MS003532 (gene) Bitter gourd (TR) v1

Overview


Name	MS003532
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	alkylated DNA repair protein alkB homolog 8 isoform X1
Location	scaffold234: 3333614 .. 3335763 (+)
RNA-Seq Expression	MS003532
Synteny	MS003532

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCACCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTAGTCCCCGTATTCGTAGTCCTTCATCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATGGTTCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTAATGATGAAAACTTATTAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCAGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTATTCCTCTCTCTCATCTATTGTATGGTTTTCATTTTCTGGTCTCCTTTTGCTTATTGGGTACGAGTGAGTCACTTGAATAGATCCGGTGCCCTCTTGCATGAGTTCTATAATCTATAATGTAACAAATATCTCTATACAGATGTGACTGTGCTGTTCTAGATATGTTCCATCTCTCATTTCCTCCCTCTAATACTCAAACATGAGCCATATATTTATGTTTAAACATAGCTGGATGAAAGTTGGACAAAAAGGGAATCATGTAGACATTTTAATATATGTGCAAAAAAAATAAATCATCTTCATAATGCACAAAGCAACAGGGTTGAAAGTAGTCTCGGTGGTAGAAAAGGTTGTTTTTGCACATGGGAGATGGCGCCGGTACTGTTGATTACTCCTTTTAGTCCAAGTTGGAAATATATGGAAAAAGAACATTATACTTTTCCTAGCTTTGACTTATTGTTTCCCTACGAGCAAAATCAATCAGAAATTTCTACCGTGAAAAAGAGATTTTTACAAAAGAGGAAAGGGGAGATAATAAGGCATCCATACCCAAGACAAAAAGGATCTTCACCAATTGGTAGCAATCTAAAAGGGGGCTACAGTATTAAGGCCAAAATAAGAAGAAGAAAATAAGGAAAAATTGAACCTAACCTTTTGGCTGAAAGTTGGCCCTACCAAATTTCCTCTGCTATATTAGATGTTTACTTGGGGGCACCTATTGGAAGTTTGCCTTGTATAATTTGCACGTGGAGTAAATGTTAATATATGTATGCAGTAGCTTCCATTAATGTGTGCTCAGGTGATAATCAATTTTCTATCATTCCATAGTTTGACTTGAAATTCCATCAACTTTTGAAATATAGGGAACTCAGTATACCATACTTCAAGACTTCAACTTATTTCACCTTCCTTTACCCCCCTCGAGATACGTTTCTTAGGTAATATTTTGTTCTTTTACTGTTTGGGTGCAGGCTAGTTTCTGGCATGGACAATGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTG

mRNA sequence

ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCACCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTAGTCCCCGTATTCGTAGTCCTTCATCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATGGTTCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTAATGATGAAAACTTATTAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCAGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGCTAGTTTCTGGCATGGACAATGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTG

Coding sequence (CDS)

ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCACCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTAGTCCCCGTATTCGTAGTCCTTCATCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATGGTTCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTAATGATGAAAACTTATTAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCAGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGCTAGTTTCTGGCATGGACAATGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTG

Protein sequence

MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSCNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV

Homology

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BLAST of MS003532 vs. NCBI nr
Match: XP_022152525.1 (alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia] >XP_022152526.1 alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia])

HSP 1 Score: 783.5 bits (2022), Expect = 8.6e-223
Identity = 387/392 (98.72%), Postives = 388/392 (98.98%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE
Sbjct: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60

Query: 61  TNAQQSPCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120
           TNAQQS CANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG
Sbjct: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120

Query: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180
           SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240

Query: 241 WVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300
           WVGPGSPR+RSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC
Sbjct: 241 WVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300

Query: 301 NDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
            DENL KTAQQEYFVPWHLPYHRAEVS TSASALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 392

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BLAST of MS003532 vs. NCBI nr
Match: XP_022152527.1 (alkylated DNA repair protein alkB homolog 8 isoform X2 [Momordica charantia])

HSP 1 Score: 704.9 bits (1818), Expect = 3.9e-199
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0


Query: 41  MKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 100
           MKEIKVKAGSKSDPHLSNDETNAQQS CANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS
Sbjct: 1   MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 60

Query: 101 STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 160
           STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH
Sbjct: 61  STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 120

Query: 161 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 220
           DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE
Sbjct: 121 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 180

Query: 221 QEDKSLLTKWMPLTEKYVEEWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFT 280
           QEDKSLLTKWMPLTEKYVEEWVGPGSPR+RSPSSLALESIPEMNENGSGIHLRDSKENFT
Sbjct: 181 QEDKSLLTKWMPLTEKYVEEWVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFT 240

Query: 281 GSMPENKSLPSQSGDELPSCNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAK 340
           GSMPENKSLPSQSGDELPSC DENL KTAQQEYFVPWHLPYHRAEVS TSASALASGLAK
Sbjct: 241 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 300

Query: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 352

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BLAST of MS003532 vs. NCBI nr
Match: XP_023518340.1 (alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.6 bits (1781), Expect = 7.6e-195
Identity = 343/393 (87.28%), Postives = 364/393 (92.62%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQASYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CA QGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLPL
Sbjct: 61  AHAPQLSCATQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPL 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSL+LDAGCGNGKYLGF PNCFFIGCDISAQLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRR+KAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYV+
Sbjct: 181 AISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN +G+ L+DSKENFTGS PENK LPS+SG++  +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENHAGVSLKDSKENFTGSKPENKPLPSRSGNDFMN 300

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENLLK  QQEYFVPWHLPYHRAEVS +SASALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLLK-IQQEYFVPWHLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMD+AVV+DRFYDKSNWC+VLEKTV
Sbjct: 361 GELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 392

[Close]

BLAST of MS003532 vs. NCBI nr
Match: XP_022939521.1 (alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-193
Identity = 345/393 (87.79%), Postives = 360/393 (91.60%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN SG +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENL KT QQEYFVPWHLPYHRAEVS TSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 354 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 413

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 414 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of MS003532 vs. NCBI nr
Match: XP_022939523.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-193
Identity = 345/393 (87.79%), Postives = 360/393 (91.60%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN SG +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENL KT QQEYFVPWHLPYHRAEVS TSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

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BLAST of MS003532 vs. ExPASy Swiss-Prot
Match: Q94A09 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=TRM9 PE=1 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 5.5e-132
Identity = 248/380 (65.26%), Postives = 282/380 (74.21%), Query Frame = 0


Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG----SPRIRSPSSLALESIPEMN-- 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG    SPR+R+    +LESIPE    
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 ------ENGSGIHLRDSKENFTGS--------MPENKSLPSQSGDELPSCNDENLLKTAQ 330
                 EN   I L    E+   +        +PE K+   +  DE         LK +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 390
           QEYFVPWHLPYHRAEVS  SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

[Close]

BLAST of MS003532 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 5.0e-56
Identity = 119/309 (38.51%), Postives = 169/309 (54.69%), Query Frame = 0


Query: 83  IEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFF 142
           +E++YVH+VYD IA HFSSTR   WPK+  FL+SLP GSL+ D GCGNGKYLG   +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 143 IGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 202
           IGCD S  L+ IC+ER  +  V DA+++P+R G  DA ISIAV+HH +TE RR  A++EL
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQEL 488

Query: 203 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPGSPRIRSPSSLALESIPE 262
           IR+++KGG  LI VWA+EQE K   +K++                               
Sbjct: 489 IRLLRKGGKALIYVWALEQEYKKNKSKYL------------------------------- 548

Query: 263 MNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSCNDENLLKTAQQEYFVPWHL-PY 322
                        KE+ T   P + +L +    + P     N      Q+  VPWHL P 
Sbjct: 549 -------------KESKTSQGPSSDALGTNLAVKAPLPIHTNRTSFHSQDLLVPWHLKPT 608

Query: 323 HRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDK 382
           ++++V+  +          + +     VY+R+YHVF EGELE + + + N  V   ++D+
Sbjct: 609 NKSKVTPENKE------QNEKEHGPDSVYHRFYHVFCEGELEAMCNRLSNVAVQHSYHDQ 627

Query: 383 SNWCIVLEK 391
            NWC++LEK
Sbjct: 669 GNWCVILEK 627

[Close]

BLAST of MS003532 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.6e-54
Identity = 116/313 (37.06%), Postives = 170/313 (54.31%), Query Frame = 0


Query: 82  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCF 141
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS++ D GCGNGKYLG   + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 142 FIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 201
            IGCD S  L+ IC+ER    LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 202 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPGSPRIRSPSSLALESIP 261
           L R+++ GG  LI VWA+EQE K+  +K+  L  K + +          S     +   P
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKY--LRGKRISQGDKDELNSATSTEEFLVNQTP 548

Query: 262 EMNENGSGIHLRDS----KENFTGSMPENKSLPSQSGDELPSCNDENLLKTAQQEYFVPW 321
           E       + +  S    +E +      N  LP             N      Q+  VPW
Sbjct: 549 EGVNEDPALSVNSSSITKEEEYKSRKVPNSELPIHI----------NRTCFHSQDVLVPW 608

Query: 322 HLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDR 381
           HL  +  +  +   S +A           + V++RYYHVF +GELE     + +  ++  
Sbjct: 609 HLKRNPGKDKAIEPSGVAG------CPDPSPVFHRYYHVFCDGELEASCQAVGDVSILQS 663

Query: 382 FYDKSNWCIVLEK 391
           +YD+ NWC+VL+K
Sbjct: 669 YYDQGNWCVVLQK 663

[Close]

BLAST of MS003532 vs. ExPASy Swiss-Prot
Match: A1A4L5 (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.6e-54
Identity = 122/341 (35.78%), Postives = 177/341 (51.91%), Query Frame = 0


Query: 64  QQSPCANQ---GCSSNVQCTP-----------EIEKKYVHRVYDAIAPHFSSTRFAKWPK 123
           +Q+PC       C S ++ TP           ++E+ YVHRVY+ IA HFSSTR   WP 
Sbjct: 337 RQTPCNCSYPLVCDSQMKETPASFPESDKEALQLEQDYVHRVYEEIARHFSSTRHTPWPH 396

Query: 124 VAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVN 183
           +  FL +LP GSL+ D GCGNGKYLG     + IGCD S  L+ IC+ER +   V DA+ 
Sbjct: 397 IVEFLKALPSGSLVADIGCGNGKYLGINNELYMIGCDHSQNLVDICRERQYQAFVCDALA 456

Query: 184 LPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTK 243
           +P R+G  DA ISIAV+HH +T  RR  A++EL+R+++ GG  LI VWA+EQE     +K
Sbjct: 457 VPIRSGSCDACISIAVIHHFATAERRVAALQELVRLLRPGGKALIYVWAMEQEYNKKKSK 516

Query: 244 WMPLTEKYVEEWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSL 303
           +  L E    + +        S   L ++ +P +  N    H   S  +F      +K++
Sbjct: 517 Y--LRENRTSQGMKEEISNDASVQELLVKQLPNVG-NQDSAHSVSSINDFQDGGCNSKNV 576

Query: 304 PSQSGDELPSCNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVV 363
              +  +LP     N      Q+  VPWH         +               +    V
Sbjct: 577 ---ANSKLPI--HTNRTSFHSQDLLVPWHFK------GNPGKDKRVEPFGPLGSRDPGPV 636

Query: 364 YNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEK 391
           ++RYYHVF EGELE     ++N  ++  +YD+ NWC++L+K
Sbjct: 637 FHRYYHVFCEGELEAACRTLNNISILQSYYDQGNWCVILQK 663

[Close]

BLAST of MS003532 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.7e-52
Identity = 114/314 (36.31%), Postives = 167/314 (53.18%), Query Frame = 0


Query: 83  IEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFF 142
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS++ D GCGNGKYLG     + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 143 IGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 202
           +GCD S  L+ IC+ER     V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 203 IRVVKKGGLVLITVWAVEQEDKSLLTKWMP--LTEKYVEEWVGPGSPRIRSPSSLALESI 262
           +R+++ GG  LI VWA+EQE     +K++      +  +E +   +   RS     +E +
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGKKEEMNSDTSVQRS----LVEQM 549

Query: 263 PEMNENGSGI---HLRDSKENFTGS-MPENKSLPSQSGDELPSCNDENLLKTAQQEYFVP 322
           P+M    S      + DS+E    S    N  LP             N      Q+  VP
Sbjct: 550 PDMGSRDSASSVPRINDSQEGGCNSRQVSNSKLPIH----------VNRTSFYSQDMLVP 609

Query: 323 WHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVID 382
           WHL        +               +  + V++RYYHVF EGELE L   + +  ++ 
Sbjct: 610 WHLK------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSDVRILQ 663

Query: 383 RFYDKSNWCIVLEK 391
            +YD+ NWC++L+K
Sbjct: 670 SYYDQGNWCVILQK 663

[Close]

BLAST of MS003532 vs. ExPASy TrEMBL
Match: A0A6J1DE66 (alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020230 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.2e-223
Identity = 387/392 (98.72%), Postives = 388/392 (98.98%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE
Sbjct: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60

Query: 61  TNAQQSPCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120
           TNAQQS CANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG
Sbjct: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120

Query: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180
           SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240

Query: 241 WVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300
           WVGPGSPR+RSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC
Sbjct: 241 WVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300

Query: 301 NDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
            DENL KTAQQEYFVPWHLPYHRAEVS TSASALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 392

[Close]

BLAST of MS003532 vs. ExPASy TrEMBL
Match: A0A6J1DGH6 (alkylated DNA repair protein alkB homolog 8 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020230 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.9e-199
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0


Query: 41  MKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 100
           MKEIKVKAGSKSDPHLSNDETNAQQS CANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS
Sbjct: 1   MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 60

Query: 101 STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 160
           STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH
Sbjct: 61  STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 120

Query: 161 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 220
           DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE
Sbjct: 121 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 180

Query: 221 QEDKSLLTKWMPLTEKYVEEWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFT 280
           QEDKSLLTKWMPLTEKYVEEWVGPGSPR+RSPSSLALESIPEMNENGSGIHLRDSKENFT
Sbjct: 181 QEDKSLLTKWMPLTEKYVEEWVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFT 240

Query: 281 GSMPENKSLPSQSGDELPSCNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAK 340
           GSMPENKSLPSQSGDELPSC DENL KTAQQEYFVPWHLPYHRAEVS TSASALASGLAK
Sbjct: 241 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 300

Query: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 352

[Close]

BLAST of MS003532 vs. ExPASy TrEMBL
Match: A0A6J1FLV5 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.3e-194
Identity = 345/393 (87.79%), Postives = 360/393 (91.60%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN SG +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENL KT QQEYFVPWHLPYHRAEVS TSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of MS003532 vs. ExPASy TrEMBL
Match: A0A6J1FG55 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.3e-194
Identity = 345/393 (87.79%), Postives = 360/393 (91.60%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN SG +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENL KT QQEYFVPWHLPYHRAEVS TSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 354 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 413

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 414 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of MS003532 vs. ExPASy TrEMBL
Match: A0A6J1JY21 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 343/393 (87.28%), Postives = 357/393 (90.84%), Query Frame = 0


Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F   SKIF LSSVV GS HH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSPCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q  CANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRIRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGPGSPR+RSPSSLALESIPEMNEN SG +L+DSK NFTGSMPEN  LPSQ G++  +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 300

Query: 301 CNDENLLKTAQQEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           CNDENL KT QQEYFVPWHLPYHRAEVS TSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of MS003532 vs. TAIR 10
Match: AT1G36310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 3.9e-133
Identity = 248/380 (65.26%), Postives = 282/380 (74.21%), Query Frame = 0


Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG----SPRIRSPSSLALESIPEMN-- 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG    SPR+R+    +LESIPE    
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 ------ENGSGIHLRDSKENFTGS--------MPENKSLPSQSGDELPSCNDENLLKTAQ 330
                 EN   I L    E+   +        +PE K+   +  DE         LK +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 390
           QEYFVPWHLPYHRAEVS  SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

[Close]

BLAST of MS003532 vs. TAIR 10
Match: AT1G36310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 3.9e-133
Identity = 248/380 (65.26%), Postives = 282/380 (74.21%), Query Frame = 0


Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSPCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG----SPRIRSPSSLALESIPEMN-- 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG    SPR+R+    +LESIPE    
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 ------ENGSGIHLRDSKENFTGS--------MPENKSLPSQSGDELPSCNDENLLKTAQ 330
                 EN   I L    E+   +        +PE K+   +  DE         LK +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSSTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 390
           QEYFVPWHLPYHRAEVS  SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

The following BLAST results are available for this feature:

BLAST of MS003532 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152525.1	8.6e-223	98.72	alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia] >XP...	[more]
XP_022152527.1	3.9e-199	98.58	alkylated DNA repair protein alkB homolog 8 isoform X2 [Momordica charantia]	[more]
XP_023518340.1	7.6e-195	87.28	alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo]	[more]
XP_022939521.1	1.1e-193	87.79	alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata]	[more]
XP_022939523.1	1.1e-193	87.79	alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata]	[more]

BLAST of MS003532 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94A09	5.5e-132	65.26	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX...	[more]
Q07G10	5.0e-56	38.51	Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk...	[more]
Q80Y20	3.6e-54	37.06	Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P...	[more]
A1A4L5	4.6e-54	35.78	Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2...	[more]
Q95K79	5.7e-52	36.31	Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL...	[more]

BLAST of MS003532 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DE66	4.2e-223	98.72	alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Momordica charantia OX...	[more]
A0A6J1DGH6	1.9e-199	98.58	alkylated DNA repair protein alkB homolog 8 isoform X2 OS=Momordica charantia OX...	[more]
A0A6J1FLV5	5.3e-194	87.79	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschat...	[more]
A0A6J1FG55	5.3e-194	87.79	alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1JY21	1.0e-192	87.28	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima ...	[more]

BLAST of MS003532 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36310.1	3.9e-133	65.26	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G36310.2	3.9e-133	65.26	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 124..214 e-value: 1.0E-14 score: 54.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 99..391 e-value: 8.6E-59 score: 200.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 273..301
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 266..301
None	No IPR available	PANTHER	PTHR13069:SF33	BNAA05G09210D PROTEIN	coord: 69..264
None	No IPR available	PANTHER	PTHR13069:SF33	BNAA05G09210D PROTEIN	coord: 308..385
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 69..264
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 308..385
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 123..219 e-value: 3.11217E-10 score: 55.1286
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 73..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003532.1	MS003532.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity