MS003395 (gene) Bitter gourd (TR) v1

Overview


Name	MS003395
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Phosphoinositide phospholipase C
Location	scaffold234: 2593831 .. 2597670 (-)
RNA-Seq Expression	MS003395
Synteny	MS003395

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGGGGACACAACAGCTACAAGGTCCTCTGTTTCAGTAGGAAGTTCAAGATCACTGAGGCCCAACCGCCGCCCGACGTCAGAGATGCCTTCGCTACGTTTTCCGGTGGCGGGGACTACATGACCGCCGATCAACTGCTCCGTTTTCTGGTGGATCAACAAGGAGAGGAGGAAGTTACCCTATCTCAGGCCGAGCAGATTATCGAGCAGGTCTTGCAGAAGCGACGGAGCTCTGACAACAACCCCTCCCAACCCTCCGATCAGGGTTTCACCCTTGATGATTTCTTCCACTATCTCTTCATGGATAGTTTCAATGGTCCCATTAAATCTAAGGTTCGCTCGCTTTCCCTTTCCCATTTTGAACCCTTTTCGATTTGTTTCTTTGTTTAGCTGCAGATGTAGCCTTTCGTTTTGGATAGTGCCTTAAAGGGGAAAGAAGATGGGCATTATTTATAATTTTTTATAATAGTTTAAATTACAATGTGCGTCTATTTAATTCCTATGATTTAAAAAGTTTCTATCATCTTTGAATCTTTGAAGTTTCAATTTTGTGTATGATGATTCTTTGTTATCAACTCTGTTCGTTAATATTTTCGCGGGGTTGAATGTATTATTTAAGGATTATTAAAGCGGGAACAATTGTTCTCTATTTCTCTGTGCCATTACAGCTATCAAACCTGAACTTAAAACTAATTAATGTTATTTGTAGGTTATAAGGCTAAGGCAGTGGATTCTATAACTATGATTGAATTTGTGAAAGAACTTTATCATGTCAAGTTACTCAGAGAGAAACCAAACTCATAGCTGCAGTCTGACAATTAATTGAAGATGATTAGATTGTTAAACCATTAGATGCTTGTTGCGGTAATCCGAATCCAATGGAATATCATTTGCTTATAATCAATAATGCTTTTGATAACTGTACTTTTTACAGGTACATCATGATATGAATGCTCCATTGTCACATTATTTCATATATACAGGGCACAACTCTTACCTTACTGGGAATCAACTTAGTAGTGATTGTAGTGATGCTCCCATAATAAAGGCTTTGAAGAGAGGGCTGCGGGTAGTTGAGCTCGATTTATGGCCAAATTCAGCTAAAGATGACGTTCATGTTCTTCATGGAAGGTACTAGCTGATCGTTCAATATCTTCATGTCATCAAATGAAAATATAAACTGATGTTAAACTTTAAAAGAGGAAATCACTGTACAGAACCATTCTGTGGATGACTGTAGGACTTTAACCAGCCCTGTGACTCTCTTGAAGTGTTTGAAGTCTATAAACAAGCATGCTTTTGAGACATCTCCATATCCTGTCATTATTACTCTTGAAGATCATCTTACTCCTGGTCTTCAAGCTAAAGTTGCGGAGGTTAAAGATTATTTTCCTACTCGAAATAATTTCTCTCTATAAATATTCTGCTTGAATATGATTAATATGTTGTGTTTCCTTGCAGATGGTAACTGGAATATTTGGAGATACACTGTTCTACCCTCAGGAAGATAGCTTGTCAGAATTCCCTTCTCCAGAATCCCTGAAATATAGAATCATTATTTCAACAAAGCCACCAAAAGAGTACCTAGAATCTAAGAAGAAAAAAGACAAGGTTTTATGTGATGGAAAAGAGTCATCAGAAGAGGAAACATCAGGAAAGGAGACTCCAGACCTTATGACTGTTTTTGAAGTTGAGGAACTGGTGGGTGAAAAATGAAATTTTGGATTATCATTTCTTATTTTGATGCAGGGTGGAGCAAAGAAAGAAGGAGAGAGAGTTTTTCCTAATTTGAAAACTATGTATAAAATACTTAGGCTTTGTAGTTTTTCTCTATTTTGCCCCATTTCAAATTTTCAGAAATTAAAGATTCTATATATATATATACAGAGTTAGCATTTAAAGTTTTTCCCCACTCTAAGAAATTATCTTTCTACCGGTGTTTTAAAGTATATACAACAGCTTGATTGGCTCTTTTAATTGGTGACAGAGTGAAAGTGACCCAGATGATGAAGAAACCAGTGCAGTGGAGAGCAAACCGTTACAGCAAGGTGCACCTGAATACAAACGTCTAATCACAATTCATGCTGGGAAACCAAAAGGTGGCTGCTTAAAGGATGCATTGAAGGTACATGGTGACAAAGTTAGGCGCCTTAGCTTGAGTGAGCAGGCACTTCAAAAGGCCGTAGCCTCTAATGGAACTGATGTTGTAAGGTAATTCTTCAACTTATAATGTGGAAGAGATTCGTTTCTTCATAGCAAACTTCGTTTTTGGCTGGTGATTGATTCTGATACTTGCTGTGATCTTTAGGTTCACACAGACGAATGTTTTGAGAGTTTACCCCAAAGGGACACGGGTCACGTCCTCAAATTTCAGGCCAAATATTGGGTGGATCCATGGAGCTCAGATGGTGGCTTTAAATATGCAGGTGCTTGTTGGAACCTTCATCACGTCCTCCACTTTTTTTACCCTCTTGCCCCTTTACATTCTTTTGCATGACAACTTAAAATATTATGCTGCATGAATAACGGTGGTGGTATGGTATCGTTTACTCGAAGGGGAGAACGGTCCTCTGAATGAGTGGGTAAAATGTTGAATTAAGCTATACAATTGCATACGAATAATGAAATAACGAGGCTATGCAATGCTATTGATGATTTTTTCTACATTACCATGTAATGGTGTTTTCAAATGTCTTACAATTCTATGTTTTCACAACAGAAATATAATAAATTTTTGCTTTCATTCACTATTTGGAGGGATCCAAATGAAGCACCTGGTTTTTTTTCTACAACAGGGCTATGGAAGATCATTGTGGCTGATGCATGGGATGTTTAGAGCCAACGGAGGGTGTGGTTACTTGAAAAAACCCGACTTCCTCTTGCAAAAAGGCCCACAAAATGAGGTCTTTGATCCCAAGAAAGCTTTACCAGTGAAGGAAACGTTAAAAGTAAGCCAATCGTGTTGGGTTATAAATTGTAATTAAATTTGGTTCTTTTGGAATCGGTGTTAACATTCATGTTATTCAAATACAGGTAACCGTATATTTGGGGGATGGATGGAGCTCTGACTTTAGCCAAACGCATTTTGACAACTACTCCCCACCAGACTTCTACACAAAGGTTAGTGGTGACCATTCAGTCCTCCTATCAGGAATACTTTTTCATCATCTTCTGCTGGATTTGATATTGCTAATTCTGGAATAGCATGGAATTTGGCTAAGGTTTATATGTGAATATAACTAGAAAAGCAGATGATGTCAAATATATCTCCATGAGAATTTATCAAGCCTGGGGTTTGTCAGACAAACCAATAAGCTTACTAAACTTGTACCCCAAACACAAGCTTCCTAAAACCTGGCTTAAGATACCGAACCTGCATCCCAAATGGGTCTTAATCTTAACTTAAAATTTTTGTTGTCTCCGAGGCATCCTTCTGAGTGGGGCGTGGGCCATTTGCATGATATAAAGAGTGGGGCGTAGCCTGTTTATTTAAAAATGAAAGAAAGAAGTTATAAACTAAAGCTATAAATATGGGATGAATGGCAGATTCGTATTGTTGGGGTGCCAGCTGATGCAGCGAAGAAGAAGACAAAGACTATTGAGAACAATTGGGTGCCTATTTGGAACGAAGAGTTCAAGTTCCCATTGACAGTTCCAGAGCTTGCTTTGATGCAGCTTGAAGTTCGAGATTTTGACAGGTCGGAGAAAGATGATTTTGGCGGGCAGACATGTTTGCCCATCACAGAGCTCAAGCCTGGGATTCGGGCGGTCCCTCTCTACGACAAGAAGGGAGAGAAATTAAAGTCCGTAAGGCTTCTCATGCGCTTCGAGTTTGTA

mRNA sequence

ATGAAGGGGGGACACAACAGCTACAAGGTCCTCTGTTTCAGTAGGAAGTTCAAGATCACTGAGGCCCAACCGCCGCCCGACGTCAGAGATGCCTTCGCTACGTTTTCCGGTGGCGGGGACTACATGACCGCCGATCAACTGCTCCGTTTTCTGGTGGATCAACAAGGAGAGGAGGAAGTTACCCTATCTCAGGCCGAGCAGATTATCGAGCAGGTCTTGCAGAAGCGACGGAGCTCTGACAACAACCCCTCCCAACCCTCCGATCAGGGTTTCACCCTTGATGATTTCTTCCACTATCTCTTCATGGATAGTTTCAATGGTCCCATTAAATCTAAGGTACATCATGATATGAATGCTCCATTGTCACATTATTTCATATATACAGGGCACAACTCTTACCTTACTGGGAATCAACTTAGTAGTGATTGTAGTGATGCTCCCATAATAAAGGCTTTGAAGAGAGGGCTGCGGGTAGTTGAGCTCGATTTATGGCCAAATTCAGCTAAAGATGACGTTCATGTTCTTCATGGAAGGACTTTAACCAGCCCTGTGACTCTCTTGAAGTGTTTGAAGTCTATAAACAAGCATGCTTTTGAGACATCTCCATATCCTGTCATTATTACTCTTGAAGATCATCTTACTCCTGGTCTTCAAGCTAAAGTTGCGGAGATGGTAACTGGAATATTTGGAGATACACTGTTCTACCCTCAGGAAGATAGCTTGTCAGAATTCCCTTCTCCAGAATCCCTGAAATATAGAATCATTATTTCAACAAAGCCACCAAAAGAGTACCTAGAATCTAAGAAGAAAAAAGACAAGGTTTTATGTGATGGAAAAGAGTCATCAGAAGAGGAAACATCAGGAAAGGAGACTCCAGACCTTATGACTGTTTTTGAAGTTGAGGAACTGAGTGAAAGTGACCCAGATGATGAAGAAACCAGTGCAGTGGAGAGCAAACCGTTACAGCAAGGTGCACCTGAATACAAACGTCTAATCACAATTCATGCTGGGAAACCAAAAGGTGGCTGCTTAAAGGATGCATTGAAGGTACATGGTGACAAAGTTAGGCGCCTTAGCTTGAGTGAGCAGGCACTTCAAAAGGCCGTAGCCTCTAATGGAACTGATGTTGTAAGGTTCACACAGACGAATGTTTTGAGAGTTTACCCCAAAGGGACACGGGTCACGTCCTCAAATTTCAGGCCAAATATTGGGTGGATCCATGGAGCTCAGATGGTGGCTTTAAATATGCAGGGCTATGGAAGATCATTGTGGCTGATGCATGGGATGTTTAGAGCCAACGGAGGGTGTGGTTACTTGAAAAAACCCGACTTCCTCTTGCAAAAAGGCCCACAAAATGAGGTCTTTGATCCCAAGAAAGCTTTACCAGTGAAGGAAACGTTAAAAGTAACCGTATATTTGGGGGATGGATGGAGCTCTGACTTTAGCCAAACGCATTTTGACAACTACTCCCCACCAGACTTCTACACAAAGATTCGTATTGTTGGGGTGCCAGCTGATGCAGCGAAGAAGAAGACAAAGACTATTGAGAACAATTGGGTGCCTATTTGGAACGAAGAGTTCAAGTTCCCATTGACAGTTCCAGAGCTTGCTTTGATGCAGCTTGAAGTTCGAGATTTTGACAGGTCGGAGAAAGATGATTTTGGCGGGCAGACATGTTTGCCCATCACAGAGCTCAAGCCTGGGATTCGGGCGGTCCCTCTCTACGACAAGAAGGGAGAGAAATTAAAGTCCGTAAGGCTTCTCATGCGCTTCGAGTTTGTA

Coding sequence (CDS)

ATGAAGGGGGGACACAACAGCTACAAGGTCCTCTGTTTCAGTAGGAAGTTCAAGATCACTGAGGCCCAACCGCCGCCCGACGTCAGAGATGCCTTCGCTACGTTTTCCGGTGGCGGGGACTACATGACCGCCGATCAACTGCTCCGTTTTCTGGTGGATCAACAAGGAGAGGAGGAAGTTACCCTATCTCAGGCCGAGCAGATTATCGAGCAGGTCTTGCAGAAGCGACGGAGCTCTGACAACAACCCCTCCCAACCCTCCGATCAGGGTTTCACCCTTGATGATTTCTTCCACTATCTCTTCATGGATAGTTTCAATGGTCCCATTAAATCTAAGGTACATCATGATATGAATGCTCCATTGTCACATTATTTCATATATACAGGGCACAACTCTTACCTTACTGGGAATCAACTTAGTAGTGATTGTAGTGATGCTCCCATAATAAAGGCTTTGAAGAGAGGGCTGCGGGTAGTTGAGCTCGATTTATGGCCAAATTCAGCTAAAGATGACGTTCATGTTCTTCATGGAAGGACTTTAACCAGCCCTGTGACTCTCTTGAAGTGTTTGAAGTCTATAAACAAGCATGCTTTTGAGACATCTCCATATCCTGTCATTATTACTCTTGAAGATCATCTTACTCCTGGTCTTCAAGCTAAAGTTGCGGAGATGGTAACTGGAATATTTGGAGATACACTGTTCTACCCTCAGGAAGATAGCTTGTCAGAATTCCCTTCTCCAGAATCCCTGAAATATAGAATCATTATTTCAACAAAGCCACCAAAAGAGTACCTAGAATCTAAGAAGAAAAAAGACAAGGTTTTATGTGATGGAAAAGAGTCATCAGAAGAGGAAACATCAGGAAAGGAGACTCCAGACCTTATGACTGTTTTTGAAGTTGAGGAACTGAGTGAAAGTGACCCAGATGATGAAGAAACCAGTGCAGTGGAGAGCAAACCGTTACAGCAAGGTGCACCTGAATACAAACGTCTAATCACAATTCATGCTGGGAAACCAAAAGGTGGCTGCTTAAAGGATGCATTGAAGGTACATGGTGACAAAGTTAGGCGCCTTAGCTTGAGTGAGCAGGCACTTCAAAAGGCCGTAGCCTCTAATGGAACTGATGTTGTAAGGTTCACACAGACGAATGTTTTGAGAGTTTACCCCAAAGGGACACGGGTCACGTCCTCAAATTTCAGGCCAAATATTGGGTGGATCCATGGAGCTCAGATGGTGGCTTTAAATATGCAGGGCTATGGAAGATCATTGTGGCTGATGCATGGGATGTTTAGAGCCAACGGAGGGTGTGGTTACTTGAAAAAACCCGACTTCCTCTTGCAAAAAGGCCCACAAAATGAGGTCTTTGATCCCAAGAAAGCTTTACCAGTGAAGGAAACGTTAAAAGTAACCGTATATTTGGGGGATGGATGGAGCTCTGACTTTAGCCAAACGCATTTTGACAACTACTCCCCACCAGACTTCTACACAAAGATTCGTATTGTTGGGGTGCCAGCTGATGCAGCGAAGAAGAAGACAAAGACTATTGAGAACAATTGGGTGCCTATTTGGAACGAAGAGTTCAAGTTCCCATTGACAGTTCCAGAGCTTGCTTTGATGCAGCTTGAAGTTCGAGATTTTGACAGGTCGGAGAAAGATGATTTTGGCGGGCAGACATGTTTGCCCATCACAGAGCTCAAGCCTGGGATTCGGGCGGTCCCTCTCTACGACAAGAAGGGAGAGAAATTAAAGTCCGTAAGGCTTCTCATGCGCTTCGAGTTTGTA

Protein sequence

MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAPLSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDSLSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSLSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSDFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV

Homology

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BLAST of MS003395 vs. NCBI nr
Match: XP_022134059.1 (phosphoinositide phospholipase C 6-like [Momordica charantia])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV
Sbjct: 55  MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 114

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP
Sbjct: 115 TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 174

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 175 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 234

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS
Sbjct: 235 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 294

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV
Sbjct: 295 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 354

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL
Sbjct: 355 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 414

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG
Sbjct: 415 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 474

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD
Sbjct: 475 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 534

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ
Sbjct: 535 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 594

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV
Sbjct: 595 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 648

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BLAST of MS003395 vs. NCBI nr
Match: XP_038884114.1 (phosphoinositide phospholipase C 6 [Benincasa hispida])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.8e-309
Identity = 523/595 (87.90%), Postives = 557/595 (93.61%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG ++YKVLCF+RKFK TEA+PPPDVR+ FA FSGGGDYM+ADQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSNYKVLCFNRKFKTTEAEPPPDVRNLFAAFSGGGDYMSADQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           T S+AEQI+EQVLQKRRSSD NPS  S +GF+LDDFFHYLFMD FNGPIK++VHHDMNAP
Sbjct: 61  TPSEAEQILEQVLQKRRSSD-NPSGDSPRGFSLDDFFHYLFMDYFNGPIKTQVHHDMNAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSIN+HAFE SPYP+IITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ +S
Sbjct: 181 TSPVTLLKCLKSINEHAFEKSPYPIIITLEDHLTPTLQAKVAEMVTGIFGDTLFYPQAES 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLESK KK K+L DGKESSEEETSG+ETPDL T F+ 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLESKHKKGKILSDGKESSEEETSGRETPDLATDFDA 300

Query: 301 EELSESDPDDEETSAV-ESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLS 360
           EE SESDPDD++ S V E K  QQGAPEYKRLITIHAGKPKGGCLKDAL V GDKVRRLS
Sbjct: 301 EEQSESDPDDDDESIVGELKTCQQGAPEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLS 360

Query: 361 LSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGY 420
           LSEQ L+KA+ SNG+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW+HGAQMVALNMQGY
Sbjct: 361 LSEQGLEKAIVSNGSDVVRFTQKNILRVYPKGTRVTSSNFRPNIGWMHGAQMVALNMQGY 420

Query: 421 GRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSS 480
           GRSLWLMHGMFRANGGCGYLKKPDFLL K PQNEVFDPK+ LPVKETLKVTVYLGDGWSS
Sbjct: 421 GRSLWLMHGMFRANGGCGYLKKPDFLLHKSPQNEVFDPKRPLPVKETLKVTVYLGDGWSS 480

Query: 481 DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALM 540
           DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTK IEN+WVPIWNEEFKFPLTVPELAL+
Sbjct: 481 DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKVIENDWVPIWNEEFKFPLTVPELALL 540

Query: 541 QLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           Q+EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKKGEKLKSVRLLMRF+F+
Sbjct: 541 QIEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKKGEKLKSVRLLMRFQFL 594

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BLAST of MS003395 vs. NCBI nr
Match: XP_008441466.1 (PREDICTED: phosphoinositide phospholipase C 6 [Cucumis melo])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 513/594 (86.36%), Postives = 541/594 (91.08%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ FA+FSGG DYM+ADQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKTTEAEPPPDVRNLFASFSGGRDYMSADQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
             S+AEQI++QVLQ+RRSSD +PS    QGFTLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  APSEAEQILQQVLQRRRSSD-SPSDHLVQGFTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPNLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGMETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESDPDD+E+   E K  QQGAPEYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDPDDDESIVGELKTFQQGAPEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV SNG+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSNGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDP + LPVKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPSRPLPVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK IENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVIENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           +EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKKGEKLKS RLLMRF+F+
Sbjct: 541 MEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKKGEKLKSARLLMRFQFL 585

[Close]

BLAST of MS003395 vs. NCBI nr
Match: XP_004138421.1 (phosphoinositide phospholipase C 6 [Cucumis sativus] >KGN45789.1 hypothetical protein Csa_005440 [Cucumis sativus])

HSP 1 Score: 1023.5 bits (2645), Expect = 7.5e-295
Identity = 507/594 (85.35%), Postives = 536/594 (90.24%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ F++FS G DYM+ DQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKSTEAEPPPDVRNLFSSFSDGRDYMSPDQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           T S+A+QI++QVL KRRSSD NPS    QG TLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  TPSEADQILQQVLHKRRSSD-NPSDDLVQGLTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPSLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGLETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESD DD+E+   E K  QQGA EYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDQDDDESIVGELKTFQQGALEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV S+G+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSSGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDPK+ L VKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPKRPLTVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK +ENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVVENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           +EVRDFDRSEKDDFGGQTCLPI+ELKPGIRAVPLYDKKGEK KSVRLLMRF+F+
Sbjct: 541 IEVRDFDRSEKDDFGGQTCLPISELKPGIRAVPLYDKKGEKFKSVRLLMRFQFL 585

[Close]

BLAST of MS003395 vs. NCBI nr
Match: KAA0055041.1 (phosphoinositide phospholipase C 6 [Cucumis melo var. makuwa] >TYK24436.1 phosphoinositide phospholipase C 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.9e-292
Identity = 500/578 (86.51%), Postives = 526/578 (91.00%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ FA+FSGG DYM+ADQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKTTEAEPPPDVRNLFASFSGGRDYMSADQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
             S+AEQI++QVLQ+RRSSD +PS    QGFTLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  APSEAEQILQQVLQRRRSSD-SPSDHLVQGFTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPNLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGMETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESDPDD+E+   E K  QQGAPEYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDPDDDESIVGELKTFQQGAPEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV SNG+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSNGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDP + LPVKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPSRPLPVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK IENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVIENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKK 579
           +EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKK
Sbjct: 541 MEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKK 569

[Close]

BLAST of MS003395 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 755.4 bits (1949), Expect = 5.0e-217
Identity = 384/611 (62.85%), Postives = 462/611 (75.61%), Query Frame = 0


Query: 3   GGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGG----------------DYMTADQ 62
           G +N      ++RKFKI E  P  DVRDAF  F+ GG                  M A+Q
Sbjct: 15  GSYNYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQ 74

Query: 63  LLRFLVDQQGEEEVTLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFN 122
           L  FL D    E  T+++A+++I++V+++R    ++ ++ +  G  LDDFF++LF D  N
Sbjct: 75  LCSFLDDH--GESTTVAEAQRLIDEVIRRR----HHVTRFTRHGLDLDDFFNFLFYDDLN 134

Query: 123 GPIKSKVHHDMNAPLSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPN 182
            PI   VH DM APLSHYFIYTGHNSYLTGNQLSSDCS+ P+IKAL+RG+RV+ELDLWPN
Sbjct: 135 PPITPHVHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPN 194

Query: 183 SAKDDVHVLHGRTLTSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVT 242
           S   D++VLHGRTLT+PV L+KCLKSI  +AF +SPYPVIITLEDHLTP LQAKVAEM T
Sbjct: 195 STGTDINVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMAT 254

Query: 243 GIFGDTLFYPQEDSLSEFPSPESLKYRIIISTKPPKEYLESK----KKKDKVLCDGKESS 302
            IFG  L+YP+ DSL EFPSP SL +RIIISTKPPKEYLES+    K+KD  +    E  
Sbjct: 255 QIFGQMLYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDE 314

Query: 303 EEETSGKETPDLMTVFEVEELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGG 362
              T   +T + M +F+ +  S+SD D E+  A E +      P YKRLITIHAGKPK G
Sbjct: 315 TPRTEEIQTLESM-LFDQDFESKSDSDQEDEEASEDQ-----KPAYKRLITIHAGKPK-G 374

Query: 363 CLKDALKVHGDKVRRLSLSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPN 422
            +K+ +KV  DKVRRLSLSEQ L +  +SN  DVVRFTQ N+LR+YPKGTR  SSN++P 
Sbjct: 375 TVKEEMKVVVDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPL 434

Query: 423 IGWIHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALP 482
           IGW HGAQM+A NMQGYG+SLWLMHGMFRANGGCGY+KKP+FL++KG  +EVFDP+K LP
Sbjct: 435 IGWTHGAQMIAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLP 494

Query: 483 VKETLKVTVYLGDGWSSDFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPI 542
           VKETLKV VY+GDGW  DFS THFD YSPPDFYTK+ IVGVPAD AKKKTK IE+NW PI
Sbjct: 495 VKETLKVKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPI 554

Query: 543 WNEEFKFPLTVPELALMQLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKL 594
           W+EEF FPLTVPELAL+++EVR++D SEKDDFGGQTCLP+ EL+PGIR+VPLYDKKGEK+
Sbjct: 555 WDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKM 612

[Close]

BLAST of MS003395 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 7.2e-200
Identity = 353/592 (59.63%), Postives = 434/592 (73.31%), Query Frame = 0


Query: 7   SYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           +YKV  CF R+F+ T ++ P +++  F  +S  G  MT D L RFL+D Q +++ T   A
Sbjct: 5   TYKVCFCFRRRFRYTASEAPREIKTIFEKYSENG-VMTVDHLHRFLIDVQKQDKATREDA 64

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIK-SKVHHDMNAPLSHY 126
           + II          ++  S     G  LD FF YLF D+ N P+   KVHHDM+AP+SHY
Sbjct: 65  QSII----------NSASSLLHRNGLHLDAFFKYLFGDN-NPPLALHKVHHDMDAPISHY 124

Query: 127 FIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPV 186
           FI+TGHNSYLTGNQLSSDCS+ PII ALK+G+RV+ELD+WPNS KDD+ VLHG TLT+PV
Sbjct: 125 FIFTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPV 184

Query: 187 TLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQ-EDSLSE 246
            L+KCLK+I  HAF+ S YPV++TLEDHLTP LQ+KVAEMVT IFG+ LF P   +SL E
Sbjct: 185 GLIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKE 244

Query: 247 FPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEEL 306
           FPSP SLK RIIISTKPPKEY E K    +V+  GK+  +EE  G+E P  +   + E  
Sbjct: 245 FPSPNSLKRRIIISTKPPKEYKEGKDV--EVVQKGKDLGDEEVWGREVPSFIQRNKSEAK 304

Query: 307 SESD-PDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSLSE 366
            + D  DD++    E K      P+YK LI IHAGKPKGG + + LKV  DKVRRLSLSE
Sbjct: 305 DDLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGG-ITECLKVDPDKVRRLSLSE 364

Query: 367 QALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYGRS 426
           + L+KA       +VRFTQ N+LR+YPKGTRVTSSN+ P +GW HGAQMVA NMQGYGRS
Sbjct: 365 EQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRS 424

Query: 427 LWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSDFS 486
           LWLM GMFRANGGCGY+KKPD LL+ G  +++FDPK  LPVK TL+VTVY+G+GW  DF 
Sbjct: 425 LWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFR 484

Query: 487 QTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQLE 546
            THFD YSPPDFYT++ I GVP D   KKTKT+E+NW+P W+E F+FPLTVPELAL++LE
Sbjct: 485 HTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLE 544

Query: 547 VRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           V ++D SEKDDFGGQTCLP+ EL  GIRA PL+ +KGEK KSV+LL++ EFV
Sbjct: 545 VHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

[Close]

BLAST of MS003395 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 673.7 bits (1737), Expect = 1.9e-192
Identity = 343/595 (57.65%), Postives = 438/595 (73.61%), Query Frame = 0


Query: 7   SYK-VLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           SYK  L F+RKF++TE+ P  DVRD F  ++ G  +M+ +QL + + ++ GE E +L +A
Sbjct: 9   SYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLEEA 68

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAPLSHYF 126
           E+I+++VL+++    ++ ++ + +  TLDDF +YLF    N PI  +VH +M+APLSHYF
Sbjct: 69  ERIVDEVLRRK----HHIAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYF 128

Query: 127 IYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPVT 186
           I+TGHNSYLTGNQLSS+CS+ PI  AL+RG+RVVELDLWP    DDV V HGRTLT  V 
Sbjct: 129 IFTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRGT-DDVCVKHGRTLTKEVK 188

Query: 187 LLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDSLSEFP 246
           L KCL+SI  +AF  S YPVIITLEDHLTP LQ KVA+M+T  FGD L+Y       EFP
Sbjct: 189 LGKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFP 248

Query: 247 SPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLM-TVFEVEELS 306
           SPE LK +I+ISTKPPKEYLE+   K+K   +  + S+E+  GKE  DL+ T  ++++++
Sbjct: 249 SPEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDEDVWGKEPEDLISTQSDLDKVT 308

Query: 307 ES----DPDDEETSAVES-KPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLS 366
            S    + DDEE  + ES    Q  APEYKRLI IHAGKPKGG L+ ALKV  +K+RRLS
Sbjct: 309 SSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGG-LRMALKVDPNKIRRLS 368

Query: 367 LSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGY 426
           LSEQ L+KAVAS G DV+RFTQ N LR+YPKGTR  SSN++P IGW+ GAQM+A NMQGY
Sbjct: 369 LSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGY 428

Query: 427 GRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSS 486
           GR+LWLM GMFRANGGCGY+KKPDFL+   P  + F PK     K+TLKV V +GDGW  
Sbjct: 429 GRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLL 488

Query: 487 DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALM 546
           DF +THFD+YSPPDF+ ++ I G P D   +KTK   + W PIWN+EF FPL VPELAL+
Sbjct: 489 DFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALL 548

Query: 547 QLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           ++EV + D +EKDDFGGQTCLP++E++ GIRAVPL+++KG K  S RLLMRFEFV
Sbjct: 549 RVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEFV 597

[Close]

BLAST of MS003395 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.8e-188
Identity = 338/598 (56.52%), Postives = 428/598 (71.57%), Query Frame = 0


Query: 7   SYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           +YKV  CF R+++ T +  P +++  F  +S  G  MT D LLRFL+D Q +++ T  +A
Sbjct: 5   TYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKG-LMTTDLLLRFLIDVQKQDKATKEEA 64

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKS-KVHHDMNAPLSHY 126
           + I+          + + S     G  LD FF YLF  + N P+ S +VH DM+APLSHY
Sbjct: 65  QDIV----------NASSSLLHRNGLHLDAFFKYLFAVT-NSPLSSLEVHQDMDAPLSHY 124

Query: 127 FIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPV 186
           FIYTGHNSYLTGNQLSSDCS+ PII+ALK+G+RV+ELD+WPNS +D + VLHGRTLTSPV
Sbjct: 125 FIYTGHNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPV 184

Query: 187 TLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQE-DSLSE 246
            L+KCL++I +HAF+ S YPV++TLEDHLTP LQAKVAEMVT IFG+ LF P   + L E
Sbjct: 185 ELIKCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKE 244

Query: 247 FPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEEL 306
           FPSP  LK RI+ISTKPPKEY       D ++  G++  ++E  G+E P  +      + 
Sbjct: 245 FPSPAFLKKRIMISTKPPKEY--KAATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDK 304

Query: 307 SES-------DPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVR 366
           ++S       D DD++    + K  +   PEYK LI I AGKPKGG + + LKV  DKVR
Sbjct: 305 NDSNGDDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGG-ITECLKVDPDKVR 364

Query: 367 RLSLSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNM 426
           RLSLSE+ L+KA       +VRFTQ N+LRVYPKGTR+TSSN+ P I W HGAQMVA NM
Sbjct: 365 RLSLSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNM 424

Query: 427 QGYGRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDG 486
           QG GRSLW+M GMFR NGGCGY+KKPD LL+    N VFDP+  LPVK TL+VT+Y+G+G
Sbjct: 425 QGLGRSLWVMQGMFRGNGGCGYIKKPDLLLK---SNAVFDPEATLPVKTTLRVTIYMGEG 484

Query: 487 WSSDFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPEL 546
           W  DF  THFD YSPPDFYT++ I GVPAD   KKTKT+E+NW+P W+E F+FPLTVPEL
Sbjct: 485 WYYDFPHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPEL 544

Query: 547 ALMQLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           AL+++EV ++D SEKDDFGGQ CLP+ EL+ GIRAVPL ++ G K +SV+LL+R EFV
Sbjct: 545 ALLRIEVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRLEFV 584

[Close]

BLAST of MS003395 vs. ExPASy Swiss-Prot
Match: Q944C2 (Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 SV=2)

HSP 1 Score: 649.0 bits (1673), Expect = 5.0e-185
Identity = 330/595 (55.46%), Postives = 417/595 (70.08%), Query Frame = 0


Query: 1   MKGGHNSYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEE 60
           MK    SYK+ LC S K ++    PP DV  AF  ++ G  +MTA+QL RFLV+ Q E E
Sbjct: 1   MKRDMGSYKMGLCCSDKLRMNRGAPPQDVVTAFVEYTEGRSHMTAEQLCRFLVEVQDETE 60

Query: 61  VTLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNA 120
           V +S AE+IIE++  +R    ++ ++       LDDFF +LF D  N PI SKVH DM +
Sbjct: 61  VLVSDAEKIIERITCER----HHITKFLRHTLNLDDFFSFLFSDDLNHPIDSKVHQDMAS 120

Query: 121 PLSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRT 180
           PLSHYFIYT HNSYLTGNQ++S+CSD P+IKALKRG+R +ELD+WPNS KDD+ VLHG  
Sbjct: 121 PLSHYFIYTSHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWA 180

Query: 181 LTSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQED 240
            T PV L+KCL+SI +HAF  S YPVI+TLEDHLTP LQAK AEM+  IF D +++P+  
Sbjct: 181 WTPPVELVKCLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAG 240

Query: 241 SLSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFE 300
            L EFPSPE LKY+I+ISTKPPK  L    +KDK        SE + SGK + D+    E
Sbjct: 241 GLKEFPSPEDLKYKIVISTKPPKGSL----RKDK-------DSESDASGKASSDVSADDE 300

Query: 301 VEELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLS 360
             E   S+  +EE    + +        Y RLITI +G  K G LK+AL +    VRRLS
Sbjct: 301 KTEEETSEAKNEE-DGFDQESSNLDFLTYSRLITIPSGNAKNG-LKEALTIDNGGVRRLS 360

Query: 361 LSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGY 420
           L EQ  +KA    GT+V++FTQ N+LR+YPK TRV SSN+RP  GW++GAQMVA NMQGY
Sbjct: 361 LREQKFKKATEMYGTEVIKFTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQGY 420

Query: 421 GRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSS 480
           GR+LW+MHGMFR NGGCGY+KKPDF++      EVF+PK  LP+K+TLKV VY+G GW S
Sbjct: 421 GRALWMMHGMFRGNGGCGYVKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWDS 480

Query: 481 DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALM 540
            F +T F+ +S P+FYT++ I GV  D   KKTK  +  W P WNEEF+F LTVPELAL+
Sbjct: 481 GFQRTCFNTWSSPNFYTRVGITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELALL 540

Query: 541 QLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           ++EV D++  EKDDF GQTCLP++EL+ GIR+VPLYD+KGE+L SV LLMRF F+
Sbjct: 541 RIEVHDYNMPEKDDFSGQTCLPVSELRQGIRSVPLYDRKGERLVSVTLLMRFHFL 578

[Close]

BLAST of MS003395 vs. ExPASy TrEMBL
Match: A0A6J1BXQ4 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111006425 PE=4 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV
Sbjct: 55  MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 114

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP
Sbjct: 115 TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 174

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 175 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 234

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS
Sbjct: 235 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 294

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV
Sbjct: 295 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 354

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL
Sbjct: 355 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 414

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG
Sbjct: 415 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 474

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD
Sbjct: 475 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 534

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ
Sbjct: 535 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 594

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV
Sbjct: 595 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 648

[Close]

BLAST of MS003395 vs. ExPASy TrEMBL
Match: A0A1S3B496 (Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103485573 PE=4 SV=1)

HSP 1 Score: 1039.3 bits (2686), Expect = 6.4e-300
Identity = 513/594 (86.36%), Postives = 541/594 (91.08%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ FA+FSGG DYM+ADQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKTTEAEPPPDVRNLFASFSGGRDYMSADQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
             S+AEQI++QVLQ+RRSSD +PS    QGFTLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  APSEAEQILQQVLQRRRSSD-SPSDHLVQGFTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPNLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGMETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESDPDD+E+   E K  QQGAPEYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDPDDDESIVGELKTFQQGAPEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV SNG+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSNGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDP + LPVKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPSRPLPVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK IENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVIENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           +EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKKGEKLKS RLLMRF+F+
Sbjct: 541 MEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKKGEKLKSARLLMRFQFL 585

[Close]

BLAST of MS003395 vs. ExPASy TrEMBL
Match: A0A0A0KAC7 (Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_6G011650 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.6e-295
Identity = 507/594 (85.35%), Postives = 536/594 (90.24%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ F++FS G DYM+ DQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKSTEAEPPPDVRNLFSSFSDGRDYMSPDQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           T S+A+QI++QVL KRRSSD NPS    QG TLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  TPSEADQILQQVLHKRRSSD-NPSDDLVQGLTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPSLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGLETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESD DD+E+   E K  QQGA EYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDQDDDESIVGELKTFQQGALEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV S+G+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSSGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDPK+ L VKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPKRPLTVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK +ENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVVENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           +EVRDFDRSEKDDFGGQTCLPI+ELKPGIRAVPLYDKKGEK KSVRLLMRF+F+
Sbjct: 541 IEVRDFDRSEKDDFGGQTCLPISELKPGIRAVPLYDKKGEKFKSVRLLMRFQFL 585

[Close]

BLAST of MS003395 vs. ExPASy TrEMBL
Match: A0A5A7UN86 (Phosphoinositide phospholipase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G002370 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.9e-292
Identity = 500/578 (86.51%), Postives = 526/578 (91.00%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG +SYKVLCFSRKFK TEA+PPPDVR+ FA+FSGG DYM+ADQ LRFLVDQQG+ E 
Sbjct: 1   MKGGCSSYKVLCFSRKFKTTEAEPPPDVRNLFASFSGGRDYMSADQFLRFLVDQQGDVEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
             S+AEQI++QVLQ+RRSSD +PS    QGFTLDDFFHYLFMD FNGPIK++VHHDM+AP
Sbjct: 61  APSEAEQILQQVLQRRRSSD-SPSDHLVQGFTLDDFFHYLFMDDFNGPIKTQVHHDMSAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIK LK GLRVVELDLWPNSAKDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKGLKSGLRVVELDLWPNSAKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPVTLLKCLKSI +HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ  S
Sbjct: 181 TSPVTLLKCLKSIKEHAFEKSPYPVIITLEDHLTPNLQAKVAEMVTGIFGDTLFYPQAAS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLKYRIIISTKPPKEYLE    KDKVL DGKESSEEETSG ETPDL    E 
Sbjct: 241 LSEFPSPESLKYRIIISTKPPKEYLE----KDKVLSDGKESSEEETSGMETPDL----EA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           EE SESDPDD+E+   E K  QQGAPEYKRLITIHAGKPKGGCLKDAL V GDKVRRLSL
Sbjct: 301 EEQSESDPDDDESIVGELKTFQQGAPEYKRLITIHAGKPKGGCLKDALNVQGDKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV SNG+DVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSNGSDVVRFTQRNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLWLMHGMFRANGGCGYLKKPDFLL KGPQNEVFDP + LPVKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWLMHGMFRANGGCGYLKKPDFLLLKGPQNEVFDPSRPLPVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADA KKKTK IENNWVP+WNEEFKFPLTVPEL L+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAVKKKTKVIENNWVPVWNEEFKFPLTVPELGLLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKK 579
           +EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKK
Sbjct: 541 MEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKK 569

[Close]

BLAST of MS003395 vs. ExPASy TrEMBL
Match: A0A6J1JKG3 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111487722 PE=4 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.2e-291
Identity = 501/594 (84.34%), Postives = 538/594 (90.57%), Query Frame = 0


Query: 1   MKGGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEV 60
           MKGG NSYKV  FSRKFK++EA+PP DV D FA+F+ GG+YM+A+Q+L FL DQQ E E 
Sbjct: 1   MKGGCNSYKVFGFSRKFKLSEAEPPQDVIDIFASFTDGGEYMSAEQMLHFLGDQQ-EMEC 60

Query: 61  TLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAP 120
           TLS+AEQIIEQ+LQKRR SD NPS  S QG +LDDFFHYLFM  FNGPIK++VHHDMNAP
Sbjct: 61  TLSEAEQIIEQILQKRRGSD-NPSGDSSQGLSLDDFFHYLFMPEFNGPIKTQVHHDMNAP 120

Query: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTL 180
           LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKAL RGLRVVELDLWPNS KDDVHVLHGRTL
Sbjct: 121 LSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALNRGLRVVELDLWPNSTKDDVHVLHGRTL 180

Query: 181 TSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDS 240
           TSPV LLKCLKSIN+HAFE SPYPVIITLEDHLTP LQAKVAEMVTGIFGDTLFYPQ DS
Sbjct: 181 TSPVPLLKCLKSINEHAFEKSPYPVIITLEDHLTPDLQAKVAEMVTGIFGDTLFYPQADS 240

Query: 241 LSEFPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEV 300
           LSEFPSPESLK+RIIISTKPPKEYL S  KKDK+L DGKESSEEE+S     D +T F+ 
Sbjct: 241 LSEFPSPESLKHRIIISTKPPKEYLTS-NKKDKILSDGKESSEEESS-----DPVTDFDA 300

Query: 301 EELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSL 360
           +E SESDPD+EE S  ESKP QQGAPEYKRLITIHAGKPKGGCLKDAL V G+KVRRLSL
Sbjct: 301 DEQSESDPDEEENSGGESKPPQQGAPEYKRLITIHAGKPKGGCLKDALNVQGEKVRRLSL 360

Query: 361 SEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYG 420
           SEQ L+KAV S+GTDVVRFTQ N+LRVYPKGTRVTSSNFRPNIGW HGAQMVALNMQGYG
Sbjct: 361 SEQGLEKAVVSDGTDVVRFTQKNILRVYPKGTRVTSSNFRPNIGWTHGAQMVALNMQGYG 420

Query: 421 RSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSD 480
           RSLW+MHGMFRANGGCGYLKKPDFLL K P++E+FDPKK  PVKETLKVTVYLGDGWSSD
Sbjct: 421 RSLWMMHGMFRANGGCGYLKKPDFLLPKSPEDEIFDPKKPPPVKETLKVTVYLGDGWSSD 480

Query: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQ 540
           FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKT+ IEN+WVPIWNEEFKFPLTVPELAL+Q
Sbjct: 481 FSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTRVIENDWVPIWNEEFKFPLTVPELALLQ 540

Query: 541 LEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           +EVRDFDRSEKDDFGGQTCLPI+EL+PGIRAVPLYDKKGEKL SVRLLMRF F+
Sbjct: 541 IEVRDFDRSEKDDFGGQTCLPISELRPGIRAVPLYDKKGEKLTSVRLLMRFLFL 586

[Close]

BLAST of MS003395 vs. TAIR 10
Match: AT2G40116.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 755.4 bits (1949), Expect = 3.5e-218
Identity = 384/611 (62.85%), Postives = 462/611 (75.61%), Query Frame = 0


Query: 3   GGHNSYKVLCFSRKFKITEAQPPPDVRDAFATFSGGG----------------DYMTADQ 62
           G +N      ++RKFKI E  P  DVRDAF  F+ GG                  M A+Q
Sbjct: 15  GSYNYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQ 74

Query: 63  LLRFLVDQQGEEEVTLSQAEQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFN 122
           L  FL D    E  T+++A+++I++V+++R    ++ ++ +  G  LDDFF++LF D  N
Sbjct: 75  LCSFLDDH--GESTTVAEAQRLIDEVIRRR----HHVTRFTRHGLDLDDFFNFLFYDDLN 134

Query: 123 GPIKSKVHHDMNAPLSHYFIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPN 182
            PI   VH DM APLSHYFIYTGHNSYLTGNQLSSDCS+ P+IKAL+RG+RV+ELDLWPN
Sbjct: 135 PPITPHVHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPN 194

Query: 183 SAKDDVHVLHGRTLTSPVTLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVT 242
           S   D++VLHGRTLT+PV L+KCLKSI  +AF +SPYPVIITLEDHLTP LQAKVAEM T
Sbjct: 195 STGTDINVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMAT 254

Query: 243 GIFGDTLFYPQEDSLSEFPSPESLKYRIIISTKPPKEYLESK----KKKDKVLCDGKESS 302
            IFG  L+YP+ DSL EFPSP SL +RIIISTKPPKEYLES+    K+KD  +    E  
Sbjct: 255 QIFGQMLYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDE 314

Query: 303 EEETSGKETPDLMTVFEVEELSESDPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGG 362
              T   +T + M +F+ +  S+SD D E+  A E +      P YKRLITIHAGKPK G
Sbjct: 315 TPRTEEIQTLESM-LFDQDFESKSDSDQEDEEASEDQ-----KPAYKRLITIHAGKPK-G 374

Query: 363 CLKDALKVHGDKVRRLSLSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPN 422
            +K+ +KV  DKVRRLSLSEQ L +  +SN  DVVRFTQ N+LR+YPKGTR  SSN++P 
Sbjct: 375 TVKEEMKVVVDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPL 434

Query: 423 IGWIHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALP 482
           IGW HGAQM+A NMQGYG+SLWLMHGMFRANGGCGY+KKP+FL++KG  +EVFDP+K LP
Sbjct: 435 IGWTHGAQMIAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLP 494

Query: 483 VKETLKVTVYLGDGWSSDFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPI 542
           VKETLKV VY+GDGW  DFS THFD YSPPDFYTK+ IVGVPAD AKKKTK IE+NW PI
Sbjct: 495 VKETLKVKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPI 554

Query: 543 WNEEFKFPLTVPELALMQLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKL 594
           W+EEF FPLTVPELAL+++EVR++D SEKDDFGGQTCLP+ EL+PGIR+VPLYDKKGEK+
Sbjct: 555 WDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKM 612

[Close]

BLAST of MS003395 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 698.4 bits (1801), Expect = 5.1e-201
Identity = 353/592 (59.63%), Postives = 434/592 (73.31%), Query Frame = 0


Query: 7   SYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           +YKV  CF R+F+ T ++ P +++  F  +S  G  MT D L RFL+D Q +++ T   A
Sbjct: 5   TYKVCFCFRRRFRYTASEAPREIKTIFEKYSENG-VMTVDHLHRFLIDVQKQDKATREDA 64

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIK-SKVHHDMNAPLSHY 126
           + II          ++  S     G  LD FF YLF D+ N P+   KVHHDM+AP+SHY
Sbjct: 65  QSII----------NSASSLLHRNGLHLDAFFKYLFGDN-NPPLALHKVHHDMDAPISHY 124

Query: 127 FIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPV 186
           FI+TGHNSYLTGNQLSSDCS+ PII ALK+G+RV+ELD+WPNS KDD+ VLHG TLT+PV
Sbjct: 125 FIFTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPV 184

Query: 187 TLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQ-EDSLSE 246
            L+KCLK+I  HAF+ S YPV++TLEDHLTP LQ+KVAEMVT IFG+ LF P   +SL E
Sbjct: 185 GLIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKE 244

Query: 247 FPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEEL 306
           FPSP SLK RIIISTKPPKEY E K    +V+  GK+  +EE  G+E P  +   + E  
Sbjct: 245 FPSPNSLKRRIIISTKPPKEYKEGKDV--EVVQKGKDLGDEEVWGREVPSFIQRNKSEAK 304

Query: 307 SESD-PDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSLSE 366
            + D  DD++    E K      P+YK LI IHAGKPKGG + + LKV  DKVRRLSLSE
Sbjct: 305 DDLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGG-ITECLKVDPDKVRRLSLSE 364

Query: 367 QALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYGRS 426
           + L+KA       +VRFTQ N+LR+YPKGTRVTSSN+ P +GW HGAQMVA NMQGYGRS
Sbjct: 365 EQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRS 424

Query: 427 LWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSDFS 486
           LWLM GMFRANGGCGY+KKPD LL+ G  +++FDPK  LPVK TL+VTVY+G+GW  DF 
Sbjct: 425 LWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFR 484

Query: 487 QTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQLE 546
            THFD YSPPDFYT++ I GVP D   KKTKT+E+NW+P W+E F+FPLTVPELAL++LE
Sbjct: 485 HTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLE 544

Query: 547 VRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           V ++D SEKDDFGGQTCLP+ EL  GIRA PL+ +KGEK KSV+LL++ EFV
Sbjct: 545 VHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

[Close]

BLAST of MS003395 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 698.4 bits (1801), Expect = 5.1e-201
Identity = 353/592 (59.63%), Postives = 434/592 (73.31%), Query Frame = 0


Query: 7   SYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           +YKV  CF R+F+ T ++ P +++  F  +S  G  MT D L RFL+D Q +++ T   A
Sbjct: 5   TYKVCFCFRRRFRYTASEAPREIKTIFEKYSENG-VMTVDHLHRFLIDVQKQDKATREDA 64

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIK-SKVHHDMNAPLSHY 126
           + II          ++  S     G  LD FF YLF D+ N P+   KVHHDM+AP+SHY
Sbjct: 65  QSII----------NSASSLLHRNGLHLDAFFKYLFGDN-NPPLALHKVHHDMDAPISHY 124

Query: 127 FIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPV 186
           FI+TGHNSYLTGNQLSSDCS+ PII ALK+G+RV+ELD+WPNS KDD+ VLHG TLT+PV
Sbjct: 125 FIFTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPV 184

Query: 187 TLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQ-EDSLSE 246
            L+KCLK+I  HAF+ S YPV++TLEDHLTP LQ+KVAEMVT IFG+ LF P   +SL E
Sbjct: 185 GLIKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKE 244

Query: 247 FPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEEL 306
           FPSP SLK RIIISTKPPKEY E K    +V+  GK+  +EE  G+E P  +   + E  
Sbjct: 245 FPSPNSLKRRIIISTKPPKEYKEGKDV--EVVQKGKDLGDEEVWGREVPSFIQRNKSEAK 304

Query: 307 SESD-PDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLSLSE 366
            + D  DD++    E K      P+YK LI IHAGKPKGG + + LKV  DKVRRLSLSE
Sbjct: 305 DDLDGNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGG-ITECLKVDPDKVRRLSLSE 364

Query: 367 QALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGYGRS 426
           + L+KA       +VRFTQ N+LR+YPKGTRVTSSN+ P +GW HGAQMVA NMQGYGRS
Sbjct: 365 EQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRS 424

Query: 427 LWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSSDFS 486
           LWLM GMFRANGGCGY+KKPD LL+ G  +++FDPK  LPVK TL+VTVY+G+GW  DF 
Sbjct: 425 LWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFR 484

Query: 487 QTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALMQLE 546
            THFD YSPPDFYT++ I GVP D   KKTKT+E+NW+P W+E F+FPLTVPELAL++LE
Sbjct: 485 HTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLE 544

Query: 547 VRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           V ++D SEKDDFGGQTCLP+ EL  GIRA PL+ +KGEK KSV+LL++ EFV
Sbjct: 545 VHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

[Close]

BLAST of MS003395 vs. TAIR 10
Match: AT5G58700.1 (phosphatidylinositol-speciwc phospholipase C4 )

HSP 1 Score: 673.7 bits (1737), Expect = 1.4e-193
Identity = 343/595 (57.65%), Postives = 438/595 (73.61%), Query Frame = 0


Query: 7   SYK-VLCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           SYK  L F+RKF++TE+ P  DVRD F  ++ G  +M+ +QL + + ++ GE E +L +A
Sbjct: 9   SYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLEEA 68

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKSKVHHDMNAPLSHYF 126
           E+I+++VL+++    ++ ++ + +  TLDDF +YLF    N PI  +VH +M+APLSHYF
Sbjct: 69  ERIVDEVLRRK----HHIAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYF 128

Query: 127 IYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPVT 186
           I+TGHNSYLTGNQLSS+CS+ PI  AL+RG+RVVELDLWP    DDV V HGRTLT  V 
Sbjct: 129 IFTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRGT-DDVCVKHGRTLTKEVK 188

Query: 187 LLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQEDSLSEFP 246
           L KCL+SI  +AF  S YPVIITLEDHLTP LQ KVA+M+T  FGD L+Y       EFP
Sbjct: 189 LGKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFP 248

Query: 247 SPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLM-TVFEVEELS 306
           SPE LK +I+ISTKPPKEYLE+   K+K   +  + S+E+  GKE  DL+ T  ++++++
Sbjct: 249 SPEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDEDVWGKEPEDLISTQSDLDKVT 308

Query: 307 ES----DPDDEETSAVES-KPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVRRLS 366
            S    + DDEE  + ES    Q  APEYKRLI IHAGKPKGG L+ ALKV  +K+RRLS
Sbjct: 309 SSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGG-LRMALKVDPNKIRRLS 368

Query: 367 LSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNMQGY 426
           LSEQ L+KAVAS G DV+RFTQ N LR+YPKGTR  SSN++P IGW+ GAQM+A NMQGY
Sbjct: 369 LSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGY 428

Query: 427 GRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDGWSS 486
           GR+LWLM GMFRANGGCGY+KKPDFL+   P  + F PK     K+TLKV V +GDGW  
Sbjct: 429 GRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLL 488

Query: 487 DFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPELALM 546
           DF +THFD+YSPPDF+ ++ I G P D   +KTK   + W PIWN+EF FPL VPELAL+
Sbjct: 489 DFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALL 548

Query: 547 QLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           ++EV + D +EKDDFGGQTCLP++E++ GIRAVPL+++KG K  S RLLMRFEFV
Sbjct: 549 RVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEFV 597

[Close]

BLAST of MS003395 vs. TAIR 10
Match: AT3G55940.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 659.8 bits (1701), Expect = 2.0e-189
Identity = 338/598 (56.52%), Postives = 428/598 (71.57%), Query Frame = 0


Query: 7   SYKV-LCFSRKFKITEAQPPPDVRDAFATFSGGGDYMTADQLLRFLVDQQGEEEVTLSQA 66
           +YKV  CF R+++ T +  P +++  F  +S  G  MT D LLRFL+D Q +++ T  +A
Sbjct: 5   TYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKG-LMTTDLLLRFLIDVQKQDKATKEEA 64

Query: 67  EQIIEQVLQKRRSSDNNPSQPSDQGFTLDDFFHYLFMDSFNGPIKS-KVHHDMNAPLSHY 126
           + I+          + + S     G  LD FF YLF  + N P+ S +VH DM+APLSHY
Sbjct: 65  QDIV----------NASSSLLHRNGLHLDAFFKYLFAVT-NSPLSSLEVHQDMDAPLSHY 124

Query: 127 FIYTGHNSYLTGNQLSSDCSDAPIIKALKRGLRVVELDLWPNSAKDDVHVLHGRTLTSPV 186
           FIYTGHNSYLTGNQLSSDCS+ PII+ALK+G+RV+ELD+WPNS +D + VLHGRTLTSPV
Sbjct: 125 FIYTGHNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPV 184

Query: 187 TLLKCLKSINKHAFETSPYPVIITLEDHLTPGLQAKVAEMVTGIFGDTLFYPQE-DSLSE 246
            L+KCL++I +HAF+ S YPV++TLEDHLTP LQAKVAEMVT IFG+ LF P   + L E
Sbjct: 185 ELIKCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKE 244

Query: 247 FPSPESLKYRIIISTKPPKEYLESKKKKDKVLCDGKESSEEETSGKETPDLMTVFEVEEL 306
           FPSP  LK RI+ISTKPPKEY       D ++  G++  ++E  G+E P  +      + 
Sbjct: 245 FPSPAFLKKRIMISTKPPKEY--KAATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDK 304

Query: 307 SES-------DPDDEETSAVESKPLQQGAPEYKRLITIHAGKPKGGCLKDALKVHGDKVR 366
           ++S       D DD++    + K  +   PEYK LI I AGKPKGG + + LKV  DKVR
Sbjct: 305 NDSNGDDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGG-ITECLKVDPDKVR 364

Query: 367 RLSLSEQALQKAVASNGTDVVRFTQTNVLRVYPKGTRVTSSNFRPNIGWIHGAQMVALNM 426
           RLSLSE+ L+KA       +VRFTQ N+LRVYPKGTR+TSSN+ P I W HGAQMVA NM
Sbjct: 365 RLSLSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNM 424

Query: 427 QGYGRSLWLMHGMFRANGGCGYLKKPDFLLQKGPQNEVFDPKKALPVKETLKVTVYLGDG 486
           QG GRSLW+M GMFR NGGCGY+KKPD LL+    N VFDP+  LPVK TL+VT+Y+G+G
Sbjct: 425 QGLGRSLWVMQGMFRGNGGCGYIKKPDLLLK---SNAVFDPEATLPVKTTLRVTIYMGEG 484

Query: 487 WSSDFSQTHFDNYSPPDFYTKIRIVGVPADAAKKKTKTIENNWVPIWNEEFKFPLTVPEL 546
           W  DF  THFD YSPPDFYT++ I GVPAD   KKTKT+E+NW+P W+E F+FPLTVPEL
Sbjct: 485 WYYDFPHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPEL 544

Query: 547 ALMQLEVRDFDRSEKDDFGGQTCLPITELKPGIRAVPLYDKKGEKLKSVRLLMRFEFV 595
           AL+++EV ++D SEKDDFGGQ CLP+ EL+ GIRAVPL ++ G K +SV+LL+R EFV
Sbjct: 545 ALLRIEVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRLEFV 584

The following BLAST results are available for this feature:

BLAST of MS003395 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134059.1	0.0e+00	100.00	phosphoinositide phospholipase C 6-like [Momordica charantia]	[more]
XP_038884114.1	6.8e-309	87.90	phosphoinositide phospholipase C 6 [Benincasa hispida]	[more]
XP_008441466.1	1.3e-299	86.36	PREDICTED: phosphoinositide phospholipase C 6 [Cucumis melo]	[more]
XP_004138421.1	7.5e-295	85.35	phosphoinositide phospholipase C 6 [Cucumis sativus] >KGN45789.1 hypothetical pr...	[more]
KAA0055041.1	5.9e-292	86.51	phosphoinositide phospholipase C 6 [Cucumis melo var. makuwa] >TYK24436.1 phosph...	[more]

BLAST of MS003395 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GV43	5.0e-217	62.85	Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 ...	[more]
Q39033	7.2e-200	59.63	Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 ...	[more]
Q944C1	1.9e-192	57.65	Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 ...	[more]
Q9LY51	2.8e-188	56.52	Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 ...	[more]
Q944C2	5.0e-185	55.46	Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 ...	[more]

BLAST of MS003395 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXQ4	0.0e+00	100.00	Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111006425 ...	[more]
A0A1S3B496	6.4e-300	86.36	Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103485573 PE=4 SV...	[more]
A0A0A0KAC7	3.6e-295	85.35	Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_6G011650 PE=4...	[more]
A0A5A7UN86	2.9e-292	86.51	Phosphoinositide phospholipase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1JKG3	3.2e-291	84.34	Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111487722 PE=...	[more]

BLAST of MS003395 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40116.1	3.5e-218	62.85	Phosphoinositide-specific phospholipase C family protein	[more]
AT3G08510.1	5.1e-201	59.63	phospholipase C 2	[more]
AT3G08510.2	5.1e-201	59.63	phospholipase C 2	[more]
AT5G58700.1	1.4e-193	57.65	phosphatidylinositol-speciwc phospholipase C4	[more]
AT3G55940.1	2.0e-189	56.52	Phosphoinositide-specific phospholipase C family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001192	Phosphoinositide phospholipase C family	PRINTS	PR00390	PHPHLIPASEC	coord: 243..260 score: 52.62 coord: 146..166 score: 49.42 coord: 384..405 score: 56.86 coord: 560..570 score: 47.34 coord: 405..423 score: 56.6 coord: 120..138 score: 72.29
IPR001192	Phosphoinositide phospholipase C family	PANTHER	PTHR10336	PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN	coord: 7..594
IPR001711	Phospholipase C, phosphatidylinositol-specific, Y domain	SMART	SM00149	plcy_3	coord: 328..446 e-value: 6.8E-56 score: 201.7
IPR001711	Phospholipase C, phosphatidylinositol-specific, Y domain	PFAM	PF00387	PI-PLC-Y	coord: 358..444 e-value: 1.3E-26 score: 93.2
IPR001711	Phospholipase C, phosphatidylinositol-specific, Y domain	PROSITE	PS50008	PIPLC_Y_DOMAIN	coord: 359..445 score: 35.700802
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 466..574 e-value: 4.2E-15 score: 66.2
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 467..568 e-value: 1.1E-13 score: 51.4
IPR000008	C2 domain	PROSITE	PS50004	C2	coord: 447..576 score: 17.743546
IPR000909	Phosphatidylinositol-specific phospholipase C, X domain	SMART	SM00148	plcx_3	coord: 115..259 e-value: 6.4E-67 score: 238.3
IPR000909	Phosphatidylinositol-specific phospholipase C, X domain	PFAM	PF00388	PI-PLC-X	coord: 117..259 e-value: 3.5E-49 score: 166.1
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily	GENE3D	3.20.20.190	Phosphatidylinositol (PI) phosphodiesterase	coord: 112..458 e-value: 4.1E-100 score: 337.0
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily	SUPERFAMILY	51695	PLC-like phosphodiesterases	coord: 115..447
IPR035892	C2 domain superfamily	GENE3D	2.60.40.150	C2 domain	coord: 461..594 e-value: 1.7E-31 score: 111.2
IPR035892	C2 domain superfamily	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 466..593
IPR015359	Phosphoinositide-specific phospholipase C, EF-hand-like domain	PFAM	PF09279	EF-hand_like	coord: 27..100 e-value: 3.0E-6 score: 27.6
None	No IPR available	GENE3D	1.10.238.10		coord: 22..108 e-value: 8.2E-13 score: 50.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..322
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..290
None	No IPR available	PANTHER	PTHR10336:SF101	PHOSPHOINOSITIDE PHOSPHOLIPASE C 6	coord: 7..594
None	No IPR available	PROSITE	PS50007	PIPLC_X_DOMAIN	coord: 115..259 score: 40.535099
None	No IPR available	CDD	cd08599	PI-PLCc_plant	coord: 114..433 e-value: 2.30975E-119 score: 351.289
None	No IPR available	CDD	cd00275	C2_PLC_like	coord: 464..593 e-value: 4.62374E-41 score: 143.066
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003395.1	MS003395.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035556	intracellular signal transduction
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0007165	signal transduction
molecular_function	GO:0004435	phosphatidylinositol phospholipase C activity
molecular_function	GO:0008081	phosphoric diester hydrolase activity