MS003291 (gene) Bitter gourd (TR) v1

Overview
NameMS003291
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase
Locationscaffold234: 1813825 .. 1815501 (-)
RNA-Seq ExpressionMS003291
SyntenyMS003291
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCTGTCTGCTGCTGTTTGAGTGTGGAGGATTATGAAGACTATGTTAATCCAAATAGTTCATTATATAGGAATTGCACCTGTCTTAGTTGCTTCATTCAGAACTTTTTAAATGCGGTAAGCCCATCTTTGATGCAATATGTTTCGGGTTATTTTTTTGCATGCCACTATCAAGCATGATAATACAAGTGTTCATGAACATATATATGGACGATGAGATTTTGTTGCAATGCTAATATTGGTGAAAATAGGAAGAAAAGTTTTTAAGGTCATATTTCGTATTTTGAAAAAAAAAAAAGACTCTTCCATAGATTGCAGAAAGTTCTGACTAAATAAACTCTTTCGTATTTTGTCATAACAATGTTGAAAAAAAACATCACTAATTGGTATTTATTATTTTATGAGAATTTGGTGTTTATATAGTACTAGAGTGAAGAGTCTATTGTTAAATATATTTTTCCTGAAAAATCTGTGTTGAAAATTGGCATTGCAAATATTAGCAGGAAAATGTCGTGAACATTAACATTATTTACTTCCTTGTATGCTATATTATTGTGCTGTGTGCTTGCATTTGTGTCCTTTTGACGTTCCTTTTTTTTTTAATAGATTAAGAGACGGATAATATATTAAAAAAATACTCCACTGGCAAACAACCTAACAGCCGAGGTAGAGGGAACCCCTGCCAATAGAATTGAACCAAAAAGACCTTCCAATCATTTACAATCATACCCAAACTATTGCCTATGCTTTATAATGCGTTAGTGTGGGTAATTACATATAACCTATGACTCACTACAAATTATAATATAATTATGGAAAAGCACTTGTAATTTGTTTCAAGTGTCAATATAGTTTGAGACTTATATCCTCAAGCTAATTGTGTTACTTTGTTTTTCCTATTATTTTCTCTAGTACACATTGATATTCCGAAGAGGAGAAGTGCATTCTGTCTCTTCATCAATTCAAGGCACAACATCTATGAATTCTACAGCATCAACTGATAACTCTTTATCTGAAATGTATCGCTCACCTCCAAGGCCGTTGCCTTATGATGCTGACCCCAGATACATCCGCTTGCAGCGAGATGGATTAATTTCTAGGCGAGAGAAGGGGTCAAGCCATTTACATGAGGAGTCAGAACCTTTAAGAAGTGAAGTAGATGCAGATTCAGATTCTTTGGACACGCCAGGCAAGTGGAATGGATCTGCTTGTCTTGATGAATCTAAAGAAAATCTTGGCAAGTCATCTCTTAAGTTTCCTTCAACAAAGTCTACCACTGGAGTTGGGTATGCTTATACATCGTCTGAAGATGAGGATGTTTGTCCGACGTGTCTTGAAGGTTGATTTATTTAATTAACTTCATTCTATCATGGATTTTATTTTATTGTAGATTTTGTTACTTCTAATAGTCCGTGGTTTTAGCCCTCGATATTCTTGTTTTTGAAGATCTGAGAGAGATTTACGTCCAAGTTACTGATCCACACATTTTTTGATGTACTGCCTCATTCTGCTCGTTTAATTTTCTTCTTTTTTGTATCAGAATACACTTCAGAGAATCCCAAGATAATGACACAATGTTCTCATCATTTTCACCTTGGATGTATCTATGAGTGGATGGAGAGAAGTGATAACTGTCCAGTTTGTGGCAAGGTATGGTTATTTTTCTCCATCATA

mRNA sequence

ATGGGTGCTGTCTGCTGCTGTTTGAGTGTGGAGGATTATGAAGACTATGTTAATCCAAATAGTTCATTATATAGGAATTGCACCTGTCTTAGTTGCTTCATTCAGAACTTTTTAAATGCGTACACATTGATATTCCGAAGAGGAGAAGTGCATTCTGTCTCTTCATCAATTCAAGGCACAACATCTATGAATTCTACAGCATCAACTGATAACTCTTTATCTGAAATGTATCGCTCACCTCCAAGGCCGTTGCCTTATGATGCTGACCCCAGATACATCCGCTTGCAGCGAGATGGATTAATTTCTAGGCGAGAGAAGGGGTCAAGCCATTTACATGAGGAGTCAGAACCTTTAAGAAGTGAAGTAGATGCAGATTCAGATTCTTTGGACACGCCAGGCAAGTGGAATGGATCTGCTTGTCTTGATGAATCTAAAGAAAATCTTGGCAAGTCATCTCTTAAGTTTCCTTCAACAAAGTCTACCACTGGAGTTGGGTATGCTTATACATCGTCTGAAGATGAGGATGTTTGTCCGACGTGTCTTGAAGAATACACTTCAGAGAATCCCAAGATAATGACACAATGTTCTCATCATTTTCACCTTGGATGTATCTATGAGTGGATGGAGAGAAGTGATAACTGTCCAGTTTGTGGCAAGGTATGGTTATTTTTCTCCATCATA

Coding sequence (CDS)

ATGGGTGCTGTCTGCTGCTGTTTGAGTGTGGAGGATTATGAAGACTATGTTAATCCAAATAGTTCATTATATAGGAATTGCACCTGTCTTAGTTGCTTCATTCAGAACTTTTTAAATGCGTACACATTGATATTCCGAAGAGGAGAAGTGCATTCTGTCTCTTCATCAATTCAAGGCACAACATCTATGAATTCTACAGCATCAACTGATAACTCTTTATCTGAAATGTATCGCTCACCTCCAAGGCCGTTGCCTTATGATGCTGACCCCAGATACATCCGCTTGCAGCGAGATGGATTAATTTCTAGGCGAGAGAAGGGGTCAAGCCATTTACATGAGGAGTCAGAACCTTTAAGAAGTGAAGTAGATGCAGATTCAGATTCTTTGGACACGCCAGGCAAGTGGAATGGATCTGCTTGTCTTGATGAATCTAAAGAAAATCTTGGCAAGTCATCTCTTAAGTTTCCTTCAACAAAGTCTACCACTGGAGTTGGGTATGCTTATACATCGTCTGAAGATGAGGATGTTTGTCCGACGTGTCTTGAAGAATACACTTCAGAGAATCCCAAGATAATGACACAATGTTCTCATCATTTTCACCTTGGATGTATCTATGAGTGGATGGAGAGAAGTGATAACTGTCCAGTTTGTGGCAAGGTATGGTTATTTTTCTCCATCATA

Protein sequence

MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGTTSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRSEVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLFFSII
Homology
BLAST of MS003291 vs. NCBI nr
Match: XP_022134111.1 (E3 ubiquitin-protein ligase At3g02290 [Momordica charantia] >XP_022134112.1 E3 ubiquitin-protein ligase At3g02290 [Momordica charantia])

HSP 1 Score: 460.3 bits (1183), Expect = 9.6e-126
Identity = 221/223 (99.10%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT
Sbjct: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC
Sbjct: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKV  F
Sbjct: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVMAF 223

BLAST of MS003291 vs. NCBI nr
Match: XP_038889911.1 (E3 ubiquitin-protein ligase At3g02290 [Benincasa hispida])

HSP 1 Score: 438.0 bits (1125), Expect = 5.1e-119
Identity = 208/223 (93.27%), Postives = 218/223 (97.76%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVED+EDYVNPNSS+YRNCTCLSCFIQ+FL+AYTLIFRRGEVHSVSSSIQGT
Sbjct: 1   MGAVCCCLSVEDFEDYVNPNSSVYRNCTCLSCFIQSFLSAYTLIFRRGEVHSVSSSIQGT 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           +VD DSDSL + GKWNGSACL+ESKENLGKSSLKFPSTKSTTG+GYAYTSSEDEDVCPTC
Sbjct: 121 DVDTDSDSLGSAGKWNGSACLEESKENLGKSSLKFPSTKSTTGLGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+CSHHFHLGCIYEWMERSDNCPVCGKV  F
Sbjct: 181 LEEYTSENPKIVTKCSHHFHLGCIYEWMERSDNCPVCGKVMAF 223

BLAST of MS003291 vs. NCBI nr
Match: XP_008459404.1 (PREDICTED: E3 ubiquitin-protein ligase At3g02290 [Cucumis melo])

HSP 1 Score: 436.0 bits (1120), Expect = 1.9e-118
Identity = 207/223 (92.83%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVED+EDYVNPNSS+YRNCTCLSCFIQ+FLNAYTLIFRRGEVHSVSSSIQG 
Sbjct: 1   MGAVCCCLSVEDFEDYVNPNSSVYRNCTCLSCFIQSFLNAYTLIFRRGEVHSVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GKWNGSACL+ESKENLGKSSLKFPSTKST+G+GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSAGKWNGSACLEESKENLGKSSLKFPSTKSTSGLGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+CSHHFHLGCIYEWMERSDNCPVCGK   F
Sbjct: 181 LEEYTSENPKIVTKCSHHFHLGCIYEWMERSDNCPVCGKAMAF 223

BLAST of MS003291 vs. NCBI nr
Match: KAG7033220.1 (E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434.1 bits (1115), Expect = 7.4e-118
Identity = 206/227 (90.75%), Postives = 216/227 (95.15%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLS++D+EDYVNPNSS+YRNCTCLSCFIQNFLNAYTLIFRRGEVH VSSSIQG 
Sbjct: 1   MGAVCCCLSMDDFEDYVNPNSSVYRNCTCLSCFIQNFLNAYTLIFRRGEVHPVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSM+S +STDNSLSE+YRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLR 
Sbjct: 61  TSMSSASSTDNSLSEIYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRG 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GKWNGSACLDESKENLGKSSLK PSTKSTTG GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSAGKWNGSACLDESKENLGKSSLKIPSTKSTTGAGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLFFSII 228
           LEEYTSENPKI+T+C HHFHLGCIYEWMERSDNCPVCGKV LFFSII
Sbjct: 181 LEEYTSENPKIVTKCCHHFHLGCIYEWMERSDNCPVCGKVCLFFSII 227

BLAST of MS003291 vs. NCBI nr
Match: XP_004141476.1 (E3 ubiquitin-protein ligase At3g02290 [Cucumis sativus] >KGN52530.1 hypothetical protein Csa_009250 [Cucumis sativus])

HSP 1 Score: 430.3 bits (1105), Expect = 1.1e-116
Identity = 206/223 (92.38%), Postives = 215/223 (96.41%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVED+EDYVNPNSS+YRNCTCLSCFIQ+FLNAYTLIFRRGEVHSVSSSIQG 
Sbjct: 1   MGAVCCCLSVEDFEDYVNPNSSVYRNCTCLSCFIQSFLNAYTLIFRRGEVHSVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GK NGSACL+ESKENLGKSSLKFPSTKST+G+GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSGGKRNGSACLEESKENLGKSSLKFPSTKSTSGLGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+CSHHFHLGCIYEWMERSDNCPVCGK   F
Sbjct: 181 LEEYTSENPKIVTKCSHHFHLGCIYEWMERSDNCPVCGKAMAF 223

BLAST of MS003291 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 8.3e-72
Identity = 140/231 (60.61%), Postives = 166/231 (71.86%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MG V  C  VED ++Y+NPNSS+YRNC C+ C   NFLN Y  +FRRGE  S+ SS+Q T
Sbjct: 1   MGCVSSCFRVEDIDEYMNPNSSVYRNCPCIRCLAHNFLNLYISVFRRGETRSLPSSVQAT 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
            S+ S++S DN LSE +RS PRPLPYDADPRY R     L+SRREKGSSH HEE EPLRS
Sbjct: 61  ASITSSSSHDNFLSEAFRSTPRPLPYDADPRYFR----SLVSRREKGSSHSHEEVEPLRS 120

Query: 121 EVDADSDSLDTPG-KW-NGSACL---DESKENLGKSSLKFPSTKSTTGVGYA---YTSSE 180
           + DADS+S    G KW N  + L   D  +E   KSSL+   ++S + +  +   Y  SE
Sbjct: 121 DSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADSENMYILSE 180

Query: 181 DEDVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           DEDVCPTCLEEYTSENPKI+T+CSHHFHL CIYEWMERS+NCPVCGKV  F
Sbjct: 181 DEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEF 227

BLAST of MS003291 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.1e-14
Identity = 29/52 (55.77%), Postives = 38/52 (73.08%), Query Frame = 0

Query: 172 EDEDVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           E+ED CP C E+Y  ENP++ T+C H FHL C+ EW+ERSD CP+C K  +F
Sbjct: 134 EEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVF 185

BLAST of MS003291 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-13
Identity = 29/45 (64.44%), Postives = 34/45 (75.56%), Query Frame = 0

Query: 175 DVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGK 220
           D CP CLEEY  +NPK++T+C H FHL CI  WMERS+ CPVC K
Sbjct: 102 DECPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVCDK 146

BLAST of MS003291 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 28/81 (34.57%), Postives = 45/81 (55.56%), Query Frame = 0

Query: 147 NLGKSSLKFPSTKSTTGVGYAYTSSED-----EDVCPTCLEEYTSENPKIMTQCSHHFHL 206
           NL  +S   P+  S++    A   + D     +D C  CLE +T ++P  +T C H +HL
Sbjct: 11  NLSDNSPFNPAIGSSSSSSSALVVASDDDNNTDDACSICLEPFTLQDPSTVTSCKHEYHL 70

Query: 207 GCIYEWMERSDNCPVCGKVWL 223
            CI EW +RS  CP+C ++++
Sbjct: 71  QCIIEWSQRSKECPICWQLFV 91

BLAST of MS003291 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.2e-08
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 0

Query: 174 EDVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVC 218
           +D C  CLE +   +P  +T C H +HL CI EW +RS  CP+C
Sbjct: 30  DDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMC 73

BLAST of MS003291 vs. ExPASy TrEMBL
Match: A0A6J1BYQ7 (E3 ubiquitin-protein ligase At3g02290 OS=Momordica charantia OX=3673 GN=LOC111006466 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.7e-126
Identity = 221/223 (99.10%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT
Sbjct: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC
Sbjct: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKV  F
Sbjct: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVMAF 223

BLAST of MS003291 vs. ExPASy TrEMBL
Match: A0A1S3CA28 (E3 ubiquitin-protein ligase At3g02290 OS=Cucumis melo OX=3656 GN=LOC103498549 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 9.4e-119
Identity = 207/223 (92.83%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVED+EDYVNPNSS+YRNCTCLSCFIQ+FLNAYTLIFRRGEVHSVSSSIQG 
Sbjct: 1   MGAVCCCLSVEDFEDYVNPNSSVYRNCTCLSCFIQSFLNAYTLIFRRGEVHSVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GKWNGSACL+ESKENLGKSSLKFPSTKST+G+GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSAGKWNGSACLEESKENLGKSSLKFPSTKSTSGLGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+CSHHFHLGCIYEWMERSDNCPVCGK   F
Sbjct: 181 LEEYTSENPKIVTKCSHHFHLGCIYEWMERSDNCPVCGKAMAF 223

BLAST of MS003291 vs. ExPASy TrEMBL
Match: A0A0A0KSH8 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G642120 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.2e-117
Identity = 206/223 (92.38%), Postives = 215/223 (96.41%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLSVED+EDYVNPNSS+YRNCTCLSCFIQ+FLNAYTLIFRRGEVHSVSSSIQG 
Sbjct: 1   MGAVCCCLSVEDFEDYVNPNSSVYRNCTCLSCFIQSFLNAYTLIFRRGEVHSVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS
Sbjct: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GK NGSACL+ESKENLGKSSLKFPSTKST+G+GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSGGKRNGSACLEESKENLGKSSLKFPSTKSTSGLGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+CSHHFHLGCIYEWMERSDNCPVCGK   F
Sbjct: 181 LEEYTSENPKIVTKCSHHFHLGCIYEWMERSDNCPVCGKAMAF 223

BLAST of MS003291 vs. ExPASy TrEMBL
Match: A0A6J1GR85 (E3 ubiquitin-protein ligase At3g02290 OS=Cucurbita moschata OX=3662 GN=LOC111456789 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.3e-115
Identity = 201/223 (90.13%), Postives = 211/223 (94.62%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLS++D+EDYVNPNSS+YRNCTCLSCFIQNFLNAYTLIFRRGEVH VSSSIQG 
Sbjct: 1   MGAVCCCLSMDDFEDYVNPNSSVYRNCTCLSCFIQNFLNAYTLIFRRGEVHPVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSM+S +STDNSLSE+YRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLR 
Sbjct: 61  TSMSSASSTDNSLSEIYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRG 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DSDSL + GKWNGSACLDESKENLGKSSLK PSTKSTTG GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSDSLGSAGKWNGSACLDESKENLGKSSLKIPSTKSTTGAGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+C HHFHLGCIYEWMERSDNCPVCGKV  F
Sbjct: 181 LEEYTSENPKIVTKCCHHFHLGCIYEWMERSDNCPVCGKVMAF 223

BLAST of MS003291 vs. ExPASy TrEMBL
Match: A0A6J1JPB8 (E3 ubiquitin-protein ligase At3g02290 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487128 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.8e-115
Identity = 200/223 (89.69%), Postives = 211/223 (94.62%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGAVCCCLS++D+EDYVNPNSS+YRNCTCLSCFIQNFLNAYTLIFRRGEVH VSSSIQG 
Sbjct: 1   MGAVCCCLSMDDFEDYVNPNSSVYRNCTCLSCFIQNFLNAYTLIFRRGEVHPVSSSIQGA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           TSM+S +STDNSLSE+YRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLR 
Sbjct: 61  TSMSSASSTDNSLSEIYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRG 120

Query: 121 EVDADSDSLDTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPTC 180
           EVD DS+SL + GKWNGSACLDESKENLGKSSLK PSTKSTTG GYAYTSSEDEDVCPTC
Sbjct: 121 EVDTDSESLGSAGKWNGSACLDESKENLGKSSLKIPSTKSTTGAGYAYTSSEDEDVCPTC 180

Query: 181 LEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           LEEYTSENPKI+T+C HHFHLGCIYEWMERSDNCPVCGKV  F
Sbjct: 181 LEEYTSENPKILTKCCHHFHLGCIYEWMERSDNCPVCGKVMAF 223

BLAST of MS003291 vs. TAIR 10
Match: AT3G02290.1 (RING/U-box superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 5.9e-73
Identity = 140/231 (60.61%), Postives = 166/231 (71.86%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MG V  C  VED ++Y+NPNSS+YRNC C+ C   NFLN Y  +FRRGE  S+ SS+Q T
Sbjct: 1   MGCVSSCFRVEDIDEYMNPNSSVYRNCPCIRCLAHNFLNLYISVFRRGETRSLPSSVQAT 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
            S+ S++S DN LSE +RS PRPLPYDADPRY R     L+SRREKGSSH HEE EPLRS
Sbjct: 61  ASITSSSSHDNFLSEAFRSTPRPLPYDADPRYFR----SLVSRREKGSSHSHEEVEPLRS 120

Query: 121 EVDADSDSLDTPG-KW-NGSACL---DESKENLGKSSLKFPSTKSTTGVGYA---YTSSE 180
           + DADS+S    G KW N  + L   D  +E   KSSL+   ++S + +  +   Y  SE
Sbjct: 121 DSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADSENMYILSE 180

Query: 181 DEDVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           DEDVCPTCLEEYTSENPKI+T+CSHHFHL CIYEWMERS+NCPVCGKV  F
Sbjct: 181 DEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEF 227

BLAST of MS003291 vs. TAIR 10
Match: AT5G15790.1 (RING/U-box superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 9.4e-71
Identity = 132/229 (57.64%), Postives = 163/229 (71.18%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MG V  C  V+D+EDY NP+SS+ R+C C  C + NFLN Y  +FRRGE  S+ SS+Q T
Sbjct: 1   MGCVSSCFGVDDFEDYPNPSSSVNRSCPCPRCLVNNFLNLYISLFRRGETRSLPSSLQAT 60

Query: 61  -TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLR 120
             S+ ++ S DN +S  + S PRPLPYDADPRY R +RD L+SRR+KGSSH HEE+EPLR
Sbjct: 61  NVSIATSTSYDNFMSNTFHSTPRPLPYDADPRYFRSRRDSLVSRRDKGSSHSHEEAEPLR 120

Query: 121 SEVDADSDSLDTPG-KWNGSACL--DESKENLGKSSLKF--PSTKSTTGVGYAYTSSEDE 180
           S+ D DS+S    G KW     +  ++SKE   +SS +     T ST+  G  Y +S+DE
Sbjct: 121 SDADVDSESFSVEGSKWANKLIISGEDSKEEFSRSSRRILQSRTMSTSNEG-LYITSDDE 180

Query: 181 DVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           DVCPTCLEEY SENPKI+T+CSHHFHL CIYEWMERS+NCPVCGKV  F
Sbjct: 181 DVCPTCLEEYISENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEF 228

BLAST of MS003291 vs. TAIR 10
Match: AT5G15790.2 (RING/U-box superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 9.4e-71
Identity = 132/229 (57.64%), Postives = 163/229 (71.18%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MG V  C  V+D+EDY NP+SS+ R+C C  C + NFLN Y  +FRRGE  S+ SS+Q T
Sbjct: 1   MGCVSSCFGVDDFEDYPNPSSSVNRSCPCPRCLVNNFLNLYISLFRRGETRSLPSSLQAT 60

Query: 61  -TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLR 120
             S+ ++ S DN +S  + S PRPLPYDADPRY R +RD L+SRR+KGSSH HEE+EPLR
Sbjct: 61  NVSIATSTSYDNFMSNTFHSTPRPLPYDADPRYFRSRRDSLVSRRDKGSSHSHEEAEPLR 120

Query: 121 SEVDADSDSLDTPG-KWNGSACL--DESKENLGKSSLKF--PSTKSTTGVGYAYTSSEDE 180
           S+ D DS+S    G KW     +  ++SKE   +SS +     T ST+  G  Y +S+DE
Sbjct: 121 SDADVDSESFSVEGSKWANKLIISGEDSKEEFSRSSRRILQSRTMSTSNEG-LYITSDDE 180

Query: 181 DVCPTCLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           DVCPTCLEEY SENPKI+T+CSHHFHL CIYEWMERS+NCPVCGKV  F
Sbjct: 181 DVCPTCLEEYISENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVMEF 228

BLAST of MS003291 vs. TAIR 10
Match: AT5G38895.1 (RING/U-box superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-68
Identity = 122/224 (54.46%), Postives = 155/224 (69.20%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGA CCC  V+ +E YVNPN+S+ RNC CL+CF+Q+F++ Y  +F RG +H + S+++  
Sbjct: 1   MGAFCCCFQVDLFESYVNPNTSITRNCPCLNCFLQSFMDLYASLFSRGGMHPIPSTVETA 60

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           T MNST + D+SLS +Y SPP PLPYDADPRY R           KGSSH  EESEPLR 
Sbjct: 61  TVMNSTTALDDSLSSVYHSPPTPLPYDADPRYFRF---------VKGSSHSGEESEPLRG 120

Query: 121 EVDADSDSL-DTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPT 180
           + +  S++L D   KW+ S   D SKE   K S  F  +K+  G+   Y  S+DED+CPT
Sbjct: 121 DTEMSSEALGDGGAKWSKSDSEDGSKEVYTKGSSTFTKSKTMPGIEVYYADSDDEDICPT 180

Query: 181 CLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           CL++YT ENPKI+T+CSHHFHL CIYEWMERS+ CPVCGKV  F
Sbjct: 181 CLDDYTLENPKIITKCSHHFHLSCIYEWMERSETCPVCGKVMAF 215

BLAST of MS003291 vs. TAIR 10
Match: AT5G38895.3 (RING/U-box superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-68
Identity = 122/224 (54.46%), Postives = 155/224 (69.20%), Query Frame = 0

Query: 1   MGAVCCCLSVEDYEDYVNPNSSLYRNCTCLSCFIQNFLNAYTLIFRRGEVHSVSSSIQGT 60
           MGA CCC  V+ +E YVNPN+S+ RNC CL+CF+Q+F++ Y  +F RG +H + S+++  
Sbjct: 106 MGAFCCCFQVDLFESYVNPNTSITRNCPCLNCFLQSFMDLYASLFSRGGMHPIPSTVETA 165

Query: 61  TSMNSTASTDNSLSEMYRSPPRPLPYDADPRYIRLQRDGLISRREKGSSHLHEESEPLRS 120
           T MNST + D+SLS +Y SPP PLPYDADPRY R           KGSSH  EESEPLR 
Sbjct: 166 TVMNSTTALDDSLSSVYHSPPTPLPYDADPRYFRF---------VKGSSHSGEESEPLRG 225

Query: 121 EVDADSDSL-DTPGKWNGSACLDESKENLGKSSLKFPSTKSTTGVGYAYTSSEDEDVCPT 180
           + +  S++L D   KW+ S   D SKE   K S  F  +K+  G+   Y  S+DED+CPT
Sbjct: 226 DTEMSSEALGDGGAKWSKSDSEDGSKEVYTKGSSTFTKSKTMPGIEVYYADSDDEDICPT 285

Query: 181 CLEEYTSENPKIMTQCSHHFHLGCIYEWMERSDNCPVCGKVWLF 224
           CL++YT ENPKI+T+CSHHFHL CIYEWMERS+ CPVCGKV  F
Sbjct: 286 CLDDYTLENPKIITKCSHHFHLSCIYEWMERSETCPVCGKVMAF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134111.19.6e-12699.10E3 ubiquitin-protein ligase At3g02290 [Momordica charantia] >XP_022134112.1 E3 u... [more]
XP_038889911.15.1e-11993.27E3 ubiquitin-protein ligase At3g02290 [Benincasa hispida][more]
XP_008459404.11.9e-11892.83PREDICTED: E3 ubiquitin-protein ligase At3g02290 [Cucumis melo][more]
KAG7033220.17.4e-11890.75E3 ubiquitin-protein ligase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_004141476.11.1e-11692.38E3 ubiquitin-protein ligase At3g02290 [Cucumis sativus] >KGN52530.1 hypothetical... [more]
Match NameE-valueIdentityDescription
Q8LE948.3e-7260.61E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022... [more]
Q2HIJ82.1e-1455.77Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q93ZF62.3e-1364.44E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ... [more]
Q4TU142.6e-0934.57E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ... [more]
Q9ZT422.2e-0845.45E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BYQ74.7e-12699.10E3 ubiquitin-protein ligase At3g02290 OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A1S3CA289.4e-11992.83E3 ubiquitin-protein ligase At3g02290 OS=Cucumis melo OX=3656 GN=LOC103498549 PE... [more]
A0A0A0KSH85.2e-11792.38RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G642120 P... [more]
A0A6J1GR851.3e-11590.13E3 ubiquitin-protein ligase At3g02290 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JPB82.8e-11589.69E3 ubiquitin-protein ligase At3g02290 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT3G02290.15.9e-7360.61RING/U-box superfamily protein [more]
AT5G15790.19.4e-7157.64RING/U-box superfamily protein [more]
AT5G15790.29.4e-7157.64RING/U-box superfamily protein [more]
AT5G38895.11.5e-6854.46RING/U-box superfamily protein [more]
AT5G38895.31.5e-6854.46RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 177..217
e-value: 1.0E-6
score: 38.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 177..217
score: 12.002429
IPR024766Zinc finger, RING-H2-typePFAMPF12678zf-rbx1coord: 174..217
e-value: 4.3E-11
score: 42.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 156..220
e-value: 2.3E-15
score: 57.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..132
NoneNo IPR availablePANTHERPTHR46463ZINC FINGER, RING/FYVE/PHD-TYPEcoord: 1..223
NoneNo IPR availablePANTHERPTHR46463:SF43TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 1..223
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 171..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003291.1MS003291.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008270 zinc ion binding