MS003172 (gene) Bitter gourd (TR) v1

Overview


Name	MS003172
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	metalloendoproteinase 1-like
Location	scaffold234: 872299 .. 873198 (+)
RNA-Seq Expression	MS003172
Synteny	MS003172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTGAGTTCAAACATTCTCTCCAGCTCTCCTTGATCTCTTTCATCCTCATGGCCTCCATCGCAGCTCACGTGACGTCGGCACGTGATCTTCACAATCACAGTCACCACAAATCATCCCATATCATATTCTCTCAAAATCTTTTAGGAACCCGCAAGGGCCACAACATAGAAGGAATCCACGACGTCAAGACTTACCTCAAACGCTATGGCTACTTGACCCATATCGGCAGCCAGCCAGGCGCCTTCGACGATGACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGCGGAATTTTAGACAAGGAAACGTTAAGCCTAATGTTGCAGCCCCGATGCGGGGTTCCAGATATTTTCCACAACGAGACGGAGCATCTCCACATGGGGTCTCACTACGTCTTCTTCCCGGGGAACCTAAAATGGCCAAAGTCCAAATACAACCTGCGGTACAAGTTCATCCACGATTACCCAGAAGGGTTCAAGGATCCAGTGAGGAAGGCTTTGATTCTGTGGTCTGCGTACAGCCATTTCGGGTTCACAGAAGCTGGCAGGGACGAGTTGGATGACATTAAAATAAGCTTCGAGAGGGGCGCGCATGGAGATAGGCATCCGTTCGTGCCTGGAACGGGAGTGTTGGCACATGCGTTTGCGCCAACAGATGGGAGATTTCACTTGAACGTGGACCAGAAGTGGTCGGTGGGGGCTCAAGAGGGTTATTATGATGTGCAGTCGGTGGCACTGCATGAGATTGGGCATCTTCTTGGCCTCGCCCACAGCAAAACCAAAGAAGCCATCATGTGGCCAGTTATACCCACCAATTTTGTCAAGGGCTTGTATTATGACGATATTGCTGGACTCAAGGCTTTATATGGATTTCCTTATTTT

mRNA sequence

GCTGAGTTCAAACATTCTCTCCAGCTCTCCTTGATCTCTTTCATCCTCATGGCCTCCATCGCAGCTCACGTGACGTCGGCACGTGATCTTCACAATCACAGTCACCACAAATCATCCCATATCATATTCTCTCAAAATCTTTTAGGAACCCGCAAGGGCCACAACATAGAAGGAATCCACGACGTCAAGACTTACCTCAAACGCTATGGCTACTTGACCCATATCGGCAGCCAGCCAGGCGCCTTCGACGATGACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGCGGAATTTTAGACAAGGAAACGTTAAGCCTAATGTTGCAGCCCCGATGCGGGGTTCCAGATATTTTCCACAACGAGACGGAGCATCTCCACATGGGGTCTCACTACGTCTTCTTCCCGGGGAACCTAAAATGGCCAAAGTCCAAATACAACCTGCGGTACAAGTTCATCCACGATTACCCAGAAGGGTTCAAGGATCCAGTGAGGAAGGCTTTGATTCTGTGGTCTGCGTACAGCCATTTCGGGTTCACAGAAGCTGGCAGGGACGAGTTGGATGACATTAAAATAAGCTTCGAGAGGGGCGCGCATGGAGATAGGCATCCGTTCGTGCCTGGAACGGGAGTGTTGGCACATGCGTTTGCGCCAACAGATGGGAGATTTCACTTGAACGTGGACCAGAAGTGGTCGGTGGGGGCTCAAGAGGGTTATTATGATGTGCAGTCGGTGGCACTGCATGAGATTGGGCATCTTCTTGGCCTCGCCCACAGCAAAACCAAAGAAGCCATCATGTGGCCAGTTATACCCACCAATTTTGTCAAGGGCTTGTATTATGACGATATTGCTGGACTCAAGGCTTTATATGGATTTCCTTATTTT

Coding sequence (CDS)

GCTGAGTTCAAACATTCTCTCCAGCTCTCCTTGATCTCTTTCATCCTCATGGCCTCCATCGCAGCTCACGTGACGTCGGCACGTGATCTTCACAATCACAGTCACCACAAATCATCCCATATCATATTCTCTCAAAATCTTTTAGGAACCCGCAAGGGCCACAACATAGAAGGAATCCACGACGTCAAGACTTACCTCAAACGCTATGGCTACTTGACCCATATCGGCAGCCAGCCAGGCGCCTTCGACGATGACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGCGGAATTTTAGACAAGGAAACGTTAAGCCTAATGTTGCAGCCCCGATGCGGGGTTCCAGATATTTTCCACAACGAGACGGAGCATCTCCACATGGGGTCTCACTACGTCTTCTTCCCGGGGAACCTAAAATGGCCAAAGTCCAAATACAACCTGCGGTACAAGTTCATCCACGATTACCCAGAAGGGTTCAAGGATCCAGTGAGGAAGGCTTTGATTCTGTGGTCTGCGTACAGCCATTTCGGGTTCACAGAAGCTGGCAGGGACGAGTTGGATGACATTAAAATAAGCTTCGAGAGGGGCGCGCATGGAGATAGGCATCCGTTCGTGCCTGGAACGGGAGTGTTGGCACATGCGTTTGCGCCAACAGATGGGAGATTTCACTTGAACGTGGACCAGAAGTGGTCGGTGGGGGCTCAAGAGGGTTATTATGATGTGCAGTCGGTGGCACTGCATGAGATTGGGCATCTTCTTGGCCTCGCCCACAGCAAAACCAAAGAAGCCATCATGTGGCCAGTTATACCCACCAATTTTGTCAAGGGCTTGTATTATGACGATATTGCTGGACTCAAGGCTTTATATGGATTTCCTTATTTT

Protein sequence

AEFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGVPDIFHNETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALYGFPYF

Homology

[Close]

BLAST of MS003172 vs. NCBI nr
Match: XP_022133608.1 (matrix metalloproteinase-9-like [Momordica charantia])

HSP 1 Score: 516.5 bits (1329), Expect = 1.5e-142
Identity = 256/300 (85.33%), Postives = 264/300 (88.00%), Query Frame = 0


Query: 1   AEFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIH 60
           AEFKHSLQLSLISFIL+ASIAAHVTSARD+HNHSH KSSHIIFSQNLLGTRK HNIEGIH
Sbjct: 2   AEFKHSLQLSLISFILLASIAAHVTSARDIHNHSHDKSSHIIFSQNLLGTRKSHNIEGIH 61

Query: 61  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 120
           DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNV+GILDKETLSLM QPRCGV
Sbjct: 62  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVTGILDKETLSLMSQPRCGV 121

Query: 121 PDIFHNETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYS 180
           PDIF                          ++   + I  + EGFKDPVRKALILWSAYS
Sbjct: 122 PDIF------------------------QIFSTTRRSISTW-EGFKDPVRKALILWSAYS 181

Query: 181 HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA 240
           HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA
Sbjct: 182 HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA 241

Query: 241 QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALYGFPYF 300
           QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGL+ALYGFPYF
Sbjct: 242 QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLEALYGFPYF 276

[Close]

BLAST of MS003172 vs. NCBI nr
Match: XP_022133610.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 454.1 bits (1167), Expect = 9.1e-124
Identity = 221/308 (71.75%), Postives = 246/308 (79.87%), Query Frame = 0


Query: 1   AEFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIH 60
           AEFK+SL LSLISFIL+A+IAAHVTSAR LHNH+H+KSSHIIFSQNLLG+RKGHNI+GIH
Sbjct: 2   AEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIH 61

Query: 61  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 120
            VKTYLKRYGY TH  +Q  AFDD LESALKTYQKFFNLNVSGILD ETLSLM QPRC V
Sbjct: 62  HVKTYLKRYGYFTH--TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAV 121

Query: 121 PDIFHNET---------EHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRK 180
           PDIFHNET         EHLHMGSHY+FFP   KWP+SK  LRY + HD+P  FKDPV  
Sbjct: 122 PDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNN 181

Query: 181 ALILWSAYSHFGFTEAGRDEL-DDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHL 240
           AL LWSAY  FGF E G D+   D+KISFE G HGD HPF+P +GVLAHAFAP DGRFH 
Sbjct: 182 ALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHF 241

Query: 241 NVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAG 299
           NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ D++AG
Sbjct: 242 NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAG 301

[Close]

BLAST of MS003172 vs. NCBI nr
Match: KAG6602464.1 (hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 322.4 bits (825), Expect = 4.1e-84
Identity = 165/294 (56.12%), Postives = 203/294 (69.05%), Query Frame = 0


Query: 12  ISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDVKTYLKRYGY 71
           + FIL+A+IA H   AR LH    HK S  IF Q+L+G+ KGHN+EGIH+V+ YL+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLHT---HKFSSSIFPQHLVGSGKGHNMEGIHNVRAYLQRYGY 68

Query: 72  L-THIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGVPDIFHNET-- 131
           L T++ ++  AFDD+LE ALK+YQKFFNLNVSGILD ETL LM QPRCGVPD+F NET  
Sbjct: 69  LSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFSNETGG 128

Query: 132 ------EHLHMGSHYVFF-PGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYSH 191
                 +H  +G  Y FF     KW  SKYNL+Y F+ ++PE +K  VR+A   WS+ + 
Sbjct: 129 GGDGDWDH-SIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTG 188

Query: 192 FGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQ 251
           F FTE   D   D+KISFE G HGD +PFV G+GVLAHAFAP DGR H N    W+ GA 
Sbjct: 189 FTFTEGSADN-TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADGAY 248

Query: 252 EGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
              YDV +VALHE+GH+LGL HS  + A+MWP  P NFV GL  DD+ G+ ALY
Sbjct: 249 PYKYDVGTVALHELGHVLGLGHSAFRYAVMWPYTPPNFVTGLDNDDVNGIMALY 297

[Close]

BLAST of MS003172 vs. NCBI nr
Match: XP_023544665.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.2 bits (822), Expect = 9.2e-84
Identity = 163/292 (55.82%), Postives = 204/292 (69.86%), Query Frame = 0


Query: 12  ISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDVKTYLKRYGY 71
           + F+L+A+IA H   AR LH    HK S  IF Q+L+G+ KGHNIEGIH+V+ +L+RYGY
Sbjct: 9   VMFVLVAAIAPHAIMARHLHT---HKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 72  L-THIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGVPDIFHNET-- 131
           L T++ ++  AFDD+LE ALK+YQKFFNLNVSGILD ETL LM QPRCGVPD+F NET  
Sbjct: 69  LSTNVETKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFINETSG 128

Query: 132 ----EHLHMGSHYVFF-PGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYSHFG 191
               +H  +G  Y FF     KW  SKYNL+Y F+ ++PE +K  VR+A   WS+ + F 
Sbjct: 129 EGDRDH-SIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTGFT 188

Query: 192 FTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEG 251
           FTE   D   D+KISFE G HGD +PF  G+GVLAHAFAP DGR H N  + W+ GA   
Sbjct: 189 FTEGSADN-TDVKISFEVGDHGDGYPFEDGSGVLAHAFAPEDGRLHYNAQEAWADGAYPD 248

Query: 252 YYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
            YDV +VALHE+GH+LGL HS  + A+MWP+ P NFV GL  DD+ G+ ALY
Sbjct: 249 KYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALY 295

[Close]

BLAST of MS003172 vs. NCBI nr
Match: XP_023544311.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.3 bits (817), Expect = 3.5e-83
Identity = 163/294 (55.44%), Postives = 203/294 (69.05%), Query Frame = 0


Query: 12  ISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDVKTYLKRYGY 71
           + FIL+A+IA H   AR LH    HK S  IF Q+L+G+ KGHNIEGIH+V+ +L+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLHT---HKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 72  L-THIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGVPDIFHNET-- 131
           L T++ ++  AFDD+LE ALK+YQKFFNLNVSGILD ETL LM QPRCGVPD+F NET  
Sbjct: 69  LSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETGG 128

Query: 132 ------EHLHMGSHYVFF-PGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYSH 191
                 +H  +G HY FF     KW  SKYNL+Y F+  +PE +K  V +A+I WS  S 
Sbjct: 129 DGNGDRDH-PIGLHYRFFNDSKPKWSASKYNLKYTFVRSFPEQYKSTVVQAMISWSLNSV 188

Query: 192 FGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQ 251
           F F+E   D+  D++ISFE G HGD HPF  G+GVLAHAFAP DGR H N    W+ GA 
Sbjct: 189 FAFSEGSTDD-TDVRISFEVGEHGDGHPFKKGSGVLAHAFAPEDGRLHFNEWHAWADGAY 248

Query: 252 EGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
              YDV SVALHE+GH+LGL HS  ++A+MWP +  N  K L  DD++G+ ALY
Sbjct: 249 PDKYDVGSVALHELGHILGLGHSDIRDAVMWPSMRPNTKKELSSDDVSGISALY 297

[Close]

BLAST of MS003172 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.4e-49
Identity = 107/279 (38.35%), Postives = 149/279 (53.41%), Query Frame = 0


Query: 46  NLLGTRKGHNIEGIHDVKTYLKRYGYL--THIGSQPGAFDDDLESALKTYQKFFNLNVSG 105
           N  G   G N++G++ +K Y +R+GY+  T  G+    FDD L++A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 106 ILDKETLSLMLQPRCGVPDIFHNET----------------EHLHMGSHYVFFPGNLKWP 165
            LD  T+  ++ PRCG PD+ +  +                 HLH    Y  FPG  +WP
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWP 165

Query: 166 KSKYNLRYKFIHDYP--EGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHG 225
           +++ +L Y F    P  E  K    +A   WS  +   FT +      DI I F  G HG
Sbjct: 166 RNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHG 225

Query: 226 DRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEGYY-------DVQSVALHEIGHL 285
           D  PF    G LAHAF+P  G+FHL+ D+ W V      +       D++SVA+HEIGHL
Sbjct: 226 DGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHL 285

Query: 286 LGLAHSKTKEAIMWPVIPTNFVK-GLYYDDIAGLKALYG 297
           LGL HS  +E+IM+P I T   K  L  DD+ G++ LYG
Sbjct: 286 LGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of MS003172 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.9e-48
Identity = 108/267 (40.45%), Postives = 143/267 (53.56%), Query Frame = 0


Query: 47  LLGTRKGHNIEGIHDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILD 106
           L G   G NI G+  +K Y +R+GY+T  G+    FDD L+SA+ TYQK FNL V+G LD
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLD 107

Query: 107 KETLSLMLQPRCGVPDIFHNETEH-----LHMGSHYVFFPGNLKWPKSKYNLRYKFI--H 166
             TL  +++PRCG PD+    +E      L     Y FFPG  +WPK K +L Y F   +
Sbjct: 108 SSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQN 167

Query: 167 DYPEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAH 226
           +  +  K    +A   W+  +   FT +      DI I F  G HGD  PF    G LAH
Sbjct: 168 NLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAH 227

Query: 227 AFAPTDGRFHLNVDQKWSVGAQE---------GYYDVQSVALHEIGHLLGLAHSKTKEAI 286
           A +P  G  HL+ D+ W +   E            D++SVA+HEIGHLLGL HS  ++AI
Sbjct: 228 ASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAI 287

Query: 287 MWPVIPTNFVK-GLYYDDIAGLKALYG 297
           M+P I     K  L  DDI G++ LYG
Sbjct: 288 MFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of MS003172 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 2.9e-48
Identity = 106/255 (41.57%), Postives = 136/255 (53.33%), Query Frame = 0


Query: 53  GHNIEGIHDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSL 112
           G N +G+ +VK Y    GY+ +       FDD L SA+KTYQK +NLNV+G  D  TL  
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQ 107

Query: 113 MLQPRCGVPDIF--HNETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHD--YPEGFKDP 172
           ++ PRCGVPDI    N+T    M S Y FF    +W      L Y F  +    + FK  
Sbjct: 108 IMTPRCGVPDIIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSA 167

Query: 173 VRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRF 232
           + +A   W+   +  F E    E  +IKI F    HGD +PF    G+L HAFAPTDGR 
Sbjct: 168 IARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRC 227

Query: 233 HLNVDQKWSVGAQ------EGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVK- 292
           H + D+ W              +D++SVA+HEIGHLLGL HS    AIM+P IP    K 
Sbjct: 228 HFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKV 287

Query: 293 GLYYDDIAGLKALYG 297
            L  DDI G++ LYG
Sbjct: 288 NLAQDDIDGIRKLYG 302

[Close]

BLAST of MS003172 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.7e-46
Identity = 103/282 (36.52%), Postives = 145/282 (51.42%), Query Frame = 0


Query: 46  NLLGTRKGHNIEGIHDVKTYLKRYGYLTHI---GSQPGAFDDDLESALKTYQKFFNLNVS 105
           N  G   G   +G++ +K Y + +GY+T     G+    FDD L++A++ YQ+ F LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 106 GILDKETLSLMLQPRCGVPDI-----------------FHNETEHLHMGSHYVFFPGNLK 165
           G+LD+ TL  ++ PRCG PD+                 F    +  H   HY FFPG  +
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 166 WPKSKYNLRYKF--IHDYPEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGA 225
           WP+++ +L Y F   +   E  K    +A   W   +   FT   R    DI I F  G 
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 226 HGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEG--------YYDVQSVALHEI 285
           HGD  PF      LAHAF+P  G FHL+ ++ W V  + G          D++SVA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 286 GHLLGLAHSKTKEAIMWPVIPTNFVK-GLYYDDIAGLKALYG 297
           GHLLGL HS  + +IM+P I T   K  L  DD+ G++ LYG
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of MS003172 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.4e-44
Identity = 104/263 (39.54%), Postives = 139/263 (52.85%), Query Frame = 0


Query: 47  LLGTRKGHNIEGIHDVKTYLKRYGYLTHIGSQ--PGAFDDDLESALKTYQKFFNLNVSGI 106
           L+  + G ++ G+ ++K YL R+GY+   GS+     FD  LESA+  YQ+   L ++G 
Sbjct: 58  LVDVQIGSHVSGVSELKRYLHRFGYVND-GSEIFSDVFDGPLESAISLYQENLGLPITGR 117

Query: 107 LDKETLSLMLQPRCGVPDIFHN-ETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIH--DY 166
           LD  T++LM  PRCGV D       + LH  +HY +F G  KW +          H  DY
Sbjct: 118 LDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKWNRDTLTYAISKTHKLDY 177

Query: 167 --PEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAH 226
              E  K   R+A   WS+     F E       D+KI F  G HGD  PF    G LAH
Sbjct: 178 LTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAH 237

Query: 227 AFAPTDGRFHLNVDQKWSV-----GAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPV 286
           AFAP +GR HL+  + W V     G+ E   D++SVA HEIGHLLGL HS  + A+M+P 
Sbjct: 238 AFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPS 297

Query: 287 I-PTNFVKGLYYDDIAGLKALYG 297
           + P      L  DD+AG+  LYG
Sbjct: 298 LRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of MS003172 vs. ExPASy TrEMBL
Match: A0A6J1BZL4 (matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.2e-143
Identity = 256/300 (85.33%), Postives = 264/300 (88.00%), Query Frame = 0


Query: 1   AEFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIH 60
           AEFKHSLQLSLISFIL+ASIAAHVTSARD+HNHSH KSSHIIFSQNLLGTRK HNIEGIH
Sbjct: 2   AEFKHSLQLSLISFILLASIAAHVTSARDIHNHSHDKSSHIIFSQNLLGTRKSHNIEGIH 61

Query: 61  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 120
           DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNV+GILDKETLSLM QPRCGV
Sbjct: 62  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVTGILDKETLSLMSQPRCGV 121

Query: 121 PDIFHNETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYS 180
           PDIF                          ++   + I  + EGFKDPVRKALILWSAYS
Sbjct: 122 PDIF------------------------QIFSTTRRSISTW-EGFKDPVRKALILWSAYS 181

Query: 181 HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA 240
           HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA
Sbjct: 182 HFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGA 241

Query: 241 QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALYGFPYF 300
           QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGL+ALYGFPYF
Sbjct: 242 QEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLEALYGFPYF 276

[Close]

BLAST of MS003172 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.4e-124
Identity = 221/308 (71.75%), Postives = 246/308 (79.87%), Query Frame = 0


Query: 1   AEFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIH 60
           AEFK+SL LSLISFIL+A+IAAHVTSAR LHNH+H+KSSHIIFSQNLLG+RKGHNI+GIH
Sbjct: 2   AEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIH 61

Query: 61  DVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 120
            VKTYLKRYGY TH  +Q  AFDD LESALKTYQKFFNLNVSGILD ETLSLM QPRC V
Sbjct: 62  HVKTYLKRYGYFTH--TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAV 121

Query: 121 PDIFHNET---------EHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRK 180
           PDIFHNET         EHLHMGSHY+FFP   KWP+SK  LRY + HD+P  FKDPV  
Sbjct: 122 PDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNN 181

Query: 181 ALILWSAYSHFGFTEAGRDEL-DDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHL 240
           AL LWSAY  FGF E G D+   D+KISFE G HGD HPF+P +GVLAHAFAP DGRFH 
Sbjct: 182 ALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHF 241

Query: 241 NVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAG 299
           NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ D++AG
Sbjct: 242 NVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAG 301

[Close]

BLAST of MS003172 vs. ExPASy TrEMBL
Match: A0A6J1BX62 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006146 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 4.2e-82
Identity = 162/298 (54.36%), Postives = 199/298 (66.78%), Query Frame = 0


Query: 3   FKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDV 62
           FK SLQL ++ F+L+A IA HV  ARDL     HKS   +F Q L G+RKGHNI+GIHDV
Sbjct: 3   FKFSLQLQVL-FLLLACIAPHVILARDL---GTHKSYRRLFPQYLEGSRKGHNIQGIHDV 62

Query: 63  KTYLKRYGYLTHIGSQP--GAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 122
           K YL+RYGY++    QP   AFDD LES LK YQ+FF LNVSGIL++ETL LM QPRCG 
Sbjct: 63  KLYLQRYGYMSANSVQPNDNAFDDALESGLKKYQQFFKLNVSGILERETLVLMSQPRCGC 122

Query: 123 PDIF---HNETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWS 182
            D+F   ++E    +  SHY FFP N KWP +++ L Y FIH++P  F  PV +A   W 
Sbjct: 123 SDMFDAENHEVNKANTSSHYTFFPRNRKWPANRHYLTYGFIHNFPTNFMTPVVRAFEDWD 182

Query: 183 AYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWS 242
             + F FT+A   +  +I +SFERG HGDR PF    GVLAHAF P DGR H + D+ W 
Sbjct: 183 FNTLFSFTQAPHVQAANIVLSFERGYHGDRFPFDGRWGVLAHAFGPNDGRVHFDADENWV 242

Query: 243 VGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
           VG     +DVQ VALHE+GH+LGL HS   +AIMWP +  N VKGL  DDI G++ALY
Sbjct: 243 VGMVADGFDVQMVALHELGHVLGLKHSADSQAIMWPYMEPNTVKGLSGDDIEGIRALY 296

[Close]

BLAST of MS003172 vs. ExPASy TrEMBL
Match: A0A6J1H7Q4 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.6e-81
Identity = 163/296 (55.07%), Postives = 201/296 (67.91%), Query Frame = 0


Query: 2   EFKHSLQLSLISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHD 61
           + K  LQL    FIL+A+IA H   AR LH    HK S  IF Q+L+G+ KGHN+EGIH+
Sbjct: 2   DLKSCLQL---MFILVAAIAPHAIMARHLHT---HKFSSSIFPQHLVGSGKGHNMEGIHN 61

Query: 62  VKTYLKRYGYL-THIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGV 121
           V+ +L+RYGYL T++ ++  AFDD+LE ALK+YQKFFNLNVSGILD ETL LM QPRCGV
Sbjct: 62  VRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGV 121

Query: 122 PDIFHNETEHLHMGSHYVFF-PGNLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAY 181
           PD+F  +         Y FF     KW  SKYNL+Y F+ ++PE +K  VR+A   WS+ 
Sbjct: 122 PDVFRID---------YTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSV 181

Query: 182 SHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVG 241
           + F FTE   D   D+KISFE G HGD +PFV G+GVLAHAFAP DGR H N    W+ G
Sbjct: 182 TGFTFTEGSVDN-TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADG 241

Query: 242 AQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
           A    YDV +VALHE+GH+LGL HS  + A+MWP  P NFV GL  DD+ G+ ALY
Sbjct: 242 AYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVNGIMALY 281

[Close]

BLAST of MS003172 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 2.3e-80
Identity = 158/294 (53.74%), Postives = 199/294 (67.69%), Query Frame = 0


Query: 12  ISFILMASIAAHVTSARDLHNHSHHKSSHIIFSQNLLGTRKGHNIEGIHDVKTYLKRYGY 71
           + FIL+A+IA H   AR LH    HK S  IF Q+L+G+ KGHNIEGIH+V+ +L+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLH---IHKLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 72  L-THIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRCGVPDIFHNET-- 131
           L T++ ++   FDD+LE A+K+YQKFFNLNVSGILD ETL LM QPRCGVPD+F NET  
Sbjct: 69  LSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETGG 128

Query: 132 ------EHLHMGSHYVFFPG-NLKWPKSKYNLRYKFIHDYPEGFKDPVRKALILWSAYSH 191
                 +H  +G HY FF     KW  SKYNL+Y F+  +PE +K+ V +A+I WS  S 
Sbjct: 129 DGNGDRDH-PIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMISWSFNSV 188

Query: 192 FGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQ 251
           F F+E   D   D++ISFE G HGD  PF    G LAHAFAP DGR H N    W+ GA 
Sbjct: 189 FAFSEGSTDN-TDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAWADGAY 248

Query: 252 EGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIAGLKALY 296
              +DV SVALHE+GH+LGL HS  ++A+MWP +P N  K L  DD++G+ ALY
Sbjct: 249 PDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALY 297

[Close]

BLAST of MS003172 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 196.8 bits (499), Expect = 2.4e-50
Identity = 107/279 (38.35%), Postives = 149/279 (53.41%), Query Frame = 0


Query: 46  NLLGTRKGHNIEGIHDVKTYLKRYGYL--THIGSQPGAFDDDLESALKTYQKFFNLNVSG 105
           N  G   G N++G++ +K Y +R+GY+  T  G+    FDD L++A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 106 ILDKETLSLMLQPRCGVPDIFHNET----------------EHLHMGSHYVFFPGNLKWP 165
            LD  T+  ++ PRCG PD+ +  +                 HLH    Y  FPG  +WP
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWP 165

Query: 166 KSKYNLRYKFIHDYP--EGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHG 225
           +++ +L Y F    P  E  K    +A   WS  +   FT +      DI I F  G HG
Sbjct: 166 RNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHG 225

Query: 226 DRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEGYY-------DVQSVALHEIGHL 285
           D  PF    G LAHAF+P  G+FHL+ D+ W V      +       D++SVA+HEIGHL
Sbjct: 226 DGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHL 285

Query: 286 LGLAHSKTKEAIMWPVIPTNFVK-GLYYDDIAGLKALYG 297
           LGL HS  +E+IM+P I T   K  L  DD+ G++ LYG
Sbjct: 286 LGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of MS003172 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 193.7 bits (491), Expect = 2.1e-49
Identity = 108/267 (40.45%), Postives = 143/267 (53.56%), Query Frame = 0


Query: 47  LLGTRKGHNIEGIHDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILD 106
           L G   G NI G+  +K Y +R+GY+T  G+    FDD L+SA+ TYQK FNL V+G LD
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLD 107

Query: 107 KETLSLMLQPRCGVPDIFHNETEH-----LHMGSHYVFFPGNLKWPKSKYNLRYKFI--H 166
             TL  +++PRCG PD+    +E      L     Y FFPG  +WPK K +L Y F   +
Sbjct: 108 SSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQN 167

Query: 167 DYPEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAH 226
           +  +  K    +A   W+  +   FT +      DI I F  G HGD  PF    G LAH
Sbjct: 168 NLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAH 227

Query: 227 AFAPTDGRFHLNVDQKWSVGAQE---------GYYDVQSVALHEIGHLLGLAHSKTKEAI 286
           A +P  G  HL+ D+ W +   E            D++SVA+HEIGHLLGL HS  ++AI
Sbjct: 228 ASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAI 287

Query: 287 MWPVIPTNFVK-GLYYDDIAGLKALYG 297
           M+P I     K  L  DDI G++ LYG
Sbjct: 288 MFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of MS003172 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-47
Identity = 103/282 (36.52%), Postives = 145/282 (51.42%), Query Frame = 0


Query: 46  NLLGTRKGHNIEGIHDVKTYLKRYGYLTHI---GSQPGAFDDDLESALKTYQKFFNLNVS 105
           N  G   G   +G++ +K Y + +GY+T     G+    FDD L++A++ YQ+ F LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 106 GILDKETLSLMLQPRCGVPDI-----------------FHNETEHLHMGSHYVFFPGNLK 165
           G+LD+ TL  ++ PRCG PD+                 F    +  H   HY FFPG  +
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 166 WPKSKYNLRYKF--IHDYPEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGA 225
           WP+++ +L Y F   +   E  K    +A   W   +   FT   R    DI I F  G 
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 226 HGDRHPFVPGTGVLAHAFAPTDGRFHLNVDQKWSVGAQEG--------YYDVQSVALHEI 285
           HGD  PF      LAHAF+P  G FHL+ ++ W V  + G          D++SVA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 286 GHLLGLAHSKTKEAIMWPVIPTNFVK-GLYYDDIAGLKALYG 297
           GHLLGL HS  + +IM+P I T   K  L  DD+ G++ LYG
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of MS003172 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.1e-45
Identity = 104/263 (39.54%), Postives = 139/263 (52.85%), Query Frame = 0


Query: 47  LLGTRKGHNIEGIHDVKTYLKRYGYLTHIGSQ--PGAFDDDLESALKTYQKFFNLNVSGI 106
           L+  + G ++ G+ ++K YL R+GY+   GS+     FD  LESA+  YQ+   L ++G 
Sbjct: 58  LVDVQIGSHVSGVSELKRYLHRFGYVND-GSEIFSDVFDGPLESAISLYQENLGLPITGR 117

Query: 107 LDKETLSLMLQPRCGVPDIFHN-ETEHLHMGSHYVFFPGNLKWPKSKYNLRYKFIH--DY 166
           LD  T++LM  PRCGV D       + LH  +HY +F G  KW +          H  DY
Sbjct: 118 LDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKWNRDTLTYAISKTHKLDY 177

Query: 167 --PEGFKDPVRKALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAH 226
              E  K   R+A   WS+     F E       D+KI F  G HGD  PF    G LAH
Sbjct: 178 LTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAH 237

Query: 227 AFAPTDGRFHLNVDQKWSV-----GAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPV 286
           AFAP +GR HL+  + W V     G+ E   D++SVA HEIGHLLGL HS  + A+M+P 
Sbjct: 238 AFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPS 297

Query: 287 I-PTNFVKGLYYDDIAGLKALYG 297
           + P      L  DD+AG+  LYG
Sbjct: 298 LRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of MS003172 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 172.6 bits (436), Expect = 4.9e-43
Identity = 96/250 (38.40%), Postives = 133/250 (53.20%), Query Frame = 0


Query: 59  IHDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVSGILDKETLSLMLQPRC 118
           I ++K +L++YGYL        + D   E AL  YQK   L ++G  D +TLS +L PRC
Sbjct: 50  IPEIKRHLQQYGYLP---QNKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRC 109

Query: 119 GVPDIFHNETEHLHMGSHYVFFPGNLKWPKS-KYNLRYKFIHD------YPEGFKDPVRK 178
           G PD    +T   H G  YV+FPG  +W +     L Y F  +       P   +   R+
Sbjct: 110 GFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRR 169

Query: 179 ALILWSAYSHFGFTEAGRDELDDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFHLN 238
           A   W++     F E     + DIKI F  G HGD  PF    GVLAH F+P +GR HL+
Sbjct: 170 AFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLD 229

Query: 239 VDQKWSVGAQEG----YYDVQSVALHEIGHLLGLAHSKTKEAIMWPVI-PTNFVKGLYYD 297
             + W+V   E       D++SVA+HEIGH+LGL HS  K+A M+P + P +    L  D
Sbjct: 230 KAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMD 289

The following BLAST results are available for this feature:

BLAST of MS003172 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133608.1	1.5e-142	85.33	matrix metalloproteinase-9-like [Momordica charantia]	[more]
XP_022133610.1	9.1e-124	71.75	metalloendoproteinase 1-like [Momordica charantia]	[more]
KAG6602464.1	4.1e-84	56.12	hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023544665.1	9.2e-84	55.82	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023544311.1	3.5e-83	55.44	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS003172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04529	3.4e-49	38.35	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	2.9e-48	40.45	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
P29136	2.9e-48	41.57	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q5XF51	2.7e-46	36.52	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O23507	4.4e-44	39.54	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of MS003172 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZL4	7.2e-143	85.33	matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 P...	[more]
A0A6J1BVR3	4.4e-124	71.75	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]
A0A6J1BX62	4.2e-82	54.36	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006146 PE=3...	[more]
A0A6J1H7Q4	1.6e-81	55.07	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 ...	[more]
A0A6J1H7I1	2.3e-80	53.74	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ...	[more]

BLAST of MS003172 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70170.1	2.4e-50	38.35	matrix metalloproteinase	[more]
AT1G59970.1	2.1e-49	40.45	Matrixin family protein	[more]
AT1G24140.1	1.9e-47	36.52	Matrixin family protein	[more]
AT4G16640.1	3.1e-45	39.54	Matrixin family protein	[more]
AT2G45040.1	4.9e-43	38.40	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 113..126 score: 55.75 coord: 250..275 score: 55.63 coord: 169..184 score: 31.78 coord: 193..221 score: 51.81 coord: 283..296 score: 52.53
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 142..297 e-value: 8.8E-22 score: 88.4
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 50..298 e-value: 6.3E-68 score: 230.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 57..113 e-value: 8.3E-15 score: 54.7
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 150..296 e-value: 4.7E-35 score: 120.8
None	No IPR available	PIRSR	PIRSR001191-1	PIRSR001191-1	coord: 59..296 e-value: 7.4E-55 score: 184.3
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 22..296
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 22..296
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 139..298
IPR021158	Peptidase M10A, cysteine switch, zinc binding site	PROSITE	PS00546	CYSTEINE_SWITCH	coord: 116..123
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 52..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003172.1	MS003172.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0008270	zinc ion binding