MS003393 (gene) Bitter gourd (TR) v1

Overview
NameMS003393
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold234: 2583249 .. 2586210 (+)
RNA-Seq ExpressionMS003393
SyntenyMS003393
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTATCAGACGTGGAAGCAGTGCTCGAATTCCTCAGAAAAAATGGATTTTCAGAGGCAGAGTCCGCTCTCAGAAGGGATGTGGTCGAAAAATCCGAATTGGGTTCTTTTGATTTTGAGAAATTCTTCTTCCCCATGGTCCCTCCTCCACCTCCGGTCAGAATTCCGTCCACTTCCCGGAAATTGGCCTCTCTTGCCACCGGTGACGGCGACGGCGACCATCATTCTAGATCGAACTCTGTTTCCTCCGACGACGAATTTGTCAGCTTGAGGTCTTCTACCTCCGACGTTTGCTCTTCTGGTAATGATCTTAATCAAAATTGGTCAGAGTTGAAGGTCCTGAGTAAAATTTTAAGATTCTTCACTTGTTAAAAAATTCAAAATGGTATCTTGAAGTTGTCTCAATCATATTGAGTAAAATTAGTGTATTCTCTTGATTTCTTCTGTTGTTAGATGATGAGAGAGAGAGAGAGAGAGAACACTTTTTTTTTTTTAATGTTCTTTCATGTTTAGGCAAATCCATGATCCTATATTTGGCAAACTCAAACTGGGTATAGCAATTGGCATCTAGATTGTTCATAATCTCTTCCAATGTTGCAGAATTCATCAATCCGTATGGTATTCCATCAGATTCTTCTTCAGATATTGCCTCCCAGTTCGACACGGCCCGGGATTACCACGAATTCGATTTGCGAAATGATCTGCTTTGGTACGATGAGAAAGAGGATGTGAATTTCATGACACCCTCCTTTGAGGGTCAGGATTTCTTTGGCTGTCCAAGTGAGGATAAGTTTATAATGACAACAGAGAGAGATGAGCAATTTAGTAGCTCGTCAGCTCCAGAGAAGTCGATTCTCTGTCGTGGAGCTCCCTCGAGCGATGAAAGTTTGATCGAGGTCACAGATCATCCTTATCGTGTCGATAGATATAAGCAACTAGAAGGAGGCTTTGAGGGGTTGTCTCAGGGTTGTGGTGCATATAGTTGTTCAATCCCTTTCTGCAAATGCTGTGATACCAATGGAGAGTTCTATAGCCAGAAGTCTGCAGATTGTAGTTATCTGAATCCAAGTTTTAACGAATCCGATTCCAATGATTTCCACTTGAAAGTCATTAAAGACATCACCGCTAACTGTGATTTAGCTCCTCGTCGAGATTATTCGACTGAAATACATTCTACATCTAACTTCCTTGAAGGGTTTGAAGATCAGAACGACTCCGAAAATAAGGTCCTCGAGAAAAGTTCTACACTTAAGGAAACCAATAGCACTAAAGTCGGAAGTCATGGGGAAGTAGAGGATGAAGAACATGAATCCAAACCAGTTGCAGACAGAGATGGCGTTGCTGCCGACGAGCTTCTGATGTGTAATATCAACGAGGATGAATTCGAAGTATTTGATCTGAGAATTATACATAGAAAGAACAGGTTGGCTATTTTTTTCGCTAAAGTCCAACTAAACAATTAGGCATTTGATTGCTTTTACATTTTAAGTATCTATAGAACTTCATTACAAGTGAACAGAAATTAATTGTTACAAAAAGTCTTCACTTTCTACTCTCTGCTTTGATAGATATTTTAAAGCTTGTGTATTGCAGGTTGAATGAGTTGTTCTCATGTATTATGCAATCTACAGGACTGGGTTTGAAGAGAGCAAGGATTTGCCTATTGTGCTAAATACAGTCCTTGCAGGCAGATACTATGTAACCGAGTACCTCGGATCGGCCGCTTTTAGTAAGGTCGTTCAAGCACACGATCTGCAGACGGGAATAGACGTTTGCCTGAAGATCATTAAAAATGACAAAGATTTTTTTGACCAGAGCTTAGATGAAATCAAACTTCTAAAGCTTGTTAACAAACACGACCCAGCAGATCAGTGCCACATTTTGCGGCTTTACGACTACTTTTATCATCAGGTCTGTTTTATCAGTTGCCCTCGAGCTCAATTTGAAAATTCGACCTTAGTATTGGTAAGGTCCGCTCACTCTTTTTTGTAATGAAAATCTGACAGGAACATCTCTTTATTGTTTGCGAACTACTTCGAGCAAACTTATACGAATTTCAAAAATTCAATCAAGAATCCGGTGGAGACGCTTATTTTACATTTCGCAGGTTGCAGGTATATATGGTCATTTAGGCTAAAACGCTACGCTTTCTCGAGTTTAAGACTAATAAAAATGTTTCCGATTTTCTCTCGACAGATCATTACACGCCAATGTTTGGAGGCATTGGAGTACTTGCATCATTTGGGGATCATTCACTGTGATCTAAAGCCAGAAAACATTCTTATTAAGAGTTACAAAAGATGTGAGATTAAGGTTATTGACCTCGGAAGCAGCTGCTTTCAGTCGGATAACTTGTGCCTATACGTGCAGTCTCGATCCTATAGAGCTCCTGAAGTGATTCTCGGCCTCCCGTACGATCCAAAGATTGATTTGTGGTCTCTTGGTTGTATCTTGGCTGAGCTATGCTCTGGAGAAGTAAGCTTTTCTTACTAGTCTGCTTCTAGCAAAACCGATCGAGTTTATATGTTTGATTTGTTTTCAGGTATTATTTCCAAATGACGCAGTTGTTATGATCCTTGCCCGTATGATCGGAATGTTTGGTCCCATTGACATGGAGATGCTGCTAAAAGGACAGGATACACACAAATACTTCACCAAAGAATATGATCTTTATTATATGAATGAGGTTTGGTTTCAGATTCCCTTAGGAATGAATTTAAAGCATAATTAATGTACTTTTTGTGCCATCAAGATATTTGATGATGACTGAGAGTTTCAATTTCAGGAGACTGAACAAATGGAATTCATCATCCCTGAAGAGTCCTCCTTGGAGAATCATCTGCTGGTTTCCGATGCCGGGTTCATTGATTTTCTTACAAACTTACTCGAGATCAATCCTGAAAGACGACTAACTGCAGAGGAAGCCCTGGCACATCCGTGGCTTTCGCAGTCGTACGAA

mRNA sequence

ATGGCCGTATCAGACGTGGAAGCAGTGCTCGAATTCCTCAGAAAAAATGGATTTTCAGAGGCAGAGTCCGCTCTCAGAAGGGATGTGGTCGAAAAATCCGAATTGGGTTCTTTTGATTTTGAGAAATTCTTCTTCCCCATGGTCCCTCCTCCACCTCCGGTCAGAATTCCGTCCACTTCCCGGAAATTGGCCTCTCTTGCCACCGGTGACGGCGACGGCGACCATCATTCTAGATCGAACTCTGTTTCCTCCGACGACGAATTTGTCAGCTTGAGGTCTTCTACCTCCGACGTTTGCTCTTCTGAATTCATCAATCCGTATGGTATTCCATCAGATTCTTCTTCAGATATTGCCTCCCAGTTCGACACGGCCCGGGATTACCACGAATTCGATTTGCGAAATGATCTGCTTTGGTACGATGAGAAAGAGGATGTGAATTTCATGACACCCTCCTTTGAGGGTCAGGATTTCTTTGGCTGTCCAAGTGAGGATAAGTTTATAATGACAACAGAGAGAGATGAGCAATTTAGTAGCTCGTCAGCTCCAGAGAAGTCGATTCTCTGTCGTGGAGCTCCCTCGAGCGATGAAAGTTTGATCGAGGTCACAGATCATCCTTATCGTGTCGATAGATATAAGCAACTAGAAGGAGGCTTTGAGGGGTTGTCTCAGGGTTGTGGTGCATATAGTTGTTCAATCCCTTTCTGCAAATGCTGTGATACCAATGGAGAGTTCTATAGCCAGAAGTCTGCAGATTGTAGTTATCTGAATCCAAGTTTTAACGAATCCGATTCCAATGATTTCCACTTGAAAGTCATTAAAGACATCACCGCTAACTGTGATTTAGCTCCTCGTCGAGATTATTCGACTGAAATACATTCTACATCTAACTTCCTTGAAGGGTTTGAAGATCAGAACGACTCCGAAAATAAGGTCCTCGAGAAAAGTTCTACACTTAAGGAAACCAATAGCACTAAAGTCGGAAGTCATGGGGAAGTAGAGGATGAAGAACATGAATCCAAACCAGTTGCAGACAGAGATGGCGTTGCTGCCGACGAGCTTCTGATGTGTAATATCAACGAGGATGAATTCGAAGTATTTGATCTGAGAATTATACATAGAAAGAACAGGACTGGGTTTGAAGAGAGCAAGGATTTGCCTATTGTGCTAAATACAGTCCTTGCAGGCAGATACTATGTAACCGAGTACCTCGGATCGGCCGCTTTTAGTAAGGTCGTTCAAGCACACGATCTGCAGACGGGAATAGACGTTTGCCTGAAGATCATTAAAAATGACAAAGATTTTTTTGACCAGAGCTTAGATGAAATCAAACTTCTAAAGCTTGTTAACAAACACGACCCAGCAGATCAGTGCCACATTTTGCGGCTTTACGACTACTTTTATCATCAGGAACATCTCTTTATTGTTTGCGAACTACTTCGAGCAAACTTATACGAATTTCAAAAATTCAATCAAGAATCCGGTGGAGACGCTTATTTTACATTTCGCAGGTTGCAGATCATTACACGCCAATGTTTGGAGGCATTGGAGTACTTGCATCATTTGGGGATCATTCACTGTGATCTAAAGCCAGAAAACATTCTTATTAAGAGTTACAAAAGATGTGAGATTAAGGTTATTGACCTCGGAAGCAGCTGCTTTCAGTCGGATAACTTGTGCCTATACGTGCAGTCTCGATCCTATAGAGCTCCTGAAGTGATTCTCGGCCTCCCGTACGATCCAAAGATTGATTTGTGGTCTCTTGGTTGTATCTTGGCTGAGCTATGCTCTGGAGAAGTATTATTTCCAAATGACGCAGTTGTTATGATCCTTGCCCGTATGATCGGAATGTTTGGTCCCATTGACATGGAGATGCTGCTAAAAGGACAGGATACACACAAATACTTCACCAAAGAATATGATCTTTATTATATGAATGAGGAGACTGAACAAATGGAATTCATCATCCCTGAAGAGTCCTCCTTGGAGAATCATCTGCTGGTTTCCGATGCCGGGTTCATTGATTTTCTTACAAACTTACTCGAGATCAATCCTGAAAGACGACTAACTGCAGAGGAAGCCCTGGCACATCCGTGGCTTTCGCAGTCGTACGAA

Coding sequence (CDS)

ATGGCCGTATCAGACGTGGAAGCAGTGCTCGAATTCCTCAGAAAAAATGGATTTTCAGAGGCAGAGTCCGCTCTCAGAAGGGATGTGGTCGAAAAATCCGAATTGGGTTCTTTTGATTTTGAGAAATTCTTCTTCCCCATGGTCCCTCCTCCACCTCCGGTCAGAATTCCGTCCACTTCCCGGAAATTGGCCTCTCTTGCCACCGGTGACGGCGACGGCGACCATCATTCTAGATCGAACTCTGTTTCCTCCGACGACGAATTTGTCAGCTTGAGGTCTTCTACCTCCGACGTTTGCTCTTCTGAATTCATCAATCCGTATGGTATTCCATCAGATTCTTCTTCAGATATTGCCTCCCAGTTCGACACGGCCCGGGATTACCACGAATTCGATTTGCGAAATGATCTGCTTTGGTACGATGAGAAAGAGGATGTGAATTTCATGACACCCTCCTTTGAGGGTCAGGATTTCTTTGGCTGTCCAAGTGAGGATAAGTTTATAATGACAACAGAGAGAGATGAGCAATTTAGTAGCTCGTCAGCTCCAGAGAAGTCGATTCTCTGTCGTGGAGCTCCCTCGAGCGATGAAAGTTTGATCGAGGTCACAGATCATCCTTATCGTGTCGATAGATATAAGCAACTAGAAGGAGGCTTTGAGGGGTTGTCTCAGGGTTGTGGTGCATATAGTTGTTCAATCCCTTTCTGCAAATGCTGTGATACCAATGGAGAGTTCTATAGCCAGAAGTCTGCAGATTGTAGTTATCTGAATCCAAGTTTTAACGAATCCGATTCCAATGATTTCCACTTGAAAGTCATTAAAGACATCACCGCTAACTGTGATTTAGCTCCTCGTCGAGATTATTCGACTGAAATACATTCTACATCTAACTTCCTTGAAGGGTTTGAAGATCAGAACGACTCCGAAAATAAGGTCCTCGAGAAAAGTTCTACACTTAAGGAAACCAATAGCACTAAAGTCGGAAGTCATGGGGAAGTAGAGGATGAAGAACATGAATCCAAACCAGTTGCAGACAGAGATGGCGTTGCTGCCGACGAGCTTCTGATGTGTAATATCAACGAGGATGAATTCGAAGTATTTGATCTGAGAATTATACATAGAAAGAACAGGACTGGGTTTGAAGAGAGCAAGGATTTGCCTATTGTGCTAAATACAGTCCTTGCAGGCAGATACTATGTAACCGAGTACCTCGGATCGGCCGCTTTTAGTAAGGTCGTTCAAGCACACGATCTGCAGACGGGAATAGACGTTTGCCTGAAGATCATTAAAAATGACAAAGATTTTTTTGACCAGAGCTTAGATGAAATCAAACTTCTAAAGCTTGTTAACAAACACGACCCAGCAGATCAGTGCCACATTTTGCGGCTTTACGACTACTTTTATCATCAGGAACATCTCTTTATTGTTTGCGAACTACTTCGAGCAAACTTATACGAATTTCAAAAATTCAATCAAGAATCCGGTGGAGACGCTTATTTTACATTTCGCAGGTTGCAGATCATTACACGCCAATGTTTGGAGGCATTGGAGTACTTGCATCATTTGGGGATCATTCACTGTGATCTAAAGCCAGAAAACATTCTTATTAAGAGTTACAAAAGATGTGAGATTAAGGTTATTGACCTCGGAAGCAGCTGCTTTCAGTCGGATAACTTGTGCCTATACGTGCAGTCTCGATCCTATAGAGCTCCTGAAGTGATTCTCGGCCTCCCGTACGATCCAAAGATTGATTTGTGGTCTCTTGGTTGTATCTTGGCTGAGCTATGCTCTGGAGAAGTATTATTTCCAAATGACGCAGTTGTTATGATCCTTGCCCGTATGATCGGAATGTTTGGTCCCATTGACATGGAGATGCTGCTAAAAGGACAGGATACACACAAATACTTCACCAAAGAATATGATCTTTATTATATGAATGAGGAGACTGAACAAATGGAATTCATCATCCCTGAAGAGTCCTCCTTGGAGAATCATCTGCTGGTTTCCGATGCCGGGTTCATTGATTTTCTTACAAACTTACTCGAGATCAATCCTGAAAGACGACTAACTGCAGAGGAAGCCCTGGCACATCCGTGGCTTTCGCAGTCGTACGAA

Protein sequence

MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTSRKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQFDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSSAPEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDTNGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEGFEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINEDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESSLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE
Homology
BLAST of MS003393 vs. NCBI nr
Match: XP_022134027.1 (serine/threonine-protein kinase PRP4 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 702/704 (99.72%), Postives = 702/704 (99.72%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS
Sbjct: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS
Sbjct: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT
Sbjct: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           NGEFYSQKSADCSYLNPSFNESDSN FHLKVIKDITANCDLAP RDYSTEIHSTSNFLEG
Sbjct: 241 NGEFYSQKSADCSYLNPSFNESDSNVFHLKVIKDITANCDLAPCRDYSTEIHSTSNFLEG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE 360
           FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE
Sbjct: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE 360

Query: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG 420
           DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG
Sbjct: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG 420

Query: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR 480
           IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR
Sbjct: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR 480

Query: 481 ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR 540
           ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR
Sbjct: 481 ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR 540

Query: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL 600
           CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL
Sbjct: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL 600

Query: 601 FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS 660
           FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS
Sbjct: 601 FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS 660

Query: 661 LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE
Sbjct: 661 LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 704

BLAST of MS003393 vs. NCBI nr
Match: XP_008441468.1 (PREDICTED: serine/threonine-protein kinase prp4 isoform X1 [Cucumis melo] >KAA0055038.1 serine/threonine-protein kinase prp4 isoform X1 [Cucumis melo var. makuwa] >TYK24439.1 serine/threonine-protein kinase prp4 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 606/705 (85.96%), Postives = 645/705 (91.49%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MA SDVEAVL+FL+KNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MASSDVEAVLDFLKKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLA+L     DGD  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLAALT----DGDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+NDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSS S
Sbjct: 121 FDTARDYHEFDLQNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILC  APSSDESLIEV DHPY VDR+ QL+G F GLS+G      S+PFCKCC  
Sbjct: 181 APAKSILCHSAPSSDESLIEVADHPYSVDRFNQLKGSFVGLSEG------SVPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           N EFY++KSADC+YLNPSFNE DSNDF LKV+KDI ANCDL P+ D ST+I+STSNF++G
Sbjct: 241 NEEFYNKKSADCNYLNPSFNEMDSNDFELKVVKDIAANCDLVPQSDSSTDIYSTSNFIQG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAAD-ELLMCNIN 360
           FED NDSENK+LEKS  L   +  KV SHGEVE+E +ES+P    DGVAAD EL M   N
Sbjct: 301 FEDHNDSENKILEKSYILNAADGFKVDSHGEVEEETNESEP---GDGVAADNELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
           +DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFS+VV+AHDL T
Sbjct: 361 DDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSRVVRAHDLHT 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDK+FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKEFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGGDAYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGDAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGLPYDPKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLPYDPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPNDAVVMILARMIG FGPID+EMLLKGQ+THKYFTKEYDL+Y+NEET+QMEFIIPEES
Sbjct: 601 LFPNDAVVMILARMIGFFGPIDLEMLLKGQETHKYFTKEYDLFYINEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D GFIDFLT+LLEINPERR TA+EALAHPWLSQSY+
Sbjct: 661 SLEDHLQVFDPGFIDFLTSLLEINPERRPTAKEALAHPWLSQSYD 692

BLAST of MS003393 vs. NCBI nr
Match: XP_038885602.1 (serine/threonine-protein kinase MRK1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 612/705 (86.81%), Postives = 645/705 (91.49%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAVSDVEAVL+FLRKNGFSEAESAL+RDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MAVSDVEAVLDFLRKNGFSEAESALKRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLA  A  DGD D  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLA--ARSDGDDDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+ND LWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTT+RDEQFSS S
Sbjct: 121 FDTARDYHEFDLQNDFLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTQRDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILCR  PSSDESLIEV      VDRYKQLEGGFEGLS+G      SIPFCKCC  
Sbjct: 181 APAKSILCRSTPSSDESLIEVDG----VDRYKQLEGGFEGLSKG------SIPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           +GEFY+ KSADC+YLNPSFNE DSNDF  KV+KDI ANCDLAP+ D ST+I+S+SNF++ 
Sbjct: 241 HGEFYN-KSADCNYLNPSFNEMDSNDFQSKVVKDIVANCDLAPQCDSSTDIYSSSNFVQE 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAA-DELLMCNIN 360
           FED NDS+NK+LE+S TL   NS KVGS GEV++E +E +PVAD DGVAA DEL M   N
Sbjct: 301 FEDHNDSQNKILERSYTLNAANSFKVGSLGEVQEEANEFEPVADGDGVAADDELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
            DEFEVFDLRIIHRKNRTGFEESKDLPIVLN VLAGRYYVTEYLGSAAFSKVVQAHDL  
Sbjct: 361 NDEFEVFDLRIIHRKNRTGFEESKDLPIVLNKVLAGRYYVTEYLGSAAFSKVVQAHDLHM 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGG AYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGAAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPNDAVVMILARMIG+FGPID+EMLLKGQ+THKYFTKEYDL+YMNEET+QMEFIIPEES
Sbjct: 601 LFPNDAVVMILARMIGLFGPIDLEMLLKGQETHKYFTKEYDLFYMNEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D+GFIDFLTNLLEINPERR TA+EALAHPWLSQSYE
Sbjct: 661 SLEDHLRVFDSGFIDFLTNLLEINPERRPTAKEALAHPWLSQSYE 692

BLAST of MS003393 vs. NCBI nr
Match: XP_011656413.1 (serine/threonine-protein kinase prp4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 598/705 (84.82%), Postives = 642/705 (91.06%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MA SDVEAVL+FL+KNGF+EAESAL+RDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MAGSDVEAVLDFLKKNGFAEAESALKRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RK A+LA    D D  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKFAALA----DVDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+NDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTE DEQ+SS S
Sbjct: 121 FDTARDYHEFDLQNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTESDEQYSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILC  AP SDESLIEV DHPY VDRYKQLEG F GLS+G      S+PFCKCC  
Sbjct: 181 APAKSILCHSAPLSDESLIEVADHPYGVDRYKQLEGSFVGLSEG------SVPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           N EFY++KSADC+YLNPSFNE DSNDF L V+KDI ANCDL P+R+ ST+I+STSNF+EG
Sbjct: 241 NEEFYNKKSADCNYLNPSFNEMDSNDFQLNVVKDIAANCDLVPQRNSSTDIYSTSNFIEG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAA-DELLMCNIN 360
           FED NDS+NK+LEKS  L   +S KV +HGEVE+E +ES+     DG AA DEL M   N
Sbjct: 301 FEDHNDSQNKILEKSYLLNAADSFKVDTHGEVEEETNESE---SGDGAAADDELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
           +DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFS+VV+AHDL  
Sbjct: 361 DDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSRVVRAHDLHM 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDK+FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKEFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGGDAYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGDAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGLPY+PKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLPYNPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPN+AVVMILARMIG+FGPID+EMLLKGQ+THKYFTKEYDL+Y+NEET+QMEFIIPEES
Sbjct: 601 LFPNNAVVMILARMIGLFGPIDLEMLLKGQETHKYFTKEYDLFYINEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D GFIDFLT+LLEINPERR TA+EALAHPWLSQSYE
Sbjct: 661 SLEDHLQVFDLGFIDFLTSLLEINPERRPTAKEALAHPWLSQSYE 692

BLAST of MS003393 vs. NCBI nr
Match: XP_038885603.1 (serine/threonine-protein kinase SRPK isoform X2 [Benincasa hispida])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 602/705 (85.39%), Postives = 635/705 (90.07%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAVSDVEAVL+FLRKNGFSEAESAL+RDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MAVSDVEAVLDFLRKNGFSEAESALKRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLA  A  DGD D  S SNSVSSDDEFVSLRSSTSDVCS          SDSSSDIASQ
Sbjct: 61  RKLA--ARSDGDDDDRSGSNSVSSDDEFVSLRSSTSDVCS----------SDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+ND LWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTT+RDEQFSS S
Sbjct: 121 FDTARDYHEFDLQNDFLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTQRDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILCR  PSSDESLIEV      VDRYKQLEGGFEGLS+G      SIPFCKCC  
Sbjct: 181 APAKSILCRSTPSSDESLIEVDG----VDRYKQLEGGFEGLSKG------SIPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           +GEFY+ KSADC+YLNPSFNE DSNDF  KV+KDI ANCDLAP+ D ST+I+S+SNF++ 
Sbjct: 241 HGEFYN-KSADCNYLNPSFNEMDSNDFQSKVVKDIVANCDLAPQCDSSTDIYSSSNFVQE 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAA-DELLMCNIN 360
           FED NDS+NK+LE+S TL   NS KVGS GEV++E +E +PVAD DGVAA DEL M   N
Sbjct: 301 FEDHNDSQNKILERSYTLNAANSFKVGSLGEVQEEANEFEPVADGDGVAADDELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
            DEFEVFDLRIIHRKNRTGFEESKDLPIVLN VLAGRYYVTEYLGSAAFSKVVQAHDL  
Sbjct: 361 NDEFEVFDLRIIHRKNRTGFEESKDLPIVLNKVLAGRYYVTEYLGSAAFSKVVQAHDLHM 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGG AYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGAAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPNDAVVMILARMIG+FGPID+EMLLKGQ+THKYFTKEYDL+YMNEET+QMEFIIPEES
Sbjct: 601 LFPNDAVVMILARMIGLFGPIDLEMLLKGQETHKYFTKEYDLFYMNEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D+GFIDFLTNLLEINPERR TA+EALAHPWLSQSYE
Sbjct: 661 SLEDHLRVFDSGFIDFLTNLLEINPERRPTAKEALAHPWLSQSYE 682

BLAST of MS003393 vs. ExPASy Swiss-Prot
Match: Q09690 (DYRK-family kinase pom1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pom1 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.4e-53
Identity = 122/325 (37.54%), Postives = 192/325 (59.08%), Query Frame = 0

Query: 378 GFEESK-DLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFD 437
           GF++ + D  +VL   +A RY V ++LG  +F +V++  D +TG  V LKII+N K F  
Sbjct: 679 GFDDERGDYKVVLGDHIAYRYEVVDFLGKGSFGQVLRCIDYETGKLVALKIIRNKKRFHM 738

Query: 438 QSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGD 497
           Q+L E K+L+ + + DP D+  +++  D+FY ++HL +  ELL  NLYE  K N   G  
Sbjct: 739 QALVETKILQKIREWDPLDEYCMVQYTDHFYFRDHLCVATELLGKNLYELIKSNGFKG-- 798

Query: 498 AYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSD 557
                  ++ ITRQ ++ L  L+   +IHCDLKPENIL+    + ++KVID GSSCF+ +
Sbjct: 799 --LPIVVIKSITRQLIQCLTLLNEKHVIHCDLKPENILLCHPFKSQVKVIDFGSSCFEGE 858

Query: 558 NLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGM 617
            +  Y+QSR YR+PEVILG+ Y   ID+WSLGCI+AE+ +G  LFP +     LA ++ +
Sbjct: 859 CVYTYIQSRFYRSPEVILGMGYGTPIDVWSLGCIIAEMYTGFPLFPGENEQEQLACIMEI 918

Query: 618 FGPIDMEMLLKGQDTHKYF-TKEYDLYYMNEETEQMEFIIPEESSLENHLLVSDAGFIDF 677
           FGP D  ++ K      +F +      +++ +        P   SL   L   D  F+ F
Sbjct: 919 FGPPDHSLIDKCSRKKVFFDSSGKPRPFVSSKGVSRR---PFSKSLHQVLQCKDVSFLSF 978

Query: 678 LTNLLEINPERRLTAEEALAHPWLS 701
           +++ L+ +P+ R+T ++A  H +L+
Sbjct: 979 ISDCLKWDPDERMTPQQAAQHDFLT 996

BLAST of MS003393 vs. ExPASy Swiss-Prot
Match: P83102 (Putative dual specificity tyrosine-phosphorylation-regulated kinase 3 homolog OS=Drosophila melanogaster OX=7227 GN=Dyrk3 PE=1 SV=3)

HSP 1 Score: 208.0 bits (528), Expect = 3.5e-52
Identity = 125/322 (38.82%), Postives = 184/322 (57.14%), Query Frame = 0

Query: 393 LAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHD 452
           +A RY + + +G  +F +V++A+D +T   V LKI++N+K F  Q+ +EI++L  + +HD
Sbjct: 272 VAYRYEMLKIIGKGSFGQVIKAYDHKTHEHVALKIVRNEKRFHRQAQEEIRILHHLRRHD 331

Query: 453 PADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCL 512
             +  +I+ ++DYF  + H  I  ELL  NLYE  K N   G    F+ + ++      L
Sbjct: 332 KYNTMNIIHMFDYFTFRNHTCITFELLSINLYELIKKNGFKG----FSLQLVRKFAHSLL 391

Query: 513 EALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV 572
           + L+ L+   IIHCD+KPEN+L+K   R  IKVID GSSCF++  +  Y+QSR YRAPEV
Sbjct: 392 QCLDALYKNDIIHCDMKPENVLLKQQGRSGIKVIDFGSSCFENQRIYTYIQSRFYRAPEV 451

Query: 573 ILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTH 632
           ILG  Y   ID+WSLGCILAEL SG  LFP +     LA +I + G  +  +L   + + 
Sbjct: 452 ILGGKYGRAIDMWSLGCILAELLSGHALFPGENESDQLACIIEVLGMPNKNILASSKRSK 511

Query: 633 KYFT-KEYDLY-YMNEETEQMEFII------------PEESSLENHL-LVSDAGFIDFLT 692
            +F+ K Y  Y  +   ++ M  +I            P   SL   L    D  F++F+ 
Sbjct: 512 SFFSPKGYPRYCTVRTMSDGMVVLIGGQSRRGKQRGPPCSKSLSKALDGCKDPLFLNFIR 571

Query: 693 NLLEINPERRLTAEEALAHPWL 700
             LE + ++RLT  EAL HPWL
Sbjct: 572 GCLEWDADKRLTPSEALKHPWL 589

BLAST of MS003393 vs. ExPASy Swiss-Prot
Match: Q9P6P3 (Serine/threonine-protein kinase ppk15 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ppk15 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 4.6e-52
Identity = 130/343 (37.90%), Postives = 186/343 (54.23%), Query Frame = 0

Query: 388 VLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKL 447
           +L T    +Y + + LG   F +V +  DL+T   V +K+IKN   F++Q + E+ +L+L
Sbjct: 121 LLGTDEGRKYLILDTLGHGTFGQVARCQDLKTQQIVAIKVIKNKPAFYNQCVMEVSILEL 180

Query: 448 V-NKHDPADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGDAYFTFRRLQI 507
           + NK+DP D+ H++RLYD F H+ HL +V ELL  NLYE  K NQ  G         ++ 
Sbjct: 181 LNNKYDPEDKRHLIRLYDQFMHKNHLCLVFELLSINLYELIKQNQFRG----LHLSLVRS 240

Query: 508 ITRQCLEALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSDNLCLYVQSRS 567
              Q L     L    IIHCDLKPENIL++      +KVID GS+C +   +  Y+QSR 
Sbjct: 241 FATQLLSCTSLLKQARIIHCDLKPENILLQDLSSPIVKVIDFGSACHERQTVYTYIQSRF 300

Query: 568 YRAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLL 627
           YR+PEVILGL Y+  ID+WSLGCILAEL  G  LFP ++    L R++ M G     ML 
Sbjct: 301 YRSPEVILGLHYNCGIDMWSLGCILAELFLGLPLFPGNSEYNQLCRIVDMLGNPPTWMLE 360

Query: 628 KGQDTHKYFT-------KEYDLYYM------NEETEQMEFIIPEESSLENHLL------- 687
            G+++ KY+        K Y+L  +      N +TEQ       E +L+  +L       
Sbjct: 361 MGKNSKKYYNSGFVNGRKTYELKSIEQFSIENNKTEQPGKQYFGEKTLDAIVLNYPRRKT 420

Query: 688 ---------VSDAGFIDFLTNLLEINPERRLTAEEALAHPWLS 701
                         FIDF+   LE+NP +R T ++A  HP+++
Sbjct: 421 TPKLTPEEHEERLCFIDFIKQFLELNPLKRWTPDQAKNHPFIT 459

BLAST of MS003393 vs. ExPASy Swiss-Prot
Match: Q54BC9 (Probable serine/threonine-protein kinase dyrk2 OS=Dictyostelium discoideum OX=44689 GN=dyrk2 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.7e-51
Identity = 115/307 (37.46%), Postives = 174/307 (56.68%), Query Frame = 0

Query: 393 LAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHD 452
           +A R+ +   LG  +F +VV+ +D +TG  V LKI++N K F +Q+L EIK+L+ +  +D
Sbjct: 601 IAYRFEIVSILGQGSFCQVVKGYDYKTGEMVALKILRNQKRFHNQALTEIKILEYLKTND 660

Query: 453 PADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCL 512
           P     I+ L +YFY + HL +  ELL  NLY+F K N   G    +    ++    Q L
Sbjct: 661 PNSTASIVHLNNYFYFRNHLILTFELLSMNLYDFLKVNHFQG----YNLNLVRRFGAQIL 720

Query: 513 EALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV 572
            +L +L    IIH DLKPENIL+KS  +  IK+ID GSSCF+++ +  Y+QSR YR+PEV
Sbjct: 721 TSLRFLSKRNIIHADLKPENILLKSPTKSGIKLIDFGSSCFENEQIFTYIQSRFYRSPEV 780

Query: 573 ILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTH 632
           ILG  YD  ID+WSLGCIL E+ +G  LFP       LA ++ + G     ++       
Sbjct: 781 ILGTKYDKSIDIWSLGCILVEIFTGVPLFPGSDEPEQLACIMEVLGAPPKSVIDNSTRKD 840

Query: 633 KYFTKEYDLYYMNEETEQMEFIIPEESSLENHLLVSDAGFIDFLTNLLEINPERRLTAEE 692
            +F  +     +   T    + I  + S ++ +   D  F +F+ + L+  P +R+TAE+
Sbjct: 841 IFFEDDGTPKPVKNSTTGELYTIGTK-SFKDSIRSGDEDFDNFILDCLKWEPSQRITAEQ 900

Query: 693 ALAHPWL 700
            L H W+
Sbjct: 901 GLKHDWI 902

BLAST of MS003393 vs. ExPASy Swiss-Prot
Match: Q9NR20 (Dual specificity tyrosine-phosphorylation-regulated kinase 4 OS=Homo sapiens OX=9606 GN=DYRK4 PE=1 SV=2)

HSP 1 Score: 203.4 bits (516), Expect = 8.6e-51
Identity = 122/330 (36.97%), Postives = 179/330 (54.24%), Query Frame = 0

Query: 375 NRTGFEESKDLPI-VLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKD 434
           ++T F++     + VL+  +A RY V E +G  +F +V +  D +    V LKII+N K 
Sbjct: 81  SKTSFDDEHGFYLKVLHDHIAYRYEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKR 140

Query: 435 FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQES 494
           F  Q+L E+K+L+ + K D  +  +++ + D+FY + H  I  ELL  NLYE  K N   
Sbjct: 141 FHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQ 200

Query: 495 GGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCF 554
           G    F+   ++  T   L+ L+ L    IIHCDLKPENI++    +  +KVID GSSC+
Sbjct: 201 G----FSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCY 260

Query: 555 QSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARM 614
           +   +  Y+QSR YR+PEVILG PYD  ID+WSLGCI AEL +G  LFP +  V  LA +
Sbjct: 261 EHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACI 320

Query: 615 IGMFGPIDMEMLLKGQDTHKYF-TKEYDLYYMNEETEQMEFIIPEESSLENHLLVSDAGF 674
           + + G      +        +F +K +     N   ++     P+   L   L   D  F
Sbjct: 321 MEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKR---YPDSKDLTMVLKTYDTSF 380

Query: 675 IDFLTNLLEINPERRLTAEEALAHPWLSQS 703
           +DFL   L   P  R+T ++AL H W+ QS
Sbjct: 381 LDFLRRCLVWEPSLRMTPDQALKHAWIHQS 403

BLAST of MS003393 vs. ExPASy TrEMBL
Match: A0A6J1BYE4 (serine/threonine-protein kinase PRP4 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006402 PE=4 SV=1)

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 702/704 (99.72%), Postives = 702/704 (99.72%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS
Sbjct: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS
Sbjct: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT
Sbjct: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           NGEFYSQKSADCSYLNPSFNESDSN FHLKVIKDITANCDLAP RDYSTEIHSTSNFLEG
Sbjct: 241 NGEFYSQKSADCSYLNPSFNESDSNVFHLKVIKDITANCDLAPCRDYSTEIHSTSNFLEG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE 360
           FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE
Sbjct: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE 360

Query: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG 420
           DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG
Sbjct: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG 420

Query: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR 480
           IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR
Sbjct: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR 480

Query: 481 ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR 540
           ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR
Sbjct: 481 ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR 540

Query: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL 600
           CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL
Sbjct: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL 600

Query: 601 FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS 660
           FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS
Sbjct: 601 FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS 660

Query: 661 LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE
Sbjct: 661 LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 704

BLAST of MS003393 vs. ExPASy TrEMBL
Match: A0A5D3DMB3 (Serine/threonine-protein kinase prp4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G002410 PE=4 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 606/705 (85.96%), Postives = 645/705 (91.49%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MA SDVEAVL+FL+KNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MASSDVEAVLDFLKKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLA+L     DGD  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLAALT----DGDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+NDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSS S
Sbjct: 121 FDTARDYHEFDLQNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILC  APSSDESLIEV DHPY VDR+ QL+G F GLS+G      S+PFCKCC  
Sbjct: 181 APAKSILCHSAPSSDESLIEVADHPYSVDRFNQLKGSFVGLSEG------SVPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           N EFY++KSADC+YLNPSFNE DSNDF LKV+KDI ANCDL P+ D ST+I+STSNF++G
Sbjct: 241 NEEFYNKKSADCNYLNPSFNEMDSNDFELKVVKDIAANCDLVPQSDSSTDIYSTSNFIQG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAAD-ELLMCNIN 360
           FED NDSENK+LEKS  L   +  KV SHGEVE+E +ES+P    DGVAAD EL M   N
Sbjct: 301 FEDHNDSENKILEKSYILNAADGFKVDSHGEVEEETNESEP---GDGVAADNELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
           +DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFS+VV+AHDL T
Sbjct: 361 DDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSRVVRAHDLHT 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDK+FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKEFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGGDAYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGDAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGLPYDPKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLPYDPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPNDAVVMILARMIG FGPID+EMLLKGQ+THKYFTKEYDL+Y+NEET+QMEFIIPEES
Sbjct: 601 LFPNDAVVMILARMIGFFGPIDLEMLLKGQETHKYFTKEYDLFYINEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D GFIDFLT+LLEINPERR TA+EALAHPWLSQSY+
Sbjct: 661 SLEDHLQVFDPGFIDFLTSLLEINPERRPTAKEALAHPWLSQSYD 692

BLAST of MS003393 vs. ExPASy TrEMBL
Match: A0A1S3B3J6 (serine/threonine-protein kinase prp4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485576 PE=4 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 606/705 (85.96%), Postives = 645/705 (91.49%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MA SDVEAVL+FL+KNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MASSDVEAVLDFLKKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RKLA+L     DGD  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKLAALT----DGDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+NDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSS S
Sbjct: 121 FDTARDYHEFDLQNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILC  APSSDESLIEV DHPY VDR+ QL+G F GLS+G      S+PFCKCC  
Sbjct: 181 APAKSILCHSAPSSDESLIEVADHPYSVDRFNQLKGSFVGLSEG------SVPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           N EFY++KSADC+YLNPSFNE DSNDF LKV+KDI ANCDL P+ D ST+I+STSNF++G
Sbjct: 241 NEEFYNKKSADCNYLNPSFNEMDSNDFELKVVKDIAANCDLVPQSDSSTDIYSTSNFIQG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAAD-ELLMCNIN 360
           FED NDSENK+LEKS  L   +  KV SHGEVE+E +ES+P    DGVAAD EL M   N
Sbjct: 301 FEDHNDSENKILEKSYILNAADGFKVDSHGEVEEETNESEP---GDGVAADNELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
           +DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFS+VV+AHDL T
Sbjct: 361 DDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSRVVRAHDLHT 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDK+FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKEFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGGDAYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGDAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGLPYDPKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLPYDPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPNDAVVMILARMIG FGPID+EMLLKGQ+THKYFTKEYDL+Y+NEET+QMEFIIPEES
Sbjct: 601 LFPNDAVVMILARMIGFFGPIDLEMLLKGQETHKYFTKEYDLFYINEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D GFIDFLT+LLEINPERR TA+EALAHPWLSQSY+
Sbjct: 661 SLEDHLQVFDPGFIDFLTSLLEINPERRPTAKEALAHPWLSQSYD 692

BLAST of MS003393 vs. ExPASy TrEMBL
Match: A0A0A0KDC6 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G011670 PE=4 SV=1)

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 598/705 (84.82%), Postives = 642/705 (91.06%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MA SDVEAVL+FL+KNGF+EAESAL+RDVVEKSELGSFDFEKFFFPMVP PPPVRIPSTS
Sbjct: 1   MAGSDVEAVLDFLKKNGFAEAESALKRDVVEKSELGSFDFEKFFFPMVPAPPPVRIPSTS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RK A+LA    D D  S SNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKFAALA----DVDDRSGSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL+NDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTE DEQ+SS S
Sbjct: 121 FDTARDYHEFDLQNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTESDEQYSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           AP KSILC  AP SDESLIEV DHPY VDRYKQLEG F GLS+G      S+PFCKCC  
Sbjct: 181 APAKSILCHSAPLSDESLIEVADHPYGVDRYKQLEGSFVGLSEG------SVPFCKCCVA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           N EFY++KSADC+YLNPSFNE DSNDF L V+KDI ANCDL P+R+ ST+I+STSNF+EG
Sbjct: 241 NEEFYNKKSADCNYLNPSFNEMDSNDFQLNVVKDIAANCDLVPQRNSSTDIYSTSNFIEG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAA-DELLMCNIN 360
           FED NDS+NK+LEKS  L   +S KV +HGEVE+E +ES+     DG AA DEL M   N
Sbjct: 301 FEDHNDSQNKILEKSYLLNAADSFKVDTHGEVEEETNESE---SGDGAAADDELPMYYTN 360

Query: 361 EDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQT 420
           +DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFS+VV+AHDL  
Sbjct: 361 DDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSRVVRAHDLHM 420

Query: 421 GIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480
           G+DVCLKIIKNDK+FFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL
Sbjct: 421 GVDVCLKIIKNDKEFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELL 480

Query: 481 RANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYK 540
           RANLYEFQKFNQESGGDAYFTFRRLQ+ITRQCLEALE+LHHLGIIHCDLKPENILIKSYK
Sbjct: 481 RANLYEFQKFNQESGGDAYFTFRRLQVITRQCLEALEFLHHLGIIHCDLKPENILIKSYK 540

Query: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEV 600
           RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGLPY+PKIDLWSLGCILAEL SGEV
Sbjct: 541 RCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLPYNPKIDLWSLGCILAELWSGEV 600

Query: 601 LFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEES 660
           LFPN+AVVMILARMIG+FGPID+EMLLKGQ+THKYFTKEYDL+Y+NEET+QMEFIIPEES
Sbjct: 601 LFPNNAVVMILARMIGLFGPIDLEMLLKGQETHKYFTKEYDLFYINEETDQMEFIIPEES 660

Query: 661 SLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           SLE+HL V D GFIDFLT+LLEINPERR TA+EALAHPWLSQSYE
Sbjct: 661 SLEDHLQVFDLGFIDFLTSLLEINPERRPTAKEALAHPWLSQSYE 692

BLAST of MS003393 vs. ExPASy TrEMBL
Match: A0A6J1H1P4 (dual specificity protein kinase YAK1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459623 PE=4 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 578/704 (82.10%), Postives = 619/704 (87.93%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAVSDVEAVL+FLRKNGFSEAESAL+RD+VEKS LGSFDFEKFFFPMVPPPPP+RIPS S
Sbjct: 1   MAVSDVEAVLQFLRKNGFSEAESALKRDMVEKSGLGSFDFEKFFFPMVPPPPPLRIPSVS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120
           RK  +L+ GD DG   S S+SVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ
Sbjct: 61  RKFPALSNGDADGKDRSGSDSVSSDDEFVSLRSSTSDVCSSEFINPYGIPSDSSSDIASQ 120

Query: 121 FDTARDYHEFDLRNDLLWYDEKEDVNFMTPSFEGQDFFGCPSEDKFIMTTERDEQFSSSS 180
           FDTARDYHEFDL     WYDE+EDVNFMTPS EGQD FGCPSEDKFIMTTERDEQFSS S
Sbjct: 121 FDTARDYHEFDLP----WYDEQEDVNFMTPSLEGQDLFGCPSEDKFIMTTERDEQFSSLS 180

Query: 181 APEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCKCCDT 240
           APEKSILC  APSSDESL+EV DHPY VDRY QLEGGFEG  +       SIPFCKCC  
Sbjct: 181 APEKSILCCAAPSSDESLLEVIDHPYCVDRYNQLEGGFEGFPED------SIPFCKCCAA 240

Query: 241 NGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEG 300
           NGEFYSQK+ADC YLNPSFNE+         +KDI  NCD AP RDYS          +G
Sbjct: 241 NGEFYSQKTADCCYLNPSFNET---------VKDIATNCDSAPCRDYS----------KG 300

Query: 301 FEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMCNINE 360
           FED ND +NKV+EKSS L  T+S K GSH EVEDE +ES+  AD DG+AADEL M +I +
Sbjct: 301 FEDHNDPQNKVIEKSSVLNATSSYKDGSH-EVEDEANESEAAADGDGIAADELPMYHICD 360

Query: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTG 420
           DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEY+GSAAFSKVVQAHDLQTG
Sbjct: 361 DEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYIGSAAFSKVVQAHDLQTG 420

Query: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLR 480
           IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQ HILRLYDYFYHQE LFIVCELLR
Sbjct: 421 IDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQHHILRLYDYFYHQERLFIVCELLR 480

Query: 481 ANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKR 540
           +NLYEFQKFNQESGGDAYFTFRRLQ I RQCLEALE+LHHLGIIHCDLKPENIL+KSYKR
Sbjct: 481 SNLYEFQKFNQESGGDAYFTFRRLQFIARQCLEALEFLHHLGIIHCDLKPENILMKSYKR 540

Query: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVL 600
           CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEV+LGL YDPKIDLWSLGCILAEL SGEVL
Sbjct: 541 CEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVMLGLSYDPKIDLWSLGCILAELWSGEVL 600

Query: 601 FPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESS 660
           FPN+AVVMILA+MIG+FGPI+ EMLL GQ+THKYFT+EYDLYYMNEET+QME IIPEE+S
Sbjct: 601 FPNEAVVMILAQMIGLFGPINTEMLLNGQETHKYFTEEYDLYYMNEETDQMELIIPEETS 660

Query: 661 LENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQSYE 705
           LE+HL V D+GF+DFL+NLLEI+PERR TA+EALAHPWLSQSYE
Sbjct: 661 LEDHLQVFDSGFVDFLSNLLEIDPERRPTAKEALAHPWLSQSYE 674

BLAST of MS003393 vs. TAIR 10
Match: AT2G40120.1 (Protein kinase superfamily protein )

HSP 1 Score: 662.9 bits (1709), Expect = 2.8e-190
Identity = 397/706 (56.23%), Postives = 459/706 (65.01%), Query Frame = 0

Query: 1   MAVSDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSFDFEKFFFPMVPPPPPVRIPSTS 60
           MAV DV++VLEFLR+NG +EAESALR D+ EK++L SFDFEKF FP+   P P++I ++S
Sbjct: 1   MAV-DVKSVLEFLRRNGLTEAESALRDDINEKNKLASFDFEKFLFPI---PTPIKITASS 60

Query: 61  RKLASLATGDGDGDHHSRSNSVSSDDEFVSLRSSTSDVCSSE-FINPYGIPSDSSSDIAS 120
           R     +   GDG   S S S SSDDEFVSL SSTS  CSS  F+NPYG  S SSSD  S
Sbjct: 61  RP----SDSGGDG---SNSKSSSSDDEFVSLDSSTSGFCSSSGFVNPYG-DSSSSSDGQS 120

Query: 121 QFDTARDYHEFDLRNDLLWYDEKEDVN-FMTPSFEGQDFFGCP--SEDKFIMTTERDEQF 180
           QF TAR Y E+   ++   ++E ED + FM+P+F   D F  P  +EDKFI     D QF
Sbjct: 121 QFGTARTYPEW---SEFYLHNETEDEDEFMSPAFRESDCFILPENAEDKFI----TDNQF 180

Query: 181 SSSSAPEKSILCRGAPSSDESLIEVTDHPYRVDRYKQLEGGFEGLSQGCGAYSCSIPFCK 240
                 E S+      SS  SL E                                    
Sbjct: 181 ------ENSLGVYDRSSSQGSLTE------------------------------------ 240

Query: 241 CCDTNGEFYSQKSADCSYLNPSFNESDSNDFHLKVIKDITANCDLAPRRDYSTEIHSTSN 300
                        A   YL+  F            + DI                     
Sbjct: 241 -------------ASLDYLDKPF------------LLDI--------------------- 300

Query: 301 FLEGFEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDGVAADELLMC 360
              G ED+ D  +               K G    V DEE +                + 
Sbjct: 301 ---GLEDKTDELD--------------LKTGDQLNVTDEEVD----------------VV 360

Query: 361 NINEDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHD 420
           +  EDE+EVF+LRIIH KNRTGFEE+KDLPIV+NTV+ GRYY+TEY+GSAAFSKVVQA D
Sbjct: 361 HEVEDEYEVFNLRIIHWKNRTGFEENKDLPIVINTVIGGRYYITEYIGSAAFSKVVQAQD 420

Query: 421 LQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVC 480
           L  G+DVCLKIIKNDKDFFDQSLDEIKLLK VNKHDPAD+ HILRLYDYFYHQEHLFIVC
Sbjct: 421 LHNGVDVCLKIIKNDKDFFDQSLDEIKLLKHVNKHDPADEHHILRLYDYFYHQEHLFIVC 480

Query: 481 ELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIK 540
           ELLRANLYEFQKFNQESGG+ YF   RLQ+ITRQCL+AL +LH LGIIHCDLKPENILIK
Sbjct: 481 ELLRANLYEFQKFNQESGGEPYFNLSRLQVITRQCLDALVFLHGLGIIHCDLKPENILIK 540

Query: 541 SYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCS 600
           SYKRC +K+IDLGSSCF+SDNLCLYVQSRSYRAPEVILGLPYD KIDLWSLGCILAELCS
Sbjct: 541 SYKRCAVKIIDLGSSCFRSDNLCLYVQSRSYRAPEVILGLPYDEKIDLWSLGCILAELCS 566

Query: 601 GEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIP 660
           GEVLFPN+AV MILAR++ + GPI+ EML KGQ+THKYFTKEYDLY++NEE+ ++E+II 
Sbjct: 601 GEVLFPNEAVAMILARIVAVLGPIETEMLEKGQETHKYFTKEYDLYHLNEESNEIEYIIT 566

Query: 661 EESSLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPWLSQS 703
           EESSLE  L VSD  F+DF+  LL+INP RR TA EAL HPWLS S
Sbjct: 661 EESSLEEQLQVSDELFLDFVRTLLDINPLRRPTALEALNHPWLSSS 566

BLAST of MS003393 vs. TAIR 10
Match: AT1G73460.1 (Protein kinase superfamily protein )

HSP 1 Score: 531.6 bits (1368), Expect = 9.8e-151
Identity = 317/708 (44.77%), Postives = 431/708 (60.88%), Query Frame = 0

Query: 68   TGDGDGDHHSRSNSVSSDDE-------FVS----LRSSTSDVCSSEFINPYGI----PSD 127
            T  G  DHH R  +  +DD+       ++S    L+S  ++  SSE      +    P+ 
Sbjct: 468  TTRGSPDHHERDANKDADDQSYAEEASYISGEQYLQSKDAEPISSENDRRLTVSEIYPAS 527

Query: 128  SSSDIASQFD----------TARDYHEFD----LRNDLLWYDEKEDVNFMTPSFEGQDFF 187
              +D+ +Q+D          + RD   +       N+LL   +K+ +N    S       
Sbjct: 528  KKNDLLAQYDGHLMDEELLSSMRDEPVWQGFVAQSNELLMLGDKKGINVHRKSHR----- 587

Query: 188  GCPSEDKFIMTTERDEQFS-----SSSAPEKSILCRG--APSSDESLIEVT-DHPYRVDR 247
                +D ++   + D   S     +S A +     R   A  S E   E + DH     R
Sbjct: 588  ----DDVYVEDDQHDSVRSIGVGINSDAADFGSEVRDSLAGGSSEGDFEYSRDHDPVASR 647

Query: 248  YKQLEGGFE-------GLSQGCGAYSCSIPFCKCCDTNGEFYSQKSADCSYLNPSFNESD 307
            +KQL    +         ++   + +    +    D++G F+ +   D  +   S ++ D
Sbjct: 648  FKQLYSESDKKHIDAPNKNKQQASKNDGPDYIADNDSSGSFHVKIQTDGGFSFGS-SQKD 707

Query: 308  SNDFHLKVIKDITANCDLAPRRDYSTEIHSTSNFLEGF--------EDQNDSENKVLEKS 367
                H +  K + +       RD +TE  S S  ++           D + S + V + +
Sbjct: 708  GQSMHAESSKSLWSGNHETVTRDRNTERLSASTAMDDMVATWRRKSSDSSSSHSSVKDNN 767

Query: 368  ST-LKETNST---------KVGSHGEVEDEEHESKPVADRDGVAAD---------ELLMC 427
            +T +K  NS+         +   H + ED+ ++S  + D +  A D         ++   
Sbjct: 768  ATSIKSLNSSPSSLSNYACEERKHADKEDDRNDSSEIEDDNATALDDEEAVAVQEQVRQI 827

Query: 428  NINEDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHD 487
               E+EFE FDL+I+HRKNRTGFEE K+  +VLN+V+AGRY+VTEYLGSAAFSK +QAHD
Sbjct: 828  KAQEEEFETFDLKIVHRKNRTGFEEEKNFNVVLNSVIAGRYHVTEYLGSAAFSKAIQAHD 887

Query: 488  LQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVC 547
            LQTG+DVC+KIIKN+KDFFDQSLDEIKLLK VNKHDPAD+ H+LRLYDYFY++EHL IVC
Sbjct: 888  LQTGMDVCIKIIKNNKDFFDQSLDEIKLLKYVNKHDPADKYHLLRLYDYFYYREHLLIVC 947

Query: 548  ELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIK 607
            ELL+ANLYEF KFN+ESGG+ YFT  RLQ IT QCLE+L++LH LG+IHCDLKPENIL+K
Sbjct: 948  ELLKANLYEFHKFNRESGGEVYFTMPRLQSITIQCLESLQFLHGLGLIHCDLKPENILVK 1007

Query: 608  SYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCS 667
            SY RCEIKVIDLGSSCF++D+LC YVQSRSYRAPEVILGLPYD KID+WSLGCILAELC+
Sbjct: 1008 SYSRCEIKVIDLGSSCFETDHLCSYVQSRSYRAPEVILGLPYDKKIDVWSLGCILAELCT 1067

Query: 668  GEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIP 705
            G VLF ND+   +LAR++G+ G  D EML KG+D+HKYFTK   LY  N+E+ ++E++IP
Sbjct: 1068 GNVLFQNDSPASLLARVMGIVGSFDNEMLTKGRDSHKYFTKNRMLYERNQESNRLEYLIP 1127


HSP 2 Score: 39.7 bits (91), Expect = 1.2e-02
Identity = 17/35 (48.57%), Postives = 25/35 (71.43%), Query Frame = 0

Query: 4  SDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSF 39
          S V+ +LEFLR N FS+AE ALR ++  +S++  F
Sbjct: 5  SSVDGILEFLRNNRFSQAEEALRNELNNRSDINGF 39

BLAST of MS003393 vs. TAIR 10
Match: AT1G73450.1 (Protein kinase superfamily protein )

HSP 1 Score: 525.8 bits (1353), Expect = 5.4e-149
Identity = 319/698 (45.70%), Postives = 425/698 (60.89%), Query Frame = 0

Query: 68   TGDGDGDHHSR-------SNSVSSDDEFVS----LRSSTSDVCSSEFINPYGI----PSD 127
            T  G  DHH R         S + D+ ++S    L+S  ++  SSE      +    P+ 
Sbjct: 463  TTRGSPDHHDRDANKDEDDQSYAEDESYLSGERYLQSKDAEPISSENDRRLTVSEIYPAC 522

Query: 128  SSSDIASQFD-------------TARDYHEFDLR-NDLLWYDEKEDVNFMTPSFEGQDFF 187
              +D+ +Q+D             T   +  F  + N+L+   +K+ +N    S       
Sbjct: 523  KKNDLLAQYDGQLMDEDLLNSMRTEPVWQGFVAQSNELVMLGDKKGINVHRKSH------ 582

Query: 188  GCPSEDKFIMTTERDEQFS-----SSSAPEKSILCRG--APSSDESLIEVT-DHPYRVDR 247
                +D ++   + D   S     +S A +     R   A  S E   E + DH     R
Sbjct: 583  ---LDDVYVEDDQHDSVRSIGVGINSDAADFGSEVRDSLAGGSSEGDFEYSRDHDPVASR 642

Query: 248  YKQLEGGFEGLSQGCGAYSCSIPFCKCCDTNGEFYSQKSADCSYLNPSFNESDSNDFHLK 307
            +KQL    E   +     + +       D+ G F+ +   D  +   S ++ D    H +
Sbjct: 643  FKQLYS--ESDKKHIDGQNKNKQKASKNDSGGSFHVKIQTDGDFSFGS-SQKDGQLMHAE 702

Query: 308  VIKDITANCDLAPRRDYSTEIHSTSNFLEGF--------EDQNDSENKVLEKSST-LKET 367
              K + +       RD +TE+ S S   +           D + S + V E ++T +K  
Sbjct: 703  SSKSLWSGNRETVTRDRNTELLSASTATDDMVATWRQKSSDSSSSRSSVKENNATSIKSV 762

Query: 368  NSTKVG---------SHGEVEDEEHE-----SKPVADRDGVAADE-LLMCNINEDEFEVF 427
            NS+             H E E++  E     +  + D + VA  E +      E+EFE F
Sbjct: 763  NSSPSSLSNYARGERKHAEKENDSSEREDGHATALDDEEAVAVQEQVRQIKAQEEEFETF 822

Query: 428  DLRIIHRKNRTGFEESKDLPIVLNTVLAGRYYVTEYLGSAAFSKVVQAHDLQTGIDVCLK 487
            DL+I+HRKNRTGFEE K+  +VLN+V+AGRY+VTEYLGSAAFSK +QAHDLQTG+DVC+K
Sbjct: 823  DLKIVHRKNRTGFEEEKNFNVVLNSVIAGRYHVTEYLGSAAFSKAIQAHDLQTGMDVCIK 882

Query: 488  IIKNDKDFFDQSLDEIKLLKLVNKHDPADQCHILRLYDYFYHQEHLFIVCELLRANLYEF 547
            IIKN+KDFFDQSLDEIKLLK VNKHDPAD+ H+LRLYDYFY++EHL IVCELL+ANLYEF
Sbjct: 883  IIKNNKDFFDQSLDEIKLLKYVNKHDPADKYHLLRLYDYFYYREHLLIVCELLKANLYEF 942

Query: 548  QKFNQESGGDAYFTFRRLQIITRQCLEALEYLHHLGIIHCDLKPENILIKSYKRCEIKVI 607
             KFN+ESGG+ YFT  RLQ IT QCLE+L++LH LG+IHCDLKPENIL+KSY RCEIKVI
Sbjct: 943  HKFNRESGGEVYFTMPRLQSITIQCLESLQFLHGLGLIHCDLKPENILVKSYSRCEIKVI 1002

Query: 608  DLGSSCFQSDNLCLYVQSRSYRAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAV 667
            DLGSSCF++D+LC YVQSRSYRAPEVILGLPYD KID+WSLGCILAELC+G VLF ND+ 
Sbjct: 1003 DLGSSCFETDHLCSYVQSRSYRAPEVILGLPYDKKIDVWSLGCILAELCTGNVLFQNDSP 1062

Query: 668  VMILARMIGMFGPIDMEMLLKGQDTHKYFTKEYDLYYMNEETEQMEFIIPEESSLENHLL 705
              +LAR++G+ G  D EML KG+D+HKYFTK   LY  N+E+ ++E++IP+ +SL + L 
Sbjct: 1063 ASLLARVMGIVGSFDNEMLTKGRDSHKYFTKNRMLYERNQESNRLEYLIPKRTSLRHRLP 1122


HSP 2 Score: 39.3 bits (90), Expect = 1.5e-02
Identity = 17/35 (48.57%), Postives = 24/35 (68.57%), Query Frame = 0

Query: 4  SDVEAVLEFLRKNGFSEAESALRRDVVEKSELGSF 39
          S V+ +LEFLR N FS AE ALR ++  +S++  F
Sbjct: 5  SSVDGILEFLRNNRFSNAEEALRNELSNRSDINGF 39

BLAST of MS003393 vs. TAIR 10
Match: AT3G17750.1 (Protein kinase superfamily protein )

HSP 1 Score: 523.9 bits (1348), Expect = 2.0e-148
Identity = 263/426 (61.74%), Postives = 324/426 (76.06%), Query Frame = 0

Query: 288  STEIHSTSNFLEGFEDQNDSENKVLEKSSTLKETNSTKVGSHGEVEDEEHESKPVADRDG 347
            ST+  S S+      D +D +      SS    ++S+  G   + EDEE  S    +  G
Sbjct: 709  STKRCSGSSSERNLRDMDDEKVASSRNSSPSALSHSSDTGREHKEEDEEETSHGPEEDPG 768

Query: 348  ---------VAADELLMCNINEDEFEVFDLRIIHRKNRTGFEESKDLPIVLNTVLAGRYY 407
                     V  +++      E +FE F+L+I+HRKNRTGFEE K+  +VLN+V+AGRY+
Sbjct: 769  TSFEDEDAIVVQEQVRQIQAQEQDFETFNLKIVHRKNRTGFEEDKNFHVVLNSVIAGRYH 828

Query: 408  VTEYLGSAAFSKVVQAHDLQTGIDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADQCH 467
            VTE+LGSAAFSK +QAHDL TGIDVC+KIIKN+KDFFDQSLDEIKLLK VN+HDPAD+ H
Sbjct: 829  VTEHLGSAAFSKAIQAHDLHTGIDVCVKIIKNNKDFFDQSLDEIKLLKYVNQHDPADKYH 888

Query: 468  ILRLYDYFYHQEHLFIVCELLRANLYEFQKFNQESGGDAYFTFRRLQIITRQCLEALEYL 527
            +LRLYDYFY +EHL IVCELL+ANLYEFQKFN+ESGG+ YFT  RLQ IT QCLEAL +L
Sbjct: 889  LLRLYDYFYFREHLLIVCELLKANLYEFQKFNRESGGEVYFTMPRLQSITIQCLEALNFL 948

Query: 528  HHLGIIHCDLKPENILIKSYKRCEIKVIDLGSSCFQSDNLCLYVQSRSYRAPEVILGLPY 587
            H LG+IHCDLKPENILIKSY RCEIKVIDLGSSCF++D+LC YVQSRSYRAPEVILGLPY
Sbjct: 949  HGLGLIHCDLKPENILIKSYSRCEIKVIDLGSSCFETDHLCSYVQSRSYRAPEVILGLPY 1008

Query: 588  DPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLLKGQDTHKYFTKE 647
            D KID+WSLGCILAELC+G VLF ND+   +LAR+IG+ G ID EML KG+DT KYFTK 
Sbjct: 1009 DKKIDIWSLGCILAELCTGNVLFQNDSPATLLARVIGIIGSIDQEMLAKGRDTCKYFTKN 1068

Query: 648  YDLYYMNEETEQMEFIIPEESSLENHLLVSDAGFIDFLTNLLEINPERRLTAEEALAHPW 705
            + LY  N+E+  +E++IP++SSL   L + D GFIDF+  LL+++P++R +A EAL HPW
Sbjct: 1069 HLLYERNQESNNLEYLIPKKSSLRRRLPMGDQGFIDFVAYLLQVDPKKRPSAFEALKHPW 1128

BLAST of MS003393 vs. TAIR 10
Match: AT4G03175.1 (Protein kinase superfamily protein )

HSP 1 Score: 206.5 bits (524), Expect = 7.2e-53
Identity = 100/135 (74.07%), Postives = 115/135 (85.19%), Query Frame = 0

Query: 568 RAPEVILGLPYDPKIDLWSLGCILAELCSGEVLFPNDAVVMILARMIGMFGPIDMEMLLK 627
           RAPEVILGLPYD KIDLWSLGCI+AELCSGEVLFPN+AV MILAR++ + GPI+ EML K
Sbjct: 2   RAPEVILGLPYDEKIDLWSLGCIVAELCSGEVLFPNEAVAMILARIVAVLGPIETEMLKK 61

Query: 628 GQDTHKYFTKEYDLYYMNEETEQMEFIIPEESSLENHLLVSDAGFIDFLTNLLEINPERR 687
           GQ+THKYFTKEYDLY++NEE+ ++E+II EESSLE  L VSD  F+DF+  LLEINP RR
Sbjct: 62  GQETHKYFTKEYDLYHLNEESNEIEYIITEESSLEEQLQVSDELFLDFVRTLLEINPLRR 121

Query: 688 LTAEEALAHPWLSQS 703
            TA EAL HPWLS S
Sbjct: 122 PTALEALNHPWLSSS 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134027.10.0e+0099.72serine/threonine-protein kinase PRP4 homolog isoform X1 [Momordica charantia][more]
XP_008441468.10.0e+0085.96PREDICTED: serine/threonine-protein kinase prp4 isoform X1 [Cucumis melo] >KAA00... [more]
XP_038885602.10.0e+0086.81serine/threonine-protein kinase MRK1 isoform X1 [Benincasa hispida][more]
XP_011656413.10.0e+0084.82serine/threonine-protein kinase prp4 isoform X1 [Cucumis sativus][more]
XP_038885603.10.0e+0085.39serine/threonine-protein kinase SRPK isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q096901.4e-5337.54DYRK-family kinase pom1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
P831023.5e-5238.82Putative dual specificity tyrosine-phosphorylation-regulated kinase 3 homolog OS... [more]
Q9P6P34.6e-5237.90Serine/threonine-protein kinase ppk15 OS=Schizosaccharomyces pombe (strain 972 /... [more]
Q54BC91.7e-5137.46Probable serine/threonine-protein kinase dyrk2 OS=Dictyostelium discoideum OX=44... [more]
Q9NR208.6e-5136.97Dual specificity tyrosine-phosphorylation-regulated kinase 4 OS=Homo sapiens OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1BYE40.0e+0099.72serine/threonine-protein kinase PRP4 homolog isoform X1 OS=Momordica charantia O... [more]
A0A5D3DMB30.0e+0085.96Serine/threonine-protein kinase prp4 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3B3J60.0e+0085.96serine/threonine-protein kinase prp4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KDC60.0e+0084.82Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G011... [more]
A0A6J1H1P40.0e+0082.10dual specificity protein kinase YAK1-like isoform X1 OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
AT2G40120.12.8e-19056.23Protein kinase superfamily protein [more]
AT1G73460.19.8e-15144.77Protein kinase superfamily protein [more]
AT1G73450.15.4e-14945.70Protein kinase superfamily protein [more]
AT3G17750.12.0e-14861.74Protein kinase superfamily protein [more]
AT4G03175.17.2e-5374.07Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 397..699
e-value: 3.3E-69
score: 245.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 399..699
e-value: 5.3E-52
score: 176.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 397..699
score: 36.72509
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 451..699
e-value: 9.0E-94
score: 316.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 392..478
e-value: 9.0E-94
score: 316.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..88
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..344
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..82
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..344
NoneNo IPR availablePANTHERPTHR24058DUAL SPECIFICITY PROTEIN KINASEcoord: 4..703
NoneNo IPR availablePANTHERPTHR24058:SF113HYPOTHETICAL SER-THR PROTEIN KINASE-RELATEDcoord: 4..703
NoneNo IPR availableCDDcd14133PKc_DYRK_likecoord: 397..699
e-value: 6.9148E-139
score: 405.884
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 523..535
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 386..701

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003393.1MS003393.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity