MS003405 (gene) Bitter gourd (TR) v1

BLAST of MS003405 vs. NCBI nr
Match: XP_022134506.1 (nematode resistance protein-like HSPRO2 [Momordica charantia])

HSP 1 Score: 938.3 bits (2424), Expect = 2.5e-269
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND 60
           MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND
Sbjct: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND 60

Query: 61  LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ 120
           LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ
Sbjct: 61  LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ 120

Query: 121 IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL 180
           IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL
Sbjct: 121 IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL 180

Query: 181 LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR 240
           LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR
Sbjct: 181 LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR 240

Query: 241 KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW 300
           KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW
Sbjct: 241 KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW 300

Query: 301 KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI 360
           KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI
Sbjct: 301 KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI 360

Query: 361 LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS 420
           LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS
Sbjct: 361 LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS 420

Query: 421 LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL
Sbjct: 421 LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 475

BLAST of MS003405 vs. NCBI nr
Match: XP_038885639.1 (nematode resistance protein-like HSPRO2 [Benincasa hispida])

HSP 1 Score: 854.4 bits (2206), Expect = 4.8e-244
Identity = 430/480 (89.58%), Postives = 459/480 (95.62%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDKNTRSPKL-----ISNKQLNLSLPVSFRSSELSTASPSSCSAYEHY 60
           MVDLGWKANMVSSDK T + K      I+N +LNLSLPVSFR+SELSTASPSSCS+YE Y
Sbjct: 1   MVDLGWKANMVSSDKTTTTTKSSKIIPIANNKLNLSLPVSFRTSELSTASPSSCSSYEQY 60

Query: 61  LRLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLES 120
           LRLNDLRKLWSS++FPEWR+ESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLES
Sbjct: 61  LRLNDLRKLWSSKEFPEWRNESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLES 120

Query: 121 LTTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRT 180
           LTT+QIQLIAMICEDDEEDGEARGR+PIVDLSSSDGVITR+GSSAEVWK+ GEATVV+RT
Sbjct: 121 LTTSQIQLIAMICEDDEEDGEARGRVPIVDLSSSDGVITRDGSSAEVWKIHGEATVVNRT 180

Query: 181 SEASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNE 240
           SE+SLLPRLASWQSSEDIAQ+I Y VECEMRRC YTLGLGEPNLAGKPNLDYDLIC+PNE
Sbjct: 181 SESSLLPRLASWQSSEDIAQLIQYSVECEMRRCSYTLGLGEPNLAGKPNLDYDLICKPNE 240

Query: 241 LHSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYL 300
           LHSLRK+PY +QIENYENQTVYTTHQILESWI+AA E+LKRI ERIE+K+FA AASDCYL
Sbjct: 241 LHSLRKSPY-EQIENYENQTVYTTHQILESWIYAAHEVLKRIAERIEKKNFAGAASDCYL 300

Query: 301 LERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKH 360
           +ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRST LVEITKQCKDLKH
Sbjct: 301 MERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTKLVEITKQCKDLKH 360

Query: 361 KVPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLV 420
           KVPFIL+VEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIE+ IK+FFF+YKQVLV
Sbjct: 361 KVPFILDVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIESAIKKFFFAYKQVLV 420

Query: 421 IVMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           +VMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGD+HGVFGSDRRTRMKL
Sbjct: 421 MVMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDLHGVFGSDRRTRMKL 479

BLAST of MS003405 vs. NCBI nr
Match: XP_011656406.1 (nematode resistance protein-like HSPRO2 [Cucumis sativus] >KGN45776.1 hypothetical protein Csa_004812 [Cucumis sativus])

HSP 1 Score: 847.0 bits (2187), Expect = 7.7e-242
Identity = 426/482 (88.38%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-----NTRSPKLIS--NKQLNLSLPVSFRSSELSTASPSSCSAYE 60
           MVDLGWKANMVSSDK      T+S K+I   N +LNLSLPVSFRSSELSTASPSSCS+YE
Sbjct: 1   MVDLGWKANMVSSDKTTTATTTKSSKVIPILNNKLNLSLPVSFRSSELSTASPSSCSSYE 60

Query: 61  HYLRLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL 120
           HYLRLN LRKLWSS++FPEWR+ESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL
Sbjct: 61  HYLRLNHLRKLWSSKEFPEWRNESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL 120

Query: 121 ESLTTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVS 180
           +SLTTTQIQLIAMICEDDEEDGE+RGR+PIVDLSSSDG+ITR+GSSAEVWK+ GEATVV+
Sbjct: 121 QSLTTTQIQLIAMICEDDEEDGESRGRVPIVDLSSSDGMITRDGSSAEVWKIHGEATVVN 180

Query: 181 RTSEASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRP 240
           RTSE+SLLPRLASWQSSEDIAQ+I Y VECEMRRCPYTLGLGEPNLAGKPNLDYDLIC+P
Sbjct: 181 RTSESSLLPRLASWQSSEDIAQLIQYSVECEMRRCPYTLGLGEPNLAGKPNLDYDLICKP 240

Query: 241 NELHSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDC 300
           NELHSLRK+PY +QIENYENQTVYTTHQILESWI++A ELLKRI ERIE+KDFA AA DC
Sbjct: 241 NELHSLRKSPY-EQIENYENQTVYTTHQILESWIYSAHELLKRIEERIEKKDFAGAARDC 300

Query: 301 YLLERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL 360
           Y++ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL
Sbjct: 301 YMMERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL 360

Query: 361 KHKVPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQV 420
           KHKVP IL+VEVDPMGGPRIQEAAMKLYSEK+E EKIHLLQALQAIE+GIK+FFF+YKQV
Sbjct: 361 KHKVPLILDVEVDPMGGPRIQEAAMKLYSEKQESEKIHLLQALQAIESGIKKFFFAYKQV 420

Query: 421 LVIVMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRM 476
           LV+VMGSLE KGNRVVVSSNSDDSLSQIFLE TYFPSLDAAKTFLGD+HGVFGSDRRTRM
Sbjct: 421 LVMVMGSLEGKGNRVVVSSNSDDSLSQIFLEPTYFPSLDAAKTFLGDLHGVFGSDRRTRM 480

BLAST of MS003405 vs. NCBI nr
Match: XP_022993360.1 (nematode resistance protein-like HSPRO2 [Cucurbita maxima])

HSP 1 Score: 844.7 bits (2181), Expect = 3.8e-241
Identity = 426/476 (89.50%), Postives = 452/476 (94.96%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-NTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLN 60
           MVDLGWKANMVSSDK  T+SPKLI    L+LSLP+SFRSS++STASPSSCS+Y+HYLRLN
Sbjct: 1   MVDLGWKANMVSSDKTTTKSPKLIPTANLSLSLPLSFRSSDISTASPSSCSSYDHYLRLN 60

Query: 61  DLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTT 120
           DLRKLWSS++FPEW SESVLKPGLQALEITFRFIS VLSDPRPY NR EWKRKLESLTT+
Sbjct: 61  DLRKLWSSKEFPEWTSESVLKPGLQALEITFRFISIVLSDPRPYVNRWEWKRKLESLTTS 120

Query: 121 QIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEAS 180
           QIQLIAMI EDDEED EARGR+PIVDLSSS+GVITREGSSAEVWK+RGEATVVSRTSE S
Sbjct: 121 QIQLIAMIFEDDEEDDEARGRVPIVDLSSSNGVITREGSSAEVWKIRGEATVVSRTSELS 180

Query: 181 LLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSL 240
           LLPRLASWQSSEDIAQMI Y VECEMRRCPYTLGLGEPNLAGKPN+DYD ICRPNELHSL
Sbjct: 181 LLPRLASWQSSEDIAQMIQYTVECEMRRCPYTLGLGEPNLAGKPNIDYDQICRPNELHSL 240

Query: 241 RKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERI 300
           RK+PYD QIENYENQTVYTTHQILESWI+AA ELLKRI ERIE K+FARAA DCYL+ERI
Sbjct: 241 RKSPYD-QIENYENQTVYTTHQILESWIYAAHELLKRIAERIEMKNFARAAKDCYLMERI 300

Query: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360
           WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF
Sbjct: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360

Query: 361 ILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMG 420
           IL+VEVDPMGGPRIQEAAM+LYSEKRE EKIHLLQALQ+IE+ IK+FFF+YKQVLV+VMG
Sbjct: 361 ILDVEVDPMGGPRIQEAAMRLYSEKREIEKIHLLQALQSIESAIKKFFFAYKQVLVMVMG 420

Query: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGD+H VFGSDRRTRMKL
Sbjct: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDMHSVFGSDRRTRMKL 475

BLAST of MS003405 vs. NCBI nr
Match: KAG6578444.1 (Nematode resistance protein-like HSPRO2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 842.0 bits (2174), Expect = 2.5e-240
Identity = 425/476 (89.29%), Postives = 451/476 (94.75%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-NTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLN 60
           MVDLGWKANMVSSDK  T+SPKLI    L+LSLP+SFRSS++STASPSSCS+Y+HYLRLN
Sbjct: 1   MVDLGWKANMVSSDKTTTKSPKLIPTANLSLSLPLSFRSSDISTASPSSCSSYDHYLRLN 60

Query: 61  DLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTT 120
           DLRKLWSS++FPEW SESVLKPGLQALEITFRFIS VLSDPRPY NR EWKRKLESLTTT
Sbjct: 61  DLRKLWSSKEFPEWTSESVLKPGLQALEITFRFISIVLSDPRPYVNRWEWKRKLESLTTT 120

Query: 121 QIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEAS 180
           QIQLIAMI EDDEED EARGR+PIVDLSSS+GVITREGSSAEVWK+RGEATVVSRTSE S
Sbjct: 121 QIQLIAMIFEDDEEDDEARGRVPIVDLSSSNGVITREGSSAEVWKIRGEATVVSRTSELS 180

Query: 181 LLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSL 240
           LLPRLASWQSSEDIAQMI Y VECEMRRCPYTLGLGEPNLAGKPN+DYDLICRPNELHSL
Sbjct: 181 LLPRLASWQSSEDIAQMIQYSVECEMRRCPYTLGLGEPNLAGKPNIDYDLICRPNELHSL 240

Query: 241 RKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERI 300
           RK  YD QIENYENQTVYTTHQILESWI+AA ELLKRI ERIE K+FA+AA DCYL+ERI
Sbjct: 241 RKNLYD-QIENYENQTVYTTHQILESWIYAAHELLKRIAERIEMKNFAKAAKDCYLMERI 300

Query: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360
           WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF
Sbjct: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360

Query: 361 ILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMG 420
           IL+VEVDPMGGPRIQEAAM+LYSEKRE EKIHLLQALQ+IE+ IK+FFF+YKQVLV+VMG
Sbjct: 361 ILDVEVDPMGGPRIQEAAMRLYSEKREIEKIHLLQALQSIESAIKKFFFAYKQVLVMVMG 420

Query: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGD+H V+GSDRRTRMKL
Sbjct: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDMHSVYGSDRRTRMKL 475

BLAST of MS003405 vs. ExPASy Swiss-Prot
Match: O04203 (Nematode resistance protein-like HSPRO2 OS=Arabidopsis thaliana OX=3702 GN=HSPRO2 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-141
Identity = 264/470 (56.17%), Postives = 327/470 (69.57%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFR----SSELSTASPSSCSAYEHYL 60
           MVD+ WK  MVSSD    SPKL S  +L++++P  F+    SS +S ++P+ CSAYE YL
Sbjct: 1   MVDMDWKRKMVSSDL-PNSPKLSS--KLHVTIPSPFKIVPVSSPISCSAPALCSAYELYL 60

Query: 61  RLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESL 120
           RL +LRKLWSSRDFP+W SE +LKP LQALEI+FR +  V SD RPY N REW R+L+SL
Sbjct: 61  RLPELRKLWSSRDFPQWTSEPILKPALQALEISFRLVFAVCSDTRPYINHREWNRRLDSL 120

Query: 121 TTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTS 180
            T QIQL+A ICED+EE+G                             +  EA V    S
Sbjct: 121 ITKQIQLVAAICEDEEEEG-----------------------------ISAEAPVGGGRS 180

Query: 181 EASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNEL 240
             SLLP+LA+W+ SE + + ILY ++ EM RC YTLGLGE N+AGKPNL YD ICRPNE+
Sbjct: 181 SLSLLPQLATWRRSEALGKKILYTIDNEMSRCKYTLGLGEQNIAGKPNLRYDAICRPNEI 240

Query: 241 HSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLL 300
           +SL+  PY   I+N+ENQT+Y  HQILESWI+A+  LL RI   IE + F +A++D YLL
Sbjct: 241 YSLKDNPYADHIDNHENQTLYIIHQILESWIYASGNLLNRIVSSIEEEKFGKASNDVYLL 300

Query: 301 ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHK 360
           E+IWK+L EIEDLH+LMDP+DFLKLK QL IKS  +++AFCFRS  LVE+ K  KDL+ K
Sbjct: 301 EKIWKILAEIEDLHMLMDPEDFLKLKKQLQIKSTGKNDAFCFRSKGLVEMMKMSKDLRQK 360

Query: 361 VPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVI 420
           VP +L VEVDP GGPR+QEAAMKLY+ K E +KIHLLQ +QA+EA  K FFF Y+Q++  
Sbjct: 361 VPAVLAVEVDPTGGPRLQEAAMKLYARKTECDKIHLLQGMQAVEAAAKSFFFGYRQLVAA 420

Query: 421 VMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFG 467
           +MGS E        S  S DSLSQIF+E TYFPSLDAAKTFLG+     G
Sbjct: 421 MMGSAEM---NATASQESCDSLSQIFMEPTYFPSLDAAKTFLGEFWSHLG 435

BLAST of MS003405 vs. ExPASy Swiss-Prot
Match: Q9LY61 (Nematode resistance protein-like HSPRO1 OS=Arabidopsis thaliana OX=3702 GN=HSPRO1 PE=1 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 4.4e-131
Identity = 249/444 (56.08%), Postives = 313/444 (70.50%), Query Frame = 0

Query: 21  KLISNKQLNLSLP----VSFRSSELSTASPSSCSAYEHYLRLNDLRKLWSSRDFPEWRSE 80
           K++S K L++++P    +S  SS +S++S ++CSAYE YLRL +LR LWSS  FP W SE
Sbjct: 9   KMVSPK-LHVTIPEPCKLSSVSSPISSSSSAACSAYELYLRLPELRNLWSSLYFPHWISE 68

Query: 81  SVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQIQLIAMICEDDEEDGE 140
            VLKP LQALEITFR I TV SD RPY NRREW R+L+SLTT+QI+++A IC D++   E
Sbjct: 69  PVLKPALQALEITFRLILTVASDTRPYINRREWIRRLDSLTTSQIKIVAAICGDEDNYEE 128

Query: 141 ARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASLLPRLASWQSSEDIAQM 200
                P+     S+G           W            S  SLL  +A+ ++SE + Q 
Sbjct: 129 NVSAAPV-----SNG-----------W------------SSLSLLSEIATCRTSESVGQK 188

Query: 201 ILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLRKTPYDQQIENYENQTV 260
           IL  +E EMR C YTLGLGEPNLAGKP L YD +C P ELHSL+  PY   IEN ENQ +
Sbjct: 189 ILSTIENEMRWCKYTLGLGEPNLAGKPYLQYDAVCLPEELHSLKNNPYADHIENQENQML 248

Query: 261 YTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIWKLLQEIEDLHLLMDPD 320
           YT+HQILESWI+ +  LL RI  RIE   F +A+SD YLLERIWKLL EIEDLH+LMDP+
Sbjct: 249 YTSHQILESWIYVSVNLLYRIESRIEEGKFEKASSDVYLLERIWKLLSEIEDLHILMDPE 308

Query: 321 DFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFILEVEVDPMGGPRIQEA 380
           DFLK+K QL IKS   ++AFCFRS  LVE+ K  K+L+ KVP +LEVEVDP GGPR+QEA
Sbjct: 309 DFLKVKKQLQIKSTFPNDAFCFRSKGLVEMAKMSKELRQKVPAVLEVEVDPTGGPRLQEA 368

Query: 381 AMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGSLEAKGNRVVVSSNSDD 440
           AMKLYS K E+EKIHLLQ +QA+E+  KRFFF Y++++  ++G+ EA  NR V +  S D
Sbjct: 369 AMKLYSRKTEYEKIHLLQGMQAVESAAKRFFFGYQKLVAAMIGNAEANANRTVANHESYD 423

Query: 441 SLSQIFLESTYFPSLDAAKTFLGD 461
           SL+Q+F+E  Y+PSLDAAKTFLG+
Sbjct: 429 SLTQVFMEPPYYPSLDAAKTFLGE 423

BLAST of MS003405 vs. ExPASy TrEMBL
Match: A0A6J1BXZ4 (nematode resistance protein-like HSPRO2 OS=Momordica charantia OX=3673 GN=LOC111006732 PE=4 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND 60
           MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND
Sbjct: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLND 60

Query: 61  LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ 120
           LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ
Sbjct: 61  LRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQ 120

Query: 121 IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL 180
           IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL
Sbjct: 121 IQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASL 180

Query: 181 LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR 240
           LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR
Sbjct: 181 LPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLR 240

Query: 241 KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW 300
           KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW
Sbjct: 241 KTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIW 300

Query: 301 KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI 360
           KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI
Sbjct: 301 KLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFI 360

Query: 361 LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS 420
           LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS
Sbjct: 361 LEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGS 420

Query: 421 LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL
Sbjct: 421 LEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 475

BLAST of MS003405 vs. ExPASy TrEMBL
Match: A0A0A0KDB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G010030 PE=4 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.7e-242
Identity = 426/482 (88.38%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-----NTRSPKLIS--NKQLNLSLPVSFRSSELSTASPSSCSAYE 60
           MVDLGWKANMVSSDK      T+S K+I   N +LNLSLPVSFRSSELSTASPSSCS+YE
Sbjct: 1   MVDLGWKANMVSSDKTTTATTTKSSKVIPILNNKLNLSLPVSFRSSELSTASPSSCSSYE 60

Query: 61  HYLRLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL 120
           HYLRLN LRKLWSS++FPEWR+ESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL
Sbjct: 61  HYLRLNHLRKLWSSKEFPEWRNESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKL 120

Query: 121 ESLTTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVS 180
           +SLTTTQIQLIAMICEDDEEDGE+RGR+PIVDLSSSDG+ITR+GSSAEVWK+ GEATVV+
Sbjct: 121 QSLTTTQIQLIAMICEDDEEDGESRGRVPIVDLSSSDGMITRDGSSAEVWKIHGEATVVN 180

Query: 181 RTSEASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRP 240
           RTSE+SLLPRLASWQSSEDIAQ+I Y VECEMRRCPYTLGLGEPNLAGKPNLDYDLIC+P
Sbjct: 181 RTSESSLLPRLASWQSSEDIAQLIQYSVECEMRRCPYTLGLGEPNLAGKPNLDYDLICKP 240

Query: 241 NELHSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDC 300
           NELHSLRK+PY +QIENYENQTVYTTHQILESWI++A ELLKRI ERIE+KDFA AA DC
Sbjct: 241 NELHSLRKSPY-EQIENYENQTVYTTHQILESWIYSAHELLKRIEERIEKKDFAGAARDC 300

Query: 301 YLLERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL 360
           Y++ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL
Sbjct: 301 YMMERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDL 360

Query: 361 KHKVPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQV 420
           KHKVP IL+VEVDPMGGPRIQEAAMKLYSEK+E EKIHLLQALQAIE+GIK+FFF+YKQV
Sbjct: 361 KHKVPLILDVEVDPMGGPRIQEAAMKLYSEKQESEKIHLLQALQAIESGIKKFFFAYKQV 420

Query: 421 LVIVMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRM 476
           LV+VMGSLE KGNRVVVSSNSDDSLSQIFLE TYFPSLDAAKTFLGD+HGVFGSDRRTRM
Sbjct: 421 LVMVMGSLEGKGNRVVVSSNSDDSLSQIFLEPTYFPSLDAAKTFLGDLHGVFGSDRRTRM 480

BLAST of MS003405 vs. ExPASy TrEMBL
Match: A0A6J1JYB4 (nematode resistance protein-like HSPRO2 OS=Cucurbita maxima OX=3661 GN=LOC111489401 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.8e-241
Identity = 426/476 (89.50%), Postives = 452/476 (94.96%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-NTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLN 60
           MVDLGWKANMVSSDK  T+SPKLI    L+LSLP+SFRSS++STASPSSCS+Y+HYLRLN
Sbjct: 1   MVDLGWKANMVSSDKTTTKSPKLIPTANLSLSLPLSFRSSDISTASPSSCSSYDHYLRLN 60

Query: 61  DLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTT 120
           DLRKLWSS++FPEW SESVLKPGLQALEITFRFIS VLSDPRPY NR EWKRKLESLTT+
Sbjct: 61  DLRKLWSSKEFPEWTSESVLKPGLQALEITFRFISIVLSDPRPYVNRWEWKRKLESLTTS 120

Query: 121 QIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEAS 180
           QIQLIAMI EDDEED EARGR+PIVDLSSS+GVITREGSSAEVWK+RGEATVVSRTSE S
Sbjct: 121 QIQLIAMIFEDDEEDDEARGRVPIVDLSSSNGVITREGSSAEVWKIRGEATVVSRTSELS 180

Query: 181 LLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSL 240
           LLPRLASWQSSEDIAQMI Y VECEMRRCPYTLGLGEPNLAGKPN+DYD ICRPNELHSL
Sbjct: 181 LLPRLASWQSSEDIAQMIQYTVECEMRRCPYTLGLGEPNLAGKPNIDYDQICRPNELHSL 240

Query: 241 RKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERI 300
           RK+PYD QIENYENQTVYTTHQILESWI+AA ELLKRI ERIE K+FARAA DCYL+ERI
Sbjct: 241 RKSPYD-QIENYENQTVYTTHQILESWIYAAHELLKRIAERIEMKNFARAAKDCYLMERI 300

Query: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360
           WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF
Sbjct: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360

Query: 361 ILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMG 420
           IL+VEVDPMGGPRIQEAAM+LYSEKRE EKIHLLQALQ+IE+ IK+FFF+YKQVLV+VMG
Sbjct: 361 ILDVEVDPMGGPRIQEAAMRLYSEKREIEKIHLLQALQSIESAIKKFFFAYKQVLVMVMG 420

Query: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGD+H VFGSDRRTRMKL
Sbjct: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDMHSVFGSDRRTRMKL 475

BLAST of MS003405 vs. ExPASy TrEMBL
Match: A0A6J1FFX0 (nematode resistance protein-like HSPRO2 OS=Cucurbita moschata OX=3662 GN=LOC111445112 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.7e-240
Identity = 423/476 (88.87%), Postives = 451/476 (94.75%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK-NTRSPKLISNKQLNLSLPVSFRSSELSTASPSSCSAYEHYLRLN 60
           MVDLGWKANMVSSDK  T+SPKLI    L+LSLP+SFRSS++STASPSSCS+Y+HYLRLN
Sbjct: 1   MVDLGWKANMVSSDKTTTKSPKLIPTANLSLSLPLSFRSSDISTASPSSCSSYDHYLRLN 60

Query: 61  DLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTT 120
           DLRKLWSS++FPEW SESVLKPGLQALEITFRFIS VLSDPRPY NR EWKRKLESLTT+
Sbjct: 61  DLRKLWSSKEFPEWTSESVLKPGLQALEITFRFISIVLSDPRPYVNRWEWKRKLESLTTS 120

Query: 121 QIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEAS 180
           QIQLIAMI EDDEED EARGR+PIVDLSSS+GVITREGSSAEVWK+RGEATVVSRTSE S
Sbjct: 121 QIQLIAMIFEDDEEDDEARGRVPIVDLSSSNGVITREGSSAEVWKIRGEATVVSRTSELS 180

Query: 181 LLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSL 240
           LLPRLASWQSSEDIAQMI Y VECEMRRCPYTLGLGEPNL GKPN+DYD ICRPNELHSL
Sbjct: 181 LLPRLASWQSSEDIAQMIQYSVECEMRRCPYTLGLGEPNLVGKPNIDYDQICRPNELHSL 240

Query: 241 RKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERI 300
           RK+PYD QIENYENQTVYTTHQILESWI+AA ELLKRI ERIE K+FARAA DCYL+ERI
Sbjct: 241 RKSPYD-QIENYENQTVYTTHQILESWIYAAHELLKRIAERIEMKNFARAAKDCYLMERI 300

Query: 301 WKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360
           WK+LQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF
Sbjct: 301 WKVLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPF 360

Query: 361 ILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMG 420
           IL+VEVDPMGGPRIQEAAM+LYSEKRE EKIHLLQALQ+IE+ IK+FFF+YKQVLV+VMG
Sbjct: 361 ILDVEVDPMGGPRIQEAAMRLYSEKREIEKIHLLQALQSIESAIKKFFFAYKQVLVMVMG 420

Query: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTRMKL 476
           SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGD+H V+GSDRRTRMKL
Sbjct: 421 SLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDMHSVYGSDRRTRMKL 475

BLAST of MS003405 vs. ExPASy TrEMBL
Match: A0A5A7UGJ0 (Nematode resistance protein-like HSPRO2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43052G002350 PE=4 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.9e-240
Identity = 424/482 (87.97%), Postives = 457/482 (94.81%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDK------NTRSPKL--ISNKQLNLSLPVSFRSSELSTASPSSCSAY 60
           MVDLGWKANMVSSDK       T+SPK+  I+N +LNLSLPVSFRSSELSTASPSSCS+Y
Sbjct: 1   MVDLGWKANMVSSDKTTTTTTTTKSPKIIPIANNKLNLSLPVSFRSSELSTASPSSCSSY 60

Query: 61  EHYLRLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRK 120
           E YLRLNDLRKLWSS++FPEWR+ESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRK
Sbjct: 61  EQYLRLNDLRKLWSSKEFPEWRNESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRK 120

Query: 121 LESLTTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVV 180
           LESLTTTQIQLIAMICEDDEEDGE+RGR+PIVDLSSSDG+ TR+GSSAEVWKV GEATVV
Sbjct: 121 LESLTTTQIQLIAMICEDDEEDGESRGRVPIVDLSSSDGMFTRDGSSAEVWKVHGEATVV 180

Query: 181 SRTSEASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICR 240
           +RTSE+SLLPRLASWQSSEDIAQ+I Y VECEMRRCPYTLGLGEPNLAGKPNLDYDLIC+
Sbjct: 181 NRTSESSLLPRLASWQSSEDIAQLIQYSVECEMRRCPYTLGLGEPNLAGKPNLDYDLICK 240

Query: 241 PNELHSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASD 300
           PNELHSLRK+ Y +QIENYENQTVYTTHQILESWI++A ELLKRI ERIE+K+FA AASD
Sbjct: 241 PNELHSLRKSLY-EQIENYENQTVYTTHQILESWIYSAHELLKRIEERIEKKNFAGAASD 300

Query: 301 CYLLERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKD 360
           CY++ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKD
Sbjct: 301 CYMMERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKD 360

Query: 361 LKHKVPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQ 420
           LKHKVP IL+VEVDPMGGPRIQEAAMKLYSEK+E EKIHLLQA+QAIE+ IK+FFF+YKQ
Sbjct: 361 LKHKVPLILDVEVDPMGGPRIQEAAMKLYSEKQEPEKIHLLQAMQAIESAIKKFFFAYKQ 420

Query: 421 VLVIVMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFGSDRRTR 475
           VLV+VMGSLEAKGNRVVVSSNS+DSLSQIFLE TYFPSLDAAKTFLGD+H VFGSDRRTR
Sbjct: 421 VLVMVMGSLEAKGNRVVVSSNSEDSLSQIFLEPTYFPSLDAAKTFLGDLHSVFGSDRRTR 480

BLAST of MS003405 vs. TAIR 10
Match: AT2G40000.1 (ortholog of sugar beet HS1 PRO-1 2 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-142
Identity = 264/470 (56.17%), Postives = 327/470 (69.57%), Query Frame = 0

Query: 1   MVDLGWKANMVSSDKNTRSPKLISNKQLNLSLPVSFR----SSELSTASPSSCSAYEHYL 60
           MVD+ WK  MVSSD    SPKL S  +L++++P  F+    SS +S ++P+ CSAYE YL
Sbjct: 1   MVDMDWKRKMVSSDL-PNSPKLSS--KLHVTIPSPFKIVPVSSPISCSAPALCSAYELYL 60

Query: 61  RLNDLRKLWSSRDFPEWRSESVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESL 120
           RL +LRKLWSSRDFP+W SE +LKP LQALEI+FR +  V SD RPY N REW R+L+SL
Sbjct: 61  RLPELRKLWSSRDFPQWTSEPILKPALQALEISFRLVFAVCSDTRPYINHREWNRRLDSL 120

Query: 121 TTTQIQLIAMICEDDEEDGEARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTS 180
            T QIQL+A ICED+EE+G                             +  EA V    S
Sbjct: 121 ITKQIQLVAAICEDEEEEG-----------------------------ISAEAPVGGGRS 180

Query: 181 EASLLPRLASWQSSEDIAQMILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNEL 240
             SLLP+LA+W+ SE + + ILY ++ EM RC YTLGLGE N+AGKPNL YD ICRPNE+
Sbjct: 181 SLSLLPQLATWRRSEALGKKILYTIDNEMSRCKYTLGLGEQNIAGKPNLRYDAICRPNEI 240

Query: 241 HSLRKTPYDQQIENYENQTVYTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLL 300
           +SL+  PY   I+N+ENQT+Y  HQILESWI+A+  LL RI   IE + F +A++D YLL
Sbjct: 241 YSLKDNPYADHIDNHENQTLYIIHQILESWIYASGNLLNRIVSSIEEEKFGKASNDVYLL 300

Query: 301 ERIWKLLQEIEDLHLLMDPDDFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHK 360
           E+IWK+L EIEDLH+LMDP+DFLKLK QL IKS  +++AFCFRS  LVE+ K  KDL+ K
Sbjct: 301 EKIWKILAEIEDLHMLMDPEDFLKLKKQLQIKSTGKNDAFCFRSKGLVEMMKMSKDLRQK 360

Query: 361 VPFILEVEVDPMGGPRIQEAAMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVI 420
           VP +L VEVDP GGPR+QEAAMKLY+ K E +KIHLLQ +QA+EA  K FFF Y+Q++  
Sbjct: 361 VPAVLAVEVDPTGGPRLQEAAMKLYARKTECDKIHLLQGMQAVEAAAKSFFFGYRQLVAA 420

Query: 421 VMGSLEAKGNRVVVSSNSDDSLSQIFLESTYFPSLDAAKTFLGDIHGVFG 467
           +MGS E        S  S DSLSQIF+E TYFPSLDAAKTFLG+     G
Sbjct: 421 MMGSAEM---NATASQESCDSLSQIFMEPTYFPSLDAAKTFLGEFWSHLG 435

BLAST of MS003405 vs. TAIR 10
Match: AT3G55840.1 (Hs1pro-1 protein )

HSP 1 Score: 469.5 bits (1207), Expect = 3.1e-132
Identity = 249/444 (56.08%), Postives = 313/444 (70.50%), Query Frame = 0

Query: 21  KLISNKQLNLSLP----VSFRSSELSTASPSSCSAYEHYLRLNDLRKLWSSRDFPEWRSE 80
           K++S K L++++P    +S  SS +S++S ++CSAYE YLRL +LR LWSS  FP W SE
Sbjct: 9   KMVSPK-LHVTIPEPCKLSSVSSPISSSSSAACSAYELYLRLPELRNLWSSLYFPHWISE 68

Query: 81  SVLKPGLQALEITFRFISTVLSDPRPYANRREWKRKLESLTTTQIQLIAMICEDDEEDGE 140
            VLKP LQALEITFR I TV SD RPY NRREW R+L+SLTT+QI+++A IC D++   E
Sbjct: 69  PVLKPALQALEITFRLILTVASDTRPYINRREWIRRLDSLTTSQIKIVAAICGDEDNYEE 128

Query: 141 ARGRIPIVDLSSSDGVITREGSSAEVWKVRGEATVVSRTSEASLLPRLASWQSSEDIAQM 200
                P+     S+G           W            S  SLL  +A+ ++SE + Q 
Sbjct: 129 NVSAAPV-----SNG-----------W------------SSLSLLSEIATCRTSESVGQK 188

Query: 201 ILYCVECEMRRCPYTLGLGEPNLAGKPNLDYDLICRPNELHSLRKTPYDQQIENYENQTV 260
           IL  +E EMR C YTLGLGEPNLAGKP L YD +C P ELHSL+  PY   IEN ENQ +
Sbjct: 189 ILSTIENEMRWCKYTLGLGEPNLAGKPYLQYDAVCLPEELHSLKNNPYADHIENQENQML 248

Query: 261 YTTHQILESWIFAAQELLKRITERIERKDFARAASDCYLLERIWKLLQEIEDLHLLMDPD 320
           YT+HQILESWI+ +  LL RI  RIE   F +A+SD YLLERIWKLL EIEDLH+LMDP+
Sbjct: 249 YTSHQILESWIYVSVNLLYRIESRIEEGKFEKASSDVYLLERIWKLLSEIEDLHILMDPE 308

Query: 321 DFLKLKNQLAIKSLQESEAFCFRSTTLVEITKQCKDLKHKVPFILEVEVDPMGGPRIQEA 380
           DFLK+K QL IKS   ++AFCFRS  LVE+ K  K+L+ KVP +LEVEVDP GGPR+QEA
Sbjct: 309 DFLKVKKQLQIKSTFPNDAFCFRSKGLVEMAKMSKELRQKVPAVLEVEVDPTGGPRLQEA 368

Query: 381 AMKLYSEKREFEKIHLLQALQAIEAGIKRFFFSYKQVLVIVMGSLEAKGNRVVVSSNSDD 440
           AMKLYS K E+EKIHLLQ +QA+E+  KRFFF Y++++  ++G+ EA  NR V +  S D
Sbjct: 369 AMKLYSRKTEYEKIHLLQGMQAVESAAKRFFFGYQKLVAAMIGNAEANANRTVANHESYD 423

Query: 441 SLSQIFLESTYFPSLDAAKTFLGD 461
           SL+Q+F+E  Y+PSLDAAKTFLG+
Sbjct: 429 SLTQVFMEPPYYPSLDAAKTFLGE 423