MS003423 (gene) Bitter gourd (TR) v1

Overview
NameMS003423
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein of unknown function (DUF620)
Locationscaffold234: 2779816 .. 2782143 (+)
RNA-Seq ExpressionMS003423
SyntenyMS003423
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGCTTTGTCCGAATTTCGACCGAGAAGACGGTCTCGACACTGTCCTTGAGGTTCCCATTCCTGAAGAGATGTTCTCTTGCAACACTAACAAAACCCACGCGATTTCATGGCAAGCTATGAAATCGTGGGTGAAGTCCCATAATGATCATAAATCGCACGTGAATTCCATGGCTGCACTTTTCGGCGGCCGGAACGCCGAGATCCAGCTCCTCCTCGGCGTCGTCGGAGCTCCTTTAATCCCTCTCCCTGTCCCTTTCGATCACCGACCCATTACCCGCAACATCAAAGACAACCCCATTGTAAAAAGATCTCAACTTTAATCGTTTTTCCTTTTTTTTTTCTCTTTTAAAATTCGTGAGAATTAATTATGGGGTTTTTTTTTTTAATAGGAGGCGTCGATGGCGAAGTATATAGTGCAGCAATATGTGGCGGCGGTGGGAGGGGAGCATGCGTTGAACTCGATTGAGAGTATGTATGCGATGGGGAAGGTGAAGATGGTGGCGTCGGAATTCTCGTCCGGCGAGGGGAGTTTGAACGGAAAGGTGTTAAAGGCGAAGAACGGGAAAGGCGGCGGCGGCGGAGAGATGGGTGGGTTTGTGGTGTGGCAGAAACGGCCGGAGTTATGGTGCTTGGAACTGATGCTGTCCGGTTGTAAAATCAGCGCCGGCAGCGATGGGAAAGTGGCTTGGAGACAAACTCCATGGCATCATTCTCATGCTTCTCGTGGCCCTCCTCGTCCCCTCCGACGCTTCTTGCAGGTAACTCGCCGGAATTTTTACACCCTTAACAACAAAATTTCAGGATTTTTCATAATTTCGAGATAAATTACTAGCTATCTTTTAGTTCTTGTTAATTAATAAATCATGTTATATTAGTAATATTTTAAGTGTTTACATGAATAAAATAAAACAACAAGCTGTTAATTAATTTTTTAACTGACCCACCCTAAACTGTTTGATAATTATGATATAAATGAAAATCAATTTTTTTTTTATAATGAGTTATCAAATTTTCCACAGTGTAAGGACAATATTAACTAAAAATACACTTATGTTATATTGGATTTTGAAAAATATATATTAAATTTATGTTTGAATAATATCTTTTGTATTCCAAGTTTAGAAAAATAAAATAAGATAATTTTTGTAATCAAACCAGGGTCAATTCATTGAGTTTCAAAATTCCAACATTTGTGTGTTTTGGGCGCTGATTTTATTATTATTATCATGGTTTTGAAATTGTAGTGTGAATGATCTGTGCCCAAGATTTTCAAGGTGTGAAATAATGATTCGATTGAGAGGATGAGGATAAAATCAACAGAATTTTCAATGCACCTAATCTTATTCTAAAGTTATTAGAATAATAGTGCATTTGGTGGGAACCACCTGAACGTGGAGGCTACCATGGTTGAAACATAATTCATAAACCACTTTTTCTTGCTCTTCGGCCATATAAAATTTTCCATTTTCTGAAATATATTTTGAAAAGTCCATTTCAAGTAAAGTTGTTAAGACAGCGGTGAATCCCAAATTCGATGAGAAATTGGAGTAAGCCCAAAGATATATTTGGGCCCAAATGACCCAAATATATCAAATGGGCTTTCCAGGTCCAGGGCCCACTTATGACTTAAATAGTTTCGATGAAAATTACTAAAATACCTGGTGATTTTTTGACAGGGGCTTGATCCGAAATCGACGGCGACTCTGTTTTCGAATTCCAGCTGCATCGGCGAGAAAACAATCAACGATGAAGATTGTTTCATCCTAAAACTGGAAGCGGAATCAACGGTGCTGAGAGCGAGAAGCAGTAGTAGCGTGGAGATCATCCGCCACACGGTTTGGGGATACTTCAGCCAGAGAACCGGCCTCCTGGTCCAGCTCGAAGACTCGCATCTCCTCCGAATCAAGGCCGGTGGATCCCGAAACGACAGTATTTTCTGGGAAACGACAATGGAAACCCTAATTCAAGACTACAGAACGATCGACGGCGTCAATATCGCACACGCCGGGAAAACGTCCGTTTCGCTCTTTCGATTCGGCGAGAGCGCTGAAGGCCATTCGAGAACGAAAATGGAAGAGATTTGGGAGATCGAAGAAGTGGATTTCAATATCAAGGGTTTGTCGATGGACTTCTTTTTGCCTCCTAGCGATCTGAAGAAGGAGGAAGAAGGAGTCGCCGTGATTACGAGTAACGGAAAGTTTCCGTTGACGATTCGATGTACGGCTGCTGCTTCGAAGATTTGTTCGTCGCGAGTGGCGGCCATTGATGTTGATGAAATTTCGGAGGGGAGCAGCAACCAGAGTGATGAGGATGAAGATGAA

