Homology
BLAST of MS003347 vs. NCBI nr
Match:
XP_022134046.1 (E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiquitin-protein ligase SHPRH [Momordica charantia])
HSP 1 Score: 3293.8 bits (8539), Expect = 0.0e+00
Identity = 1659/1675 (99.04%), Postives = 1664/1675 (99.34%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQSRPHRSGGIRLEIHANPETQVDI+VVLET+KSSDIKLNEINEPFYVEVDPTGWK
Sbjct: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI
Sbjct: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS
Sbjct: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN
Sbjct: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG
Sbjct: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVL+PIDTEGGNCIKKKRNKKNRVNV
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG
Sbjct: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN
Sbjct: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
Query: 961 DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLNKVWWLEAV 1020
DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVC + E+LNKVWWLEAV
Sbjct: 961 DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSEL----RERESLNKVWWLEAV 1020
Query: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV
Sbjct: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
Query: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV
Sbjct: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
Query: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHP 1200
QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMV KHP
Sbjct: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVCKHP 1200
Query: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI
Sbjct: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
Query: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS
Sbjct: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
Query: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI
Sbjct: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
Query: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH
Sbjct: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
Query: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM
Sbjct: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
Query: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL
Sbjct: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
Query: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN
Sbjct: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
Query: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1676
QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA
Sbjct: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1671
BLAST of MS003347 vs. NCBI nr
Match:
XP_038884903.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida])
HSP 1 Score: 2867.0 bits (7431), Expect = 0.0e+00
Identity = 1462/1698 (86.10%), Postives = 1551/1698 (91.34%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E +P+TQ+DIN +E SSDIK+ E+NEP +VEVD TGW
Sbjct: 1 MGRRKQRQPHRSGGIRFEDRGDPKTQIDINGAVE---SSDIKVAEVNEPIFVEVDRTGWN 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEV LMD+R EH FV F+ D SF E+SRYSLRFRLCNVN LL RIKFGHWP+
Sbjct: 61 SNEHHDISEVFLMDVRLEHPFVDFRLDKSFCENSRYSLRFRLCNVNGSLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS+DI LELI++ MEE ACSVVLSGNFDGPD+AISGLVHLANLKF TLRPV G ++S
Sbjct: 121 LSSNDILLELIERDMEEDMKACSVVLSGNFDGPDEAISGLVHLANLKFATLRPVEGVSVS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++TGSLRLRVE+LSSA ACESIFDNGRQLWKKSMM+TIAWLRPEV SE KYG+VKSTN
Sbjct: 181 QNTGSLRLRVEILSSAFAACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+D+ H GD +SN+RKH+NFDTV FYDAIKPSKD+PMLDEDIPNLLPKLR YQRRAAY
Sbjct: 241 MDTSLHHGTGDDTSNSRKHVNFDTVAFYDAIKPSKDEPMLDEDIPNLLPKLRQYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FSGN GVGENVQLISPLC+AL+C+DTC+R+FYNPFSGNLTL+AE+TLPHVYG
Sbjct: 301 WMVQREKGFSGNLGVGENVQLISPLCMALKCVDTCSRVFYNPFSGNLTLNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSH+MSVFEG KGF +E QQ V QRTEFKRLKRERVEC+C
Sbjct: 361 GILADEMGLGKTIELLACILSHQMSVFEGGKGFHDELQQPVEDQRTEFKRLKRERVECLC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGR+LKPIDTEGGNC K+KRN +N +NV
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRILKPIDTEGGNCRKEKRNNRNTLNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
+VR +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHT PGSIKLLVYEG
Sbjct: 481 IVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSL STLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLLSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK+KV DC+SSD PSD+LI H DAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKKKVPDCISSDIPSDLLINHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAE NNEDFRLDPLLSIHIHHNLAEILP AVDQSQS LK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVDQSQSRLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDN--HTMKKQKVSETSYAPCSD--TEKILDNPL------ 960
DQ RT EVKA++MDDSE YDN H MKKQKVSET YAPCS+ T K++D PL
Sbjct: 901 DQFCPRTSEVKAARMDDSEKYDNHVHVMKKQKVSETLYAPCSEDNTGKMIDRPLELTRKD 960
Query: 961 ---------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYV 1020
+PH SSSYF+D S RKACE MRQKYLAVFSSKLS+AQQEFTKS+MQV
Sbjct: 961 TNTKKEENCEPHLSSSYFDDISIRKACEAMRQKYLAVFSSKLSVAQQEFTKSYMQV---- 1020
Query: 1021 LLLYSRLE---NLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFR 1080
S L+ NLN VWWLEAV HAEQNKDF HELIRK+EEAVSGN+NNS+ SRVGSRFR
Sbjct: 1021 ---GSELKDRGNLNHVWWLEAVHHAEQNKDFSHELIRKVEEAVSGNLNNSK-SRVGSRFR 1080
Query: 1081 SINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPC 1140
SINALKYHVQ+GLDLLEASR VVLDRLLEIDQTMENPKEEDIERVRYC+NCQADSNGPPC
Sbjct: 1081 SINALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPC 1140
Query: 1141 VLCELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS 1200
VLCELD+LFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKN SSS
Sbjct: 1141 VLCELDDLFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSS 1200
Query: 1201 RVGYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK 1260
RVGYEE NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK
Sbjct: 1201 RVGYEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK 1260
Query: 1261 EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKF 1320
EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRD DDD SAYAISEHELPAASVQYSSDKF
Sbjct: 1261 EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRD-DDDSSAYAISEHELPAASVQYSSDKF 1320
Query: 1321 LSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEE 1380
+SLAML+RVKGKLRYLKGLVQSKQN PL SSSNLALTQ+PA MSTSMEQKSENT KADEE
Sbjct: 1321 MSLAMLARVKGKLRYLKGLVQSKQNIPLDSSSNLALTQQPATMSTSMEQKSENTSKADEE 1380
Query: 1381 SCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIA 1440
SCPVCQEKLSNQKMVFQCGH+ CCKCLFAMTEKTL SKIQT+WVMCPTCRQHTDFGNIA
Sbjct: 1381 SCPVCQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIA 1440
Query: 1441 FADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSS 1500
+ADDSKNE LDPSTLHETSREHE ITVQGSYGTK+EAVVRRILWIKYTDSKAKVLVFSS
Sbjct: 1441 YADDSKNETLDPSTLHETSREHELSITVQGSYGTKVEAVVRRILWIKYTDSKAKVLVFSS 1500
Query: 1501 WNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSA 1560
WNDVLDVLQYAFAAN ITFIRMKGGR+S TAISEFRGQKINAKEN KKR +VPEL+SA
Sbjct: 1501 WNDVLDVLQYAFAANSITFIRMKGGRQSHTAISEFRGQKINAKENQKKRPSGKVPELRSA 1560
Query: 1561 QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDT 1620
QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDT
Sbjct: 1561 QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQKNKTFVHRFIVKDT 1620
Query: 1621 VEESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHL 1675
VEESI+KLNRSRE+SSFITGN KNQDQP LTLKEVESLFASKASPL ++D+K SETLRHL
Sbjct: 1621 VEESIYKLNRSRESSSFITGNTKNQDQPLLTLKEVESLFASKASPLRENDEKGSETLRHL 1680
BLAST of MS003347 vs. NCBI nr
Match:
XP_023551402.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2863.2 bits (7421), Expect = 0.0e+00
Identity = 1445/1691 (85.45%), Postives = 1553/1691 (91.84%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E NP+TQVDI+ ++ET +SSDIKL+E+NEPF+VEVD TGW
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVNPKTQVDIHGIVETVESSDIKLDEVNEPFFVEVDRTGWH 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEVVLMD+R EH FVG + ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS++ISLELI++ +EE + C VVLSG+FDGPD+AISGLVHLANLKF+TLRPVMG + S
Sbjct: 121 LSSNEISLELIERRVEEDMNGCLVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V+STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+D+H E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+TLPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSH MSVFE KGF +E QQ+VG QRT+FKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHPMSVFEDGKGFDDEMQQLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGRVL+P+DT+ GNC K KRNK+N VNV
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRVLEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLS+TL VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL+GLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLFGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV VSSD PSDILITH DAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
DQ Y RTCEVKASKMDDSE DNH +KKQKV+ T Y PCSDTEKI+D+PL
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960
Query: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
+PHRSSSYFND+ RKACE MRQKYLAVFSSKLSIAQQEFTKS++QVC L
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCNE---LK 1020
Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080
Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQTGLDLLEASR +VL RLLEIDQTMENPK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1320
Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 ALSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
E LDPSTLH+TSRE E I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
LQYAFAAN ITF+RMKGGRKS TAISEFRGQKIN KENHKK+ A+VPEL+SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620
Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
LNRSRE+SSFITGN KNQDQP TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680
BLAST of MS003347 vs. NCBI nr
Match:
XP_022938761.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata])
HSP 1 Score: 2851.2 bits (7390), Expect = 0.0e+00
Identity = 1439/1691 (85.10%), Postives = 1549/1691 (91.60%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E +P+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS+DISLELI++ +EE + CSVVLSG+FDGPD+AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121 LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+D+H E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRMSVFE KG +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLS+T VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV VSSD PSDILITH DAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
DQ Y RTCEVKASKMDDSE DNH +KKQKV+ T YAPCSDTEKI+D+PL
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
+PHRSSSYFND+ RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC L
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020
Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080
Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320
Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
E LDPSTLH+TSRE E I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+ A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620
Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
LNRSRE+SSFITGN KNQDQP TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680
BLAST of MS003347 vs. NCBI nr
Match:
KAG6578487.1 (E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2845.8 bits (7376), Expect = 0.0e+00
Identity = 1436/1691 (84.92%), Postives = 1547/1691 (91.48%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E NP+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVNPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS+DISLELI++ +EE + CSVVLSG+FDGP +AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121 LSSNDISLELIERHVEEDMNGCSVVLSGSFDGPYEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVVSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+D+H E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSHR+SVFE KG +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRISVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLS+T VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV VSSD PSDILITH DAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
DQ Y RTCEVKASKMDDSE DNH +KKQKV+ T YAPCSDTEKI+D+PL
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
+PHRSSSYFND+ RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC L
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020
Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080
Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSSDKF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLATLS 1320
Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
E LDPSTLH+TSRE E I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+ A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620
Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
LNRSRE+SSFI GN KNQDQP TLKEVESLFAS+ SPL ++D+KESETLRHLAPS+AA
Sbjct: 1621 LNRSRESSSFIAGNTKNQDQPVFTLKEVESLFASRPSPLRENDEKESETLRHLAPSLAAT 1680
BLAST of MS003347 vs. ExPASy Swiss-Prot
Match:
Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)
HSP 1 Score: 523.1 bits (1346), Expect = 1.2e-146
Identity = 380/1240 (30.65%), Postives = 576/1240 (46.45%), Query Frame = 0
Query: 415 RVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNK 474
R EC+CG + + R VQC C WQHA CV Y K +KP
Sbjct: 658 RFECICGELDQIDRKPR--VQCLKCHLWQHAKCVNYDEKNLKIKPF-------------- 717
Query: 475 KNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIK 534
C CL M+ P++T ATLI+ P+ I QW EI RH R S++
Sbjct: 718 -------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLR 777
Query: 535 LLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEG--DRRFMRFQK 594
+LVY+GV+ +Q + L DI++ +YDVL+ +L++ H D R +R QK
Sbjct: 778 VLVYQGVKKDGF-----LQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQK 837
Query: 595 RYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLY 654
RY IP+PL + WWRICLDE QMVE A EMA RL +RWCI+GTP+QR LEDL+
Sbjct: 838 RYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLF 897
Query: 655 GLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPP 714
GL+ F+ P+ V WWV ++ PY +++P + + F +I+WRS K V D++Q+PP
Sbjct: 898 GLVVFLGIEPYCVKHWWVRLLYRPYCKKNP---QHLYSFIAKILWRSAKKDVIDQIQIPP 957
Query: 715 QEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILIT 774
Q E++ WL FSP+E HFY RQHE C ++V+ L RK++D + D
Sbjct: 958 QTEEIHWLHFSPVERHFYHRQHEVC---CQDVVVKL------RKISDWALKLSSLD---- 1017
Query: 775 HTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRS 834
+L LL+LRQACCHPQ LQ+S MTMEE+L L K E EEA R+
Sbjct: 1018 RRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQL 1077
Query: 835 VVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAV 894
V ALN LAGI II+ +++ A LY+E L +E + + D L +H HNL E+L
Sbjct: 1078 VCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL---- 1137
Query: 895 DQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKP 954
I +P P
Sbjct: 1138 ----------------------------------------------------IARHPGIP 1197
Query: 955 HRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLN 1014
D + + +R+ Y++ +++++ AQQ +Q + L R + N
Sbjct: 1198 ----PTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALYP--VQQTIHEL---QRKIHSN 1257
Query: 1015 KVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS-RVGSRFRSINALKYHVQTGL 1074
WWL + H EL++++ ++ N + +FR L++ + T +
Sbjct: 1258 SPWWLNVI-HRAIEFTIDEELVQRVRNEITSNYKQQTGKLSMSEKFRDCRGLQFLLTTQM 1317
Query: 1075 DLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYE 1134
+ L + +V + + ++ P IE C A CV C+ DELF EYE
Sbjct: 1318 EELNKCQKLVREAVKNLEGP---PSRNVIESATVCHLRPARLPLNCCVFCKADELFTEYE 1377
Query: 1135 ARLFRLNKVQGGMVSSVEEAVEAQK-----KKSALNRFYWSLLQQNKNLSSSRVGYEESN 1194
++LF N V+ G + EE +E ++ + R W++ + +++ + + + +S+
Sbjct: 1378 SKLFS-NTVK-GQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAI-LSFAKSH 1437
Query: 1195 KRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSL 1254
+ DV E + + + L E +KEY
Sbjct: 1438 RFDV------------------------------EFVDEGSTSMDLFEAWKKEYKLLHEY 1497
Query: 1255 AIAQAQVLNAHDEIKMATTRLCLRD----NDDDDSAYAISEHELPAASVQYSSDKFLSLA 1314
+A ++A DE+ MAT RL +RD + + I HE+ ++ +DK ++ +
Sbjct: 1498 WMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATS 1557
Query: 1315 MLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPV 1374
L + G+L YL L +S+ T G + E CP+
Sbjct: 1558 QLQKKLGQLLYLTNLEKSQDKTSGG---------------------------VNPEPCPI 1617
Query: 1375 CQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADD 1434
C +L Q V CGH C +C+ + E+ S + + C CRQ T I++
Sbjct: 1618 CARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSS--IKCAICRQTTSHKEISYVFT 1677
Query: 1435 SKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDV 1494
S+ E I V+GS+ TK+EAVVR ++ I+ D AK LVFS+W DV
Sbjct: 1678 SE------------KANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDV 1682
Query: 1495 LDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLL 1554
LD++ A N + F ++ + Q +S F K+ Q+ +LLL
Sbjct: 1738 LDIISKALTDNNMEFAQISRVKTFQENLSAF-----------KRDPQI------NILLLP 1682
Query: 1555 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIH 1614
+ G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ T VHRF++K T+EE +
Sbjct: 1798 LHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQ 1682
Query: 1615 KLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1643
+ ++ E S +AK+ + LT+ ++ LF + L
Sbjct: 1858 AMLKTAER-SHTNSSAKHSEASVLTVADLADLFTKETEEL 1682
HSP 2 Score: 80.9 bits (198), Expect = 1.5e-13
Identity = 56/172 (32.56%), Postives = 90/172 (52.33%), Query Frame = 0
Query: 280 LDEDIPNLLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMF 339
+D P L+P LRPYQR A WM+Q+E F + + + + E L +++
Sbjct: 293 VDVQHPALIPVLRPYQREAVNWMLQQE-CFRSSPATESALHFLWREIVTSEGL----KLY 352
Query: 340 YNPFSGNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSH--------RMSVFEGEK 399
YNP++G + + P + GGILADEMGLGKT+E+LA IL+H +++ EG+
Sbjct: 353 YNPYTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKV 412
Query: 400 GFGNESQQIVGG--QRTEFKRLK---RERVEC------VCGAVSENHRYKGL 433
GG ++TE + ++ +E+V+C + AV E + KG+
Sbjct: 413 VNYFIPSHYFGGKLKKTEIQNIEFEPKEKVQCPPTRVMILTAVKEMNGKKGV 459
BLAST of MS003347 vs. ExPASy Swiss-Prot
Match:
Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)
HSP 1 Score: 515.0 bits (1325), Expect = 3.2e-144
Identity = 375/1242 (30.19%), Postives = 572/1242 (46.05%), Query Frame = 0
Query: 415 RVECVCGAVSE-NHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRN 474
R EC+CG + H+ + VQC C WQHA CV Y K +KP
Sbjct: 649 RFECICGEFDQIGHKPR---VQCLKCHLWQHAKCVNYEEKNLKVKPF------------- 708
Query: 475 KKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSI 534
C CL M+ P++T ATLI+ P+ I QW EI RH R S+
Sbjct: 709 --------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSL 768
Query: 535 KLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEG--DRRFMRFQ 594
++LVY+GV+ +Q + L DI++ +YDVL+ +L++ + H D R +R Q
Sbjct: 769 RVLVYQGVKKHGF-----LQPHFLAEQDIVIITYDVLRSELNYVNIPHSNSEDGRRLRNQ 828
Query: 595 KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 654
KRY IP+PL + WWRICLDE QMVE A EMA RL +RWCI+GTP+QR LEDL
Sbjct: 829 KRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDL 888
Query: 655 YGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLP 714
+GL+ F+ P+ V WW+ ++ PY +++P + + F +IMWRS K V D++Q+P
Sbjct: 889 FGLVVFLGIEPYCVKHWWIRLLYHPYCKKNP---QHLYSFIAKIMWRSAKKDVIDQIQIP 948
Query: 715 PQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK-RKVADCVSSDAPSDIL 774
PQ E++ WL FSP+E HFY RQHE C +D VK RK++D + D
Sbjct: 949 PQTEEMHWLHFSPVERHFYHRQHEVC----------CQDAIVKLRKISDWALKLSSLD-- 1008
Query: 775 ITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALR 834
+L LL+LRQACCHPQ LQ+S MTMEE+L L K E EEA R
Sbjct: 1009 --RRTVSSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHR 1068
Query: 835 RSVVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPS 894
+ V ALN LAGI II+ +++ A LY+E L +E + + D L +H HNL E+L +
Sbjct: 1069 QLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLGA 1128
Query: 895 AVDQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL 954
K + T
Sbjct: 1129 ------------------------------------KHPGIPPT---------------- 1188
Query: 955 KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLEN 1014
D + + +R+ Y++ +++++ AQQ + ++S
Sbjct: 1189 --------LRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQSIRELQRKIHS---- 1248
Query: 1015 LNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS-RVGSRFRSINALKYHVQT 1074
N WWL + H EL++++ +S N + +FR L++ + T
Sbjct: 1249 -NSPWWLNVI-HRAMEFSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRDCRGLQFLLTT 1308
Query: 1075 GLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1134
++ L + +V + + ++++ P E IE C A CV C+ DELF E
Sbjct: 1309 QMEELHKFQKLVREAVKKLEKP---PSREVIESATVCHLRPARLPLNCCVFCKADELFTE 1368
Query: 1135 YEARLFRLNKVQGGMVSSVEEAVEAQK-----KKSALNRFYWSLLQQNKNLSSSRVGYEE 1194
YE++LF N V+ G + EE +E ++ + R W++ + +++ + + +
Sbjct: 1369 YESKLF-FNTVK-GQTAIFEEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAI-LSFAR 1428
Query: 1195 SNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHAR 1254
S++ DV E + + + L E +KEY
Sbjct: 1429 SHRFDV------------------------------EYVDEGSVSMDLFEAWKKEYKLLH 1488
Query: 1255 SLAIAQAQVLNAHDEIKMATTRLCLR----DNDDDDSAYAISEHELPAASVQYSSDKFLS 1314
+ ++A DE+ MAT RL +R + + I HE+ ++ +DK ++
Sbjct: 1489 EYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEVEQNRIKLVNDKAVA 1548
Query: 1315 LAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESC 1374
+ L + G+L YL L +S+ T G + E C
Sbjct: 1549 TSQLQKKLGQLLYLTNLEKSQDKTSGG---------------------------INPEPC 1608
Query: 1375 PVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFA 1434
P+C +L Q V CGH C +C + E+ S + + C CRQ T +++
Sbjct: 1609 PICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSS--IKCAICRQTTSHKEVSYV 1668
Query: 1435 DDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWN 1494
S+ E I V+GS+ TK+EAVVR ++ I+ D AK LVFS+W
Sbjct: 1669 FTSE------------KANQEDDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQ 1673
Query: 1495 DVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLL 1554
DVLD++ A N + F ++ + Q +S F+ P + +LL
Sbjct: 1729 DVLDIISKALTDNNMEFTQISRIKTFQENLSAFKYD--------------PHI---NILL 1673
Query: 1555 LLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEES 1614
L + G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ T VHRF++K T+EE
Sbjct: 1789 LPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEER 1673
Query: 1615 IHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1643
+ + ++ E S + + K+ + LT+ + LF + L
Sbjct: 1849 MQAMLKTAER-SHTSSSGKHSEASVLTVAGLADLFTKENEEL 1673
HSP 2 Score: 78.6 bits (192), Expect = 7.6e-13
Identity = 41/103 (39.81%), Postives = 61/103 (59.22%), Query Frame = 0
Query: 280 LDEDIPNLLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMF 339
+D P L+P LRPYQR A WM+Q+E +F ++ + + + L +++
Sbjct: 288 VDVQHPALIPVLRPYQREAVNWMLQQE-QFRSAPPADNSLHFLWREIVTPDGL----KLY 347
Query: 340 YNPFSGNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSH 383
YNP++G + P + GGILADEMGLGKT+E+LA IL+H
Sbjct: 348 YNPYTGCIIRDFPHAGPQLLGGILADEMGLGKTVEVLALILTH 385
BLAST of MS003347 vs. ExPASy Swiss-Prot
Match:
Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)
HSP 1 Score: 235.0 bits (598), Expect = 6.4e-60
Identity = 316/1368 (23.10%), Postives = 553/1368 (40.42%), Query Frame = 0
Query: 285 PNLLPKLRPYQRRAAYWMVQR--EKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNP 344
P L +L P+Q R WM +R EK + N + PL + L ++ N
Sbjct: 212 PRLSSELLPFQMRVLEWMKRREEEKFLTSND--------LPPLWYHCKSLFDDRMVYVNH 271
Query: 345 FSGNLTLSAEKTL----PHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQ 404
G +T S EKT + GGILADEMG+GKT+E+L +L H++ +
Sbjct: 272 VYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPI------------ 331
Query: 405 QIVGGQRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLK 464
+ D VG + K
Sbjct: 332 --------------------------------------SLTDTCTFDQVVGKNVK----- 391
Query: 465 PIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILF 524
+ ATLI+ P+ IL
Sbjct: 392 ---------------------------------------------YSKATLIITPSTILD 451
Query: 525 QWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDS 584
QW +EI H S+K+ Y+G+R ++ + + ++ DI++TSY L+ +L +
Sbjct: 452 QWLSEIDLHV--PSLKVFHYQGIRKSNGLKSAKI----FLDCDIVVTSYSDLRFELLYT- 511
Query: 585 DRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWC 644
E R +R +KR+ +PL + WWRIC+DE QMVE++ + +M R+ + W
Sbjct: 512 ---ESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWT 571
Query: 645 ITGTPIQRKLEDLYGLLRFVKASPFDVYR--WWVEVIRDPYERRDPGAMEFTHKFFKQIM 704
++GTP++ +++DL+GLL ++ SP +Y+ W+++I R EF F ++
Sbjct: 572 VSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVR------EFC-DLFGSLV 631
Query: 705 WRSLKIHVTDELQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRK 764
R K V +EL+LPPQ S +EE YQ +S A + + KD R
Sbjct: 632 CRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQ----DLLSEAAKSLHFFKD----RN 691
Query: 765 VADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM-TMEEILM 824
+ C D + L++LRQACCHPQVG + PM ++ ++L+
Sbjct: 692 LDLC--------------DEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLV 751
Query: 825 VLVSKTKIEGEEALRRSVVALNALAG-IAIIEKKFSEAVSLYKEA---LELAEGNNEDFR 884
++ +T +L R + + + G I K +++A++++ E +ELA E+
Sbjct: 752 FMLEQTN-STFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENV- 811
Query: 885 LDPLLSIHIHHNLAEILPSAVDQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKV 944
I+++ E + + L + R V K+ ++K +K
Sbjct: 812 --------IYNSKYEDHGKNLPINYFGLDHFIHLRVWYVLLHKIYFFIASAYFSLKNEKF 871
Query: 945 SETSYAPCSDTEKILDNPLKPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFT 1004
Y D + + + + S K E +K++ K+ Q+ +
Sbjct: 872 ENEFYLLAQDLRRKIMSDVIIKTS----------KHLEEFSEKFIPKKLVKIPRLQKSYA 931
Query: 1005 KSHMQVCFYVLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS 1064
K ++ + +E+ N++ + E D E F LI ++ + ++
Sbjct: 932 KG-------LITGHGIIEDYNRL-YKELNDQKEVLIKFRDRLIHLMKLPLLDQESDPTGD 991
Query: 1065 RVGSRFRSINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMEN--PKEEDIERVRYCQNC 1124
+ + + Y ++ R ++ DR+ + T+ E ++E+ + ++
Sbjct: 992 EYEESLNAQSEISY-------CIDVYRQMLSDRVAAVSGTINTFVSHETELEKYKLIESI 1051
Query: 1125 QADSNGPPCVLCELDE----LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFY 1184
+ E D+ F+E E + ++ G +++ V ++A + +
Sbjct: 1052 KKSEKSLDKQAEERDKKYLLYFEEREEARPKADQ-YGSLINIVSRLLDASNRST------ 1111
Query: 1185 WSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHPSELEVVL----GVIKNFCKTQLGKE 1244
S + +KN+ YE + + + K EL ++ I+ + + Q +
Sbjct: 1112 -SSFETSKNMEE----YERIDAMAKEQSRICQKLEKELSIIQLTYNSRIEYYKQLQEISD 1171
Query: 1245 SIAAAN-KQLHLLEYMRKEYGHARSL--AIAQAQVLNAHDEIKMATTRLCLRDNDDDDSA 1304
S+ + L Y++ + + L I +A V+ EI N +
Sbjct: 1172 SLMPPPVSNISLNNYVKDDEKKQKFLNSVIIKASVI-LEKEISEKQDEASQTTNVAELVN 1231
Query: 1305 YAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEP 1364
ISE +P G + L+ L + K NT + + +
Sbjct: 1232 QKISEMNIP---------------------GHIHLLRELEEEKSNTQRKIAHFESRRRYL 1291
Query: 1365 AIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKI 1424
+ + K+E+ + C +C++ + Q + CGH+ C CL E L+ S
Sbjct: 1292 TNLYEHIVLKAES-----HQICIICRD-IIKQGFITTCGHLYCSFCL----EAWLKHSS- 1324
Query: 1425 QTQWVMCPTCRQHTDFGNIAFADDSKN------------------EILDPSTLHETSREH 1484
CP C+ + N + +S++ EILD + S
Sbjct: 1352 -----SCPMCKTKLNKNNAYYIGESRDIYSRQEFVTGFNKRDERLEILDDEAYRQISN-- 1324
Query: 1485 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1544
+ ++ S+G+KI+ + + +L++K+ + KV+VFS W DVLDVL +F ANGI FIR
Sbjct: 1412 ---MELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRF 1324
Query: 1545 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1604
G K+ T + F+ E +S QVL L + ++GL L A HV +
Sbjct: 1472 DGKSKN-TCLKRFK-----------------EERSLQVLTLHARSQSSGLTLTNATHVFM 1324
Query: 1605 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSR 1609
EPLLN E QAISRVHRIGQ TFV+ +IV+DTVE I L+ ++
Sbjct: 1532 CEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVEDTVEGHILNLSLTK 1324
BLAST of MS003347 vs. ExPASy Swiss-Prot
Match:
Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)
HSP 1 Score: 172.6 bits (436), Expect = 3.9e-41
Identity = 142/525 (27.05%), Postives = 213/525 (40.57%), Query Frame = 0
Query: 287 LLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRM--FYNPFS 346
LL +LRPYQ++A +WM Q EK GN E ++ P C CL + + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEK---GNC-TDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403
Query: 347 GNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSV---------FEGEKGFGN 406
G+ T+ TL GGILAD MGLGKT+ ++ +L+H +EG+K +
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISS 463
Query: 407 ESQQIVGG--QRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPK 466
+ + T+F +G+ +
Sbjct: 464 SVDDLTSPPVKATKF---------------------------------------LGFDKR 523
Query: 467 GRVLKPIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCP 526
K + GGN LIVCP
Sbjct: 524 LLEQKSVLQNGGN------------------------------------------LIVCP 583
Query: 527 APILFQWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKED 586
+L QW EI H +PGS+ + V+ G + LS SD+++T+Y VL +
Sbjct: 584 MTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSE 643
Query: 587 LSHD-SDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLY 646
S + S HEG + + W+RI LDE ++++ + + A+ L
Sbjct: 644 FSQENSADHEG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALV 703
Query: 647 ASHRWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFF 706
A RWC+TGTPIQ LEDLY LLRF++ P+ + WW ++++ P+E D ++
Sbjct: 704 ADRRWCLTGTPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSIL 736
Query: 707 KQIMWRSLKIHVTDE----LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGL 766
K IM R K E L LPP + +V + S E FY + R ++
Sbjct: 764 KPIMLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK-- 736
Query: 767 KDDFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
D FV++ + H A +L LL+LRQ C HP
Sbjct: 824 FDQFVEQG-------------KVLHNYA-SILELLLRLRQCCDHP 736
HSP 2 Score: 107.5 bits (267), Expect = 1.5e-21
Identity = 82/311 (26.37%), Postives = 145/311 (46.62%), Query Frame = 0
Query: 1327 GSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLF 1386
G SS L + +++ E K ++ CP+C E L + ++ C H +C +CL
Sbjct: 761 GKSSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLL 820
Query: 1387 AMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREHETFITV 1446
A + +CP CR SK E++ T E + V
Sbjct: 821 ASWRNSTSG--------LCPVCRNTV----------SKQELITAPT------ESRFQVDV 880
Query: 1447 QGSY--GTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGR 1506
+ ++ +KI A++ + ++ + SK+ ++FS W LD+LQ + N +F+R+ G
Sbjct: 881 EKNWVESSKITALLEELEGLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG-- 940
Query: 1507 KSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1566
++ ++ K + E S VLL+ ++ G G+NL A + +++P
Sbjct: 941 ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1000
Query: 1567 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKNQDQP 1626
NPA E QA+ R+HRIGQ + + RFIVK TVEE + + ++ I+G +Q+
Sbjct: 1001 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQR--MISGALTDQEVR 1028
Query: 1627 FLTLKEVESLF 1636
++E++ LF
Sbjct: 1061 SARIEELKMLF 1028
BLAST of MS003347 vs. ExPASy Swiss-Prot
Match:
Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)
HSP 1 Score: 163.3 bits (412), Expect = 2.4e-38
Identity = 144/523 (27.53%), Postives = 208/523 (39.77%), Query Frame = 0
Query: 291 LRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALE-CLDTCTRMFYNPFSGNLTL 350
LRPYQ++A YWM + EK V + + + P A C + ++ N FSG T+
Sbjct: 616 LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675
Query: 351 SAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFK 410
GGILAD MGLGKT+ +A IL+ G GN +
Sbjct: 676 QFPTATQMARGGILADAMGLGKTVMTIALILA--------RPGRGNPENE---------- 735
Query: 411 RLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIK 470
D+ A +AD
Sbjct: 736 ---------------------------DVLVADVNAD----------------------- 795
Query: 471 KKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTR 530
KRN+K E+ A T A TLI+CP +L QW E+ H++
Sbjct: 796 -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855
Query: 531 PGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMR 590
P ++ +LVY G T + ++ + D++LT+Y VL D
Sbjct: 856 PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915
Query: 591 FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
+ + RI W+RI LDE ++S T A + L + RWC+TGTP+Q KLE
Sbjct: 916 ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975
Query: 651 DLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 710
DLY LL F+ P+ + WW ++I+ PYE DP ++ + +M R K E
Sbjct: 976 DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000
Query: 711 --LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDA 770
L+LPP + QV S E FY + R +Q D FV A
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFV-----------A 1000
Query: 771 PSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 809
+L + + +L LL+LRQ C HP + S S Q + +
Sbjct: 1096 QGKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000
HSP 2 Score: 106.3 bits (264), Expect = 3.4e-21
Identity = 87/322 (27.02%), Postives = 141/322 (43.79%), Query Frame = 0
Query: 1320 SKQNTPLGSSSNLALTQEPAIMSTS------MEQKSENTPKADEESCPVCQEKLSNQKMV 1379
S+Q L S + L P +S + +E+ ++ + + CP+C E ++ ++
Sbjct: 994 SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053
Query: 1380 FQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTL 1439
C H +C +CL S +CP CR + E++
Sbjct: 1054 TPCAHRMCRECLLT--------SWRSPSCGLCPICRTIL----------KRTELIS---- 1113
Query: 1440 HETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 1499
T + +K+ +++ + IK + S K +VFS W LD+L+
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173
Query: 1500 GITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLL 1559
G F+R G K+ K K + E K +LL+ ++ G GLNL
Sbjct: 1174 GFEFLRFDG--------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLT 1233
Query: 1560 EAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSF 1619
A V L++P NPA E QAI R+HRIGQ+ FV RFIVKDTVEE + ++ ++
Sbjct: 1234 AASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQR--M 1276
Query: 1620 ITGNAKNQDQPFLTLKEVESLF 1636
I G +++ L+E++ LF
Sbjct: 1294 IAGALTDEEVRSARLEELKMLF 1276
BLAST of MS003347 vs. ExPASy TrEMBL
Match:
A0A6J1BYG6 (E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415 PE=3 SV=1)
HSP 1 Score: 3293.8 bits (8539), Expect = 0.0e+00
Identity = 1659/1675 (99.04%), Postives = 1664/1675 (99.34%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQSRPHRSGGIRLEIHANPETQVDI+VVLET+KSSDIKLNEINEPFYVEVDPTGWK
Sbjct: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI
Sbjct: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS
Sbjct: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN
Sbjct: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG
Sbjct: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVL+PIDTEGGNCIKKKRNKKNRVNV
Sbjct: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG
Sbjct: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL
Sbjct: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA SLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK
Sbjct: 841 GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN
Sbjct: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
Query: 961 DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLNKVWWLEAV 1020
DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVC + E+LNKVWWLEAV
Sbjct: 961 DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSEL----RERESLNKVWWLEAV 1020
Query: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV
Sbjct: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
Query: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV
Sbjct: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
Query: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHP 1200
QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMV KHP
Sbjct: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVCKHP 1200
Query: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI
Sbjct: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
Query: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS
Sbjct: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
Query: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI
Sbjct: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
Query: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH
Sbjct: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
Query: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM
Sbjct: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
Query: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL
Sbjct: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
Query: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN
Sbjct: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
Query: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1676
QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA
Sbjct: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1671
BLAST of MS003347 vs. ExPASy TrEMBL
Match:
A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)
HSP 1 Score: 2851.2 bits (7390), Expect = 0.0e+00
Identity = 1439/1691 (85.10%), Postives = 1549/1691 (91.60%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E +P+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW
Sbjct: 1 MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS+DISLELI++ +EE + CSVVLSG+FDGPD+AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121 LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+D+H E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSHRMSVFE KG +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLS+T VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV VSSD PSDILITH DAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
DQ Y RTCEVKASKMDDSE DNH +KKQKV+ T YAPCSDTEKI+D+PL
Sbjct: 901 DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960
Query: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
+PHRSSSYFND+ RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC L
Sbjct: 961 KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020
Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080
Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320
Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
E LDPSTLH+TSRE E I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+ A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620
Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
LNRSRE+SSFITGN KNQDQP TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680
BLAST of MS003347 vs. ExPASy TrEMBL
Match:
A0A6J1JXA7 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489159 PE=3 SV=1)
HSP 1 Score: 2834.7 bits (7347), Expect = 0.0e+00
Identity = 1432/1692 (84.63%), Postives = 1545/1692 (91.31%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQ +PHRSGGIR E NP+T+VD N ++ET +SSDIKL+E EPF+VEVD TGW
Sbjct: 1 MGRRKQKQPHRSGGIRFEDRVNPKTEVDGNGIVETVESSDIKLDEGKEPFFVEVDRTGWH 60
Query: 61 SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
SN+H DISEVVLMD+R EH FVG + ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61 SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120
Query: 121 LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
LSS++ISLELI++ +EE + CSVVLSG+FDGPD+AISGLVHLANLKF+TLRPVMG + S
Sbjct: 121 LSSNEISLELIERRIEEDMNGCSVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180
Query: 181 RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V+STN
Sbjct: 181 QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240
Query: 241 VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
+++H E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241 MEAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
Query: 301 WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
WMVQREK FS N GVGEN QLISPLC+ LECLDT RMFYNPFSG+LT +AE+TLPHVYG
Sbjct: 301 WMVQREKGFSRNFGVGENGQLISPLCMPLECLDTRLRMFYNPFSGDLTWNAEQTLPHVYG 360
Query: 361 GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
GILADEMGLGKTIELLACILSH MSVF+G KGF +E QQ+V QRT+FKRLKRERVECVC
Sbjct: 361 GILADEMGLGKTIELLACILSHPMSVFKGGKGFEDEMQQLVEDQRTKFKRLKRERVECVC 420
Query: 421 GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K K NK+N VNV
Sbjct: 421 GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKTNKRNTVNV 480
Query: 481 VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481 TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540
Query: 541 VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
VRDTSLS+ L VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541 VRDTSLSNPLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600
Query: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601 LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
Query: 661 SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
SPF+V+RWWVEVIRDPYERRDPG MEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661 SPFNVHRWWVEVIRDPYERRDPGTMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720
Query: 721 TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV VSSDAPSDILITH DAGKLL
Sbjct: 721 TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDAPSDILITHADAGKLL 780
Query: 781 STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781 NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840
Query: 841 GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841 GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900
Query: 901 DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
DQ Y RTCEVKAS+MDDSE DNH +KKQKV+ T Y PCSDTEKI+D+PL
Sbjct: 901 DQFYPRTCEVKASRMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960
Query: 961 -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
+PHRSS+YFND+ RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC L
Sbjct: 961 KEENYEPHRSSNYFNDNYLRKACEDMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020
Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080
Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELD+
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDQ 1140
Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200
Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
LAIAQAQVLNAHDEIKMATTRLCLR ND DDSAYAISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDADDSAYAISEHELPAASVQYSSDKFVSLAMLS 1320
Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380
Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
LSNQKMVFQCGH+ CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHITCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440
Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
LDPSTLH+TSRE E I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ATLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500
Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+ A+VPEL+SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPELRSAQVLLLLI 1560
Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHS 1620
Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1676
LNRSRE+SSFITGN KNQDQP TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680
BLAST of MS003347 vs. ExPASy TrEMBL
Match:
A0A1S3C3N1 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)
HSP 1 Score: 2823.5 bits (7318), Expect = 0.0e+00
Identity = 1437/1696 (84.73%), Postives = 1544/1696 (91.04%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLET-DKSSDIKLNEINEPFYVEVDPTGW 60
MGRRKQ +PH SGGIR E +P+TQ+DIN V ET ++SDIKL+E+NEP +VEVD +GW
Sbjct: 1 MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60
Query: 61 KSNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWP 120
SN+H DISEV L+D+R E+ FV F D S E+SRYSLRFRLCNVN LL RIKFGHWP
Sbjct: 61 YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120
Query: 121 ILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATL 180
+LSS+DI LELI++ +EE ACSVVLSGNFDGPD+AISGLVHLANLK MTLRPV G +
Sbjct: 121 VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180
Query: 181 SRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKST 240
S++TGSLRLRVE+LSSA DACESIFDNGRQLWKKSMM+TIAWLRPEV SE KYG+VKST
Sbjct: 181 SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240
Query: 241 NVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
N+D+H HE GD +SN+RKH+NFDT+ FYDAIKPSKD+P LDEDIPNLLPKLRPYQRRAA
Sbjct: 241 NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300
Query: 301 YWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVY 360
YWMVQREK FSGNSG+GENVQLISPLC+AL+CL+TC R+FYNPFSGNLTL+AE+ LPHVY
Sbjct: 301 YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360
Query: 361 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 420
GGILADEMGLGKTIELLACILSH+MSVFEG KGF E +Q+V QRTEFKRLKRERVEC+
Sbjct: 361 GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420
Query: 421 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 480
CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGR++KP+DTE GN ++KRN KN +N
Sbjct: 421 CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480
Query: 481 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYE 540
V+VR +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHTRPGSIKLLVYE
Sbjct: 481 VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540
Query: 541 GVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
GVRDTSL TLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541 GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
Query: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660
Query: 661 ASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
ASPF+V++WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661 ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
Query: 721 LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKL 780
L FSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKV DCVSSD PSD+L+TH DAGKL
Sbjct: 721 LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780
Query: 781 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840
Query: 841 AGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPL 900
AGIAII KKF EA SLYKEALELAE N EDFRLDPLLSIHIHHNLAEILP AV+QSQS L
Sbjct: 841 AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900
Query: 901 KDQSYSRTCEVKASKMDDSENYDNHT--MKKQKVSETSYAPCSD--TEKILDNPL----- 960
KDQ RTCEVKAS+MDDSE Y H MKKQKVSET YA CS+ T K++D+PL
Sbjct: 901 KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960
Query: 961 ----------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFY 1020
+PHRS+SYF++ RK CE MRQKYLAVFSSKLSIAQQEFTKS+MQV
Sbjct: 961 DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020
Query: 1021 VLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSI 1080
+ ENLN VWWLEAV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI
Sbjct: 1021 L----KDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSI 1080
Query: 1081 NALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVL 1140
+ALKYHVQ+GLDLLEASR VVLDRLLEIDQTMENPKEEDIERVRYC+NCQADSNGPPCVL
Sbjct: 1081 SALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVL 1140
Query: 1141 CELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRV 1200
CELDELFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQ+KN SSS+V
Sbjct: 1141 CELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKV 1200
Query: 1201 GYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEY 1260
G+EE NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLEYMRKEY
Sbjct: 1201 GHEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEY 1260
Query: 1261 GHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLS 1320
GHARSLAIAQA VLNAHDEIKMATTRLCLRDNDDD SA++ISEHELPAASVQYSSDKF+S
Sbjct: 1261 GHARSLAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMS 1320
Query: 1321 LAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESC 1380
LAMLSRVKGKLRYLKGLVQSKQ PL SSS+LALTQEPAI ST+MEQK+ENT KADEESC
Sbjct: 1321 LAMLSRVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESC 1380
Query: 1381 PVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFA 1440
P+CQEKLSNQKMVFQCGH+ CCKCLFAMTEKTL SKIQT+WVMCPTCRQHTDFGNIA+A
Sbjct: 1381 PICQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYA 1440
Query: 1441 DDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWN 1500
DDSKNE LDPSTL ETSR+HE ITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWN
Sbjct: 1441 DDSKNETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWN 1500
Query: 1501 DVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQV 1560
DVLDVL YAFAAN I+FIRMKGGRKSQTAISEFRGQKINAKEN KKR +PEL+SAQV
Sbjct: 1501 DVLDVLHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQV 1560
Query: 1561 LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE 1620
LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE
Sbjct: 1561 LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE 1620
Query: 1621 ESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAP 1675
ESI+KLNRSRE+SSFITGNAKNQDQPFLTLKEVESLFASKASPLG++DDK SETLR LAP
Sbjct: 1621 ESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAP 1680
BLAST of MS003347 vs. ExPASy TrEMBL
Match:
A0A0A0K8S1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 PE=3 SV=1)
HSP 1 Score: 2812.3 bits (7289), Expect = 0.0e+00
Identity = 1433/1697 (84.44%), Postives = 1534/1697 (90.39%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETD-KSSDIKLNEINEPFYVEVDPTGW 60
MGRRKQ +P+RSGGIR E + +TQ+DIN V+ET +SSDIKL+ +NEP +VEVD +GW
Sbjct: 101 MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 160
Query: 61 KSNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWP 120
SN+H DISEV L D+R EH FVGF D S E+SRYSLRFRLCNVN LL RIKFGHWP
Sbjct: 161 YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 220
Query: 121 ILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATL 180
+LSS+D LE I++ MEE ACSVVLSGN DGPD+AISGLVHLANLK MTLRPV G
Sbjct: 221 VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 280
Query: 181 SRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKST 240
S++ GSLRLRVE+LSSA DACESIFDNGRQLWKKSMM+TI WLRPEV SE KYG+VKS+
Sbjct: 281 SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 340
Query: 241 NVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
N+D+H HE GD +SN+RKH NFDT+GFYDAIKPSKD+PMLDEDIPNLLPKLRPYQRRAA
Sbjct: 341 NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 400
Query: 301 YWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVY 360
YWM+QREK FSGNSG+GENVQLISPLC+AL+CLDTC R+FYNPFSGNLTL+AE+ LPHVY
Sbjct: 401 YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 460
Query: 361 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 420
GGILADEMGLGKTIELLACILSH+MSVFEG KGF E QQ+V QRTEFKRLKRER+EC+
Sbjct: 461 GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 520
Query: 421 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 480
CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGR++K +DTE GN ++KRN +N +N
Sbjct: 521 CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 580
Query: 481 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYE 540
V+VR +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHT PGS+KLLVYE
Sbjct: 581 VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 640
Query: 541 GVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
GVRDTSL TLS QINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 641 GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 700
Query: 601 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 701 PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 760
Query: 661 ASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
ASPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 761 ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 820
Query: 721 LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKL 780
L FSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKV DCV+SD PSD+LITH DAGKL
Sbjct: 821 LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 880
Query: 781 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 881 LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 940
Query: 841 AGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPL 900
AGIAIIEKKFSEA SLYKEALE AE NNEDFRLDPLLSIHIHHNLAEILP AV+QSQSPL
Sbjct: 941 AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 1000
Query: 901 KDQSYSRTCEVKASKMDDSENYDNHT--MKKQKVSETSYAPCSD--TEKILDNPL----- 960
KDQ RTCEVKAS+MDDSE YD+H MKKQKVSET YA CS+ T K++D+PL
Sbjct: 1001 KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 1060
Query: 961 ----------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFY 1020
+PHRSS YF++ S RK CE MRQKYLAVFSSKLSIAQQEFTKS+MQV
Sbjct: 1061 DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1120
Query: 1021 VLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSI 1080
+ EN N VWWLEAV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI
Sbjct: 1121 L----KDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSI 1180
Query: 1081 NALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVL 1140
+ALKYHVQ+GLDLLEASR VVLDRLLEIDQTM++PKEEDIERVRYC+ CQADSNGPPCVL
Sbjct: 1181 SALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVL 1240
Query: 1141 CELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRV 1200
CELDELFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKN SSS+V
Sbjct: 1241 CELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKV 1300
Query: 1201 GYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEY 1260
G+EE NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLE+MRKEY
Sbjct: 1301 GHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEY 1360
Query: 1261 GHARSLAIAQAQVLNAHDEIKMATTRLCLR-DNDDDDSAYAISEHELPAASVQYSSDKFL 1320
GHARSLAIAQAQVLNAHDEIKMATTRL LR D+DDD SA+ ISEHELPAASVQYSSDKF+
Sbjct: 1361 GHARSLAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFM 1420
Query: 1321 SLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEES 1380
SLAMLSRVKGKLRYLKGLVQSKQ PL SSS+LALTQEPAI ST+ EQK+ENT KADEES
Sbjct: 1421 SLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEES 1480
Query: 1381 CPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAF 1440
CPVCQEKL+NQKMVFQCGH+ CCKCLFAMTEKTL SKIQT+WVMCPTCRQHTDFGNIA+
Sbjct: 1481 CPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAY 1540
Query: 1441 ADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSW 1500
ADDSKNE LDPST ETSREHE ITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSW
Sbjct: 1541 ADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSW 1600
Query: 1501 NDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQ 1560
NDVLDVL YAF AN ITFIRMKGGRKSQTAISEFRGQKINAKEN KKR +PEL+SAQ
Sbjct: 1601 NDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQ 1660
Query: 1561 VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV 1620
VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV
Sbjct: 1661 VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV 1720
Query: 1621 EESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLA 1675
EESI+KLNRSRE+SSFITGNAKNQDQPFLTLKEVESLFASKASPLG++DDK SETLR LA
Sbjct: 1721 EESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLA 1780
BLAST of MS003347 vs. TAIR 10
Match:
AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )
HSP 1 Score: 1761.9 bits (4562), Expect = 0.0e+00
Identity = 969/1708 (56.73%), Postives = 1205/1708 (70.55%), Query Frame = 0
Query: 1 MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
MGRRKQS+P R+ G L E+ + E + S D + +I++P+YV + +
Sbjct: 1 MGRRKQSKPQRAVG--LITPTGSESDRNQLPGDEAEGSGDKNVEDIDKPYYVNICLSSRV 60
Query: 61 S--NKHLDISEVVLMDLRFEHHFVG----FQFDDSFYESSRYSLRFRLCNVNEFLLGRIK 120
S ++ D++EVVL + VG F SLRFRLCNV F + RIK
Sbjct: 61 SEQQQNFDLAEVVLTNFSVRER-VGSSSTFTKPIEVDHDLDCSLRFRLCNVTNF-VDRIK 120
Query: 121 FGHWPILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPV 180
GHWP+LSSSDI+LEL+D + + + SV+ S +FDGP + +SGL HLA++KF+TLR +
Sbjct: 121 LGHWPVLSSSDITLELVDNKVSD-DEVGSVIWSASFDGPGEGVSGLAHLASIKFLTLRLM 180
Query: 181 MGATLSRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYG 240
G + S R+RVEML A DAC+S+ +N RQ+WKKSM+ ++WLRPEV +SEA+YG
Sbjct: 181 PG---NEGLLSPRVRVEMLQQAFDACDSLLENTRQIWKKSMIHVMSWLRPEVMTSEARYG 240
Query: 241 LVKSTNVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPY 300
+ D S D + ++ K +FD FY+AIKPSK D ML +DI +LLP+LRPY
Sbjct: 241 -TRFNVKDIESSVVTEDETLDSSKQSSFDAAAFYEAIKPSKTDAMLGDDIADLLPELRPY 300
Query: 301 QRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKT 360
QRRAAYWMVQRE+ G E+ Q ISPL I++ LD+ T+MF NPFSGN++L+ E
Sbjct: 301 QRRAAYWMVQRERGDPITLGDKEDNQFISPLSISVGFLDSATKMFLNPFSGNISLTPEYF 360
Query: 361 LPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRE 420
P + GGILADEMGLGKT+ELLACI SHR E E N S + +RLKRE
Sbjct: 361 SPRIQGGILADEMGLGKTVELLACIFSHRKPA-EDEISVSNGS-SVTDVLNAGLRRLKRE 420
Query: 421 RVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKP---IDTEGGNCIKKK 480
RVEC+CGAVSE+H+YKG+WVQCD+CDAWQHADCVGYSPKG+ K ID + +K
Sbjct: 421 RVECICGAVSESHKYKGVWVQCDLCDAWQHADCVGYSPKGKGKKDSQHIDEKA----SQK 480
Query: 481 RNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPG 540
++KK+ ++VR+G+++C C EL+Q T +P++T ATLIVCPAPIL QWH+EI RHTR G
Sbjct: 481 KSKKDATEIIVREGEYICQMCSELLQVTASPISTGATLIVCPAPILPQWHSEITRHTRLG 540
Query: 541 SIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQ 600
S+ +YEGVR+ SLS + I +L+N+DI+LT+YDVLKEDL+HD DRH+GDR +RFQ
Sbjct: 541 SLITCIYEGVRNASLSEEPMIDITELLNADIVLTTYDVLKEDLTHDFDRHDGDRHCLRFQ 600
Query: 601 KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 660
KRYPVIPTPLTRIFWWRICLDE QMVESNA AATEMA RLY HRWCITGTPIQRKL+DL
Sbjct: 601 KRYPVIPTPLTRIFWWRICLDEAQMVESNAAAATEMALRLYTKHRWCITGTPIQRKLDDL 660
Query: 661 YGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLP 720
+GLL+F+KA+PFDV RWW+EVIRDPYERRD AMEFTHKFFKQ+MWRS K+HV DELQLP
Sbjct: 661 FGLLKFLKANPFDVSRWWIEVIRDPYERRDTKAMEFTHKFFKQVMWRSSKVHVADELQLP 720
Query: 721 PQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILI 780
PQEE V+WL FS IEEHFY RQH+TCVSYAREVI+ LK D +KR SSD P L+
Sbjct: 721 PQEECVSWLKFSAIEEHFYSRQHDTCVSYAREVIETLKRDILKR---GHTSSDNP---LV 780
Query: 781 THTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR 840
TH +A KLL++LLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLV KT+ EGEEALR
Sbjct: 781 THAEAAKLLNSLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVKKTQSEGEEALRV 840
Query: 841 SVVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSA 900
+VALN +A IA+++++FSEAVSLYKEAL + E + EDFRLDPLL+IHI HNLAEILP A
Sbjct: 841 LIVALNGIAAIAMLKQEFSEAVSLYKEALSITEEHAEDFRLDPLLNIHILHNLAEILPMA 900
Query: 901 VDQSQSPLKDQSYSRTCEVKASKMDDSENYDNH-TMKKQKVSETSYAPCSDTEKILDNPL 960
+S S S E K DD D+H K+Q+++E +E +
Sbjct: 901 ----KSYGGKLSASGRPETKIDVKDD----DHHRASKRQRINELESLTHDSSETV----- 960
Query: 961 KPHRSSSYFNDSSFRK-------------ACETMRQKYLAVFSSKLSIAQQEFTKSHMQV 1020
H+ + D+ +K C+T++ KYL+ F+SKLS AQ EF KS+ QV
Sbjct: 961 --HQREAIAPDNGLKKDGECHEECKTLDIVCDTLKVKYLSAFNSKLSAAQHEFKKSYNQV 1020
Query: 1021 CFYVLLLYSRLENLNK---VWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVG 1080
L N+ K VWWL+A+ EQNKDF EL RKIEEA+ GN+NNS SSR
Sbjct: 1021 S-------ESLSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSSRES 1080
Query: 1081 SRFRSINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSN 1140
SRFR+I+ +K H+QT +D+LE SR V+DR+LEIDQTME PK EDIER+ C+ C +S+
Sbjct: 1081 SRFRTIHGMKLHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNKNSD 1140
Query: 1141 GPPCVLCELDELFQEYEARLFRLNKVQGGM--VSSVEEAVEAQKKKSALNRFYWSLLQQN 1200
GPPC+ CELDELFQEYEARLFRLNK + G+ +++ EE V QKK+ A N F + L ++
Sbjct: 1141 GPPCIHCELDELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLSSRS 1200
Query: 1201 KNLSSSRVGYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHL 1260
K+L++SR EE KR+ G+ V++SK PSE E+VLGVI+N CKT L +ES AA K LH
Sbjct: 1201 KDLNASRGDDEEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKHLHT 1260
Query: 1261 LEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQ 1320
LE MRKEY HAR LA QAQ+L A+DEI M+T RL LR+++DD S YA+ EL ASV
Sbjct: 1261 LEVMRKEYVHARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVASVL 1320
Query: 1321 YSSDKFLSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENT 1380
++DKF++ + L +KGKLRYLKGL++SKQ S + E S EQ+SEN
Sbjct: 1321 NTNDKFMAQSSLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQESENL 1380
Query: 1381 PKADEESCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHT 1440
K D E+CP+C E L NQKMVFQCGH CC C FAMTE+ +Q +WVMCP CRQHT
Sbjct: 1381 LKRD-EACPICHEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQ-KWVMCPICRQHT 1440
Query: 1441 DFGNIAFADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAK 1500
D NIA+ADD +N S+ + ++ E + VQGSYGTKIEAV RRILWIK +D + K
Sbjct: 1441 DVRNIAYADDRRNS----SSSDQDHKDSEASLVVQGSYGTKIEAVTRRILWIKSSDPQTK 1500
Query: 1501 VLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPE 1560
VLVFSSWNDVLDVL++AFAAN IT IRMKGGRKSQTAIS+F+G + KE K + E
Sbjct: 1501 VLVFSSWNDVLDVLEHAFAANSITCIRMKGGRKSQTAISKFKGSE---KETQKTNSHQKE 1560
Query: 1561 LKSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFI 1620
KS QVLLLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVHRIGQE T VHRF+
Sbjct: 1561 EKSIQVLLLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTLVHRFL 1620
Query: 1621 VKDTVEESIHKLNRSREN--SSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDD--- 1676
V TVEESI+KLNR++ SSF + N KNQDQ FLTLK++ESLFAS + + +
Sbjct: 1621 VSGTVEESIYKLNRNKNTNLSSFSSRNTKNQDQQFLTLKDLESLFASPTAETAEMEQNPG 1656
BLAST of MS003347 vs. TAIR 10
Match:
AT5G22750.1 (DNA/RNA helicase protein )
HSP 1 Score: 172.6 bits (436), Expect = 2.8e-42
Identity = 142/525 (27.05%), Postives = 213/525 (40.57%), Query Frame = 0
Query: 287 LLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRM--FYNPFS 346
LL +LRPYQ++A +WM Q EK GN E ++ P C CL + + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEK---GNC-TDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403
Query: 347 GNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSV---------FEGEKGFGN 406
G+ T+ TL GGILAD MGLGKT+ ++ +L+H +EG+K +
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISS 463
Query: 407 ESQQIVGG--QRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPK 466
+ + T+F +G+ +
Sbjct: 464 SVDDLTSPPVKATKF---------------------------------------LGFDKR 523
Query: 467 GRVLKPIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCP 526
K + GGN LIVCP
Sbjct: 524 LLEQKSVLQNGGN------------------------------------------LIVCP 583
Query: 527 APILFQWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKED 586
+L QW EI H +PGS+ + V+ G + LS SD+++T+Y VL +
Sbjct: 584 MTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSE 643
Query: 587 LSHD-SDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLY 646
S + S HEG + + W+RI LDE ++++ + + A+ L
Sbjct: 644 FSQENSADHEG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALV 703
Query: 647 ASHRWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFF 706
A RWC+TGTPIQ LEDLY LLRF++ P+ + WW ++++ P+E D ++
Sbjct: 704 ADRRWCLTGTPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSIL 736
Query: 707 KQIMWRSLKIHVTDE----LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGL 766
K IM R K E L LPP + +V + S E FY + R ++
Sbjct: 764 KPIMLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK-- 736
Query: 767 KDDFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
D FV++ + H A +L LL+LRQ C HP
Sbjct: 824 FDQFVEQG-------------KVLHNYA-SILELLLRLRQCCDHP 736
HSP 2 Score: 107.5 bits (267), Expect = 1.1e-22
Identity = 82/311 (26.37%), Postives = 145/311 (46.62%), Query Frame = 0
Query: 1327 GSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLF 1386
G SS L + +++ E K ++ CP+C E L + ++ C H +C +CL
Sbjct: 761 GKSSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLL 820
Query: 1387 AMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREHETFITV 1446
A + +CP CR SK E++ T E + V
Sbjct: 821 ASWRNSTSG--------LCPVCRNTV----------SKQELITAPT------ESRFQVDV 880
Query: 1447 QGSY--GTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGR 1506
+ ++ +KI A++ + ++ + SK+ ++FS W LD+LQ + N +F+R+ G
Sbjct: 881 EKNWVESSKITALLEELEGLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG-- 940
Query: 1507 KSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1566
++ ++ K + E S VLL+ ++ G G+NL A + +++P
Sbjct: 941 ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1000
Query: 1567 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKNQDQP 1626
NPA E QA+ R+HRIGQ + + RFIVK TVEE + + ++ I+G +Q+
Sbjct: 1001 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQR--MISGALTDQEVR 1028
Query: 1627 FLTLKEVESLF 1636
++E++ LF
Sbjct: 1061 SARIEELKMLF 1028
BLAST of MS003347 vs. TAIR 10
Match:
AT5G43530.1 (Helicase protein with RING/U-box domain )
HSP 1 Score: 163.3 bits (412), Expect = 1.7e-39
Identity = 144/523 (27.53%), Postives = 208/523 (39.77%), Query Frame = 0
Query: 291 LRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALE-CLDTCTRMFYNPFSGNLTL 350
LRPYQ++A YWM + EK V + + + P A C + ++ N FSG T+
Sbjct: 616 LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675
Query: 351 SAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFK 410
GGILAD MGLGKT+ +A IL+ G GN +
Sbjct: 676 QFPTATQMARGGILADAMGLGKTVMTIALILA--------RPGRGNPENE---------- 735
Query: 411 RLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIK 470
D+ A +AD
Sbjct: 736 ---------------------------DVLVADVNAD----------------------- 795
Query: 471 KKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTR 530
KRN+K E+ A T A TLI+CP +L QW E+ H++
Sbjct: 796 -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855
Query: 531 PGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMR 590
P ++ +LVY G T + ++ + D++LT+Y VL D
Sbjct: 856 PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915
Query: 591 FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
+ + RI W+RI LDE ++S T A + L + RWC+TGTP+Q KLE
Sbjct: 916 ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975
Query: 651 DLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 710
DLY LL F+ P+ + WW ++I+ PYE DP ++ + +M R K E
Sbjct: 976 DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000
Query: 711 --LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDA 770
L+LPP + QV S E FY + R +Q D FV A
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFV-----------A 1000
Query: 771 PSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 809
+L + + +L LL+LRQ C HP + S S Q + +
Sbjct: 1096 QGKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000
HSP 2 Score: 106.3 bits (264), Expect = 2.4e-22
Identity = 87/322 (27.02%), Postives = 141/322 (43.79%), Query Frame = 0
Query: 1320 SKQNTPLGSSSNLALTQEPAIMSTS------MEQKSENTPKADEESCPVCQEKLSNQKMV 1379
S+Q L S + L P +S + +E+ ++ + + CP+C E ++ ++
Sbjct: 994 SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053
Query: 1380 FQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTL 1439
C H +C +CL S +CP CR + E++
Sbjct: 1054 TPCAHRMCRECLLT--------SWRSPSCGLCPICRTIL----------KRTELIS---- 1113
Query: 1440 HETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 1499
T + +K+ +++ + IK + S K +VFS W LD+L+
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173
Query: 1500 GITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLL 1559
G F+R G K+ K K + E K +LL+ ++ G GLNL
Sbjct: 1174 GFEFLRFDG--------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLT 1233
Query: 1560 EAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSF 1619
A V L++P NPA E QAI R+HRIGQ+ FV RFIVKDTVEE + ++ ++
Sbjct: 1234 AASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQR--M 1276
Query: 1620 ITGNAKNQDQPFLTLKEVESLF 1636
I G +++ L+E++ LF
Sbjct: 1294 IAGALTDEEVRSARLEELKMLF 1276
BLAST of MS003347 vs. TAIR 10
Match:
AT1G61140.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )
HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 114/463 (24.62%), Postives = 182/463 (39.31%), Query Frame = 0
Query: 360 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 419
GGILAD+ GLGKT+ +A IL R +Q + E L+ E EC
Sbjct: 579 GGILADDQGLGKTVSTIALILKER----------SKPAQACEESTKKEIFDLESETGECA 638
Query: 420 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 479
S ++ ++H+ ++L + GG+ + K
Sbjct: 639 PLKPSGRSKH------------FEHS---------QLLSNENKVGGDSVGK--------- 698
Query: 480 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRH-TRPGSIKLLVY 539
T P A TL+VCP ++ QW E+ + T ++ +LVY
Sbjct: 699 ------------------VTGRPAA--GTLVVCPTSVMRQWADELHKKVTSEANLSVLVY 758
Query: 540 EGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDR-----HEGDRRFMRF--- 599
G T L+ D+V + + S +V K+ L D D H+G F
Sbjct: 759 HGSSRTKDPHELAKY--DVVVTTFSIVSMEVPKQPLVDDEDEEKDGVHDGGTAATGFCSN 818
Query: 600 -QKRYP--------------VIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASH 659
+++YP + PL ++ W+R+ LDE Q +++ T L A
Sbjct: 819 KKRKYPPDSKKKGSKKKKVEFLSGPLAKVSWFRVVLDEAQSIKNYKTQVARACWGLRAKR 878
Query: 660 RWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQI 719
RWC++GTPIQ ++DLY RF+K P+ Y + I++P R + K +
Sbjct: 879 RWCLSGTPIQNSIDDLYSYFRFLKYDPYSSYVLFCSTIKNPITRNPVKGYQKLQAILKTV 938
Query: 720 MWRSLKIHVTDE---LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQG--LKD 779
M R K + D + LPP+ ++ + F+ E FY + + RE + +K
Sbjct: 939 MLRRTKGSLLDGKPIISLPPKSIELRKVDFTVEERDFYSKLEAESRTQFREYAEAGTVKQ 955
Query: 780 DFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
++V +L LL+LRQAC HP
Sbjct: 999 NYV------------------------NILLMLLRLRQACDHP 955
HSP 2 Score: 72.8 bits (177), Expect = 3.0e-12
Identity = 49/166 (29.52%), Postives = 82/166 (49.40%), Query Frame = 0
Query: 1472 KVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVP 1531
K +VFS W +L++L+ + ++ I + R+ G ++ K
Sbjct: 1125 KAIVFSQWTKMLNLLEASLVSSHIQYRRLDG--------------TMSVAARDKAVQDFN 1184
Query: 1532 ELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF 1591
L V+++ ++ + GLN++ A HV++++ NP E QAI R HRIGQ V RF
Sbjct: 1185 TLPEVTVMIMSLKAASLGLNMVAACHVLMLDLWWNPTTEDQAIDRAHRIGQTRPVTVVRF 1244
Query: 1592 IVKDTVEESIHKLNRSREN--SSFITGNAKNQDQPFLTLKEVESLF 1636
VKDTVE+ I L + + +S + K Q LT++++ LF
Sbjct: 1245 TVKDTVEDRILALQQKKRMMVASAFGEDEKGSRQSHLTVEDLSYLF 1276
BLAST of MS003347 vs. TAIR 10
Match:
AT1G61140.2 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )
HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 114/463 (24.62%), Postives = 182/463 (39.31%), Query Frame = 0
Query: 360 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 419
GGILAD+ GLGKT+ +A IL R +Q + E L+ E EC
Sbjct: 586 GGILADDQGLGKTVSTIALILKER----------SKPAQACEESTKKEIFDLESETGECA 645
Query: 420 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 479
S ++ ++H+ ++L + GG+ + K
Sbjct: 646 PLKPSGRSKH------------FEHS---------QLLSNENKVGGDSVGK--------- 705
Query: 480 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRH-TRPGSIKLLVY 539
T P A TL+VCP ++ QW E+ + T ++ +LVY
Sbjct: 706 ------------------VTGRPAA--GTLVVCPTSVMRQWADELHKKVTSEANLSVLVY 765
Query: 540 EGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDR-----HEGDRRFMRF--- 599
G T L+ D+V + + S +V K+ L D D H+G F
Sbjct: 766 HGSSRTKDPHELAKY--DVVVTTFSIVSMEVPKQPLVDDEDEEKDGVHDGGTAATGFCSN 825
Query: 600 -QKRYP--------------VIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASH 659
+++YP + PL ++ W+R+ LDE Q +++ T L A
Sbjct: 826 KKRKYPPDSKKKGSKKKKVEFLSGPLAKVSWFRVVLDEAQSIKNYKTQVARACWGLRAKR 885
Query: 660 RWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQI 719
RWC++GTPIQ ++DLY RF+K P+ Y + I++P R + K +
Sbjct: 886 RWCLSGTPIQNSIDDLYSYFRFLKYDPYSSYVLFCSTIKNPITRNPVKGYQKLQAILKTV 945
Query: 720 MWRSLKIHVTDE---LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQG--LKD 779
M R K + D + LPP+ ++ + F+ E FY + + RE + +K
Sbjct: 946 MLRRTKGSLLDGKPIISLPPKSIELRKVDFTVEERDFYSKLEAESRTQFREYAEAGTVKQ 962
Query: 780 DFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
++V +L LL+LRQAC HP
Sbjct: 1006 NYV------------------------NILLMLLRLRQACDHP 962
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022134046.1 | 0.0e+00 | 99.04 | E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiqu... | [more] |
XP_038884903.1 | 0.0e+00 | 86.10 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1... | [more] |
XP_023551402.1 | 0.0e+00 | 85.45 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_022938761.1 | 0.0e+00 | 85.10 | E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata] | [more] |
KAG6578487.1 | 0.0e+00 | 84.92 | E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
Match Name | E-value | Identity | Description | |
Q149N8 | 1.2e-146 | 30.65 | E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2 | [more] |
Q7TPQ3 | 3.2e-144 | 30.19 | E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1 | [more] |
Q9UTL9 | 6.4e-60 | 23.10 | Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (str... | [more] |
Q9FNI6 | 3.9e-41 | 27.05 | DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1 | [more] |
Q9FIY7 | 2.4e-38 | 27.53 | DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BYG6 | 0.0e+00 | 99.04 | E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415... | [more] |
A0A6J1FE16 | 0.0e+00 | 85.10 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1JXA7 | 0.0e+00 | 84.63 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A1S3C3N1 | 0.0e+00 | 84.73 | E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... | [more] |
A0A0A0K8S1 | 0.0e+00 | 84.44 | RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 P... | [more] |
Match Name | E-value | Identity | Description | |
AT2G40770.1 | 0.0e+00 | 56.73 | zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding | [more] |
AT5G22750.1 | 2.8e-42 | 27.05 | DNA/RNA helicase protein | [more] |
AT5G43530.1 | 1.7e-39 | 27.53 | Helicase protein with RING/U-box domain | [more] |
AT1G61140.1 | 1.9e-27 | 24.62 | SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... | [more] |
AT1G61140.2 | 1.9e-27 | 24.62 | SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... | [more] |