MS003332 (gene) Bitter gourd (TR) v1

BLAST of MS003332 vs. NCBI nr
Match: KAA0039372.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 932.9 bits (2410), Expect = 1.2e-267
Identity = 463/523 (88.53%), Postives = 492/523 (94.07%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+ + LTT+AL +T  F IFS  YDTV CY LTPRRIK+ MAKQGV GPKPRPLLGNIL
Sbjct: 3   IMLPLLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVA+LLSKSTS DMPSISHD+VPRLLPHFLAWS+QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYS VSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTKEML+SIE EVKSGRSEFEIGEYMT LTADII+RTEFESS+EKGKQIFHLLT+LQHLC
Sbjct: 183 CTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLC PGSRFFPSKYNREIK LKGKVEELLMEII+SR++CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KKKL GN  LS LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQDK
Sbjct: 303 LLNEMQ-KKKLDGNNGLS-LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAEVM+VC   TP+ HHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS+ELWGKDANEFNPERFANSK FTSG FIPFA+GPRNC+GQSFALMET
Sbjct: 423 LSIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS NYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 483 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of MS003332 vs. NCBI nr
Match: XP_008459472.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 931.8 bits (2407), Expect = 2.6e-267
Identity = 462/523 (88.34%), Postives = 492/523 (94.07%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+ + LTT+AL +T  F IFS  YDTV CY LTPRRIK+ MAKQGV GPKPRPLLGNIL
Sbjct: 3   IMLPLLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVA+LLSKSTS DMPSISHD+VPRLLPHFLAWS+QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYS VSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTKEML+SIE EVKSGRSEFEIGEYMT LTADII+RTEFESS+EKGKQIFHLLT+LQHLC
Sbjct: 183 CTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLC PGSRFFPSKYNREIK LKGKVEELLMEII+SR++CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KKKL GN  LS LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQDK
Sbjct: 303 LLNEMQ-KKKLDGNNGLS-LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAEVM+VC   TP+ HHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIP+LAIHHS+ELWGKDANEFNPERFANSK FTSG FIPFA+GPRNC+GQSFALMET
Sbjct: 423 LSIWIPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS NYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 483 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of MS003332 vs. NCBI nr
Match: XP_011656036.1 (cytokinin hydroxylase [Cucumis sativus] >KAE8648965.1 hypothetical protein Csa_008948 [Cucumis sativus])

HSP 1 Score: 927.5 bits (2396), Expect = 4.9e-266
Identity = 461/523 (88.15%), Postives = 492/523 (94.07%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+   LTT+AL +T  F IFS  YDT+ CY  TPRRIK+ MAKQGV GPKPRPLLGNIL
Sbjct: 3   IMLLPLLTTLAL-LTTSFFIFSLLYDTLSCYLFTPRRIKQKMAKQGVHGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVA+LLSKSTS DMPSISHD+VPRLLPHFLAWS+QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYSAVSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTKEML+S+E EVKSGRSEFEIGEYMT LTADII+RTEFESS+EKGKQIFHLLT+LQHLC
Sbjct: 183 CTKEMLESLEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLCLPGSRFFPSKYNREIK LKGKVEELLMEII+SRR+CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KKKL GN  LS LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ+K
Sbjct: 303 LLNEMQ-KKKLDGNNGLS-LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQNK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAEVM+VC   TP+ HHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS+ELWGKDANEFNPERFANSK FTSG FIPFA+GPRNCVGQSFALMET
Sbjct: 423 LSIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCVGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS +YRHAPVVVLTIKPKYGVQVCL+PLN
Sbjct: 483 KIILAMLISKFSFTISDSYRHAPVVVLTIKPKYGVQVCLEPLN 522