mRNA sequence

ATGAGGAAGCTTTGTCCGAATTTCGACCGAGAAGACGGTCTCGACACTGTCCTTGAGGTTCCCATTCCTGAAGAGATGTTCTCTTGCAACACTAACAAAACCCACGCGATTTCATGGCAAGCTATGAAATCGTGGGTGAAGTCCCATAATGATCATAAATCGCACGTGAATTCCATGGCTGCACTTTTCGGCGGCCGGAACGCCGAGATCCAGCTCCTCCTCGGCGTCGTCGGAGCTCCTTTAATCCCTCTCCCTGTCCCTTTCGATCACCGACCCATTACCCGCAACATCAAAGACAACCCCATTGAGGCGTCGATGGCGAAGTATATAGTGCAGCAATATGTGGCGGCGGTGGGAGGGGAGCATGCGTTGAACTCGATTGAGAGTATGTATGCGATGGGGAAGGTGAAGATGGTGGCGTCGGAATTCTCGTCCGGCGAGGGGAGTTTGAACGGAAAGGTGTTAAAGGCGAAGAACGGGAAAGGCGGCGGCGGCGGAGAGATGGGTGGGTTTGTGGTGTGGCAGAAACGGCCGGAGTTATGGTGCTTGGAACTGATGCTGTCCGGTTGTAAAATCAGCGCCGGCAGCGATGGGAAAGTGGCTTGGAGACAAACTCCATGGCATCATTCTCATGCTTCTCGTGGCCCTCCTCGTCCCCTCCGACGCTTCTTGCAGGGGCTTGATCCGAAATCGACGGCGACTCTGTTTTCGAATTCCAGCTGCATCGGCGAGAAAACAATCAACGATGAAGATTGTTTCATCCTAAAACTGGAAGCGGAATCAACGGTGCTGAGAGCGAGAAGCAGTAGTAGCGTGGAGATCATCCGCCACACGGTTTGGGGATACTTCAGCCAGAGAACCGGCCTCCTGGTCCAGCTCGAAGACTCGCATCTCCTCCGAATCAAGGCCGGTGGATCCCGAAACGACAGTATTTTCTGGGAAACGACAATGGAAACCCTAATTCAAGACTACAGAACGATCGACGGCGTCAATATCGCACACGCCGGGAAAACGTCCGTTTCGCTCTTTCGATTCGGCGAGAGCGCTGAAGGCCATTCGAGAACGAAAATGGAAGAGATTTGGGAGATCGAAGAAGTGGATTTCAATATCAAGGGTTTGTCGATGGACTTCTTTTTGCCTCCTAGCGATCTGAAGAAGGAGGAAGAAGGAGTCGCCGTGATTACGAGTAACGGAAAGTTTCCGTTGACGATTCGATGTACGGCTGCTGCTTCGAAGATTTGTTCGTCGCGAGTGGCGGCCATTGATGTTGATGAAATTTCGGAGGGGAGCAGCAACCAGAGTGATGAGGATGAAGATGAA

Coding sequence (CDS)

ATGAGGAAGCTTTGTCCGAATTTCGACCGAGAAGACGGTCTCGACACTGTCCTTGAGGTTCCCATTCCTGAAGAGATGTTCTCTTGCAACACTAACAAAACCCACGCGATTTCATGGCAAGCTATGAAATCGTGGGTGAAGTCCCATAATGATCATAAATCGCACGTGAATTCCATGGCTGCACTTTTCGGCGGCCGGAACGCCGAGATCCAGCTCCTCCTCGGCGTCGTCGGAGCTCCTTTAATCCCTCTCCCTGTCCCTTTCGATCACCGACCCATTACCCGCAACATCAAAGACAACCCCATTGAGGCGTCGATGGCGAAGTATATAGTGCAGCAATATGTGGCGGCGGTGGGAGGGGAGCATGCGTTGAACTCGATTGAGAGTATGTATGCGATGGGGAAGGTGAAGATGGTGGCGTCGGAATTCTCGTCCGGCGAGGGGAGTTTGAACGGAAAGGTGTTAAAGGCGAAGAACGGGAAAGGCGGCGGCGGCGGAGAGATGGGTGGGTTTGTGGTGTGGCAGAAACGGCCGGAGTTATGGTGCTTGGAACTGATGCTGTCCGGTTGTAAAATCAGCGCCGGCAGCGATGGGAAAGTGGCTTGGAGACAAACTCCATGGCATCATTCTCATGCTTCTCGTGGCCCTCCTCGTCCCCTCCGACGCTTCTTGCAGGGGCTTGATCCGAAATCGACGGCGACTCTGTTTTCGAATTCCAGCTGCATCGGCGAGAAAACAATCAACGATGAAGATTGTTTCATCCTAAAACTGGAAGCGGAATCAACGGTGCTGAGAGCGAGAAGCAGTAGTAGCGTGGAGATCATCCGCCACACGGTTTGGGGATACTTCAGCCAGAGAACCGGCCTCCTGGTCCAGCTCGAAGACTCGCATCTCCTCCGAATCAAGGCCGGTGGATCCCGAAACGACAGTATTTTCTGGGAAACGACAATGGAAACCCTAATTCAAGACTACAGAACGATCGACGGCGTCAATATCGCACACGCCGGGAAAACGTCCGTTTCGCTCTTTCGATTCGGCGAGAGCGCTGAAGGCCATTCGAGAACGAAAATGGAAGAGATTTGGGAGATCGAAGAAGTGGATTTCAATATCAAGGGTTTGTCGATGGACTTCTTTTTGCCTCCTAGCGATCTGAAGAAGGAGGAAGAAGGAGTCGCCGTGATTACGAGTAACGGAAAGTTTCCGTTGACGATTCGATGTACGGCTGCTGCTTCGAAGATTTGTTCGTCGCGAGTGGCGGCCATTGATGTTGATGAAATTTCGGAGGGGAGCAGCAACCAGAGTGATGAGGATGAAGATGAA

Protein sequence

MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMAALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGGEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKICSSRVAAIDVDEISEGSSNQSDEDEDE
Homology
BLAST of MS003423 vs. NCBI nr
Match: XP_022133661.1 (uncharacterized protein LOC111006191 [Momordica charantia])

HSP 1 Score: 880.6 bits (2274), Expect = 5.8e-252
Identity = 437/440 (99.32%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA 60

Query: 61  ALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG 120
           ALFGGRNAEIQLLLGVVGAPLIP+PVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG
Sbjct: 61  ALFGGRNAEIQLLLGVVGAPLIPIPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG 120

Query: 121 EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL 180
           EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL
Sbjct: 121 EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL 180

Query: 181 WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS 240
           WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS
Sbjct: 181 WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS 240

Query: 241 CIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR 300
           C+GEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR
Sbjct: 241 CMGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR 300

Query: 301 IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI 360
           IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI
Sbjct: 301 IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI 360

Query: 361 WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKICSSRVAA 420
           WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLT+RCTAAASKICSSRVAA
Sbjct: 361 WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTMRCTAAASKICSSRVAA 420

Query: 421 IDVDEISEGSSNQSDEDEDE 441
           IDVDEISEGSSNQSDEDEDE
Sbjct: 421 IDVDEISEGSSNQSDEDEDE 440

BLAST of MS003423 vs. NCBI nr
Match: XP_022957502.1 (uncharacterized protein LOC111458877 [Cucurbita moschata])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 403/447 (90.16%), Postives = 418/447 (93.51%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKS-HNDHKSHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNK HAISWQAMK+WVKS H+D  SHVNS+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKAHAISWQAMKTWVKSNHHDKSSHVNSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+PIT NIKDNPIEASMAKYIVQQYVAAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIIFHHQPITCNIKDNPIEASMAKYIVQQYVAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGG----GGGEMGGFVVWQ 180
           GEHALNSI+SMYAMGKVKMVASEFSSGE  LNGK  K KNGKGG    GGGEMGGFVVWQ
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFSSGEACLNGKATKVKNGKGGGGVVGGGEMGGFVVWQ 180

Query: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240
           KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL
Sbjct: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240

Query: 241 FSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLED 300
           FSNS+CIGEKTINDEDCFILKLEAES VLRARSSSSVEIIRHTVWGYFSQRTGLLV+LED
Sbjct: 241 FSNSTCIGEKTINDEDCFILKLEAESPVLRARSSSSVEIIRHTVWGYFSQRTGLLVELED 300

Query: 301 SHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRT 360
           SHLLRIKAGGSRND IFWETTMETLIQDYRTIDGVNIAHAGKT+VSLFRFGESAEGHS+T
Sbjct: 301 SHLLRIKAGGSRNDDIFWETTMETLIQDYRTIDGVNIAHAGKTTVSLFRFGESAEGHSKT 360

Query: 361 KMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAA---SK 420
           KMEE WEIEEVDFNI+GLSMDFFLPPSDLKKE+EGV   TSNGK PLT+RC AAA   SK
Sbjct: 361 KMEETWEIEEVDFNIQGLSMDFFLPPSDLKKEKEGVGA-TSNGKLPLTMRCAAAAAAGSK 420

Query: 421 ICSSRVAAIDVDEISEGSSNQSDEDED 440
           ICSSRVAAIDVDE SEG SNQSDEDE+
Sbjct: 421 ICSSRVAAIDVDE-SEG-SNQSDEDEE 444

BLAST of MS003423 vs. NCBI nr
Match: XP_022990734.1 (uncharacterized protein LOC111487531 [Cucurbita maxima])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 398/440 (90.45%), Postives = 415/440 (94.32%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHK-SHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNK HAISWQAMK+WVKS+  HK SHVNS+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKAHAISWQAMKTWVKSNQHHKSSHVNSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+PIT NIKDNPIEASMAKYIVQQYVAAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIIFHHQPITCNIKDNPIEASMAKYIVQQYVAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPE 180
           GEHALNSI+SMYAMGKVKMVASEFSSGE  LNGK  K KNGKGGGGGEMGGFVVWQKRPE
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFSSGEACLNGKATKVKNGKGGGGGEMGGFVVWQKRPE 180

Query: 181 LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS 240
           LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS
Sbjct: 181 LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS 240

Query: 241 SCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLL 300
           +C+GEKTINDEDCFIL LEAES VLRARSSSSVEIIRHTVWGYFSQRTGLLV+LEDSHLL
Sbjct: 241 TCVGEKTINDEDCFILNLEAESPVLRARSSSSVEIIRHTVWGYFSQRTGLLVELEDSHLL 300

Query: 301 RIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEE 360
           RIKAGGSRND IFWETTMETLIQDYRTIDGVNIAHAGKT+VSLFRFGESAEGHS+TKMEE
Sbjct: 301 RIKAGGSRNDDIFWETTMETLIQDYRTIDGVNIAHAGKTTVSLFRFGESAEGHSKTKMEE 360

Query: 361 IWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKICSSRVA 420
            WEIEEVDFNI+GLSMDFFLPPSDLKKE+EGV   TSNGK PLT+RC A+ SKIC SRVA
Sbjct: 361 TWEIEEVDFNIQGLSMDFFLPPSDLKKEKEGVGA-TSNGKLPLTMRCAASGSKIC-SRVA 420

Query: 421 AIDVDEISEGSSNQSDEDED 440
           AIDVDE SEG SNQSDEDE+
Sbjct: 421 AIDVDE-SEG-SNQSDEDEE 436

BLAST of MS003423 vs. NCBI nr
Match: KAG6602636.1 (hypothetical protein SDJN03_07869, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033321.1 hypothetical protein SDJN02_07376 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 784.3 bits (2024), Expect = 5.6e-223
Identity = 400/448 (89.29%), Postives = 416/448 (92.86%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVK-SHNDHKSHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNK HAISWQAMK+WVK +H+D  SH NS+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKAHAISWQAMKTWVKPNHHDKSSHANSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+PIT NIKDNPIEASMAKYIVQQYVAAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIIFHHQPITCNIKDNPIEASMAKYIVQQYVAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGG----GGGEMGGFVVWQ 180
           GEHALNSI+SMYAMGKVKMVASEFSSGE  LNGK  K KNGKGG    GGGEMGGFVVWQ
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFSSGEACLNGKATKVKNGKGGGGVVGGGEMGGFVVWQ 180

Query: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240
           KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL
Sbjct: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240

Query: 241 FSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLED 300
           FSNS+CIGEKTINDEDCFILKLEAES VLRARSSSSVEIIRHTVWGYFSQRTGLLV+LED
Sbjct: 241 FSNSTCIGEKTINDEDCFILKLEAESPVLRARSSSSVEIIRHTVWGYFSQRTGLLVELED 300

Query: 301 SHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRT 360
           SHLLRIKAGGSRND IFWETTMETLIQDYRTID VNIAHAGKT+VSLFRFGESAEGHS+T
Sbjct: 301 SHLLRIKAGGSRNDDIFWETTMETLIQDYRTIDAVNIAHAGKTTVSLFRFGESAEGHSKT 360

Query: 361 KMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAA----S 420
           KMEE WEIEEVDFNI+GLSMDFFLPPSDLKKE+EGV   TSNGK PLT+RC AAA    S
Sbjct: 361 KMEETWEIEEVDFNIQGLSMDFFLPPSDLKKEKEGVGA-TSNGKLPLTMRCAAAAAAAGS 420

Query: 421 KICSSRVAAIDVDEISEGSSNQSDEDED 440
           KICSSRVAAIDVDE SEG SNQSDEDE+
Sbjct: 421 KICSSRVAAIDVDE-SEG-SNQSDEDEE 445

BLAST of MS003423 vs. NCBI nr
Match: XP_038886072.1 (uncharacterized protein LOC120076338 [Benincasa hispida])

HSP 1 Score: 765.0 bits (1974), Expect = 3.5e-217
Identity = 388/446 (87.00%), Postives = 409/446 (91.70%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKS--HNDHKSHVNS 60
           MRKLCPNFDRE GLDTVLEVPIPEEMFS  T KTH ISWQAMKSWVKS  H+D  SHV S
Sbjct: 1   MRKLCPNFDREYGLDTVLEVPIPEEMFSSTTTKTHTISWQAMKSWVKSNHHHDKSSHVGS 60

Query: 61  MAALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAV 120
           +++LFGGRNAEIQLLLGVVGAPLIPLP+ FD +PITRNIKDNPIEASMAKYIVQQY+AAV
Sbjct: 61  ISSLFGGRNAEIQLLLGVVGAPLIPLPITFDQQPITRNIKDNPIEASMAKYIVQQYIAAV 120

Query: 121 GGEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKA--KNGK--GGGGGEMGGFVVW 180
           GGEHALNSI+SMYAMGKVKM ASEF SGEG LNGK +K    NGK  GGGGGEMGGFVVW
Sbjct: 121 GGEHALNSIDSMYAMGKVKMAASEFCSGEGCLNGKAVKGGKNNGKGGGGGGGEMGGFVVW 180

Query: 181 QKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTAT 240
           QKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTAT
Sbjct: 181 QKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTAT 240

Query: 241 LFSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLE 300
           LFSNS+CIGEKTINDEDCFILKLEAES+VLRARSSSSVEIIRHTVWGYFSQRTGLLVQLE
Sbjct: 241 LFSNSTCIGEKTINDEDCFILKLEAESSVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLE 300

Query: 301 DSHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSR 360
           DSHLLRIK  GSRND+IFWETTMETLIQDYRTIDGVNIAHAGKT+VSLFRFGE+AEGHS+
Sbjct: 301 DSHLLRIKPAGSRNDNIFWETTMETLIQDYRTIDGVNIAHAGKTTVSLFRFGETAEGHSK 360

Query: 361 TKMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKIC 420
           TKMEE WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGV +ITSNGKFP+T+RC +A S++ 
Sbjct: 361 TKMEENWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVGMITSNGKFPVTMRC-SAGSRMF 420

Query: 421 SSRVAAID-VDEISEGSSNQSDEDED 440
           SSRV AID  DE     SNQSDEDED
Sbjct: 421 SSRVVAIDQSDEDESEESNQSDEDED 445

BLAST of MS003423 vs. ExPASy TrEMBL
Match: A0A6J1BVR5 (uncharacterized protein LOC111006191 OS=Momordica charantia OX=3673 GN=LOC111006191 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 2.8e-252
Identity = 437/440 (99.32%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA 60

Query: 61  ALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG 120
           ALFGGRNAEIQLLLGVVGAPLIP+PVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG
Sbjct: 61  ALFGGRNAEIQLLLGVVGAPLIPIPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG 120

Query: 121 EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL 180
           EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL
Sbjct: 121 EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL 180

Query: 181 WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS 240
           WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS
Sbjct: 181 WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS 240

Query: 241 CIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR 300
           C+GEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR
Sbjct: 241 CMGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR 300

Query: 301 IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI 360
           IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI
Sbjct: 301 IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI 360

Query: 361 WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKICSSRVAA 420
           WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLT+RCTAAASKICSSRVAA
Sbjct: 361 WEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTMRCTAAASKICSSRVAA 420

Query: 421 IDVDEISEGSSNQSDEDEDE 441
           IDVDEISEGSSNQSDEDEDE
Sbjct: 421 IDVDEISEGSSNQSDEDEDE 440

BLAST of MS003423 vs. ExPASy TrEMBL
Match: A0A6J1JSU8 (uncharacterized protein LOC111487531 OS=Cucurbita maxima OX=3661 GN=LOC111487531 PE=4 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.5e-225
Identity = 398/440 (90.45%), Postives = 415/440 (94.32%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHK-SHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNK HAISWQAMK+WVKS+  HK SHVNS+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKAHAISWQAMKTWVKSNQHHKSSHVNSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+PIT NIKDNPIEASMAKYIVQQYVAAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIIFHHQPITCNIKDNPIEASMAKYIVQQYVAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPE 180
           GEHALNSI+SMYAMGKVKMVASEFSSGE  LNGK  K KNGKGGGGGEMGGFVVWQKRPE
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFSSGEACLNGKATKVKNGKGGGGGEMGGFVVWQKRPE 180

Query: 181 LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS 240
           LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS
Sbjct: 181 LWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNS 240

Query: 241 SCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLL 300
           +C+GEKTINDEDCFIL LEAES VLRARSSSSVEIIRHTVWGYFSQRTGLLV+LEDSHLL
Sbjct: 241 TCVGEKTINDEDCFILNLEAESPVLRARSSSSVEIIRHTVWGYFSQRTGLLVELEDSHLL 300

Query: 301 RIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEE 360
           RIKAGGSRND IFWETTMETLIQDYRTIDGVNIAHAGKT+VSLFRFGESAEGHS+TKMEE
Sbjct: 301 RIKAGGSRNDDIFWETTMETLIQDYRTIDGVNIAHAGKTTVSLFRFGESAEGHSKTKMEE 360

Query: 361 IWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAASKICSSRVA 420
            WEIEEVDFNI+GLSMDFFLPPSDLKKE+EGV   TSNGK PLT+RC A+ SKIC SRVA
Sbjct: 361 TWEIEEVDFNIQGLSMDFFLPPSDLKKEKEGVGA-TSNGKLPLTMRCAASGSKIC-SRVA 420

Query: 421 AIDVDEISEGSSNQSDEDED 440
           AIDVDE SEG SNQSDEDE+
Sbjct: 421 AIDVDE-SEG-SNQSDEDEE 436

BLAST of MS003423 vs. ExPASy TrEMBL
Match: A0A6J1GZA9 (uncharacterized protein LOC111458877 OS=Cucurbita moschata OX=3662 GN=LOC111458877 PE=4 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.5e-225
Identity = 403/447 (90.16%), Postives = 418/447 (93.51%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKS-HNDHKSHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNK HAISWQAMK+WVKS H+D  SHVNS+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKAHAISWQAMKTWVKSNHHDKSSHVNSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+PIT NIKDNPIEASMAKYIVQQYVAAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIIFHHQPITCNIKDNPIEASMAKYIVQQYVAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGG----GGGEMGGFVVWQ 180
           GEHALNSI+SMYAMGKVKMVASEFSSGE  LNGK  K KNGKGG    GGGEMGGFVVWQ
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFSSGEACLNGKATKVKNGKGGGGVVGGGEMGGFVVWQ 180

Query: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240
           KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL
Sbjct: 181 KRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATL 240

Query: 241 FSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLED 300
           FSNS+CIGEKTINDEDCFILKLEAES VLRARSSSSVEIIRHTVWGYFSQRTGLLV+LED
Sbjct: 241 FSNSTCIGEKTINDEDCFILKLEAESPVLRARSSSSVEIIRHTVWGYFSQRTGLLVELED 300

Query: 301 SHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRT 360
           SHLLRIKAGGSRND IFWETTMETLIQDYRTIDGVNIAHAGKT+VSLFRFGESAEGHS+T
Sbjct: 301 SHLLRIKAGGSRNDDIFWETTMETLIQDYRTIDGVNIAHAGKTTVSLFRFGESAEGHSKT 360

Query: 361 KMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIRCTAAA---SK 420
           KMEE WEIEEVDFNI+GLSMDFFLPPSDLKKE+EGV   TSNGK PLT+RC AAA   SK
Sbjct: 361 KMEETWEIEEVDFNIQGLSMDFFLPPSDLKKEKEGVGA-TSNGKLPLTMRCAAAAAAGSK 420

Query: 421 ICSSRVAAIDVDEISEGSSNQSDEDED 440
           ICSSRVAAIDVDE SEG SNQSDEDE+
Sbjct: 421 ICSSRVAAIDVDE-SEG-SNQSDEDEE 444

BLAST of MS003423 vs. ExPASy TrEMBL
Match: A0A6J1FGT4 (uncharacterized protein LOC111445268 OS=Cucurbita moschata OX=3662 GN=LOC111445268 PE=4 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 7.2e-208
Identity = 370/444 (83.33%), Postives = 403/444 (90.77%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHK-SHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSC T K+HAISWQAMKSWVKS   +K SH+ S+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCPTYKSHAISWQAMKSWVKSSYYNKPSHLTSI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+ ITRNIKDNPIEASMAKYIVQQY+AAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIAFHHQSITRNIKDNPIEASMAKYIVQQYIAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGG-GGGEMGGFVVWQKRP 180
           GEHALNSI+SMYAMGKVKMVASEF+SGEG  NGK +KAKNGKGG   GEMG FV+WQKRP
Sbjct: 121 GEHALNSIDSMYAMGKVKMVASEFTSGEGCFNGKPMKAKNGKGGISSGEMGSFVIWQKRP 180

Query: 181 ELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN 240
           +LWCLE+MLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN
Sbjct: 181 DLWCLEMMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN 240

Query: 241 SSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHL 300
           S+CIGEKT+N EDCFILKLEAES+VLRARSSS VEIIRHTVWGYFSQRTGLLV LEDSHL
Sbjct: 241 STCIGEKTMNGEDCFILKLEAESSVLRARSSSGVEIIRHTVWGYFSQRTGLLVHLEDSHL 300

Query: 301 LRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKME 360
           LRIK GGSRND++FWETTME+ IQDYRTIDGVNIAHAGKT+VSL RFG+ AEGHS+TKME
Sbjct: 301 LRIKTGGSRNDNVFWETTMESTIQDYRTIDGVNIAHAGKTTVSLSRFGDGAEGHSKTKME 360

Query: 361 EIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTI--RCTAAASKICSS 420
           EIW+IEEVDFNIKGLSM+FFLPPSDLKKEEE +  I S+ KFPL +  R   A S+I  S
Sbjct: 361 EIWKIEEVDFNIKGLSMEFFLPPSDLKKEEEEIGAIASDEKFPLAMRRRSAGAGSRIYPS 420

Query: 421 RVAAIDVDEISEGSSNQSDEDEDE 441
           RVAA+D DE SEGSS +SDED+D+
Sbjct: 421 RVAAVDDDE-SEGSS-RSDEDQDQ 442

BLAST of MS003423 vs. ExPASy TrEMBL
Match: A0A6J1JRQ6 (uncharacterized protein LOC111489190 OS=Cucurbita maxima OX=3661 GN=LOC111489190 PE=4 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.6e-207
Identity = 372/443 (83.97%), Postives = 404/443 (91.20%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHK-SHVNSM 60
           MRKLCPNFDREDGLDTVLEVPIPEEMFSC T K+HAISWQAMKSWVKS   +K SHV S+
Sbjct: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCPTYKSHAISWQAMKSWVKSSYYNKPSHVASI 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVG 120
           A+LFGGRNAEIQLLLGVVGAPLIPLP+ F H+ ITRNIKDNPIEASMAKYIVQQY+AAVG
Sbjct: 61  ASLFGGRNAEIQLLLGVVGAPLIPLPIAFHHQSITRNIKDNPIEASMAKYIVQQYIAAVG 120

Query: 121 GEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGG-GGGEMGGFVVWQKRP 180
           GEHALNSI SMYAMGKVKMVASEF+SGEG  NGK +KAKNGK G   GEMG FV+WQKRP
Sbjct: 121 GEHALNSIHSMYAMGKVKMVASEFTSGEGYFNGKPMKAKNGKDGISSGEMGSFVIWQKRP 180

Query: 181 ELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN 240
           +LWCLE+MLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN
Sbjct: 181 DLWCLEMMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSN 240

Query: 241 SSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHL 300
           S+CIGEKT+N EDCFILKLEAES+VLRARSSS VEIIRHTVWGYFSQRTGLLV LEDSHL
Sbjct: 241 STCIGEKTMNGEDCFILKLEAESSVLRARSSSGVEIIRHTVWGYFSQRTGLLVHLEDSHL 300

Query: 301 LRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKME 360
           LRIK GGSRND +FWETTME+ I+DYRTIDGVNIAHAGKT+VSL RFG+ AEGHS+TKME
Sbjct: 301 LRIKTGGSRNDDVFWETTMESTIRDYRTIDGVNIAHAGKTTVSLSRFGDGAEGHSKTKME 360

Query: 361 EIWEIEEVDFNIKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTI-RCTAAASKICSSR 420
           EIW+IEEVDFNIKGLSM+FFLPPSDLKKEEE + V+TS+ KFP T+ R + A S+I SSR
Sbjct: 361 EIWKIEEVDFNIKGLSMEFFLPPSDLKKEEE-IGVVTSDEKFPSTMRRASGAGSRIYSSR 420

Query: 421 VAAIDVDEISEGSSNQSDEDEDE 441
           VAA+D DE SEGSS +SDEDED+
Sbjct: 421 VAAVDDDE-SEGSS-RSDEDEDD 440

BLAST of MS003423 vs. TAIR 10
Match: AT5G05840.1 (Protein of unknown function (DUF620) )

HSP 1 Score: 576.2 bits (1484), Expect = 2.2e-164
Identity = 302/445 (67.87%), Postives = 360/445 (80.90%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKS-WVK--SHNDHKSHVN 60
           MRKLCPN++ EDGL+TVLEVP+PEE+F+ +  K     W  MKS W K  +     +   
Sbjct: 1   MRKLCPNYNLEDGLETVLEVPMPEELFAASKTKP---GWNQMKSYWSKPTATATGTATAT 60

Query: 61  SMAALFGGRNAEIQLLLGVVGAPLIPLPVPFDH-----RPITRNIKDNPIEASMAKYIVQ 120
           +M  LFGGRNAEIQLLLGVVGAPLIPLPV  DH      PI ++IKD P+E SMA+YIV+
Sbjct: 61  NMTRLFGGRNAEIQLLLGVVGAPLIPLPVQPDHHNDYENPIHKDIKDQPLEMSMAQYIVK 120

Query: 121 QYVAAVGGEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFV 180
           QY+AAVGG+ ALN++ESMYAMGKV+M ASEF +GEGSLN K++KA++ K  GGGE+GGFV
Sbjct: 121 QYIAAVGGDRALNAVESMYAMGKVRMTASEFCTGEGSLNSKMVKARSIK-SGGGEVGGFV 180

Query: 181 VWQKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKST 240
           +WQK  ELWCLEL++SGCKISAGSD KVAWRQTPWH SHASRGPPRPLRRFLQGLDPKST
Sbjct: 181 LWQKGIELWCLELVVSGCKISAGSDAKVAWRQTPWHPSHASRGPPRPLRRFLQGLDPKST 240

Query: 241 ATLFSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQ 300
           A LF+ S C+GEK INDEDCFILKL+AE + L+ARSSS+VEIIRHTVWG FSQRTGLL+Q
Sbjct: 241 ANLFARSVCMGEKKINDEDCFILKLDAEPSALKARSSSNVEIIRHTVWGCFSQRTGLLIQ 300

Query: 301 LEDSHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGH 360
           LEDSHLLRIKA    ++SIFWETTME+LIQDYRT+DG+ +AHAGK+SVSLFRFGE+++ H
Sbjct: 301 LEDSHLLRIKA--QDDNSIFWETTMESLIQDYRTVDGILVAHAGKSSVSLFRFGENSDNH 360

Query: 361 SRTKMEEIWEIEEVDFNIKGLSMDFFLPPSDLKK---EEEGV--AVITSNGKFPLTIRCT 420
           SRT+MEE WEIEE+DFNIKGLSMD FLPPSDLKK   EEE +   +  +N K P+ IR  
Sbjct: 361 SRTRMEETWEIEEMDFNIKGLSMDCFLPPSDLKKDDDEEEEIECGLAANNEKLPMKIR-- 420

Query: 421 AAASKICSSRVAAI----DVDEISE 429
           +A+ +I SS+V AI    D  E++E
Sbjct: 421 SASLRISSSKVLAIVEEEDESEVTE 437

BLAST of MS003423 vs. TAIR 10
Match: AT3G55720.1 (Protein of unknown function (DUF620) )

HSP 1 Score: 480.3 bits (1235), Expect = 1.6e-135
Identity = 250/437 (57.21%), Postives = 318/437 (72.77%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMK-SWVKSHNDHKSHVNSM 60
           MR LCPNFDREDGL+TVLEVP+PEE+F  + NK+ A  W+++K S ++S  D+ S   S+
Sbjct: 1   MRTLCPNFDREDGLETVLEVPMPEELFCSDNNKSGA--WRSVKSSLLRSPPDNSS---SL 60

Query: 61  AALFGGRNAEIQLLLGVVGAPLIPLPVPFDH----RPITRNIKDNPIEASMAKYIVQQYV 120
           A LFGGR+++IQ+LLG+VGAP IPLP+  D      PI+  IK+  IE++MAKYIV+QY 
Sbjct: 61  ATLFGGRDSQIQMLLGIVGAPSIPLPISSDQAKIDHPISNLIKNQSIESAMAKYIVKQYT 120

Query: 121 AAVGGEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAK------NGKGGGGGEMG 180
           AA GGE AL+++ESMYAMGKVKM  +EF + + +LNGK  K        N   G GGEMG
Sbjct: 121 AAAGGEMALDAVESMYAMGKVKMGVTEFCAAK-TLNGKRKKKMVRIRNVNNNNGNGGEMG 180

Query: 181 GFVVWQKRPELWCLELMLSGCKISAGSDGKVAWRQTPW-HHSHASRGPPRPLRRFLQGLD 240
           GFV+W+K    W LEL++SGCK+SAG DG V WRQ+PW  HSHAS  P  PLRRFLQGLD
Sbjct: 181 GFVLWKKGSSQWSLELVVSGCKVSAGCDGNVDWRQSPWLAHSHASNEPSGPLRRFLQGLD 240

Query: 241 PKSTATLFSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTG 300
           PK+TA LF+ S C+GEK +N+E+CF+LKLE + + L++RS S +E ++HTVWG F QRTG
Sbjct: 241 PKTTANLFAGSVCVGEKAVNNEECFVLKLETQPSGLKSRSKSGMETVKHTVWGCFGQRTG 300

Query: 301 LLVQLEDSHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGES 360
           LLVQLED++L+RIK G    D + WETT ETLIQDY++IDG+ IAH GKT VSL R  ES
Sbjct: 301 LLVQLEDTYLVRIKTGLEDEDMVLWETTSETLIQDYKSIDGIQIAHRGKTRVSLLRLDES 360

Query: 361 AEGHSRTKMEEIWEIEEVDFNIKGLSMDFFLPPSDL---KKEEEGVAVITSNGKFPLTIR 420
            E HS+T MEE WEIEEV FN+KGLS DFFLPP DL   ++EE G +         L ++
Sbjct: 361 LESHSKTTMEESWEIEEVGFNVKGLSSDFFLPPGDLCIKEEEETGFSFGDYTSPMLLPLK 420

Query: 421 CTAAASKICSSRVAAID 423
            +  + KI SS+V AI+
Sbjct: 421 VSTTSWKIKSSKVTAIE 431

BLAST of MS003423 vs. TAIR 10
Match: AT5G66740.1 (Protein of unknown function (DUF620) )

HSP 1 Score: 417.9 bits (1073), Expect = 9.9e-117
Identity = 214/388 (55.15%), Postives = 279/388 (71.91%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMA 60
           MRKLCPN D++DGL+TVLEVPIPEEMFS   N   A+ WQ M +W+K+    K     +A
Sbjct: 1   MRKLCPNIDKDDGLETVLEVPIPEEMFSGMGNNV-ALRWQNMMTWMKAQTSDKWSQPLIA 60

Query: 61  ALFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGG 120
           A    R  E++ LL +VG+PLIPL V   H  + + +KD  I+AS AKYIVQQY+AA GG
Sbjct: 61  A----RINELRFLLYLVGSPLIPLQVQVGH-SVHKPVKDCSIQASTAKYIVQQYIAATGG 120

Query: 121 EHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPEL 180
             ALN++ SM   G+VKM ASEF  G+ S  G  LK+ +       EMGGFV+WQK P+L
Sbjct: 121 PQALNAVNSMCVTGQVKMTASEFHQGDDS--GVNLKSND-------EMGGFVLWQKDPDL 180

Query: 181 WCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSS 240
           WCLEL++SGCK+  GS+G+++WR +    + AS G PRPLRRFLQGLDP+STA LF +++
Sbjct: 181 WCLELVVSGCKVICGSNGRLSWRHSSNQQTPASTGTPRPLRRFLQGLDPRSTANLFLDAT 240

Query: 241 CIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLR 300
           CIGEK IN EDCFILKLE    V  A+S  + EII HT+WGYFSQR+GLL+Q EDS LLR
Sbjct: 241 CIGEKIINGEDCFILKLETSPAVREAQSGPNFEIIHHTIWGYFSQRSGLLIQFEDSRLLR 300

Query: 301 IKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEI 360
           ++     ++ +FWET+ E+++ DYR +D VNIAH GKTSV++FR+GE++  H R +M E 
Sbjct: 301 MRT--KEDEDVFWETSAESVMDDYRYVDNVNIAHGGKTSVTVFRYGEASANH-RRQMTEK 360

Query: 361 WEIEEVDFNIKGLSMDFFLPPSDLKKEE 389
           W IEEVDFN+ GLS+D FLPP++L+ E+
Sbjct: 361 WRIEEVDFNVWGLSVDHFLPPANLQIEK 370

BLAST of MS003423 vs. TAIR 10
Match: AT1G75160.1 (Protein of unknown function (DUF620) )

HSP 1 Score: 403.7 bits (1036), Expect = 1.9e-112
Identity = 209/400 (52.25%), Postives = 277/400 (69.25%), Query Frame = 0

Query: 1   MRKLCPNFDREDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSH-------NDHK 60
           MRKLCPN DREDGL+TVLEVP+PEEMF+   +      W+ M + +K+H        D +
Sbjct: 3   MRKLCPNLDREDGLETVLEVPVPEEMFTKMGSNAQG-RWRNMHALMKAHAVVTAVATDVR 62

Query: 61  SHVNSMAA------LFGGRNAEIQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMA 120
           +  +S +       L    + E   LL +VG+PLIP  VP +   ++R I D  IEAS A
Sbjct: 63  TPASSSSMSNVNMHLQSKSDNEFVALLKIVGSPLIPFHVPLEF-CLSRPINDTSIEASTA 122

Query: 121 KYIVQQYVAAVGGEHALNSIESMYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGE 180
           KYIVQQYVAA GG  ALN+++SMYA+G+V+M  SE  +GE    G  ++     G G  E
Sbjct: 123 KYIVQQYVAACGGPTALNAVKSMYAVGQVRMQGSEMVAGEDEGTGTPVRL----GKGSFE 182

Query: 181 MGGFVVWQKRPELWCLELMLSGCKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGL 240
           +GGFV+WQK P LW LEL++SG KISAGSDGKVAW Q+    S A RGPPRPLRRF QGL
Sbjct: 183 VGGFVLWQKNPNLWFLELVVSGFKISAGSDGKVAWNQSSTQPSQAHRGPPRPLRRFFQGL 242

Query: 241 DPKSTATLFSNSSCIGEKTINDEDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRT 300
           DP+ TA+LF ++ CIGE+ +N EDCF+LK+E  S +L+A+ S + E+I HTVWGYFSQRT
Sbjct: 243 DPRCTASLFLDAVCIGEQPVNGEDCFVLKVETPSDILKAQCSPNTEVIHHTVWGYFSQRT 302

Query: 301 GLLVQLEDSHLLRIKAGGSRNDSIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGE 360
           GLLV+  D+ L+R+K+G  +ND +FWET+ME++I DY  +D VNIAH G+T  +L+R+G 
Sbjct: 303 GLLVKFGDTKLVRVKSGRGKNDGVFWETSMESIIDDYIFVDAVNIAHGGQTVTTLYRYGG 362

Query: 361 SAEGHSRTKMEEIWEIEEVDFNIKGLSMDFFLPPSDLKKE 388
           +   + R ++EE W IEEVDFNI GL ++ FLPPSD+  +
Sbjct: 363 AV--NHRRRIEEKWRIEEVDFNICGLCLESFLPPSDINND 394

BLAST of MS003423 vs. TAIR 10
Match: AT3G19540.1 (Protein of unknown function (DUF620) )

HSP 1 Score: 352.4 bits (903), Expect = 5.1e-97
Identity = 189/416 (45.43%), Postives = 258/416 (62.02%), Query Frame = 0

Query: 10  REDGLDTVLEVPIPEEMFSCNTNKTHAISWQAMKSWVKSHNDHKSHVNSMAALFGGRNAE 69
           R   L  V+E P P+E                +  WVK      S   S+AA    R  +
Sbjct: 73  RSGSLRPVMEGPDPDEGGGGGNIGESKRLGSGLGHWVKG---QLSRAPSVAATAAYRRND 132

Query: 70  IQLLLGVVGAPLIPLPVPFDHRPITRNIKDNPIEASMAKYIVQQYVAAVGGEHALNSIES 129
           ++LLLGV+GAPL P+ V         +IK+ PIE S A+YI+QQY AA GG+   NSI++
Sbjct: 133 LRLLLGVMGAPLAPIHVSSSDPLPHLSIKNTPIETSSAQYILQQYTAASGGQKLQNSIKN 192

Query: 130 MYAMGKVKMVASEFSSGEGSLNGKVLKAKNGKGGGGGEMGGFVVWQKRPELWCLELMLSG 189
            YAMGK+KM+ SE  +          +    +     E GGFV+WQ  P++W +EL + G
Sbjct: 193 AYAMGKLKMITSELET--------ATRTVRNRNPSKAETGGFVLWQMNPDMWYVELAVGG 252

Query: 190 CKISAGSDGKVAWRQTPWHHSHASRGPPRPLRRFLQGLDPKSTATLFSNSSCIGEKTIND 249
            K+ AG +GK+ WR TPW  SH ++GP RPLRR LQGLDP++TA +F+ + CIGEK +N 
Sbjct: 253 SKVRAGCNGKLVWRHTPWLGSHTAKGPVRPLRRGLQGLDPRTTAAMFAEAKCIGEKKVNG 312

Query: 250 EDCFILKLEAESTVLRARSSSSVEIIRHTVWGYFSQRTGLLVQLEDSHLLRIKAGGSRND 309
           EDCFILKL  +   L+ARS    EIIRH ++GYFSQ+TGLLV +EDSHL RI++ G   +
Sbjct: 313 EDCFILKLCTDPETLKARSEGPAEIIRHVLFGYFSQKTGLLVHIEDSHLTRIQSNG--GE 372

Query: 310 SIFWETTMETLIQDYRTIDGVNIAHAGKTSVSLFRFGESAEGHSRTKMEEIWEIEEVDFN 369
           ++FWETT  + + DYR ++G+ IAH+G + V+LFRFGE A  H+RTKMEE W IEEV FN
Sbjct: 373 TVFWETTYNSSLDDYRQVEGIMIAHSGHSVVTLFRFGEVATSHTRTKMEESWTIEEVAFN 432

Query: 370 IKGLSMDFFLPPSDLKKEEEGVAVITSNGKFPLTIR---CTAAASKICSSRVAAID 423
           + GLS+D F+PP+DLK        +T + ++P   R    T A S    ++VAA++
Sbjct: 433 VPGLSLDCFIPPADLK-----TGSLTESCEYPQEERGKNNTLALSAAHRAKVAALE 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133661.15.8e-25299.32uncharacterized protein LOC111006191 [Momordica charantia][more]
XP_022957502.11.3e-22490.16uncharacterized protein LOC111458877 [Cucurbita moschata][more]
XP_022990734.11.3e-22490.45uncharacterized protein LOC111487531 [Cucurbita maxima][more]
KAG6602636.15.6e-22389.29hypothetical protein SDJN03_07869, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038886072.13.5e-21787.00uncharacterized protein LOC120076338 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1BVR52.8e-25299.32uncharacterized protein LOC111006191 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1JSU86.5e-22590.45uncharacterized protein LOC111487531 OS=Cucurbita maxima OX=3661 GN=LOC111487531... [more]
A0A6J1GZA96.5e-22590.16uncharacterized protein LOC111458877 OS=Cucurbita moschata OX=3662 GN=LOC1114588... [more]
A0A6J1FGT47.2e-20883.33uncharacterized protein LOC111445268 OS=Cucurbita moschata OX=3662 GN=LOC1114452... [more]
A0A6J1JRQ63.6e-20783.97uncharacterized protein LOC111489190 OS=Cucurbita maxima OX=3661 GN=LOC111489190... [more]
Match NameE-valueIdentityDescription
AT5G05840.12.2e-16467.87Protein of unknown function (DUF620) [more]
AT3G55720.11.6e-13557.21Protein of unknown function (DUF620) [more]
AT5G66740.19.9e-11755.15Protein of unknown function (DUF620) [more]
AT1G75160.11.9e-11252.25Protein of unknown function (DUF620) [more]
AT3G19540.15.1e-9745.43Protein of unknown function (DUF620) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 439..440
NoneNo IPR availablePANTHERPTHR31300:SF30EMB|CAB81597.1coord: 1..427
IPR006873Protein of unknown function DUF620PFAMPF04788DUF620coord: 130..381
e-value: 2.4E-122
score: 407.0
IPR006873Protein of unknown function DUF620PANTHERPTHR31300LIPASEcoord: 1..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003423.1MS003423.1mRNA