MS003153 (gene) Bitter gourd (TR) v1

Overview


Name	MS003153
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Folylpolyglutamate synthase
Location	scaffold234: 764875 .. 771087 (+)
RNA-Seq Expression	MS003153
Synteny	MS003153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTTTGAAGGTAATGGCACAATGAAATACTCGCAGACCGCCTATGAAGAGGCATTGGATGCACTATCCTCTTTGATTGTCAACCGTAGACGATGTGATAAATCTGGAGATCGATTTGAGTTGATGTTCGAGTATGTAAAGGTATTACTGAAACTTAACACTTCATTATTGCTGAGTGTACTTGGGCTGATGATTATTGAGTTCACTTAATATAATAGATGCTGGATTTGGAGGAGCCCATGTTGCAGATGAAGGTCATTCATGTCGCTGGCACAAAAGGCAAGGTACCAATTTTCTTTTTCGAAAGAAATATTTTCAGAGCACAAAGGGATTCTAGTCATGTATAGACGTTTATAGTTTCTTCATCTGTTTTATTAGAACATTCATTTGTGCTATATTTTATGTATGTAAACACTTCGACGCCATTTTATTCTGTTATCATAAATTTTAATGAAATAACCAGATTTCTTAGTTATTTACTTTCAAGTATATTCCATATTTATGAAAATAAAAGGGATATTAGATGTATGTTACAAACTTCTTTTAGAAAATTAATTTAGTTTAAAAGTCTTGTTTCTTGTAAAAATAAGGGTTATGGTTTTATACTTTTACGTCTCATTATATAGCTTACACCTTACACTTTGATTTTATTTGATAAATAGAGGTACATAATGGTTCTGGTTGAGAATGCAATCAATATATAAATTTATCTCAATCTAAAGTGTTTTAGGTCTAATAATAATTTAACAAGATATCAAAGCAAGAAGTATGAAAATCAAACTCCTGTGCATATTATTTCCTTCCCATTTCATATTAAGAATTCATGTGTAGTGCCTTGTGTTGATATCTAAGCTGACATGTGGGATGTAATGTTGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTGTTTTGTGACTTTCAAATTTCTTGCACACTGCTAATTGTCAATTGAAGAAGATTTTTACTTATTTGCTTAGAAATCTATCATTGAGATTGCTTGAAAATCATATTGTGTTAACTGAACTTTTTTCTTTTTGTTGGGACTTGCATAGGGATCCACGTGCACCTTTACTGAATCCATATTGCGTAATTGTGGCTTCCACACTGGCCTTTTCACTTCGCCTCATCTTATTGATATCCGAGAAAGATTTCGCCTGGATGGGTAACTATTTTACATATTACATGGTTTGGTCGTTCTTTTTTTTTTAGATGAGGCAGATGGTTGTTCTCATTGTAAAGGCAATGATTTTCAACTGCTTGAACAACTTGCATCACATTATCTAATTTTATTTCTGGACCAAGTGCTTTTGTTCGAAAAGTTTAAGCTCTTTTTCAATTTTGTAGTTGCAGACTCTTGAGTGCCCTTCTGATAATTGGTTATAAATGTTGTTTTAATGATTATTTTTTGCTTCAATTGGTTGTTGCAGTATCGACATTAGTGAAGAGAAATTTTTAACCTATTTCTGGTGGTGCTATAATAGGTTAAAGGTATTTTCCTTGTCATTTTGATTTACTATTATTTTTCTAAGGCATAAGTTCATCTAATGGATAACATTTATGCTGCTTTGAATTTAATTGCACATGCAAAAGTTGTATTTAGGTTCCGAGTTGCTTATTCTTATAGCAAAGAGAATTTTGAACACTATAAAACTAAATTCTGAACTTAACTAAACTAATTCTATATGGCTATTTCGTAATTTATTTCCGCTTAATCAAAGCAACTCTTATAGGGGGAAAAAATCTGCTATGTAGAACTGACGGATGTTGTGTCATTTTGTTGCATGCCAGAATATATTGGAATATTGATTCTTATGTTATATTTATTATGAGTGTGTAACACAAGGTTAGATAGTGGAAGGGAGATTGAAACTTTGTAGCTAGAATCATGTATTTGGAGTTTTAGCTTCAAGTAGGGTAAGATCTATAACCAACCTATTAAAGAATTATATTGGCTTTTTGTATCTTTCACTAGAAACAGAACGTTTCACTGATATTAAAAAAAAGTCACGAGAAGAATAACAGGCTTGTTTATGTTATCAAGGTTATGAGACAGGGTAACAAATACAATCTTAGAATAAATCCGGGTTCATTTCAAAAGGCCCCTCAACAAGAATTAATTTCACTATAAGAATAAATACAAAAAGGGCTTGATGTGGAATACCCTAAAGAAGTATGACTTTTGGTCCAATCCGTCCTTTCACTCCAACATAATGAATTATGTTGGATTTGATTGAGCATCTGCCACATATCTTATATTTAATCTAATAAGTTTTGTGCATTACATTGTCTTTAAGCTTTATTTCTAACTCTTTCGAGCTCAAATCTAGAGGAAATTGGTCATGCTGTTATTATTTTTGCATCCAGATTAATTATGGACAGTCATCCAACTGTATTGTATCTCTCAGAGTACTAAAAACTTACATGCTGGCAATCCTGTATTGATATGATAACTAAAGTGGTTCCATTTAGGATTTGTTGGACTATCAATTTTCCTAGCTGTTCACTATGATTCTATATGATTTTTATTTTCTGCAGGAAAAGACTAGTAAGGATATACCAATGCCTCAATTGTTTCGCTTCCTTGCATTACTTGCGTTCAAAATATTTTCAGATGAGCAGGTTTGATGTGTACTTTTTATTTTAATCTTTTTTTGGACAGGCTGTCTTTGCTCGATTTATTAGGTGGATTAGAAAGATAATATTTTATTGCCATCAATGAAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTCGATGCTACAAATGTGGTATGCTCATTCTCTTTTGGTTTGAATAAATAAGCTATTACGTGGCATTTCAGAGAATTTGTTCTTCATCTATTATTTAAAGTAGAAACCTTACATCAAAGTATGGCCATTATAAGATCCTCTAATGTCTCAGGTCCGGGCACCCATTGTGTGTGGTATAGCTTCTCTTGGTTACGACCACATGGAACTTCTTGGTTAGCCTCCTCTTAACCTTGATGGTTGTTTTCCTGGAAAGTAAATGATTTTAACTGGTTGCTTTAGTTTGGCTTATGCATGAGCATAGACGTTTTGTCTCCTGTTTGATAAGTGCTCCAATTCACATTTCATATGTTTTTTAAATTGTTGCTTTCTTTCTGTTTTGCATTTGTCGAAATAGGAAATACTCTTGGTGAGATAGCTTCGGAGAAAGCTGGTATCTTCAAGGTTTGCTTCAAAAGACTTTCCTCAAAGCACAAACATCTTGGAATAATCAATTAATGTTTGTTGTCTTCCTTTTCCTTTGGTGCAGCGTGGTGTTCCAGCTTTTACCGTAGTTCAACCTGATGAAGCCATGAATGTGCTTAGAGATAAGGCTTCACAGTTGGATGTATGTGGATGGTTCTTCCATTAACTAGTCCAGTTCATTCACAAAAACACATGCATGACTTCCTTTGTACCATTACTAGAAGAGCTGACACATAAAACCTAAATTTAATTTTAGTACACCCTTGTTAGTAGCCATTGTTCATAGCACCTTATATTTGAAACAGTGTCATGTTAACTTTGGATGCTCTGAATTTTCTTTCTTTGCTAGGCCATATGGGAGCTGAAATTATTTCTTGTAGTAATCTATGCTTCTGTATGTCTCACATGGCAATGTAACTACTAAATTACATCAATTAGTTAGCCGTTTTGTTTTTCTTTTCTTTTGGTTTCTGTTTTTTTTTTTAGGATTCCTGATAAGGTAACAGTATTTTTGATTGCGCAAGGCTGATTGTTACCCTACCAGCTAGGAGGTTTACGGTACAACCTTTCCTCTAAATGAACTGCAACAATTTTGCACATCATAGGTACAACTAACAGTAGTGCCGTCGTTGGATCCTGAGCTACTACATGGTCATAAACTTGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTGGTCTTGCTATTGCCCTATCCTCCGCTTGGCTCCAGAGGACTGGCCATTCCAAGCTGTCATACATGGAACAGAGTGTAAGTTCTACACATGAGATTTTTCTTCTATAACATTATCTCGTGTTTTATGATTCTTTTTCCTTTTAATAATATGTTATCAGAGGTCTTTGCCCGAGCAGTTCATAGAAGGGCTTACAACTGCCAGCTTTGAAGGACGAGCACAAATTGTTCCTGATAGATATATTGATATTGAATCCCCGGGAGATCTTGTCTTTTATTTGGATGGAGCCCATAGTCCAGAGAGTATGGAGGCTTGCGGTAGATGGTTTTCTGCTGTGATGAAAGATAACCAGCGAGGCACCTTAAATTATCAGTTGGAGGATAATGGTATATCTTCAAATAAACTTCTTCAAGAAAATCAGAATGATGAAGCAAGGAAGACATCTGTGCCGGTAAATTTCAACTTTAGTTCCACTCTTCCAAGTTCATTTTATTGTTTGTCAAGAAAAAGAGGATTATAAATATTTAAATTAGATAGTTTCAGGTTGAGTACCTATTGAGAAGGCATTGTTGGCCTGCAATCAATGTATCAAAAAGTGGGAGATCAGGGAGCAATGAAAATTTTCTTTGCTAGTATTAACAAAATATAACTCCCCTAGCATTTCTCCCCTTTCCTCTTCTTGTTTCACCCTATAGCCTCTTCTAGCAGCCTCTACTGTTGCTCTCTTCACTGTATTCTTAGTGTTACAGCCGTCTATTACTCATAGCATAAGGGATTCCAGGATTATGATTGAGAATTAAGATTCAGATACCTACTGTTAGGAGTAGGACCCCTTGTTGTCTTCCTTTGGTGCAGCCTGGGGTTCCAGGACTGCAACCTTTCTTAGAGAAGAAGAAGCTCACAAGCTGATCGGTTCCCTCTCTCATCACTATTCCTTTGAGTAATAGGCTAATAGCACAAGGGAAAAATAGTTCGATCTCTTTGACACATCTTACTTCCTTACTAGATTTTATTCATGTACAATTATTTGACTCTAAGTTCATCAGTGTAATGTTTTGACGCTCCTTTTTTAGTTCTACCTTTCATAAAGAGAAGGTATCTGACTCTTTACTCATTTATGCGGTGCTTCAACTTCTTTAGATGCTGACATGCTGTTTAACCTAGTATTGTTGGACATTGTAGGTGCTACTGTTCAATTGTATGTCAGTGCGAGATCCGCAGCTGCTTCTTCCACCTCTGATCAAAACATGTGCGAATAACGGCAAGTATTTCTCAAACTGTTAGAACTAAAAGATCAGGTGATAGATTTTTCGCAGAAGAAGATGTGCCCTAGATCAATAAAATTCCGTAAAAGTTTGCTCATATAATCCAGGTGTCCACTTCAAGAAAGCACTCTTCGTTCCAAATACATCAGTCTTCGACAAGGTTGGAACCCATGCTCTAACAGAAGCAAATGGACAAATTGACCTTTCTTGGCAGTTTAATATTCGAAGAGTATGGGAAAACCTCATCCAATGTGACAAAGGTATTATTGGCTAACGCTAGTGGTTTATGATGTAACCAAACTTGTTTCTTAAAAGTGCTGGAACCAATCTTTATCCTTCCCCAGATAGATTTTTAGTTAGATACACCATTTTAGCAAGTTTAATTCTTATACCGTGATATTTTGACACAGTAGGCACTGATTCTAAAGGGACCGGTGCTGTACTTGAAGAAGAAACCGATAAACCAGAAGTGGGTGGTAAAAGTTGTGAGAATAGCACAGTATTTCCTTCTCTCCCTGTGGCTATTAGATGGCTCCGAGATAGCATCCTGCAGCAAAACCGGTCAATTCGACTTCAGGTAATTTTGAAGAAAATGAGAATATTTTAGGTTGAGTAATTTAGGAAAATGATTATATCGTATTTGGGAGGCTATGCAACTTATTGGGGAACAGTAAACCTAAGTCAAAGAACTGAAGTTGGCTTTTGTGTTACATGTGTTCCTAAAGTATAAATCATTATCCCCAATATGAGATTTTTAGGCCAAAGGAATTATGAAGCTTGCTGGGAAAAAGAGAGAAAAACAAACTTCCGTTTCTCATTCTATGTCAATTTTTGAGGTTGACGTATCATAACATTGTGAACAAGCATTAATATTTAAATTGGTTGTTTTTCTTCCGCGGTTGTAATTTAAATTGAACTGCTTTTCATTCTTCAGGTTCTTGTAACTGGTTCCTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAG

mRNA sequence

GCTTTTGAAGGTAATGGCACAATGAAATACTCGCAGACCGCCTATGAAGAGGCATTGGATGCACTATCCTCTTTGATTGTCAACCGTAGACGATGTGATAAATCTGGAGATCGATTTGAGTTGATGTTCGAGTATGTAAAGATGCTGGATTTGGAGGAGCCCATGTTGCAGATGAAGGTCATTCATGTCGCTGGCACAAAAGGCAAGGGATCCACGTGCACCTTTACTGAATCCATATTGCGTAATTGTGGCTTCCACACTGGCCTTTTCACTTCGCCTCATCTTATTGATATCCGAGAAAGATTTCGCCTGGATGGTATCGACATTAGTGAAGAGAAATTTTTAACCTATTTCTGGTGGTGCTATAATAGGTTAAAGGAAAAGACTAGTAAGGATATACCAATGCCTCAATTGTTTCGCTTCCTTGCATTACTTGCGTTCAAAATATTTTCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTCGATGCTACAAATGTGGTCCGGGCACCCATTGTGTGTGGTATAGCTTCTCTTGGTTACGACCACATGGAACTTCTTGGAAATACTCTTGGTGAGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGTGTTCCAGCTTTTACCGTAGTTCAACCTGATGAAGCCATGAATGTGCTTAGAGATAAGGCTTCACAGTTGGATGTACAACTAACAGTAGTGCCGTCGTTGGATCCTGAGCTACTACATGGTCATAAACTTGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTGGTCTTGCTATTGCCCTATCCTCCGCTTGGCTCCAGAGGACTGGCCATTCCAAGCTGTCATACATGGAACAGAGTAGGTCTTTGCCCGAGCAGTTCATAGAAGGGCTTACAACTGCCAGCTTTGAAGGACGAGCACAAATTGTTCCTGATAGATATATTGATATTGAATCCCCGGGAGATCTTGTCTTTTATTTGGATGGAGCCCATAGTCCAGAGAGTATGGAGGCTTGCGGTAGATGGTTTTCTGCTGTGATGAAAGATAACCAGCGAGGCACCTTAAATTATCAGTTGGAGGATAATGGTATATCTTCAAATAAACTTCTTCAAGAAAATCAGAATGATGAAGCAAGGAAGACATCTGTGCCGGTGCTACTGTTCAATTGTATGTCAGTGCGAGATCCGCAGCTGCTTCTTCCACCTCTGATCAAAACATGTGCGAATAACGGCAAGTATTTCTCAAACTGTGTCCACTTCAAGAAAGCACTCTTCGTTCCAAATACATCAGTCTTCGACAAGGTTGGAACCCATGCTCTAACAGAAGCAAATGGACAAATTGACCTTTCTTGGCAGTTTAATATTCGAAGAGTATGGGAAAACCTCATCCAATGTGACAAAGTAGGCACTGATTCTAAAGGGACCGGTGCTGTACTTGAAGAAGAAACCGATAAACCAGAAGTGGGTGGTAAAAGTTGTGAGAATAGCACAGTATTTCCTTCTCTCCCTGTGGCTATTAGATGGCTCCGAGATAGCATCCTGCAGCAAAACCGGTCAATTCGACTTCAGGTTCTTGTAACTGGTTCCTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAG

Coding sequence (CDS)

GCTTTTGAAGGTAATGGCACAATGAAATACTCGCAGACCGCCTATGAAGAGGCATTGGATGCACTATCCTCTTTGATTGTCAACCGTAGACGATGTGATAAATCTGGAGATCGATTTGAGTTGATGTTCGAGTATGTAAAGATGCTGGATTTGGAGGAGCCCATGTTGCAGATGAAGGTCATTCATGTCGCTGGCACAAAAGGCAAGGGATCCACGTGCACCTTTACTGAATCCATATTGCGTAATTGTGGCTTCCACACTGGCCTTTTCACTTCGCCTCATCTTATTGATATCCGAGAAAGATTTCGCCTGGATGGTATCGACATTAGTGAAGAGAAATTTTTAACCTATTTCTGGTGGTGCTATAATAGGTTAAAGGAAAAGACTAGTAAGGATATACCAATGCCTCAATTGTTTCGCTTCCTTGCATTACTTGCGTTCAAAATATTTTCAGATGAGCAGGTGGATGTAGCTATCTTGGAGGTTGGTTTAGGTGGAACTTTCGATGCTACAAATGTGGTCCGGGCACCCATTGTGTGTGGTATAGCTTCTCTTGGTTACGACCACATGGAACTTCTTGGAAATACTCTTGGTGAGATAGCTTCGGAGAAAGCTGGTATCTTCAAGCGTGGTGTTCCAGCTTTTACCGTAGTTCAACCTGATGAAGCCATGAATGTGCTTAGAGATAAGGCTTCACAGTTGGATGTACAACTAACAGTAGTGCCGTCGTTGGATCCTGAGCTACTACATGGTCATAAACTTGGGCTTGAAGGCGAGCACCAGTATCTAAATGCTGGTCTTGCTATTGCCCTATCCTCCGCTTGGCTCCAGAGGACTGGCCATTCCAAGCTGTCATACATGGAACAGAGTAGGTCTTTGCCCGAGCAGTTCATAGAAGGGCTTACAACTGCCAGCTTTGAAGGACGAGCACAAATTGTTCCTGATAGATATATTGATATTGAATCCCCGGGAGATCTTGTCTTTTATTTGGATGGAGCCCATAGTCCAGAGAGTATGGAGGCTTGCGGTAGATGGTTTTCTGCTGTGATGAAAGATAACCAGCGAGGCACCTTAAATTATCAGTTGGAGGATAATGGTATATCTTCAAATAAACTTCTTCAAGAAAATCAGAATGATGAAGCAAGGAAGACATCTGTGCCGGTGCTACTGTTCAATTGTATGTCAGTGCGAGATCCGCAGCTGCTTCTTCCACCTCTGATCAAAACATGTGCGAATAACGGCAAGTATTTCTCAAACTGTGTCCACTTCAAGAAAGCACTCTTCGTTCCAAATACATCAGTCTTCGACAAGGTTGGAACCCATGCTCTAACAGAAGCAAATGGACAAATTGACCTTTCTTGGCAGTTTAATATTCGAAGAGTATGGGAAAACCTCATCCAATGTGACAAAGTAGGCACTGATTCTAAAGGGACCGGTGCTGTACTTGAAGAAGAAACCGATAAACCAGAAGTGGGTGGTAAAAGTTGTGAGAATAGCACAGTATTTCCTTCTCTCCCTGTGGCTATTAGATGGCTCCGAGATAGCATCCTGCAGCAAAACCGGTCAATTCGACTTCAGGTTCTTGTAACTGGTTCCTTACATCTTGTGGGAGATGTGTTGAAATTAATCAAG

Protein sequence

AFEGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK

Homology

[Close]

BLAST of MS003153 vs. NCBI nr
Match: XP_022134298.1 (folylpolyglutamate synthase isoform X1 [Momordica charantia])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 538

Query: 544 K 545
           K
Sbjct: 544 K 538

[Close]

BLAST of MS003153 vs. NCBI nr
Match: XP_022134300.1 (folylpolyglutamate synthase isoform X2 [Momordica charantia])

HSP 1 Score: 1063.1 bits (2748), Expect = 7.8e-307
Identity = 531/541 (98.15%), Postives = 534/541 (98.71%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+K GTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEK-GTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 537

Query: 544 K 545
           K
Sbjct: 544 K 537

[Close]

BLAST of MS003153 vs. NCBI nr
Match: XP_022134301.1 (folylpolyglutamate synthase isoform X3 [Momordica charantia])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.3e-303
Identity = 526/541 (97.23%), Postives = 529/541 (97.78%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS      FIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS------FIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 532

Query: 544 K 545
           K
Sbjct: 544 K 532

[Close]

BLAST of MS003153 vs. NCBI nr
Match: XP_004142354.1 (folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein Csa_008061 [Cucumis sativus])

HSP 1 Score: 925.2 bits (2390), Expect = 2.5e-265
Identity = 465/544 (85.48%), Postives = 497/544 (91.36%), Query Frame = 0


Query: 3   EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
           EGN T+K SQ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEE M QMKVIH
Sbjct: 18  EGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKVIH 77

Query: 63  VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
           VAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW CY
Sbjct: 78  VAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCY 137

Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
           NRLKE+TS+DIPMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 138 NRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 197

Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
           ASLGYDHMELLGNTLGEIASEKAGIFKRG PAFTV QPDEAM VL+DKASQLDVQLTVVP
Sbjct: 198 ASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTVVP 257

Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
           SLDPELLHG KLGLEGEHQYLNAGLA+ALSS WL R+GHS+LSY+EQSRSLPEQFIEGLT
Sbjct: 258 SLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEGLT 317

Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
           TASFEGRAQIVPDRY D+E P +LVFYLDGAHSPESMEACGRWFSA ++ NQ G LN+Q 
Sbjct: 318 TASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNHQS 377

Query: 363 EDN--GISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNC 422
           EDN  G SSNK LQ   NDEARK SV VLLFNCM+VRDPQLLLPPLIKTCANNG      
Sbjct: 378 EDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNG------ 437

Query: 423 VHFKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGA 482
           V+FKKALFVPNTSVF+KVGTH L E + Q+DLSWQF++RR+WE+  QCDK G+DSKGTGA
Sbjct: 438 VNFKKALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDK-GSDSKGTGA 497

Query: 483 VLEEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVL 542
           VLEEE++K EVGGK+C NSTVF SLP+AI+WLRDS+LQQNRS+RLQVLVTGSLHLVGDVL
Sbjct: 498 VLEEESEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVL 553

Query: 543 KLIK 545
           KLIK
Sbjct: 558 KLIK 553

[Close]

BLAST of MS003153 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 923.3 bits (2385), Expect = 9.6e-265
Identity = 460/542 (84.87%), Postives = 488/542 (90.04%), Query Frame = 0


Query: 3   EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
           EG G +KYS+TAYEEALD LSSLIV+RRRCDKSGDRFELMFEYVKMLDLEE M +MKVIH
Sbjct: 3   EGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKVIH 62

Query: 63  VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
           VAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWWCY
Sbjct: 63  VAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCY 122

Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
           NRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 123 NRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 182

Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
           ASLGYDH ELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKASQLDVQLTVVP
Sbjct: 183 ASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVP 242

Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
           SLDPELL GHKLGLEGEHQYLNA LAIALSS WL RTGHS LSY EQS SLPEQFIEGLT
Sbjct: 243 SLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEGLT 302

Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
           TASFEGRAQIVPDRY DIESP DLVFYLDGAHSPESM  C  WF A +K NQ G L++QL
Sbjct: 303 TASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQL 362

Query: 363 EDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVH 422
           +DNG SSNKLLQ   NDEARK SV VLLFNCMSVRDPQLLL PLIKTCANNG      VH
Sbjct: 363 DDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNG------VH 422

Query: 423 FKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVL 482
           FKKA+FVPNTSVF+KVGTH L EANG++DLSWQF++RRVWEN+IQ +K G DS+G G ++
Sbjct: 423 FKKAVFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-GGDSQGIGTMV 482

Query: 483 EEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKL 542
           EEE++KPEV GK CENS VF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKL
Sbjct: 483 EEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKL 537

Query: 543 IK 545
           IK
Sbjct: 543 IK 537

[Close]

BLAST of MS003153 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.5e-185
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0


Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
           +LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NAGLA++L+S WLQ+ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
           +VPD+Y +  + GDLVFYLDGAHSPESMEAC +WFS  +K DNQ G+  + +  +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
                  +  + +T   +LLFNCMSVRDP LLLP L   CA  G      V+FKKALFVP
Sbjct: 426 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 485

Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
           N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G                 
Sbjct: 486 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 545

Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
           E  G+S  NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of MS003153 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 5.3e-157
Identity = 298/533 (55.91%), Postives = 371/533 (69.61%), Query Frame = 0


Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
           LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
           VPD     E P ++V+YLDGAHSPESMEAC  WFS  +K NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
            + NQ     K S  +LLFNCMSVRDP LLLP L   C + G      V FK+A+FVPN 
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432

Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
           SV+++VG+   T    +++ +SWQF ++R+WE+L + +              +   K + 
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 491

Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
            GK  E S VF SLPVA+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of MS003153 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.7e-153
Identity = 291/559 (52.06%), Postives = 383/559 (68.52%), Query Frame = 0


Query: 13  TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTK 72
           ++Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
           GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGY 192
           K    + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV++ P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPE 252
           DHM++LGNTL +IA  KAGIFK  +PAFTV Q  EAM+VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR--SLPEQFIEGLTTAS 312
            L G  LGL G+HQ +NAGLA++LS  WLQRTG+ K  +  +S+   +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYID-------IESP-GDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGT 372
             GRAQ+V D   D       +E+P GDL+FYLDGAHSPESMEACGRWFS+ +    RG 
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV----RGD 440

Query: 373 LNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYF 432
            +     NG     +       +  + S  +LLFNCM VRDPQ+LLP L+ TCA++G   
Sbjct: 441 KSLSTAVNGY----MRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSG--- 500

Query: 433 SNCVHFKKALFVPNTSVFDKV--GTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTD- 492
               HF +ALFVP+ S ++KV  G  A+     + DL+WQF ++R+WE  IQ    G D 
Sbjct: 501 ---THFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDH 560

Query: 493 ---SKGTGAVLEEE---TDKPEVGGKS---CENSTVFPSLPVAIRWLRDSILQQNRSIRL 545
                G  A+   +    D P+ GG +     +S V PSLP+ I WLRD + ++N S++L
Sbjct: 561 TLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKL 620

[Close]

BLAST of MS003153 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.6e-84
Identity = 218/587 (37.14%), Postives = 300/587 (51.11%), Query Frame = 0


Query: 15  YEEALDALSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-EPMLQMKVIHVAGT 74
           Y++A+  L++L  N    +    K GD   + E M  Y+    L+ E + Q+ +IH+ GT
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101

Query: 75  KGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLK 134
           KGKGSTC FTE ILR+ G  TG F+SPHL+ +RER R++G  IS E F  +FW  Y+RL+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161

Query: 135 E--KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
           E    S  + MP  FRFL L+AF +F  E+VD+A+LEVG+GG +D TN++R P+VCGI S
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221

Query: 195 LGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSL 254
           LG DH  LLG+T+ +IA +K GIFK GVPAFTV+QPDE + VLRD+A Q+   L + P L
Sbjct: 222 LGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPL 281

Query: 255 DPELLHGH--KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR-SLPEQ----- 314
                 G    LGLEGEHQ  NA LA+ ++  WLQ+ G+     ++ SR SLP Q     
Sbjct: 282 QALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAP 341

Query: 315 -------FIEGLTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAV 374
                    +GL    + GR Q++          G L +YLDGAH+  SM+AC RWF   
Sbjct: 342 VFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFRQA 401

Query: 375 MKDNQRGTLNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKT 434
           +   +R        D+G                 + V VLLFN    RD   LL  L++ 
Sbjct: 402 LHRCER-------PDSG-----------------SEVRVLLFNSTGDRDSAALL-KLLQP 461

Query: 435 CANNGKYFSNCVHFKKALFVPNTSVF---DKVGTHALTEANGQI---DLSWQFNIRRVWE 494
           C            F  A+F PN +     D       T    Q+    L+ Q +  R+ E
Sbjct: 462 C-----------QFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHE 521

Query: 495 NLIQCD---KVGTDSKGTGAVLEEETDKPEVGGKSCENSTVFPSLPVAIRWL---RDSIL 545
             +  D     G +  G  ++L      P        +S VF  +  A++W+   RD + 
Sbjct: 522 EQVSPDPWSTPGQEQDGPASLLL----APHPPHTHSASSLVFSCISHALQWITQGRDPLF 580

[Close]

BLAST of MS003153 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 310.8 bits (795), Expect = 3.0e-83
Identity = 211/586 (36.01%), Postives = 295/586 (50.34%), Query Frame = 0


Query: 15  YEEALDALSSLIVN--------RRRCDKSGDRFELMFEYVKMLDLE-EPMLQMKVIHVAG 74
           Y++A+  L++L  N        R+R D    + E M  Y+    L+ E + ++ +IHV G
Sbjct: 45  YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104

Query: 75  TKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRL 134
           TKGKGSTC FTE ILRN G  TG F+SPH++ +RER R++G  IS E F  +FW  YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164

Query: 135 KE-KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
           +E K    + MP  FRFL L+AF +F  E+VD+A++EVG+GG FD TN++R P+VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224

Query: 195 LGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSL 254
           LG DH  LLG+T+ +IA +K GIFK GVPAFTVVQP+  + VLRD+A Q+   L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284

Query: 255 D--PELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR-----SLP--- 314
           +   E+     LGLEG HQ  NA LA+ L+  WL+R  H  +  ++ SR      LP   
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344

Query: 315 -----EQFIEGLTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAV 374
                     GL    + GR QI+          G L +YLDGAH+  S++AC  W+   
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQIL--------QRGPLTWYLDGAHTTSSVQACVHWYRQS 404

Query: 375 MKDNQRGTLNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKT 434
           ++ ++R        D G                 + V +LLFN    RD   LL  L++ 
Sbjct: 405 LERSKR-------TDGG-----------------SEVHILLFNSTGDRDSAALL-KLLQP 464

Query: 435 CANNGKYFSNCVHFKKALFVPNTSVFDKVGTHALTEANGQID--LSWQFNIRRVWENLIQ 494
           C            F  A+F PN +    +G          +D  L      ++ W  L +
Sbjct: 465 C-----------QFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAE 524

Query: 495 -------CDKVGTDSKGTGAVLEEETDKPEVGGKSCENSTVFPSLPVAIRWL---RDSIL 544
                      G D  G G++L      P     +  +S VF  +  A+ W+   RD I 
Sbjct: 525 KQASSNLWSSCGPDPAGPGSLLL----APHPPQPTRTSSLVFSCISHALLWISQGRDPIF 581

[Close]

BLAST of MS003153 vs. ExPASy TrEMBL
Match: A0A6J1C1M5 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.3e-309
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 538

Query: 544 K 545
           K
Sbjct: 544 K 538

[Close]

BLAST of MS003153 vs. ExPASy TrEMBL
Match: A0A6J1BXX6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.8e-307
Identity = 531/541 (98.15%), Postives = 534/541 (98.71%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+K GTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEK-GTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 537

Query: 544 K 545
           K
Sbjct: 544 K 537

[Close]

BLAST of MS003153 vs. ExPASy TrEMBL
Match: A0A6J1BXH6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 526/541 (97.23%), Postives = 529/541 (97.78%), Query Frame = 0


Query: 4   GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
           GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4   GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63

Query: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
           AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64  AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123

Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
           RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183

Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
           SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243

Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
           LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS      FIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS------FIEGLTT 303

Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
           ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363

Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
           DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG      VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423

Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
           KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483

Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
           EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 532

Query: 544 K 545
           K
Sbjct: 544 K 532

[Close]

BLAST of MS003153 vs. ExPASy TrEMBL
Match: A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 465/544 (85.48%), Postives = 497/544 (91.36%), Query Frame = 0


Query: 3   EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
           EGN T+K SQ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEE M QMKVIH
Sbjct: 18  EGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKVIH 77

Query: 63  VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
           VAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW CY
Sbjct: 78  VAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCY 137

Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
           NRLKE+TS+DIPMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 138 NRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 197

Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
           ASLGYDHMELLGNTLGEIASEKAGIFKRG PAFTV QPDEAM VL+DKASQLDVQLTVVP
Sbjct: 198 ASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTVVP 257

Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
           SLDPELLHG KLGLEGEHQYLNAGLA+ALSS WL R+GHS+LSY+EQSRSLPEQFIEGLT
Sbjct: 258 SLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEGLT 317

Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
           TASFEGRAQIVPDRY D+E P +LVFYLDGAHSPESMEACGRWFSA ++ NQ G LN+Q 
Sbjct: 318 TASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNHQS 377

Query: 363 EDN--GISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNC 422
           EDN  G SSNK LQ   NDEARK SV VLLFNCM+VRDPQLLLPPLIKTCANNG      
Sbjct: 378 EDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNG------ 437

Query: 423 VHFKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGA 482
           V+FKKALFVPNTSVF+KVGTH L E + Q+DLSWQF++RR+WE+  QCDK G+DSKGTGA
Sbjct: 438 VNFKKALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDK-GSDSKGTGA 497

Query: 483 VLEEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVL 542
           VLEEE++K EVGGK+C NSTVF SLP+AI+WLRDS+LQQNRS+RLQVLVTGSLHLVGDVL
Sbjct: 498 VLEEESEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVL 553

Query: 543 KLIK 545
           KLIK
Sbjct: 558 KLIK 553

[Close]

BLAST of MS003153 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.0e-264
Identity = 460/542 (84.87%), Postives = 487/542 (89.85%), Query Frame = 0


Query: 3   EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
           EG G +KYS+TAYEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLEE M +MKVIH
Sbjct: 3   EGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKVIH 62

Query: 63  VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
           VAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWWCY
Sbjct: 63  VAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCY 122

Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
           NRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 123 NRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 182

Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
           ASLGYDH ELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKASQLDVQLTVVP
Sbjct: 183 ASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVP 242

Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
           SLDPELL GHKLGLEGEHQYLNA LAIALSS WL RTG S LSY EQS SLPEQFIEGLT
Sbjct: 243 SLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEGLT 302

Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
           TA FEGRAQIVPDRY DIE+P DLVFYLDGAHSPESM  C  WF A +K NQ G LN+QL
Sbjct: 303 TARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNHQL 362

Query: 363 EDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVH 422
           +DNG SSNKLLQ   NDEARK SV VLLFNCMSVRDPQLLL PLIKTCANNG      VH
Sbjct: 363 DDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNG------VH 422

Query: 423 FKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVL 482
           FKKALFVPNTS+F+KVGTH L EANG++DLSWQF++RRVWEN+IQ +K G DS+G GA++
Sbjct: 423 FKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-GGDSQGIGAMV 482

Query: 483 EEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKL 542
           EEE++KPEV GKSCENS VF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKL
Sbjct: 483 EEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKL 537

Query: 543 IK 545
           IK
Sbjct: 543 IK 537

[Close]

BLAST of MS003153 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-186
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0


Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
           +LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NAGLA++L+S WLQ+ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
           +VPD+Y +  + GDLVFYLDGAHSPESMEAC +WFS  +K DNQ G+  + +  +  SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
                  +  + +T   +LLFNCMSVRDP LLLP L   CA  G      V+FKKALFVP
Sbjct: 426 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 485

Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
           N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G                 
Sbjct: 486 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 545

Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
           E  G+S  NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

[Close]

BLAST of MS003153 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-186
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0


Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
           +IPMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
           +LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV  L   LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
            KLGL+GEHQY+NAGLA++L+S WLQ+ G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
           +VPD+Y +  + GDLVFYLDGAHSPESMEAC +WFS  +K DNQ G+  + +  +  SS+
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367

Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
                  +  + +T   +LLFNCMSVRDP LLLP L   CA  G      V+FKKALFVP
Sbjct: 368 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 427

Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
           N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G                 
Sbjct: 428 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 487

Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
           E  G+S  NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

[Close]

BLAST of MS003153 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 555.8 bits (1431), Expect = 3.8e-158
Identity = 298/533 (55.91%), Postives = 371/533 (69.61%), Query Frame = 0


Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
           LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
           VPD     E P ++V+YLDGAHSPESMEAC  WFS  +K NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
            + NQ     K S  +LLFNCMSVRDP LLLP L   C + G      V FK+A+FVPN 
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432

Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
           SV+++VG+   T    +++ +SWQF ++R+WE+L + +              +   K + 
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 491

Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
            GK  E S VF SLPVA+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 491

[Close]

BLAST of MS003153 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-156
Identity = 297/533 (55.72%), Postives = 370/533 (69.42%), Query Frame = 0


Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
           +PMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATN ++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
           LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV  LD       
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNAGLA+AL S +L+  G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
           VPD     E P ++V+YLDGAHSPESMEAC  WFS  +K NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
            + NQ     K S  +LLFNCMSVRDP LLLP L   C + G      V FK+A+FVPN 
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432

Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
           SV+++VG+   T    +++ +SWQF ++R+WE+L + +              +   K + 
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 490

Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
            GK  E S VF SLPVA+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 490

[Close]

BLAST of MS003153 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 543.5 bits (1399), Expect = 1.9e-154
Identity = 291/559 (52.06%), Postives = 383/559 (68.52%), Query Frame = 0


Query: 13  TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTK 72
           ++Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
           GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGY 192
           K    + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV++ P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPE 252
           DHM++LGNTL +IA  KAGIFK  +PAFTV Q  EAM+VL+  A+ L+V L VV  L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR--SLPEQFIEGLTTAS 312
            L G  LGL G+HQ +NAGLA++LS  WLQRTG+ K  +  +S+   +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYID-------IESP-GDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGT 372
             GRAQ+V D   D       +E+P GDL+FYLDGAHSPESMEACGRWFS+ +    RG 
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV----RGD 440

Query: 373 LNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYF 432
            +     NG     +       +  + S  +LLFNCM VRDPQ+LLP L+ TCA++G   
Sbjct: 441 KSLSTAVNGY----MRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSG--- 500

Query: 433 SNCVHFKKALFVPNTSVFDKV--GTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTD- 492
               HF +ALFVP+ S ++KV  G  A+     + DL+WQF ++R+WE  IQ    G D 
Sbjct: 501 ---THFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDH 560

Query: 493 ---SKGTGAVLEEE---TDKPEVGGKS---CENSTVFPSLPVAIRWLRDSILQQNRSIRL 545
                G  A+   +    D P+ GG +     +S V PSLP+ I WLRD + ++N S++L
Sbjct: 561 TLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKL 620

The following BLAST results are available for this feature:

BLAST of MS003153 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134298.1	1.1e-308	98.34	folylpolyglutamate synthase isoform X1 [Momordica charantia]	[more]
XP_022134300.1	7.8e-307	98.15	folylpolyglutamate synthase isoform X2 [Momordica charantia]	[more]
XP_022134301.1	2.3e-303	97.23	folylpolyglutamate synthase isoform X3 [Momordica charantia]	[more]
XP_004142354.1	2.5e-265	85.48	folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein C...	[more]
XP_023533507.1	9.6e-265	84.87	folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS003153 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4K2A1	3.5e-185	62.62	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1	[more]
Q8W035	5.3e-157	55.91	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1	[more]
F4J2K2	2.7e-153	52.06	Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1	[more]
A6H751	4.6e-84	37.14	Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV...	[more]
P48760	3.0e-83	36.01	Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1...	[more]

BLAST of MS003153 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1M5	5.3e-309	98.34	Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ...	[more]
A0A6J1BXX6	3.8e-307	98.15	Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ...	[more]
A0A6J1BXH6	1.1e-303	97.23	Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ...	[more]
A0A0A0KTX7	1.2e-265	85.48	Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1	[more]
A0A6J1JQH1	1.0e-264	84.87	Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=...	[more]

BLAST of MS003153 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05980.1	2.5e-186	62.62	DHFS-FPGS homolog B	[more]
AT5G05980.2	2.5e-186	62.62	DHFS-FPGS homolog B	[more]
AT3G55630.3	3.8e-158	55.91	DHFS-FPGS homolog D	[more]
AT3G55630.2	2.1e-156	55.72	DHFS-FPGS homolog D	[more]
AT3G10160.1	1.9e-154	52.06	DHFS-FPGS homolog C	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 5..290 e-value: 6.1E-90 score: 303.2
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 26..276
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 63..237 e-value: 1.4E-5 score: 25.1
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 308..544 e-value: 2.9E-8 score: 36.0
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 308..543
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 51..354 e-value: 1.5E-88 score: 295.8
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 10..544
IPR023600	Folylpolyglutamate synthase, eukaryota	PIRSF	PIRSF038895	FPGS	coord: 2..544 e-value: 5.7E-148 score: 491.4
None	No IPR available	PANTHER	PTHR11136:SF10	FOLYLPOLYGLUTAMATE SYNTHASE	coord: 10..544
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 159..174
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 61..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003153.1	MS003153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity