MS003439 (gene) Bitter gourd (TR) v1

BLAST of MS003439 vs. NCBI nr
Match: XP_022134390.1 (delta-1-pyrroline-5-carboxylate synthase [Momordica charantia])

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 698/715 (97.62%), Postives = 700/715 (97.90%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR
Sbjct: 315 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI
Sbjct: 555 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHIYQHG             AHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF
Sbjct: 615 DHIYQHG------------SAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 717

BLAST of MS003439 vs. NCBI nr
Match: XP_004138450.1 (delta-1-pyrroline-5-carboxylate synthase [Cucumis sativus] >KGN45747.1 hypothetical protein Csa_005734 [Cucumis sativus])

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 658/715 (92.03%), Postives = 687/715 (96.08%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLL+LKV+PIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLTLKVVPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAP NI+KVLKGDRIGTLFHQDAHLW PQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPGNILKVLKGDRIGTLFHQDAHLWTPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIST+NEADV EAQQ+GYEKALVSRLA+KPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISTENEADVAEAQQSGYEKALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+++GG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITEAIPESIGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEM+KRIVLDAKIDYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMSKRIVLDAKIDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TGGLNEL+VNLRIEGVTL GGPRASSLLNIPEA+TFHHEYNSL CT+EIVDDVF AI
Sbjct: 555 LVQTGGLNELVVNLRIEGVTLNGGPRASSLLNIPEARTFHHEYNSLACTLEIVDDVFDAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTEDHEVA+VFLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDHEVAEVFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVVDGDKGVVYTH+DIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVDGDKGVVYTHRDIKIES 717

BLAST of MS003439 vs. NCBI nr
Match: XP_038886603.1 (delta-1-pyrroline-5-carboxylate synthase [Benincasa hispida])

HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 661/714 (92.58%), Postives = 685/714 (95.94%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAP NI+KVLKGDRIGTLFHQDAHLWAPQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPGNILKVLKGDRIGTLFHQDAHLWAPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIST+NEADV EAQQ+GYEKALVSRLALKPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISTENEADVAEAQQSGYEKALVSRLALKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSD LILEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDRLILEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP++VGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITEAIPESVGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKVDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV  GGLNEL+VNLRIEGV L GGPRASSLLNIPEA+TFHHEYNSL CT+EIVDDVFAAI
Sbjct: 555 LVQLGGLNELVVNLRIEGVILNGGPRASSLLNIPEARTFHHEYNSLACTLEIVDDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTEDHEVA+VFLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDHEVAEVFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIE 727
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVVDGDKGVVYTH+DIKIE
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVDGDKGVVYTHRDIKIE 716

BLAST of MS003439 vs. NCBI nr
Match: XP_023546433.1 (delta-1-pyrroline-5-carboxylate synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 652/715 (91.19%), Postives = 686/715 (95.94%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVVKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQ+DV+SAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQVDVSSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAA GAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAAVGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAPENI+KVLKGDRIGTLFHQDAHLWAPQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPENILKVLKGDRIGTLFHQDAHLWAPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLD+ADALE NEKLI T+NEADV EAQQ GYE+ALVSRLA+KPGKISSLANSIR
Sbjct: 315 QERRKILLDVADALETNEKLIRTENEADVAEAQQTGYERALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIG VLK+TEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGRVLKKTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHK+ITAAIPD+VGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKIITAAIPDSVGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKVDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TG LNEL+VNLRIEGVTLYGGP+ASSLLNIPEA+TFHHEYNSLTCTIEIVDDVFAAI
Sbjct: 555 LVHTGMLNELVVNLRIEGVTLYGGPKASSLLNIPEARTFHHEYNSLTCTIEIVDDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED +VA++FLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDDKVAEIFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVV+GDKGV+YTHKDIKI+S
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVNGDKGVIYTHKDIKIDS 717

BLAST of MS003439 vs. NCBI nr
Match: XP_008441400.1 (PREDICTED: delta-1-pyrroline-5-carboxylate synthase [Cucumis melo])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 655/715 (91.61%), Postives = 683/715 (95.52%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLL+LKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLALKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGY P NI+KVLKGDRIGTLFHQDAHLWAPQK+ GARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYTPGNILKVLKGDRIGTLFHQDAHLWAPQKDDGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIST+NEADV EAQQ+GYEKALVSRLA+KPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISTENEADVAEAQQSGYEKALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+++GG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITGAIPESIGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAK+DYPAACNAMETLLVH D
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKVDYPAACNAMETLLVHND 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TGGLNEL+VNLRIEGVTL GGPRASSLLNIPEA+TFHHEYNSL CT+EIVDDV  AI
Sbjct: 555 LVQTGGLNELVVNLRIEGVTLNGGPRASSLLNIPEARTFHHEYNSLACTLEIVDDVLDAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED EVA+VFLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDREVAEVFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVVDGDKGVVYTH+DIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVDGDKGVVYTHRDIKIES 717

BLAST of MS003439 vs. ExPASy Swiss-Prot
Match: O04015 (Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 592/708 (83.62%), Postives = 651/708 (91.95%), Query Frame = 0

Query: 16  IIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRKLVN 75
           IIKVGTAVVTR DGRLALGRLGALCEQI ELNSQG+E+ILV+SGAVG+GRQRLRYRKLVN
Sbjct: 18  IIKVGTAVVTRADGRLALGRLGALCEQIHELNSQGFEVILVTSGAVGVGRQRLRYRKLVN 77

Query: 76  SSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFRRQL 135
           SSFADLQKPQI+LDGKACAAVGQN L+ALYDTLFSQLDVTSAQLLVTDNDFRD +FR+QL
Sbjct: 78  SSFADLQKPQIELDGKACAAVGQNGLLALYDTLFSQLDVTSAQLLVTDNDFRDPEFRKQL 137

Query: 136 NDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVLLSD 195
            +TV+SLL+LKVIPIFNENDAVSTRKAPYED+SGIFWDNDSLAALLALELKADLLVLLSD
Sbjct: 138 TETVESLLNLKVIPIFNENDAVSTRKAPYEDASGIFWDNDSLAALLALELKADLLVLLSD 197

Query: 196 VAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGIPVV 255
           V GLYSGPPSDP SKLIHTYIKE  +G ITFGDKSRVGRGGMTAKVKAA  AA+AGIPVV
Sbjct: 198 VEGLYSGPPSDPQSKLIHTYIKEMFEGLITFGDKSRVGRGGMTAKVKAAVYAAHAGIPVV 257

Query: 256 ITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISSQGR 315
           ITSGYA  NIIKVL+G+RIGTLFH+DA  WAP  +VGARDMAVAARESSRRLQA+S Q R
Sbjct: 258 ITSGYATNNIIKVLQGERIGTLFHRDAQKWAPVGDVGARDMAVAARESSRRLQAMSPQDR 317

Query: 316 RKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIRTLA 375
            KILLD+ADALEANEKLI  +NEAD+  AQQAGYEK+L+SRLALK GKISSLA SIR LA
Sbjct: 318 SKILLDVADALEANEKLIRIENEADLAAAQQAGYEKSLISRLALKSGKISSLAKSIRVLA 377

Query: 376 NMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLLLKG 435
           NME+PIGHVLKRTE++DGL+LEKTSSPLGVLLI+FESRPDALVQIASLA+RSGNGL+LKG
Sbjct: 378 NMEEPIGHVLKRTEITDGLVLEKTSSPLGVLLIIFESRPDALVQIASLAIRSGNGLVLKG 437

Query: 436 GKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVS 495
           GKEA+RSNAILHKVIT+AIP+ VG  LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVS
Sbjct: 438 GKEAKRSNAILHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVS 497

Query: 496 QIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKDLVP 555
           QIK STKIPVLGHADGICHVYVDKSA+++MAK++VLDAK DYPAACNAMETLLVHKDLV 
Sbjct: 498 QIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAACNAMETLLVHKDLVQ 557

Query: 556 TGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAIDHI 615
            G L+ELIV L+I+GV ++GGPRASSLL+IPEA++ HHEY+SL CTIEIVDDV+AAIDHI
Sbjct: 558 NGCLDELIVELQIKGVVIHGGPRASSLLHIPEARSLHHEYSSLACTIEIVDDVYAAIDHI 617

Query: 616 YQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARFGLG 675
           ++HG             AHTD I+TEDHEVA++FLRQVDS++V HNASTRF DGARFGLG
Sbjct: 618 HRHG------------SAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFGLG 677

Query: 676 AEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDI 724
           AEVGISTSRIHARGPVGVEGLLTTRWI RG+GQVVDGDKG+VYTHKD+
Sbjct: 678 AEVGISTSRIHARGPVGVEGLLTTRWIARGSGQVVDGDKGIVYTHKDL 713

BLAST of MS003439 vs. ExPASy Swiss-Prot
Match: Q96480 (Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2 PE=2 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 578/715 (80.84%), Postives = 641/715 (89.65%), Query Frame = 0

Query: 9   VLRCRAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRL 68
           V   +  I+KVGTAVVTR DGRLALGRLGALCEQ++ELNSQGYE+ILV+SGAVG+GRQRL
Sbjct: 11  VKNVKRLIVKVGTAVVTRADGRLALGRLGALCEQLQELNSQGYEVILVTSGAVGVGRQRL 70

Query: 69  RYRKLVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRD 128
           RYRKL+NSSF DLQKPQ +LDGKACAAVGQN LMALYD+LFSQLDVTSAQLLVTDNDFRD
Sbjct: 71  RYRKLLNSSFLDLQKPQTELDGKACAAVGQNGLMALYDSLFSQLDVTSAQLLVTDNDFRD 130

Query: 129 KDFRRQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKAD 188
            DFRRQLNDTV SLLSLKVIPIFNENDA+STR+APYEDSSGIFWDNDSLAALLALELKAD
Sbjct: 131 PDFRRQLNDTVNSLLSLKVIPIFNENDAISTRRAPYEDSSGIFWDNDSLAALLALELKAD 190

Query: 189 LLVLLSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAA 248
           LLVLLSDV GLYSGPP DP SKLI+TYIKE H+  ITFGDKSRVGRGGMTAKVKAA  AA
Sbjct: 191 LLVLLSDVDGLYSGPPRDPDSKLIYTYIKEIHERVITFGDKSRVGRGGMTAKVKAAMYAA 250

Query: 249 YAGIPVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQ 308
           YAGIPVVITSG+A +NIIKVL G+RIGTLFH DA+ WA   E  AR+MAVAAR  SRRLQ
Sbjct: 251 YAGIPVVITSGFATDNIIKVLHGERIGTLFHCDANKWASIGETDAREMAVAARACSRRLQ 310

Query: 309 AISSQGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLA 368
           A+SSQ R KIL DIADALEANEK I  +NEADV  AQQAGYEK+L+SRLAL PGKISSLA
Sbjct: 311 ALSSQERSKILQDIADALEANEKAILAENEADVVAAQQAGYEKSLISRLALNPGKISSLA 370

Query: 369 NSIRTLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSG 428
           NS+R L+NM++P+GH LKRTE++DG ILEK+SSPLGV+LI+FESRPDALVQIASLAVRSG
Sbjct: 371 NSVRVLSNMDEPLGHTLKRTEIADGFILEKSSSPLGVVLIIFESRPDALVQIASLAVRSG 430

Query: 429 NGLLLKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPR 488
           NGL+LKGGKEA+RSNAILHKVIT+AIP +VG  LIGLVTSREEIP+LLKLDDVIDLVIPR
Sbjct: 431 NGLMLKGGKEAKRSNAILHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDVIDLVIPR 490

Query: 489 GSNKLVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLL 548
           GSNKLVSQIK+STKIPVLGHADGICHVYVDKSAD++MAKRI +DAKIDYPAACNAMETLL
Sbjct: 491 GSNKLVSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAACNAMETLL 550

Query: 549 VHKDLVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDV 608
           VHKDL   GGLN+LIV L+ +GV+LYGGP+ASSLL IPEA+TF HEY+SL CT+E+V+DV
Sbjct: 551 VHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMIPEARTFRHEYSSLACTVEVVEDV 610

Query: 609 FAAIDHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCD 668
           +AAIDHI+QHG             AHTD I+TED EVA+VFLRQVDSAAVFHNASTRF D
Sbjct: 611 YAAIDHIHQHG------------SAHTDSIITEDQEVAEVFLRQVDSAAVFHNASTRFSD 670

Query: 669 GARFGLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDI 724
           G RFGLGAEVGIST RIHARGPVGVEGLLTT+W+ RG+GQ+VDGDK +VY+HKD+
Sbjct: 671 GFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLARGSGQIVDGDKSIVYSHKDL 713

BLAST of MS003439 vs. ExPASy Swiss-Prot
Match: P54887 (Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5CSA PE=1 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 559/715 (78.18%), Postives = 636/715 (88.95%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGALCEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ +LDGKACA VGQ+SLMA Y+T+F QLDVT+AQLLV D+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QLN+TVKS+L L+VIPIFNENDA+STR+APY+DSSGIFWDNDSLAALLALELKADLL+L
Sbjct: 135 KQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSDP+SKLIHT++KE+HQ EITFGDKSR+GRGGMTAKVKAA  AAYAGI
Sbjct: 195 LSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGY+ ENI KVL+G R+GTLFHQDA LWAP  +  ARDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R+KILLDIADALEAN   I  +NE DV  AQ+AG E+++V+RL + PGKISSLA S+R
Sbjct: 315 EDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA+MEDPIG VLK+TEV+DGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 KLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LV+QIK++TKIPVLGHADGICHVYVDK+ D +MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L     LNELI  L+  GVTLYGGPRAS +LNIPEA++F+HEY +  CT+E+V+DV+ AI
Sbjct: 555 LEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI++HG             AHTDCIVTEDHEVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHRHG------------SAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVG+ST RIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTH+DI I++
Sbjct: 675 GLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA 717

BLAST of MS003439 vs. ExPASy Swiss-Prot
Match: O65361 (Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=3544 GN=P5CS PE=2 SV=1)

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 562/712 (78.93%), Postives = 629/712 (88.34%), Query Frame = 0

Query: 16  IIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRKLVN 75
           ++KVGTAVVTR+DGRLALGRLG+LCEQ+KELNS GYE+ILV+SGAV  GRQRLR+RKLVN
Sbjct: 15  VVKVGTAVVTRSDGRLALGRLGSLCEQLKELNSDGYEVILVTSGAVSAGRQRLRFRKLVN 74

Query: 76  SSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFRRQL 135
           SSFADLQKPQ++LDGKACAAVGQN LMALYDTLFSQLD+T+AQLLVTDNDFRD  FR QL
Sbjct: 75  SSFADLQKPQVELDGKACAAVGQNGLMALYDTLFSQLDLTAAQLLVTDNDFRDPSFRTQL 134

Query: 136 NDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVLLSD 195
            +TV  LL LKV+P+ NENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLL+LLSD
Sbjct: 135 TETVYQLLDLKVVPVLNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLILLSD 194

Query: 196 VAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGIPVV 255
           V GLY+GPPSDP SKLI TY+KE+HQGEITFGDKSR+GRGGMTAKVKAA  AAYAGIPV+
Sbjct: 195 VDGLYNGPPSDPRSKLISTYVKEKHQGEITFGDKSRLGRGGMTAKVKAAVYAAYAGIPVI 254

Query: 256 ITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISSQGR 315
           I SG A +NIIKV+ G  +GTLFH+DAHLW   KE G RDMAVAARESSRRLQA+SS+ R
Sbjct: 255 IASGKATDNIIKVIDGQCVGTLFHKDAHLWVQVKETGVRDMAVAARESSRRLQAVSSEER 314

Query: 316 RKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIRTLA 375
           +KILLDIADALEANE+ I  +NEADV  AQ AGY+++LV+RLA+ P KISSLA SIR LA
Sbjct: 315 KKILLDIADALEANEEKILAENEADVAAAQYAGYDRSLVARLAMNPDKISSLAKSIRVLA 374

Query: 376 NMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLLLKG 435
           +ME+PIG +LKRTE++DGLILEKTS PLGVLLIVFESRPDALVQIASLA+RSGNGLLLKG
Sbjct: 375 DMEEPIGRILKRTEIADGLILEKTSCPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKG 434

Query: 436 GKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVS 495
           GKEA+RSNAILHKVIT+AIPD VG  LIGLVTSR+EIPDLLKLDDVIDLVIPRGSNKLVS
Sbjct: 435 GKEAKRSNAILHKVITSAIPDKVGEKLIGLVTSRDEIPDLLKLDDVIDLVIPRGSNKLVS 494

Query: 496 QIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKDLVP 555
           QIK ST+IPVLGHADGICHVYVDKSA+++MAKRIVLDAK DYPAACNAMETLLVHKDL  
Sbjct: 495 QIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAACNAMETLLVHKDLAE 554

Query: 556 TGGLNELIVNLRIEGVTLYGGPRASSL--LNIPEAQTFHHEYNSLTCTIEIVDDVFAAID 615
            GGLN+LIV+LR EGVT++GGPR  +L   NI   QTF+ EY+S  CT+EIVDDV+AAI+
Sbjct: 555 NGGLNDLIVDLRTEGVTMFGGPRIDALQEFNIQATQTFNREYSSPACTVEIVDDVYAAIE 614

Query: 616 HIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARFG 675
           HI  HG             AHTDCI+ EDH+VA+ FL+ VDSAAV HNASTRFCDG RFG
Sbjct: 615 HINHHG------------SAHTDCIIAEDHKVAETFLQLVDSAAVLHNASTRFCDGFRFG 674

Query: 676 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKI 726
           LGAEVGISTSRIHARGPVGVEGLLTTRW+L+G+GQVV GDKGVVYTHKD+ +
Sbjct: 675 LGAEVGISTSRIHARGPVGVEGLLTTRWVLKGSGQVVHGDKGVVYTHKDLPL 714

BLAST of MS003439 vs. ExPASy Swiss-Prot
Match: P54888 (Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana OX=3702 GN=P5CSB PE=2 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 552/713 (77.42%), Postives = 635/713 (89.06%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGA+CEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ++LDGKACA VGQ+SLMA Y+T+F QLDVT AQ+LVTD+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLVTDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QL++TVK++L ++VIP+FNENDA+STR+APY+DS+GIFWDNDSLAALL+LELKADLL+L
Sbjct: 135 KQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALLSLELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSD  SKLIHT+IKE+HQ EITFG+KS++GRGGMTAKVKAA  AAY G+
Sbjct: 195 LSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKVKAAVNAAYGGV 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGYA ENI KVL+G R+GTLFHQDAHLWAP  +  +RDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R++IL DIA+ALE NEK I  +N+ DV  AQ+AGYE++LV+RL +KPGKISSLA S+R
Sbjct: 315 EDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA MEDPIG VLK+T+V+D LILEKTSSP+GVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 QLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKS  L+MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L   G L++LI  L+ +GVTLYGGPRAS+ LNIPE ++FHHEY+S  CT+EIV+DV+ AI
Sbjct: 555 LEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED EVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKI 726
           GLGAEVGISTSRIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTHKD+ +
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHKDLPV 715

BLAST of MS003439 vs. ExPASy TrEMBL
Match: A0A6J1BY65 (Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC111006663 PE=3 SV=1)

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 698/715 (97.62%), Postives = 700/715 (97.90%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR
Sbjct: 315 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI
Sbjct: 555 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHIYQHG             AHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF
Sbjct: 615 DHIYQHG------------SAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 717

BLAST of MS003439 vs. ExPASy TrEMBL
Match: A0A0A0KBH3 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucumis sativus OX=3659 GN=Csa_6G008780 PE=3 SV=1)

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 658/715 (92.03%), Postives = 687/715 (96.08%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLL+LKV+PIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLTLKVVPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAP NI+KVLKGDRIGTLFHQDAHLW PQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPGNILKVLKGDRIGTLFHQDAHLWTPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIST+NEADV EAQQ+GYEKALVSRLA+KPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISTENEADVAEAQQSGYEKALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+++GG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITEAIPESIGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEM+KRIVLDAKIDYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMSKRIVLDAKIDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TGGLNEL+VNLRIEGVTL GGPRASSLLNIPEA+TFHHEYNSL CT+EIVDDVF AI
Sbjct: 555 LVQTGGLNELVVNLRIEGVTLNGGPRASSLLNIPEARTFHHEYNSLACTLEIVDDVFDAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTEDHEVA+VFLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDHEVAEVFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVVDGDKGVVYTH+DIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVDGDKGVVYTHRDIKIES 717

BLAST of MS003439 vs. ExPASy TrEMBL
Match: A0A1S3B2W5 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucumis melo OX=3656 GN=LOC103485529 PE=3 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 655/715 (91.61%), Postives = 683/715 (95.52%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  +IKVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVIKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLL+LKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLALKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGY P NI+KVLKGDRIGTLFHQDAHLWAPQK+ GARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYTPGNILKVLKGDRIGTLFHQDAHLWAPQKDDGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIST+NEADV EAQQ+GYEKALVSRLA+KPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISTENEADVAEAQQSGYEKALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLKRTEVSDGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKRTEVSDGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+++GG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITGAIPESIGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAK+DYPAACNAMETLLVH D
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKVDYPAACNAMETLLVHND 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TGGLNEL+VNLRIEGVTL GGPRASSLLNIPEA+TFHHEYNSL CT+EIVDDV  AI
Sbjct: 555 LVQTGGLNELVVNLRIEGVTLNGGPRASSLLNIPEARTFHHEYNSLACTLEIVDDVLDAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED EVA+VFLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDREVAEVFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVVDGDKGVVYTH+DIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVDGDKGVVYTHRDIKIES 717

BLAST of MS003439 vs. ExPASy TrEMBL
Match: A0A6J1GSB0 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111457038 PE=3 SV=1)

HSP 1 Score: 1270.8 bits (3287), Expect = 0.0e+00
Identity = 650/715 (90.91%), Postives = 685/715 (95.80%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVTR+DGRLALGRLGALCEQIKELNSQ YE+ILVSSGAVGIGRQRLRYRK
Sbjct: 15  KRLVVKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEYEVILVSSGAVGIGRQRLRYRK 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDV+SAQLLVTDNDFRDKDFR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVSSAQLLVTDNDFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           RQL+DTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 RQLSDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAA GAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAAVGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PVVITSGYAPENI+KVLKGDRIGTLFHQDAHLWAPQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVVITSGYAPENILKVLKGDRIGTLFHQDAHLWAPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + RRKILLD+ADALE NEKLI T+NEADV EAQQ GYE+ALVSRLA+KPGKISSLANSIR
Sbjct: 315 EERRKILLDVADALETNEKLIRTENEADVAEAQQNGYERALVSRLAMKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LANMEDPIG VLK+TE+SDGLILEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 MLANMEDPIGRVLKKTEISDGLILEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHK+ITAAIPD+VGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKIITAAIPDSVGGTLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKVDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TG LNEL+VNLRIEGVTLYGGP+ASSLLNIPEA+TFHHEYNSLTCTIEIV+DVFAAI
Sbjct: 555 LVHTGMLNELVVNLRIEGVTLYGGPKASSLLNIPEARTFHHEYNSLTCTIEIVEDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED +VA++FLRQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDEKVAEIFLRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQVV+GDKGV+YTHKDIKIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQVVNGDKGVIYTHKDIKIES 717

BLAST of MS003439 vs. ExPASy TrEMBL
Match: A0A6J1JTQ8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111489663 PE=3 SV=1)

HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 650/715 (90.91%), Postives = 680/715 (95.10%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVTR+DGRLALGRLGALCEQIKELNSQ +E+ILVSSGAVGIGRQRLRYR+
Sbjct: 15  KRLVVKVGTAVVTRSDGRLALGRLGALCEQIKELNSQEFEVILVSSGAVGIGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ+DLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDK FR
Sbjct: 75  LVNSSFADLQKPQVDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKGFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
            QL+DTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL
Sbjct: 135 SQLSDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLYSGPPSDPHSKLIHTY+KE+HQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI
Sbjct: 195 LSDVDGLYSGPPSDPHSKLIHTYVKERHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGYAP NI+KVLKGDRIGTLFHQDAHLW PQK+VGARDMAVAARESSRRLQAISS
Sbjct: 255 PVIITSGYAPANILKVLKGDRIGTLFHQDAHLWEPQKDVGARDMAVAARESSRRLQAISS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           Q RRKILLDIADALEANEKLIS +NEADV EAQQ GYEKALVSRLALKPGKISSLANSIR
Sbjct: 315 QERRKILLDIADALEANEKLISAENEADVAEAQQTGYEKALVSRLALKPGKISSLANSIR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
           TLANMEDPIGHVLK+TEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 TLANMEDPIGHVLKKTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIPD+VGG +IGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITEAIPDSVGGTIIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVH+D
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHQD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           LV TGGLNEL+VNLRIEGV L GGPR+SSLLNIPEA TFHHEYNSL CT+EIVDDVFAAI
Sbjct: 555 LVHTGGLNELVVNLRIEGVILNGGPRSSSLLNIPEAGTFHHEYNSLACTLEIVDDVFAAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG              HTDCIVTEDHEVA+VF+RQVDSAAVFHNASTRF DGARF
Sbjct: 615 DHIHQHG------------SGHTDCIVTEDHEVAEVFIRQVDSAAVFHNASTRFSDGARF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVGISTSRIHARGPVGVEGLLTTRWILRG+GQ VDGDKGVVYTH+D+KIES
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQTVDGDKGVVYTHRDVKIES 717

BLAST of MS003439 vs. TAIR 10
Match: AT2G39800.1 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 559/715 (78.18%), Postives = 636/715 (88.95%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGALCEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ +LDGKACA VGQ+SLMA Y+T+F QLDVT+AQLLV D+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QLN+TVKS+L L+VIPIFNENDA+STR+APY+DSSGIFWDNDSLAALLALELKADLL+L
Sbjct: 135 KQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSDP+SKLIHT++KE+HQ EITFGDKSR+GRGGMTAKVKAA  AAYAGI
Sbjct: 195 LSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGY+ ENI KVL+G R+GTLFHQDA LWAP  +  ARDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R+KILLDIADALEAN   I  +NE DV  AQ+AG E+++V+RL + PGKISSLA S+R
Sbjct: 315 EDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA+MEDPIG VLK+TEV+DGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 KLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LV+QIK++TKIPVLGHADGICHVYVDK+ D +MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L     LNELI  L+  GVTLYGGPRAS +LNIPEA++F+HEY +  CT+E+V+DV+ AI
Sbjct: 555 LEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI++HG             AHTDCIVTEDHEVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHRHG------------SAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVG+ST RIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTH+DI I++
Sbjct: 675 GLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA 717

BLAST of MS003439 vs. TAIR 10
Match: AT2G39800.4 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 559/715 (78.18%), Postives = 636/715 (88.95%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGALCEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ +LDGKACA VGQ+SLMA Y+T+F QLDVT+AQLLV D+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QLN+TVKS+L L+VIPIFNENDA+STR+APY+DSSGIFWDNDSLAALLALELKADLL+L
Sbjct: 135 KQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSDP+SKLIHT++KE+HQ EITFGDKSR+GRGGMTAKVKAA  AAYAGI
Sbjct: 195 LSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGY+ ENI KVL+G R+GTLFHQDA LWAP  +  ARDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R+KILLDIADALEAN   I  +NE DV  AQ+AG E+++V+RL + PGKISSLA S+R
Sbjct: 315 EDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA+MEDPIG VLK+TEV+DGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 KLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LV+QIK++TKIPVLGHADGICHVYVDK+ D +MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L     LNELI  L+  GVTLYGGPRAS +LNIPEA++F+HEY +  CT+E+V+DV+ AI
Sbjct: 555 LEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI++HG             AHTDCIVTEDHEVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHRHG------------SAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVG+ST RIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTH+DI I++
Sbjct: 675 GLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA 717

BLAST of MS003439 vs. TAIR 10
Match: AT3G55610.1 (delta 1-pyrroline-5-carboxylate synthase 2 )

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 552/713 (77.42%), Postives = 635/713 (89.06%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGA+CEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ++LDGKACA VGQ+SLMA Y+T+F QLDVT AQ+LVTD+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLVTDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QL++TVK++L ++VIP+FNENDA+STR+APY+DS+GIFWDNDSLAALL+LELKADLL+L
Sbjct: 135 KQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALLSLELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSD  SKLIHT+IKE+HQ EITFG+KS++GRGGMTAKVKAA  AAY G+
Sbjct: 195 LSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKVKAAVNAAYGGV 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGYA ENI KVL+G R+GTLFHQDAHLWAP  +  +RDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R++IL DIA+ALE NEK I  +N+ DV  AQ+AGYE++LV+RL +KPGKISSLA S+R
Sbjct: 315 EDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA MEDPIG VLK+T+V+D LILEKTSSP+GVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 QLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LVSQIK+STKIPVLGHADGICHVYVDKS  L+MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L   G L++LI  L+ +GVTLYGGPRAS+ LNIPE ++FHHEY+S  CT+EIV+DV+ AI
Sbjct: 555 LEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI+QHG             AHTDCIVTED EVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHQHG------------SAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKI 726
           GLGAEVGISTSRIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTHKD+ +
Sbjct: 675 GLGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHKDLPV 715

BLAST of MS003439 vs. TAIR 10
Match: AT2G39800.3 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 556/715 (77.76%), Postives = 633/715 (88.53%), Query Frame = 0

Query: 13  RAFIIKVGTAVVTRTDGRLALGRLGALCEQIKELNSQGYEIILVSSGAVGIGRQRLRYRK 72
           +  ++KVGTAVVT   GRLALGRLGALCEQ+ ELNS G+E+ILVSSGAVG+GRQRLRYR+
Sbjct: 15  KRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQ 74

Query: 73  LVNSSFADLQKPQIDLDGKACAAVGQNSLMALYDTLFSQLDVTSAQLLVTDNDFRDKDFR 132
           LVNSSFADLQKPQ +LDGKACA VGQ+SLMA Y+T+F QLDVT+AQLLV D+ FRDKDFR
Sbjct: 75  LVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFR 134

Query: 133 RQLNDTVKSLLSLKVIPIFNENDAVSTRKAPYEDSSGIFWDNDSLAALLALELKADLLVL 192
           +QLN+TVKS+L L+VIPIFNENDA+STR+APY+DSSGIFWDNDSLAALLALELKADLL+L
Sbjct: 135 KQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALLALELKADLLIL 194

Query: 193 LSDVAGLYSGPPSDPHSKLIHTYIKEQHQGEITFGDKSRVGRGGMTAKVKAATGAAYAGI 252
           LSDV GLY+GPPSDP+SKLIHT++KE+HQ EITFGDKSR+GRGGMTAKVKAA  AAYAGI
Sbjct: 195 LSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGI 254

Query: 253 PVVITSGYAPENIIKVLKGDRIGTLFHQDAHLWAPQKEVGARDMAVAARESSRRLQAISS 312
           PV+ITSGY+ ENI KVL+G R+GTLFHQDA LWAP  +  ARDMAVAARESSR+LQA+SS
Sbjct: 255 PVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSS 314

Query: 313 QGRRKILLDIADALEANEKLISTQNEADVTEAQQAGYEKALVSRLALKPGKISSLANSIR 372
           + R+KILLDIADALEAN   I  +NE DV  AQ+AG E+++V+RL + PGKISSLA S+R
Sbjct: 315 EDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVR 374

Query: 373 TLANMEDPIGHVLKRTEVSDGLILEKTSSPLGVLLIVFESRPDALVQIASLAVRSGNGLL 432
            LA+MEDPIG VLK+TEV+DGL+LEKTSSPLGVLLIVFESRPDALVQIASLA+RSGNGLL
Sbjct: 375 KLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLL 434

Query: 433 LKGGKEARRSNAILHKVITAAIPDTVGGGLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 492
           LKGGKEARRSNAILHKVIT AIP+TVGG LIGLVTSREEIPDLLKLDDVIDLVIPRGSNK
Sbjct: 435 LKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNK 494

Query: 493 LVSQIKSSTKIPVLGHADGICHVYVDKSADLEMAKRIVLDAKIDYPAACNAMETLLVHKD 552
           LV+QIK++TKIPVL   DGICHVYVDK+ D +MAKRIV DAK+DYPAACNAMETLLVHKD
Sbjct: 495 LVTQIKNTTKIPVL---DGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKD 554

Query: 553 LVPTGGLNELIVNLRIEGVTLYGGPRASSLLNIPEAQTFHHEYNSLTCTIEIVDDVFAAI 612
           L     LNELI  L+  GVTLYGGPRAS +LNIPEA++F+HEY +  CT+E+V+DV+ AI
Sbjct: 555 LEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAI 614

Query: 613 DHIYQHGYFLMKDDLKLLFFAHTDCIVTEDHEVAKVFLRQVDSAAVFHNASTRFCDGARF 672
           DHI++HG             AHTDCIVTEDHEVA++FLRQVDSAAVFHNASTRF DG RF
Sbjct: 615 DHIHRHG------------SAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRF 674

Query: 673 GLGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVDGDKGVVYTHKDIKIES 728
           GLGAEVG+ST RIHARGPVGVEGLLTTRWI+RG GQVVDGD G+VYTH+DI I++
Sbjct: 675 GLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQA 714

BLAST of MS003439 vs. TAIR 10
Match: AT2G39800.2 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 971.8 bits (2511), Expect = 2.9e-283
Identity = 489/626 (78.12%), Postives = 556/626 (88.82%), Query Frame = 0

Query: 102 MALYDTLFSQLDVTSAQLLVTDNDFRDKDFRRQLNDTVKSLLSLKVIPIFNENDAVSTRK 161
           MA Y+T+F QLDVT+AQLLV D+ FRDKDFR+QLN+TVKS+L L+VIPIFNENDA+STR+
Sbjct: 1   MAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRR 60

Query: 162 APYEDSSGIFWDNDSLAALLALELKADLLVLLSDVAGLYSGPPSDPHSKLIHTYIKEQHQ 221
           APY+DSSGIFWDNDSLAALLALELKADLL+LLSDV GLY+GPPSDP+SKLIHT++KE+HQ
Sbjct: 61  APYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQ 120

Query: 222 GEITFGDKSRVGRGGMTAKVKAATGAAYAGIPVVITSGYAPENIIKVLKGDRIGTLFHQD 281
            EITFGDKSR+GRGGMTAKVKAA  AAYAGIPV+ITSGY+ ENI KVL+G R+GTLFHQD
Sbjct: 121 DEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQD 180

Query: 282 AHLWAPQKEVGARDMAVAARESSRRLQAISSQGRRKILLDIADALEANEKLISTQNEADV 341
           A LWAP  +  ARDMAVAARESSR+LQA+SS+ R+KILLDIADALEAN   I  +NE DV
Sbjct: 181 ARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDV 240

Query: 342 TEAQQAGYEKALVSRLALKPGKISSLANSIRTLANMEDPIGHVLKRTEVSDGLILEKTSS 401
             AQ+AG E+++V+RL + PGKISSLA S+R LA+MEDPIG VLK+TEV+DGL+LEKTSS
Sbjct: 241 ASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSS 300

Query: 402 PLGVLLIVFESRPDALVQIASLAVRSGNGLLLKGGKEARRSNAILHKVITAAIPDTVGGG 461
           PLGVLLIVFESRPDALVQIASLA+RSGNGLLLKGGKEARRSNAILHKVIT AIP+TVGG 
Sbjct: 301 PLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGK 360

Query: 462 LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKSSTKIPVLGHADGICHVYVDKSA 521
           LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK++TKIPVLGHADGICHVYVDK+ 
Sbjct: 361 LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKAC 420

Query: 522 DLEMAKRIVLDAKIDYPAACNAMETLLVHKDLVPTGGLNELIVNLRIEGVTLYGGPRASS 581
           D +MAKRIV DAK+DYPAACNAMETLLVHKDL     LNELI  L+  GVTLYGGPRAS 
Sbjct: 421 DTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASK 480

Query: 582 LLNIPEAQTFHHEYNSLTCTIEIVDDVFAAIDHIYQHGYFLMKDDLKLLFFAHTDCIVTE 641
           +LNIPEA++F+HEY +  CT+E+V+DV+ AIDHI++HG             AHTDCIVTE
Sbjct: 481 ILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHG------------SAHTDCIVTE 540

Query: 642 DHEVAKVFLRQVDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRW 701
           DHEVA++FLRQVDSAAVFHNASTRF DG RFGLGAEVG+ST RIHARGPVGVEGLLTTRW
Sbjct: 541 DHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRW 600

Query: 702 ILRGTGQVVDGDKGVVYTHKDIKIES 728
           I+RG GQVVDGD G+VYTH+DI I++
Sbjct: 601 IMRGKGQVVDGDNGIVYTHQDIPIQA 614