BLAST of MS003332 vs. NCBI nr
Match: XP_038890905.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 925.6 bits (2391), Expect = 1.9e-265
Identity = 459/523 (87.76%), Postives = 488/523 (93.31%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+ + LTT+ L +T  F IFS  YDT+ CY  TPRRIK  MAKQGVRGPKPRPLLGNIL
Sbjct: 3   IMLPLLLTTLVL-LTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVAALLSKSTS DMPSISHD+VPRLLPHFLAWS QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYSAVSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CT+EML+SIENEVKSGRSEFEIGEYMT LTADII+RTEFESSYEKGKQIFHLLT+LQHLC
Sbjct: 183 CTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLCLPGSRFFPSKYNREIK LKGKVEELLMEII+SRR+CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KK   GNG   +LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ K
Sbjct: 303 LLNEMQKKK--DGNG--LSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           +RAEV+++C   TP+ HHLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 LRAEVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS+E+WGKDANEFNPERFANSKAFTSG FIPFA+GPRNCVGQSFALMET
Sbjct: 423 LSIWIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS  YRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 483 KIILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 520

BLAST of MS003332 vs. NCBI nr
Match: XP_022992749.1 (cytokinin hydroxylase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 906.4 bits (2341), Expect = 1.2e-259
Identity = 445/521 (85.41%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 2   MVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNILD 61
           M+A+ LTT+A AVT  F IFS  Y T+ CYWLTPRRI + MAKQGVRGPKPRPLLGNILD
Sbjct: 4   MLALLLTTLAFAVT-SFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILD 63

Query: 62  VAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKEL 121
           VAALLSKSTS DMPSISHD+VPRLLPH+LAWSAQFGKRF+FWNG+EPRMCLT+T LIKEL
Sbjct: 64  VAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKEL 123

Query: 122 LSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVEC 181
           LSKYS+VSGKSWLQQ+GSKHFIGRGLLMANG NW HQRHIVAPAF+GD+LKSYAGYMVEC
Sbjct: 124 LSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVEC 183

Query: 182 TKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLCA 241
           T+EMLQS+ENEVKSGRSEFEIGEYMT LTADII+RTEFESSYEKGKQIFHLLT+LQHLCA
Sbjct: 184 TREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCA 243

Query: 242 QASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGML 301
           QASRHLCLPGSRFFPSKYNREIK LKGKVE LLMEII+SR++ VE+GRSSSYGNDLLGML
Sbjct: 244 QASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGML 303

Query: 302 LNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKV 361
           LNEMQ KK    NG   +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQD+V
Sbjct: 304 LNEMQKKK----NGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 363

Query: 362 RAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 421
           RAE+ +VCN  TP+LHHLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 364 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 423

Query: 422 SIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMETK 481
           S+WIPV+AIHHS+E+WGKDANEFNPERF +SKAFT G FIPFA+GPRNCVGQ+FALMETK
Sbjct: 424 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 483

Query: 482 IILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 484 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of MS003332 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 2.7e-211
Identity = 360/521 (69.10%), Postives = 435/521 (83.49%), Query Frame = 0

Query: 7   LTTVALAVTLVFI--IFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNILDVAA 66
           L T+  ++ ++F+  I    YDT+ CYWLTPRRIKK M +QGV GPKPRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 67  LLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKELLSK 126
           ++S+S S D  SI HD+V RLLPH++AWS Q+GKRF+ WNG +PR+CLT+T+LIKELL K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 127 YSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVECTKE 186
           ++ VSG+SWLQQ+G+K+FIGRGLLMANGQ+WHHQRH+ APAF G++LK YA +MVECT +
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 187 MLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLCAQAS 246
           +++ +  EV  G +E EIGE M  LTADII+RT+F SS+EKGK++F+ LT+LQ  CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 247 RHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGMLLNE 306
           RHLC PGSRF PSKYNREIK LK +VE LL+EII+SRR+C E+GRSS++G+DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 307 MQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKVRAE 366
           M + K    N   +N NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR E
Sbjct: 302 MDIDK----NNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREE 361

Query: 367 VMSVC--NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS 426
           V  V   NG  P++  LSKL+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLS
Sbjct: 362 VREVFGRNG-LPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLS 421

Query: 427 IWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR-FIPFAAGPRNCVGQSFALMETK 486
           IWIPVLAIHHS+ELWGKDAN+FNPERF   + F SGR FIPFAAGPRNC+GQ FALME K
Sbjct: 422 IWIPVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAK 481

Query: 487 IILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           IILA LISKF+FTIS+NYRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 482 IILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS003332 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 1.7e-205
Identity = 351/524 (66.98%), Postives = 426/524 (81.30%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           MMV + L  V   V ++ +I    YD++CCY+LTPRRIKK M +QG+ GPKPR L GNI+
Sbjct: 1   MMVTLVLKYV--LVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNII 60

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRF+ WNG EPR+CLT+T++IKE
Sbjct: 61  DISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKE 120

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LL+K++ V+GKSWLQQ+G+K FIGRGLLMANG+ WHHQRH+ APAF  D+LK YA +MVE
Sbjct: 121 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 180

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTK M + +  EV     E EIGE M  LTADII+RTEF SS +KGK++F LLT+LQ LC
Sbjct: 181 CTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 240

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQA+RHLC PGSRF PSKYNREIK LK +VE LLMEII+SR++ VE+GRSSSYG+DLLG+
Sbjct: 241 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGL 300

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLN+M   K        +NLN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQD 
Sbjct: 301 LLNQMDSNK--------NNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 360

Query: 361 VRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPK 420
           VR EV  VC     P++  LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPK
Sbjct: 361 VRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPK 420

Query: 421 GLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALME 480
           GLSIWIPVLAIHHS+ELWG+DANEFNPERF      +S  F+PFAAGPRNC+GQ+FA+ME
Sbjct: 421 GLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMME 480

Query: 481 TKIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
            KIILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 AKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of MS003332 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.3e-99
Identity = 207/529 (39.13%), Postives = 303/529 (57.28%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           ++  + L  +AL + +V  I+ A    V   W+  RR K    KQG+ GPK R L GN+ 
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFK----KQGISGPKYRILYGNLR 117

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           ++  + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C++D +L K+
Sbjct: 118 EIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQ 177

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           +LS       KS  + E  K   G GL+  NG +W   R I+ PAF  DKLK     MV+
Sbjct: 178 ILSNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVD 237

Query: 181 CTKEMLQSIENEVKSGRSE--FEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQH 240
           CT  M    + +     +E    I      LTADIIA   F SSY +G ++F     LQ 
Sbjct: 238 CTFRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQK 297

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLL 300
            CA A   L  PG ++ P+  N +I  L  KV   +  II++R        S  YGNDLL
Sbjct: 298 CCAAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLL 357

Query: 301 GMLLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ 360
           G++L           N     +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ
Sbjct: 358 GIMLT------AASSNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQ 417

Query: 361 DKVRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEI 420
           +K+R EV + C     P     SKL L++ V  ESLRLY P   L R+A ED+KLG+LEI
Sbjct: 418 EKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEI 477

Query: 421 PKGLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR---FIPFAAGPRNCVGQS 480
           PKG +I +P+  +H    +WG DA++FNP RFAN  +  +      + F+ GPR C+GQ+
Sbjct: 478 PKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQN 537

Query: 481 FALMETKIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           FA+ME K +LAM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 FAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of MS003332 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 7.7e-97
Identity = 201/532 (37.78%), Postives = 306/532 (57.52%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           M + + L  + L + +   IF A    V   ++  RR+K     QG+ GP  R   GN+ 
Sbjct: 1   MGLVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLK----NQGISGPNYRIFYGNLS 60

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           ++  +  +S    +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C++D +L K 
Sbjct: 61  EIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKT 120

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           +LS       KS  + E  K    +GL+   G +W   R I+ PAF  D+LK     MV+
Sbjct: 121 MLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVD 180

Query: 181 CTKEML-----QSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTL 240
           CT +ML     +S + E +  + + E+ E    LTADIIA + F SSY +G ++F     
Sbjct: 181 CTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQME 240

Query: 241 LQHLCAQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGN 300
           L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR +       S YG+
Sbjct: 241 LKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQ-----SKSDYGD 300

Query: 301 DLLGMLLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP 360
           DLLG+LL      K     G+   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ + 
Sbjct: 301 DLLGILL------KAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQ 360

Query: 361 SWQDKVRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGD 420
            WQ+K+R E+   C    TP     SKL L++MVI ESLRLY P S L R A  +IKLGD
Sbjct: 361 DWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGD 420

Query: 421 LEIPKGLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR---FIPFAAGPRNCV 480
           LEIPKG ++ IP+L +H    LWG DA++FNP RFAN  +  +      + F+ GPR C+
Sbjct: 421 LEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACI 480

Query: 481 GQSFALMETKIILAMLISKFSF-TISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           GQ+F ++E K +L M++ +F F ++   Y+H PV  +TI+P+YG+ V L+PL
Sbjct: 481 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of MS003332 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 5.0e-96
Identity = 184/503 (36.58%), Postives = 292/503 (58.05%), Query Frame = 0

Query: 32  WLTPRRIKKAMAKQGVRGPKPRPLLGNILDVAALLSKSTSDDMPSISHDVVPRLLPHFLA 91
           W  PR+I++  +KQG+RGP     +GN+ ++  ++ K++S  MP  SH+++PR+L  +  
Sbjct: 32  WWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHH 91

Query: 92  WSAQFGKRFVFWNGMEPRMCLTDTDLIKELLSKYSAVSGKSWLQQEGS---KHFIGRGLL 151
           W   +G  F+ W G   R+ + D DLI+E+ SK      + + + E     K   G GLL
Sbjct: 92  WRKIYGATFLVWFGPTFRLTVADPDLIREIFSK-----SEFYEKNEAHPLVKQLEGDGLL 151

Query: 152 MANGQNWHHQRHIVAPAFIGDKLKSYAGYMVECTKEMLQSIENEV-KSGRSEFEIGEYMT 211
              G+ W H R I++P F  + LK     +++   +M+    +++ ++G  E ++ E+  
Sbjct: 152 SLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQ 211

Query: 212 TLTADIIARTEFESSYEKGKQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKGLK 271
            LT D+I+RT F SSYE G+ +F L      LCA+A + + +PG RFFP++ N +   L 
Sbjct: 212 ILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLD 271

Query: 272 GKVEELLMEIIESRRECVEVGRSSS----YGNDLLGMLLNEMQMKKKLGGNGQLSNLNLQ 331
            ++ + L+++IE RR+    G           DLLG+++             Q  N+ +Q
Sbjct: 272 KEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI-------------QAKNVTVQ 331

Query: 332 IIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKVRAEVMSVCNG-TTPALHHLSKL 391
            I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC     P   H+ KL
Sbjct: 332 DIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKL 391

Query: 392 SLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSDELWGKDAN 451
             LSM++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+HH   +WG D N
Sbjct: 392 KTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVN 451

Query: 452 EFNPERFANSKAFTSGR---FIPFAAGPRNCVGQSFALMETKIILAMLISKFSFTISQNY 511
           EFNP RFA+     +     FIPF  G R C+GQ+ A+++ K+ LA++I +F+F ++  Y
Sbjct: 452 EFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTY 511

Query: 512 RHAPVVVLTIKPKYGVQVCLKPL 523
           +HAP V++ + P++G  +  + L
Sbjct: 512 QHAPTVLMLLYPQHGAPITFRRL 515

BLAST of MS003332 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.7e-268
Identity = 463/523 (88.53%), Postives = 492/523 (94.07%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+ + LTT+AL +T  F IFS  YDTV CY LTPRRIK+ MAKQGV GPKPRPLLGNIL
Sbjct: 3   IMLPLLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVA+LLSKSTS DMPSISHD+VPRLLPHFLAWS+QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYS VSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTKEML+SIE EVKSGRSEFEIGEYMT LTADII+RTEFESS+EKGKQIFHLLT+LQHLC
Sbjct: 183 CTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLC PGSRFFPSKYNREIK LKGKVEELLMEII+SR++CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KKKL GN  LS LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQDK
Sbjct: 303 LLNEMQ-KKKLDGNNGLS-LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAEVM+VC   TP+ HHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS+ELWGKDANEFNPERFANSK FTSG FIPFA+GPRNC+GQSFALMET
Sbjct: 423 LSIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS NYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 483 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of MS003332 vs. ExPASy TrEMBL
Match: A0A1S3CAS2 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.3e-267
Identity = 462/523 (88.34%), Postives = 492/523 (94.07%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+ + LTT+AL +T  F IFS  YDTV CY LTPRRIK+ MAKQGV GPKPRPLLGNIL
Sbjct: 3   IMLPLLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVA+LLSKSTS DMPSISHD+VPRLLPHFLAWS+QFGKRF+FWNG+EPRMCLT+TDLIKE
Sbjct: 63  DVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYS VSGKSWLQQ+GSKHFIGRGLLMANGQNW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTKEML+SIE EVKSGRSEFEIGEYMT LTADII+RTEFESS+EKGKQIFHLLT+LQHLC
Sbjct: 183 CTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLC PGSRFFPSKYNREIK LKGKVEELLMEII+SR++CVE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KKKL GN  LS LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQDK
Sbjct: 303 LLNEMQ-KKKLDGNNGLS-LNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDK 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAEVM+VC   TP+ HHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LSIWIP+LAIHHS+ELWGKDANEFNPERFANSK FTSG FIPFA+GPRNC+GQSFALMET
Sbjct: 423 LSIWIPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           KIILAMLISKFSFTIS NYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 483 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of MS003332 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.7e-260
Identity = 445/521 (85.41%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 2   MVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNILD 61
           M+A+ LTT+A AVT  F IFS  Y T+ CYWLTPRRI + MAKQGVRGPKPRPLLGNILD
Sbjct: 4   MLALLLTTLAFAVT-SFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILD 63

Query: 62  VAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKEL 121
           VAALLSKSTS DMPSISHD+VPRLLPH+LAWSAQFGKRF+FWNG+EPRMCLT+T LIKEL
Sbjct: 64  VAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKEL 123

Query: 122 LSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVEC 181
           LSKYS+VSGKSWLQQ+GSKHFIGRGLLMANG NW HQRHIVAPAF+GD+LKSYAGYMVEC
Sbjct: 124 LSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVEC 183

Query: 182 TKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLCA 241
           T+EMLQS+ENEVKSGRSEFEIGEYMT LTADII+RTEFESSYEKGKQIFHLLT+LQHLCA
Sbjct: 184 TREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCA 243

Query: 242 QASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGML 301
           QASRHLCLPGSRFFPSKYNREIK LKGKVE LLMEII+SR++ VE+GRSSSYGNDLLGML
Sbjct: 244 QASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGML 303

Query: 302 LNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKV 361
           LNEMQ KK    NG   +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQD+V
Sbjct: 304 LNEMQKKK----NGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 363

Query: 362 RAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 421
           RAE+ +VCN  TP+LHHLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 364 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 423

Query: 422 SIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMETK 481
           S+WIPV+AIHHS+E+WGKDANEFNPERF +SKAFT G FIPFA+GPRNCVGQ+FALMETK
Sbjct: 424 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 483

Query: 482 IILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 484 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of MS003332 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.6e-259
Identity = 444/522 (85.06%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           +M+A+ LTT+A A T  F IFS  Y T+ CYWLTPRRI + MAKQGVRGPKPRPLLGNIL
Sbjct: 3   IMLALLLTTLAFAAT-SFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNIL 62

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           DVAALLSKSTS DMPSISHD+VPRLLPH+LAWSAQFGKRF+FWNG+EPRMCLT+T LIKE
Sbjct: 63  DVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKE 122

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LLSKYS+VSGKSWLQQ+GSKHFIGRGLLMANG NW HQRHIVAPAF+GD+LKSYAGYMVE
Sbjct: 123 LLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CT+EMLQS+ENEVKSGRSEFEIGEYMT LTADII+RTEFESSYEKGKQIFHLLT+LQHLC
Sbjct: 183 CTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 242

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQASRHLCLPGSRFFPSKYNREIK LKGKVE LLMEII+SR++ VE+GRSSSYGNDLLGM
Sbjct: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGM 302

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLNEMQ KK    NG   +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQD+
Sbjct: 303 LLNEMQKKK----NGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDR 362

Query: 361 VRAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           VRAE+ +VCN  TP+LHHLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKG
Sbjct: 363 VRAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 422

Query: 421 LSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMET 480
           LS+WIPV+AIHHS+E+WGKDANEFNPERF +SKAFT G FIPFA+GPRNCVGQ+FALMET
Sbjct: 423 LSVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMET 482

Query: 481 KIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           KIILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 483 KIILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of MS003332 vs. ExPASy TrEMBL
Match: A0A6J1JWK8 (cytokinin hydroxylase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.6e-252
Identity = 437/521 (83.88%), Postives = 475/521 (91.17%), Query Frame = 0

Query: 2   MVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNILD 61
           M+A+ LTT+A AVT  F IFS  Y T+ CYWLTPRRI + MAKQGVRGPKPRPLLGNILD
Sbjct: 4   MLALLLTTLAFAVT-SFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILD 63

Query: 62  VAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKEL 121
           VAALLSKSTS DMPSISHD+VPRLLPH+LAWSAQFGKRF+FWNG+EPRMCLT+T LIKEL
Sbjct: 64  VAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKEL 123

Query: 122 LSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVEC 181
           LSKYS+VSGKSWLQQ+GSKHFIGRGLLMANG NW HQRHIVAPAF+GD+LKSYAGYMVEC
Sbjct: 124 LSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVEC 183

Query: 182 TKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLCA 241
           T+EMLQS+ENEVKSGRSEFEIGEYMT LTADII+RTEFESSYEKGKQIFHLLT+LQHLCA
Sbjct: 184 TREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCA 243

Query: 242 QASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGML 301
           QASRHLCLPGS        REIK LKGKVE LLMEII+SR++ VE+GRSSSYGNDLLGML
Sbjct: 244 QASRHLCLPGS--------REIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGML 303

Query: 302 LNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKV 361
           LNEMQ KK    NG   +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQD+V
Sbjct: 304 LNEMQKKK----NGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 363

Query: 362 RAEVMSVCNGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 421
           RAE+ +VCN  TP+LHHLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 364 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 423

Query: 422 SIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALMETK 481
           S+WIPV+AIHHS+E+WGKDANEFNPERF +SKAFT G FIPFA+GPRNCVGQ+FALMETK
Sbjct: 424 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 483

Query: 482 IILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 484 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 511

BLAST of MS003332 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 736.1 bits (1899), Expect = 2.0e-212
Identity = 360/521 (69.10%), Postives = 435/521 (83.49%), Query Frame = 0

Query: 7   LTTVALAVTLVFI--IFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNILDVAA 66
           L T+  ++ ++F+  I    YDT+ CYWLTPRRIKK M +QGV GPKPRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 67  LLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKELLSK 126
           ++S+S S D  SI HD+V RLLPH++AWS Q+GKRF+ WNG +PR+CLT+T+LIKELL K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 127 YSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVECTKE 186
           ++ VSG+SWLQQ+G+K+FIGRGLLMANGQ+WHHQRH+ APAF G++LK YA +MVECT +
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 187 MLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLCAQAS 246
           +++ +  EV  G +E EIGE M  LTADII+RT+F SS+EKGK++F+ LT+LQ  CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 247 RHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGMLLNE 306
           RHLC PGSRF PSKYNREIK LK +VE LL+EII+SRR+C E+GRSS++G+DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 307 MQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKVRAE 366
           M + K    N   +N NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR E
Sbjct: 302 MDIDK----NNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREE 361

Query: 367 VMSVC--NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS 426
           V  V   NG  P++  LSKL+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLS
Sbjct: 362 VREVFGRNG-LPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLS 421

Query: 427 IWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR-FIPFAAGPRNCVGQSFALMETK 486
           IWIPVLAIHHS+ELWGKDAN+FNPERF   + F SGR FIPFAAGPRNC+GQ FALME K
Sbjct: 422 IWIPVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAK 481

Query: 487 IILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           IILA LISKF+FTIS+NYRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 482 IILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS003332 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 716.8 bits (1849), Expect = 1.2e-206
Identity = 351/524 (66.98%), Postives = 426/524 (81.30%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           MMV + L  V   V ++ +I    YD++CCY+LTPRRIKK M +QG+ GPKPR L GNI+
Sbjct: 1   MMVTLVLKYV--LVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNII 60

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRF+ WNG EPR+CLT+T++IKE
Sbjct: 61  DISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKE 120

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           LL+K++ V+GKSWLQQ+G+K FIGRGLLMANG+ WHHQRH+ APAF  D+LK YA +MVE
Sbjct: 121 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 180

Query: 181 CTKEMLQSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQHLC 240
           CTK M + +  EV     E EIGE M  LTADII+RTEF SS +KGK++F LLT+LQ LC
Sbjct: 181 CTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 240

Query: 241 AQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLLGM 300
           AQA+RHLC PGSRF PSKYNREIK LK +VE LLMEII+SR++ VE+GRSSSYG+DLLG+
Sbjct: 241 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGL 300

Query: 301 LLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDK 360
           LLN+M   K        +NLN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQD 
Sbjct: 301 LLNQMDSNK--------NNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 360

Query: 361 VRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPK 420
           VR EV  VC     P++  LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPK
Sbjct: 361 VRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPK 420

Query: 421 GLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGRFIPFAAGPRNCVGQSFALME 480
           GLSIWIPVLAIHHS+ELWG+DANEFNPERF      +S  F+PFAAGPRNC+GQ+FA+ME
Sbjct: 421 GLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMME 480

Query: 481 TKIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
            KIILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 AKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of MS003332 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 365.2 bits (936), Expect = 9.1e-101
Identity = 207/529 (39.13%), Postives = 303/529 (57.28%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           ++  + L  +AL + +V  I+ A    V   W+  RR K    KQG+ GPK R L GN+ 
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFK----KQGISGPKYRILYGNLR 117

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           ++  + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C++D +L K+
Sbjct: 118 EIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQ 177

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           +LS       KS  + E  K   G GL+  NG +W   R I+ PAF  DKLK     MV+
Sbjct: 178 ILSNKFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVD 237

Query: 181 CTKEMLQSIENEVKSGRSE--FEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTLLQH 240
           CT  M    + +     +E    I      LTADIIA   F SSY +G ++F     LQ 
Sbjct: 238 CTFRMFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQK 297

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGNDLL 300
            CA A   L  PG ++ P+  N +I  L  KV   +  II++R        S  YGNDLL
Sbjct: 298 CCAAALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLL 357

Query: 301 GMLLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ 360
           G++L           N     +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ
Sbjct: 358 GIMLT------AASSNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQ 417

Query: 361 DKVRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEI 420
           +K+R EV + C     P     SKL L++ V  ESLRLY P   L R+A ED+KLG+LEI
Sbjct: 418 EKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEI 477

Query: 421 PKGLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR---FIPFAAGPRNCVGQS 480
           PKG +I +P+  +H    +WG DA++FNP RFAN  +  +      + F+ GPR C+GQ+
Sbjct: 478 PKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQN 537

Query: 481 FALMETKIILAMLISKFSFTISQNYRHAPVVVLTIKPKYGVQVCLKPLN 524
           FA+ME K +LAM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 FAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of MS003332 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 355.9 bits (912), Expect = 5.5e-98
Identity = 201/532 (37.78%), Postives = 306/532 (57.52%), Query Frame = 0

Query: 1   MMVAVALTTVALAVTLVFIIFSAAYDTVCCYWLTPRRIKKAMAKQGVRGPKPRPLLGNIL 60
           M + + L  + L + +   IF A    V   ++  RR+K     QG+ GP  R   GN+ 
Sbjct: 1   MGLVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLK----NQGISGPNYRIFYGNLS 60

Query: 61  DVAALLSKSTSDDMPSISHDVVPRLLPHFLAWSAQFGKRFVFWNGMEPRMCLTDTDLIKE 120
           ++  +  +S    +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C++D +L K 
Sbjct: 61  EIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKT 120

Query: 121 LLSKYSAVSGKSWLQQEGSKHFIGRGLLMANGQNWHHQRHIVAPAFIGDKLKSYAGYMVE 180
           +LS       KS  + E  K    +GL+   G +W   R I+ PAF  D+LK     MV+
Sbjct: 121 MLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVD 180

Query: 181 CTKEML-----QSIENEVKSGRSEFEIGEYMTTLTADIIARTEFESSYEKGKQIFHLLTL 240
           CT +ML     +S + E +  + + E+ E    LTADIIA + F SSY +G ++F     
Sbjct: 181 CTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQME 240

Query: 241 LQHLCAQASRHLCLPGSRFFPSKYNREIKGLKGKVEELLMEIIESRRECVEVGRSSSYGN 300
           L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR +       S YG+
Sbjct: 241 LKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQ-----SKSDYGD 300

Query: 301 DLLGMLLNEMQMKKKLGGNGQLSNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP 360
           DLLG+LL      K     G+   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ + 
Sbjct: 301 DLLGILL------KAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQ 360

Query: 361 SWQDKVRAEVMSVC-NGTTPALHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGD 420
            WQ+K+R E+   C    TP     SKL L++MVI ESLRLY P S L R A  +IKLGD
Sbjct: 361 DWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGD 420

Query: 421 LEIPKGLSIWIPVLAIHHSDELWGKDANEFNPERFANSKAFTSGR---FIPFAAGPRNCV 480
           LEIPKG ++ IP+L +H    LWG DA++FNP RFAN  +  +      + F+ GPR C+
Sbjct: 421 LEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACI 480

Query: 481 GQSFALMETKIILAMLISKFSF-TISQNYRHAPVVVLTIKPKYGVQVCLKPL 523
           GQ+F ++E K +L M++ +F F ++   Y+H PV  +TI+P+YG+ V L+PL
Sbjct: 481 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of MS003332 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 353.2 bits (905), Expect = 3.6e-97
Identity = 184/503 (36.58%), Postives = 292/503 (58.05%), Query Frame = 0

Query: 32  WLTPRRIKKAMAKQGVRGPKPRPLLGNILDVAALLSKSTSDDMPSISHDVVPRLLPHFLA 91
           W  PR+I++  +KQG+RGP     +GN+ ++  ++ K++S  MP  SH+++PR+L  +  
Sbjct: 32  WWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHH 91

Query: 92  WSAQFGKRFVFWNGMEPRMCLTDTDLIKELLSKYSAVSGKSWLQQEGS---KHFIGRGLL 151
           W   +G  F+ W G   R+ + D DLI+E+ SK      + + + E     K   G GLL
Sbjct: 92  WRKIYGATFLVWFGPTFRLTVADPDLIREIFSK-----SEFYEKNEAHPLVKQLEGDGLL 151

Query: 152 MANGQNWHHQRHIVAPAFIGDKLKSYAGYMVECTKEMLQSIENEV-KSGRSEFEIGEYMT 211
              G+ W H R I++P F  + LK     +++   +M+    +++ ++G  E ++ E+  
Sbjct: 152 SLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQ 211

Query: 212 TLTADIIARTEFESSYEKGKQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKGLK 271
            LT D+I+RT F SSYE G+ +F L      LCA+A + + +PG RFFP++ N +   L 
Sbjct: 212 ILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLD 271

Query: 272 GKVEELLMEIIESRRECVEVGRSSS----YGNDLLGMLLNEMQMKKKLGGNGQLSNLNLQ 331
            ++ + L+++IE RR+    G           DLLG+++             Q  N+ +Q
Sbjct: 272 KEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI-------------QAKNVTVQ 331

Query: 332 IIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQDKVRAEVMSVCNG-TTPALHHLSKL 391
            I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC     P   H+ KL
Sbjct: 332 DIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKL 391

Query: 392 SLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSDELWGKDAN 451
             LSM++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+HH   +WG D N
Sbjct: 392 KTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVN 451

Query: 452 EFNPERFANSKAFTSGR---FIPFAAGPRNCVGQSFALMETKIILAMLISKFSFTISQNY 511
           EFNP RFA+     +     FIPF  G R C+GQ+ A+++ K+ LA++I +F+F ++  Y
Sbjct: 452 EFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTY 511

Query: 512 RHAPVVVLTIKPKYGVQVCLKPL 523
           +HAP V++ + P++G  +  + L
Sbjct: 512 QHAPTVLMLLYPQHGAPITFRRL 515