MS003360 (gene) Bitter gourd (TR) v1

Overview
NameMS003360
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionadenine/guanine permease AZG1
Locationscaffold234: 2346971 .. 2348710 (-)
RNA-Seq ExpressionMS003360
SyntenyMS003360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTGAACCCAATACGACGTCGCATCATCCGGACCGGATTTCCCGGCTGAACGACGCTGCGGCCAAAACTTGGATCGGAAAGCGGTTCAAATTGGCGGAGAGAAATTCGAGCTTCACCACCGAGCTCCGGGCCGGCACCGCCACCTTCCTCACCATGGCTTACATCTTGGCCGTCAACGCCTCCATCCTCACCGACTCCGGCGGAACCTGCTCTTACTCCGATTGCGTCCCTCTCTGTTCCGACCCTTCCGTCTCTGTTCCCAATTGTTCCGGGTCCAATATCCGGGTCATCCAACCCGACGCGGGCTGTATGTTCGACCCGGTTAATCCTGGGTACGCCAATTGCCTAGACGGAATCCGGCGAGACTTAATTGTAGCCACCATCGCTTCGTCGTTGATCGGTTGCGTTATAATGGGAGTTTTCGCGAATCTTCCCCTGGCTCTGGCGCCCGGAATGGGGACCAACGCTTATTTCGCTTACACCGTCGTCGGATTCCATGGTTCCGGCAATATCTCCTACCGGAGTGCTCTGACCGCCGTCTTCATCGAGGGATTAATCTTCCTCTTCATTTCCGCAATCGGATTTAGGGCCAAACTCGCCAAACTCGTCCCGAGGCCGGTCCGAATCAGTTCCTCTGCCGGAATCGGGCTATTTCTGGCCTTCATCGGATTACAGAGCAGCCAAGGAATCGGGCTAATCGGCTTCAGCCCGTCGACGTTGGTGACGATCGGAGCCTGCCCGGAATCGGCACGTGTCTCGGTCGCACCCGTGGTGACGTCACCGAACGGCACGGTGAGTCTAATGACAGGAGGAACGGTTTCCGGCGGAATCCTCTGCACGAGCGGGCGGATGGAGAGCCCAACGTTGTGGCTGGGCGTGGTGGGATTCGTGATAATTGGTTACTGCCTGGTGAAGAACATCAAAGGGGCAATGATTTACGGGATCGTGTTCGTGACGGCGGTTTCGTGGTTCCGTAAAACGAGCGTGACGGCGTTTCCGGCGACGGCGGCGGGGGACGCGGCGTATGAGTACTTTAAGAAAGTAGTGGATATACACACGATTAAAAGTACAGCTGGAGCACTCAGCTTTAGGGATTTGGGGAAGGCCCACTTTTGGGAAGCAATGGTCACTTTCTTGTACGTGGATATTTTGGATACCACCGGTACCCTTTATTCCATGGCTCGATTCGCCGGATTCGTTGATCGGAACGGCGATTTCGAGGGGCAGTACTTTGCGTTTATGTCCGACGCCACGGCCATTGTGGTGGGTTCGTTGCTGGGGACGTCGCCGGTGACGGCGTTCATTGAGTCGTCCACGGGGATAAGGGAGGGGGGGAGGACGGGGCTGACGGCGTTGACGGTGGCCGGGTATTTCTTATTGGCGTTTTTTTTTACGCCGCTGTTGGCGTCGATTCCGTCGTGGGCGGTGGGGCCGCCGCTGATATTGGTGGGGGTGTTGATGATGAAGGCGGTGGTGGAGATTGAATGGAGTGATATGAGGCAGGCGATTCCGGCGTTCATGACGTTGATTTTGATGCCGTTGACTTATTCAATAGCGTATGGTTTGATTGGTGGAATAGGGACTTACGTGGTGCTGCATGCATGGGATTGGAGCCTGGCGGGTTTCCGAAAATGTGGACTCCTGAAGAAGAAAACTGAGGTCAGCGGATCCTCATCCAACGGGCAGCCGCAGGCAGAAGAAGATCCTGCCCGAAAGACCCTAGGGAGCTACCCG

mRNA sequence

ATGGAAATTGAACCCAATACGACGTCGCATCATCCGGACCGGATTTCCCGGCTGAACGACGCTGCGGCCAAAACTTGGATCGGAAAGCGGTTCAAATTGGCGGAGAGAAATTCGAGCTTCACCACCGAGCTCCGGGCCGGCACCGCCACCTTCCTCACCATGGCTTACATCTTGGCCGTCAACGCCTCCATCCTCACCGACTCCGGCGGAACCTGCTCTTACTCCGATTGCGTCCCTCTCTGTTCCGACCCTTCCGTCTCTGTTCCCAATTGTTCCGGGTCCAATATCCGGGTCATCCAACCCGACGCGGGCTGTATGTTCGACCCGGTTAATCCTGGGTACGCCAATTGCCTAGACGGAATCCGGCGAGACTTAATTGTAGCCACCATCGCTTCGTCGTTGATCGGTTGCGTTATAATGGGAGTTTTCGCGAATCTTCCCCTGGCTCTGGCGCCCGGAATGGGGACCAACGCTTATTTCGCTTACACCGTCGTCGGATTCCATGGTTCCGGCAATATCTCCTACCGGAGTGCTCTGACCGCCGTCTTCATCGAGGGATTAATCTTCCTCTTCATTTCCGCAATCGGATTTAGGGCCAAACTCGCCAAACTCGTCCCGAGGCCGGTCCGAATCAGTTCCTCTGCCGGAATCGGGCTATTTCTGGCCTTCATCGGATTACAGAGCAGCCAAGGAATCGGGCTAATCGGCTTCAGCCCGTCGACGTTGGTGACGATCGGAGCCTGCCCGGAATCGGCACGTGTCTCGGTCGCACCCGTGGTGACGTCACCGAACGGCACGGTGAGTCTAATGACAGGAGGAACGGTTTCCGGCGGAATCCTCTGCACGAGCGGGCGGATGGAGAGCCCAACGTTGTGGCTGGGCGTGGTGGGATTCGTGATAATTGGTTACTGCCTGGTGAAGAACATCAAAGGGGCAATGATTTACGGGATCGTGTTCGTGACGGCGGTTTCGTGGTTCCGTAAAACGAGCGTGACGGCGTTTCCGGCGACGGCGGCGGGGGACGCGGCGTATGAGTACTTTAAGAAAGTAGTGGATATACACACGATTAAAAGTACAGCTGGAGCACTCAGCTTTAGGGATTTGGGGAAGGCCCACTTTTGGGAAGCAATGGTCACTTTCTTGTACGTGGATATTTTGGATACCACCGGTACCCTTTATTCCATGGCTCGATTCGCCGGATTCGTTGATCGGAACGGCGATTTCGAGGGGCAGTACTTTGCGTTTATGTCCGACGCCACGGCCATTGTGGTGGGTTCGTTGCTGGGGACGTCGCCGGTGACGGCGTTCATTGAGTCGTCCACGGGGATAAGGGAGGGGGGGAGGACGGGGCTGACGGCGTTGACGGTGGCCGGGTATTTCTTATTGGCGTTTTTTTTTACGCCGCTGTTGGCGTCGATTCCGTCGTGGGCGGTGGGGCCGCCGCTGATATTGGTGGGGGTGTTGATGATGAAGGCGGTGGTGGAGATTGAATGGAGTGATATGAGGCAGGCGATTCCGGCGTTCATGACGTTGATTTTGATGCCGTTGACTTATTCAATAGCGTATGGTTTGATTGGTGGAATAGGGACTTACGTGGTGCTGCATGCATGGGATTGGAGCCTGGCGGGTTTCCGAAAATGTGGACTCCTGAAGAAGAAAACTGAGGTCAGCGGATCCTCATCCAACGGGCAGCCGCAGGCAGAAGAAGATCCTGCCCGAAAGACCCTAGGGAGCTACCCG

Coding sequence (CDS)

ATGGAAATTGAACCCAATACGACGTCGCATCATCCGGACCGGATTTCCCGGCTGAACGACGCTGCGGCCAAAACTTGGATCGGAAAGCGGTTCAAATTGGCGGAGAGAAATTCGAGCTTCACCACCGAGCTCCGGGCCGGCACCGCCACCTTCCTCACCATGGCTTACATCTTGGCCGTCAACGCCTCCATCCTCACCGACTCCGGCGGAACCTGCTCTTACTCCGATTGCGTCCCTCTCTGTTCCGACCCTTCCGTCTCTGTTCCCAATTGTTCCGGGTCCAATATCCGGGTCATCCAACCCGACGCGGGCTGTATGTTCGACCCGGTTAATCCTGGGTACGCCAATTGCCTAGACGGAATCCGGCGAGACTTAATTGTAGCCACCATCGCTTCGTCGTTGATCGGTTGCGTTATAATGGGAGTTTTCGCGAATCTTCCCCTGGCTCTGGCGCCCGGAATGGGGACCAACGCTTATTTCGCTTACACCGTCGTCGGATTCCATGGTTCCGGCAATATCTCCTACCGGAGTGCTCTGACCGCCGTCTTCATCGAGGGATTAATCTTCCTCTTCATTTCCGCAATCGGATTTAGGGCCAAACTCGCCAAACTCGTCCCGAGGCCGGTCCGAATCAGTTCCTCTGCCGGAATCGGGCTATTTCTGGCCTTCATCGGATTACAGAGCAGCCAAGGAATCGGGCTAATCGGCTTCAGCCCGTCGACGTTGGTGACGATCGGAGCCTGCCCGGAATCGGCACGTGTCTCGGTCGCACCCGTGGTGACGTCACCGAACGGCACGGTGAGTCTAATGACAGGAGGAACGGTTTCCGGCGGAATCCTCTGCACGAGCGGGCGGATGGAGAGCCCAACGTTGTGGCTGGGCGTGGTGGGATTCGTGATAATTGGTTACTGCCTGGTGAAGAACATCAAAGGGGCAATGATTTACGGGATCGTGTTCGTGACGGCGGTTTCGTGGTTCCGTAAAACGAGCGTGACGGCGTTTCCGGCGACGGCGGCGGGGGACGCGGCGTATGAGTACTTTAAGAAAGTAGTGGATATACACACGATTAAAAGTACAGCTGGAGCACTCAGCTTTAGGGATTTGGGGAAGGCCCACTTTTGGGAAGCAATGGTCACTTTCTTGTACGTGGATATTTTGGATACCACCGGTACCCTTTATTCCATGGCTCGATTCGCCGGATTCGTTGATCGGAACGGCGATTTCGAGGGGCAGTACTTTGCGTTTATGTCCGACGCCACGGCCATTGTGGTGGGTTCGTTGCTGGGGACGTCGCCGGTGACGGCGTTCATTGAGTCGTCCACGGGGATAAGGGAGGGGGGGAGGACGGGGCTGACGGCGTTGACGGTGGCCGGGTATTTCTTATTGGCGTTTTTTTTTACGCCGCTGTTGGCGTCGATTCCGTCGTGGGCGGTGGGGCCGCCGCTGATATTGGTGGGGGTGTTGATGATGAAGGCGGTGGTGGAGATTGAATGGAGTGATATGAGGCAGGCGATTCCGGCGTTCATGACGTTGATTTTGATGCCGTTGACTTATTCAATAGCGTATGGTTTGATTGGTGGAATAGGGACTTACGTGGTGCTGCATGCATGGGATTGGAGCCTGGCGGGTTTCCGAAAATGTGGACTCCTGAAGAAGAAAACTGAGGTCAGCGGATCCTCATCCAACGGGCAGCCGCAGGCAGAAGAAGATCCTGCCCGAAAGACCCTAGGGAGCTACCCG

Protein sequence

MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWSLAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTLGSYP
Homology
BLAST of MS003360 vs. NCBI nr
Match: KAG6602590.1 (Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033268.1 Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 489/576 (84.90%), Postives = 530/576 (92.01%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL+ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLSERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSGSN+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGSNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. NCBI nr
Match: XP_023553604.1 (adenine/guanine permease AZG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 489/576 (84.90%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLTERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSGSN+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGSNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. NCBI nr
Match: XP_023553610.1 (adenine/guanine permease AZG1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.1 bits (2478), Expect = 1.7e-275
Identity = 489/576 (84.90%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLTERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSGSN+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGSNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. NCBI nr
Match: XP_022964638.1 (adenine/guanine permease AZG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 957.6 bits (2474), Expect = 4.9e-275
Identity = 488/576 (84.72%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL+ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLSERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSG N+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGPNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. NCBI nr
Match: XP_022964639.1 (adenine/guanine permease AZG1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 957.6 bits (2474), Expect = 4.9e-275
Identity = 488/576 (84.72%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL+ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLSERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSG N+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGPNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. ExPASy Swiss-Prot
Match: Q9SRK7 (Adenine/guanine permease AZG1 OS=Arabidopsis thaliana OX=3702 GN=AZG1 PE=2 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 6.7e-251
Identity = 435/568 (76.58%), Postives = 498/568 (87.68%), Query Frame = 0

Query: 2   EIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVN 61
           ++   TT   P  ++RLN     + +GKRFKLAERNS+FTTELRAGTATFLTMAYILAVN
Sbjct: 8   QLPSTTTRPKPKLLNRLNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVN 67

Query: 62  ASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGI 121
           ASIL+DSGGTCS SDC+PLCS+P++    C+G  +R+IQPD  C F+PVNPGYA C++ I
Sbjct: 68  ASILSDSGGTCSVSDCIPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEI 127

Query: 122 RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTA 181
           R+DLIVAT+A+SLIGCVIMG+ ANLPLALAPGMGTNAYFAYTVVGFHGSG+ISYR+AL A
Sbjct: 128 RKDLIVATVAASLIGCVIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAA 187

Query: 182 VFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPST 241
           VFIEGLIFLFISAIGFRAKLAKLVP+PVRISSSAGIGLFLAFIGLQ++QGIGL+G+SPST
Sbjct: 188 VFIEGLIFLFISAIGFRAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPST 247

Query: 242 LVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVII 301
           LVT+ ACP S+R+S+APV+TS NGTVSL+ GG+VSG I+C  GRMESPT WLG+VGFVII
Sbjct: 248 LVTLAACPASSRISLAPVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVII 307

Query: 302 GYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAG 361
            YCLVKN+KGAMIYGIVFVTAVSWFR T VTAFP T+AGDAA++YFKK+VD+H IK TAG
Sbjct: 308 AYCLVKNVKGAMIYGIVFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAG 367

Query: 362 ALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATA 421
           ALSF  + K HFWEA+VTFLYVDILDTTGTLYSMARFAGFVD  GDF GQYFAFMSDA+A
Sbjct: 368 ALSFSGINKGHFWEALVTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASA 427

Query: 422 IVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPP 481
           IV+GSLLGTSPVT FIESSTGIREGGRTGLTA+TVA YFLLA FFTPLLASIP+WAVGPP
Sbjct: 428 IVIGSLLGTSPVTVFIESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPP 487

Query: 482 LILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWSL 541
           LILVGV+MMK+V EI+W DMR+AIPAF+T+ILMPLTYS+AYGLIGGIG+YVVLH WDW  
Sbjct: 488 LILVGVMMMKSVTEIDWEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGE 547

Query: 542 AGFRKCGLLKKKTEVSGSSSNGQPQAEE 570
            G  K G LK+K +    ++NG  +A E
Sbjct: 548 EGLVKLGFLKRKVK-EEDNNNGVVKASE 574

BLAST of MS003360 vs. ExPASy Swiss-Prot
Match: Q84MA8 (Adenine/guanine permease AZG2 OS=Arabidopsis thaliana OX=3702 GN=AZG2 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 5.0e-145
Identity = 268/526 (50.95%), Postives = 355/526 (67.49%), Query Frame = 0

Query: 13  DRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTDSGGTC 72
           D    LND  +K++IG+ FKL  R ++FTTELRA TATFLTMAYI+ VNA+IL DSG TC
Sbjct: 13  DMKKHLNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSGATC 72

Query: 73  SYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIVATIAS 132
           S +DC  + S                  P   C+    NPGY  C+  +++DL+VAT  S
Sbjct: 73  SINDCSTVASSS---------------PPGPECVLGS-NPGYEQCISRVKKDLVVATSLS 132

Query: 133 SLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGLIFLFI 192
           +++G + MG+ ANLP  LAPGMG NAY AY VVGF GSG+ISY +A+  V +EG  FL +
Sbjct: 133 AMVGSLAMGLLANLPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAV 192

Query: 193 SAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGACPESA 252
           SA+G R KLA+L+P+ VR++ + GIG+F+AF+GLQ +QGIGL+G   STLVT+ AC E+ 
Sbjct: 193 SALGLRGKLARLIPQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTACAETD 252

Query: 253 RVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVKNIKGA 312
            V+ A                       C  G+M+SPT WL VVGF+I  + L+KN+KG+
Sbjct: 253 PVTGA-----------------------CLGGKMKSPTFWLAVVGFLITSFGLMKNVKGS 312

Query: 313 MIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRDLGKAH 372
           MIYGIVFVTA+SW R T VT FP T  GD+ Y YF K+VD H I+ST GA+SF +  K+ 
Sbjct: 313 MIYGIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFTKIVDFHKIQSTLGAISFTEFRKSE 372

Query: 373 FWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSLLGTSP 432
            W A  T  YVD+L TTG LY+MA   GFV+ +G FEG+Y A++ DA + VVGS LG + 
Sbjct: 373 VWVAFATLFYVDLLGTTGVLYTMAEIGGFVE-DGKFEGEYAAYLVDAGSSVVGSALGVTT 432

Query: 433 VTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGVLMMKA 492
              F+ESS G++EGG+TGLTA+ V  YFL + FFTPL+ ++P WAVGP L++VGV+MM  
Sbjct: 433 TATFVESSAGLKEGGKTGLTAVIVGLYFLASMFFTPLVTNVPRWAVGPSLVMVGVMMMGV 492

Query: 493 VVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWD 539
           V +I W + ++A+ AF+T++LMPLTYSIA G+I GIG Y+ L  +D
Sbjct: 493 VKDIRWGETKEAVTAFVTILLMPLTYSIANGIIAGIGIYLALSMYD 498

BLAST of MS003360 vs. ExPASy Swiss-Prot
Match: L7WRR4 (Efflux pump notK' OS=Aspergillus versicolor OX=46472 GN=notK' PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 3.1e-107
Identity = 230/543 (42.36%), Postives = 320/543 (58.93%), Query Frame = 0

Query: 11  HPDRISRLNDAAAKTWIGKRFKL-------AERNSSFTTELRAGTATFLTMAYILAVNAS 70
           H + I R N A A++ +GK F+L         + + F TELRAG ATF  MAYI++VNA+
Sbjct: 3   HAEWIGRTNTAVARSAVGKWFRLEGSGHPRERKGAYFFTELRAGLATFFAMAYIISVNAN 62

Query: 71  ILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRR 130
           I +D+G TC       +C    +   +C+                  N  Y  C   + R
Sbjct: 63  ITSDTGATC-------VCPAEDLET-HCNN-----------------NTEYLLCKQEVNR 122

Query: 131 DLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVF 190
           D++ AT A + +    +G+ ANLP+ALAPGMG NAYFAYTVVG HGSG I Y  A+TAVF
Sbjct: 123 DIVTATAAIASVASFFLGLLANLPVALAPGMGLNAYFAYTVVGHHGSGLIPYSLAVTAVF 182

Query: 191 IEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLV 250
           +EG IFL ++ +G R  LA+ +P  +++++ AGIGL+L  IGL  S G+GL+  +  + +
Sbjct: 183 VEGWIFLGLTMLGIRQWLARAIPASIKLATGAGIGLYLTLIGLSYSAGLGLVQGAQDSPI 242

Query: 251 TIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVV--GFVII 310
            +  C                           S G+  +  +M +PT+W+G+   GF  +
Sbjct: 243 QLAGCASDE---------------------FDSDGLCPSYAKMRNPTMWIGIFCGGFFTV 302

Query: 311 GYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAG 370
            + ++  +KGA+I GI+ V+ +SW R T VT FP T  GD+ +++FKKVV  H I+ T  
Sbjct: 303 -FLMMYRVKGAVIAGILLVSIISWPRTTPVTYFPHTTEGDSMFDFFKKVVTFHPIQHTLV 362

Query: 371 ALSFR-DLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVD-RNGDFEGQYFAFMSDA 430
           A  +        F  A++TFLYVDILD TGTLYSMA+FAG +D R  DFEG   A+  DA
Sbjct: 363 AQDWNISSNGGQFGLALITFLYVDILDATGTLYSMAKFAGAMDERTQDFEGSAMAYTVDA 422

Query: 431 TAIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVG 490
             I +GSL G+ PVTAF+ES  GI EGG+TGLT+      F +A FF P+ ASIP WA G
Sbjct: 423 ICISIGSLFGSPPVTAFVESGAGISEGGKTGLTSCMTGICFFIAVFFAPIFASIPPWATG 482

Query: 491 PPLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDW 543
             L++VG +MM A +EI W  M  AIPAF+T+ +MP TYSIA GLI GI +Y++++   W
Sbjct: 483 STLVIVGSMMMHATLEINWRYMGDAIPAFLTISVMPFTYSIADGLIAGIISYILINGGVW 498

BLAST of MS003360 vs. ExPASy Swiss-Prot
Match: O94300 (Putative xanthine/uracil permease C887.17 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC887.17 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.9e-104
Identity = 224/523 (42.83%), Postives = 307/523 (58.70%), Query Frame = 0

Query: 23  AKTWIGKRFKL-------AERNSSFTTELRAGTATFLTMAYILAVNASILTDSGGTCSYS 82
           A++  G+ F+L         + S F+ E+ AG  TF  MAYILAVNA+IL D+GGTC   
Sbjct: 19  ARSAFGRWFRLEGCGHPRERKGSRFSLEISAGLTTFFAMAYILAVNATILVDTGGTC--- 78

Query: 83  DCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIVATIASSLI 142
                          C+ +N    + D   + D     Y  C +   RDL+ AT A S +
Sbjct: 79  --------------ECTEAN----RDDCDKLDD-----YVLCKEDFHRDLVTATAAISAL 138

Query: 143 GCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGLIFLFISAI 202
               MG+FAN+P+ +APGMG NAYFAY VVG++G+G +SYR AL AVF+EG IF  ++ I
Sbjct: 139 ASFCMGLFANMPVGMAPGMGLNAYFAYQVVGYNGTGRVSYREALLAVFVEGFIFTGLTVI 198

Query: 203 GFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGACPESARVS 262
           G R  LA+++P  ++ ++ AGIGL+L  IGL  S G+G+IG S S +V +G CP      
Sbjct: 199 GLRQWLARVIPASLKFATGAGIGLYLTIIGLSPSAGLGVIGHSSSDIVALGGCPPE---- 258

Query: 263 VAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGV-VGFVIIGYCLVKNIKGAMI 322
                              ++    C   +++S  +W+G+  G V+    ++   KGA++
Sbjct: 259 ------------------YLNADYSCNGHQLQSGRMWVGIFCGGVLTAILMMYKFKGAVL 318

Query: 323 YGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRDLGKAHFW 382
            GI  VT  SW R++ VT FP T  GD  +++FKKVV    I     A  +   G   F 
Sbjct: 319 AGIALVTITSWPRRSLVTMFPHTLTGDYNFDFFKKVVSFRKINRILVAQQWNVTG-GQFA 378

Query: 383 EAMVTFLYVDILDTTGTLYSMARFAGFVD-RNGDFEGQYFAFMSDATAIVVGSLLGTSPV 442
            A++TFLYVDI+D TGTLYSMA +AG VD R  DFEG   A++ DA +I +GSL G SPV
Sbjct: 379 IALITFLYVDIMDMTGTLYSMANYAGLVDPRTQDFEGSAVAYIVDALSISIGSLFGCSPV 438

Query: 443 TAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGVLMMKAV 502
           TAFIES +GI  GGRTG+  + V   F ++ FF P+ +SIP WA G  L+LVG +MMK+ 
Sbjct: 439 TAFIESGSGISAGGRTGILGMVVGICFFISLFFAPIFSSIPVWATGSTLVLVGSMMMKST 492

Query: 503 VEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHA 537
             I WS +  +IPAF+T+ LMP TYSIAYGLI GI  Y +L++
Sbjct: 499 TLINWSYLGDSIPAFITIALMPFTYSIAYGLIAGIICYALLNS 492

BLAST of MS003360 vs. ExPASy Swiss-Prot
Match: Q57772 (Putative permease MJ0326 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0326 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 8.7e-57
Identity = 166/509 (32.61%), Postives = 253/509 (49.71%), Query Frame = 0

Query: 26  WIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTDSGGTCSYSDCVPLCSDPS 85
           ++ K F+  +  ++   E  AG  TF+TMAYI+ VN  IL+ +G                
Sbjct: 5   FVEKYFEFEKYGTNLKVETLAGITTFMTMAYIIFVNPQILSTAG---------------- 64

Query: 86  VSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIVATIASSLIGCVIMGVFAN 145
                           D G                    ++VAT  +S I  ++MG++A 
Sbjct: 65  ---------------MDFGA-------------------VMVATCIASAIATLVMGLYAR 124

Query: 146 LPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGLIFLFISAIGFRAKLAKLV 205
            P ALAPGMG NAYF Y V    G G I +R AL AVFI G++F+ ++    R  +  ++
Sbjct: 125 YPFALAPGMGLNAYFTYGVC--LGMG-IDWRVALGAVFISGVLFIILTLTKIRTWIFNVI 184

Query: 206 PRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGACPESARVSVAPVVTSPNG 265
           P  ++  ++ GIGLF+AFIGL+S+   G+I  S +TLVT+                    
Sbjct: 185 PNAIKYGTAVGIGLFIAFIGLKSA---GIIVSSKATLVTL-------------------- 244

Query: 266 TVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVKNIKGAMIYGIVFVTAVSW 325
                             G +  P+  L + G  +    + +N+ GA++ GI+  + +  
Sbjct: 245 ------------------GNLMEPSTLLALFGIFLTSILVSRNVIGAILIGIIVTSLIGM 304

Query: 326 FRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRDLGKAHFWEAMVTFLYVDI 385
                ++ FP     +  +     +          GAL+   L        ++ F +VD+
Sbjct: 305 I--LGISPFP-----EGIFSMPPSIAPTFLQLDIMGALNLGLL------TIVLAFFFVDM 364

Query: 386 LDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSLLGTSPVTAFIESSTGIRE 445
            DT GTL ++A  AG++D++G       A M+DAT  VVGSLLGTS VT +IES++GI  
Sbjct: 365 FDTLGTLSALASQAGYLDKDGKLPRVEKALMADATGTVVGSLLGTSTVTTYIESASGIAL 406

Query: 446 GGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGVLMMKAVVEIEWSDMRQAI 505
           GGRTG  ++ VA  FLL+ FF P++ +IP +A    L++VG LMM++V  I++ D  +AI
Sbjct: 425 GGRTGFVSVVVAMLFLLSLFFYPVVKAIPPYATAAALVIVGALMMRSVKYIDFDDYTEAI 406

Query: 506 PAFMTLILMPLTYSIAYGLIGGIGTYVVL 535
           PAF+TL+ +PLT+SIA GL  G  TY +L
Sbjct: 485 PAFITLLTIPLTFSIATGLALGFITYPIL 406

BLAST of MS003360 vs. ExPASy TrEMBL
Match: A0A6J1JR25 (adenine/guanine permease AZG1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487572 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 488/569 (85.76%), Postives = 524/569 (92.09%), Query Frame = 0

Query: 8   TSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTD 67
           T+  P R+ RLN A AKTWIGKRFKL ERNS+FTTELRAGT TFLTMAYILAVNASIL D
Sbjct: 5   TTAQPGRLDRLNSAVAKTWIGKRFKLTERNSNFTTELRAGTTTFLTMAYILAVNASILAD 64

Query: 68  SGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIV 127
           SG TCS SDCVPLCSDPS+S+PNCSGSN+R+IQPD  CMFDPVNPGYA CLD +RRDLIV
Sbjct: 65  SGATCSQSDCVPLCSDPSISIPNCSGSNLRLIQPDRSCMFDPVNPGYAACLDQVRRDLIV 124

Query: 128 ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGL 187
           ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALTAV IEGL
Sbjct: 125 ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALTAVLIEGL 184

Query: 188 IFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGA 247
           IFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVT+G 
Sbjct: 185 IFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTLGG 244

Query: 248 CPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVK 307
           CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI  YC+VK
Sbjct: 245 CPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVITAYCMVK 304

Query: 308 NIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRD 367
           N+KGA IYGIVFVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTAGALSF+D
Sbjct: 305 NVKGATIYGIVFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTAGALSFKD 364

Query: 368 LGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSL 427
           LGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDATAIVVGSL
Sbjct: 365 LGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDATAIVVGSL 424

Query: 428 LGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGV 487
           LGTSPVTAFIESSTGIREGGRTGLT LTVA YF +AFFFTPLLASIP+WAVGPPLILVGV
Sbjct: 425 LGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFMAFFFTPLLASIPAWAVGPPLILVGV 484

Query: 488 LMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWSLAGFRKC 547
           LMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW  AG  KC
Sbjct: 485 LMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWGWAGLEKC 544

Query: 548 GLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
           GL+K ++ V  S+SNGQ  AEEDPA K++
Sbjct: 545 GLVKLRSHV--SASNGQLLAEEDPAPKSV 571

BLAST of MS003360 vs. ExPASy TrEMBL
Match: A0A6J1JSZ8 (adenine/guanine permease AZG1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487572 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 488/569 (85.76%), Postives = 524/569 (92.09%), Query Frame = 0

Query: 8   TSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTD 67
           T+  P R+ RLN A AKTWIGKRFKL ERNS+FTTELRAGT TFLTMAYILAVNASIL D
Sbjct: 5   TTAQPGRLDRLNSAVAKTWIGKRFKLTERNSNFTTELRAGTTTFLTMAYILAVNASILAD 64

Query: 68  SGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIV 127
           SG TCS SDCVPLCSDPS+S+PNCSGSN+R+IQPD  CMFDPVNPGYA CLD +RRDLIV
Sbjct: 65  SGATCSQSDCVPLCSDPSISIPNCSGSNLRLIQPDRSCMFDPVNPGYAACLDQVRRDLIV 124

Query: 128 ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGL 187
           ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALTAV IEGL
Sbjct: 125 ATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALTAVLIEGL 184

Query: 188 IFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGA 247
           IFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVT+G 
Sbjct: 185 IFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTLGG 244

Query: 248 CPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVK 307
           CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI  YC+VK
Sbjct: 245 CPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVITAYCMVK 304

Query: 308 NIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRD 367
           N+KGA IYGIVFVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTAGALSF+D
Sbjct: 305 NVKGATIYGIVFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTAGALSFKD 364

Query: 368 LGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSL 427
           LGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDATAIVVGSL
Sbjct: 365 LGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDATAIVVGSL 424

Query: 428 LGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGV 487
           LGTSPVTAFIESSTGIREGGRTGLT LTVA YF +AFFFTPLLASIP+WAVGPPLILVGV
Sbjct: 425 LGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFMAFFFTPLLASIPAWAVGPPLILVGV 484

Query: 488 LMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWSLAGFRKC 547
           LMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW  AG  KC
Sbjct: 485 LMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWGWAGLEKC 544

Query: 548 GLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
           GL+K ++ V  S+SNGQ  AEEDPA K++
Sbjct: 545 GLVKLRSHV--SASNGQLLAEEDPAPKSV 571

BLAST of MS003360 vs. ExPASy TrEMBL
Match: A0A6J1HLG6 (adenine/guanine permease AZG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464636 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 488/576 (84.72%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL+ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLSERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSG N+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGPNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. ExPASy TrEMBL
Match: A0A6J1HNU2 (adenine/guanine permease AZG1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464636 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 488/576 (84.72%), Postives = 529/576 (91.84%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           ME++   T+  P R+ RLN A AKTWIG+RFKL+ERNS+FTTELRAGT TFLTMAYILAV
Sbjct: 1   MEVQ---TTAQPGRLDRLNSAVAKTWIGRRFKLSERNSNFTTELRAGTTTFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+S+PNCSG N+R+IQPD  CMFDPVNPGYA CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSISIPNCSGPNLRLIQPDGSCMFDPVNPGYAACLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AV +EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS
Sbjct: 181 AVLMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVT+G CPE++RVSV+PVV+S NGTVSLM GGT SGGILC +GRMESPT+WLG+VGFVI
Sbjct: 241 TLVTLGGCPEASRVSVSPVVSSGNGTVSLMAGGTASGGILCLNGRMESPTMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
             YC+VKN+KGA IYGI+FVT VSW RKTSVTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 TAYCMVKNVKGATIYGIIFVTVVSWIRKTSVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK HFWEAMVTFLYVDILDTTGTLYSMARFAGFVD+NGDFEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDQNGDFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLT LTVA YF LAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTGLTVAAYFFLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVEIEW+DM+QAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLH  DW 
Sbjct: 481 PLILVGVLMMKAVVEIEWNDMQQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHVPDWG 540

Query: 541 LAGFRKCGLLKKKTEVSGSSSNGQPQAEEDPARKTL 577
            AG  KCGL+K ++ V  S+SNGQ  AEEDPARK++
Sbjct: 541 WAGLEKCGLVKLRSHV--SASNGQLLAEEDPARKSV 571

BLAST of MS003360 vs. ExPASy TrEMBL
Match: A0A0A0K8Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014490 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.7e-269
Identity = 479/579 (82.73%), Postives = 526/579 (90.85%), Query Frame = 0

Query: 1   MEIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAV 60
           MEIE   T+  P R++RLN A A+TWIGKRFKL ERNS+FTTELRAGTATFLTMAYILAV
Sbjct: 1   MEIE---TTSQPGRLNRLNSAVAQTWIGKRFKLTERNSNFTTELRAGTATFLTMAYILAV 60

Query: 61  NASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDG 120
           NASIL DSG TCS SDCVPLCSDPS+ + +C+GS++R+IQPD  CMFDPVNPGY +CLD 
Sbjct: 61  NASILADSGATCSQSDCVPLCSDPSIPLSSCTGSDLRIIQPDGSCMFDPVNPGYVDCLDQ 120

Query: 121 IRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALT 180
           +RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSG+ISY+SALT
Sbjct: 121 VRRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGSISYQSALT 180

Query: 181 AVFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPS 240
           AVF+EGLIFL ISAIGFRAKLAKL+P+PVRISSSAGIGLFLAFIGLQ+SQGIGLI F+PS
Sbjct: 181 AVFMEGLIFLLISAIGFRAKLAKLIPKPVRISSSAGIGLFLAFIGLQNSQGIGLIAFNPS 240

Query: 241 TLVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVI 300
           TLVTIG CPES+RVSVAPVV+  NGT+S+MTGGT S GILC +GRMESP +WLG+VGFVI
Sbjct: 241 TLVTIGGCPESSRVSVAPVVSYLNGTMSVMTGGTASDGILCLNGRMESPRMWLGIVGFVI 300

Query: 301 IGYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTA 360
           I YCLVKN+KGAMIYGI+FVTAVSW  +T VTAFP TA+G+AAY+YFKKVVDIHTIKSTA
Sbjct: 301 IAYCLVKNVKGAMIYGIIFVTAVSWIPRTRVTAFPETASGEAAYKYFKKVVDIHTIKSTA 360

Query: 361 GALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDAT 420
           GALSF+DLGK +FWEAM+TFLYVDILDTTGTLYSMARFAGF+D NG+FEGQYFAFMSDAT
Sbjct: 361 GALSFKDLGKPYFWEAMITFLYVDILDTTGTLYSMARFAGFIDENGNFEGQYFAFMSDAT 420

Query: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGP 480
           AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTV  YFLLAFFFTPLLASIP+WAVGP
Sbjct: 421 AIVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVGAYFLLAFFFTPLLASIPAWAVGP 480

Query: 481 PLILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWS 540
           PLILVGVLMMKAVVE+EW+DMRQAIPAF+TLILMPLTYSIAYGLIGGIGTYVVLH WDW+
Sbjct: 481 PLILVGVLMMKAVVEVEWNDMRQAIPAFITLILMPLTYSIAYGLIGGIGTYVVLHVWDWT 540

Query: 541 LAGFRKCGLLKKK---TEVSGSSSNGQPQAEEDPARKTL 577
           LA  +KCGLLK K    EVSG  SNGQ      P+ K+L
Sbjct: 541 LAALQKCGLLKSKLKDCEVSG--SNGQLVLHHHPSPKSL 574

BLAST of MS003360 vs. TAIR 10
Match: AT3G10960.1 (AZA-guanine resistant1 )

HSP 1 Score: 867.8 bits (2241), Expect = 4.8e-252
Identity = 435/568 (76.58%), Postives = 498/568 (87.68%), Query Frame = 0

Query: 2   EIEPNTTSHHPDRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVN 61
           ++   TT   P  ++RLN     + +GKRFKLAERNS+FTTELRAGTATFLTMAYILAVN
Sbjct: 8   QLPSTTTRPKPKLLNRLNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVN 67

Query: 62  ASILTDSGGTCSYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGI 121
           ASIL+DSGGTCS SDC+PLCS+P++    C+G  +R+IQPD  C F+PVNPGYA C++ I
Sbjct: 68  ASILSDSGGTCSVSDCIPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEI 127

Query: 122 RRDLIVATIASSLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTA 181
           R+DLIVAT+A+SLIGCVIMG+ ANLPLALAPGMGTNAYFAYTVVGFHGSG+ISYR+AL A
Sbjct: 128 RKDLIVATVAASLIGCVIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAA 187

Query: 182 VFIEGLIFLFISAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPST 241
           VFIEGLIFLFISAIGFRAKLAKLVP+PVRISSSAGIGLFLAFIGLQ++QGIGL+G+SPST
Sbjct: 188 VFIEGLIFLFISAIGFRAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPST 247

Query: 242 LVTIGACPESARVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVII 301
           LVT+ ACP S+R+S+APV+TS NGTVSL+ GG+VSG I+C  GRMESPT WLG+VGFVII
Sbjct: 248 LVTLAACPASSRISLAPVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVII 307

Query: 302 GYCLVKNIKGAMIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAG 361
            YCLVKN+KGAMIYGIVFVTAVSWFR T VTAFP T+AGDAA++YFKK+VD+H IK TAG
Sbjct: 308 AYCLVKNVKGAMIYGIVFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAG 367

Query: 362 ALSFRDLGKAHFWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATA 421
           ALSF  + K HFWEA+VTFLYVDILDTTGTLYSMARFAGFVD  GDF GQYFAFMSDA+A
Sbjct: 368 ALSFSGINKGHFWEALVTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASA 427

Query: 422 IVVGSLLGTSPVTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPP 481
           IV+GSLLGTSPVT FIESSTGIREGGRTGLTA+TVA YFLLA FFTPLLASIP+WAVGPP
Sbjct: 428 IVIGSLLGTSPVTVFIESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPP 487

Query: 482 LILVGVLMMKAVVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWDWSL 541
           LILVGV+MMK+V EI+W DMR+AIPAF+T+ILMPLTYS+AYGLIGGIG+YVVLH WDW  
Sbjct: 488 LILVGVMMMKSVTEIDWEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGE 547

Query: 542 AGFRKCGLLKKKTEVSGSSSNGQPQAEE 570
            G  K G LK+K +    ++NG  +A E
Sbjct: 548 EGLVKLGFLKRKVK-EEDNNNGVVKASE 574

BLAST of MS003360 vs. TAIR 10
Match: AT5G50300.1 (Xanthine/uracil permease family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.5e-146
Identity = 268/526 (50.95%), Postives = 355/526 (67.49%), Query Frame = 0

Query: 13  DRISRLNDAAAKTWIGKRFKLAERNSSFTTELRAGTATFLTMAYILAVNASILTDSGGTC 72
           D    LND  +K++IG+ FKL  R ++FTTELRA TATFLTMAYI+ VNA+IL DSG TC
Sbjct: 13  DMKKHLNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSGATC 72

Query: 73  SYSDCVPLCSDPSVSVPNCSGSNIRVIQPDAGCMFDPVNPGYANCLDGIRRDLIVATIAS 132
           S +DC  + S                  P   C+    NPGY  C+  +++DL+VAT  S
Sbjct: 73  SINDCSTVASSS---------------PPGPECVLGS-NPGYEQCISRVKKDLVVATSLS 132

Query: 133 SLIGCVIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSGNISYRSALTAVFIEGLIFLFI 192
           +++G + MG+ ANLP  LAPGMG NAY AY VVGF GSG+ISY +A+  V +EG  FL +
Sbjct: 133 AMVGSLAMGLLANLPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAV 192

Query: 193 SAIGFRAKLAKLVPRPVRISSSAGIGLFLAFIGLQSSQGIGLIGFSPSTLVTIGACPESA 252
           SA+G R KLA+L+P+ VR++ + GIG+F+AF+GLQ +QGIGL+G   STLVT+ AC E+ 
Sbjct: 193 SALGLRGKLARLIPQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTACAETD 252

Query: 253 RVSVAPVVTSPNGTVSLMTGGTVSGGILCTSGRMESPTLWLGVVGFVIIGYCLVKNIKGA 312
            V+ A                       C  G+M+SPT WL VVGF+I  + L+KN+KG+
Sbjct: 253 PVTGA-----------------------CLGGKMKSPTFWLAVVGFLITSFGLMKNVKGS 312

Query: 313 MIYGIVFVTAVSWFRKTSVTAFPATAAGDAAYEYFKKVVDIHTIKSTAGALSFRDLGKAH 372
           MIYGIVFVTA+SW R T VT FP T  GD+ Y YF K+VD H I+ST GA+SF +  K+ 
Sbjct: 313 MIYGIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFTKIVDFHKIQSTLGAISFTEFRKSE 372

Query: 373 FWEAMVTFLYVDILDTTGTLYSMARFAGFVDRNGDFEGQYFAFMSDATAIVVGSLLGTSP 432
            W A  T  YVD+L TTG LY+MA   GFV+ +G FEG+Y A++ DA + VVGS LG + 
Sbjct: 373 VWVAFATLFYVDLLGTTGVLYTMAEIGGFVE-DGKFEGEYAAYLVDAGSSVVGSALGVTT 432

Query: 433 VTAFIESSTGIREGGRTGLTALTVAGYFLLAFFFTPLLASIPSWAVGPPLILVGVLMMKA 492
              F+ESS G++EGG+TGLTA+ V  YFL + FFTPL+ ++P WAVGP L++VGV+MM  
Sbjct: 433 TATFVESSAGLKEGGKTGLTAVIVGLYFLASMFFTPLVTNVPRWAVGPSLVMVGVMMMGV 492

Query: 493 VVEIEWSDMRQAIPAFMTLILMPLTYSIAYGLIGGIGTYVVLHAWD 539
           V +I W + ++A+ AF+T++LMPLTYSIA G+I GIG Y+ L  +D
Sbjct: 493 VKDIRWGETKEAVTAFVTILLMPLTYSIANGIIAGIGIYLALSMYD 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6602590.11.3e-27584.90Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. sororia] >... [more]
XP_023553604.11.7e-27584.90adenine/guanine permease AZG1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023553610.11.7e-27584.90adenine/guanine permease AZG1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022964638.14.9e-27584.72adenine/guanine permease AZG1-like isoform X1 [Cucurbita moschata][more]
XP_022964639.14.9e-27584.72adenine/guanine permease AZG1-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SRK76.7e-25176.58Adenine/guanine permease AZG1 OS=Arabidopsis thaliana OX=3702 GN=AZG1 PE=2 SV=1[more]
Q84MA85.0e-14550.95Adenine/guanine permease AZG2 OS=Arabidopsis thaliana OX=3702 GN=AZG2 PE=2 SV=1[more]
L7WRR43.1e-10742.36Efflux pump notK' OS=Aspergillus versicolor OX=46472 GN=notK' PE=1 SV=1[more]
O943001.9e-10442.83Putative xanthine/uracil permease C887.17 OS=Schizosaccharomyces pombe (strain 9... [more]
Q577728.7e-5732.61Putative permease MJ0326 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / D... [more]
Match NameE-valueIdentityDescription
A0A6J1JR252.4e-27585.76adenine/guanine permease AZG1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JSZ82.4e-27585.76adenine/guanine permease AZG1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HLG62.4e-27584.72adenine/guanine permease AZG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1HNU22.4e-27584.72adenine/guanine permease AZG1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0K8Q29.7e-26982.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014490 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10960.14.8e-25276.58AZA-guanine resistant1 [more]
AT5G50300.13.5e-14650.95Xanthine/uracil permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006043Nucleobase cation symporter 2 familyPFAMPF00860Xan_ur_permeasecoord: 122..235
e-value: 1.6E-11
score: 43.6
coord: 291..523
e-value: 1.1E-15
score: 57.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 555..580
NoneNo IPR availablePANTHERPTHR43337:SF12ADENINE/GUANINE PERMEASE AZG1coord: 7..563
IPR045018Azaguanine-like transportersPANTHERPTHR43337XANTHINE/URACIL PERMEASE C887.17-RELATEDcoord: 7..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003360.1MS003360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0015205 nucleobase transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity