MS003185 (gene) Bitter gourd (TR) v1

Overview


Name	MS003185
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phytolongin Phyl2.2-like
Location	scaffold234: 1004221 .. 1004994 (-)
RNA-Seq Expression	MS003185
Synteny	MS003185

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTCGAATCCAGATCTCGTCTTCTATGCCTGCATTGCCAGAGGAACCGTAATCCTCGCGGAATTTACGAGAGAATCAAATCTCGATGACTTAGCTTTCAGATGCATCGAGAAAGCCCCTCCTAATCACTCCACCTTCTCCCACACCGTCCGAAACAGAGCGTACGCCTTTTTGATTGAAGAATCCTTCGTTTATTTCGGAATCTTCCATGAAGACCTCTACAAATCGGAGGTTTCTGGTTTCTTAAACCGTGTAAAGCGTGTTTTCACGGACTTCATCGAGAGCGAATTGCTCAATGGTTTCGACAATTTCACCCCCTATTGCTTTCAGGCGCAGTTCGATTCTATCTTCCGGCAGATTCTAGGGTCCAATCTGAACACGTCGGGGAATTTATCGACGTCGTCCGGCGTATTGAGGCCGAGTGTGGATTCAAGTATAAGAAAGCAGATACCGTTCATATCGAAGTTCTCAAATCCCAGTAACTTGATGAAGAAGAAGAAGAAGGTGGTTGGAGATGCTAATGGCGAAGGAGGAAAGTATGCAACAACCATGGATGATTCTGCGGATCCTTATGATGACATGAATGGTTTAGGAAGTAGAGATATCACATTGCTGATGCAAAAGAACGGTTCACATATTGCCGAGCGTCAGAAAGCAAAGCAAATATGGCAGAAACATGTGTGGGTTGTTTTGTCCATCGATTTGTTTGTGTGTCTGGTTTTGTTCGCCATATGGCTGTGGGTTTGCAGGGGATTTCAATGCATTGAGAGC

mRNA sequence

ATGGTTTCGAATCCAGATCTCGTCTTCTATGCCTGCATTGCCAGAGGAACCGTAATCCTCGCGGAATTTACGAGAGAATCAAATCTCGATGACTTAGCTTTCAGATGCATCGAGAAAGCCCCTCCTAATCACTCCACCTTCTCCCACACCGTCCGAAACAGAGCGTACGCCTTTTTGATTGAAGAATCCTTCGTTTATTTCGGAATCTTCCATGAAGACCTCTACAAATCGGAGGTTTCTGGTTTCTTAAACCGTGTAAAGCGTGTTTTCACGGACTTCATCGAGAGCGAATTGCTCAATGGTTTCGACAATTTCACCCCCTATTGCTTTCAGGCGCAGTTCGATTCTATCTTCCGGCAGATTCTAGGGTCCAATCTGAACACGTCGGGGAATTTATCGACGTCGTCCGGCGTATTGAGGCCGAGTGTGGATTCAAGTATAAGAAAGCAGATACCGTTCATATCGAAGTTCTCAAATCCCAGTAACTTGATGAAGAAGAAGAAGAAGGTGGTTGGAGATGCTAATGGCGAAGGAGGAAAGTATGCAACAACCATGGATGATTCTGCGGATCCTTATGATGACATGAATGGTTTAGGAAGTAGAGATATCACATTGCTGATGCAAAAGAACGGTTCACATATTGCCGAGCGTCAGAAAGCAAAGCAAATATGGCAGAAACATGTGTGGGTTGTTTTGTCCATCGATTTGTTTGTGTGTCTGGTTTTGTTCGCCATATGGCTGTGGGTTTGCAGGGGATTTCAATGCATTGAGAGC

Coding sequence (CDS)

ATGGTTTCGAATCCAGATCTCGTCTTCTATGCCTGCATTGCCAGAGGAACCGTAATCCTCGCGGAATTTACGAGAGAATCAAATCTCGATGACTTAGCTTTCAGATGCATCGAGAAAGCCCCTCCTAATCACTCCACCTTCTCCCACACCGTCCGAAACAGAGCGTACGCCTTTTTGATTGAAGAATCCTTCGTTTATTTCGGAATCTTCCATGAAGACCTCTACAAATCGGAGGTTTCTGGTTTCTTAAACCGTGTAAAGCGTGTTTTCACGGACTTCATCGAGAGCGAATTGCTCAATGGTTTCGACAATTTCACCCCCTATTGCTTTCAGGCGCAGTTCGATTCTATCTTCCGGCAGATTCTAGGGTCCAATCTGAACACGTCGGGGAATTTATCGACGTCGTCCGGCGTATTGAGGCCGAGTGTGGATTCAAGTATAAGAAAGCAGATACCGTTCATATCGAAGTTCTCAAATCCCAGTAACTTGATGAAGAAGAAGAAGAAGGTGGTTGGAGATGCTAATGGCGAAGGAGGAAAGTATGCAACAACCATGGATGATTCTGCGGATCCTTATGATGACATGAATGGTTTAGGAAGTAGAGATATCACATTGCTGATGCAAAAGAACGGTTCACATATTGCCGAGCGTCAGAAAGCAAAGCAAATATGGCAGAAACATGTGTGGGTTGTTTTGTCCATCGATTTGTTTGTGTGTCTGGTTTTGTTCGCCATATGGCTGTGGGTTTGCAGGGGATTTCAATGCATTGAGAGC

Protein sequence

MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLIEESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGKYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCLVLFAIWLWVCRGFQCIES

Homology

[Close]

BLAST of MS003185 vs. NCBI nr
Match: XP_022133479.1 (phytolongin Phyl2.2-like [Momordica charantia])

HSP 1 Score: 521.2 bits (1341), Expect = 5.2e-144
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ
Sbjct: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK
Sbjct: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL
Sbjct: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLFAIWLWVCRGFQCIES
Sbjct: 241 VLFAIWLWVCRGFQCIES 258

[Close]

BLAST of MS003185 vs. NCBI nr
Match: XP_038890434.1 (phytolongin Phyl2.1-like [Benincasa hispida])

HSP 1 Score: 407.5 bits (1046), Expect = 8.4e-110
Identity = 205/259 (79.15%), Postives = 223/259 (86.10%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGT+ILAEFTRESNLDDLAFRCIEKAPP+HS FSHTVRNRAY FLI
Sbjct: 1   MVSNPDLVFYACIARGTIILAEFTRESNLDDLAFRCIEKAPPHHSIFSHTVRNRAYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E  FVYFGIFHEDL KSE+ GFLNR +RVF +F+ESE L GF+NFT YCFQ+QFDS  R 
Sbjct: 61  EGPFVYFGIFHEDLNKSEICGFLNRSRRVFEEFLESESLKGFENFTSYCFQSQFDSTLRY 120

Query: 121 -ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGG 180
            +LGSNLN + + +   G  R S+DSS RKQIP ISKFSNP +LMKKKKKV+GD N EGG
Sbjct: 121 LVLGSNLNKTSS-NALKGTQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVIGDVNSEGG 180

Query: 181 KYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVC 240
           KYA  MDDS D YDD NGL SRDITLLMQKNGSH+ ERQKAKQ+W+KHVWVVLSIDLFVC
Sbjct: 181 KYA--MDDSVDLYDDNNGLCSRDITLLMQKNGSHLIERQKAKQVWRKHVWVVLSIDLFVC 240

Query: 241 LVLFAIWLWVCRGFQCIES 259
           LVLFAIWLWVCRGFQCIES
Sbjct: 241 LVLFAIWLWVCRGFQCIES 256

[Close]

BLAST of MS003185 vs. NCBI nr
Match: XP_008458710.1 (PREDICTED: phytolongin Phyl2.1-like [Cucumis melo])

HSP 1 Score: 398.3 bits (1022), Expect = 5.1e-107
Identity = 200/258 (77.52%), Postives = 218/258 (84.50%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFY+CIARGT+ILAEFTRE NLDDL  RCIEKAPP+HS FSHT+RNRAY FLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E SFVYFGIFHEDL KSEVSGFLNR+KR F  F+ESE L GF+NFT YCFQ+QFDS FR 
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           +LG NLN + + +   G  R S+DSS RKQIP +SKFSNP +LMKKKKKV GD N EGGK
Sbjct: 121 LLGLNLNKTSS-NGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           Y   MDDS D YDD NGL SRDITLLMQKNGSH +ERQKAKQ+W+KHVWVVLSIDLFVCL
Sbjct: 181 YG--MDDSVDLYDDNNGLCSRDITLLMQKNGSHNSERQKAKQVWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLF IWLWVCRGFQCIES
Sbjct: 241 VLFGIWLWVCRGFQCIES 255

[Close]

BLAST of MS003185 vs. NCBI nr
Match: XP_004148677.1 (phytolongin Phyl2.1 [Cucumis sativus])

HSP 1 Score: 391.3 bits (1004), Expect = 6.2e-105
Identity = 195/258 (75.58%), Postives = 217/258 (84.11%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLV Y+CIARGT+ILAEFTRE NLDDL FRCIEK PP+HS FSHT+RNRAY FLI
Sbjct: 1   MVSNPDLVLYSCIARGTIILAEFTREPNLDDLPFRCIEKTPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E SFVYFGIFHEDL KSEVSGFLNR+KR F  F+ESE L GF+NFT +CFQ+ FDS FR 
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSHCFQSHFDSSFRH 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           +LG NLN + + +   G  R S+DSS+RKQIP +SKFSNP +LMKKKKKV GD N +GGK
Sbjct: 121 LLGLNLNKTSS-NGLKGSQRSSLDSSLRKQIPLVSKFSNPGSLMKKKKKVTGDGNWDGGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           Y   MDDS D YDD NGL SRDITLLMQK+GSH +ERQKAKQ+W+KHVWVVLSIDLFVCL
Sbjct: 181 YG--MDDSVDLYDDNNGLCSRDITLLMQKHGSHNSERQKAKQVWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLF IWLWVCRGFQCIES
Sbjct: 241 VLFGIWLWVCRGFQCIES 255

[Close]

BLAST of MS003185 vs. NCBI nr
Match: XP_022963950.1 (phytolongin Phyl2.2-like [Cucurbita moschata] >KAG6602468.1 Phytolongin 2.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033142.1 Phytolongin Phyl2.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 385.2 bits (988), Expect = 4.5e-103
Identity = 199/258 (77.13%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGTVILAEF RE NL+DLAFRCIEKAPP+HSTFSHTVRNR Y FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFNREPNLNDLAFRCIEKAPPHHSTFSHTVRNRVYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E  FVYFGIFHEDL++SEV GFLNR++R F DFIESEL  GF+NFT  CFQ+QFDSIFR+
Sbjct: 61  EGPFVYFGIFHEDLFRSEVCGFLNRLRRAFEDFIESELRKGFENFTSNCFQSQFDSIFRR 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           IL SN +      TSSG     +DS  RK+   ISKFS P +LMKKKKKVVGDAN E GK
Sbjct: 121 ILTSNQD-----QTSSG-----LDSDKRKKTSLISKFSKPCSLMKKKKKVVGDANSEEGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           YA+T+DDS D YDD NGL SRDITLLMQKNGS+  ERQKAKQIW+KHVWVVLSIDLFVCL
Sbjct: 181 YASTVDDSVDLYDDNNGLCSRDITLLMQKNGSY--ERQKAKQIWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLFAIWLWVC GFQCIES
Sbjct: 241 VLFAIWLWVCSGFQCIES 246

[Close]

BLAST of MS003185 vs. ExPASy Swiss-Prot
Match: Q9LVV3 (Phytolongin Phyl2.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.2 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 4.4e-45
Identity = 107/274 (39.05%), Postives = 154/274 (56.20%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFT--RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAF 60
           M+SNP L+ Y CIA+GTV+LAEF   +E  ++ +A RCIE  PP+HS FSHTV  + Y F
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFD-----NFTPYCFQA 120
            I ++SFVYF I  E + K E    LNR++    D I+     G D     N   +C Q 
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKD---GGSDVETLINPVSHCLQL 120

Query: 121 QFDSIFRQILG------SNLNTSGN-LSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMK 180
           + D +F +I+G       +++  G+  S +     PS+DSS  ++   +     P   +K
Sbjct: 121 KLDPVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALK 180

Query: 181 KKKKVVGDANGEGGKYATTMDDSADPYDDMNGLGSRDITLLMQKNG--SHIAERQKAKQI 240
           KKK++  +A G       ++ + ++   D+ G G+  +     +NG  S    RQKAKQI
Sbjct: 181 KKKRLHNEAKGGDSCEVGSIQEISEKNVDLCGNGNNGVLRKELRNGLLSDHHHRQKAKQI 240

Query: 241 WQKHVWVVLSIDLFVCLVLFAIWLWVCRGFQCIE 258
           W+KHVWVVL  D  +C VLF IWLW+C GFQCI+
Sbjct: 241 WKKHVWVVLMFDFCICAVLFGIWLWICEGFQCIQ 271

[Close]

BLAST of MS003185 vs. ExPASy Swiss-Prot
Match: Q9STP2 (Phytolongin Phyl2.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.1 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.9e-43
Identity = 110/271 (40.59%), Postives = 144/271 (53.14%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFT--RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAF 60
           M SN  ++FY+CIA+GTVILAEF    E  ++DLA RCIE  PP+HS  SHTV  R YA 
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIE-SELLNGFDNFTPYCFQAQFDSI 120
           +I+  F YF I  E + KSE     NR+K      +E     +  DN T +C Q++ D +
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRQILGSNLNTSGNLSTSSGVLR----------PSVDSSI-RKQIPFISKFSNPSNLMKK 180
           F +I     N + +L    G  R           S+DSS  RK    +     P  ++K 
Sbjct: 121 FAEIAAIGGNHNKDLELEFGSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLKN 180

Query: 181 KKKVVGDANGEGGKYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQK 240
           KK++  +A  EG      MD         NGL           +  H   RQKAKQIW+K
Sbjct: 181 KKRLQTEAKSEGHWNEKKMDLGGGGKGVRNGL----------IHDHH--HRQKAKQIWRK 240

Query: 241 HVWVVLSIDLFVCLVLFAIWLWVCRGFQCIE 258
           HVW+VL  DL +CLVLF IWLW+C+GFQCI+
Sbjct: 241 HVWIVLMFDLCICLVLFGIWLWICQGFQCID 259

[Close]

BLAST of MS003185 vs. ExPASy Swiss-Prot
Match: Q9SN26 (Phytolongin Phyl1.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.1 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.1e-17
Identity = 73/259 (28.19%), Postives = 122/259 (47.10%), Query Frame = 0


Query: 8   VFYACIARGTVILAEFT-RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLIEESFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+  R + FLI + FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYC----FQAQFDSIFRQIL 127
           F I  E L +S V  FL  ++    +F ++   N   +FT        + Q   +  +++
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLR---DEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLI 127

Query: 128 GS---NLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGG 187
            S      +S N    S  L    + S   + P + + S      K++KK   D   E  
Sbjct: 128 ASLERVAESSSNNELKSSNLGEQSEGSNSTKAPLLGRLS------KQEKKKGKDHVIELE 187

Query: 188 KYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVC 247
           ++  + +D  +  DD  G G+      +  +G  + +  + K  W++ V +VL+ID  +C
Sbjct: 188 EHRKS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWK--WRRLVQIVLAIDAAIC 247

Query: 248 LVLFAIWLWVCRGFQCIES 259
           L LF IWL +CRG +C  S
Sbjct: 248 LTLFGIWLAICRGIECTRS 254

[Close]

BLAST of MS003185 vs. ExPASy Swiss-Prot
Match: Q84WF5 (Phytolongin Phyl1.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.2 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-13
Identity = 70/269 (26.02%), Postives = 118/269 (43.87%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTR---ESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYA 60
           M S  + V Y C++R   I+  +       N + LA  C+EK PP H  +  T   + + 
Sbjct: 1   MGSIQNTVHYCCVSRDNQIMYAYNNAGDHRNNESLAALCLEKTPPFHKWYFETRGKKTFG 60

Query: 61  FLIEESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFT-PYCFQAQFDS 120
           FL+++ FVYF I  +   KS V  FL +++    +  E+   N   +F+    F    D 
Sbjct: 61  FLMKDDFVYFAIVDDVFKKSSVLDFLEKLR---DELKEANKKNSRGSFSGSISFSNVQDQ 120

Query: 121 IFRQILGSNLNTSGNLSTSSGVLRPSVDS-----SIRKQIPFISKFSNPSNLMKKKKKVV 180
           I R+++ S       L  SS    PS+D      +   + P + + +      + KKK  
Sbjct: 121 IVRRLIASLEFDHTCLPLSS----PSIDGAEQSYASNSKAPLLGRSNK-----QDKKKGR 180

Query: 181 GDANGEGGKYATTMDDSAD--PYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVW 240
             A+   G        S D     + +   S   T + ++  S     Q  ++ W++ V 
Sbjct: 181 DHAHSLRGIEIEEHRKSNDRGNVTECSNASSESATYVPRRGRS--GGSQSIERKWRRQVK 240

Query: 241 VVLSIDLFVCLVLFAIWLWVCRGFQCIES 259
           +VL+ID+ +CL L  +WL +C G +C  S
Sbjct: 241 IVLAIDIAICLTLLGVWLAICHGIECTRS 255

[Close]

BLAST of MS003185 vs. ExPASy Swiss-Prot
Match: Q9M376 (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP727 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 9.3e-11
Identity = 59/249 (23.69%), Postives = 110/249 (44.18%), Query Frame = 0


Query: 8   VFYACIARGTVILAEFTRES-NLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLIEESFVY 67
           + Y+ +A+GTV+LAE T  S N   +A +C++K P N S ++++     + FL++  FV+
Sbjct: 6   LIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVDNGFVF 65

Query: 68  FGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQILGSNL 127
             +  E   +S    FL RVK  F    E+ + N  D   P   + + D +F    G   
Sbjct: 66  LVVADESTGRSVPFVFLERVKEDFKKRYEASIKN--DERHPLADEDEDDDLF----GDRF 125

Query: 128 NTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGKYATTMD 187
           + + NL    G +       +++ + +    S+P   M K  K+        G     ++
Sbjct: 126 SVAYNLDREFGPI-------LKEHMQYC--MSHPEE-MSKLSKLKAQITEVKGIMMDNIE 185

Query: 188 DSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCLVLFAIW 247
              D  + +  L  +   L  Q +      RQ  +++W + + + L +   V   +  +W
Sbjct: 186 KVLDRGEKIELLVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGAVFSFILIVW 238

Query: 248 LWVCRGFQC 256
           +  C GF+C
Sbjct: 246 VVACGGFKC 238

[Close]

BLAST of MS003185 vs. ExPASy TrEMBL
Match: A0A6J1BW35 (phytolongin Phyl2.2-like OS=Momordica charantia OX=3673 GN=LOC111006049 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.5e-144
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ
Sbjct: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK
Sbjct: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL
Sbjct: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLFAIWLWVCRGFQCIES
Sbjct: 241 VLFAIWLWVCRGFQCIES 258

[Close]

BLAST of MS003185 vs. ExPASy TrEMBL
Match: A0A1S3C809 (phytolongin Phyl2.1-like OS=Cucumis melo OX=3656 GN=LOC103498036 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.5e-107
Identity = 200/258 (77.52%), Postives = 218/258 (84.50%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFY+CIARGT+ILAEFTRE NLDDL  RCIEKAPP+HS FSHT+RNRAY FLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E SFVYFGIFHEDL KSEVSGFLNR+KR F  F+ESE L GF+NFT YCFQ+QFDS FR 
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           +LG NLN + + +   G  R S+DSS RKQIP +SKFSNP +LMKKKKKV GD N EGGK
Sbjct: 121 LLGLNLNKTSS-NGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           Y   MDDS D YDD NGL SRDITLLMQKNGSH +ERQKAKQ+W+KHVWVVLSIDLFVCL
Sbjct: 181 YG--MDDSVDLYDDNNGLCSRDITLLMQKNGSHNSERQKAKQVWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLF IWLWVCRGFQCIES
Sbjct: 241 VLFGIWLWVCRGFQCIES 255

[Close]

BLAST of MS003185 vs. ExPASy TrEMBL
Match: A0A6J1HJF1 (phytolongin Phyl2.2-like OS=Cucurbita moschata OX=3662 GN=LOC111464094 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.2e-103
Identity = 199/258 (77.13%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGTVILAEF RE NL+DLAFRCIEKAPP+HSTFSHTVRNR Y FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFNREPNLNDLAFRCIEKAPPHHSTFSHTVRNRVYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E  FVYFGIFHEDL++SEV GFLNR++R F DFIESEL  GF+NFT  CFQ+QFDSIFR+
Sbjct: 61  EGPFVYFGIFHEDLFRSEVCGFLNRLRRAFEDFIESELRKGFENFTSNCFQSQFDSIFRR 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           IL SN +      TSSG     +DS  RK+   ISKFS P +LMKKKKKVVGDAN E GK
Sbjct: 121 ILTSNQD-----QTSSG-----LDSDKRKKTSLISKFSKPCSLMKKKKKVVGDANSEEGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           YA+T+DDS D YDD NGL SRDITLLMQKNGS+  ERQKAKQIW+KHVWVVLSIDLFVCL
Sbjct: 181 YASTVDDSVDLYDDNNGLCSRDITLLMQKNGSY--ERQKAKQIWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLFAIWLWVC GFQCIES
Sbjct: 241 VLFAIWLWVCSGFQCIES 246

[Close]

BLAST of MS003185 vs. ExPASy TrEMBL
Match: A0A6J1JQM5 (phytolongin Phyl2.1-like OS=Cucurbita maxima OX=3661 GN=LOC111487464 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.4e-102
Identity = 197/258 (76.36%), Postives = 216/258 (83.72%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLVFYACIARGTVILAEF RE NL+DLAFRCIEKAPP+HSTFSHTVRNR Y FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFNREPNLNDLAFRCIEKAPPHHSTFSHTVRNRVYTFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E  FVYFGIFHEDL+KSEV GFL+R++R F +FIESEL  GF+NFT  CFQ+QFDSIFR+
Sbjct: 61  EGPFVYFGIFHEDLFKSEVCGFLSRLRRAFEEFIESELRKGFENFTSNCFQSQFDSIFRR 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180
           IL SN +      TSSG     +DS  RK+   ISKFS P +LMKKKKKVVGDAN E GK
Sbjct: 121 ILTSNQD-----QTSSG-----LDSDKRKKTSLISKFSKPCSLMKKKKKVVGDANSEEGK 180

Query: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240
           YA+T+DDS D YDD NGL SRD+TLLMQKNGS+  ERQKAKQIW+KHVWVVLSIDLFVCL
Sbjct: 181 YASTVDDSVDLYDDNNGLCSRDVTLLMQKNGSY--ERQKAKQIWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFQCIES 259
           VLFAIWLWVC GFQCIES
Sbjct: 241 VLFAIWLWVCSGFQCIES 246

[Close]

BLAST of MS003185 vs. ExPASy TrEMBL
Match: A0A6J1FEB5 (phytolongin Phyl2.2-like OS=Cucurbita moschata OX=3662 GN=LOC111444745 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 4.5e-101
Identity = 196/259 (75.68%), Postives = 212/259 (81.85%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60
           MVSNPDLV YACIA GTVILAEFTRE NLDDLAFRCIEKAPP+HS FSHTVR R Y FLI
Sbjct: 1   MVSNPDLVLYACIASGTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120
           E  FVYFGIFHEDLYKSE+ GFLNR+  VF +F+ESE   GF+NFT YCFQ+QFDS FR 
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLSCVFEEFLESESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLM-KKKKKVVGDANGEGG 180
           +LG NLN + + S      R S+DSS RKQIP ISKFSNPS+LM KKKKKVVGDAN E G
Sbjct: 121 LLGLNLNNT-SYSVLKEAQRSSLDSSKRKQIPLISKFSNPSSLMKKKKKKVVGDANEEAG 180

Query: 181 KYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVC 240
           KYA  M DSAD  D  NGL SRD +LL QKNGSH+ ERQK KQIW+KHVWVVLSIDLFVC
Sbjct: 181 KYA--MVDSADLNDHNNGLCSRDSSLLKQKNGSHMIERQKVKQIWRKHVWVVLSIDLFVC 240

Query: 241 LVLFAIWLWVCRGFQCIES 259
           LVLFAIWLWVCRGF+CIES
Sbjct: 241 LVLFAIWLWVCRGFRCIES 256

[Close]

BLAST of MS003185 vs. TAIR 10
Match: AT5G52990.1 (SNARE-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 3.1e-46
Identity = 107/274 (39.05%), Postives = 154/274 (56.20%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFT--RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAF 60
           M+SNP L+ Y CIA+GTV+LAEF   +E  ++ +A RCIE  PP+HS FSHTV  + Y F
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFD-----NFTPYCFQA 120
            I ++SFVYF I  E + K E    LNR++    D I+     G D     N   +C Q 
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKD---GGSDVETLINPVSHCLQL 120

Query: 121 QFDSIFRQILG------SNLNTSGN-LSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMK 180
           + D +F +I+G       +++  G+  S +     PS+DSS  ++   +     P   +K
Sbjct: 121 KLDPVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALK 180

Query: 181 KKKKVVGDANGEGGKYATTMDDSADPYDDMNGLGSRDITLLMQKNG--SHIAERQKAKQI 240
           KKK++  +A G       ++ + ++   D+ G G+  +     +NG  S    RQKAKQI
Sbjct: 181 KKKRLHNEAKGGDSCEVGSIQEISEKNVDLCGNGNNGVLRKELRNGLLSDHHHRQKAKQI 240

Query: 241 WQKHVWVVLSIDLFVCLVLFAIWLWVCRGFQCIE 258
           W+KHVWVVL  D  +C VLF IWLW+C GFQCI+
Sbjct: 241 WKKHVWVVLMFDFCICAVLFGIWLWICEGFQCIQ 271

[Close]

BLAST of MS003185 vs. TAIR 10
Match: AT4G27840.1 (SNARE-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.3e-44
Identity = 110/271 (40.59%), Postives = 144/271 (53.14%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFT--RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAF 60
           M SN  ++FY+CIA+GTVILAEF    E  ++DLA RCIE  PP+HS  SHTV  R YA 
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIE-SELLNGFDNFTPYCFQAQFDSI 120
           +I+  F YF I  E + KSE     NR+K      +E     +  DN T +C Q++ D +
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRQILGSNLNTSGNLSTSSGVLR----------PSVDSSI-RKQIPFISKFSNPSNLMKK 180
           F +I     N + +L    G  R           S+DSS  RK    +     P  ++K 
Sbjct: 121 FAEIAAIGGNHNKDLELEFGSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLKN 180

Query: 181 KKKVVGDANGEGGKYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQK 240
           KK++  +A  EG      MD         NGL           +  H   RQKAKQIW+K
Sbjct: 181 KKRLQTEAKSEGHWNEKKMDLGGGGKGVRNGL----------IHDHH--HRQKAKQIWRK 240

Query: 241 HVWVVLSIDLFVCLVLFAIWLWVCRGFQCIE 258
           HVW+VL  DL +CLVLF IWLW+C+GFQCI+
Sbjct: 241 HVWIVLMFDLCICLVLFGIWLWICQGFQCID 259

[Close]

BLAST of MS003185 vs. TAIR 10
Match: AT4G10170.1 (SNARE-like superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-18
Identity = 73/259 (28.19%), Postives = 122/259 (47.10%), Query Frame = 0


Query: 8   VFYACIARGTVILAEFT-RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLIEESFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+  R + FLI + FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYC----FQAQFDSIFRQIL 127
           F I  E L +S V  FL  ++    +F ++   N   +FT        + Q   +  +++
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLR---DEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLI 127

Query: 128 GS---NLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGG 187
            S      +S N    S  L    + S   + P + + S      K++KK   D   E  
Sbjct: 128 ASLERVAESSSNNELKSSNLGEQSEGSNSTKAPLLGRLS------KQEKKKGKDHVIELE 187

Query: 188 KYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVC 247
           ++  + +D  +  DD  G G+      +  +G  + +  + K  W++ V +VL+ID  +C
Sbjct: 188 EHRKS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWK--WRRLVQIVLAIDAAIC 247

Query: 248 LVLFAIWLWVCRGFQCIES 259
           L LF IWL +CRG +C  S
Sbjct: 248 LTLFGIWLAICRGIECTRS 254

[Close]

BLAST of MS003185 vs. TAIR 10
Match: AT4G10170.2 (SNARE-like superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-18
Identity = 73/259 (28.19%), Postives = 122/259 (47.10%), Query Frame = 0


Query: 8   VFYACIARGTVILAEFT-RESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLIEESFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+  R + FLI + FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYC----FQAQFDSIFRQIL 127
           F I  E L +S V  FL  ++    +F ++   N   +FT        + Q   +  +++
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLR---DEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLI 127

Query: 128 GS---NLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGG 187
            S      +S N    S  L    + S   + P + + S      K++KK   D   E  
Sbjct: 128 ASLERVAESSSNNELKSSNLGEQSEGSNSTKAPLLGRLS------KQEKKKGKDHVIELE 187

Query: 188 KYATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVC 247
           ++  + +D  +  DD  G G+      +  +G  + +  + K  W++ V +VL+ID  +C
Sbjct: 188 EHRKS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWK--WRRLVQIVLAIDAAIC 247

Query: 248 LVLFAIWLWVCRGFQCIES 259
           L LF IWL +CRG +C  S
Sbjct: 248 LTLFGIWLAICRGIECTRS 254

[Close]

BLAST of MS003185 vs. TAIR 10
Match: AT1G33475.1 (SNARE-like superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-14
Identity = 70/269 (26.02%), Postives = 118/269 (43.87%), Query Frame = 0


Query: 1   MVSNPDLVFYACIARGTVILAEFTR---ESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYA 60
           M S  + V Y C++R   I+  +       N + LA  C+EK PP H  +  T   + + 
Sbjct: 1   MGSIQNTVHYCCVSRDNQIMYAYNNAGDHRNNESLAALCLEKTPPFHKWYFETRGKKTFG 60

Query: 61  FLIEESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFT-PYCFQAQFDS 120
           FL+++ FVYF I  +   KS V  FL +++    +  E+   N   +F+    F    D 
Sbjct: 61  FLMKDDFVYFAIVDDVFKKSSVLDFLEKLR---DELKEANKKNSRGSFSGSISFSNVQDQ 120

Query: 121 IFRQILGSNLNTSGNLSTSSGVLRPSVDS-----SIRKQIPFISKFSNPSNLMKKKKKVV 180
           I R+++ S       L  SS    PS+D      +   + P + + +      + KKK  
Sbjct: 121 IVRRLIASLEFDHTCLPLSS----PSIDGAEQSYASNSKAPLLGRSNK-----QDKKKGR 180

Query: 181 GDANGEGGKYATTMDDSAD--PYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVW 240
             A+   G        S D     + +   S   T + ++  S     Q  ++ W++ V 
Sbjct: 181 DHAHSLRGIEIEEHRKSNDRGNVTECSNASSESATYVPRRGRS--GGSQSIERKWRRQVK 240

Query: 241 VVLSIDLFVCLVLFAIWLWVCRGFQCIES 259
           +VL+ID+ +CL L  +WL +C G +C  S
Sbjct: 241 IVLAIDIAICLTLLGVWLAICHGIECTRS 255

The following BLAST results are available for this feature:

BLAST of MS003185 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133479.1	5.2e-144	100.00	phytolongin Phyl2.2-like [Momordica charantia]	[more]
XP_038890434.1	8.4e-110	79.15	phytolongin Phyl2.1-like [Benincasa hispida]	[more]
XP_008458710.1	5.1e-107	77.52	PREDICTED: phytolongin Phyl2.1-like [Cucumis melo]	[more]
XP_004148677.1	6.2e-105	75.58	phytolongin Phyl2.1 [Cucumis sativus]	[more]
XP_022963950.1	4.5e-103	77.13	phytolongin Phyl2.2-like [Cucurbita moschata] >KAG6602468.1 Phytolongin 2.1, par...	[more]

BLAST of MS003185 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVV3	4.4e-45	39.05	Phytolongin Phyl2.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.2 PE=2 SV=1	[more]
Q9STP2	1.9e-43	40.59	Phytolongin Phyl2.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.1 PE=2 SV=1	[more]
Q9SN26	5.1e-17	28.19	Phytolongin Phyl1.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.1 PE=2 SV=1	[more]
Q84WF5	1.5e-13	26.02	Phytolongin Phyl1.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.2 PE=2 SV=1	[more]
Q9M376	9.3e-11	23.69	Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP7...	[more]

BLAST of MS003185 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BW35	2.5e-144	100.00	phytolongin Phyl2.2-like OS=Momordica charantia OX=3673 GN=LOC111006049 PE=3 SV=...	[more]
A0A1S3C809	2.5e-107	77.52	phytolongin Phyl2.1-like OS=Cucumis melo OX=3656 GN=LOC103498036 PE=3 SV=1	[more]
A0A6J1HJF1	2.2e-103	77.13	phytolongin Phyl2.2-like OS=Cucurbita moschata OX=3662 GN=LOC111464094 PE=3 SV=1	[more]
A0A6J1JQM5	1.4e-102	76.36	phytolongin Phyl2.1-like OS=Cucurbita maxima OX=3661 GN=LOC111487464 PE=3 SV=1	[more]
A0A6J1FEB5	4.5e-101	75.68	phytolongin Phyl2.2-like OS=Cucurbita moschata OX=3662 GN=LOC111444745 PE=4 SV=1	[more]

BLAST of MS003185 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52990.1	3.1e-46	39.05	SNARE-like superfamily protein	[more]
AT4G27840.1	1.3e-44	40.59	SNARE-like superfamily protein	[more]
AT4G10170.1	3.6e-18	28.19	SNARE-like superfamily protein	[more]
AT4G10170.2	3.6e-18	28.19	SNARE-like superfamily protein	[more]
AT1G33475.1	1.1e-14	26.02	SNARE-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.450.50	Longin domain	coord: 2..128 e-value: 2.6E-25 score: 91.0
None	No IPR available	PANTHER	PTHR47461:SF3	PHYTOLONGIN PHYL2.2	coord: 1..257
IPR044783	Phytolongin	PANTHER	PTHR47461	PHYTOLONGIN PHYL1.2	coord: 1..257
IPR010908	Longin domain	PROSITE	PS50859	LONGIN	coord: 12..118 score: 8.983999
IPR010908	Longin domain	CDD	cd14824	Longin	coord: 9..122 e-value: 4.47988E-8 score: 48.4061
IPR011012	Longin-like domain superfamily	SUPERFAMILY	64356	SNARE-like	coord: 8..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003185.1	MS003185.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane