MS003347 (gene) Bitter gourd (TR) v1

Overview
NameMS003347
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase SHPRH
Locationscaffold234: 2265391 .. 2275496 (+)
RNA-Seq ExpressionMS003347
SyntenyMS003347
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGAAGGAAGCAAAGTCGACCTCATCGCTCTGGTGGAATAAGACTTGAAATTCATGCGAATCCCGAGACACAGGTTGATATAAATGTGGTTTTGGAAACTGATAAATCATCAGACATCAAACTTAATGAGATTAATGAGCCCTTTTACGTTGAAGTTGATCCGACTGGGTGGAAGTCCAATAAGCACCTTGATATTTCTGAAGTTGTTCTAATGGATTTGAGGTTTGAACACCATTTTGTCGGTTTTCAATTTGATGATAGTTTTTATGAGAGCTCGAGGTACTCATTAAGGTTTCGGCTGTGCAATGTAAATGAGTTTCTCCTTGGTCGTATTAAGTTTGGTCATTGGCCAATTTTATCTTCAAGTGATATATCTTTGGAACTCATCGATAAAGGCATGGAAGAAGGTACGGATGCTTGCTCAGTGGTTTTATCAGGGAACTTTGATGGACCAGATGATGCCATTTCTGGCCTTGTTCATCTTGCAAATTTGAAGTTCATGACATTAAGGCCAGTTATGGGTGCTACCCTTTCCCGTAGTACAGGATCTCTCAGGTTGAGGGTGGAAATGTTATCGAGTGCCCTTGATGCTTGTGAGTCAATATTTGATAACGGCAGGCAGTTGTGGAAAAAGAGTATGATGAGTACCATTGCTTGGTTGCGTCCTGAAGTAGAATCATCTGAGGCCAAGTATGGACTTGTTAAATCAACTAATGTGGACAGTCATTCGCTTCATGAGATGGGGGATGGCTCGTCCAACACTAGGAAACACATAAATTTCGATACAGTTGGCTTTTATGATGCCATTAAGCCATCAAAGTGAGTGTATTTGATATTCACGTACTTGTGTATTCTGTTTTTATTAACTTACAGAATGTTTGACATAAGTTCAATGTAGAGATGATCCTATGCTTGATGAAGACATACCCAATTTGCTTCCAAAGCTAAGACCATATCAACGTCGTGCTGCCTATTGGATGGTACAACGGGAGAAAAGATTTTCTGGAAACTCAGGTGTGGGGGAAAACGTGCAGCTTATTTCTCCCTTGTGTATAGCCCTGGAATGTCTTGATACTTGTACGAGAATGTTTTATAATCCTTTCAGGTATTTACTTTCAAGCATGGCCGAGTGGAGTGAATAAAGTTGAACAATATGTGTTTGTTGATGTAGTTACGAAGACTTACTTTTCCTCTTGTATGCTTACTTAAGTTTATCTCCAGTGGAAATCTCACTTTGAGTGCAGAGAAAACTTTGCCGCACGTTTACGGGGGAATTCTAGCTGGTAATTGCCACCAACTAGAAATTATTACATTTTCCTAAGCTAGGTATTTCATTTAATATCAGCACTCAGTTCTGATACAATTATCCATTCTTATGGTCTTTATTTTGTCTGAATAATATGATGTCTGCAGATGAGATGGGTCTAGGAAAAACTATTGAACTACTTGCATGCATACTTTCTCACCGGATGTCAGTGTTTGAAGGTGAAAAAGGTTTTGGTAATGAGTCACAACAAATTGTTGGAGGCCAGAGAACTGAATTTAAAAGATTGAAAAGGGAGCGTGTTGAGTGTGTATGTGGAGCTGTTAGTGAAAATCACAGGTACAAGGGGTTGTGGGTACAATGTGACATCTGTGATGCATGGCAACATGCTGACTGCGTTGGTTATTCACCTAAAGGAAGAGTTCTCAAACCCATTGATACAGAGGGTGGGAACTGTATAAAGAAAAAGAGAAACAAAAAAAATAGAGTAAATGTAGTCGTTAGAGATGGGCAACATGTTTGTTTGCCATGCTTAGAGCTGATGCAAGCTACTGACACCCCAATGGCTACAGTTGCAACTCTAATTGTCTGTCCAGCTCCCATATTATTCCAATGGCATGCTGAGATTTTACGGTATACTCTTATTTGACCATATTCTACTTATCTCATTTCATTGCATTTGATTTTTTGGACACCTACAATTTTACACCTCGATTAACTTGAAATGTTGTGACTAAATTATGTTCAATAAGTTGCTGTCTAATATACTTTTTTCTTCCAATAAGAAACACTAGATTACTTAATAAATAATGGGAAAAGGAATAAAAGGAGGCGAGGGGGATTTGCTCATTCTCCTATAGCCAGCAGCCAAGAATGATAAAAATACCTTTGCTGTCTAATATCCTTCAGTGTTAACTAAATGAGATTCTTTTCATCTGAGCCTGATGTTTCCTAATTCCTATGATCTCTGGTTGAGGACATCATACATAAATTCACTTACGCAATGCTAAGCAATTAGAAAGCAATATGATAGGTCTTGTTTACACAGAGCCAATTTTTTACGACAATTTGCTAATCAGTACTTCTGGTTAAATGTGTACGGAGACTTTTATGGACCCATGAGAAGGAATCAAAATGACCATATTGGAGTTGGTTTAGTGCTGAAATTGAAAGATTACTTATGAAAAAAGTTTAAAAATTATGGCTGCATAGCCGTTACTCTCTTTCAATTTCAACTTAGTAGATTATGACTGCACCTTTCTTGTTGTGGCATGCATGCTTATCTCTTTAATTGGACGTTTTTGTGAAAAGTTCATCTATTTAAAACTAGTTCACTTAAAAGTTTTCATTTGAAATGGTAGTGCAATCTGACTTTTCAATTGTTATTTATCATCCCAGTCATACTCGTCCAGGTTCAATAAAACTACTTGTCTACGAAGGAGTGAGGGATACTTCTCTTTCTAGCACATTGTCGGTGCAGATTAACGATCTCGTCAATTCTGATATAATTTTGACATCATATGATGTTCTTAAAGAGGACCTATCCCATGATTCTGATAGACACGAAGGTGATCGACGATTCATGAGATTTCAGAAGAGGTCTGTTCTTACCTTGCTGAGAAATGTTGCTTGGAATTTAATATAATTACTTCTTTCTTACTTCAAAACTAGTCAGAGTAAGAATTTAGTTTAAGCCTTTAAGGTTGTAGTGGTATTATATACTTTTAACCAGTTTGCTTCTAGGGACTTATTTTCTTGATATCCATCACATATCAATCTTTGTGCCCTCACTATCTGTGTTTACTGCATGCAAACAATGTGAAATGTAGTTGTACTGGCATGCTTGTGTTTCAACTTTCAAGTTATTTTAAGTTTTTAACCTGATGGTTTATGTTCCTAAATATCCTTTTTTCTTCTTTTAGCAACCTGTTTAGGTAAATCTTGAATATGACAACGATTCATCCTTCACTGTGTTATTTTATCTATTTCCATACAGGAGGTCTAGGGAGAAATATTTAAGGAATTATCAATTTGTTTTCTTCAATGTAATCCTCTTTTGAAATTGAAAACTCTTGACAATATATGTGGGCTGTGGCCACTTCATTTTAGGTACCCTGTTATCCCTACTCCTCTCACCAGGATCTTTTGGTGGAGGATCTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCTACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCATCGCTGGTGCATTACAGGAACTCCCATTCAAAGGAAACTTGAAGATTTATATGGATTACTAAGATTTGTGAAAGCAAGTCCATTTGATGTTTATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGGTACGTGGCATTCAAAGATTTAATGTTCAGTCGTAGTTAGGGAAAAGGGAAAAGGGAAAGAGAGAGACAAAATGCCATATAAACTTTGTTATTATAACAACTCTGCCATTCTTAGTCGTTTTTATAAATGTTCATTTTTTGTCCTTTTTCTTTATCTTTAGGTCTTATTCAGGGATTGCTTCCTAGCTATCCTTGACCCACGTTATTTTGCATTTTTGACATACACTGGTTTCTTTATTTTTTACCAACAACTTCTGCAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGCTCTTTAAAAATACATGTTACGGATGAATTGCAGCTACCTCCTCAGGAGGAGCAAGTCACTTGGCTAACGTTTTCACCAATTGAAGAGCACTTCTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAGGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCGCAGGTTGTTAGTGGTTGATTATGACTTCATTCAAGAGTCATTTCTTTGTTCATCACTATGATTTACATCACAAGGGAAAGTGTTATATCTTACATTGTCGTACTGCTATCTTTTTTCAGATTGTGTCTCTTCTGATGCACCATCCGATATTCTCATCACCCATACCGATGCTGGAAAGCTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCACAAGTAGGAAGTTCGGGACTGCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATACTGATGGTATTCCTCAGATCAAGATATGTGAGTTTTCATGAAATGAAATCTGGTGTAAGACTTTAGTCTAAGTGGTTTTACTTCTCAGGTTCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCGGTTGTTGCTTTAAATGCACTTGCTGGGATAGCTATAATTGAGAAGAAATTTTCTGAAGCCGTCTCACTATATAAAGAAGCTCTAGAATTGGCTGAGGGGAACAATGAAGATTTTCGCTTAGATCCTTTGTTGAGCATCCACATCCATCACAATCTTGCTGAGATACTACCATCAGCTGTGGACCAGTCGCAATCTCCTTTAAAGGATCAATCTTATTCAAGAACTTGTGAAGTGAAAGCCTCCAAGATGGATGATTCTGAAAATTATGATAATCATACCATGAAGAAACAAAAAGTTAGTGAGACCTCGTATGCTCCTTGTAGTGATACAGAGAAGATATTAGACAATCCTCTTAAGCCTCATCGGTCAAGTAGTTACTTCAATGATAGTTCTTTTAGAAAAGCATGTGAGACTATGAGGCAGAAATATCTAGCTGTCTTTTCCTCAAAGCTATCTATTGCCCAACAAGAGTTCACAAAATCCCACATGCAGGTATGTTTCTACGTGCTACTCCTTTACTCTAGGCTGTTACAATGTATATCGTCATTCATAATAAAGTTCATTGTAAGATCTTTTTTGTGGTAAAGATTGATGGTATGATCACCATTTGGGTTCTACTATTTGGTTGCTTATCGATCCTTTTTATCATTGGGTGCTTAAAATGTAGTTTATTTCTTTCCGATGGATAATCTTGGTGTATTAATTTCACTTGGAAACTACTTTAGTTTAGTACAGCCATGATGAGTATTACATATATCACTAATTTTTATGGCTAATTTGTTCAATACAAACTGAAGGTCTGCAGTGAACTCAGAGAAAGGGAAAATCTTAACAAAGTTTGGTGGCTGGAAGCAGTTGATCATGCTGAACAGAACAAGGATTTCTATCATGAGTTGATAAGAAAGATTGAAGAGGCCGTATCTGGAAACATTAACAACTCGAGATCATCTAGAGTGGGATCCCGGTAAACCATGTCAAACTCTCTGCATTATATCATTACTTTGTCCTTCTCCCCAGTCATGATTACTGATTGATTTCCTAGCTTGATAACAGTTTCCGCAGCATTAATGCTTTAAAGTATCATGTTCAGACTGGTTTGGATCTTTTGGAAGCTTCTAGGAATGTTGTGCTTGATCGACTTTTAGAAATTGATCAGACGATGGAAAATCCAAAAGAAGAAGATATTGAACGTGTTAGGTATTGTCAAAATTGCCAGGCTGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGACGAACTATTTCAGGTTCTTCTTCCTGATTTGTACAATTACCTTAAATCTTTCTCTTTGACTTGCATTACGAATATAGGCTCTCCTTCTTTTCCAGGAATATGAGGCAAGGCTCTTCAGGCTTAATAAAGTACAAGGAGGAATGGTTTCTTCTGTTGAAGAGGCAGTTGAAGCCCAGAAGAAGAAGTCTGCACTTAATCGTTTCTATTGGAGTCTGTTGCAGCAAAATAAAAATTTAAGTTCATCTAGAGTTGGATATGAAGAATCTAACAAAAGAGATGTTGGAGAAAAAGTTATGGTTAGTTTTTCTTCTTTACCTACTTTTTTCTTAGAAAGGGGGCCCCAGGTTGGTGACATCTGGCTTATAATATGATTATTTTTTATTTCTCGTGATGGAAATTGTGAACTGTTTGATTTCTTGTACTCTTTTTAAAAAAAAAAAAATGAAGTGGGTTCCCTCTCTTTCAAATTATTGCTTATTTTTGTAGTGTATATATTTTGTCGAACAACTAAAAAAAAAAAGAAAAAAAAAGCAAGAGAGAGAGAGAGAAAGAAATATGTATTCTTGTTTAACAACCCTCATATTATCTGGCAACAGACCCTGTTCCAAATTTGTACGTTGTTGATCTGATTTTTAGGTGTCTAAGCATCCTTCTGAATTGGAGGTCGTTCTTGGTGTTATCAAGAACTTCTGTAAAACACAACTAGGGAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACATCTTCTGGAGGTATTATTTGGTCAAATTCTATTTTATGAGAAAAAATACCCTTCAAATGTCCTGTGATGACTGATTCCTCGACACCATTTTTCGCATGAAGAGCTTTCTAATGTTCTTAGAGCAAATAATTCGTCTTATTAATTTTATATTTTGGAGCCCTAATTAATTTATAAGACTTGGGCATCGTGATAGAGAGGTGAACCATATAATTGATCTTCTATAATTTAGAAGACACCAAACAGATATTTTTTGCTTTCTTTTTTCCTGATATCTGGAGTGTTCGGGCTAGCTTGCACGTACCTTGACTATTCTTACGAGGTCCTACTACATTTGGTTGTCAAGCTAACTCATAGGATCAGGGTTTAGGGCTTAGAGTTCGGAACATATATTTAAATCTTATATGTTGATATAGGCATAAACTATTTTAAAATTTTAAGACAATTCTTGGTCCTAGAATTTATTATACATTGAGATGAAAACTTACTGGATTTTTAACGATTAGATTTCTTTTTCAATTTGTCTAGTACATGCGAAAGGAGTATGGACATGCAAGGTCCTTAGCCATTGCTCAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTATGCTTAAGGGACAATGACGATGACGACTCAGCTTATGCGATTAGTGAACATGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCTTGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGTTAAAATGTTTCCAGATCTGTTTAGTGCATTGGAAGTTTTCATATCGAGAATAGTTATGTTGTTACTCACAATTTCTATTATTTCCAGGGTTTGGTGCAATCTAAACAAAACACACCATTAGGTAGTTCTAGTAATCTAGCGTTAACTCAAGAGCCGGCTATCATGTCAACATCTATGGAACAAAAAAGTGAGAACACACCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAATTAAGCAATCAAAAGATGGTTTTTCAATGTGGACATATGATATGCTGTAAATGTGAGTGTTGGTTTTTACATCAGTGCTCGTCTTTTGAGTCACCTGCATATGTGTTTTATGATCTACATTAGTTCACAAGATAATTACATCTATGCAACTGCCAAATTCCTGGATGAAAATGTGATTTAGTTGATTCAGCACTTACATTTAGAGTGAGTTTGTTTTGAGAGTTGTATTTTTTAAGGGAAACTCTCATTTTCGTTTTGAGAGTTGTATTTTTAAGGGAAACTCTCATTTTATTAGCATTCTATATTGCTTTAAGTTTTTTTTTTTTTTAAGTTCAGAATCACTTCCAAACACCTTAAAAAAGTACTTTTAATTGCTTATTTCAAAAGTCATATCAAATTCAATGATTTTGTGCTTATAAATCCTTTAGAACGGAAGTGAGGGTGAAATCTTATTCTATATATTCATCTTTGAACCTAGTGCGCTTCCTGCTTCTGAAAACTATCAACTTGTGGTTTCATCCATTTCTGAATGGTTACTTCATCATGTATTCTAACGGCCTTTTTTCCTGACAGGTTTGTTCGCAATGACTGAAAAGACCCTGCAGGATAGTAAAATTCAAACTCAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGTAATATTGCTTTTGCGGATGATAGTAAAAATGAAATACTTGATCCTTCAACATTGCACGAAACTTCCAGGGAGCATGAAACGTTCATCACAGTTCAAGGTTCCTATGGAACAAAGGTTTTCATTCCTACCTAAAGCTTCTTAATCGTCTTTAATTTATGGCTGTGCAGATAGTTTTCCGGTTGCCTAGTCTGATGCTTGAACAATATTTAATCGATTAGGTTCTTGTTCTGTTTGTATACACTTCTTGGTTGAGTTAAGGGCTTAGTCTTAAATTGATGCTTTTGATGTGGCTAGTTGGAGCTTACTCCCTGCAAAAATTGGTTGAGCCTGCTTGATCTTGCAATCTTTTACTGGTTGTAAACGAAAGGCTCCTATCTAACTTATGTTTGAATTCTTGGGTGTCGTGGGGAAGGAAATAGGGAAACATGAGGGTAAGAGAGCCCTAACATGTTTCCTAGATAATAGTCTAGTATAGATGGCTAGGCTGCGTGGAGTGGGTTGCCGGTAGAGAGGGCTAGTTTGGAATTGGGCATTGAATAGTAGAGACACGGTTCCATGTTGTGTTGCAGATTGCATCATTAAATTCAAAATATTGCATCTCATTTCAGTTCTAGTTGTCATGCAGTTGCCTTTGTGTGAGCGAACGACACGTGAGGACATTATCTCCCAACACATGCCTATCTTCATTTTTACCAGTTAATCTAGTTGTTTTATCATTATTTTTGTTCAAAATAAGAACATAGAGAAAACAAAAACTACATCGTGTTAGTCTCTCTATCATTTGTCCATGCCTGTTTTGCAGTCATACTTACTATGTACGACCTTCTGTAGATTGAAGCAGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTCGTTTTCTCTAGTTGGAATGATGTTCTTGATGTTTTGCAATACGCCTTTGCTGCCAACGGTATTACCTTTATCAGGATGAAAGGAGGCAGGTGAGATGATGATAGTATGATAATTTGAATGGCTCAAATTCCGAACAAGAGTGGAAAACTACCTCTATATATTTGTTTTCCCTCATTTCGACCACCTCCATCTTTATGGTTCCTAGTTCGTCTGCATTATCCATTAATATTGATGATTGGTCTTTGCAAATGCAGAAAATCACAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATCACAAGAAACGGGCACAGGTGCCAGAGTTAAAATCCGCTCAAGTTTTATTGCTCTTGATCCAGCATGGAGCTAATGGCCTCAACCTTCTAGAAGCTCAACATGTTGTCCTTGTTGAACCACTGCTCAATCCAGCTGCTGAAGCACAAGCAATTAGCCGAGTACACCGAATTGGACAGGAAAATAAGACGTTCGTTCACCGGTTTATAGTATGTACCTAATTTTCTCTTTTTACAGTTATGCATATTATTATGTACATATAGAAACGATCCATCATGATGCACTCATCTTCAGCCCCTCATCAAAACTCGGTGTTTCATACATGTCAAAGAAAAATCTTTAGCCTAAATGATTAGACTACTACAATCTTCACTAAGAAATATCTAAATATTTTCTTCTCACTCTTTTGGGGTCTTTGTAGGTTAAAGACACAGTGGAAGAGAGCATACACAAGCTAAACAGAAGCAGAGAGAACTCCTCCTTCATCACTGGAAATGCCAAGAACCAAGACCAGCCATTTTTGACGCTTAAAGAAGTTGAATCTCTGTTTGCGTCCAAAGCATCACCATTAGGGGACAGTGATGATAAGGAAAGTGAAACGTTGAGGCATTTGGCACCTTCCATGGCCGCTGCTATGGCAGCTGAAAGGAGACTCAGAGAGCATACAGCG

mRNA sequence

ATGGGTAGAAGGAAGCAAAGTCGACCTCATCGCTCTGGTGGAATAAGACTTGAAATTCATGCGAATCCCGAGACACAGGTTGATATAAATGTGGTTTTGGAAACTGATAAATCATCAGACATCAAACTTAATGAGATTAATGAGCCCTTTTACGTTGAAGTTGATCCGACTGGGTGGAAGTCCAATAAGCACCTTGATATTTCTGAAGTTGTTCTAATGGATTTGAGGTTTGAACACCATTTTGTCGGTTTTCAATTTGATGATAGTTTTTATGAGAGCTCGAGGTACTCATTAAGGTTTCGGCTGTGCAATGTAAATGAGTTTCTCCTTGGTCGTATTAAGTTTGGTCATTGGCCAATTTTATCTTCAAGTGATATATCTTTGGAACTCATCGATAAAGGCATGGAAGAAGGTACGGATGCTTGCTCAGTGGTTTTATCAGGGAACTTTGATGGACCAGATGATGCCATTTCTGGCCTTGTTCATCTTGCAAATTTGAAGTTCATGACATTAAGGCCAGTTATGGGTGCTACCCTTTCCCGTAGTACAGGATCTCTCAGGTTGAGGGTGGAAATGTTATCGAGTGCCCTTGATGCTTGTGAGTCAATATTTGATAACGGCAGGCAGTTGTGGAAAAAGAGTATGATGAGTACCATTGCTTGGTTGCGTCCTGAAGTAGAATCATCTGAGGCCAAGTATGGACTTGTTAAATCAACTAATGTGGACAGTCATTCGCTTCATGAGATGGGGGATGGCTCGTCCAACACTAGGAAACACATAAATTTCGATACAGTTGGCTTTTATGATGCCATTAAGCCATCAAAAGATGATCCTATGCTTGATGAAGACATACCCAATTTGCTTCCAAAGCTAAGACCATATCAACGTCGTGCTGCCTATTGGATGGTACAACGGGAGAAAAGATTTTCTGGAAACTCAGGTGTGGGGGAAAACGTGCAGCTTATTTCTCCCTTGTGTATAGCCCTGGAATGTCTTGATACTTGTACGAGAATGTTTTATAATCCTTTCAGTGGAAATCTCACTTTGAGTGCAGAGAAAACTTTGCCGCACGTTTACGGGGGAATTCTAGCTGATGAGATGGGTCTAGGAAAAACTATTGAACTACTTGCATGCATACTTTCTCACCGGATGTCAGTGTTTGAAGGTGAAAAAGGTTTTGGTAATGAGTCACAACAAATTGTTGGAGGCCAGAGAACTGAATTTAAAAGATTGAAAAGGGAGCGTGTTGAGTGTGTATGTGGAGCTGTTAGTGAAAATCACAGGTACAAGGGGTTGTGGGTACAATGTGACATCTGTGATGCATGGCAACATGCTGACTGCGTTGGTTATTCACCTAAAGGAAGAGTTCTCAAACCCATTGATACAGAGGGTGGGAACTGTATAAAGAAAAAGAGAAACAAAAAAAATAGAGTAAATGTAGTCGTTAGAGATGGGCAACATGTTTGTTTGCCATGCTTAGAGCTGATGCAAGCTACTGACACCCCAATGGCTACAGTTGCAACTCTAATTGTCTGTCCAGCTCCCATATTATTCCAATGGCATGCTGAGATTTTACGTCATACTCGTCCAGGTTCAATAAAACTACTTGTCTACGAAGGAGTGAGGGATACTTCTCTTTCTAGCACATTGTCGGTGCAGATTAACGATCTCGTCAATTCTGATATAATTTTGACATCATATGATGTTCTTAAAGAGGACCTATCCCATGATTCTGATAGACACGAAGGTGATCGACGATTCATGAGATTTCAGAAGAGGTACCCTGTTATCCCTACTCCTCTCACCAGGATCTTTTGGTGGAGGATCTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCTACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCATCGCTGGTGCATTACAGGAACTCCCATTCAAAGGAAACTTGAAGATTTATATGGATTACTAAGATTTGTGAAAGCAAGTCCATTTGATGTTTATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGCTCTTTAAAAATACATGTTACGGATGAATTGCAGCTACCTCCTCAGGAGGAGCAAGTCACTTGGCTAACGTTTTCACCAATTGAAGAGCACTTCTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAGGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCGCAGATTGTGTCTCTTCTGATGCACCATCCGATATTCTCATCACCCATACCGATGCTGGAAAGCTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCACAAGTAGGAAGTTCGGGACTGCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATACTGATGGTTCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCGGTTGTTGCTTTAAATGCACTTGCTGGGATAGCTATAATTGAGAAGAAATTTTCTGAAGCCGTCTCACTATATAAAGAAGCTCTAGAATTGGCTGAGGGGAACAATGAAGATTTTCGCTTAGATCCTTTGTTGAGCATCCACATCCATCACAATCTTGCTGAGATACTACCATCAGCTGTGGACCAGTCGCAATCTCCTTTAAAGGATCAATCTTATTCAAGAACTTGTGAAGTGAAAGCCTCCAAGATGGATGATTCTGAAAATTATGATAATCATACCATGAAGAAACAAAAAGTTAGTGAGACCTCGTATGCTCCTTGTAGTGATACAGAGAAGATATTAGACAATCCTCTTAAGCCTCATCGGTCAAGTAGTTACTTCAATGATAGTTCTTTTAGAAAAGCATGTGAGACTATGAGGCAGAAATATCTAGCTGTCTTTTCCTCAAAGCTATCTATTGCCCAACAAGAGTTCACAAAATCCCACATGCAGGTATGTTTCTACGTGCTACTCCTTTACTCTAGGCTGGAAAATCTTAACAAAGTTTGGTGGCTGGAAGCAGTTGATCATGCTGAACAGAACAAGGATTTCTATCATGAGTTGATAAGAAAGATTGAAGAGGCCGTATCTGGAAACATTAACAACTCGAGATCATCTAGAGTGGGATCCCGTTTCCGCAGCATTAATGCTTTAAAGTATCATGTTCAGACTGGTTTGGATCTTTTGGAAGCTTCTAGGAATGTTGTGCTTGATCGACTTTTAGAAATTGATCAGACGATGGAAAATCCAAAAGAAGAAGATATTGAACGTGTTAGGTATTGTCAAAATTGCCAGGCTGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGACGAACTATTTCAGGAATATGAGGCAAGGCTCTTCAGGCTTAATAAAGTACAAGGAGGAATGGTTTCTTCTGTTGAAGAGGCAGTTGAAGCCCAGAAGAAGAAGTCTGCACTTAATCGTTTCTATTGGAGTCTGTTGCAGCAAAATAAAAATTTAAGTTCATCTAGAGTTGGATATGAAGAATCTAACAAAAGAGATGTTGGAGAAAAAGTTATGGTGTCTAAGCATCCTTCTGAATTGGAGGTCGTTCTTGGTGTTATCAAGAACTTCTGTAAAACACAACTAGGGAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACATCTTCTGGAGTACATGCGAAAGGAGTATGGACATGCAAGGTCCTTAGCCATTGCTCAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTATGCTTAAGGGACAATGACGATGACGACTCAGCTTATGCGATTAGTGAACATGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCTTGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGGTTTGGTGCAATCTAAACAAAACACACCATTAGGTAGTTCTAGTAATCTAGCGTTAACTCAAGAGCCGGCTATCATGTCAACATCTATGGAACAAAAAAGTGAGAACACACCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAATTAAGCAATCAAAAGATGGTTTTTCAATGTGGACATATGATATGCTGTAAATGTTTGTTCGCAATGACTGAAAAGACCCTGCAGGATAGTAAAATTCAAACTCAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGTAATATTGCTTTTGCGGATGATAGTAAAAATGAAATACTTGATCCTTCAACATTGCACGAAACTTCCAGGGAGCATGAAACGTTCATCACAGTTCAAGGTTCCTATGGAACAAAGATTGAAGCAGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTCGTTTTCTCTAGTTGGAATGATGTTCTTGATGTTTTGCAATACGCCTTTGCTGCCAACGGTATTACCTTTATCAGGATGAAAGGAGGCAGAAAATCACAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATCACAAGAAACGGGCACAGGTGCCAGAGTTAAAATCCGCTCAAGTTTTATTGCTCTTGATCCAGCATGGAGCTAATGGCCTCAACCTTCTAGAAGCTCAACATGTTGTCCTTGTTGAACCACTGCTCAATCCAGCTGCTGAAGCACAAGCAATTAGCCGAGTACACCGAATTGGACAGGAAAATAAGACGTTCGTTCACCGGTTTATAGTTAAAGACACAGTGGAAGAGAGCATACACAAGCTAAACAGAAGCAGAGAGAACTCCTCCTTCATCACTGGAAATGCCAAGAACCAAGACCAGCCATTTTTGACGCTTAAAGAAGTTGAATCTCTGTTTGCGTCCAAAGCATCACCATTAGGGGACAGTGATGATAAGGAAAGTGAAACGTTGAGGCATTTGGCACCTTCCATGGCCGCTGCTATGGCAGCTGAAAGGAGACTCAGAGAGCATACAGCG

Coding sequence (CDS)

ATGGGTAGAAGGAAGCAAAGTCGACCTCATCGCTCTGGTGGAATAAGACTTGAAATTCATGCGAATCCCGAGACACAGGTTGATATAAATGTGGTTTTGGAAACTGATAAATCATCAGACATCAAACTTAATGAGATTAATGAGCCCTTTTACGTTGAAGTTGATCCGACTGGGTGGAAGTCCAATAAGCACCTTGATATTTCTGAAGTTGTTCTAATGGATTTGAGGTTTGAACACCATTTTGTCGGTTTTCAATTTGATGATAGTTTTTATGAGAGCTCGAGGTACTCATTAAGGTTTCGGCTGTGCAATGTAAATGAGTTTCTCCTTGGTCGTATTAAGTTTGGTCATTGGCCAATTTTATCTTCAAGTGATATATCTTTGGAACTCATCGATAAAGGCATGGAAGAAGGTACGGATGCTTGCTCAGTGGTTTTATCAGGGAACTTTGATGGACCAGATGATGCCATTTCTGGCCTTGTTCATCTTGCAAATTTGAAGTTCATGACATTAAGGCCAGTTATGGGTGCTACCCTTTCCCGTAGTACAGGATCTCTCAGGTTGAGGGTGGAAATGTTATCGAGTGCCCTTGATGCTTGTGAGTCAATATTTGATAACGGCAGGCAGTTGTGGAAAAAGAGTATGATGAGTACCATTGCTTGGTTGCGTCCTGAAGTAGAATCATCTGAGGCCAAGTATGGACTTGTTAAATCAACTAATGTGGACAGTCATTCGCTTCATGAGATGGGGGATGGCTCGTCCAACACTAGGAAACACATAAATTTCGATACAGTTGGCTTTTATGATGCCATTAAGCCATCAAAAGATGATCCTATGCTTGATGAAGACATACCCAATTTGCTTCCAAAGCTAAGACCATATCAACGTCGTGCTGCCTATTGGATGGTACAACGGGAGAAAAGATTTTCTGGAAACTCAGGTGTGGGGGAAAACGTGCAGCTTATTTCTCCCTTGTGTATAGCCCTGGAATGTCTTGATACTTGTACGAGAATGTTTTATAATCCTTTCAGTGGAAATCTCACTTTGAGTGCAGAGAAAACTTTGCCGCACGTTTACGGGGGAATTCTAGCTGATGAGATGGGTCTAGGAAAAACTATTGAACTACTTGCATGCATACTTTCTCACCGGATGTCAGTGTTTGAAGGTGAAAAAGGTTTTGGTAATGAGTCACAACAAATTGTTGGAGGCCAGAGAACTGAATTTAAAAGATTGAAAAGGGAGCGTGTTGAGTGTGTATGTGGAGCTGTTAGTGAAAATCACAGGTACAAGGGGTTGTGGGTACAATGTGACATCTGTGATGCATGGCAACATGCTGACTGCGTTGGTTATTCACCTAAAGGAAGAGTTCTCAAACCCATTGATACAGAGGGTGGGAACTGTATAAAGAAAAAGAGAAACAAAAAAAATAGAGTAAATGTAGTCGTTAGAGATGGGCAACATGTTTGTTTGCCATGCTTAGAGCTGATGCAAGCTACTGACACCCCAATGGCTACAGTTGCAACTCTAATTGTCTGTCCAGCTCCCATATTATTCCAATGGCATGCTGAGATTTTACGTCATACTCGTCCAGGTTCAATAAAACTACTTGTCTACGAAGGAGTGAGGGATACTTCTCTTTCTAGCACATTGTCGGTGCAGATTAACGATCTCGTCAATTCTGATATAATTTTGACATCATATGATGTTCTTAAAGAGGACCTATCCCATGATTCTGATAGACACGAAGGTGATCGACGATTCATGAGATTTCAGAAGAGGTACCCTGTTATCCCTACTCCTCTCACCAGGATCTTTTGGTGGAGGATCTGTTTGGATGAGGGACAAATGGTAGAGAGCAATGCTACTGCTGCTACTGAAATGGCTTCACGACTTTATGCTAGTCATCGCTGGTGCATTACAGGAACTCCCATTCAAAGGAAACTTGAAGATTTATATGGATTACTAAGATTTGTGAAAGCAAGTCCATTTGATGTTTATAGATGGTGGGTTGAAGTTATAAGAGATCCATATGAGAGGAGGGATCCTGGTGCTATGGAATTCACACACAAATTCTTTAAGCAAATAATGTGGCGCTCTTTAAAAATACATGTTACGGATGAATTGCAGCTACCTCCTCAGGAGGAGCAAGTCACTTGGCTAACGTTTTCACCAATTGAAGAGCACTTCTACCAGAGGCAGCATGAAACTTGTGTGAGTTATGCCCGTGAAGTTATTCAGGGTTTGAAAGATGATTTTGTCAAAAGGAAAGTCGCAGATTGTGTCTCTTCTGATGCACCATCCGATATTCTCATCACCCATACCGATGCTGGAAAGCTGCTGAGCACACTTTTGAAACTTCGCCAAGCTTGCTGTCACCCACAAGTAGGAAGTTCGGGACTGCGCTCATTGCAACAATCCCCAATGACAATGGAGGAAATACTGATGGTTCTTGTTAGTAAGACCAAGATAGAAGGAGAGGAAGCTCTGAGGAGGTCGGTTGTTGCTTTAAATGCACTTGCTGGGATAGCTATAATTGAGAAGAAATTTTCTGAAGCCGTCTCACTATATAAAGAAGCTCTAGAATTGGCTGAGGGGAACAATGAAGATTTTCGCTTAGATCCTTTGTTGAGCATCCACATCCATCACAATCTTGCTGAGATACTACCATCAGCTGTGGACCAGTCGCAATCTCCTTTAAAGGATCAATCTTATTCAAGAACTTGTGAAGTGAAAGCCTCCAAGATGGATGATTCTGAAAATTATGATAATCATACCATGAAGAAACAAAAAGTTAGTGAGACCTCGTATGCTCCTTGTAGTGATACAGAGAAGATATTAGACAATCCTCTTAAGCCTCATCGGTCAAGTAGTTACTTCAATGATAGTTCTTTTAGAAAAGCATGTGAGACTATGAGGCAGAAATATCTAGCTGTCTTTTCCTCAAAGCTATCTATTGCCCAACAAGAGTTCACAAAATCCCACATGCAGGTATGTTTCTACGTGCTACTCCTTTACTCTAGGCTGGAAAATCTTAACAAAGTTTGGTGGCTGGAAGCAGTTGATCATGCTGAACAGAACAAGGATTTCTATCATGAGTTGATAAGAAAGATTGAAGAGGCCGTATCTGGAAACATTAACAACTCGAGATCATCTAGAGTGGGATCCCGTTTCCGCAGCATTAATGCTTTAAAGTATCATGTTCAGACTGGTTTGGATCTTTTGGAAGCTTCTAGGAATGTTGTGCTTGATCGACTTTTAGAAATTGATCAGACGATGGAAAATCCAAAAGAAGAAGATATTGAACGTGTTAGGTATTGTCAAAATTGCCAGGCTGACAGTAATGGACCACCTTGTGTTCTTTGTGAACTAGACGAACTATTTCAGGAATATGAGGCAAGGCTCTTCAGGCTTAATAAAGTACAAGGAGGAATGGTTTCTTCTGTTGAAGAGGCAGTTGAAGCCCAGAAGAAGAAGTCTGCACTTAATCGTTTCTATTGGAGTCTGTTGCAGCAAAATAAAAATTTAAGTTCATCTAGAGTTGGATATGAAGAATCTAACAAAAGAGATGTTGGAGAAAAAGTTATGGTGTCTAAGCATCCTTCTGAATTGGAGGTCGTTCTTGGTGTTATCAAGAACTTCTGTAAAACACAACTAGGGAAAGAAAGTATAGCAGCAGCCAACAAGCAGCTACATCTTCTGGAGTACATGCGAAAGGAGTATGGACATGCAAGGTCCTTAGCCATTGCTCAAGCTCAAGTTTTGAATGCTCATGACGAAATTAAGATGGCAACCACGAGATTATGCTTAAGGGACAATGACGATGACGACTCAGCTTATGCGATTAGTGAACATGAGCTACCTGCAGCTAGTGTCCAGTATTCTAGCGATAAGTTCTTGTCCTTGGCTATGTTATCACGTGTAAAAGGAAAACTTCGTTATTTGAAGGGTTTGGTGCAATCTAAACAAAACACACCATTAGGTAGTTCTAGTAATCTAGCGTTAACTCAAGAGCCGGCTATCATGTCAACATCTATGGAACAAAAAAGTGAGAACACACCGAAAGCTGATGAGGAATCATGCCCTGTTTGCCAAGAAAAATTAAGCAATCAAAAGATGGTTTTTCAATGTGGACATATGATATGCTGTAAATGTTTGTTCGCAATGACTGAAAAGACCCTGCAGGATAGTAAAATTCAAACTCAATGGGTGATGTGCCCAACCTGTCGCCAACACACAGATTTTGGTAATATTGCTTTTGCGGATGATAGTAAAAATGAAATACTTGATCCTTCAACATTGCACGAAACTTCCAGGGAGCATGAAACGTTCATCACAGTTCAAGGTTCCTATGGAACAAAGATTGAAGCAGTTGTAAGACGAATCTTGTGGATCAAGTATACAGATTCCAAAGCAAAAGTTCTCGTTTTCTCTAGTTGGAATGATGTTCTTGATGTTTTGCAATACGCCTTTGCTGCCAACGGTATTACCTTTATCAGGATGAAAGGAGGCAGAAAATCACAAACAGCCATTAGTGAATTTAGAGGACAGAAGATCAATGCAAAAGAAAATCACAAGAAACGGGCACAGGTGCCAGAGTTAAAATCCGCTCAAGTTTTATTGCTCTTGATCCAGCATGGAGCTAATGGCCTCAACCTTCTAGAAGCTCAACATGTTGTCCTTGTTGAACCACTGCTCAATCCAGCTGCTGAAGCACAAGCAATTAGCCGAGTACACCGAATTGGACAGGAAAATAAGACGTTCGTTCACCGGTTTATAGTTAAAGACACAGTGGAAGAGAGCATACACAAGCTAAACAGAAGCAGAGAGAACTCCTCCTTCATCACTGGAAATGCCAAGAACCAAGACCAGCCATTTTTGACGCTTAAAGAAGTTGAATCTCTGTTTGCGTCCAAAGCATCACCATTAGGGGACAGTGATGATAAGGAAAGTGAAACGTTGAGGCATTTGGCACCTTCCATGGCCGCTGCTATGGCAGCTGAAAGGAGACTCAGAGAGCATACAGCG

Protein sequence

MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWKSNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLSRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTNVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA
Homology
BLAST of MS003347 vs. NCBI nr
Match: XP_022134046.1 (E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiquitin-protein ligase SHPRH [Momordica charantia])

HSP 1 Score: 3293.8 bits (8539), Expect = 0.0e+00
Identity = 1659/1675 (99.04%), Postives = 1664/1675 (99.34%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQSRPHRSGGIRLEIHANPETQVDI+VVLET+KSSDIKLNEINEPFYVEVDPTGWK
Sbjct: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI
Sbjct: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS
Sbjct: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN
Sbjct: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG
Sbjct: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVL+PIDTEGGNCIKKKRNKKNRVNV
Sbjct: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
            VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG
Sbjct: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL
Sbjct: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA SLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK
Sbjct: 841  GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
            DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN
Sbjct: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960

Query: 961  DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLNKVWWLEAV 1020
            DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVC  +       E+LNKVWWLEAV
Sbjct: 961  DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSEL----RERESLNKVWWLEAV 1020

Query: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
            DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV
Sbjct: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080

Query: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
            VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV
Sbjct: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140

Query: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHP 1200
            QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMV KHP
Sbjct: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVCKHP 1200

Query: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
            SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI
Sbjct: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260

Query: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
            KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS
Sbjct: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320

Query: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
            KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI
Sbjct: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380

Query: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
            CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH
Sbjct: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440

Query: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
            ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM
Sbjct: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500

Query: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
            KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL
Sbjct: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560

Query: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
            VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN
Sbjct: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620

Query: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1676
            QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA
Sbjct: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1671

BLAST of MS003347 vs. NCBI nr
Match: XP_038884903.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida])

HSP 1 Score: 2867.0 bits (7431), Expect = 0.0e+00
Identity = 1462/1698 (86.10%), Postives = 1551/1698 (91.34%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   +P+TQ+DIN  +E   SSDIK+ E+NEP +VEVD TGW 
Sbjct: 1    MGRRKQRQPHRSGGIRFEDRGDPKTQIDINGAVE---SSDIKVAEVNEPIFVEVDRTGWN 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEV LMD+R EH FV F+ D SF E+SRYSLRFRLCNVN  LL RIKFGHWP+
Sbjct: 61   SNEHHDISEVFLMDVRLEHPFVDFRLDKSFCENSRYSLRFRLCNVNGSLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS+DI LELI++ MEE   ACSVVLSGNFDGPD+AISGLVHLANLKF TLRPV G ++S
Sbjct: 121  LSSNDILLELIERDMEEDMKACSVVLSGNFDGPDEAISGLVHLANLKFATLRPVEGVSVS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++TGSLRLRVE+LSSA  ACESIFDNGRQLWKKSMM+TIAWLRPEV  SE KYG+VKSTN
Sbjct: 181  QNTGSLRLRVEILSSAFAACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +D+   H  GD +SN+RKH+NFDTV FYDAIKPSKD+PMLDEDIPNLLPKLR YQRRAAY
Sbjct: 241  MDTSLHHGTGDDTSNSRKHVNFDTVAFYDAIKPSKDEPMLDEDIPNLLPKLRQYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FSGN GVGENVQLISPLC+AL+C+DTC+R+FYNPFSGNLTL+AE+TLPHVYG
Sbjct: 301  WMVQREKGFSGNLGVGENVQLISPLCMALKCVDTCSRVFYNPFSGNLTLNAEQTLPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSH+MSVFEG KGF +E QQ V  QRTEFKRLKRERVEC+C
Sbjct: 361  GILADEMGLGKTIELLACILSHQMSVFEGGKGFHDELQQPVEDQRTEFKRLKRERVECLC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGR+LKPIDTEGGNC K+KRN +N +NV
Sbjct: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRILKPIDTEGGNCRKEKRNNRNTLNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
            +VR  +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHT PGSIKLLVYEG
Sbjct: 481  IVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSL STLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541  VRDTSLLSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK+KV DC+SSD PSD+LI H DAGKLL
Sbjct: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKKKVPDCISSDIPSDLLINHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA SLYKEALELAE NNEDFRLDPLLSIHIHHNLAEILP AVDQSQS LK
Sbjct: 841  GIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVDQSQSRLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDN--HTMKKQKVSETSYAPCSD--TEKILDNPL------ 960
            DQ   RT EVKA++MDDSE YDN  H MKKQKVSET YAPCS+  T K++D PL      
Sbjct: 901  DQFCPRTSEVKAARMDDSEKYDNHVHVMKKQKVSETLYAPCSEDNTGKMIDRPLELTRKD 960

Query: 961  ---------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYV 1020
                     +PH SSSYF+D S RKACE MRQKYLAVFSSKLS+AQQEFTKS+MQV    
Sbjct: 961  TNTKKEENCEPHLSSSYFDDISIRKACEAMRQKYLAVFSSKLSVAQQEFTKSYMQV---- 1020

Query: 1021 LLLYSRLE---NLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFR 1080
                S L+   NLN VWWLEAV HAEQNKDF HELIRK+EEAVSGN+NNS+ SRVGSRFR
Sbjct: 1021 ---GSELKDRGNLNHVWWLEAVHHAEQNKDFSHELIRKVEEAVSGNLNNSK-SRVGSRFR 1080

Query: 1081 SINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPC 1140
            SINALKYHVQ+GLDLLEASR VVLDRLLEIDQTMENPKEEDIERVRYC+NCQADSNGPPC
Sbjct: 1081 SINALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPC 1140

Query: 1141 VLCELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSS 1200
            VLCELD+LFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKN SSS
Sbjct: 1141 VLCELDDLFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSS 1200

Query: 1201 RVGYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK 1260
            RVGYEE NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK
Sbjct: 1201 RVGYEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRK 1260

Query: 1261 EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKF 1320
            EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRD DDD SAYAISEHELPAASVQYSSDKF
Sbjct: 1261 EYGHARSLAIAQAQVLNAHDEIKMATTRLCLRD-DDDSSAYAISEHELPAASVQYSSDKF 1320

Query: 1321 LSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEE 1380
            +SLAML+RVKGKLRYLKGLVQSKQN PL SSSNLALTQ+PA MSTSMEQKSENT KADEE
Sbjct: 1321 MSLAMLARVKGKLRYLKGLVQSKQNIPLDSSSNLALTQQPATMSTSMEQKSENTSKADEE 1380

Query: 1381 SCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIA 1440
            SCPVCQEKLSNQKMVFQCGH+ CCKCLFAMTEKTL  SKIQT+WVMCPTCRQHTDFGNIA
Sbjct: 1381 SCPVCQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIA 1440

Query: 1441 FADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSS 1500
            +ADDSKNE LDPSTLHETSREHE  ITVQGSYGTK+EAVVRRILWIKYTDSKAKVLVFSS
Sbjct: 1441 YADDSKNETLDPSTLHETSREHELSITVQGSYGTKVEAVVRRILWIKYTDSKAKVLVFSS 1500

Query: 1501 WNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSA 1560
            WNDVLDVLQYAFAAN ITFIRMKGGR+S TAISEFRGQKINAKEN KKR   +VPEL+SA
Sbjct: 1501 WNDVLDVLQYAFAANSITFIRMKGGRQSHTAISEFRGQKINAKENQKKRPSGKVPELRSA 1560

Query: 1561 QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDT 1620
            QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDT
Sbjct: 1561 QVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQKNKTFVHRFIVKDT 1620

Query: 1621 VEESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHL 1675
            VEESI+KLNRSRE+SSFITGN KNQDQP LTLKEVESLFASKASPL ++D+K SETLRHL
Sbjct: 1621 VEESIYKLNRSRESSSFITGNTKNQDQPLLTLKEVESLFASKASPLRENDEKGSETLRHL 1680

BLAST of MS003347 vs. NCBI nr
Match: XP_023551402.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2863.2 bits (7421), Expect = 0.0e+00
Identity = 1445/1691 (85.45%), Postives = 1553/1691 (91.84%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   NP+TQVDI+ ++ET +SSDIKL+E+NEPF+VEVD TGW 
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVNPKTQVDIHGIVETVESSDIKLDEVNEPFFVEVDRTGWH 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEVVLMD+R EH FVG + ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61   SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS++ISLELI++ +EE  + C VVLSG+FDGPD+AISGLVHLANLKF+TLRPVMG + S
Sbjct: 121  LSSNEISLELIERRVEEDMNGCLVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V+STN
Sbjct: 181  QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +D+H   E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+TLPHVYG
Sbjct: 301  WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQTLPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSH MSVFE  KGF +E QQ+VG QRT+FKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHPMSVFEDGKGFDDEMQQLVGDQRTKFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGRVL+P+DT+ GNC K KRNK+N VNV
Sbjct: 421  GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRVLEPVDTKDGNCRKDKRNKRNTVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
             VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481  TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLS+TL VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541  VRDTSLSNTLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL+GLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLFGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV   VSSD PSDILITH DAGKLL
Sbjct: 721  TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            +TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781  NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841  GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
            DQ Y RTCEVKASKMDDSE  DNH +KKQKV+ T Y PCSDTEKI+D+PL          
Sbjct: 901  DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960

Query: 961  -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
                 +PHRSSSYFND+  RKACE MRQKYLAVFSSKLSIAQQEFTKS++QVC     L 
Sbjct: 961  KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCNE---LK 1020

Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
            SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080

Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
            HVQTGLDLLEASR +VL RLLEIDQTMENPK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
            LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
             LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 ALSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
            E LDPSTLH+TSRE E  I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
            LQYAFAAN ITF+RMKGGRKS TAISEFRGQKIN KENHKK+  A+VPEL+SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
            LNRSRE+SSFITGN KNQDQP  TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA 
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

BLAST of MS003347 vs. NCBI nr
Match: XP_022938761.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata])

HSP 1 Score: 2851.2 bits (7390), Expect = 0.0e+00
Identity = 1439/1691 (85.10%), Postives = 1549/1691 (91.60%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   +P+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW 
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61   SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS+DISLELI++ +EE  + CSVVLSG+FDGPD+AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121  LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181  QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +D+H   E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301  WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSHRMSVFE  KG  +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421  GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
             VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481  TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLS+T  VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541  VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV   VSSD PSDILITH DAGKLL
Sbjct: 721  TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            +TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781  NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841  GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
            DQ Y RTCEVKASKMDDSE  DNH +KKQKV+ T YAPCSDTEKI+D+PL          
Sbjct: 901  DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960

Query: 961  -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
                 +PHRSSSYFND+  RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC     L 
Sbjct: 961  KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020

Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
            SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080

Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
            HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
            LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
             LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
            E LDPSTLH+TSRE E  I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
            LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+  A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
            LNRSRE+SSFITGN KNQDQP  TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA 
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

BLAST of MS003347 vs. NCBI nr
Match: KAG6578487.1 (E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2845.8 bits (7376), Expect = 0.0e+00
Identity = 1436/1691 (84.92%), Postives = 1547/1691 (91.48%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   NP+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW 
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVNPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61   SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS+DISLELI++ +EE  + CSVVLSG+FDGP +AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121  LSSNDISLELIERHVEEDMNGCSVVLSGSFDGPYEAISGLVHLAHLKFVTLRPVMGVSFS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181  QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVVSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +D+H   E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301  WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSHR+SVFE  KG  +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHRISVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421  GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
             VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481  TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLS+T  VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541  VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV   VSSD PSDILITH DAGKLL
Sbjct: 721  TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            +TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781  NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841  GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
            DQ Y RTCEVKASKMDDSE  DNH +KKQKV+ T YAPCSDTEKI+D+PL          
Sbjct: 901  DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960

Query: 961  -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
                 +PHRSSSYFND+  RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC     L 
Sbjct: 961  KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020

Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
            SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080

Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
            HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
            LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSSDKF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLATLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
             LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
            E LDPSTLH+TSRE E  I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
            LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+  A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
            LNRSRE+SSFI GN KNQDQP  TLKEVESLFAS+ SPL ++D+KESETLRHLAPS+AA 
Sbjct: 1621 LNRSRESSSFIAGNTKNQDQPVFTLKEVESLFASRPSPLRENDEKESETLRHLAPSLAAT 1680

BLAST of MS003347 vs. ExPASy Swiss-Prot
Match: Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)

HSP 1 Score: 523.1 bits (1346), Expect = 1.2e-146
Identity = 380/1240 (30.65%), Postives = 576/1240 (46.45%), Query Frame = 0

Query: 415  RVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNK 474
            R EC+CG + +  R     VQC  C  WQHA CV Y  K   +KP               
Sbjct: 658  RFECICGELDQIDRKPR--VQCLKCHLWQHAKCVNYDEKNLKIKPF-------------- 717

Query: 475  KNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIK 534
                          C  CL  M+    P++T ATLI+ P+ I  QW  EI RH R  S++
Sbjct: 718  -------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLR 777

Query: 535  LLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEG--DRRFMRFQK 594
            +LVY+GV+         +Q + L   DI++ +YDVL+ +L++    H    D R +R QK
Sbjct: 778  VLVYQGVKKDGF-----LQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQK 837

Query: 595  RYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLY 654
            RY  IP+PL  + WWRICLDE QMVE     A EMA RL   +RWCI+GTP+QR LEDL+
Sbjct: 838  RYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLF 897

Query: 655  GLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPP 714
            GL+ F+   P+ V  WWV ++  PY +++P   +  + F  +I+WRS K  V D++Q+PP
Sbjct: 898  GLVVFLGIEPYCVKHWWVRLLYRPYCKKNP---QHLYSFIAKILWRSAKKDVIDQIQIPP 957

Query: 715  QEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILIT 774
            Q E++ WL FSP+E HFY RQHE C    ++V+  L      RK++D     +  D    
Sbjct: 958  QTEEIHWLHFSPVERHFYHRQHEVC---CQDVVVKL------RKISDWALKLSSLD---- 1017

Query: 775  HTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRS 834
                  +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R+ 
Sbjct: 1018 RRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQL 1077

Query: 835  VVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAV 894
            V ALN LAGI II+ +++ A  LY+E L  +E +    + D L  +H  HNL E+L    
Sbjct: 1078 VCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL---- 1137

Query: 895  DQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKP 954
                                                                I  +P  P
Sbjct: 1138 ----------------------------------------------------IARHPGIP 1197

Query: 955  HRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLN 1014
                    D    +  + +R+ Y++  +++++ AQQ      +Q   + L    R  + N
Sbjct: 1198 ----PTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALYP--VQQTIHEL---QRKIHSN 1257

Query: 1015 KVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS-RVGSRFRSINALKYHVQTGL 1074
              WWL  + H         EL++++   ++ N         +  +FR    L++ + T +
Sbjct: 1258 SPWWLNVI-HRAIEFTIDEELVQRVRNEITSNYKQQTGKLSMSEKFRDCRGLQFLLTTQM 1317

Query: 1075 DLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYE 1134
            + L   + +V + +  ++     P    IE    C    A      CV C+ DELF EYE
Sbjct: 1318 EELNKCQKLVREAVKNLEGP---PSRNVIESATVCHLRPARLPLNCCVFCKADELFTEYE 1377

Query: 1135 ARLFRLNKVQGGMVSSVEEAVEAQK-----KKSALNRFYWSLLQQNKNLSSSRVGYEESN 1194
            ++LF  N V+ G  +  EE +E ++     +     R  W++ +  +++ +  + + +S+
Sbjct: 1378 SKLFS-NTVK-GQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAI-LSFAKSH 1437

Query: 1195 KRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSL 1254
            + DV                              E +   +  + L E  +KEY      
Sbjct: 1438 RFDV------------------------------EFVDEGSTSMDLFEAWKKEYKLLHEY 1497

Query: 1255 AIAQAQVLNAHDEIKMATTRLCLRD----NDDDDSAYAISEHELPAASVQYSSDKFLSLA 1314
             +A    ++A DE+ MAT RL +RD      +    + I  HE+    ++  +DK ++ +
Sbjct: 1498 WMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATS 1557

Query: 1315 MLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPV 1374
             L +  G+L YL  L +S+  T  G                            + E CP+
Sbjct: 1558 QLQKKLGQLLYLTNLEKSQDKTSGG---------------------------VNPEPCPI 1617

Query: 1375 CQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADD 1434
            C  +L  Q  V  CGH  C +C+  + E+    S   +  + C  CRQ T    I++   
Sbjct: 1618 CARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSS--IKCAICRQTTSHKEISYVFT 1677

Query: 1435 SKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDV 1494
            S+                E  I V+GS+ TK+EAVVR ++ I+  D  AK LVFS+W DV
Sbjct: 1678 SE------------KANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDV 1682

Query: 1495 LDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLL 1554
            LD++  A   N + F ++   +  Q  +S F           K+  Q+       +LLL 
Sbjct: 1738 LDIISKALTDNNMEFAQISRVKTFQENLSAF-----------KRDPQI------NILLLP 1682

Query: 1555 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIH 1614
            +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ   T VHRF++K T+EE + 
Sbjct: 1798 LHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQ 1682

Query: 1615 KLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1643
             + ++ E  S    +AK+ +   LT+ ++  LF  +   L
Sbjct: 1858 AMLKTAER-SHTNSSAKHSEASVLTVADLADLFTKETEEL 1682


HSP 2 Score: 80.9 bits (198), Expect = 1.5e-13
Identity = 56/172 (32.56%), Postives = 90/172 (52.33%), Query Frame = 0

Query: 280 LDEDIPNLLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMF 339
           +D   P L+P LRPYQR A  WM+Q+E  F  +      +  +    +  E L    +++
Sbjct: 293 VDVQHPALIPVLRPYQREAVNWMLQQE-CFRSSPATESALHFLWREIVTSEGL----KLY 352

Query: 340 YNPFSGNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSH--------RMSVFEGEK 399
           YNP++G +      + P + GGILADEMGLGKT+E+LA IL+H         +++ EG+ 
Sbjct: 353 YNPYTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKV 412

Query: 400 GFGNESQQIVGG--QRTEFKRLK---RERVEC------VCGAVSENHRYKGL 433
                     GG  ++TE + ++   +E+V+C      +  AV E +  KG+
Sbjct: 413 VNYFIPSHYFGGKLKKTEIQNIEFEPKEKVQCPPTRVMILTAVKEMNGKKGV 459

BLAST of MS003347 vs. ExPASy Swiss-Prot
Match: Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 3.2e-144
Identity = 375/1242 (30.19%), Postives = 572/1242 (46.05%), Query Frame = 0

Query: 415  RVECVCGAVSE-NHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRN 474
            R EC+CG   +  H+ +   VQC  C  WQHA CV Y  K   +KP              
Sbjct: 649  RFECICGEFDQIGHKPR---VQCLKCHLWQHAKCVNYEEKNLKVKPF------------- 708

Query: 475  KKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSI 534
                           C  CL  M+    P++T ATLI+ P+ I  QW  EI RH R  S+
Sbjct: 709  --------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSL 768

Query: 535  KLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEG--DRRFMRFQ 594
            ++LVY+GV+         +Q + L   DI++ +YDVL+ +L++ +  H    D R +R Q
Sbjct: 769  RVLVYQGVKKHGF-----LQPHFLAEQDIVIITYDVLRSELNYVNIPHSNSEDGRRLRNQ 828

Query: 595  KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 654
            KRY  IP+PL  + WWRICLDE QMVE     A EMA RL   +RWCI+GTP+QR LEDL
Sbjct: 829  KRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDL 888

Query: 655  YGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLP 714
            +GL+ F+   P+ V  WW+ ++  PY +++P   +  + F  +IMWRS K  V D++Q+P
Sbjct: 889  FGLVVFLGIEPYCVKHWWIRLLYHPYCKKNP---QHLYSFIAKIMWRSAKKDVIDQIQIP 948

Query: 715  PQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVK-RKVADCVSSDAPSDIL 774
            PQ E++ WL FSP+E HFY RQHE C           +D  VK RK++D     +  D  
Sbjct: 949  PQTEEMHWLHFSPVERHFYHRQHEVC----------CQDAIVKLRKISDWALKLSSLD-- 1008

Query: 775  ITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALR 834
                    +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R
Sbjct: 1009 --RRTVSSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHR 1068

Query: 835  RSVVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPS 894
            + V ALN LAGI II+ +++ A  LY+E L  +E +    + D L  +H  HNL E+L +
Sbjct: 1069 QLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLGA 1128

Query: 895  AVDQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL 954
                                                K   +  T                
Sbjct: 1129 ------------------------------------KHPGIPPT---------------- 1188

Query: 955  KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLEN 1014
                      D    +  + +R+ Y++  +++++ AQQ        +      ++S    
Sbjct: 1189 --------LRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQSIRELQRKIHS---- 1248

Query: 1015 LNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS-RVGSRFRSINALKYHVQT 1074
             N  WWL  + H         EL++++   +S N         +  +FR    L++ + T
Sbjct: 1249 -NSPWWLNVI-HRAMEFSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRDCRGLQFLLTT 1308

Query: 1075 GLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQE 1134
             ++ L   + +V + + ++++    P  E IE    C    A      CV C+ DELF E
Sbjct: 1309 QMEELHKFQKLVREAVKKLEKP---PSREVIESATVCHLRPARLPLNCCVFCKADELFTE 1368

Query: 1135 YEARLFRLNKVQGGMVSSVEEAVEAQK-----KKSALNRFYWSLLQQNKNLSSSRVGYEE 1194
            YE++LF  N V+ G  +  EE +E ++     +     R  W++ +  +++ +  + +  
Sbjct: 1369 YESKLF-FNTVK-GQTAIFEEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAI-LSFAR 1428

Query: 1195 SNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHAR 1254
            S++ DV                              E +   +  + L E  +KEY    
Sbjct: 1429 SHRFDV------------------------------EYVDEGSVSMDLFEAWKKEYKLLH 1488

Query: 1255 SLAIAQAQVLNAHDEIKMATTRLCLR----DNDDDDSAYAISEHELPAASVQYSSDKFLS 1314
               +     ++A DE+ MAT RL +R       +    + I  HE+    ++  +DK ++
Sbjct: 1489 EYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEVEQNRIKLVNDKAVA 1548

Query: 1315 LAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESC 1374
             + L +  G+L YL  L +S+  T  G                            + E C
Sbjct: 1549 TSQLQKKLGQLLYLTNLEKSQDKTSGG---------------------------INPEPC 1608

Query: 1375 PVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFA 1434
            P+C  +L  Q  V  CGH  C +C   + E+    S   +  + C  CRQ T    +++ 
Sbjct: 1609 PICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSS--IKCAICRQTTSHKEVSYV 1668

Query: 1435 DDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWN 1494
              S+                E  I V+GS+ TK+EAVVR ++ I+  D  AK LVFS+W 
Sbjct: 1669 FTSE------------KANQEDDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQ 1673

Query: 1495 DVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLL 1554
            DVLD++  A   N + F ++   +  Q  +S F+                P +    +LL
Sbjct: 1729 DVLDIISKALTDNNMEFTQISRIKTFQENLSAFKYD--------------PHI---NILL 1673

Query: 1555 LLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEES 1614
            L +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ   T VHRF++K T+EE 
Sbjct: 1789 LPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEER 1673

Query: 1615 IHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPL 1643
            +  + ++ E  S  + + K+ +   LT+  +  LF  +   L
Sbjct: 1849 MQAMLKTAER-SHTSSSGKHSEASVLTVAGLADLFTKENEEL 1673


HSP 2 Score: 78.6 bits (192), Expect = 7.6e-13
Identity = 41/103 (39.81%), Postives = 61/103 (59.22%), Query Frame = 0

Query: 280 LDEDIPNLLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMF 339
           +D   P L+P LRPYQR A  WM+Q+E +F        ++  +    +  + L    +++
Sbjct: 288 VDVQHPALIPVLRPYQREAVNWMLQQE-QFRSAPPADNSLHFLWREIVTPDGL----KLY 347

Query: 340 YNPFSGNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSH 383
           YNP++G +        P + GGILADEMGLGKT+E+LA IL+H
Sbjct: 348 YNPYTGCIIRDFPHAGPQLLGGILADEMGLGKTVEVLALILTH 385

BLAST of MS003347 vs. ExPASy Swiss-Prot
Match: Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 6.4e-60
Identity = 316/1368 (23.10%), Postives = 553/1368 (40.42%), Query Frame = 0

Query: 285  PNLLPKLRPYQRRAAYWMVQR--EKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNP 344
            P L  +L P+Q R   WM +R  EK  + N         + PL    + L     ++ N 
Sbjct: 212  PRLSSELLPFQMRVLEWMKRREEEKFLTSND--------LPPLWYHCKSLFDDRMVYVNH 271

Query: 345  FSGNLTLSAEKTL----PHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQ 404
              G +T S EKT       + GGILADEMG+GKT+E+L  +L H++ +            
Sbjct: 272  VYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPI------------ 331

Query: 405  QIVGGQRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLK 464
                                                   + D       VG + K     
Sbjct: 332  --------------------------------------SLTDTCTFDQVVGKNVK----- 391

Query: 465  PIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILF 524
                                                          + ATLI+ P+ IL 
Sbjct: 392  ---------------------------------------------YSKATLIITPSTILD 451

Query: 525  QWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDS 584
            QW +EI  H    S+K+  Y+G+R ++   +  +     ++ DI++TSY  L+ +L +  
Sbjct: 452  QWLSEIDLHV--PSLKVFHYQGIRKSNGLKSAKI----FLDCDIVVTSYSDLRFELLYT- 511

Query: 585  DRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWC 644
               E   R +R +KR+    +PL  + WWRIC+DE QMVE++ +   +M  R+   + W 
Sbjct: 512  ---ESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWT 571

Query: 645  ITGTPIQRKLEDLYGLLRFVKASPFDVYR--WWVEVIRDPYERRDPGAMEFTHKFFKQIM 704
            ++GTP++ +++DL+GLL  ++ SP  +Y+   W+++I     R      EF    F  ++
Sbjct: 572  VSGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVR------EFC-DLFGSLV 631

Query: 705  WRSLKIHVTDELQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRK 764
             R  K  V +EL+LPPQ         S +EE  YQ      +S A + +   KD    R 
Sbjct: 632  CRHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQ----DLLSEAAKSLHFFKD----RN 691

Query: 765  VADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM-TMEEILM 824
            +  C              D   +   L++LRQACCHPQVG     +    PM ++ ++L+
Sbjct: 692  LDLC--------------DEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLV 751

Query: 825  VLVSKTKIEGEEALRRSVVALNALAG-IAIIEKKFSEAVSLYKEA---LELAEGNNEDFR 884
             ++ +T      +L R + +   + G I    K +++A++++ E    +ELA    E+  
Sbjct: 752  FMLEQTN-STFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENV- 811

Query: 885  LDPLLSIHIHHNLAEILPSAVDQSQSPLKDQSYSRTCEVKASKMDDSENYDNHTMKKQKV 944
                    I+++  E     +  +   L    + R   V   K+         ++K +K 
Sbjct: 812  --------IYNSKYEDHGKNLPINYFGLDHFIHLRVWYVLLHKIYFFIASAYFSLKNEKF 871

Query: 945  SETSYAPCSDTEKILDNPLKPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFT 1004
                Y    D  + + + +    S          K  E   +K++     K+   Q+ + 
Sbjct: 872  ENEFYLLAQDLRRKIMSDVIIKTS----------KHLEEFSEKFIPKKLVKIPRLQKSYA 931

Query: 1005 KSHMQVCFYVLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSS 1064
            K        ++  +  +E+ N++ + E  D  E    F   LI  ++  +    ++    
Sbjct: 932  KG-------LITGHGIIEDYNRL-YKELNDQKEVLIKFRDRLIHLMKLPLLDQESDPTGD 991

Query: 1065 RVGSRFRSINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMEN--PKEEDIERVRYCQNC 1124
                   + + + Y        ++  R ++ DR+  +  T+      E ++E+ +  ++ 
Sbjct: 992  EYEESLNAQSEISY-------CIDVYRQMLSDRVAAVSGTINTFVSHETELEKYKLIESI 1051

Query: 1125 QADSNGPPCVLCELDE----LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFY 1184
            +           E D+     F+E E    + ++  G +++ V   ++A  + +      
Sbjct: 1052 KKSEKSLDKQAEERDKKYLLYFEEREEARPKADQ-YGSLINIVSRLLDASNRST------ 1111

Query: 1185 WSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHPSELEVVL----GVIKNFCKTQLGKE 1244
             S  + +KN+      YE  +     +  +  K   EL ++       I+ + + Q   +
Sbjct: 1112 -SSFETSKNMEE----YERIDAMAKEQSRICQKLEKELSIIQLTYNSRIEYYKQLQEISD 1171

Query: 1245 SIAAAN-KQLHLLEYMRKEYGHARSL--AIAQAQVLNAHDEIKMATTRLCLRDNDDDDSA 1304
            S+       + L  Y++ +    + L   I +A V+    EI           N  +   
Sbjct: 1172 SLMPPPVSNISLNNYVKDDEKKQKFLNSVIIKASVI-LEKEISEKQDEASQTTNVAELVN 1231

Query: 1305 YAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEP 1364
              ISE  +P                     G +  L+ L + K NT    +   +  +  
Sbjct: 1232 QKISEMNIP---------------------GHIHLLRELEEEKSNTQRKIAHFESRRRYL 1291

Query: 1365 AIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKI 1424
              +   +  K+E+      + C +C++ +  Q  +  CGH+ C  CL    E  L+ S  
Sbjct: 1292 TNLYEHIVLKAES-----HQICIICRD-IIKQGFITTCGHLYCSFCL----EAWLKHSS- 1324

Query: 1425 QTQWVMCPTCRQHTDFGNIAFADDSKN------------------EILDPSTLHETSREH 1484
                  CP C+   +  N  +  +S++                  EILD     + S   
Sbjct: 1352 -----SCPMCKTKLNKNNAYYIGESRDIYSRQEFVTGFNKRDERLEILDDEAYRQISN-- 1324

Query: 1485 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1544
               + ++ S+G+KI+ + + +L++K+ +   KV+VFS W DVLDVL  +F ANGI FIR 
Sbjct: 1412 ---MELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRF 1324

Query: 1545 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1604
             G  K+ T +  F+                 E +S QVL L  +  ++GL L  A HV +
Sbjct: 1472 DGKSKN-TCLKRFK-----------------EERSLQVLTLHARSQSSGLTLTNATHVFM 1324

Query: 1605 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSR 1609
             EPLLN   E QAISRVHRIGQ   TFV+ +IV+DTVE  I  L+ ++
Sbjct: 1532 CEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVEDTVEGHILNLSLTK 1324

BLAST of MS003347 vs. ExPASy Swiss-Prot
Match: Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.9e-41
Identity = 142/525 (27.05%), Postives = 213/525 (40.57%), Query Frame = 0

Query: 287 LLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRM--FYNPFS 346
           LL +LRPYQ++A +WM Q EK   GN    E   ++ P C    CL     +  + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEK---GNC-TDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403

Query: 347 GNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSV---------FEGEKGFGN 406
           G+ T+    TL    GGILAD MGLGKT+  ++ +L+H             +EG+K   +
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISS 463

Query: 407 ESQQIVGG--QRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPK 466
               +     + T+F                                       +G+  +
Sbjct: 464 SVDDLTSPPVKATKF---------------------------------------LGFDKR 523

Query: 467 GRVLKPIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCP 526
               K +   GGN                                          LIVCP
Sbjct: 524 LLEQKSVLQNGGN------------------------------------------LIVCP 583

Query: 527 APILFQWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKED 586
             +L QW  EI  H +PGS+ + V+ G      +  LS        SD+++T+Y VL  +
Sbjct: 584 MTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSE 643

Query: 587 LSHD-SDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLY 646
            S + S  HEG                 +  + W+RI LDE   ++++ +  +  A+ L 
Sbjct: 644 FSQENSADHEG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALV 703

Query: 647 ASHRWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFF 706
           A  RWC+TGTPIQ  LEDLY LLRF++  P+  + WW ++++ P+E  D   ++      
Sbjct: 704 ADRRWCLTGTPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSIL 736

Query: 707 KQIMWRSLKIHVTDE----LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGL 766
           K IM R  K     E    L LPP + +V +   S  E  FY         + R  ++  
Sbjct: 764 KPIMLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK-- 736

Query: 767 KDDFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
            D FV++               + H  A  +L  LL+LRQ C HP
Sbjct: 824 FDQFVEQG-------------KVLHNYA-SILELLLRLRQCCDHP 736


HSP 2 Score: 107.5 bits (267), Expect = 1.5e-21
Identity = 82/311 (26.37%), Postives = 145/311 (46.62%), Query Frame = 0

Query: 1327 GSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLF 1386
            G SS L    +       +++  E   K ++  CP+C E L +  ++  C H +C +CL 
Sbjct: 761  GKSSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLL 820

Query: 1387 AMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREHETFITV 1446
            A    +           +CP CR             SK E++   T      E    + V
Sbjct: 821  ASWRNSTSG--------LCPVCRNTV----------SKQELITAPT------ESRFQVDV 880

Query: 1447 QGSY--GTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGR 1506
            + ++   +KI A++  +  ++ + SK+  ++FS W   LD+LQ   + N  +F+R+ G  
Sbjct: 881  EKNWVESSKITALLEELEGLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG-- 940

Query: 1507 KSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1566
                         ++ ++  K   +  E  S  VLL+ ++ G  G+NL  A +  +++P 
Sbjct: 941  ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1000

Query: 1567 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKNQDQP 1626
             NPA E QA+ R+HRIGQ  +  + RFIVK TVEE +  +   ++    I+G   +Q+  
Sbjct: 1001 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQR--MISGALTDQEVR 1028

Query: 1627 FLTLKEVESLF 1636
               ++E++ LF
Sbjct: 1061 SARIEELKMLF 1028

BLAST of MS003347 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-38
Identity = 144/523 (27.53%), Postives = 208/523 (39.77%), Query Frame = 0

Query: 291  LRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALE-CLDTCTRMFYNPFSGNLTL 350
            LRPYQ++A YWM + EK       V +  + + P   A   C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 351  SAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFK 410
                      GGILAD MGLGKT+  +A IL+          G GN   +          
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILA--------RPGRGNPENE---------- 735

Query: 411  RLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIK 470
                                       D+  A  +AD                       
Sbjct: 736  ---------------------------DVLVADVNAD----------------------- 795

Query: 471  KKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTR 530
             KRN+K                  E+  A  T  A   TLI+CP  +L QW  E+  H++
Sbjct: 796  -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855

Query: 531  PGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMR 590
            P ++ +LVY G   T  +  ++       + D++LT+Y VL      D            
Sbjct: 856  PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915

Query: 591  FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
                  +  +   RI W+RI LDE   ++S  T A +    L +  RWC+TGTP+Q KLE
Sbjct: 916  ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975

Query: 651  DLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 710
            DLY LL F+   P+  + WW ++I+ PYE  DP  ++      + +M R  K     E  
Sbjct: 976  DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000

Query: 711  --LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDA 770
              L+LPP + QV     S  E  FY         + R  +Q   D FV           A
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFV-----------A 1000

Query: 771  PSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 809
               +L  + +   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 QGKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000


HSP 2 Score: 106.3 bits (264), Expect = 3.4e-21
Identity = 87/322 (27.02%), Postives = 141/322 (43.79%), Query Frame = 0

Query: 1320 SKQNTPLGSSSNLALTQEPAIMSTS------MEQKSENTPKADEESCPVCQEKLSNQKMV 1379
            S+Q   L S +   L   P  +S +      +E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053

Query: 1380 FQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTL 1439
              C H +C +CL          S       +CP CR              + E++     
Sbjct: 1054 TPCAHRMCRECLLT--------SWRSPSCGLCPICRTIL----------KRTELIS---- 1113

Query: 1440 HETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 1499
              T       +       +K+  +++ +  IK + S  K +VFS W   LD+L+      
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173

Query: 1500 GITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLL 1559
            G  F+R  G              K+  K   K   +  E K   +LL+ ++ G  GLNL 
Sbjct: 1174 GFEFLRFDG--------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLT 1233

Query: 1560 EAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSF 1619
             A  V L++P  NPA E QAI R+HRIGQ+   FV RFIVKDTVEE + ++   ++    
Sbjct: 1234 AASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQR--M 1276

Query: 1620 ITGNAKNQDQPFLTLKEVESLF 1636
            I G   +++     L+E++ LF
Sbjct: 1294 IAGALTDEEVRSARLEELKMLF 1276

BLAST of MS003347 vs. ExPASy TrEMBL
Match: A0A6J1BYG6 (E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415 PE=3 SV=1)

HSP 1 Score: 3293.8 bits (8539), Expect = 0.0e+00
Identity = 1659/1675 (99.04%), Postives = 1664/1675 (99.34%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQSRPHRSGGIRLEIHANPETQVDI+VVLET+KSSDIKLNEINEPFYVEVDPTGWK
Sbjct: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDISVVLETEKSSDIKLNEINEPFYVEVDPTGWK 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI
Sbjct: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS
Sbjct: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN
Sbjct: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG
Sbjct: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVL+PIDTEGGNCIKKKRNKKNRVNV
Sbjct: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLRPIDTEGGNCIKKKRNKKNRVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
            VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG
Sbjct: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP
Sbjct: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL
Sbjct: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL
Sbjct: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA
Sbjct: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA SLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK
Sbjct: 841  GIAIIEKKFSEAFSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960
            DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN
Sbjct: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPLKPHRSSSYFN 960

Query: 961  DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLYSRLENLNKVWWLEAV 1020
            DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVC  +       E+LNKVWWLEAV
Sbjct: 961  DSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCSEL----RERESLNKVWWLEAV 1020

Query: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080
            DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV
Sbjct: 1021 DHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKYHVQTGLDLLEASRNV 1080

Query: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140
            VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV
Sbjct: 1081 VLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDELFQEYEARLFRLNKV 1140

Query: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVSKHP 1200
            QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMV KHP
Sbjct: 1141 QGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEESNKRDVGEKVMVCKHP 1200

Query: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260
            SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI
Sbjct: 1201 SELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEI 1260

Query: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320
            KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS
Sbjct: 1261 KMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLSRVKGKLRYLKGLVQS 1320

Query: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380
            KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI
Sbjct: 1321 KQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMI 1380

Query: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440
            CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH
Sbjct: 1381 CCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREH 1440

Query: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500
            ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM
Sbjct: 1441 ETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRM 1500

Query: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560
            KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL
Sbjct: 1501 KGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVL 1560

Query: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620
            VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN
Sbjct: 1561 VEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKN 1620

Query: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1676
            QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA
Sbjct: 1621 QDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAAMAAERRLREHTA 1671

BLAST of MS003347 vs. ExPASy TrEMBL
Match: A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 2851.2 bits (7390), Expect = 0.0e+00
Identity = 1439/1691 (85.10%), Postives = 1549/1691 (91.60%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   +P+TQVDIN ++ET +SSDIKL+E+NEP +VEVD TGW 
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVETVESSDIKLDEVNEPLFVEVDRTGWH 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEVVLMD+R EH FVG Q ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61   SNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS+DISLELI++ +EE  + CSVVLSG+FDGPD+AISGLVHLA+LKF+TLRPVMG + S
Sbjct: 121  LSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSFS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V STN
Sbjct: 181  QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGSTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +D+H   E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  MDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FS N GVGENVQLISPLC+ LECLDT +RMFYNPFSG+LT +AE+ LPHVYG
Sbjct: 301  WMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSHRMSVFE  KG  +E Q +VG QRT+FKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K KRNK+N VNV
Sbjct: 421  GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
             VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481  TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLS+T  VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541  VRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV   VSSD PSDILITH DAGKLL
Sbjct: 721  TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            +TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781  NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841  GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
            DQ Y RTCEVKASKMDDSE  DNH +KKQKV+ T YAPCSDTEKI+D+PL          
Sbjct: 901  DQFYPRTCEVKASKMDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAK 960

Query: 961  -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
                 +PHRSSSYFND+  RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC     L 
Sbjct: 961  KEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020

Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
            SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080

Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
            HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
            LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRLCLR NDDDDSAYAISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
             LSNQKMVFQCGHM CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
            E LDPSTLH+TSRE E  I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
            LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+  A+VPE++SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH+
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1675
            LNRSRE+SSFITGN KNQDQP  TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA 
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

BLAST of MS003347 vs. ExPASy TrEMBL
Match: A0A6J1JXA7 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489159 PE=3 SV=1)

HSP 1 Score: 2834.7 bits (7347), Expect = 0.0e+00
Identity = 1432/1692 (84.63%), Postives = 1545/1692 (91.31%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQ +PHRSGGIR E   NP+T+VD N ++ET +SSDIKL+E  EPF+VEVD TGW 
Sbjct: 1    MGRRKQKQPHRSGGIRFEDRVNPKTEVDGNGIVETVESSDIKLDEGKEPFFVEVDRTGWH 60

Query: 61   SNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWPI 120
            SN+H DISEVVLMD+R EH FVG + ++ F ESSRYSLRFRLCN N FLL RIKFGHWP+
Sbjct: 61   SNEHHDISEVVLMDIRLEHPFVGIRSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWPV 120

Query: 121  LSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATLS 180
            LSS++ISLELI++ +EE  + CSVVLSG+FDGPD+AISGLVHLANLKF+TLRPVMG + S
Sbjct: 121  LSSNEISLELIERRIEEDMNGCSVVLSGSFDGPDEAISGLVHLANLKFVTLRPVMGVSFS 180

Query: 181  RSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKSTN 240
            ++ GSLRLRVE+LSSA DAC SI D+GRQLWKKSMM+TIAWLRPEV SSE KYG+V+STN
Sbjct: 181  QNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVESTN 240

Query: 241  VDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300
            +++H   E GD +S++RKH+NFD+V FYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY
Sbjct: 241  MEAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAAY 300

Query: 301  WMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVYG 360
            WMVQREK FS N GVGEN QLISPLC+ LECLDT  RMFYNPFSG+LT +AE+TLPHVYG
Sbjct: 301  WMVQREKGFSRNFGVGENGQLISPLCMPLECLDTRLRMFYNPFSGDLTWNAEQTLPHVYG 360

Query: 361  GILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECVC 420
            GILADEMGLGKTIELLACILSH MSVF+G KGF +E QQ+V  QRT+FKRLKRERVECVC
Sbjct: 361  GILADEMGLGKTIELLACILSHPMSVFKGGKGFEDEMQQLVEDQRTKFKRLKRERVECVC 420

Query: 421  GAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVNV 480
            GAVSENHRY+GLWVQCDICDAWQHADCVGYSPKGR+L+P+DT+ GNC K K NK+N VNV
Sbjct: 421  GAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKTNKRNTVNV 480

Query: 481  VVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYEG 540
             VRD +HVC+PCLELMQATD+PM T ATLIVCPAPILFQWHAEILRHT PGSIKLLVYEG
Sbjct: 481  TVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYEG 540

Query: 541  VRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTP 600
            VRDTSLS+ L VQIND++NSDI+LTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PTP
Sbjct: 541  VRDTSLSNPLPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPTP 600

Query: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660
            LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA
Sbjct: 601  LTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKA 660

Query: 661  SPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWL 720
            SPF+V+RWWVEVIRDPYERRDPG MEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTWL
Sbjct: 661  SPFNVHRWWVEVIRDPYERRDPGTMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTWL 720

Query: 721  TFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKLL 780
            TFSPIEE+FYQRQHETCVSYAREVIQGLKDDFVKRKV   VSSDAPSDILITH DAGKLL
Sbjct: 721  TFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDAPSDILITHADAGKLL 780

Query: 781  STLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALA 840
            +TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR+VVALNALA
Sbjct: 781  NTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNALA 840

Query: 841  GIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPLK 900
            GIAIIEKKFSEA+SLYKEALELAE NNEDFR+DPLLSIHIHHNLAEILP A+DQ QSPLK
Sbjct: 841  GIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPLK 900

Query: 901  DQSYSRTCEVKASKMDDSENYDNHTMKKQKVSETSYAPCSDTEKILDNPL---------- 960
            DQ Y RTCEVKAS+MDDSE  DNH +KKQKV+ T Y PCSDTEKI+D+PL          
Sbjct: 901  DQFYPRTCEVKASRMDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAK 960

Query: 961  -----KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFYVLLLY 1020
                 +PHRSS+YFND+  RKACE MRQKYLAVFSSKL IAQQEFTKS++QVC     L 
Sbjct: 961  KEENYEPHRSSNYFNDNYLRKACEDMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE---LK 1020

Query: 1021 SRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSINALKY 1080
            SR ENLN+VWWL+AV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI+ALKY
Sbjct: 1021 SR-ENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSIDALKY 1080

Query: 1081 HVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVLCELDE 1140
            HVQTGLDLLEASR +V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSNGPPCVLCELD+
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDQ 1140

Query: 1141 LFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRVGYEES 1200
            LFQEYEARLFRLN VQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSR GYEES
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1261 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLSLAMLS 1320
            LAIAQAQVLNAHDEIKMATTRLCLR ND DDSAYAISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDADDSAYAISEHELPAASVQYSSDKFVSLAMLS 1320

Query: 1321 RVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQE 1380
            RVKGKLRYLKGLVQSKQN PLGSSSN A TQ P I STS+EQKSENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1381 KLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKN 1440
             LSNQKMVFQCGH+ CCKCLFAMTEK L+DS IQT+WVMCPTCRQHTDFGNIA+ADDSKN
Sbjct: 1381 TLSNQKMVFQCGHITCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1441 EILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500
              LDPSTLH+TSRE E  I VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ATLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1501 LQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQVLLLLI 1560
            LQYAFAAN ITF+RMKGGRKS TAISEFRGQKINAKENHKK+  A+VPEL+SAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPELRSAQVLLLLI 1560

Query: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHK 1620
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIH 
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHS 1620

Query: 1621 LNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAPSMAAA 1676
            LNRSRE+SSFITGN KNQDQP  TLKEVESLFAS+ SPL +SD+KESETLRHLAPSMAA 
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

BLAST of MS003347 vs. ExPASy TrEMBL
Match: A0A1S3C3N1 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 2823.5 bits (7318), Expect = 0.0e+00
Identity = 1437/1696 (84.73%), Postives = 1544/1696 (91.04%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLET-DKSSDIKLNEINEPFYVEVDPTGW 60
            MGRRKQ +PH SGGIR E   +P+TQ+DIN V ET  ++SDIKL+E+NEP +VEVD +GW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 61   KSNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWP 120
             SN+H DISEV L+D+R E+ FV F  D S  E+SRYSLRFRLCNVN  LL RIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 121  ILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATL 180
            +LSS+DI LELI++ +EE   ACSVVLSGNFDGPD+AISGLVHLANLK MTLRPV G + 
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 181  SRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKST 240
            S++TGSLRLRVE+LSSA DACESIFDNGRQLWKKSMM+TIAWLRPEV  SE KYG+VKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 241  NVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
            N+D+H  HE GD +SN+RKH+NFDT+ FYDAIKPSKD+P LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 301  YWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVY 360
            YWMVQREK FSGNSG+GENVQLISPLC+AL+CL+TC R+FYNPFSGNLTL+AE+ LPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 361  GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 420
            GGILADEMGLGKTIELLACILSH+MSVFEG KGF  E +Q+V  QRTEFKRLKRERVEC+
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 421  CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 480
            CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGR++KP+DTE GN  ++KRN KN +N
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 481  VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYE 540
            V+VR  +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHTRPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 541  GVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSL  TLSVQINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 661  ASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPF+V++WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 721  LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKL 780
            L FSP+EEHFYQRQHETCVSYAREVIQGLKDDFVKRKV DCVSSD PSD+L+TH DAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 841  AGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPL 900
            AGIAII KKF EA SLYKEALELAE N EDFRLDPLLSIHIHHNLAEILP AV+QSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 901  KDQSYSRTCEVKASKMDDSENYDNHT--MKKQKVSETSYAPCSD--TEKILDNPL----- 960
            KDQ   RTCEVKAS+MDDSE Y  H   MKKQKVSET YA CS+  T K++D+PL     
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 961  ----------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFY 1020
                      +PHRS+SYF++   RK CE MRQKYLAVFSSKLSIAQQEFTKS+MQV   
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1021 VLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSI 1080
            +       ENLN VWWLEAV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI
Sbjct: 1021 L----KDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSI 1080

Query: 1081 NALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVL 1140
            +ALKYHVQ+GLDLLEASR VVLDRLLEIDQTMENPKEEDIERVRYC+NCQADSNGPPCVL
Sbjct: 1081 SALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVL 1140

Query: 1141 CELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRV 1200
            CELDELFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQ+KN SSS+V
Sbjct: 1141 CELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKV 1200

Query: 1201 GYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEY 1260
            G+EE NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESIAAANKQLHLLEYMRKEY
Sbjct: 1201 GHEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEY 1260

Query: 1261 GHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQYSSDKFLS 1320
            GHARSLAIAQA VLNAHDEIKMATTRLCLRDNDDD SA++ISEHELPAASVQYSSDKF+S
Sbjct: 1261 GHARSLAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMS 1320

Query: 1321 LAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEESC 1380
            LAMLSRVKGKLRYLKGLVQSKQ  PL SSS+LALTQEPAI ST+MEQK+ENT KADEESC
Sbjct: 1321 LAMLSRVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESC 1380

Query: 1381 PVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFA 1440
            P+CQEKLSNQKMVFQCGH+ CCKCLFAMTEKTL  SKIQT+WVMCPTCRQHTDFGNIA+A
Sbjct: 1381 PICQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYA 1440

Query: 1441 DDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWN 1500
            DDSKNE LDPSTL ETSR+HE  ITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWN
Sbjct: 1441 DDSKNETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWN 1500

Query: 1501 DVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQV 1560
            DVLDVL YAFAAN I+FIRMKGGRKSQTAISEFRGQKINAKEN KKR    +PEL+SAQV
Sbjct: 1501 DVLDVLHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQV 1560

Query: 1561 LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE 1620
            LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE
Sbjct: 1561 LLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVE 1620

Query: 1621 ESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLAP 1675
            ESI+KLNRSRE+SSFITGNAKNQDQPFLTLKEVESLFASKASPLG++DDK SETLR LAP
Sbjct: 1621 ESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAP 1680

BLAST of MS003347 vs. ExPASy TrEMBL
Match: A0A0A0K8S1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 PE=3 SV=1)

HSP 1 Score: 2812.3 bits (7289), Expect = 0.0e+00
Identity = 1433/1697 (84.44%), Postives = 1534/1697 (90.39%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETD-KSSDIKLNEINEPFYVEVDPTGW 60
            MGRRKQ +P+RSGGIR E   + +TQ+DIN V+ET  +SSDIKL+ +NEP +VEVD +GW
Sbjct: 101  MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 160

Query: 61   KSNKHLDISEVVLMDLRFEHHFVGFQFDDSFYESSRYSLRFRLCNVNEFLLGRIKFGHWP 120
             SN+H DISEV L D+R EH FVGF  D S  E+SRYSLRFRLCNVN  LL RIKFGHWP
Sbjct: 161  YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 220

Query: 121  ILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPVMGATL 180
            +LSS+D  LE I++ MEE   ACSVVLSGN DGPD+AISGLVHLANLK MTLRPV G   
Sbjct: 221  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 280

Query: 181  SRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYGLVKST 240
            S++ GSLRLRVE+LSSA DACESIFDNGRQLWKKSMM+TI WLRPEV  SE KYG+VKS+
Sbjct: 281  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 340

Query: 241  NVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300
            N+D+H  HE GD +SN+RKH NFDT+GFYDAIKPSKD+PMLDEDIPNLLPKLRPYQRRAA
Sbjct: 341  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 400

Query: 301  YWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKTLPHVY 360
            YWM+QREK FSGNSG+GENVQLISPLC+AL+CLDTC R+FYNPFSGNLTL+AE+ LPHVY
Sbjct: 401  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 460

Query: 361  GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 420
            GGILADEMGLGKTIELLACILSH+MSVFEG KGF  E QQ+V  QRTEFKRLKRER+EC+
Sbjct: 461  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 520

Query: 421  CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 480
            CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGR++K +DTE GN  ++KRN +N +N
Sbjct: 521  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 580

Query: 481  VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPGSIKLLVYE 540
            V+VR  +HVC PCLELMQATD+PMAT ATLIVCPAPILFQW AEILRHT PGS+KLLVYE
Sbjct: 581  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 640

Query: 541  GVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600
            GVRDTSL  TLS QINDL+NSDI+LTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT
Sbjct: 641  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 700

Query: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK
Sbjct: 701  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 760

Query: 661  ASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720
            ASPF+V+RWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 761  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 820

Query: 721  LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILITHTDAGKL 780
            L FSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKV DCV+SD PSD+LITH DAGKL
Sbjct: 821  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 880

Query: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL
Sbjct: 881  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 940

Query: 841  AGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSAVDQSQSPL 900
            AGIAIIEKKFSEA SLYKEALE AE NNEDFRLDPLLSIHIHHNLAEILP AV+QSQSPL
Sbjct: 941  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 1000

Query: 901  KDQSYSRTCEVKASKMDDSENYDNHT--MKKQKVSETSYAPCSD--TEKILDNPL----- 960
            KDQ   RTCEVKAS+MDDSE YD+H   MKKQKVSET YA CS+  T K++D+PL     
Sbjct: 1001 KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 1060

Query: 961  ----------KPHRSSSYFNDSSFRKACETMRQKYLAVFSSKLSIAQQEFTKSHMQVCFY 1020
                      +PHRSS YF++ S RK CE MRQKYLAVFSSKLSIAQQEFTKS+MQV   
Sbjct: 1061 DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1120

Query: 1021 VLLLYSRLENLNKVWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVGSRFRSI 1080
            +       EN N VWWLEAV HAEQNKDF HELIRKIEEAVSGN+NNS+ SRVGSRFRSI
Sbjct: 1121 L----KDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSK-SRVGSRFRSI 1180

Query: 1081 NALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNGPPCVL 1140
            +ALKYHVQ+GLDLLEASR VVLDRLLEIDQTM++PKEEDIERVRYC+ CQADSNGPPCVL
Sbjct: 1181 SALKYHVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVL 1240

Query: 1141 CELDELFQEYEARLFRLNKVQGGMVSSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRV 1200
            CELDELFQEYEARLFRLNKVQGGMV+SVEEAVEAQKKKSALNRFYWSLLQQNKN SSS+V
Sbjct: 1241 CELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKV 1300

Query: 1201 GYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEY 1260
            G+EE NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLE+MRKEY
Sbjct: 1301 GHEEPNKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEY 1360

Query: 1261 GHARSLAIAQAQVLNAHDEIKMATTRLCLR-DNDDDDSAYAISEHELPAASVQYSSDKFL 1320
            GHARSLAIAQAQVLNAHDEIKMATTRL LR D+DDD SA+ ISEHELPAASVQYSSDKF+
Sbjct: 1361 GHARSLAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFM 1420

Query: 1321 SLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENTPKADEES 1380
            SLAMLSRVKGKLRYLKGLVQSKQ  PL SSS+LALTQEPAI ST+ EQK+ENT KADEES
Sbjct: 1421 SLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEES 1480

Query: 1381 CPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAF 1440
            CPVCQEKL+NQKMVFQCGH+ CCKCLFAMTEKTL  SKIQT+WVMCPTCRQHTDFGNIA+
Sbjct: 1481 CPVCQEKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAY 1540

Query: 1441 ADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSW 1500
            ADDSKNE LDPST  ETSREHE  ITVQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSW
Sbjct: 1541 ADDSKNETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSW 1600

Query: 1501 NDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKR--AQVPELKSAQ 1560
            NDVLDVL YAF AN ITFIRMKGGRKSQTAISEFRGQKINAKEN KKR    +PEL+SAQ
Sbjct: 1601 NDVLDVLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQ 1660

Query: 1561 VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV 1620
            VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV
Sbjct: 1661 VLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTV 1720

Query: 1621 EESIHKLNRSRENSSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDDKESETLRHLA 1675
            EESI+KLNRSRE+SSFITGNAKNQDQPFLTLKEVESLFASKASPLG++DDK SETLR LA
Sbjct: 1721 EESIYKLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLA 1780

BLAST of MS003347 vs. TAIR 10
Match: AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )

HSP 1 Score: 1761.9 bits (4562), Expect = 0.0e+00
Identity = 969/1708 (56.73%), Postives = 1205/1708 (70.55%), Query Frame = 0

Query: 1    MGRRKQSRPHRSGGIRLEIHANPETQVDINVVLETDKSSDIKLNEINEPFYVEVDPTGWK 60
            MGRRKQS+P R+ G  L      E+  +     E + S D  + +I++P+YV +  +   
Sbjct: 1    MGRRKQSKPQRAVG--LITPTGSESDRNQLPGDEAEGSGDKNVEDIDKPYYVNICLSSRV 60

Query: 61   S--NKHLDISEVVLMDLRFEHHFVG----FQFDDSFYESSRYSLRFRLCNVNEFLLGRIK 120
            S   ++ D++EVVL +       VG    F            SLRFRLCNV  F + RIK
Sbjct: 61   SEQQQNFDLAEVVLTNFSVRER-VGSSSTFTKPIEVDHDLDCSLRFRLCNVTNF-VDRIK 120

Query: 121  FGHWPILSSSDISLELIDKGMEEGTDACSVVLSGNFDGPDDAISGLVHLANLKFMTLRPV 180
             GHWP+LSSSDI+LEL+D  + +  +  SV+ S +FDGP + +SGL HLA++KF+TLR +
Sbjct: 121  LGHWPVLSSSDITLELVDNKVSD-DEVGSVIWSASFDGPGEGVSGLAHLASIKFLTLRLM 180

Query: 181  MGATLSRSTGSLRLRVEMLSSALDACESIFDNGRQLWKKSMMSTIAWLRPEVESSEAKYG 240
             G   +    S R+RVEML  A DAC+S+ +N RQ+WKKSM+  ++WLRPEV +SEA+YG
Sbjct: 181  PG---NEGLLSPRVRVEMLQQAFDACDSLLENTRQIWKKSMIHVMSWLRPEVMTSEARYG 240

Query: 241  LVKSTNVDSHSLHEMGDGSSNTRKHINFDTVGFYDAIKPSKDDPMLDEDIPNLLPKLRPY 300
              +    D  S     D + ++ K  +FD   FY+AIKPSK D ML +DI +LLP+LRPY
Sbjct: 241  -TRFNVKDIESSVVTEDETLDSSKQSSFDAAAFYEAIKPSKTDAMLGDDIADLLPELRPY 300

Query: 301  QRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRMFYNPFSGNLTLSAEKT 360
            QRRAAYWMVQRE+      G  E+ Q ISPL I++  LD+ T+MF NPFSGN++L+ E  
Sbjct: 301  QRRAAYWMVQRERGDPITLGDKEDNQFISPLSISVGFLDSATKMFLNPFSGNISLTPEYF 360

Query: 361  LPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRE 420
             P + GGILADEMGLGKT+ELLACI SHR    E E    N S  +        +RLKRE
Sbjct: 361  SPRIQGGILADEMGLGKTVELLACIFSHRKPA-EDEISVSNGS-SVTDVLNAGLRRLKRE 420

Query: 421  RVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKP---IDTEGGNCIKKK 480
            RVEC+CGAVSE+H+YKG+WVQCD+CDAWQHADCVGYSPKG+  K    ID +      +K
Sbjct: 421  RVECICGAVSESHKYKGVWVQCDLCDAWQHADCVGYSPKGKGKKDSQHIDEKA----SQK 480

Query: 481  RNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTRPG 540
            ++KK+   ++VR+G+++C  C EL+Q T +P++T ATLIVCPAPIL QWH+EI RHTR G
Sbjct: 481  KSKKDATEIIVREGEYICQMCSELLQVTASPISTGATLIVCPAPILPQWHSEITRHTRLG 540

Query: 541  SIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMRFQ 600
            S+   +YEGVR+ SLS    + I +L+N+DI+LT+YDVLKEDL+HD DRH+GDR  +RFQ
Sbjct: 541  SLITCIYEGVRNASLSEEPMIDITELLNADIVLTTYDVLKEDLTHDFDRHDGDRHCLRFQ 600

Query: 601  KRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDL 660
            KRYPVIPTPLTRIFWWRICLDE QMVESNA AATEMA RLY  HRWCITGTPIQRKL+DL
Sbjct: 601  KRYPVIPTPLTRIFWWRICLDEAQMVESNAAAATEMALRLYTKHRWCITGTPIQRKLDDL 660

Query: 661  YGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLP 720
            +GLL+F+KA+PFDV RWW+EVIRDPYERRD  AMEFTHKFFKQ+MWRS K+HV DELQLP
Sbjct: 661  FGLLKFLKANPFDVSRWWIEVIRDPYERRDTKAMEFTHKFFKQVMWRSSKVHVADELQLP 720

Query: 721  PQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDAPSDILI 780
            PQEE V+WL FS IEEHFY RQH+TCVSYAREVI+ LK D +KR      SSD P   L+
Sbjct: 721  PQEECVSWLKFSAIEEHFYSRQHDTCVSYAREVIETLKRDILKR---GHTSSDNP---LV 780

Query: 781  THTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRR 840
            TH +A KLL++LLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLV KT+ EGEEALR 
Sbjct: 781  THAEAAKLLNSLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVKKTQSEGEEALRV 840

Query: 841  SVVALNALAGIAIIEKKFSEAVSLYKEALELAEGNNEDFRLDPLLSIHIHHNLAEILPSA 900
             +VALN +A IA+++++FSEAVSLYKEAL + E + EDFRLDPLL+IHI HNLAEILP A
Sbjct: 841  LIVALNGIAAIAMLKQEFSEAVSLYKEALSITEEHAEDFRLDPLLNIHILHNLAEILPMA 900

Query: 901  VDQSQSPLKDQSYSRTCEVKASKMDDSENYDNH-TMKKQKVSETSYAPCSDTEKILDNPL 960
                +S     S S   E K    DD    D+H   K+Q+++E        +E +     
Sbjct: 901  ----KSYGGKLSASGRPETKIDVKDD----DHHRASKRQRINELESLTHDSSETV----- 960

Query: 961  KPHRSSSYFNDSSFRK-------------ACETMRQKYLAVFSSKLSIAQQEFTKSHMQV 1020
              H+  +   D+  +K              C+T++ KYL+ F+SKLS AQ EF KS+ QV
Sbjct: 961  --HQREAIAPDNGLKKDGECHEECKTLDIVCDTLKVKYLSAFNSKLSAAQHEFKKSYNQV 1020

Query: 1021 CFYVLLLYSRLENLNK---VWWLEAVDHAEQNKDFYHELIRKIEEAVSGNINNSRSSRVG 1080
                      L N+ K   VWWL+A+   EQNKDF  EL RKIEEA+ GN+NNS SSR  
Sbjct: 1021 S-------ESLSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSSRES 1080

Query: 1081 SRFRSINALKYHVQTGLDLLEASRNVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSN 1140
            SRFR+I+ +K H+QT +D+LE SR  V+DR+LEIDQTME PK EDIER+  C+ C  +S+
Sbjct: 1081 SRFRTIHGMKLHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNKNSD 1140

Query: 1141 GPPCVLCELDELFQEYEARLFRLNKVQGGM--VSSVEEAVEAQKKKSALNRFYWSLLQQN 1200
            GPPC+ CELDELFQEYEARLFRLNK + G+  +++ EE V  QKK+ A N F + L  ++
Sbjct: 1141 GPPCIHCELDELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLSSRS 1200

Query: 1201 KNLSSSRVGYEESNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHL 1260
            K+L++SR   EE  KR+ G+ V++SK PSE E+VLGVI+N CKT L +ES  AA K LH 
Sbjct: 1201 KDLNASRGDDEEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKHLHT 1260

Query: 1261 LEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDDSAYAISEHELPAASVQ 1320
            LE MRKEY HAR LA  QAQ+L A+DEI M+T RL LR+++DD S YA+   EL  ASV 
Sbjct: 1261 LEVMRKEYVHARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVASVL 1320

Query: 1321 YSSDKFLSLAMLSRVKGKLRYLKGLVQSKQNTPLGSSSNLALTQEPAIMSTSMEQKSENT 1380
             ++DKF++ + L  +KGKLRYLKGL++SKQ     S    +   E    S   EQ+SEN 
Sbjct: 1321 NTNDKFMAQSSLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQESENL 1380

Query: 1381 PKADEESCPVCQEKLSNQKMVFQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHT 1440
             K D E+CP+C E L NQKMVFQCGH  CC C FAMTE+      +Q +WVMCP CRQHT
Sbjct: 1381 LKRD-EACPICHEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQ-KWVMCPICRQHT 1440

Query: 1441 DFGNIAFADDSKNEILDPSTLHETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAK 1500
            D  NIA+ADD +N     S+  +  ++ E  + VQGSYGTKIEAV RRILWIK +D + K
Sbjct: 1441 DVRNIAYADDRRNS----SSSDQDHKDSEASLVVQGSYGTKIEAVTRRILWIKSSDPQTK 1500

Query: 1501 VLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPE 1560
            VLVFSSWNDVLDVL++AFAAN IT IRMKGGRKSQTAIS+F+G +   KE  K  +   E
Sbjct: 1501 VLVFSSWNDVLDVLEHAFAANSITCIRMKGGRKSQTAISKFKGSE---KETQKTNSHQKE 1560

Query: 1561 LKSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFI 1620
             KS QVLLLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVHRIGQE  T VHRF+
Sbjct: 1561 EKSIQVLLLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTLVHRFL 1620

Query: 1621 VKDTVEESIHKLNRSREN--SSFITGNAKNQDQPFLTLKEVESLFASKASPLGDSDD--- 1676
            V  TVEESI+KLNR++    SSF + N KNQDQ FLTLK++ESLFAS  +   + +    
Sbjct: 1621 VSGTVEESIYKLNRNKNTNLSSFSSRNTKNQDQQFLTLKDLESLFASPTAETAEMEQNPG 1656

BLAST of MS003347 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 172.6 bits (436), Expect = 2.8e-42
Identity = 142/525 (27.05%), Postives = 213/525 (40.57%), Query Frame = 0

Query: 287 LLPKLRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALECLDTCTRM--FYNPFS 346
           LL +LRPYQ++A +WM Q EK   GN    E   ++ P C    CL     +  + N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEK---GNC-TDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403

Query: 347 GNLTLSAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSV---------FEGEKGFGN 406
           G+ T+    TL    GGILAD MGLGKT+  ++ +L+H             +EG+K   +
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYEGDKVISS 463

Query: 407 ESQQIVGG--QRTEFKRLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPK 466
               +     + T+F                                       +G+  +
Sbjct: 464 SVDDLTSPPVKATKF---------------------------------------LGFDKR 523

Query: 467 GRVLKPIDTEGGNCIKKKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCP 526
               K +   GGN                                          LIVCP
Sbjct: 524 LLEQKSVLQNGGN------------------------------------------LIVCP 583

Query: 527 APILFQWHAEILRHTRPGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKED 586
             +L QW  EI  H +PGS+ + V+ G      +  LS        SD+++T+Y VL  +
Sbjct: 584 MTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLS-------QSDVVITTYGVLTSE 643

Query: 587 LSHD-SDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLY 646
            S + S  HEG                 +  + W+RI LDE   ++++ +  +  A+ L 
Sbjct: 644 FSQENSADHEG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALV 703

Query: 647 ASHRWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFF 706
           A  RWC+TGTPIQ  LEDLY LLRF++  P+  + WW ++++ P+E  D   ++      
Sbjct: 704 ADRRWCLTGTPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSIL 736

Query: 707 KQIMWRSLKIHVTDE----LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGL 766
           K IM R  K     E    L LPP + +V +   S  E  FY         + R  ++  
Sbjct: 764 KPIMLRRTKSSTDREGRPILVLPPADARVIYCELSESERDFYD------ALFKRSKVK-- 736

Query: 767 KDDFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
            D FV++               + H  A  +L  LL+LRQ C HP
Sbjct: 824 FDQFVEQG-------------KVLHNYA-SILELLLRLRQCCDHP 736


HSP 2 Score: 107.5 bits (267), Expect = 1.1e-22
Identity = 82/311 (26.37%), Postives = 145/311 (46.62%), Query Frame = 0

Query: 1327 GSSSNLALTQEPAIMSTSMEQKSENTPKADEESCPVCQEKLSNQKMVFQCGHMICCKCLF 1386
            G SS L    +       +++  E   K ++  CP+C E L +  ++  C H +C +CL 
Sbjct: 761  GKSSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLL 820

Query: 1387 AMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTLHETSREHETFITV 1446
            A    +           +CP CR             SK E++   T      E    + V
Sbjct: 821  ASWRNSTSG--------LCPVCRNTV----------SKQELITAPT------ESRFQVDV 880

Query: 1447 QGSY--GTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANGITFIRMKGGR 1506
            + ++   +KI A++  +  ++ + SK+  ++FS W   LD+LQ   + N  +F+R+ G  
Sbjct: 881  EKNWVESSKITALLEELEGLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG-- 940

Query: 1507 KSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1566
                         ++ ++  K   +  E  S  VLL+ ++ G  G+NL  A +  +++P 
Sbjct: 941  ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1000

Query: 1567 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSFITGNAKNQDQP 1626
             NPA E QA+ R+HRIGQ  +  + RFIVK TVEE +  +   ++    I+G   +Q+  
Sbjct: 1001 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQR--MISGALTDQEVR 1028

Query: 1627 FLTLKEVESLF 1636
               ++E++ LF
Sbjct: 1061 SARIEELKMLF 1028

BLAST of MS003347 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-39
Identity = 144/523 (27.53%), Postives = 208/523 (39.77%), Query Frame = 0

Query: 291  LRPYQRRAAYWMVQREKRFSGNSGVGENVQLISPLCIALE-CLDTCTRMFYNPFSGNLTL 350
            LRPYQ++A YWM + EK       V +  + + P   A   C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 351  SAEKTLPHVYGGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFK 410
                      GGILAD MGLGKT+  +A IL+          G GN   +          
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILA--------RPGRGNPENE---------- 735

Query: 411  RLKRERVECVCGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIK 470
                                       D+  A  +AD                       
Sbjct: 736  ---------------------------DVLVADVNAD----------------------- 795

Query: 471  KKRNKKNRVNVVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRHTR 530
             KRN+K                  E+  A  T  A   TLI+CP  +L QW  E+  H++
Sbjct: 796  -KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSK 855

Query: 531  PGSIKLLVYEGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDRHEGDRRFMR 590
            P ++ +LVY G   T  +  ++       + D++LT+Y VL      D            
Sbjct: 856  PDTVSVLVYYGGDRTHDAKAIA-------SHDVVLTTYGVLTSAYKQD------------ 915

Query: 591  FQKRYPVIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLE 650
                  +  +   RI W+RI LDE   ++S  T A +    L +  RWC+TGTP+Q KLE
Sbjct: 916  ------MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLE 975

Query: 651  DLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE-- 710
            DLY LL F+   P+  + WW ++I+ PYE  DP  ++      + +M R  K     E  
Sbjct: 976  DLYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGS 1000

Query: 711  --LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVADCVSSDA 770
              L+LPP + QV     S  E  FY         + R  +Q   D FV           A
Sbjct: 1036 LILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFV-----------A 1000

Query: 771  PSDILITHTDAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 809
               +L  + +   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 QGKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000


HSP 2 Score: 106.3 bits (264), Expect = 2.4e-22
Identity = 87/322 (27.02%), Postives = 141/322 (43.79%), Query Frame = 0

Query: 1320 SKQNTPLGSSSNLALTQEPAIMSTS------MEQKSENTPKADEESCPVCQEKLSNQKMV 1379
            S+Q   L S +   L   P  +S +      +E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053

Query: 1380 FQCGHMICCKCLFAMTEKTLQDSKIQTQWVMCPTCRQHTDFGNIAFADDSKNEILDPSTL 1439
              C H +C +CL          S       +CP CR              + E++     
Sbjct: 1054 TPCAHRMCRECLLT--------SWRSPSCGLCPICRTIL----------KRTELIS---- 1113

Query: 1440 HETSREHETFITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 1499
              T       +       +K+  +++ +  IK + S  K +VFS W   LD+L+      
Sbjct: 1114 CPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLRRR 1173

Query: 1500 GITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVPELKSAQVLLLLIQHGANGLNLL 1559
            G  F+R  G              K+  K   K   +  E K   +LL+ ++ G  GLNL 
Sbjct: 1174 GFEFLRFDG--------------KLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLT 1233

Query: 1560 EAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIHKLNRSRENSSF 1619
             A  V L++P  NPA E QAI R+HRIGQ+   FV RFIVKDTVEE + ++   ++    
Sbjct: 1234 AASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQR--M 1276

Query: 1620 ITGNAKNQDQPFLTLKEVESLF 1636
            I G   +++     L+E++ LF
Sbjct: 1294 IAGALTDEEVRSARLEELKMLF 1276

BLAST of MS003347 vs. TAIR 10
Match: AT1G61140.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 114/463 (24.62%), Postives = 182/463 (39.31%), Query Frame = 0

Query: 360 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 419
           GGILAD+ GLGKT+  +A IL  R             +Q      + E   L+ E  EC 
Sbjct: 579 GGILADDQGLGKTVSTIALILKER----------SKPAQACEESTKKEIFDLESETGECA 638

Query: 420 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 479
               S   ++            ++H+         ++L   +  GG+ + K         
Sbjct: 639 PLKPSGRSKH------------FEHS---------QLLSNENKVGGDSVGK--------- 698

Query: 480 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRH-TRPGSIKLLVY 539
                              T  P A   TL+VCP  ++ QW  E+ +  T   ++ +LVY
Sbjct: 699 ------------------VTGRPAA--GTLVVCPTSVMRQWADELHKKVTSEANLSVLVY 758

Query: 540 EGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDR-----HEGDRRFMRF--- 599
            G   T     L+    D+V +   + S +V K+ L  D D      H+G      F   
Sbjct: 759 HGSSRTKDPHELAKY--DVVVTTFSIVSMEVPKQPLVDDEDEEKDGVHDGGTAATGFCSN 818

Query: 600 -QKRYP--------------VIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASH 659
            +++YP               +  PL ++ W+R+ LDE Q +++  T        L A  
Sbjct: 819 KKRKYPPDSKKKGSKKKKVEFLSGPLAKVSWFRVVLDEAQSIKNYKTQVARACWGLRAKR 878

Query: 660 RWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQI 719
           RWC++GTPIQ  ++DLY   RF+K  P+  Y  +   I++P  R      +      K +
Sbjct: 879 RWCLSGTPIQNSIDDLYSYFRFLKYDPYSSYVLFCSTIKNPITRNPVKGYQKLQAILKTV 938

Query: 720 MWRSLKIHVTDE---LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQG--LKD 779
           M R  K  + D    + LPP+  ++  + F+  E  FY +      +  RE  +   +K 
Sbjct: 939 MLRRTKGSLLDGKPIISLPPKSIELRKVDFTVEERDFYSKLEAESRTQFREYAEAGTVKQ 955

Query: 780 DFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
           ++V                         +L  LL+LRQAC HP
Sbjct: 999 NYV------------------------NILLMLLRLRQACDHP 955


HSP 2 Score: 72.8 bits (177), Expect = 3.0e-12
Identity = 49/166 (29.52%), Postives = 82/166 (49.40%), Query Frame = 0

Query: 1472 KVLVFSSWNDVLDVLQYAFAANGITFIRMKGGRKSQTAISEFRGQKINAKENHKKRAQVP 1531
            K +VFS W  +L++L+ +  ++ I + R+ G               ++     K      
Sbjct: 1125 KAIVFSQWTKMLNLLEASLVSSHIQYRRLDG--------------TMSVAARDKAVQDFN 1184

Query: 1532 ELKSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRF 1591
             L    V+++ ++  + GLN++ A HV++++   NP  E QAI R HRIGQ     V RF
Sbjct: 1185 TLPEVTVMIMSLKAASLGLNMVAACHVLMLDLWWNPTTEDQAIDRAHRIGQTRPVTVVRF 1244

Query: 1592 IVKDTVEESIHKLNRSREN--SSFITGNAKNQDQPFLTLKEVESLF 1636
             VKDTVE+ I  L + +    +S    + K   Q  LT++++  LF
Sbjct: 1245 TVKDTVEDRILALQQKKRMMVASAFGEDEKGSRQSHLTVEDLSYLF 1276

BLAST of MS003347 vs. TAIR 10
Match: AT1G61140.2 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-27
Identity = 114/463 (24.62%), Postives = 182/463 (39.31%), Query Frame = 0

Query: 360 GGILADEMGLGKTIELLACILSHRMSVFEGEKGFGNESQQIVGGQRTEFKRLKRERVECV 419
           GGILAD+ GLGKT+  +A IL  R             +Q      + E   L+ E  EC 
Sbjct: 586 GGILADDQGLGKTVSTIALILKER----------SKPAQACEESTKKEIFDLESETGECA 645

Query: 420 CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRVLKPIDTEGGNCIKKKRNKKNRVN 479
               S   ++            ++H+         ++L   +  GG+ + K         
Sbjct: 646 PLKPSGRSKH------------FEHS---------QLLSNENKVGGDSVGK--------- 705

Query: 480 VVVRDGQHVCLPCLELMQATDTPMATVATLIVCPAPILFQWHAEILRH-TRPGSIKLLVY 539
                              T  P A   TL+VCP  ++ QW  E+ +  T   ++ +LVY
Sbjct: 706 ------------------VTGRPAA--GTLVVCPTSVMRQWADELHKKVTSEANLSVLVY 765

Query: 540 EGVRDTSLSSTLSVQINDLVNSDIILTSYDVLKEDLSHDSDR-----HEGDRRFMRF--- 599
            G   T     L+    D+V +   + S +V K+ L  D D      H+G      F   
Sbjct: 766 HGSSRTKDPHELAKY--DVVVTTFSIVSMEVPKQPLVDDEDEEKDGVHDGGTAATGFCSN 825

Query: 600 -QKRYP--------------VIPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASH 659
            +++YP               +  PL ++ W+R+ LDE Q +++  T        L A  
Sbjct: 826 KKRKYPPDSKKKGSKKKKVEFLSGPLAKVSWFRVVLDEAQSIKNYKTQVARACWGLRAKR 885

Query: 660 RWCITGTPIQRKLEDLYGLLRFVKASPFDVYRWWVEVIRDPYERRDPGAMEFTHKFFKQI 719
           RWC++GTPIQ  ++DLY   RF+K  P+  Y  +   I++P  R      +      K +
Sbjct: 886 RWCLSGTPIQNSIDDLYSYFRFLKYDPYSSYVLFCSTIKNPITRNPVKGYQKLQAILKTV 945

Query: 720 MWRSLKIHVTDE---LQLPPQEEQVTWLTFSPIEEHFYQRQHETCVSYAREVIQG--LKD 779
           M R  K  + D    + LPP+  ++  + F+  E  FY +      +  RE  +   +K 
Sbjct: 946 MLRRTKGSLLDGKPIISLPPKSIELRKVDFTVEERDFYSKLEAESRTQFREYAEAGTVKQ 962

Query: 780 DFVKRKVADCVSSDAPSDILITHTDAGKLLSTLLKLRQACCHP 794
           ++V                         +L  LL+LRQAC HP
Sbjct: 1006 NYV------------------------NILLMLLRLRQACDHP 962

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134046.10.0e+0099.04E3 ubiquitin-protein ligase SHPRH [Momordica charantia] >XP_022134047.1 E3 ubiqu... [more]
XP_038884903.10.0e+0086.10E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1... [more]
XP_023551402.10.0e+0085.45E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022938761.10.0e+0085.10E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucurbita moschata][more]
KAG6578487.10.0e+0084.92E3 ubiquitin-protein ligase SHPRH, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q149N81.2e-14630.65E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2[more]
Q7TPQ33.2e-14430.19E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1[more]
Q9UTL96.4e-6023.10Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (str... [more]
Q9FNI63.9e-4127.05DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1[more]
Q9FIY72.4e-3827.53DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BYG60.0e+0099.04E3 ubiquitin-protein ligase SHPRH OS=Momordica charantia OX=3673 GN=LOC111006415... [more]
A0A6J1FE160.0e+0085.10E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JXA70.0e+0084.63E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3C3N10.0e+0084.73E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A0A0K8S10.0e+0084.44RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 P... [more]
Match NameE-valueIdentityDescription
AT2G40770.10.0e+0056.73zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding [more]
AT5G22750.12.8e-4227.05DNA/RNA helicase protein [more]
AT5G43530.11.7e-3927.53Helicase protein with RING/U-box domain [more]
AT1G61140.11.9e-2724.62SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... [more]
AT1G61140.21.9e-2724.62SNF2 domain-containing protein / helicase domain-containing protein / zinc finge... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1020..1040
NoneNo IPR availablePANTHERPTHR45865E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBERcoord: 1..1652
NoneNo IPR availableCDDcd18070DEXQc_SHPRHcoord: 291..699
e-value: 2.59625E-88
score: 286.547
NoneNo IPR availableCDDcd18793SF2_C_SNFcoord: 1450..1592
e-value: 2.66914E-34
score: 126.822
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 1350..1413
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 287..668
e-value: 2.7E-14
score: 63.5
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 417..493
e-value: 7.0E-4
score: 28.9
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 1484..1581
e-value: 0.008
score: 24.0
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 1458..1581
e-value: 2.1E-5
score: 24.9
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 1360..1408
e-value: 2.1E-5
score: 34.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 1360..1409
score: 11.456568
IPR000330SNF2, N-terminalPFAMPF00176SNF2_Ncoord: 294..795
e-value: 1.0E-85
score: 287.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1426..1672
e-value: 2.2E-38
score: 134.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 707..1648
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 271..696
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 408..460
e-value: 1.4E-10
score: 42.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1343..1425
e-value: 2.7E-9
score: 38.1
IPR038718SNF2-like, N-terminal domain superfamilyGENE3D3.40.50.10810coord: 345..399
e-value: 1.2E-5
score: 26.2
IPR038718SNF2-like, N-terminal domain superfamilyGENE3D3.40.50.10810coord: 490..721
e-value: 2.6E-29
score: 103.8
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 1376..1385
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 413..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003347.1MS003347.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding