MS003452 (gene) Bitter gourd (TR) v1

BLAST of MS003452 vs. NCBI nr
Match: XP_022134288.1 (uncharacterized protein LOC111006587 [Momordica charantia] >XP_022134289.1 uncharacterized protein LOC111006587 [Momordica charantia])

HSP 1 Score: 1535.4 bits (3974), Expect = 0.0e+00
Identity = 749/749 (100.00%), Postives = 749/749 (100.00%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN
Sbjct: 1   MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
           ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH
Sbjct: 61  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 120

Query: 134 LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ 193
           LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ
Sbjct: 121 LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ 180

Query: 194 VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS 253
           VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS
Sbjct: 181 VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS 240

Query: 254 DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE 313
           DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE
Sbjct: 241 DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE 300

Query: 314 LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE 373
           LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE
Sbjct: 301 LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE 360

Query: 374 SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL 433
           SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL
Sbjct: 361 SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL 420

Query: 434 QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA 493
           QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA
Sbjct: 421 QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA 480

Query: 494 MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE 553
           MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE
Sbjct: 481 MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE 540

Query: 554 GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW 613
           GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW
Sbjct: 541 GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW 600

Query: 614 STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ 673
           STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ
Sbjct: 601 STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ 660

Query: 674 LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL 733
           LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL
Sbjct: 661 LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL 720

Query: 734 NEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           NEDYDSLLAGFDDLEVFRRQKESEDDNTS
Sbjct: 721 NEDYDSLLAGFDDLEVFRRQKESEDDNTS 749

BLAST of MS003452 vs. NCBI nr
Match: XP_022955213.1 (uncharacterized protein LOC111457243 isoform X1 [Cucurbita moschata] >XP_022955222.1 uncharacterized protein LOC111457243 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 682/764 (89.27%), Postives = 723/764 (94.63%), Query Frame = 0

Query: 1   FLFFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 60
           FLF  KVSVEIA M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI
Sbjct: 41  FLFSGKVSVEIAGMSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 100

Query: 61  HRIKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVD 120
           HRIKEHLAGHKGNASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVD
Sbjct: 101 HRIKEHLAGHKGNASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVD 160

Query: 121 AIPNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIP 180
           AI NHM++DSS+ LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIP
Sbjct: 161 AISNHMDMDSSLQLIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIP 220

Query: 181 NGGGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDI 240
           NGGGILDSNKVNNQVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDI
Sbjct: 221 NGGGILDSNKVNNQVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSAATGIIPPSYHDI 280

Query: 241 RGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESV 300
           RGWILKNSVEEVRSDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESV
Sbjct: 281 RGWILKNSVEEVRSDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESV 340

Query: 301 DASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAA 360
           DASGIMDSPD+LYELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAA
Sbjct: 341 DASGIMDSPDLLYELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAA 400

Query: 361 SCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATN 420
           SCVD+ILGDFGNIESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATN
Sbjct: 401 SCVDLILGDFGNIESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATN 460

Query: 421 FATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPL 480
           FATLNRMV+LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPL
Sbjct: 461 FATLNRMVDLKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPL 520

Query: 481 LRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHA 540
           LRVLRIVGSGKRPAMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q W+HPLHA
Sbjct: 521 LRVLRIVGSGKRPAMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWHHPLHA 580

Query: 541 AGFYLNPKFFYSVE-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRK 600
           AGFYLNPKFFYS+E G+MH EI+SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRK
Sbjct: 581 AGFYLNPKFFYSIEGGEMHGEIVSGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRK 640

Query: 601 MAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIE 660
           MAIRARETLLPAEWWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIE
Sbjct: 641 MAIRARETLLPAEWWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIE 700

Query: 661 HQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWT 720
           HQRLSDLVFVRFNLQL+QM AKANEQ  VDPLSFDGLGI+DDW+WR+DLCAED GNLEWT
Sbjct: 701 HQRLSDLVFVRFNLQLKQMAAKANEQYQVDPLSFDGLGIVDDWVWRRDLCAEDYGNLEWT 760

Query: 721 VLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           VLDNPPS S   LPL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 761 VLDNPPSGSTRLLPLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 804

BLAST of MS003452 vs. NCBI nr
Match: XP_023552367.1 (uncharacterized protein LOC111810055 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552375.1 uncharacterized protein LOC111810055 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552383.1 uncharacterized protein LOC111810055 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 681/764 (89.14%), Postives = 722/764 (94.50%), Query Frame = 0

Query: 1   FLFFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 60
           FLF  KVSVEIA M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI
Sbjct: 40  FLFSGKVSVEIAGMSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 99

Query: 61  HRIKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVD 120
           HRIKEHLAGHKGNASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVD
Sbjct: 100 HRIKEHLAGHKGNASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVD 159

Query: 121 AIPNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIP 180
           AI NHM++DSS+ LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIP
Sbjct: 160 AISNHMDMDSSLQLIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIP 219

Query: 181 NGGGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDI 240
           NGGGILDSNKVNNQVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDI
Sbjct: 220 NGGGILDSNKVNNQVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSAATGIIPPSYHDI 279

Query: 241 RGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESV 300
           RGWILKNSVEE+RSDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESV
Sbjct: 280 RGWILKNSVEELRSDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESV 339

Query: 301 DASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAA 360
           DASGIMDSPD+LYELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAA
Sbjct: 340 DASGIMDSPDLLYELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAA 399

Query: 361 SCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATN 420
           SCVD+ILGDFGNIESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATN
Sbjct: 400 SCVDLILGDFGNIESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATN 459

Query: 421 FATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPL 480
           FATLNRMV LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPL
Sbjct: 460 FATLNRMVELKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPL 519

Query: 481 LRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHA 540
           LRVLRIVGSGKRPAMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q W+HPLHA
Sbjct: 520 LRVLRIVGSGKRPAMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWHHPLHA 579

Query: 541 AGFYLNPKFFYSVE-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRK 600
           AGFYLNPKFFYS+E G+MH EI+SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRK
Sbjct: 580 AGFYLNPKFFYSIEGGEMHGEIVSGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRK 639

Query: 601 MAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIE 660
           MAIRARETLLPAEWWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIE
Sbjct: 640 MAIRARETLLPAEWWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIE 699

Query: 661 HQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWT 720
           HQRLSDLVFVRFNLQL+QM AKANEQ  VDPLSFDGLGI+DDW+WR+DLCAED GNLEWT
Sbjct: 700 HQRLSDLVFVRFNLQLKQMAAKANEQYQVDPLSFDGLGIVDDWVWRRDLCAEDYGNLEWT 759

Query: 721 VLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           VLDNPPS S   LPL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 760 VLDNPPSGSTRLLPLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 803

BLAST of MS003452 vs. NCBI nr
Match: KAG7015978.1 (hypothetical protein SDJN02_21082, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 666/761 (87.52%), Postives = 716/761 (94.09%), Query Frame = 0

Query: 3   FFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHR 62
           F W VSVEIAQM  GLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHR
Sbjct: 104 FLWTVSVEIAQMNFGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHR 163

Query: 63  IKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAI 122
           IKEHLAG KGNASTCHSVPPEVQN+MQESLDGVMMKK+KRQKLDEEMTNVNA+TGEVDAI
Sbjct: 164 IKEHLAGRKGNASTCHSVPPEVQNLMQESLDGVMMKKKKRQKLDEEMTNVNAITGEVDAI 223

Query: 123 PNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNG 182
            NHM++DSS HLIEV++PVE + GLL++HEEG+SNK+GRK+ SKGKSSCV+RDMIV+PNG
Sbjct: 224 TNHMDMDSSFHLIEVADPVETSSGLLVNHEEGSSNKVGRKKSSKGKSSCVDRDMIVLPNG 283

Query: 183 GGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRG 242
           GGILDSNK+NNQVHMA+GRFLFDIGASL+AVNSAYFQPMIESIVSAGTGIIPPSYHDIRG
Sbjct: 284 GGILDSNKLNNQVHMAIGRFLFDIGASLEAVNSAYFQPMIESIVSAGTGIIPPSYHDIRG 343

Query: 243 WILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDA 302
           WILKNSVEEVRSDFDR KATWG TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESVDA
Sbjct: 344 WILKNSVEEVRSDFDRCKATWGDTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESVDA 403

Query: 303 SGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASC 362
           SGIMDSPD+LYEL KKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAAS 
Sbjct: 404 SGIMDSPDLLYELFKKVVEQVGVKHVLQVITRFEENYAIAGRKLSDTYPTLYWTPCAASS 463

Query: 363 VDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFA 422
           VD+ILGD GNIE VNTV+E+ARSITRFVYNN+MVL++VRRYTYGNDI+EPC T+SATNFA
Sbjct: 464 VDLILGDIGNIEGVNTVIERARSITRFVYNNTMVLSMVRRYTYGNDIIEPCTTRSATNFA 523

Query: 423 TLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLR 482
           TLN+MV LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPLLR
Sbjct: 524 TLNQMVELKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPLLR 583

Query: 483 VLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAG 542
           VLRI GSGKRPAMGY+YAAIYNAKLAIKTELVDRERYMVYWNIIDQRWE HW+HPLHAAG
Sbjct: 584 VLRIAGSGKRPAMGYIYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEHHWHHPLHAAG 643

Query: 543 FYLNPKFFYSVEGDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAI 602
           FYLNPKFFYS+EG+MHSEI+SGMFDCIERLVSDTKIQD IIKE+NLYKNA  DLGRKMAI
Sbjct: 644 FYLNPKFFYSIEGEMHSEIMSGMFDCIERLVSDTKIQDSIIKEMNLYKNADKDLGRKMAI 703

Query: 603 RARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQR 662
           R RETLLPAEWWSTYG  C NL  LA RILSQTCS+ GF++NQILFDKLH+TRNHIEHQR
Sbjct: 704 RTRETLLPAEWWSTYGGRCKNLQSLATRILSQTCSSVGFQQNQILFDKLHDTRNHIEHQR 763

Query: 663 LSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLD 722
           LSDLVFVRFNLQL+QM AKANEQ PVDPLSFDGLGI+DDW+WR+DLCAEDCGNLEWT+LD
Sbjct: 764 LSDLVFVRFNLQLKQMAAKANEQHPVDPLSFDGLGIVDDWVWRRDLCAEDCGNLEWTILD 823

Query: 723 NPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           NPPS S M LP N+DYD L AGFDDLEVF+RQ+ESEDD  S
Sbjct: 824 NPPSSSTMLLPTNDDYDDLPAGFDDLEVFKRQRESEDDTIS 864

BLAST of MS003452 vs. NCBI nr
Match: XP_022991080.1 (uncharacterized protein LOC111487785 isoform X1 [Cucurbita maxima] >XP_022991081.1 uncharacterized protein LOC111487785 isoform X1 [Cucurbita maxima] >XP_022991082.1 uncharacterized protein LOC111487785 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1390.6 bits (3598), Expect = 0.0e+00
Identity = 679/764 (88.87%), Postives = 719/764 (94.11%), Query Frame = 0

Query: 1   FLFFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 60
           FLF  K SVEIA+M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI
Sbjct: 36  FLFSGKASVEIAEMSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 95

Query: 61  HRIKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVD 120
           HRIKEHLAGHKGNASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVD
Sbjct: 96  HRIKEHLAGHKGNASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVD 155

Query: 121 AIPNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIP 180
           AI NHM++DSS+ LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIP
Sbjct: 156 AISNHMDMDSSLQLIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIP 215

Query: 181 NGGGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDI 240
           NGGGILDSNKVNNQVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDI
Sbjct: 216 NGGGILDSNKVNNQVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSASTGIIPPSYHDI 275

Query: 241 RGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESV 300
           RGWILKNSVEEVRSDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESV
Sbjct: 276 RGWILKNSVEEVRSDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESV 335

Query: 301 DASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAA 360
           DASGIMDSPD+LYELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAA
Sbjct: 336 DASGIMDSPDLLYELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAA 395

Query: 361 SCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATN 420
           SCVD+ILGDFGNIESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATN
Sbjct: 396 SCVDLILGDFGNIESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATN 455

Query: 421 FATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPL 480
           FATLNRMV LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPL
Sbjct: 456 FATLNRMVELKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPL 515

Query: 481 LRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHA 540
           LRV RIVGSGKRPAMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q WNHPLHA
Sbjct: 516 LRVFRIVGSGKRPAMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWNHPLHA 575

Query: 541 AGFYLNPKFFYSVE-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRK 600
           AGFYLNPKFFYS+E G+MH EI SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRK
Sbjct: 576 AGFYLNPKFFYSIEGGEMHGEIASGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRK 635

Query: 601 MAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIE 660
           MAIRARETLLPAEWWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIE
Sbjct: 636 MAIRARETLLPAEWWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIE 695

Query: 661 HQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWT 720
           HQRLSDLVFVRFNLQL+QM AKANEQ  VDPLSFDGL I+DDW+WR+DLCAED GNLEWT
Sbjct: 696 HQRLSDLVFVRFNLQLKQMAAKANEQYQVDPLSFDGLSIVDDWVWRRDLCAEDYGNLEWT 755

Query: 721 VLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           VLD+PPS S   LPL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 756 VLDSPPSGSTRLLPLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 799

BLAST of MS003452 vs. ExPASy TrEMBL
Match: A0A6J1C1L7 (uncharacterized protein LOC111006587 OS=Momordica charantia OX=3673 GN=LOC111006587 PE=4 SV=1)

HSP 1 Score: 1535.4 bits (3974), Expect = 0.0e+00
Identity = 749/749 (100.00%), Postives = 749/749 (100.00%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN
Sbjct: 1   MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
           ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH
Sbjct: 61  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 120

Query: 134 LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ 193
           LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ
Sbjct: 121 LIEVSEPVEAPGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNNQ 180

Query: 194 VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS 253
           VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS
Sbjct: 181 VHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRS 240

Query: 254 DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE 313
           DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE
Sbjct: 241 DFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYE 300

Query: 314 LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE 373
           LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE
Sbjct: 301 LLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIE 360

Query: 374 SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL 433
           SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL
Sbjct: 361 SVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRCL 420

Query: 434 QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA 493
           QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA
Sbjct: 421 QTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRPA 480

Query: 494 MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE 553
           MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE
Sbjct: 481 MGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVE 540

Query: 554 GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW 613
           GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW
Sbjct: 541 GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWW 600

Query: 614 STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ 673
           STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ
Sbjct: 601 STYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFNLQ 660

Query: 674 LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL 733
           LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL
Sbjct: 661 LEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPLPL 720

Query: 734 NEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           NEDYDSLLAGFDDLEVFRRQKESEDDNTS
Sbjct: 721 NEDYDSLLAGFDDLEVFRRQKESEDDNTS 749

BLAST of MS003452 vs. ExPASy TrEMBL
Match: A0A6J1GSZ1 (uncharacterized protein LOC111457243 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457243 PE=4 SV=1)

HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 682/764 (89.27%), Postives = 723/764 (94.63%), Query Frame = 0

Query: 1   FLFFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 60
           FLF  KVSVEIA M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI
Sbjct: 41  FLFSGKVSVEIAGMSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 100

Query: 61  HRIKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVD 120
           HRIKEHLAGHKGNASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVD
Sbjct: 101 HRIKEHLAGHKGNASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVD 160

Query: 121 AIPNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIP 180
           AI NHM++DSS+ LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIP
Sbjct: 161 AISNHMDMDSSLQLIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIP 220

Query: 181 NGGGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDI 240
           NGGGILDSNKVNNQVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDI
Sbjct: 221 NGGGILDSNKVNNQVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSAATGIIPPSYHDI 280

Query: 241 RGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESV 300
           RGWILKNSVEEVRSDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESV
Sbjct: 281 RGWILKNSVEEVRSDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESV 340

Query: 301 DASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAA 360
           DASGIMDSPD+LYELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAA
Sbjct: 341 DASGIMDSPDLLYELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAA 400

Query: 361 SCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATN 420
           SCVD+ILGDFGNIESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATN
Sbjct: 401 SCVDLILGDFGNIESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATN 460

Query: 421 FATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPL 480
           FATLNRMV+LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPL
Sbjct: 461 FATLNRMVDLKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPL 520

Query: 481 LRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHA 540
           LRVLRIVGSGKRPAMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q W+HPLHA
Sbjct: 521 LRVLRIVGSGKRPAMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWHHPLHA 580

Query: 541 AGFYLNPKFFYSVE-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRK 600
           AGFYLNPKFFYS+E G+MH EI+SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRK
Sbjct: 581 AGFYLNPKFFYSIEGGEMHGEIVSGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRK 640

Query: 601 MAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIE 660
           MAIRARETLLPAEWWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIE
Sbjct: 641 MAIRARETLLPAEWWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIE 700

Query: 661 HQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWT 720
           HQRLSDLVFVRFNLQL+QM AKANEQ  VDPLSFDGLGI+DDW+WR+DLCAED GNLEWT
Sbjct: 701 HQRLSDLVFVRFNLQLKQMAAKANEQYQVDPLSFDGLGIVDDWVWRRDLCAEDYGNLEWT 760

Query: 721 VLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           VLDNPPS S   LPL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 761 VLDNPPSGSTRLLPLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 804

BLAST of MS003452 vs. ExPASy TrEMBL
Match: A0A6J1JKQ0 (uncharacterized protein LOC111487785 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487785 PE=4 SV=1)

HSP 1 Score: 1390.6 bits (3598), Expect = 0.0e+00
Identity = 679/764 (88.87%), Postives = 719/764 (94.11%), Query Frame = 0

Query: 1   FLFFWKVSVEIAQMTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 60
           FLF  K SVEIA+M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI
Sbjct: 36  FLFSGKASVEIAEMSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGI 95

Query: 61  HRIKEHLAGHKGNASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVD 120
           HRIKEHLAGHKGNASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVD
Sbjct: 96  HRIKEHLAGHKGNASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVD 155

Query: 121 AIPNHMEIDSSIHLIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIP 180
           AI NHM++DSS+ LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIP
Sbjct: 156 AISNHMDMDSSLQLIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIP 215

Query: 181 NGGGILDSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDI 240
           NGGGILDSNKVNNQVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDI
Sbjct: 216 NGGGILDSNKVNNQVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSASTGIIPPSYHDI 275

Query: 241 RGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESV 300
           RGWILKNSVEEVRSDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESV
Sbjct: 276 RGWILKNSVEEVRSDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESV 335

Query: 301 DASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAA 360
           DASGIMDSPD+LYELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAA
Sbjct: 336 DASGIMDSPDLLYELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAA 395

Query: 361 SCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATN 420
           SCVD+ILGDFGNIESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATN
Sbjct: 396 SCVDLILGDFGNIESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATN 455

Query: 421 FATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPL 480
           FATLNRMV LKRCLQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPL
Sbjct: 456 FATLNRMVELKRCLQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPL 515

Query: 481 LRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHA 540
           LRV RIVGSGKRPAMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q WNHPLHA
Sbjct: 516 LRVFRIVGSGKRPAMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWNHPLHA 575

Query: 541 AGFYLNPKFFYSVE-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRK 600
           AGFYLNPKFFYS+E G+MH EI SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRK
Sbjct: 576 AGFYLNPKFFYSIEGGEMHGEIASGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRK 635

Query: 601 MAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIE 660
           MAIRARETLLPAEWWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIE
Sbjct: 636 MAIRARETLLPAEWWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIE 695

Query: 661 HQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWT 720
           HQRLSDLVFVRFNLQL+QM AKANEQ  VDPLSFDGL I+DDW+WR+DLCAED GNLEWT
Sbjct: 696 HQRLSDLVFVRFNLQLKQMAAKANEQYQVDPLSFDGLSIVDDWVWRRDLCAEDYGNLEWT 755

Query: 721 VLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           VLD+PPS S   LPL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 756 VLDSPPSGSTRLLPLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 799

BLAST of MS003452 vs. ExPASy TrEMBL
Match: A0A6J1GT79 (uncharacterized protein LOC111457243 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457243 PE=4 SV=1)

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 672/751 (89.48%), Postives = 713/751 (94.94%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN
Sbjct: 1   MSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
           ASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVDAI NHM++DSS+ 
Sbjct: 61  ASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVDAISNHMDMDSSLQ 120

Query: 134 LIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNN 193
           LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIPNGGGILDSNKVNN
Sbjct: 121 LIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIPNGGGILDSNKVNN 180

Query: 194 QVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVR 253
           QVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDIRGWILKNSVEEVR
Sbjct: 181 QVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSAATGIIPPSYHDIRGWILKNSVEEVR 240

Query: 254 SDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLY 313
           SDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESVDASGIMDSPD+LY
Sbjct: 241 SDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESVDASGIMDSPDLLY 300

Query: 314 ELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNI 373
           ELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAASCVD+ILGDFGNI
Sbjct: 301 ELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAASCVDLILGDFGNI 360

Query: 374 ESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRC 433
           ESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATNFATLNRMV+LKRC
Sbjct: 361 ESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATNFATLNRMVDLKRC 420

Query: 434 LQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRP 493
           LQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPLLRVLRIVGSGKRP
Sbjct: 421 LQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPLLRVLRIVGSGKRP 480

Query: 494 AMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSV 553
           AMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q W+HPLHAAGFYLNPKFFYS+
Sbjct: 481 AMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWHHPLHAAGFYLNPKFFYSI 540

Query: 554 E-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAE 613
           E G+MH EI+SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRKMAIRARETLLPAE
Sbjct: 541 EGGEMHGEIVSGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRKMAIRARETLLPAE 600

Query: 614 WWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFN 673
           WWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIEHQRLSDLVFVRFN
Sbjct: 601 WWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIEHQRLSDLVFVRFN 660

Query: 674 LQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPL 733
           LQL+QM AKANEQ  VDPLSFDGLGI+DDW+WR+DLCAED GNLEWTVLDNPPS S   L
Sbjct: 661 LQLKQMAAKANEQYQVDPLSFDGLGIVDDWVWRRDLCAEDYGNLEWTVLDNPPSGSTRLL 720

Query: 734 PLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           PL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 721 PLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 751

BLAST of MS003452 vs. ExPASy TrEMBL
Match: A0A6J1JV56 (uncharacterized protein LOC111487785 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487785 PE=4 SV=1)

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 670/751 (89.21%), Postives = 709/751 (94.41%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M+SGLQ VPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN
Sbjct: 1   MSSGLQLVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
           ASTCHSVPPEVQ IMQESLDGV MKK+KRQKLDEEMTNVNAMTGEVDAI NHM++DSS+ 
Sbjct: 61  ASTCHSVPPEVQIIMQESLDGVTMKKKKRQKLDEEMTNVNAMTGEVDAISNHMDMDSSLQ 120

Query: 134 LIEVSEPVE-APGLLLSHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILDSNKVNN 193
           LIEV+EP+E +  LL++HEEG SNKLGRKRGSKGKSSCVER MIVIPNGGGILDSNKVNN
Sbjct: 121 LIEVAEPLETSSALLMNHEEGTSNKLGRKRGSKGKSSCVERGMIVIPNGGGILDSNKVNN 180

Query: 194 QVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVR 253
           QVHMAVGRFL+DIGASL+AVNSAYFQPMIESIVSA TGIIPPSYHDIRGWILKNSVEEVR
Sbjct: 181 QVHMAVGRFLYDIGASLEAVNSAYFQPMIESIVSASTGIIPPSYHDIRGWILKNSVEEVR 240

Query: 254 SDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLY 313
           SDFDR KATWG+TGCSVMVDQWRTE  RTMLIFLVY PEGTVFLESVDASGIMDSPD+LY
Sbjct: 241 SDFDRCKATWGKTGCSVMVDQWRTEAGRTMLIFLVYCPEGTVFLESVDASGIMDSPDLLY 300

Query: 314 ELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNI 373
           ELLKKVVEQVGVKHVLQVITR EENYAIAG+KLSDTYPTLYWTPCAASCVD+ILGDFGNI
Sbjct: 301 ELLKKVVEQVGVKHVLQVITRCEENYAIAGRKLSDTYPTLYWTPCAASCVDLILGDFGNI 360

Query: 374 ESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKSATNFATLNRMVNLKRC 433
           ESVNTV+EQARSITRFVYNNSMVLN+VRRYTYGNDILEPCAT+SATNFATLNRMV LKRC
Sbjct: 361 ESVNTVIEQARSITRFVYNNSMVLNMVRRYTYGNDILEPCATRSATNFATLNRMVELKRC 420

Query: 434 LQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVGSGKRP 493
           LQT+VTSQEWMDSPYSKRPGGLEMLDLISSESFWSSC+SI+RLTNPLLRV RIVGSGKRP
Sbjct: 421 LQTMVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCNSIIRLTNPLLRVFRIVGSGKRP 480

Query: 494 AMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSV 553
           AMGYVYAA+YNAKLAIKTELVDRERYMVYWNIIDQRW Q WNHPLHAAGFYLNPKFFYS+
Sbjct: 481 AMGYVYAAMYNAKLAIKTELVDRERYMVYWNIIDQRWGQQWNHPLHAAGFYLNPKFFYSI 540

Query: 554 E-GDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAE 613
           E G+MH EI SGMFDCIERLVSDTKIQDKIIKEIN YKNAVGDLGRKMAIRARETLLPAE
Sbjct: 541 EGGEMHGEIASGMFDCIERLVSDTKIQDKIIKEINSYKNAVGDLGRKMAIRARETLLPAE 600

Query: 614 WWSTYGAGCPNLARLAIRILSQTCSAEGFKKNQILFDKLHETRNHIEHQRLSDLVFVRFN 673
           WWSTY + CP L+RLAIRILSQTCS+ G K+NQILFDKLH+TRNHIEHQRLSDLVFVRFN
Sbjct: 601 WWSTYASSCPCLSRLAIRILSQTCSSVGLKQNQILFDKLHDTRNHIEHQRLSDLVFVRFN 660

Query: 674 LQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNLEWTVLDNPPSDSNMPL 733
           LQL+QM AKANEQ  VDPLSFDGL I+DDW+WR+DLCAED GNLEWTVLD+PPS S   L
Sbjct: 661 LQLKQMAAKANEQYQVDPLSFDGLSIVDDWVWRRDLCAEDYGNLEWTVLDSPPSGSTRLL 720

Query: 734 PLNEDYDSLLAGFDDLEVFRRQKESEDDNTS 763
           PL+ D D L+AGFDDLEVF+RQ+ESEDDN S
Sbjct: 721 PLHNDCDDLVAGFDDLEVFKRQRESEDDNIS 751

BLAST of MS003452 vs. TAIR 10
Match: AT3G22220.1 (hAT transposon superfamily )

HSP 1 Score: 726.9 bits (1875), Expect = 1.7e-209
Identity = 370/761 (48.62%), Postives = 516/761 (67.81%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M S L+PV +TPQK D AWKHC+++K GDRVQ++CLYC K+FKGGGI R+KEHLAG KG 
Sbjct: 1   MDSDLEPVALTPQKQDSAWKHCEVYKYGDRVQMRCLYCRKMFKGGGITRVKEHLAGKKGQ 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
            + C  VP EV+  +Q+ +DG + ++RKR+K   E   +        A     E+++ + 
Sbjct: 61  GTICDQVPDEVRLFLQQCIDGTVRRQRKRRKSSPEPLPI--------AYFPPCEVETQVA 120

Query: 134 L-IEVSEPVEAPG--LLLSHEEGASNK---LGRKRGSKGKSSC----VERDM-----IVI 193
              +V+   ++P   +++    G + +     RK  +  ++      V+RDM     + I
Sbjct: 121 ASSDVNNGFKSPSSDVVVGQSTGRTKQRTYRSRKNNAFERNDLANVEVDRDMDNLIPVAI 180

Query: 194 PNGGGIL--DSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSY 253
            +   I+   S +    VHMA+GRFLFDIGA  DA NS   QP I++IVS G G+  P++
Sbjct: 181 SSVKNIVHPTSKEREKTVHMAMGRFLFDIGADFDAANSVNVQPFIDAIVSGGFGVSIPTH 240

Query: 254 HDIRGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFL 313
            D+RGWILK+ VEEV+ + D  K  W RTGCSV+V +  +     +L FLVY PE  VFL
Sbjct: 241 EDLRGWILKSCVEEVKKEIDECKTLWKRTGCSVLVQELNSNEGPLILKFLVYCPEKVVFL 300

Query: 314 ESVDASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTP 373
           +SVDAS I+DS D LYELLK+VVE++G  +V+QVIT+ E++YA AGKKL D YP+LYW P
Sbjct: 301 KSVDASEILDSEDKLYELLKEVVEEIGDTNVVQVITKCEDHYAAAGKKLMDVYPSLYWVP 360

Query: 374 CAASCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKS 433
           CAA C+D +L +FG ++ +  ++EQAR++TR +YN+S VLN++R++T+GNDI++P  T S
Sbjct: 361 CAAHCIDKMLEEFGKMDWIREIIEQARTVTRIIYNHSGVLNLMRKFTFGNDIVQPVCTSS 420

Query: 434 ATNFATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLT 493
           ATNF T+ R+ +LK  LQ +VTS EW D  YSK  GGL M + I+ E FW + +    +T
Sbjct: 421 ATNFTTMGRIADLKPYLQAMVTSSEWNDCSYSKEAGGLAMTETINDEDFWKALTLANHIT 480

Query: 494 NPLLRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHP 553
            P+LRVLRIV S ++PAMGYVYAA+Y AK AIKT L  RE Y+VYW IID+ W Q    P
Sbjct: 481 APILRVLRIVCSERKPAMGYVYAAMYRAKEAIKTNLAHREEYIVYWKIIDRWWLQ---QP 540

Query: 554 LHAAGFYLNPKFFYSVEGDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLG 613
           L+AAGFYLNPKFFYS++ +M SEI   + DCIE+LV D  IQD +IK+IN YKNAVG  G
Sbjct: 541 LYAAGFYLNPKFFYSIDEEMRSEIHLAVVDCIEKLVPDVNIQDIVIKDINSYKNAVGIFG 600

Query: 614 RKMAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAE-GFKKNQILFDKLHETRN 673
           R +AIRAR+T+LPAEWWSTYG  C NL+R AIRILSQTCS+  G  +N     +++E++N
Sbjct: 601 RNLAIRARDTMLPAEWWSTYGESCLNLSRFAIRILSQTCSSSIGSVRNLTSISQIYESKN 660

Query: 674 HIEHQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNL 733
            IE QRL+DLVFV++N++L ++ ++++    VDPLS   + +L+DW+ R  +C E  G+ 
Sbjct: 661 SIERQRLNDLVFVQYNMRLRRIGSESSGDDTVDPLSHSNMEVLEDWVSRNQVCIEGNGSS 720

Query: 734 EWTVLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKES 757
           +W  L+       + + ++E  D L +GFDD E+F+ +KE+
Sbjct: 721 DWKSLEFIKRSEEVAVVIDETED-LGSGFDDAEIFKGEKEA 749

BLAST of MS003452 vs. TAIR 10
Match: AT3G22220.2 (hAT transposon superfamily )

HSP 1 Score: 726.9 bits (1875), Expect = 1.7e-209
Identity = 370/761 (48.62%), Postives = 516/761 (67.81%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M S L+PV +TPQK D AWKHC+++K GDRVQ++CLYC K+FKGGGI R+KEHLAG KG 
Sbjct: 1   MDSDLEPVALTPQKQDSAWKHCEVYKYGDRVQMRCLYCRKMFKGGGITRVKEHLAGKKGQ 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
            + C  VP EV+  +Q+ +DG + ++RKR+K   E   +        A     E+++ + 
Sbjct: 61  GTICDQVPDEVRLFLQQCIDGTVRRQRKRRKSSPEPLPI--------AYFPPCEVETQVA 120

Query: 134 L-IEVSEPVEAPG--LLLSHEEGASNK---LGRKRGSKGKSSC----VERDM-----IVI 193
              +V+   ++P   +++    G + +     RK  +  ++      V+RDM     + I
Sbjct: 121 ASSDVNNGFKSPSSDVVVGQSTGRTKQRTYRSRKNNAFERNDLANVEVDRDMDNLIPVAI 180

Query: 194 PNGGGIL--DSNKVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSY 253
            +   I+   S +    VHMA+GRFLFDIGA  DA NS   QP I++IVS G G+  P++
Sbjct: 181 SSVKNIVHPTSKEREKTVHMAMGRFLFDIGADFDAANSVNVQPFIDAIVSGGFGVSIPTH 240

Query: 254 HDIRGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFL 313
            D+RGWILK+ VEEV+ + D  K  W RTGCSV+V +  +     +L FLVY PE  VFL
Sbjct: 241 EDLRGWILKSCVEEVKKEIDECKTLWKRTGCSVLVQELNSNEGPLILKFLVYCPEKVVFL 300

Query: 314 ESVDASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTP 373
           +SVDAS I+DS D LYELLK+VVE++G  +V+QVIT+ E++YA AGKKL D YP+LYW P
Sbjct: 301 KSVDASEILDSEDKLYELLKEVVEEIGDTNVVQVITKCEDHYAAAGKKLMDVYPSLYWVP 360

Query: 374 CAASCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEPCATKS 433
           CAA C+D +L +FG ++ +  ++EQAR++TR +YN+S VLN++R++T+GNDI++P  T S
Sbjct: 361 CAAHCIDKMLEEFGKMDWIREIIEQARTVTRIIYNHSGVLNLMRKFTFGNDIVQPVCTSS 420

Query: 434 ATNFATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLT 493
           ATNF T+ R+ +LK  LQ +VTS EW D  YSK  GGL M + I+ E FW + +    +T
Sbjct: 421 ATNFTTMGRIADLKPYLQAMVTSSEWNDCSYSKEAGGLAMTETINDEDFWKALTLANHIT 480

Query: 494 NPLLRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQHWNHP 553
            P+LRVLRIV S ++PAMGYVYAA+Y AK AIKT L  RE Y+VYW IID+ W Q    P
Sbjct: 481 APILRVLRIVCSERKPAMGYVYAAMYRAKEAIKTNLAHREEYIVYWKIIDRWWLQ---QP 540

Query: 554 LHAAGFYLNPKFFYSVEGDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLG 613
           L+AAGFYLNPKFFYS++ +M SEI   + DCIE+LV D  IQD +IK+IN YKNAVG  G
Sbjct: 541 LYAAGFYLNPKFFYSIDEEMRSEIHLAVVDCIEKLVPDVNIQDIVIKDINSYKNAVGIFG 600

Query: 614 RKMAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAE-GFKKNQILFDKLHETRN 673
           R +AIRAR+T+LPAEWWSTYG  C NL+R AIRILSQTCS+  G  +N     +++E++N
Sbjct: 601 RNLAIRARDTMLPAEWWSTYGESCLNLSRFAIRILSQTCSSSIGSVRNLTSISQIYESKN 660

Query: 674 HIEHQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAEDCGNL 733
            IE QRL+DLVFV++N++L ++ ++++    VDPLS   + +L+DW+ R  +C E  G+ 
Sbjct: 661 SIERQRLNDLVFVQYNMRLRRIGSESSGDDTVDPLSHSNMEVLEDWVSRNQVCIEGNGSS 720

Query: 734 EWTVLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKES 757
           +W  L+       + + ++E  D L +GFDD E+F+ +KE+
Sbjct: 721 DWKSLEFIKRSEEVAVVIDETED-LGSGFDDAEIFKGEKEA 749

BLAST of MS003452 vs. TAIR 10
Match: AT4G15020.1 (hAT transposon superfamily )

HSP 1 Score: 704.5 bits (1817), Expect = 9.2e-203
Identity = 361/769 (46.94%), Postives = 518/769 (67.36%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M + L+PV +TPQK D AWKHC+++K GDR+Q++CLYC K+FKGGGI R+KEHLAG KG 
Sbjct: 1   MDAELEPVALTPQKQDNAWKHCEIYKYGDRLQMRCLYCRKMFKGGGITRVKEHLAGKKGQ 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
            + C  VP +V+  +Q+ +DG + ++RKR K   E  +V ++          +E D  + 
Sbjct: 61  GTICDQVPEDVRLFLQQCIDGTVRRQRKRHKSSSEPLSVASLP--------PIEGDMMVV 120

Query: 134 LIEVSEPVEAPG---LLLSHEEGASNKLGRKRGSKGKSSCVE------------RDM--- 193
             +V++  ++PG   +++ +E   S +  ++R  + K +  E            RDM   
Sbjct: 121 QPDVNDGFKSPGSSDVVVQNESLLSGRT-KQRTYRSKKNAFENGSASNNVDLIGRDMDNL 180

Query: 194 --IVIPNGGGILDSN--KVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGI 253
             + I +   I+  +     N +HMA+GRFLF IGA  DAVNS  FQPMI++I S G G+
Sbjct: 181 IPVAISSVKNIVHPSFRDRENTIHMAIGRFLFGIGADFDAVNSVNFQPMIDAIASGGFGV 240

Query: 254 IPPSYHDIRGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPE 313
             P++ D+RGWILKN VEE+  + D  KA W RTGCS++V++  ++    +L FLVY PE
Sbjct: 241 SAPTHDDLRGWILKNCVEEMAKEIDECKAMWKRTGCSILVEELNSDKGFKVLNFLVYCPE 300

Query: 314 GTVFLESVDASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPT 373
             VFL+SVDAS ++ S D L+ELL ++VE+VG  +V+QVIT+ ++ Y  AGK+L   YP+
Sbjct: 301 KVVFLKSVDASEVLSSADKLFELLSELVEEVGSTNVVQVITKCDDYYVDAGKRLMLVYPS 360

Query: 374 LYWTPCAASCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEP 433
           LYW PCAA C+D +L +FG +  ++  +EQA++ITRFVYN+S VLN++ ++T GNDIL P
Sbjct: 361 LYWVPCAAHCIDQMLEEFGKLGWISETIEQAQAITRFVYNHSGVLNLMWKFTSGNDILLP 420

Query: 434 CATKSATNFATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSS 493
             + SATNFATL R+  LK  LQ +VTS EW +  YS+ P GL +++ ++ E+FW + + 
Sbjct: 421 AFSSSATNFATLGRIAELKSNLQAMVTSAEWNECSYSEEPSGL-VMNALTDEAFWKAVAL 480

Query: 494 IVRLTNPLLRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQ 553
           +  LT+PLLR LRIV S KRPAMGYVYAA+Y AK AIKT LV+RE Y++YW IID+ WEQ
Sbjct: 481 VNHLTSPLLRALRIVCSEKRPAMGYVYAALYRAKDAIKTHLVNREDYIIYWKIIDRWWEQ 540

Query: 554 HWNHPLHAAGFYLNPKFFYSVEGDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNA 613
             + PL AAGF+LNPK FY+   ++ SE++  + DCIERLV D KIQDKIIKE+  YK A
Sbjct: 541 QQHIPLLAAGFFLNPKLFYNTNEEIRSELILSVLDCIERLVPDDKIQDKIIKELTSYKTA 600

Query: 614 VGDLGRKMAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAE-GFKKNQILFDKL 673
            G  GR +AIRAR+T+LPAEWWSTYG  C NL+R AIRILSQTCS+    ++NQI  + +
Sbjct: 601 GGVFGRNLAIRARDTMLPAEWWSTYGESCLNLSRFAIRILSQTCSSSVSCRRNQIPVEHI 660

Query: 674 HETRNHIEHQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAE 733
           ++++N IE +RLSDLVFV++N++L Q+   + +   +DPLS + + +L +W+     C E
Sbjct: 661 YQSKNSIEQKRLSDLVFVQYNMRLRQLGPGSGDD-TLDPLSHNRIDVLKEWVSGDQACVE 720

Query: 734 DCGNLEWTVLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDD 760
             G+ +W  L++     N   P+ +D + L +GFDD+E+F+ +KE  D+
Sbjct: 721 GNGSADWKSLES--IHRNQVAPIIDDTEDLGSGFDDIEIFKVEKEVRDE 756

BLAST of MS003452 vs. TAIR 10
Match: AT4G15020.2 (hAT transposon superfamily )

HSP 1 Score: 704.5 bits (1817), Expect = 9.2e-203
Identity = 361/769 (46.94%), Postives = 518/769 (67.36%), Query Frame = 0

Query: 14  MTSGLQPVPITPQKHDPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGN 73
           M + L+PV +TPQK D AWKHC+++K GDR+Q++CLYC K+FKGGGI R+KEHLAG KG 
Sbjct: 1   MDAELEPVALTPQKQDNAWKHCEIYKYGDRLQMRCLYCRKMFKGGGITRVKEHLAGKKGQ 60

Query: 74  ASTCHSVPPEVQNIMQESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIH 133
            + C  VP +V+  +Q+ +DG + ++RKR K   E  +V ++          +E D  + 
Sbjct: 61  GTICDQVPEDVRLFLQQCIDGTVRRQRKRHKSSSEPLSVASLP--------PIEGDMMVV 120

Query: 134 LIEVSEPVEAPG---LLLSHEEGASNKLGRKRGSKGKSSCVE------------RDM--- 193
             +V++  ++PG   +++ +E   S +  ++R  + K +  E            RDM   
Sbjct: 121 QPDVNDGFKSPGSSDVVVQNESLLSGRT-KQRTYRSKKNAFENGSASNNVDLIGRDMDNL 180

Query: 194 --IVIPNGGGILDSN--KVNNQVHMAVGRFLFDIGASLDAVNSAYFQPMIESIVSAGTGI 253
             + I +   I+  +     N +HMA+GRFLF IGA  DAVNS  FQPMI++I S G G+
Sbjct: 181 IPVAISSVKNIVHPSFRDRENTIHMAIGRFLFGIGADFDAVNSVNFQPMIDAIASGGFGV 240

Query: 254 IPPSYHDIRGWILKNSVEEVRSDFDRFKATWGRTGCSVMVDQWRTETCRTMLIFLVYSPE 313
             P++ D+RGWILKN VEE+  + D  KA W RTGCS++V++  ++    +L FLVY PE
Sbjct: 241 SAPTHDDLRGWILKNCVEEMAKEIDECKAMWKRTGCSILVEELNSDKGFKVLNFLVYCPE 300

Query: 314 GTVFLESVDASGIMDSPDMLYELLKKVVEQVGVKHVLQVITRSEENYAIAGKKLSDTYPT 373
             VFL+SVDAS ++ S D L+ELL ++VE+VG  +V+QVIT+ ++ Y  AGK+L   YP+
Sbjct: 301 KVVFLKSVDASEVLSSADKLFELLSELVEEVGSTNVVQVITKCDDYYVDAGKRLMLVYPS 360

Query: 374 LYWTPCAASCVDMILGDFGNIESVNTVLEQARSITRFVYNNSMVLNIVRRYTYGNDILEP 433
           LYW PCAA C+D +L +FG +  ++  +EQA++ITRFVYN+S VLN++ ++T GNDIL P
Sbjct: 361 LYWVPCAAHCIDQMLEEFGKLGWISETIEQAQAITRFVYNHSGVLNLMWKFTSGNDILLP 420

Query: 434 CATKSATNFATLNRMVNLKRCLQTVVTSQEWMDSPYSKRPGGLEMLDLISSESFWSSCSS 493
             + SATNFATL R+  LK  LQ +VTS EW +  YS+ P GL +++ ++ E+FW + + 
Sbjct: 421 AFSSSATNFATLGRIAELKSNLQAMVTSAEWNECSYSEEPSGL-VMNALTDEAFWKAVAL 480

Query: 494 IVRLTNPLLRVLRIVGSGKRPAMGYVYAAIYNAKLAIKTELVDRERYMVYWNIIDQRWEQ 553
           +  LT+PLLR LRIV S KRPAMGYVYAA+Y AK AIKT LV+RE Y++YW IID+ WEQ
Sbjct: 481 VNHLTSPLLRALRIVCSEKRPAMGYVYAALYRAKDAIKTHLVNREDYIIYWKIIDRWWEQ 540

Query: 554 HWNHPLHAAGFYLNPKFFYSVEGDMHSEILSGMFDCIERLVSDTKIQDKIIKEINLYKNA 613
             + PL AAGF+LNPK FY+   ++ SE++  + DCIERLV D KIQDKIIKE+  YK A
Sbjct: 541 QQHIPLLAAGFFLNPKLFYNTNEEIRSELILSVLDCIERLVPDDKIQDKIIKELTSYKTA 600

Query: 614 VGDLGRKMAIRARETLLPAEWWSTYGAGCPNLARLAIRILSQTCSAE-GFKKNQILFDKL 673
            G  GR +AIRAR+T+LPAEWWSTYG  C NL+R AIRILSQTCS+    ++NQI  + +
Sbjct: 601 GGVFGRNLAIRARDTMLPAEWWSTYGESCLNLSRFAIRILSQTCSSSVSCRRNQIPVEHI 660

Query: 674 HETRNHIEHQRLSDLVFVRFNLQLEQMVAKANEQCPVDPLSFDGLGILDDWIWRKDLCAE 733
           ++++N IE +RLSDLVFV++N++L Q+   + +   +DPLS + + +L +W+     C E
Sbjct: 661 YQSKNSIEQKRLSDLVFVQYNMRLRQLGPGSGDD-TLDPLSHNRIDVLKEWVSGDQACVE 720

Query: 734 DCGNLEWTVLDNPPSDSNMPLPLNEDYDSLLAGFDDLEVFRRQKESEDD 760
             G+ +W  L++     N   P+ +D + L +GFDD+E+F+ +KE  D+
Sbjct: 721 GNGSADWKSLES--IHRNQVAPIIDDTEDLGSGFDDIEIFKVEKEVRDE 756

BLAST of MS003452 vs. TAIR 10
Match: AT3G17450.1 (hAT dimerisation domain-containing protein )

HSP 1 Score: 347.4 bits (890), Expect = 2.8e-95
Identity = 208/690 (30.14%), Postives = 355/690 (51.45%), Query Frame = 0

Query: 29  DPAWKHCQMFKNGDRVQLKCLYCHKLFKGGGIHRIKEHLAGHKGNASTCHSVPPEVQNIM 88
           DP W+H  + ++  + ++KC YC+K+   GGI+R K+HLA   G  + C + P EV   +
Sbjct: 133 DPGWEH-GIAQDERKKKVKCNYCNKIV-SGGINRFKQHLARIPGEVAPCKTAPEEVYVKI 192

Query: 89  QESLDGVMMKKRKRQKLDEEMTNVNAMTGEVDAIPNHMEIDSSIHLIEVSEPVEAPGLLL 148
           +E++      KR+ +  D+EM  +   T   D        D   +       +   G   
Sbjct: 193 KENMKWHRAGKRQNRP-DDEMGALTFRTVSQDPDQEEDREDHDFYPTSQDRLMLGNGRFS 252

Query: 149 SHEEGASNKLGRKRGSKGKSSCVERDMIVIPNGGGILD-----SNKV--NNQVHMAVGRF 208
             +  + +    +  S+ K+          P+           SN+V     V  ++ +F
Sbjct: 253 KDKRKSFDSTNMRSVSEAKTKRARMIPFQSPSSSKQRKLYSSCSNRVVSRKDVTSSISKF 312

Query: 209 LFDIGASLDAVNSAYFQPMIESIVSAGTGIIPPSYHDIRGWILKNSVEEVRSDFDRFKAT 268
           L  +G   +A NS YFQ MIE I   G G + PS     G +L+  +  ++S    ++++
Sbjct: 313 LHHVGVPTEAANSLYFQKMIELIGMYGEGFVVPSSQLFSGRLLQEEMSTIKSYLREYRSS 372

Query: 269 WGRTGCSVMVDQWRTETCRTMLIFLVYSPEGTVFLESVDASGIMDSPDMLYELLKKVVEQ 328
           W  TGCS+M D W     + M+ FLV  P G  F  S+DA+ I++    L++ L K+V+ 
Sbjct: 373 WVVTGCSIMADTWTNTEGKKMISFLVSCPRGVYFHSSIDATDIVEDALSLFKCLDKLVDD 432

Query: 329 VGVKHVLQVITRSEENYAIAGKKLSDTYPTLYWTPCAASCVDMILGDFGNIESVNTVLEQ 388
           +G ++V+QVIT++   +  AGK L +    LYWTPCA  C +++L DF  +E V+  LE+
Sbjct: 433 IGEENVVQVITQNTAIFRSAGKLLEEKRKNLYWTPCAIHCTELVLEDFSKLEFVSECLEK 492

Query: 389 ARSITRFVYNNSMVLNIVR-RYTYGNDILEPCATKSATNFATLNRMVNLKRCLQTVVTSQ 448
           A+ ITRF+YN + +LN+++  +T G D+L P   + A+ F TL  +++ K  L+ +  S 
Sbjct: 493 AQRITRFIYNQTWLLNLMKNEFTQGLDLLRPAVMRHASGFTTLQSLMDHKASLRGLFQSD 552

Query: 449 EW-MDSPYSKRPGGLEMLDLISSESFWSSCSSIVRLTNPLLRVLRIVG-SGKRPAMGYVY 508
            W +    +K   G E+  ++ S  FW     +++  +P+++V+ ++   G R +M Y Y
Sbjct: 553 GWILSQTAAKSEEGREVEKMVLSAVFWKKVQYVLKSVDPVMQVIHMINDGGDRLSMPYAY 612

Query: 509 AAIYNAKLAIKT-ELVDRERYMVYWNIIDQRWEQHWNHPLHAAGFYLNPKFFYSVEGDMH 568
             +  AK+AIK+    D  +Y  +W +I+ RW   ++HPL+ A ++ NP + Y  +    
Sbjct: 613 GYMCCAKMAIKSIHSDDARKYGPFWRVIEYRWNPLFHHPLYVAAYFFNPAYKYRPDFMAQ 672

Query: 569 SEILSGMFDCIERLVSDTKIQDKIIKEINLYKNAVGDLGRKMAIRARETLLPAEWWSTYG 628
           SE++ G+ +CI RL  D   +   + +I  Y  A  D G  +AI  R  L P+ WW  +G
Sbjct: 673 SEVVRGVNECIVRLEPDNTRRITALMQIPDYTCAKADFGTDIAIGTRTELDPSAWWQQHG 732

Query: 629 AGCPNLARLAIRILSQTCSAEGFKKNQILFDKLH-ETRNHIEHQRLSDLVFVRFNLQLEQ 688
             C  L R+A+RILS TCS+ G +    ++D+++ + ++    +   DL +V +NL+L +
Sbjct: 733 ISCLELQRVAVRILSHTCSSVGCEPKWSVYDQVNSQCQSQFGKKSTKDLTYVHYNLRLRE 792

Query: 689 MVAKAN---EQCPVDPLSFDGLG-ILDDWI 703
              K     E  P   L+   L  +L DW+
Sbjct: 793 KQLKQRLHYEDEPPPTLNHALLDRLLPDWL 819