MS003320 (gene) Bitter gourd (TR) v1

BLAST of MS003320 vs. NCBI nr
Match: XP_022134497.1 (probable transmembrane ascorbate ferrireductase 2 [Momordica charantia])

HSP 1 Score: 449.1 bits (1154), Expect = 2.3e-122
Identity = 230/232 (99.14%), Postives = 231/232 (99.57%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVPVVQFPIFMAVRVLGMIVA LVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL
Sbjct: 1   MAVPVVQFPIFMAVRVLGMIVATLVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT
Sbjct: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQTSKVILRYSNEALLINSLGILLVLLGGLVILA+IAPPNGKGEILRGPAE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAVIAPPNGKGEILRGPAE 232

BLAST of MS003320 vs. NCBI nr
Match: KAG6602565.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 418.3 bits (1074), Expect = 4.3e-113
Identity = 207/232 (89.22%), Postives = 221/232 (95.26%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGMIVA LV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 34  MAVPAVQFPIFMAVRVLGMIVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP NGKG+I RGP+E
Sbjct: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of MS003320 vs. NCBI nr
Match: KAG7033242.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 417.9 bits (1073), Expect = 5.6e-113
Identity = 206/232 (88.79%), Postives = 221/232 (95.26%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGM+VA LV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 33  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 92

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 93  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 152

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 153 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 212

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP NGKG+I RGP+E
Sbjct: 213 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 264

BLAST of MS003320 vs. NCBI nr
Match: XP_022956458.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita moschata])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 205/232 (88.36%), Postives = 221/232 (95.26%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGM+VA LV +WT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 34  MAVPAVQFPIFMAVRVLGMVVATLVFMWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP NGKG+I RGP+E
Sbjct: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of MS003320 vs. NCBI nr
Match: XP_023541096.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.8 bits (1065), Expect = 4.7e-112
Identity = 205/232 (88.36%), Postives = 220/232 (94.83%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGM+VA LV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP + KG+I RGPAE
Sbjct: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 265

BLAST of MS003320 vs. ExPASy Swiss-Prot
Match: Q9SWS1 (Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB561B PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.0e-89
Identity = 159/228 (69.74%), Postives = 192/228 (84.21%), Query Frame = 0

Query: 1   MAVPVV-QFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLV 60
           MAVPV+  FPIFM VRVLG I+AALV+ WT H+RGGLAL SDNKD IFNVHPV+MVIGL+
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 61  LLNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSW 120
           L NGEAMLAYK+  GTKN +KLVHL+LQ  AF LSLIGVWAALKFH DKGI+NFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 121 LGLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKA 180
           LGL  +FLF  QW AGFVT+WYPGGSRNSRA+L+PWHVF G+  Y L++ TA TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 181 TFLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEIL 228
           TFLQ ++VI RYS EA+L+N++G+L+++LGG VIL ++ P +GK ++L
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQVL 228

BLAST of MS003320 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 6.3e-51
Identity = 102/212 (48.11%), Postives = 141/212 (66.51%), Query Frame = 0

Query: 9   PIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVLLNGEAMLA 68
           P       L +  A +V++W   FRGGLA  +DNK+LIFNVHPVLM+IG ++L  EA++ 
Sbjct: 11  PFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSEAIMI 70

Query: 69  YKT-ASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWLGLLSVFL 128
           YK       +  KL+HL L  +A  L  +G++ A KFHN+ GI N YSLHSWLG+ ++ L
Sbjct: 71  YKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTISL 130

Query: 129 FGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKATFLQTSKV 188
           +GIQW  GFV F+YPG + + R   LPWHV FG++ Y L++ATA  GLLEK TFLQ+S  
Sbjct: 131 YGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSSG- 190

Query: 189 ILRYSNEALLINSLGILLVLLGGLVILAIIAP 220
           + +Y  EA L+N  G+++ L G  V++A +AP
Sbjct: 191 LDKYGAEAFLVNFTGLVVALFGAAVVVAAVAP 221

BLAST of MS003320 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-50
Identity = 100/212 (47.17%), Postives = 145/212 (68.40%), Query Frame = 0

Query: 9   PIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVLLNGEAMLA 68
           P   A   L +  AA+V++W+  FRGGLA+ S NK+LIFNVHPVLM+IG V++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAIMV 70

Query: 69  YKT-ASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWLGLLSVFL 128
           Y+   +   +  KL+HL L  +A  L  +G++ A K HN+ GI N YSLHSW+G+ ++ L
Sbjct: 71  YRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 129 FGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKATFLQTSKV 188
           +GIQW  GFVTF++PG + N +  +LPWHV FG++ Y L++A A  G LEK TFL++S  
Sbjct: 131 YGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESSG- 190

Query: 189 ILRYSNEALLINSLGILLVLLGGLVILAIIAP 220
           + +Y  EA L+N   +++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of MS003320 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.1e-50
Identity = 97/212 (45.75%), Postives = 144/212 (67.92%), Query Frame = 0

Query: 9   PIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVLLNGEAMLA 68
           P   A   L +  AA+V++W  +FRGGLA+ + NK+LIFNVHPVLM+IG +++ GEA++ 
Sbjct: 11  PFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAIMV 70

Query: 69  YKT-ASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWLGLLSVFL 128
           Y+   +      KL+HL L  +A  L  +G++ A K HN+ GI N YSLHSW+G+ ++ L
Sbjct: 71  YRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITL 130

Query: 129 FGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKATFLQTSKV 188
           +GIQW  GFVTF++PG + N +  +LPWH+ FG++ Y L++A A  G LEK TFL++S  
Sbjct: 131 YGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESSG- 190

Query: 189 ILRYSNEALLINSLGILLVLLGGLVILAIIAP 220
           + +Y  EA L+N   +++VL G  V++A IAP
Sbjct: 191 LDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of MS003320 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.9e-49
Identity = 100/220 (45.45%), Postives = 143/220 (65.00%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           M + V   P       L +  A +V++W  HFRGGLA  + NK+LIFNVHPVLM+IG ++
Sbjct: 1   MGLGVRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYII 60

Query: 61  LNGEAMLAYKTASGTK-NFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSW 120
           L  EA++ YK     K +  KL+HL L  +A     +G++ A KFHN+ GI N YSLHSW
Sbjct: 61  LGSEAIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSW 120

Query: 121 LGLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKA 180
           LG+ ++ L+GIQW  GFV F++P  S + R  +LPWH+ FG++ Y L++ATA  G LEK 
Sbjct: 121 LGIGTICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKL 180

Query: 181 TFLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAP 220
           TFLQ+S  + +Y  EA L+N   +++VL G  V++A ++P
Sbjct: 181 TFLQSSG-LDKYGAEAFLVNFTALIVVLFGASVVVAAVSP 219

BLAST of MS003320 vs. ExPASy TrEMBL
Match: A0A6J1BZR9 (probable transmembrane ascorbate ferrireductase 2 OS=Momordica charantia OX=3673 GN=LOC111006724 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.1e-122
Identity = 230/232 (99.14%), Postives = 231/232 (99.57%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVPVVQFPIFMAVRVLGMIVA LVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL
Sbjct: 1   MAVPVVQFPIFMAVRVLGMIVATLVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT
Sbjct: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQTSKVILRYSNEALLINSLGILLVLLGGLVILA+IAPPNGKGEILRGPAE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAVIAPPNGKGEILRGPAE 232

BLAST of MS003320 vs. ExPASy TrEMBL
Match: A0A6J1GWW7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita moschata OX=3662 GN=LOC111458185 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.0e-113
Identity = 205/232 (88.36%), Postives = 221/232 (95.26%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGM+VA LV +WT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 34  MAVPAVQFPIFMAVRVLGMVVATLVFMWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP NGKG+I RGP+E
Sbjct: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

BLAST of MS003320 vs. ExPASy TrEMBL
Match: A0A6J1JNQ1 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111486970 PE=4 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.3e-112
Identity = 205/232 (88.36%), Postives = 220/232 (94.83%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP VQFPIFMAVRVLGM+VA LV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+WAALKFHNDKGI+NFYSLHSWL
Sbjct: 94  LNGEAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWL 153

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVFFGVY YGLSIATA TGLLEKAT
Sbjct: 154 GLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKAT 213

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILA+IAP + KG+I RGPAE
Sbjct: 214 FLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 265

BLAST of MS003320 vs. ExPASy TrEMBL
Match: A0A5A7TD04 (Putative transmembrane ascorbate ferrireductase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001910 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 206/232 (88.79%), Postives = 219/232 (94.40%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVPVVQFPIF+AVRVLGM+VAAL+ IWT HFRGGLALISDNKD IFNVHPVLMV GL+L
Sbjct: 1   MAVPVVQFPIFLAVRVLGMLVAALLFIWTLHFRGGLALISDNKDHIFNVHPVLMVTGLIL 60

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNF+KLVHL+LQFVAF LSLIG+WAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLTLQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+SVFLFGIQW+AGFVTFWYPGGSRNSRATLLPWHVFFGVY YGLSIAT  TGLLEKAT
Sbjct: 121 GLVSVFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGVYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQTSKVILRYSNEALLINSLG+L+VLLGGLVILA+IAP N KG+I RG AE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDINRGLAE 232

BLAST of MS003320 vs. ExPASy TrEMBL
Match: Q3LGX4 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.8e-110
Identity = 203/232 (87.50%), Postives = 220/232 (94.83%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAVP+VQFPIFMAVRVLGM+VAAL+ IWT HFRGGLALIS+NKD IFNVHPVLMVIGL+L
Sbjct: 1   MAVPLVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISENKDHIFNVHPVLMVIGLIL 60

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           LNGEAMLAYKT SGTKNF+KLVHL+LQFVAF LSLIG+WAALKFHNDKGIDNFYSLHSWL
Sbjct: 61  LNGEAMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGIWAALKFHNDKGIDNFYSLHSWL 120

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           GL+S+FLFGIQW+AGFVTFWYPGGSRNSRATLLPWHVFFG+Y YGLSIAT  TGLLEKAT
Sbjct: 121 GLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVFFGIYIYGLSIATTVTGLLEKAT 180

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEILRGPAE 233
           FLQTSKVILRYSNEALLINSLG+L+VLLGGLVILA+IAP N KG++ RG AE
Sbjct: 181 FLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIAPSNAKGDVNRGLAE 232

BLAST of MS003320 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 330.1 bits (845), Expect = 1.4e-90
Identity = 159/228 (69.74%), Postives = 192/228 (84.21%), Query Frame = 0

Query: 1   MAVPVV-QFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLV 60
           MAVPV+  FPIFM VRVLG I+AALV+ WT H+RGGLAL SDNKD IFNVHPV+MVIGL+
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 61  LLNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSW 120
           L NGEAMLAYK+  GTKN +KLVHL+LQ  AF LSLIGVWAALKFH DKGI+NFYSLHSW
Sbjct: 61  LFNGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSW 120

Query: 121 LGLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKA 180
           LGL  +FLF  QW AGFVT+WYPGGSRNSRA+L+PWHVF G+  Y L++ TA TG+LEK 
Sbjct: 121 LGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKV 180

Query: 181 TFLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIAPPNGKGEIL 228
           TFLQ ++VI RYS EA+L+N++G+L+++LGG VIL ++ P +GK ++L
Sbjct: 181 TFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVVTPVSGKDQVL 228

BLAST of MS003320 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 189.9 bits (481), Expect = 2.3e-48
Identity = 95/218 (43.58%), Postives = 139/218 (63.76%), Query Frame = 0

Query: 1   MAVPVVQFPIFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60
           MAV +    +      L +I A +V++W+  +RGGLA  + NK+LIFN+HPVLM+IG ++
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 61  LNGEAMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWL 120
           L GEA+++YK+    K  +KL+HL L  +A  L + G+ AA K HN+  I N YSLHSW+
Sbjct: 61  LGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWI 120

Query: 121 GLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKAT 180
           G+  + L+G QW   F+ F++PGGS N ++ LLPWH   G++ Y L++  AA G LEK T
Sbjct: 121 GIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLT 180

Query: 181 FLQTSKVILRYSNEALLINSLGILLVLLGGLVILAIIA 219
           FL+    + +Y +EA LIN   I+ +L G  V+L   A
Sbjct: 181 FLENGG-LDKYGSEAFLINFTAIITILFGAFVVLTASA 217

BLAST of MS003320 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 188.3 bits (477), Expect = 6.7e-48
Identity = 102/212 (48.11%), Postives = 142/212 (66.98%), Query Frame = 0

Query: 10  IFMAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFN-VHPVLMVIGLVLLNGEAMLA 69
           + +  R+ G++VA  V+ W        AL   N  L ++ +HP+LMVIG +L++GEA+L 
Sbjct: 11  LVLFARLSGLVVAVSVLYW--------ALFLPNLGLSYSTLHPLLMVIGFILVSGEAILI 70

Query: 70  YKTASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGI-DNFYSLHSWLGLLSVFL 129
           ++   G++  +K VHL LQ +A   ++ G+W   KFH  +G+  NFYSLHSW+GLLSV L
Sbjct: 71  HRWLPGSRKTKKAVHLWLQGMALASAVFGIWT--KFHYQRGVFANFYSLHSWMGLLSVSL 130

Query: 130 FGIQWTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKATFLQTSKV 189
           F  QW  GF++FW+ G  R +R T LPWHVF G+YTYGL+IATA TGLLEK TFLQT + 
Sbjct: 131 FAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRN 190

Query: 190 ILRYSNEALLINSLGILLVLLGGLVILAIIAP 220
           + R  +E++ +N LG+ L LLG +VI A I P
Sbjct: 191 VPRRGSESMTVNGLGLGLALLGCIVITAAILP 212

BLAST of MS003320 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 144.1 bits (362), Expect = 1.5e-34
Identity = 74/198 (37.37%), Postives = 111/198 (56.06%), Query Frame = 0

Query: 12  MAVRVLGMIVAALVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVLLNGEAMLAYKT 71
           +     G++   L++IW  H+R G+   SDN   + NVHP LM  G + L G+AM+ YKT
Sbjct: 19  LVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYKT 78

Query: 72  ASGTKNFRKLVHLSLQFVAFCLSLIGVWAALKFHNDKGIDNFYSLHSWLGLLSVFLFGIQ 131
           A  +   +K+VH  L  +   L ++G+ AA +FH+   + +  SLHSW+GL +  L G+Q
Sbjct: 79  AYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQ 138

Query: 132 WTAGFVTFWYPGGSRNSRATLLPWHVFFGVYTYGLSIATAATGLLEKATFLQTSKVILRY 191
           W  G  TF  P  S  +R  ++PWHV  G     + I  A TGL+++AT L  S      
Sbjct: 139 WLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQST----- 198

Query: 192 SNEALLINSLGILLVLLG 210
           + E+ LIN LG+ ++L G
Sbjct: 199 NAESRLINFLGLAILLFG 211