSA
Sbjct: 241 IRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSA 300

Query: 301 AAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSKFV 360
           AAIRTVG+PYAQDALTKYA+YLR NYKVLALRRDVDVQFQ+EWLVERDR+NDS ILSKFV
Sbjct: 301 AAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSKFV 360

Query: 361 ENNDRNSLVQPSKRV 373
           ENNDRN LVQPSK+V
Sbjct: 361 ENNDRNLLVQPSKQV 372

[Close]

BLAST of MS003542 vs. TAIR 10
Match: AT1G34380.2 (5'-3' exonuclease family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 3.2e-116
Identity = 212/331 (64.05%), Postives = 256/331 (77.34%), Query Frame = 0


Query: 38  KSNSWRTKALQLSAFPVASHS---TFKQTDGGTLQPIIEAD---------NPRNGRVFFL 97
           K+   RTK +  S+   +SHS   TF +T  G +Q +++ D           +N RVFFL
Sbjct: 22  KTQPSRTKWVSSSSSSFSSHSSVETFHRT--GNVQ-VLQKDVLCGNNEEIRKKNKRVFFL 81

Query: 98  DVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVIAVFDGEGGSEHRRLLLPSYKAHRIK 157
           DV+PLCYEG++PS   FG W S+FF QVS +DPVIAV DGE G++ RR LLPSYKAHR  
Sbjct: 82  DVSPLCYEGNKPSSQAFGHWISLFFSQVSLTDPVIAVIDGEEGNQRRRELLPSYKAHR-- 141

Query: 158 FTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVKVDGHEADDVVATLVDQVLQRGFRVV 217
             +  +  RY    S+RP+Q + + LR CNVPVV+++GHEADDVVATL++Q +QRG+R V
Sbjct: 142 --KSPNHGRY----SKRPHQFVDEVLRKCNVPVVRIEGHEADDVVATLMEQAVQRGYRAV 201

Query: 218 IASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLRCIMGDEVDG 277
           IASPDKDFKQLISE+VQ+V+PL +L RWSFYTL+HY AQYNCDP SDLS RCIMGDEVDG
Sbjct: 202 IASPDKDFKQLISENVQIVIPLADLRRWSFYTLKHYHAQYNCDPQSDLSFRCIMGDEVDG 261

Query: 278 VPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRPYAQDALTKYADYLRMNYKV 337
           VPGIQH+ P FGRKTAMKL++KHGSLE+LLSAAA+RTVGRPYAQ+ALTKYADYLR NY+V
Sbjct: 262 VPGIQHMVPAFGRKTAMKLVRKHGSLESLLSAAAVRTVGRPYAQEALTKYADYLRRNYQV 321

Query: 338 LALRRDVDVQFQEEWLVERDRQNDSAILSKF 357
           LAL RDV VQ Q EWL+ERD  NDS +LS F
Sbjct: 322 LALNRDVRVQIQNEWLMERDTSNDSEVLSSF 341

[Close]

BLAST of MS003542 vs. TAIR 10
Match: AT1G34380.1 (5'-3' exonuclease family protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.4e-66
Identity = 132/231 (57.14%), Postives = 167/231 (72.29%), Query Frame = 0


Query: 38  KSNSWRTKALQLSAFPVASHS---TFKQTDGGTLQPIIEAD---------NPRNGRVFFL 97
           K+   RTK +  S+   +SHS   TF +T  G +Q +++ D           +N RVFFL
Sbjct: 22  KTQPSRTKWVSSSSSSFSSHSSVETFHRT--GNVQ-VLQKDVLCGNNEEIRKKNKRVFFL 81

Query: 98  DVNPLCYEGSRPSLHNFGRWASIFFEQVSHSDPVIAVFDGEGGSEHRRLLLPSYKAHRIK 157
           DV+PLCYEG++PS   FG W S+FF QVS +DPVIAV DGE G++ RR LLPSYKAHR  
Sbjct: 82  DVSPLCYEGNKPSSQAFGHWISLFFSQVSLTDPVIAVIDGEEGNQRRRELLPSYKAHR-- 141

Query: 158 FTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVKVDGHEADDVVATLVDQVLQRGFRVV 217
             +  +  RY    S+RP+Q + + LR CNVPVV+++GHEADDVVATL++Q +QRG+R V
Sbjct: 142 --KSPNHGRY----SKRPHQFVDEVLRKCNVPVVRIEGHEADDVVATLMEQAVQRGYRAV 201

Query: 218 IASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLR 257
           IASPDKDFKQLISE+VQ+V+PL +L RWSFYTL+HY AQYNCDP SDLS R
Sbjct: 202 IASPDKDFKQLISENVQIVIPLADLRRWSFYTLKHYHAQYNCDPQSDLSFR 241

[Close]

BLAST of MS003542 vs. TAIR 10
Match: AT3G52050.4 (5'-3' exonuclease family protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.9e-20
Identity = 61/220 (27.73%), Postives = 114/220 (51.82%), Query Frame = 0


Query: 119 VIAVFDGEG-----GSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRN 178
           V  VFD +G     G   R  L P+YK++R            T     +  Q ++ +++ 
Sbjct: 152 VAVVFDHDGYRSAKGMNFRHTLYPAYKSNRPP----------TPDTIVQGLQYLKASIKA 211

Query: 179 CNVPVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRW 238
            ++ V++V G EADDV+ TL  + +  GF+V + SPDKDF Q++S  ++L+   P  +  
Sbjct: 212 MSIKVIEVPGVEADDVIGTLAMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEM 271

Query: 239 SFYTLRHYLAQY-NCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLE 298
           + + +  +  ++ N +P   + +  + GD+ D +PG+     G G   A++L+ + G+LE
Sbjct: 272 ASFGMEDFAKKFGNLEPAQFVDIIALAGDKSDNIPGVD----GIGNVHAVELISRFGTLE 331

Query: 299 NLLSAAAIRTVGRPYAQDALTKYADYLRMNYKVLALRRDV 333
           NLL   ++  +     +++L   AD   ++ K+  LR D+
Sbjct: 332 NLLQ--SVDEIKEGKIKESLIASADQAILSKKLALLRSDL 355

[Close]

BLAST of MS003542 vs. TAIR 10
Match: AT3G52050.5 (5'-3' exonuclease family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-19
Identity = 64/238 (26.89%), Postives = 111/238 (46.64%), Query Frame = 0


Query: 79  NGRVFFLDVNPLCYEGSRPSL---------HNFGR--WASIFFEQVSHSDPV-------I 138
           NGRV  +D   + Y      L         H  G   W    F  +S    V       +
Sbjct: 116 NGRVMLIDGTSIIYRAYYKLLARLNHGHLAHADGNADWVLTIFSSLSLLIDVLKFLPSHV 175

Query: 139 AVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVK 198
           AV     G   R  L P+YK++R            T     +  Q ++ +++  ++ V++
Sbjct: 176 AVVFDHDGMNFRHTLYPAYKSNRPP----------TPDTIVQGLQYLKASIKAMSIKVIE 235

Query: 199 VDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRH 258
           V G EADDV+ TL  + +  GF+V + SPDKDF Q++S  ++L+   P  +  + + +  
Sbjct: 236 VPGVEADDVIGTLAMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMED 295

Query: 259 YLAQY-NCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSA 298
           +  ++ N +P   + +  + GD+ D +PG+     G G   A++L+ + G+LENLL +
Sbjct: 296 FAKKFGNLEPAQFVDIIALAGDKSDNIPGVD----GIGNVHAVELISRFGTLENLLQS 339

[Close]

BLAST of MS003542 vs. TAIR 10
Match: AT3G52050.2 (5'-3' exonuclease family protein )

HSP 1 Score: 93.6 bits (231), Expect = 3.6e-19
Identity = 56/206 (27.18%), Postives = 108/206 (52.43%), Query Frame = 0


Query: 128 GSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCNVPVVKVDGHEAD 187
           G   R  L P+YK++R            T     +  Q ++ +++  ++ V++V G EAD
Sbjct: 178 GMNFRHTLYPAYKSNRPP----------TPDTIVQGLQYLKASIKAMSIKVIEVPGVEAD 237

Query: 188 DVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQY-N 247
           DV+ TL  + +  GF+V + SPDKDF Q++S  ++L+   P  +  + + +  +  ++ N
Sbjct: 238 DVIGTLAMRSISAGFKVRVVSPDKDFFQILSPSLRLLRLTPRGSEMASFGMEDFAKKFGN 297

Query: 248 CDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLLSAAAIRTVGRP 307
            +P   + +  + GD+ D +PG+     G G   A++L+ + G+LENLL   ++  +   
Sbjct: 298 LEPAQFVDIIALAGDKSDNIPGVD----GIGNVHAVELISRFGTLENLLQ--SVDEIKEG 357

Query: 308 YAQDALTKYADYLRMNYKVLALRRDV 333
             +++L   AD   ++ K+  LR D+
Sbjct: 358 KIKESLIASADQAILSKKLALLRSDL 367

The following BLAST results are available for this feature:

BLAST of MS003542 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152532.1	3.2e-211	99.46	uncharacterized protein LOC111020233 [Momordica charantia]	[more]
XP_038884032.1	2.4e-182	86.24	5'-3' exonuclease [Benincasa hispida]	[more]
XP_022990137.1	5.3e-182	87.06	uncharacterized protein LOC111487120 [Cucurbita maxima]	[more]
KAG7033404.1	4.5e-181	85.71	hypothetical protein SDJN02_07460 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961199.1	1.3e-180	84.80	uncharacterized protein LOC111461781 [Cucurbita moschata]	[more]

BLAST of MS003542 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67550	3.6e-24	29.07	5'-3' exonuclease OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1628 PE=3 SV=...	[more]
Q04957	1.1e-20	29.95	DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1	[more]
P52026	3.2e-20	27.68	DNA polymerase I OS=Geobacillus stearothermophilus OX=1422 GN=polA PE=1 SV=2	[more]
Q9RLB6	6.0e-19	28.77	DNA polymerase I OS=Rickettsia helvetica OX=35789 GN=polA PE=3 SV=1	[more]
Q92GB7	1.7e-18	27.85	DNA polymerase I OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944...	[more]

BLAST of MS003542 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGI1	1.6e-211	99.46	uncharacterized protein LOC111020233 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1JHT6	2.6e-182	87.06	uncharacterized protein LOC111487120 OS=Cucurbita maxima OX=3661 GN=LOC111487120...	[more]
A0A6J1HDC1	6.4e-181	84.80	uncharacterized protein LOC111461781 OS=Cucurbita moschata OX=3662 GN=LOC1114617...	[more]
A0A0A0LQN4	3.5e-179	84.08	53EXOc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402050 PE=4...	[more]
A0A1S3B2X7	3.3e-177	84.00	5'-3' exonuclease OS=Cucumis melo OX=3656 GN=LOC103485414 PE=4 SV=1	[more]

BLAST of MS003542 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G34380.2	3.2e-116	64.05	5'-3' exonuclease family protein	[more]
AT1G34380.1	1.4e-66	57.14	5'-3' exonuclease family protein	[more]
AT3G52050.4	1.9e-20	27.73	5'-3' exonuclease family protein	[more]
AT3G52050.5	1.2e-19	26.89	5'-3' exonuclease family protein	[more]
AT3G52050.2	3.6e-19	27.18	5'-3' exonuclease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002421	5'-3' exonuclease	SMART	SM00475	53exo3	coord: 80..344 e-value: 1.3E-20 score: 84.5
None	No IPR available	GENE3D	1.10.150.20		coord: 245..326 e-value: 1.8E-18 score: 68.4
None	No IPR available	GENE3D	3.40.50.1010		coord: 72..244 e-value: 1.0E-28 score: 101.7
None	No IPR available	PANTHER	PTHR10133:SF52	5'-3' EXONUCLEASE FAMILY PROTEIN	coord: 1..363
None	No IPR available	CDD	cd09859	PIN_53EXO	coord: 117..226 e-value: 2.16584E-24 score: 95.8952
IPR020046	5'-3' exonuclease, alpha-helical arch, N-terminal	PFAM	PF02739	5_3_exonuc_N	coord: 112..225 e-value: 1.0E-21 score: 77.5
IPR020045	DNA polymerase I-like, H3TH domain	PFAM	PF01367	5_3_exonuc	coord: 255..339 e-value: 1.8E-14 score: 54.1
IPR020045	DNA polymerase I-like, H3TH domain	CDD	cd09898	H3TH_53EXO	coord: 258..328 e-value: 5.81434E-15 score: 67.0389
IPR002298	DNA polymerase A	PANTHER	PTHR10133	DNA POLYMERASE I	coord: 1..363
IPR036279	5'-3' exonuclease, C-terminal domain superfamily	SUPERFAMILY	47807	5' to 3' exonuclease, C-terminal subdomain	coord: 255..355
IPR029060	PIN-like domain superfamily	SUPERFAMILY	88723	PIN domain-like	coord: 81..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003542.1	MS003542.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006261	DNA-dependent DNA replication
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity