MS003260 (gene) Bitter gourd (TR) v1

Overview
NameMS003260
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCaM_binding domain-containing protein
Locationscaffold234: 1630541 .. 1632385 (+)
RNA-Seq ExpressionMS003260
SyntenyMS003260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAGAAGGGTGATGTGCTGCCAGTGACACCAGAAACGATCGTGGCTGCAGGTACTAATTTGAGAAGAAGCTCTATGGGGGGACAGACAAGTTCTTCGAATAGTGGAGAGAAACTTGTGCCTCATTATTTAAGAGCATCTACTGGCTCCTGTCATGATTTTTGCAAATATGGGAGAAAGCATGCTTTTGAAACCAAGACAAGACTTCCTATGCTAAAAAATGTGGCAAGTAAATCACTCGACAGTGAAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACCACCTCGACTCAGGCCCATGCCAAATTTTCATCAGCTTCCAGATTATCTGATACTGATTCGACCACCTTCGTAAGGCCTGAAATGTTGGTAAAGTCTTCTGTATTTCCAAGTCCAATCCAGAGAGAAGTCCTGAATGAAAGTAAGAAGAAATTGTTGGCCGGGCCAAGACCTTTCCCCGAGTCAAGATCACAATCTCCCATCGTTTTGAGACCAATGCAGAAAGAAGTCCTGAATGAAAGTAAGAAGAAGTTGTTGGTTGAGCCGAGACCTTTACCCAAGTCAAGATCACGGACTCCCGTCTTTTCGAGTCCAATGCAGAGAGAAGTCCTGAATGCAAGTAAGAAGAAATTATTGGCTGAGCCAAGACCTTCACTCAAGTCAAGATCACATACAACAAATGCCCTGAAAAACTTGAAGCCGAGAGCCTCTTCAGCTACTAGAAATCCAGAAGATGCTGTAGTTCAAGTTTTGGAAAAATCCAAGGAGAGAAGGTTACCTGAAAAGCATGATGACATTTCAAAATCGAAGTCTATTAAAGTGAAACCATTGAGATCTGCTGGGTCTCCAGAGAATTTGAGAAGAAAAAATGATTCCGAAGTAGGGAAAAGTTTAGTGACCTCCAAAGAAGCTGCTAAGAGAGTAGCAGCCTCCGCAAGAGCTTCACTATCCTCTAACTCTATCCGTGGAGCTGCAAACTTTACTGTGAGAAAGCGCAGTAACTTGAAAGCCGTCCCTCTTAAAACTCGTAGTAAGACGAAAATAGCTGAACGTGAGCAAGTCCAGAGTGAAGAGGTCCAAGAGAAAACCTTCCAGAGTGAAGAGGAGATTCAAGAAAAAACCTTCCAGATTGAAGAGGTCCAAGAGAAAACCTTATACGTCATTAAGATAGAAAATGAGGAGAAATCTCCACAATATGATCAGAATGAAACTGATGATAACATGGAAGCGGTGCCATTTTCACCACCTAAATCATTATCATCGCCACCGACCCCGTCTTTTTTGGCCAATGAAGAAGATCATGATGTATCTGAATACACAGAAAGTGAAGCAGAGGATGACTCTTACTCTGAAGACGATGAACTTGGCAGCATGGAAGCACATAATGTTTCAAGTGAAGGTGGTAAAGAAGTCGGATCCCACAATCTTGGGATGTATCAATCTGAAGGAAAGGATCCTCAATCTACAAAACTAAGTTTCAGAAGGGGAAAAATTATTGACATCCGGTCTGAGAGTAATAGTCCGAGGAGGCTTAAATTTAGGCGTGGAAGGCAACTGGGGGAGAATCAAAGGGCTGTTGATGGCTTTAGAAAAAACTTCAAGAAGGTAAAAGAAGTTGACAGTGACACCAACACCATAGAAACAGCTCCAGAAACTGTTGTTTTAAGGCACCAAGATGTGCAGGGGAAAAAAGATGCGCAGGGCTTGTTCAACAATGTTATTGAAGAAACCGCAAGTAAACTTGTTGAAACACGAAAGAGTAAGGTTAAGGCCTTGGTTGGTGCTTTTGAAACAGTGATCTCCCTGCAAGATGGGAAACCTTCTCTAGACACT

mRNA sequence

ATGGCCGAGAAGGGTGATGTGCTGCCAGTGACACCAGAAACGATCGTGGCTGCAGGTACTAATTTGAGAAGAAGCTCTATGGGGGGACAGACAAGTTCTTCGAATAGTGGAGAGAAACTTGTGCCTCATTATTTAAGAGCATCTACTGGCTCCTGTCATGATTTTTGCAAATATGGGAGAAAGCATGCTTTTGAAACCAAGACAAGACTTCCTATGCTAAAAAATGTGGCAAGTAAATCACTCGACAGTGAAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACCACCTCGACTCAGGCCCATGCCAAATTTTCATCAGCTTCCAGATTATCTGATACTGATTCGACCACCTTCGTAAGGCCTGAAATGTTGGTAAAGTCTTCTGTATTTCCAAGTCCAATCCAGAGAGAAGTCCTGAATGAAAGTAAGAAGAAATTGTTGGCCGGGCCAAGACCTTTCCCCGAGTCAAGATCACAATCTCCCATCGTTTTGAGACCAATGCAGAAAGAAGTCCTGAATGAAAGTAAGAAGAAGTTGTTGGTTGAGCCGAGACCTTTACCCAAGTCAAGATCACGGACTCCCGTCTTTTCGAGTCCAATGCAGAGAGAAGTCCTGAATGCAAGTAAGAAGAAATTATTGGCTGAGCCAAGACCTTCACTCAAGTCAAGATCACATACAACAAATGCCCTGAAAAACTTGAAGCCGAGAGCCTCTTCAGCTACTAGAAATCCAGAAGATGCTGTAGTTCAAGTTTTGGAAAAATCCAAGGAGAGAAGGTTACCTGAAAAGCATGATGACATTTCAAAATCGAAGTCTATTAAAGTGAAACCATTGAGATCTGCTGGGTCTCCAGAGAATTTGAGAAGAAAAAATGATTCCGAAGTAGGGAAAAGTTTAGTGACCTCCAAAGAAGCTGCTAAGAGAGTAGCAGCCTCCGCAAGAGCTTCACTATCCTCTAACTCTATCCGTGGAGCTGCAAACTTTACTGTGAGAAAGCGCAGTAACTTGAAAGCCGTCCCTCTTAAAACTCGTAGTAAGACGAAAATAGCTGAACGTGAGCAAGTCCAGAGTGAAGAGGTCCAAGAGAAAACCTTCCAGAGTGAAGAGGAGATTCAAGAAAAAACCTTCCAGATTGAAGAGGTCCAAGAGAAAACCTTATACGTCATTAAGATAGAAAATGAGGAGAAATCTCCACAATATGATCAGAATGAAACTGATGATAACATGGAAGCGGTGCCATTTTCACCACCTAAATCATTATCATCGCCACCGACCCCGTCTTTTTTGGCCAATGAAGAAGATCATGATGTATCTGAATACACAGAAAGTGAAGCAGAGGATGACTCTTACTCTGAAGACGATGAACTTGGCAGCATGGAAGCACATAATGTTTCAAGTGAAGGTGGTAAAGAAGTCGGATCCCACAATCTTGGGATGTATCAATCTGAAGGAAAGGATCCTCAATCTACAAAACTAAGTTTCAGAAGGGGAAAAATTATTGACATCCGGTCTGAGAGTAATAGTCCGAGGAGGCTTAAATTTAGGCGTGGAAGGCAACTGGGGGAGAATCAAAGGGCTGTTGATGGCTTTAGAAAAAACTTCAAGAAGGTAAAAGAAGTTGACAGTGACACCAACACCATAGAAACAGCTCCAGAAACTGTTGTTTTAAGGCACCAAGATGTGCAGGGGAAAAAAGATGCGCAGGGCTTGTTCAACAATGTTATTGAAGAAACCGCAAGTAAACTTGTTGAAACACGAAAGAGTAAGGTTAAGGCCTTGGTTGGTGCTTTTGAAACAGTGATCTCCCTGCAAGATGGGAAACCTTCTCTAGACACT

Coding sequence (CDS)

ATGGCCGAGAAGGGTGATGTGCTGCCAGTGACACCAGAAACGATCGTGGCTGCAGGTACTAATTTGAGAAGAAGCTCTATGGGGGGACAGACAAGTTCTTCGAATAGTGGAGAGAAACTTGTGCCTCATTATTTAAGAGCATCTACTGGCTCCTGTCATGATTTTTGCAAATATGGGAGAAAGCATGCTTTTGAAACCAAGACAAGACTTCCTATGCTAAAAAATGTGGCAAGTAAATCACTCGACAGTGAAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACCACCTCGACTCAGGCCCATGCCAAATTTTCATCAGCTTCCAGATTATCTGATACTGATTCGACCACCTTCGTAAGGCCTGAAATGTTGGTAAAGTCTTCTGTATTTCCAAGTCCAATCCAGAGAGAAGTCCTGAATGAAAGTAAGAAGAAATTGTTGGCCGGGCCAAGACCTTTCCCCGAGTCAAGATCACAATCTCCCATCGTTTTGAGACCAATGCAGAAAGAAGTCCTGAATGAAAGTAAGAAGAAGTTGTTGGTTGAGCCGAGACCTTTACCCAAGTCAAGATCACGGACTCCCGTCTTTTCGAGTCCAATGCAGAGAGAAGTCCTGAATGCAAGTAAGAAGAAATTATTGGCTGAGCCAAGACCTTCACTCAAGTCAAGATCACATACAACAAATGCCCTGAAAAACTTGAAGCCGAGAGCCTCTTCAGCTACTAGAAATCCAGAAGATGCTGTAGTTCAAGTTTTGGAAAAATCCAAGGAGAGAAGGTTACCTGAAAAGCATGATGACATTTCAAAATCGAAGTCTATTAAAGTGAAACCATTGAGATCTGCTGGGTCTCCAGAGAATTTGAGAAGAAAAAATGATTCCGAAGTAGGGAAAAGTTTAGTGACCTCCAAAGAAGCTGCTAAGAGAGTAGCAGCCTCCGCAAGAGCTTCACTATCCTCTAACTCTATCCGTGGAGCTGCAAACTTTACTGTGAGAAAGCGCAGTAACTTGAAAGCCGTCCCTCTTAAAACTCGTAGTAAGACGAAAATAGCTGAACGTGAGCAAGTCCAGAGTGAAGAGGTCCAAGAGAAAACCTTCCAGAGTGAAGAGGAGATTCAAGAAAAAACCTTCCAGATTGAAGAGGTCCAAGAGAAAACCTTATACGTCATTAAGATAGAAAATGAGGAGAAATCTCCACAATATGATCAGAATGAAACTGATGATAACATGGAAGCGGTGCCATTTTCACCACCTAAATCATTATCATCGCCACCGACCCCGTCTTTTTTGGCCAATGAAGAAGATCATGATGTATCTGAATACACAGAAAGTGAAGCAGAGGATGACTCTTACTCTGAAGACGATGAACTTGGCAGCATGGAAGCACATAATGTTTCAAGTGAAGGTGGTAAAGAAGTCGGATCCCACAATCTTGGGATGTATCAATCTGAAGGAAAGGATCCTCAATCTACAAAACTAAGTTTCAGAAGGGGAAAAATTATTGACATCCGGTCTGAGAGTAATAGTCCGAGGAGGCTTAAATTTAGGCGTGGAAGGCAACTGGGGGAGAATCAAAGGGCTGTTGATGGCTTTAGAAAAAACTTCAAGAAGGTAAAAGAAGTTGACAGTGACACCAACACCATAGAAACAGCTCCAGAAACTGTTGTTTTAAGGCACCAAGATGTGCAGGGGAAAAAAGATGCGCAGGGCTTGTTCAACAATGTTATTGAAGAAACCGCAAGTAAACTTGTTGAAACACGAAAGAGTAAGGTTAAGGCCTTGGTTGGTGCTTTTGAAACAGTGATCTCCCTGCAAGATGGGAAACCTTCTCTAGACACT

Protein sequence

MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGRKHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDSTTFVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGKPSLDT
Homology
BLAST of MS003260 vs. NCBI nr
Match: XP_022134206.1 (uncharacterized protein LOC111006524 [Momordica charantia] >XP_022134207.1 uncharacterized protein LOC111006524 [Momordica charantia] >XP_022134208.1 uncharacterized protein LOC111006524 [Momordica charantia] >XP_022134209.1 uncharacterized protein LOC111006524 [Momordica charantia] >XP_022134210.1 uncharacterized protein LOC111006524 [Momordica charantia])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 610/615 (99.19%), Postives = 612/615 (99.51%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDSTT 120
           KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTST+AHAKFSSASRLSDTDSTT
Sbjct: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTKAHAKFSSASRLSDTDSTT 120

Query: 121 FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKK 180
           FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRP PES SQSPIVLRPMQKEVLNESKK
Sbjct: 121 FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPLPESGSQSPIVLRPMQKEVLNESKK 180

Query: 181 KLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA 240
           KLL EPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA
Sbjct: 181 KLLFEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA 240

Query: 241 SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK 300
           SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK
Sbjct: 241 SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK 300

Query: 301 SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQS 360
           SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQV+S
Sbjct: 301 SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVRS 360

Query: 361 EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP 420
           EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP
Sbjct: 361 EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP 420

Query: 421 KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL 480
           KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL
Sbjct: 421 KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL 480

Query: 481 GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV 540
           GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV
Sbjct: 481 GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV 540

Query: 541 KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF 600
           KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF
Sbjct: 541 KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF 600

Query: 601 ETVISLQDGKPSLDT 616
           ETVISLQDGKPSLDT
Sbjct: 601 ETVISLQDGKPSLDT 615

BLAST of MS003260 vs. NCBI nr
Match: XP_004141582.3 (uncharacterized protein LOC101213671 isoform X3 [Cucumis sativus] >XP_031741331.1 uncharacterized protein LOC101213671 isoform X3 [Cucumis sativus] >KAE8648935.1 hypothetical protein Csa_008074 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.8e-174
Identity = 413/723 (57.12%), Postives = 470/723 (65.01%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV PVT ETI   GT  RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PVTLETIGTPGTYPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPML----------------------------------------------- 120
            H FETK+RLP+                                                
Sbjct: 61  NHGFETKSRLPLRKILGRKSLGGGISVDGIVLPERKNTTSTWASADFSSTSRLSETKSRL 120

Query: 121 ---KNVASKSLDSESSVDSVVLPERKKTTS------------------------------ 180
              KNVA KSLD   SVDSV+LPERKKTTS                              
Sbjct: 121 PLPKNVARKSLDGGKSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 180

Query: 181 ----------------TQAHAKFSSASRLSDTDSTTFVRPEMLVKSSVFPSPIQREVLNE 240
                           T+A ++ SS SRL + DSTTF +P++ V+S +FP+P+QRE+ N+
Sbjct: 181 SVDCVVFSERKNTTSTTRAKSELSSTSRLHEVDSTTFSKPKLPVESPIFPTPVQREIPNK 240

Query: 241 SKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKKKLLVEPRPLPKSRSRTPVFSSPMQ 300
            KKKLL+ P+                Q+EVLNE KKKLL                 SP Q
Sbjct: 241 RKKKLLSSPK----------------QREVLNERKKKLL----------------PSPKQ 300

Query: 301 REVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPEDAVVQVLEKSKERRLP 360
           REVLN  KKKLL EPR    SRS T N+LKNLKP A +ATR  ED+VVQV  K+K R LP
Sbjct: 301 REVLNERKKKLLVEPRTLPTSRSSTKNSLKNLKPEAFTATRRQEDSVVQVFAKAKGRELP 360

Query: 361 EKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGKSLVTSKEAAKRVAASARASLSSNS 420
           EK D I K KSIKVKPLRSAGS +N R+K+ S++GK L TSK AAK+V A++  S SSNS
Sbjct: 361 EKSDKILKPKSIKVKPLRSAGSLDNSRKKSYSKMGKCLETSKGAAKKVVAASTGSYSSNS 420

Query: 421 IRGAANFTVRKR-SNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSE---------EEI 480
           I GAAN T RK   NLK VPLK R+  K +ER QVQSEEVQEKTFQSE         EE+
Sbjct: 421 IHGAANLTARKHVGNLKGVPLKNRNMIKKSERGQVQSEEVQEKTFQSEEVQEETFQSEEV 480

Query: 481 QEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPPKSLSSPPTPSFLAN 540
           QEKT Q +E+QEKTLYVIKIENE+   Q DQ+ET+DNMEAV   PP+SLS P +P+ L N
Sbjct: 481 QEKTLQSKEIQEKTLYVIKIENEKIPQQPDQDETNDNMEAVSSLPPESLSPPISPALLPN 540

Query: 541 EEDHDVSEYTESEAEDDSYSEDDELGSMEAHN--VSSEGGKEVGSHNLGMYQSEGKDPQS 600
            ED DVSEYTESEAE+D Y E DE+GS E  +   SSEG +   S N G+  S+ KDP+S
Sbjct: 541 VEDQDVSEYTESEAENDFYYEGDEIGSSEPEDNITSSEGSENGRSRNHGILPSKEKDPRS 600

Query: 601 TKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEVDSDTNTIET 616
           TKLSFRRG++IDI SESNSPRRLKFRRGR LGENQ+A DG RKNFK+ KEVDS+TNT  T
Sbjct: 601 TKLSFRRGRVIDIHSESNSPRRLKFRRGRLLGENQKAGDGLRKNFKRGKEVDSETNT--T 660

BLAST of MS003260 vs. NCBI nr
Match: XP_031741329.1 (uncharacterized protein LOC101213671 isoform X1 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.8e-174
Identity = 413/723 (57.12%), Postives = 470/723 (65.01%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV PVT ETI   GT  RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 15  MAEKGDV-PVTLETIGTPGTYPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 74

Query: 61  KHAFETKTRLPML----------------------------------------------- 120
            H FETK+RLP+                                                
Sbjct: 75  NHGFETKSRLPLRKILGRKSLGGGISVDGIVLPERKNTTSTWASADFSSTSRLSETKSRL 134

Query: 121 ---KNVASKSLDSESSVDSVVLPERKKTTS------------------------------ 180
              KNVA KSLD   SVDSV+LPERKKTTS                              
Sbjct: 135 PLPKNVARKSLDGGKSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 194

Query: 181 ----------------TQAHAKFSSASRLSDTDSTTFVRPEMLVKSSVFPSPIQREVLNE 240
                           T+A ++ SS SRL + DSTTF +P++ V+S +FP+P+QRE+ N+
Sbjct: 195 SVDCVVFSERKNTTSTTRAKSELSSTSRLHEVDSTTFSKPKLPVESPIFPTPVQREIPNK 254

Query: 241 SKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKKKLLVEPRPLPKSRSRTPVFSSPMQ 300
            KKKLL+ P+                Q+EVLNE KKKLL                 SP Q
Sbjct: 255 RKKKLLSSPK----------------QREVLNERKKKLL----------------PSPKQ 314

Query: 301 REVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPEDAVVQVLEKSKERRLP 360
           REVLN  KKKLL EPR    SRS T N+LKNLKP A +ATR  ED+VVQV  K+K R LP
Sbjct: 315 REVLNERKKKLLVEPRTLPTSRSSTKNSLKNLKPEAFTATRRQEDSVVQVFAKAKGRELP 374

Query: 361 EKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGKSLVTSKEAAKRVAASARASLSSNS 420
           EK D I K KSIKVKPLRSAGS +N R+K+ S++GK L TSK AAK+V A++  S SSNS
Sbjct: 375 EKSDKILKPKSIKVKPLRSAGSLDNSRKKSYSKMGKCLETSKGAAKKVVAASTGSYSSNS 434

Query: 421 IRGAANFTVRKR-SNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSE---------EEI 480
           I GAAN T RK   NLK VPLK R+  K +ER QVQSEEVQEKTFQSE         EE+
Sbjct: 435 IHGAANLTARKHVGNLKGVPLKNRNMIKKSERGQVQSEEVQEKTFQSEEVQEETFQSEEV 494

Query: 481 QEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPPKSLSSPPTPSFLAN 540
           QEKT Q +E+QEKTLYVIKIENE+   Q DQ+ET+DNMEAV   PP+SLS P +P+ L N
Sbjct: 495 QEKTLQSKEIQEKTLYVIKIENEKIPQQPDQDETNDNMEAVSSLPPESLSPPISPALLPN 554

Query: 541 EEDHDVSEYTESEAEDDSYSEDDELGSMEAHN--VSSEGGKEVGSHNLGMYQSEGKDPQS 600
            ED DVSEYTESEAE+D Y E DE+GS E  +   SSEG +   S N G+  S+ KDP+S
Sbjct: 555 VEDQDVSEYTESEAENDFYYEGDEIGSSEPEDNITSSEGSENGRSRNHGILPSKEKDPRS 614

Query: 601 TKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEVDSDTNTIET 616
           TKLSFRRG++IDI SESNSPRRLKFRRGR LGENQ+A DG RKNFK+ KEVDS+TNT  T
Sbjct: 615 TKLSFRRGRVIDIHSESNSPRRLKFRRGRLLGENQKAGDGLRKNFKRGKEVDSETNT--T 674

BLAST of MS003260 vs. NCBI nr
Match: XP_031741330.1 (uncharacterized protein LOC101213671 isoform X2 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.8e-174
Identity = 413/723 (57.12%), Postives = 470/723 (65.01%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV PVT ETI   GT  RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 15  MAEKGDV-PVTLETIGTPGTYPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 74

Query: 61  KHAFETKTRLPML----------------------------------------------- 120
            H FETK+RLP+                                                
Sbjct: 75  NHGFETKSRLPLRKILGRKSLGGGISVDGIVLPERKNTTSTWASADFSSTSRLSETKSRL 134

Query: 121 ---KNVASKSLDSESSVDSVVLPERKKTTS------------------------------ 180
              KNVA KSLD   SVDSV+LPERKKTTS                              
Sbjct: 135 PLPKNVARKSLDGGKSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 194

Query: 181 ----------------TQAHAKFSSASRLSDTDSTTFVRPEMLVKSSVFPSPIQREVLNE 240
                           T+A ++ SS SRL + DSTTF +P++ V+S +FP+P+QRE+ N+
Sbjct: 195 SVDCVVFSERKNTTSTTRAKSELSSTSRLHEVDSTTFSKPKLPVESPIFPTPVQREIPNK 254

Query: 241 SKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKKKLLVEPRPLPKSRSRTPVFSSPMQ 300
            KKKLL+ P+                Q+EVLNE KKKLL                 SP Q
Sbjct: 255 RKKKLLSSPK----------------QREVLNERKKKLL----------------PSPKQ 314

Query: 301 REVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPEDAVVQVLEKSKERRLP 360
           REVLN  KKKLL EPR    SRS T N+LKNLKP A +ATR  ED+VVQV  K+K R LP
Sbjct: 315 REVLNERKKKLLVEPRTLPTSRSSTKNSLKNLKPEAFTATRRQEDSVVQVFAKAKGRELP 374

Query: 361 EKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGKSLVTSKEAAKRVAASARASLSSNS 420
           EK D I K KSIKVKPLRSAGS +N R+K+ S++GK L TSK AAK+V A++  S SSNS
Sbjct: 375 EKSDKILKPKSIKVKPLRSAGSLDNSRKKSYSKMGKCLETSKGAAKKVVAASTGSYSSNS 434

Query: 421 IRGAANFTVRKR-SNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSE---------EEI 480
           I GAAN T RK   NLK VPLK R+  K +ER QVQSEEVQEKTFQSE         EE+
Sbjct: 435 IHGAANLTARKHVGNLKGVPLKNRNMIKKSERGQVQSEEVQEKTFQSEEVQEETFQSEEV 494

Query: 481 QEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPPKSLSSPPTPSFLAN 540
           QEKT Q +E+QEKTLYVIKIENE+   Q DQ+ET+DNMEAV   PP+SLS P +P+ L N
Sbjct: 495 QEKTLQSKEIQEKTLYVIKIENEKIPQQPDQDETNDNMEAVSSLPPESLSPPISPALLPN 554

Query: 541 EEDHDVSEYTESEAEDDSYSEDDELGSMEAHN--VSSEGGKEVGSHNLGMYQSEGKDPQS 600
            ED DVSEYTESEAE+D Y E DE+GS E  +   SSEG +   S N G+  S+ KDP+S
Sbjct: 555 VEDQDVSEYTESEAENDFYYEGDEIGSSEPEDNITSSEGSENGRSRNHGILPSKEKDPRS 614

Query: 601 TKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEVDSDTNTIET 616
           TKLSFRRG++IDI SESNSPRRLKFRRGR LGENQ+A DG RKNFK+ KEVDS+TNT  T
Sbjct: 615 TKLSFRRGRVIDIHSESNSPRRLKFRRGRLLGENQKAGDGLRKNFKRGKEVDSETNT--T 674

BLAST of MS003260 vs. NCBI nr
Match: XP_008459364.1 (PREDICTED: uncharacterized protein LOC103498520 isoform X2 [Cucumis melo])

HSP 1 Score: 604.7 bits (1558), Expect = 8.6e-169
Identity = 404/680 (59.41%), Postives = 459/680 (67.50%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV P+T E I + GT+ RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PMTLEMIGSPGTHPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDS-- 120
            H FETK+RLP+ KN+  KSLD   SVDS+VLPERK  TST A A FSS SRLS+T S  
Sbjct: 61  NHGFETKSRLPVRKNLGRKSLDGGISVDSIVLPERKNPTSTWASADFSSTSRLSETKSRL 120

Query: 121 -----------------TTFVRPEMLVKSSVFPSPI--------QREVLNESKKKLLAGP 180
                             + + PE    +S + S          Q    N ++K L  G 
Sbjct: 121 PLPKNVARKSLDGGRSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 180

Query: 181 R----PFPESRS--------------------------------QSPIVLRPMQKEVLNE 240
                 F E ++                                +SPI   P+Q+EV NE
Sbjct: 181 SVDCVVFSERKNITSTTRAKSELSSTSRLYEADSTTFSKPKLPVESPIFPTPVQREVPNE 240

Query: 241 SKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLK 300
            KKKLL                SSP QREV+N  KKKLL E R    SRS T N+LKNLK
Sbjct: 241 RKKKLL----------------SSPKQREVVNERKKKLLDELRTLPTSRSSTKNSLKNLK 300

Query: 301 PRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSE 360
           P A +ATR  ED+VVQV  K+K R LPEK D I K +SIKVKPLRSAGS +N R+KN S 
Sbjct: 301 PEALAATRRQEDSVVQVFAKAKGRELPEKSDKILKPRSIKVKPLRSAGSLDNSRKKNHSN 360

Query: 361 VGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKR-SNLKAVPLKTRSKTKIAERE 420
           + K L TSKEAAK+V A +  S SSNSI GAAN T RK   NLK VPLK R+  KI+E  
Sbjct: 361 MRKWLETSKEAAKKVVAVSTGSYSSNSITGAANLTARKHVGNLKGVPLKNRNMIKISEHG 420

Query: 421 QVQSEEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVP 480
           QV+S EVQE+TFQS EE+QEKTFQ EEVQEKTLYVIKIENEE   Q DQ+ET+DNMEA P
Sbjct: 421 QVRSVEVQEETFQS-EEVQEKTFQSEEVQEKTLYVIKIENEEILQQPDQDETNDNMEAAP 480

Query: 481 FSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAH-NVSSEGGKEV 540
               +S+S P +P+ LAN ED DVSEYTESEAE+D Y E DE+GS EA  N SSEGG+  
Sbjct: 481 SLSSESISPPTSPALLANVEDQDVSEYTESEAENDFYYEGDEIGSSEAEDNASSEGGENG 540

Query: 541 GSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRK 600
            S N GM  S+ KDP+STK+SFRRG+IIDIRSE+NSPRRLKFRRGR L ENQ+A DG RK
Sbjct: 541 RSQNHGMLPSKEKDPRSTKVSFRRGRIIDIRSETNSPRRLKFRRGRLLAENQKAGDGLRK 600

Query: 601 NFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA 616
           NFK+ KEVD +TNT  TA ETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA
Sbjct: 601 NFKRGKEVDCETNT--TAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA 659

BLAST of MS003260 vs. ExPASy TrEMBL
Match: A0A6J1C1B9 (uncharacterized protein LOC111006524 OS=Momordica charantia OX=3673 GN=LOC111006524 PE=4 SV=1)

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 610/615 (99.19%), Postives = 612/615 (99.51%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDSTT 120
           KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTST+AHAKFSSASRLSDTDSTT
Sbjct: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTKAHAKFSSASRLSDTDSTT 120

Query: 121 FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKK 180
           FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRP PES SQSPIVLRPMQKEVLNESKK
Sbjct: 121 FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPLPESGSQSPIVLRPMQKEVLNESKK 180

Query: 181 KLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA 240
           KLL EPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA
Sbjct: 181 KLLFEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA 240

Query: 241 SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK 300
           SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK
Sbjct: 241 SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK 300

Query: 301 SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQS 360
           SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQV+S
Sbjct: 301 SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVRS 360

Query: 361 EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP 420
           EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP
Sbjct: 361 EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP 420

Query: 421 KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL 480
           KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL
Sbjct: 421 KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL 480

Query: 481 GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV 540
           GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV
Sbjct: 481 GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKV 540

Query: 541 KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF 600
           KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF
Sbjct: 541 KEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAF 600

Query: 601 ETVISLQDGKPSLDT 616
           ETVISLQDGKPSLDT
Sbjct: 601 ETVISLQDGKPSLDT 615

BLAST of MS003260 vs. ExPASy TrEMBL
Match: A0A1S3C9Z2 (uncharacterized protein LOC103498520 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498520 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 4.1e-169
Identity = 404/680 (59.41%), Postives = 459/680 (67.50%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV P+T E I + GT+ RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PMTLEMIGSPGTHPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDS-- 120
            H FETK+RLP+ KN+  KSLD   SVDS+VLPERK  TST A A FSS SRLS+T S  
Sbjct: 61  NHGFETKSRLPVRKNLGRKSLDGGISVDSIVLPERKNPTSTWASADFSSTSRLSETKSRL 120

Query: 121 -----------------TTFVRPEMLVKSSVFPSPI--------QREVLNESKKKLLAGP 180
                             + + PE    +S + S          Q    N ++K L  G 
Sbjct: 121 PLPKNVARKSLDGGRSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 180

Query: 181 R----PFPESRS--------------------------------QSPIVLRPMQKEVLNE 240
                 F E ++                                +SPI   P+Q+EV NE
Sbjct: 181 SVDCVVFSERKNITSTTRAKSELSSTSRLYEADSTTFSKPKLPVESPIFPTPVQREVPNE 240

Query: 241 SKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLK 300
            KKKLL                SSP QREV+N  KKKLL E R    SRS T N+LKNLK
Sbjct: 241 RKKKLL----------------SSPKQREVVNERKKKLLDELRTLPTSRSSTKNSLKNLK 300

Query: 301 PRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSE 360
           P A +ATR  ED+VVQV  K+K R LPEK D I K +SIKVKPLRSAGS +N R+KN S 
Sbjct: 301 PEALAATRRQEDSVVQVFAKAKGRELPEKSDKILKPRSIKVKPLRSAGSLDNSRKKNHSN 360

Query: 361 VGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKR-SNLKAVPLKTRSKTKIAERE 420
           + K L TSKEAAK+V A +  S SSNSI GAAN T RK   NLK VPLK R+  KI+E  
Sbjct: 361 MRKWLETSKEAAKKVVAVSTGSYSSNSITGAANLTARKHVGNLKGVPLKNRNMIKISEHG 420

Query: 421 QVQSEEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVP 480
           QV+S EVQE+TFQS EE+QEKTFQ EEVQEKTLYVIKIENEE   Q DQ+ET+DNMEA P
Sbjct: 421 QVRSVEVQEETFQS-EEVQEKTFQSEEVQEKTLYVIKIENEEILQQPDQDETNDNMEAAP 480

Query: 481 FSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAH-NVSSEGGKEV 540
               +S+S P +P+ LAN ED DVSEYTESEAE+D Y E DE+GS EA  N SSEGG+  
Sbjct: 481 SLSSESISPPTSPALLANVEDQDVSEYTESEAENDFYYEGDEIGSSEAEDNASSEGGENG 540

Query: 541 GSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRK 600
            S N GM  S+ KDP+STK+SFRRG+IIDIRSE+NSPRRLKFRRGR L ENQ+A DG RK
Sbjct: 541 RSQNHGMLPSKEKDPRSTKVSFRRGRIIDIRSETNSPRRLKFRRGRLLAENQKAGDGLRK 600

Query: 601 NFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA 616
           NFK+ KEVD +TNT  TA ETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA
Sbjct: 601 NFKRGKEVDCETNT--TAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKA 659

BLAST of MS003260 vs. ExPASy TrEMBL
Match: A0A1S3CA31 (uncharacterized protein LOC103498520 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498520 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 5.4e-169
Identity = 406/689 (58.93%), Postives = 460/689 (66.76%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV P+T E I + GT+ RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PMTLEMIGSPGTHPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDS-- 120
            H FETK+RLP+ KN+  KSLD   SVDS+VLPERK  TST A A FSS SRLS+T S  
Sbjct: 61  NHGFETKSRLPVRKNLGRKSLDGGISVDSIVLPERKNPTSTWASADFSSTSRLSETKSRL 120

Query: 121 -----------------TTFVRPEMLVKSSVFPSPI--------QREVLNESKKKLLAGP 180
                             + + PE    +S + S          Q    N ++K L  G 
Sbjct: 121 PLPKNVARKSLDGGRSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 180

Query: 181 R----PFPESRS--------------------------------QSPIVLRPMQKEVLNE 240
                 F E ++                                +SPI   P+Q+EV NE
Sbjct: 181 SVDCVVFSERKNITSTTRAKSELSSTSRLYEADSTTFSKPKLPVESPIFPTPVQREVPNE 240

Query: 241 SKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLK 300
            KKKLL                SSP QREV+N  KKKLL E R    SRS T N+LKNLK
Sbjct: 241 RKKKLL----------------SSPKQREVVNERKKKLLDELRTLPTSRSSTKNSLKNLK 300

Query: 301 PRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSE 360
           P A +ATR  ED+VVQV  K+K R LPEK D I K +SIKVKPLRSAGS +N R+KN S 
Sbjct: 301 PEALAATRRQEDSVVQVFAKAKGRELPEKSDKILKPRSIKVKPLRSAGSLDNSRKKNHSN 360

Query: 361 VGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKR-SNLKAVPLKTRSKTKIAERE 420
           + K L TSKEAAK+V A +  S SSNSI GAAN T RK   NLK VPLK R+  KI+E  
Sbjct: 361 MRKWLETSKEAAKKVVAVSTGSYSSNSITGAANLTARKHVGNLKGVPLKNRNMIKISEHG 420

Query: 421 QVQSEEVQEKTFQSE---------EEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNE 480
           QV+S EVQEKTFQSE         EE+QEKTFQ EEVQEKTLYVIKIENEE   Q DQ+E
Sbjct: 421 QVRSVEVQEKTFQSEEVQEETFQSEEVQEKTFQSEEVQEKTLYVIKIENEEILQQPDQDE 480

Query: 481 TDDNMEAVPFSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAH-N 540
           T+DNMEA P    +S+S P +P+ LAN ED DVSEYTESEAE+D Y E DE+GS EA  N
Sbjct: 481 TNDNMEAAPSLSSESISPPTSPALLANVEDQDVSEYTESEAENDFYYEGDEIGSSEAEDN 540

Query: 541 VSSEGGKEVGSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGEN 600
            SSEGG+   S N GM  S+ KDP+STK+SFRRG+IIDIRSE+NSPRRLKFRRGR L EN
Sbjct: 541 ASSEGGENGRSQNHGMLPSKEKDPRSTKVSFRRGRIIDIRSETNSPRRLKFRRGRLLAEN 600

Query: 601 QRAVDGFRKNFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLV 616
           Q+A DG RKNFK+ KEVD +TNT  TA ETVVLRHQDVQGKKDAQGLFNNVIEETASKLV
Sbjct: 601 QKAGDGLRKNFKRGKEVDCETNT--TAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLV 660

BLAST of MS003260 vs. ExPASy TrEMBL
Match: A0A5D3BNG0 (Plant calmodulin-binding protein-related, putative isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002570 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 6.0e-168
Identity = 402/689 (58.35%), Postives = 453/689 (65.75%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV P+T E I   GT+ RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PMTLEMIGTPGTHPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDS-- 120
            H FETK+RLP+ KN+  KSLD   S+DS+VLPERK  TST A A FSS SRLS+T S  
Sbjct: 61  NHGFETKSRLPVRKNLGRKSLDGGISLDSIVLPERKNPTSTWASADFSSTSRLSETKSRL 120

Query: 121 -----------------TTFVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPFPESRS 180
                             + + PE    +S + S  +       +     G R   E  S
Sbjct: 121 PLPKNVARKSLDGGRSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKTGARKSLEGGS 180

Query: 181 --------------------------------------------QSPIVLRPMQKEVLNE 240
                                                       +SPI   P+Q+EV NE
Sbjct: 181 SVDCVVFSERKNITSTTRAKSELSSTSRLYEADSTTFSKPKLPVESPIFPTPVQREVPNE 240

Query: 241 SKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLK 300
            KKKLL                SSP QREV+N  KKKLL E R    SRS T N+LKNLK
Sbjct: 241 RKKKLL----------------SSPKQREVVNERKKKLLDELRTLPTSRSSTKNSLKNLK 300

Query: 301 PRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSE 360
           P A +ATR  ED+VVQV  K+K R LPEK D I K +SIKVKPLRSAGS +N R+KN S 
Sbjct: 301 PEALAATRRQEDSVVQVFAKAKGRELPEKSDKILKPRSIKVKPLRSAGSLDNSRKKNHSN 360

Query: 361 VGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKR-SNLKAVPLKTRSKTKIAERE 420
           + K L TSKEAAK+V A +  S SSNSI GAAN T RK   NLK VPLK R+  KI+E  
Sbjct: 361 MRKWLETSKEAAKKVVAVSTGSYSSNSITGAANLTARKHVGNLKGVPLKNRNMIKISEHG 420

Query: 421 QVQSEEVQEKTFQSE---------EEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNE 480
           QV+S EVQEKTFQSE         EE+QEKTFQ EEVQEKTLYVIKIENEE   Q DQ+E
Sbjct: 421 QVRSVEVQEKTFQSEEVQEETFQSEEVQEKTFQSEEVQEKTLYVIKIENEEILQQPDQDE 480

Query: 481 TDDNMEAVPFSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAH-N 540
           T+DNMEA P    +S+S P +P  LAN ED DVSEYTESEAE+D Y E DE+GS EA  N
Sbjct: 481 TNDNMEAAPSLSSESISPPTSPVLLANVEDQDVSEYTESEAENDFYYEGDEIGSSEAEDN 540

Query: 541 VSSEGGKEVGSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGEN 600
            SSEGG+   S N GM  S+ KDP+STK+SFRRG+IIDIRSE+NSPRRLKFRRGR L EN
Sbjct: 541 ASSEGGENGRSQNHGMLPSKEKDPRSTKVSFRRGRIIDIRSETNSPRRLKFRRGRLLAEN 600

Query: 601 QRAVDGFRKNFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLV 616
           Q+A DG RKNFK+ KEVD +TNT  TA ETVVLRHQDVQGKKDAQGLFNNVIEETASKLV
Sbjct: 601 QKAGDGLRKNFKRGKEVDCETNT--TAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLV 660

BLAST of MS003260 vs. ExPASy TrEMBL
Match: A0A0A0KSH7 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638370 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 4.0e-164
Identity = 395/681 (58.00%), Postives = 450/681 (66.08%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAEKGDV PVT ETI   GT  RRSSM G TS SNS EKLVP+YLRASTGSCHDFCKYGR
Sbjct: 1   MAEKGDV-PVTLETIGTPGTYPRRSSM-GCTSYSNSVEKLVPNYLRASTGSCHDFCKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDS-- 120
            H FETK+RLP+ K +  KSL    SVD +VLPERK TTST A A FSS SRLS+T S  
Sbjct: 61  NHGFETKSRLPLRKILGRKSLGGGISVDGIVLPERKNTTSTWASADFSSTSRLSETKSRL 120

Query: 121 -----------------TTFVRPEMLVKSSVFPSPI--------QREVLNESKKKLLAGP 180
                             + + PE    +S + S          Q    N ++K L  G 
Sbjct: 121 PLPKNVARKSLDGGKSVDSVILPERKKTTSTWSSTSRLSETKSRQSMTKNGARKSLEGGS 180

Query: 181 R----PFPESRS--------------------------------QSPIVLRPMQKEVLNE 240
                 F E ++                                +SPI   P+Q+E+ N+
Sbjct: 181 SVDCVVFSERKNTTSTTRAKSELSSTSRLHEVDSTTFSKPKLPVESPIFPTPVQREIPNK 240

Query: 241 SKKKLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLK 300
            KKKLL                SSP QREVLN  KKKLL EPR    SRS T N+LKNLK
Sbjct: 241 RKKKLL----------------SSPKQREVLNERKKKLLVEPRTLPTSRSSTKNSLKNLK 300

Query: 301 PRASSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSE 360
           P A +ATR  ED+VVQV  K+K R LPEK D I K KSIKVKPLRSAGS +N R+K+ S+
Sbjct: 301 PEAFTATRRQEDSVVQVFAKAKGRELPEKSDKILKPKSIKVKPLRSAGSLDNSRKKSYSK 360

Query: 361 VGKSLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKR-SNLKAVPLKTRSKTKIAERE 420
           +GK L TSK AAK+V A++  S SSNSI GAAN T RK   NLK VPLK R+  K +ER 
Sbjct: 361 MGKCLETSKGAAKKVVAASTGSYSSNSIHGAANLTARKHVGNLKGVPLKNRNMIKKSERG 420

Query: 421 QVQSEEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVP 480
           QVQSEEVQEKT QS           +E+QEKTLYVIKIENE+   Q DQ+ET+DNMEAV 
Sbjct: 421 QVQSEEVQEKTLQS-----------KEIQEKTLYVIKIENEKIPQQPDQDETNDNMEAVS 480

Query: 481 FSPPKSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHN--VSSEGGKE 540
             PP+SLS P +P+ L N ED DVSEYTESEAE+D Y E DE+GS E  +   SSEG + 
Sbjct: 481 SLPPESLSPPISPALLPNVEDQDVSEYTESEAENDFYYEGDEIGSSEPEDNITSSEGSEN 540

Query: 541 VGSHNLGMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFR 600
             S N G+  S+ KDP+STKLSFRRG++IDI SESNSPRRLKFRRGR LGENQ+A DG R
Sbjct: 541 GRSRNHGILPSKEKDPRSTKLSFRRGRVIDIHSESNSPRRLKFRRGRLLGENQKAGDGLR 600

Query: 601 KNFKKVKEVDSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVK 616
           KNFK+ KEVDS+TNT  TA ETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVK
Sbjct: 601 KNFKRGKEVDSETNT--TAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVK 650

BLAST of MS003260 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 165.6 bits (418), Expect = 1.2e-40
Identity = 197/605 (32.56%), Postives = 280/605 (46.28%), Query Frame = 0

Query: 10  VTPETIVAAGTNLR-RSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGRKHAFETKT 69
           VTPE ++     L       G+  +S   EK +PHYLRASTGSCHD CKYG++     K 
Sbjct: 10  VTPENMMNKEDGLNPGGDSTGKAMTSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKP 69

Query: 70  RLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDSTTFVRPEMLV 129
                K +  KSLD         L E  K  S++   K     +   TD +  V      
Sbjct: 70  WRSSTKKIFKKSLDDN-------LNETLKPGSSKMKKKVREVEKNEGTDDSFEV------ 129

Query: 130 KSSVFPSPIQREVLNESKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKKKLLVEPRP 189
                   I+REV+      + +G R                + EVL             
Sbjct: 130 --------IKREVVKYQASGVSSGMR----------------KPEVL------------- 189

Query: 190 LPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPE 249
           +  S   TPV    ++++   +SK K    P P L SRS  +  +  LKP+         
Sbjct: 190 IISSCDETPV--KQIKKKTTLSSKLK----PSPDLGSRS--SENVDALKPK--------- 249

Query: 250 DAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGKSLVTSKEA 309
                VL+KS    L      ++  K +    L+      N  +  D +V K  V+S+ A
Sbjct: 250 -----VLKKSYS-ALTTSKPKVNHEKVVASPVLKPKMGKRNDGKDEDGKVKKGTVSSRVA 309

Query: 310 AKRVAASARASLSSN-SIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQSEEVQEKT 369
           +K+   + RASLS   S+R A + ++RK  +LKA    +R         Q Q      +T
Sbjct: 310 SKKAPVTPRASLSPRLSVRLAGSSSLRKSQSLKAASSSSR---------QNQKPRPVNRT 369

Query: 370 FQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAV--PFSPPKSLSS 429
            +  +++ +       V+EKTL+V+++E          +E D N +    PF PP     
Sbjct: 370 DEFNKQLDD-----YPVEEKTLHVVEMETTNNV----VSENDQNQQGFVEPFLPP----L 429

Query: 430 PPTPSFLANEE--DHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNLGMY 489
           PPT S   ++E    +  EY  +   +++ SE++E+G            KE         
Sbjct: 430 PPTQSTPKDDECTVSETEEYEYTSGSNEAESEEEEIGLSNGEKKPRAARKE--------- 489

Query: 490 QSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEV 549
             +  D  + KL FRRG I+D  +     R+LKFRRGR LGE++      R++FKK +++
Sbjct: 490 -GDSADEAARKLRFRRGTIVDPDTVGEKARKLKFRRGRGLGEDKAQDAQVRRSFKKREDI 506

Query: 550 DSDTNTIETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETV 609
             +   +    E VVLRHQDVQ +KDAQGLFNNVIEETASKLVE RKSKVKALVGAFETV
Sbjct: 550 REE--EVNEDGEKVVLRHQDVQ-EKDAQGLFNNVIEETASKLVEARKSKVKALVGAFETV 506

BLAST of MS003260 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 121.7 bits (304), Expect = 2.0e-27
Identity = 184/617 (29.82%), Postives = 272/617 (44.08%), Query Frame = 0

Query: 1   MAEKGDVLPVTPETIVAAGTNLRRSSMGGQTSSSNSGEKLVPHYLRASTGSCHDFCKYGR 60
           MAE+   LP +P+  +    + RR S  G+ S   + EK+VP+YLR+ TGSCHD CKYGR
Sbjct: 1   MAEETVRLPNSPDVNI----SWRRIST-GKLSFLYTQEKVVPNYLRSPTGSCHDACKYGR 60

Query: 61  KHAFETKTRLPMLKNVASKSLDSESSVDSVVLPERKKTTSTQAHAKFSSASRLSDTDSTT 120
           K   E K R+P  K V S+S     ++DS   P RKK  +        S SR  D+    
Sbjct: 61  KDESEDKPRVPHRKRV-SRSFSGAINLDS---PLRKKALT----KPLLSPSRRCDSVG-G 120

Query: 121 FVRPEMLVKSSVFPSPIQREVLNESKKKLLAGPRPFPESRSQSPIVLRPMQKEVLNESKK 180
           F   +  V++  F S +      + KK    G      S S+S          + + +K+
Sbjct: 121 FDHAKSQVRN--FSSGV-----CDVKKSHADGTNEKVVSVSES---------RLADSTKR 180

Query: 181 KLLVEPRPLPKSRSRTPVFSSPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRA 240
           K         K + +T   S    +E++   ++                  ALK LK   
Sbjct: 181 K---------KKKKKTVYVSRGRAKEIVEQKRR----------------VTALK-LK--- 240

Query: 241 SSATRNPEDAVVQVLEKSKERRLPEKHDDISKSKSIKVKPLRSAGSPENLRRKNDSEVGK 300
                    AV Q  E +  R             ++K K +                   
Sbjct: 241 ---------AVAQTAEIALRR------------STVKRKKMNGGSK-------------- 300

Query: 301 SLVTSKEAAKRVAASARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQS 360
               + E  K V A  RAS+SS     +     +K SN  +VPLK               
Sbjct: 301 ----AAEQKKAVMALRRASMSSKGC--SRCLKTKKESNSLSVPLK--------------- 360

Query: 361 EEVQEKTFQSEEEIQEKTFQIEEVQEKTLYVIKIENEEKSPQYDQNETDDNMEAVPFSPP 420
                   ++ + + +K   +  V+EKTLYVIK+E  ++  + + N+    +   P   P
Sbjct: 361 --------KTRKHVGDKCKDL--VEEKTLYVIKMETVDEIVESELNQR--CVMDSPIDDP 420

Query: 421 KSLSSPPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNL 480
           KS  S      +  E + +      S+ E+D   ED+ +   E  N + EG  +  S   
Sbjct: 421 KSEKSQDKGECIETEHEDE-----SSQEEEDEEEEDENVSVSEDKNTTREGKSKAFSAES 478

Query: 481 GMYQSEGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQL-GENQRAVDGFRKNFKK 540
            +         + KL  RRGKIID  SE NSPR+LKF+RG+ + G +  +  G R+   K
Sbjct: 481 AI------TGNAMKLRIRRGKIIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRL-K 478

Query: 541 VKEVDSDTNTIETAPETVVLRHQDVQGKKDAQG-LFNNVIEETASKLVETRKSKVKALVG 600
            K  +   +  +     VVL+HQD + K++++  LFN VI+ETA+KLV+TRKSKVKALVG
Sbjct: 541 TKGTNLSNDKEQQRKPRVVLKHQDTEKKRESRVLLFNKVIKETANKLVQTRKSKVKALVG 478

Query: 601 AFETVISLQDGKPSLDT 616
           AFE+VISLQ+   S  T
Sbjct: 601 AFESVISLQEKTSSATT 478

BLAST of MS003260 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-14
Identity = 100/344 (29.07%), Postives = 165/344 (47.97%), Query Frame = 0

Query: 275 SIKVKPLRSAGSPENLRRKNDSEVGKSL----VTSKEAAKRVAASARASLSSNSIRGAAN 334
           ++K KP  S  S  +   K  SE+ KS+    V S + A++   +      S  ++    
Sbjct: 165 AVKKKPCASVNSESS--SKEGSEIAKSVDGLSVKSNDRARKNKETESGLSGSAVVKKVPA 224

Query: 335 FTVRKRSNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSEEEIQEKTFQIEEVQEKTLY 394
               K S    V       +K+   + +++ E  + T         +T   E+V+EKT+ 
Sbjct: 225 LRTDKSSTSTGV-----GSSKVCAPKNLKNVEKAKTT---------QTISGEDVKEKTVC 284

Query: 395 V----IKIENEEKSPQYDQNETDDNMEAVPFSPPKSLSSPPTPSFLANEEDHDVSEYTES 454
           V    +K    EK P  ++       +++  +P +   S PT                  
Sbjct: 285 VVESSVKGVKSEKQPSSEKKTMKSGNKSLSTTPKR--GSSPTKQIPGK------------ 344

Query: 455 EAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNLGMYQSEGKDPQSTKLSFRRGKIIDIR 514
                  ++  E GS +    + +  K+V     G+     K   + +++F++GK++D +
Sbjct: 345 --ISTGLTKKKETGSADVVEANPKPEKKVRPKKTGV-----KVSLAQQMTFKKGKVLDPK 404

Query: 515 SESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVK-EVDSDTNTIE-TAPETVVLRHQDV 574
            E +SPR +KF++ R + E +   +G +KN K  +  V++ T++ E +  E VVLRH+ V
Sbjct: 405 PEDSSPRWIKFKK-RVVQELKTQSEGKKKNLKDRRLGVETKTDSCEGSKREKVVLRHRKV 464

Query: 575 QGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQD 609
           +GKK    LFNNVIEET +KL + RK KVKAL+GAFETVISLQD
Sbjct: 465 EGKKKMITLFNNVIEETVNKLTKVRKHKVKALIGAFETVISLQD 470

BLAST of MS003260 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 74.7 bits (182), Expect = 2.9e-13
Identity = 116/425 (27.29%), Postives = 195/425 (45.88%), Query Frame = 0

Query: 201 SPMQREVLNASKKKLLAEPRPSLKSRSHTTNALKNLKPRASSATRNPEDAVVQVLEKSKE 260
           S   +  L A+K++   E   S        +  K+  P  S   R  +   + V + S+ 
Sbjct: 128 SKPDKSSLQAAKREAAGEVVKSCDGLHVKKSETKSTSPSVSPVVRTVKKTNLVVNKASRI 187

Query: 261 RRLPEKHDDISKSKSIK-----VKPLRSAGSPENLRRKNDSEVGKSLVTSKEAAKRVAAS 320
            +     +D+SK+   K     V+P+R     +++  K D EV K    S+        S
Sbjct: 188 SQNKSPKEDLSKNLKNKEKGKIVEPVRC----DDVLEKTDLEVKKVSRISEN------KS 247

Query: 321 ARASLSSNSIRGAANFTVRKRSNLKAVPLKTRSKTKIAEREQVQSEEVQEKTFQSEEEIQ 380
           ++     N  +   +  VR    L+   L  +  ++I+E +  + E ++    + +  I 
Sbjct: 248 SKEDTLKNKEKAKIDEPVRCDDVLEKTSLDAQKVSRISENKNSKEERLKNLKNKEKTNID 307

Query: 381 EKTFQIEEVQEKTLYVIKIENEE-------KSPQYDQNETDDNMEAVPFSPPKSLS---S 440
           E   + ++  EKTLYV++   E+       KS +  + +     + +  +  KSLS   S
Sbjct: 308 EPV-RPDDAVEKTLYVVESSVEKKKKKMSTKSVKISETQQSSEKKIIRSTGKKSLSLLPS 367

Query: 441 PPTPSFLANEEDHDVSEYTESEAEDDSYSEDDELGSMEAHNVSSEGGKEVGSHNLGMYQS 500
            P PS +    D      T S ++  S  E  + GS      + +   ++    +G+  +
Sbjct: 368 LPPPSEVVTGSDPRPIRQTTSRSK-TSLPEKKQSGSANL-VTNPKPESKIRPKRIGLKVT 427

Query: 501 EGKDPQSTKLSFRRGKIIDIRSESNSPRRLKFRRGRQLGENQRAVDGFRKNFKKVKEVDS 560
               P   +++F++GK+++ + E ++   +KF++   + E +       K  K +K+   
Sbjct: 428 PPPPPTKQQMNFKKGKVLEPKPEDSTTTSIKFKK-IVVQEPKLRTSDVNKKKKSLKDKRE 487

Query: 561 DTNTI--ETAPETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETV 609
               I  E   E VVLRH+ V+ KK  Q LFNNVIEET +KL E RKSKVKALVGAFETV
Sbjct: 488 GVGKINGEGKREKVVLRHRKVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKALVGAFETV 538

BLAST of MS003260 vs. TAIR 10
Match: AT3G54570.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 46.6 bits (109), Expect = 8.4e-05
Identity = 26/57 (45.61%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 549 TIETAPETVVLRHQDVQGKKDA-QGLFNNVIEETASKLVETRKSKVKALVGAFETVI 605
           T+E + ETV LRHQD+  +K A + + +  ++ T SKLV  RK  V  LV AFET +
Sbjct: 359 TVEPSNETVDLRHQDMDERKKAEEWMIDYALQHTVSKLVVERKKDVALLVEAFETTV 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134206.10.0e+0099.19uncharacterized protein LOC111006524 [Momordica charantia] >XP_022134207.1 uncha... [more]
XP_004141582.31.8e-17457.12uncharacterized protein LOC101213671 isoform X3 [Cucumis sativus] >XP_031741331.... [more]
XP_031741329.11.8e-17457.12uncharacterized protein LOC101213671 isoform X1 [Cucumis sativus][more]
XP_031741330.11.8e-17457.12uncharacterized protein LOC101213671 isoform X2 [Cucumis sativus][more]
XP_008459364.18.6e-16959.41PREDICTED: uncharacterized protein LOC103498520 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C1B90.0e+0099.19uncharacterized protein LOC111006524 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A1S3C9Z24.1e-16959.41uncharacterized protein LOC103498520 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3CA315.4e-16958.93uncharacterized protein LOC103498520 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3BNG06.0e-16858.35Plant calmodulin-binding protein-related, putative isoform 1 OS=Cucumis melo var... [more]
A0A0A0KSH74.0e-16458.00CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G638370... [more]
Match NameE-valueIdentityDescription
AT5G39380.11.2e-4032.56Plant calmodulin-binding protein-related [more]
AT5G15430.12.0e-2729.82Plant calmodulin-binding protein-related [more]
AT5G61260.11.5e-1429.07Plant calmodulin-binding protein-related [more]
AT5G07820.12.9e-1327.29Plant calmodulin-binding protein-related [more]
AT3G54570.18.4e-0545.61Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 491..605
e-value: 1.1E-34
score: 131.2
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 494..605
e-value: 1.9E-36
score: 125.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..501
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..372
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 286..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 223..248
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 440..457
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..302
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 2..612
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 2..612

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003260.1MS003260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding