Homology
BLAST of MS003153 vs. NCBI nr
Match:
XP_022134298.1 (folylpolyglutamate synthase isoform X1 [Momordica charantia])
HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 538
Query: 544 K 545
K
Sbjct: 544 K 538
BLAST of MS003153 vs. NCBI nr
Match:
XP_022134300.1 (folylpolyglutamate synthase isoform X2 [Momordica charantia])
HSP 1 Score: 1063.1 bits (2748), Expect = 7.8e-307
Identity = 531/541 (98.15%), Postives = 534/541 (98.71%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+K GTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEK-GTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 537
Query: 544 K 545
K
Sbjct: 544 K 537
BLAST of MS003153 vs. NCBI nr
Match:
XP_022134301.1 (folylpolyglutamate synthase isoform X3 [Momordica charantia])
HSP 1 Score: 1051.6 bits (2718), Expect = 2.3e-303
Identity = 526/541 (97.23%), Postives = 529/541 (97.78%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS FIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS------FIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 532
Query: 544 K 545
K
Sbjct: 544 K 532
BLAST of MS003153 vs. NCBI nr
Match:
XP_004142354.1 (folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein Csa_008061 [Cucumis sativus])
HSP 1 Score: 925.2 bits (2390), Expect = 2.5e-265
Identity = 465/544 (85.48%), Postives = 497/544 (91.36%), Query Frame = 0
Query: 3 EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
EGN T+K SQ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEE M QMKVIH
Sbjct: 18 EGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKVIH 77
Query: 63 VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
VAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW CY
Sbjct: 78 VAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCY 137
Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
NRLKE+TS+DIPMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 138 NRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 197
Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
ASLGYDHMELLGNTLGEIASEKAGIFKRG PAFTV QPDEAM VL+DKASQLDVQLTVVP
Sbjct: 198 ASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTVVP 257
Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
SLDPELLHG KLGLEGEHQYLNAGLA+ALSS WL R+GHS+LSY+EQSRSLPEQFIEGLT
Sbjct: 258 SLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEGLT 317
Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
TASFEGRAQIVPDRY D+E P +LVFYLDGAHSPESMEACGRWFSA ++ NQ G LN+Q
Sbjct: 318 TASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNHQS 377
Query: 363 EDN--GISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNC 422
EDN G SSNK LQ NDEARK SV VLLFNCM+VRDPQLLLPPLIKTCANNG
Sbjct: 378 EDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNG------ 437
Query: 423 VHFKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGA 482
V+FKKALFVPNTSVF+KVGTH L E + Q+DLSWQF++RR+WE+ QCDK G+DSKGTGA
Sbjct: 438 VNFKKALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDK-GSDSKGTGA 497
Query: 483 VLEEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVL 542
VLEEE++K EVGGK+C NSTVF SLP+AI+WLRDS+LQQNRS+RLQVLVTGSLHLVGDVL
Sbjct: 498 VLEEESEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVL 553
Query: 543 KLIK 545
KLIK
Sbjct: 558 KLIK 553
BLAST of MS003153 vs. NCBI nr
Match:
XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 923.3 bits (2385), Expect = 9.6e-265
Identity = 460/542 (84.87%), Postives = 488/542 (90.04%), Query Frame = 0
Query: 3 EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
EG G +KYS+TAYEEALD LSSLIV+RRRCDKSGDRFELMFEYVKMLDLEE M +MKVIH
Sbjct: 3 EGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKVIH 62
Query: 63 VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
VAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWWCY
Sbjct: 63 VAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCY 122
Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
NRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 123 NRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 182
Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
ASLGYDH ELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKASQLDVQLTVVP
Sbjct: 183 ASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTVVP 242
Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
SLDPELL GHKLGLEGEHQYLNA LAIALSS WL RTGHS LSY EQS SLPEQFIEGLT
Sbjct: 243 SLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEGLT 302
Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
TASFEGRAQIVPDRY DIESP DLVFYLDGAHSPESM C WF A +K NQ G L++QL
Sbjct: 303 TASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQL 362
Query: 363 EDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVH 422
+DNG SSNKLLQ NDEARK SV VLLFNCMSVRDPQLLL PLIKTCANNG VH
Sbjct: 363 DDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNG------VH 422
Query: 423 FKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVL 482
FKKA+FVPNTSVF+KVGTH L EANG++DLSWQF++RRVWEN+IQ +K G DS+G G ++
Sbjct: 423 FKKAVFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-GGDSQGIGTMV 482
Query: 483 EEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKL 542
EEE++KPEV GK CENS VF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKL
Sbjct: 483 EEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKL 537
Query: 543 IK 545
IK
Sbjct: 543 IK 537
BLAST of MS003153 vs. ExPASy Swiss-Prot
Match:
F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)
HSP 1 Score: 649.4 bits (1674), Expect = 3.5e-185
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0
Query: 14 AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
+YEEAL ALSSLI R R DKS GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 66 SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125
Query: 74 TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185
Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
+IPMP FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245
Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
+LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV L LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305
Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
KLGL+GEHQY+NAGLA++L+S WLQ+ G ++ Q LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365
Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
+VPD+Y + + GDLVFYLDGAHSPESMEAC +WFS +K DNQ G+ + + + SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425
Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
+ + +T +LLFNCMSVRDP LLLP L CA G V+FKKALFVP
Sbjct: 426 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 485
Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G
Sbjct: 486 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 545
Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
E G+S NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570
BLAST of MS003153 vs. ExPASy Swiss-Prot
Match:
Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)
HSP 1 Score: 555.8 bits (1431), Expect = 5.3e-157
Identity = 298/533 (55.91%), Postives = 371/533 (69.61%), Query Frame = 0
Query: 15 YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
Y+ LDALSSLI R R + RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13 YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72
Query: 75 CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
CTF ESILR G TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73 CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132
Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
+PMP F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192
Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV LD
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252
Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
+LGL+GEHQYLNAGLA+AL S +L+ G + ++Q+ LPE+FI GL+ A GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312
Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
VPD E P ++V+YLDGAHSPESMEAC WFS +K NQ
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372
Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
+ NQ K S +LLFNCMSVRDP LLLP L C + G V FK+A+FVPN
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432
Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
SV+++VG+ T +++ +SWQF ++R+WE+L + + + K +
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 491
Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
GK E S VF SLPVA+ WLRD+ +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 491
BLAST of MS003153 vs. ExPASy Swiss-Prot
Match:
F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)
HSP 1 Score: 543.5 bits (1399), Expect = 2.7e-153
Identity = 291/559 (52.06%), Postives = 383/559 (68.52%), Query Frame = 0
Query: 13 TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTK 72
++Y++A++ALS+LI R R D+ + D+ E + Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81 SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140
Query: 73 GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+ LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200
Query: 133 KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGY 192
K + MP LF+FL +LAFKIF E+VDVA++EVGLGG D+TNV++ P+VCGIASLG
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260
Query: 193 DHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPE 252
DHM++LGNTL +IA KAGIFK +PAFTV Q EAM+VL+ A+ L+V L VV L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320
Query: 253 LLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR--SLPEQFIEGLTTAS 312
L G LGL G+HQ +NAGLA++LS WLQRTG+ K + +S+ +P F GL TA
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380
Query: 313 FEGRAQIVPDRYID-------IESP-GDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGT 372
GRAQ+V D D +E+P GDL+FYLDGAHSPESMEACGRWFS+ + RG
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV----RGD 440
Query: 373 LNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYF 432
+ NG + + + S +LLFNCM VRDPQ+LLP L+ TCA++G
Sbjct: 441 KSLSTAVNGY----MRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSG--- 500
Query: 433 SNCVHFKKALFVPNTSVFDKV--GTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTD- 492
HF +ALFVP+ S ++KV G A+ + DL+WQF ++R+WE IQ G D
Sbjct: 501 ---THFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDH 560
Query: 493 ---SKGTGAVLEEE---TDKPEVGGKS---CENSTVFPSLPVAIRWLRDSILQQNRSIRL 545
G A+ + D P+ GG + +S V PSLP+ I WLRD + ++N S++L
Sbjct: 561 TLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKL 620
BLAST of MS003153 vs. ExPASy Swiss-Prot
Match:
A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)
HSP 1 Score: 313.5 bits (802), Expect = 4.6e-84
Identity = 218/587 (37.14%), Postives = 300/587 (51.11%), Query Frame = 0
Query: 15 YEEALDALSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-EPMLQMKVIHVAGT 74
Y++A+ L++L N + K GD + E M Y+ L+ E + Q+ +IH+ GT
Sbjct: 42 YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101
Query: 75 KGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLK 134
KGKGSTC FTE ILR+ G TG F+SPHL+ +RER R++G IS E F +FW Y+RL+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161
Query: 135 E--KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
E S + MP FRFL L+AF +F E+VD+A+LEVG+GG +D TN++R P+VCGI S
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221
Query: 195 LGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSL 254
LG DH LLG+T+ +IA +K GIFK GVPAFTV+QPDE + VLRD+A Q+ L + P L
Sbjct: 222 LGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPL 281
Query: 255 DPELLHGH--KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR-SLPEQ----- 314
G LGLEGEHQ NA LA+ ++ WLQ+ G+ ++ SR SLP Q
Sbjct: 282 QALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAP 341
Query: 315 -------FIEGLTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAV 374
+GL + GR Q++ G L +YLDGAH+ SM+AC RWF
Sbjct: 342 VFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFRQA 401
Query: 375 MKDNQRGTLNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKT 434
+ +R D+G + V VLLFN RD LL L++
Sbjct: 402 LHRCER-------PDSG-----------------SEVRVLLFNSTGDRDSAALL-KLLQP 461
Query: 435 CANNGKYFSNCVHFKKALFVPNTSVF---DKVGTHALTEANGQI---DLSWQFNIRRVWE 494
C F A+F PN + D T Q+ L+ Q + R+ E
Sbjct: 462 C-----------QFDYAIFCPNLAEMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHE 521
Query: 495 NLIQCD---KVGTDSKGTGAVLEEETDKPEVGGKSCENSTVFPSLPVAIRWL---RDSIL 545
+ D G + G ++L P +S VF + A++W+ RD +
Sbjct: 522 EQVSPDPWSTPGQEQDGPASLLL----APHPPHTHSASSLVFSCISHALQWITQGRDPLF 580
BLAST of MS003153 vs. ExPASy Swiss-Prot
Match:
P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)
HSP 1 Score: 310.8 bits (795), Expect = 3.0e-83
Identity = 211/586 (36.01%), Postives = 295/586 (50.34%), Query Frame = 0
Query: 15 YEEALDALSSLIVN--------RRRCDKSGDRFELMFEYVKMLDLE-EPMLQMKVIHVAG 74
Y++A+ L++L N R+R D + E M Y+ L+ E + ++ +IHV G
Sbjct: 45 YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104
Query: 75 TKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRL 134
TKGKGSTC FTE ILRN G TG F+SPH++ +RER R++G IS E F +FW YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164
Query: 135 KE-KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIAS 194
+E K + MP FRFL L+AF +F E+VD+A++EVG+GG FD TN++R P+VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224
Query: 195 LGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSL 254
LG DH LLG+T+ +IA +K GIFK GVPAFTVVQP+ + VLRD+A Q+ L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284
Query: 255 D--PELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR-----SLP--- 314
+ E+ LGLEG HQ NA LA+ L+ WL+R H + ++ SR LP
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344
Query: 315 -----EQFIEGLTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAV 374
GL + GR QI+ G L +YLDGAH+ S++AC W+
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQIL--------QRGPLTWYLDGAHTTSSVQACVHWYRQS 404
Query: 375 MKDNQRGTLNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKT 434
++ ++R D G + V +LLFN RD LL L++
Sbjct: 405 LERSKR-------TDGG-----------------SEVHILLFNSTGDRDSAALL-KLLQP 464
Query: 435 CANNGKYFSNCVHFKKALFVPNTSVFDKVGTHALTEANGQID--LSWQFNIRRVWENLIQ 494
C F A+F PN + +G +D L ++ W L +
Sbjct: 465 C-----------QFDYAVFCPNVTEVSSIGNADQQNFTVTLDQVLLRCLQHQQHWNGLAE 524
Query: 495 -------CDKVGTDSKGTGAVLEEETDKPEVGGKSCENSTVFPSLPVAIRWL---RDSIL 544
G D G G++L P + +S VF + A+ W+ RD I
Sbjct: 525 KQASSNLWSSCGPDPAGPGSLLL----APHPPQPTRTSSLVFSCISHALLWISQGRDPIF 581
BLAST of MS003153 vs. ExPASy TrEMBL
Match:
A0A6J1C1M5 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)
HSP 1 Score: 1069.3 bits (2764), Expect = 5.3e-309
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 538
Query: 544 K 545
K
Sbjct: 544 K 538
BLAST of MS003153 vs. ExPASy TrEMBL
Match:
A0A6J1BXX6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)
HSP 1 Score: 1063.1 bits (2748), Expect = 3.8e-307
Identity = 531/541 (98.15%), Postives = 534/541 (98.71%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+K GTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEK-GTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 537
Query: 544 K 545
K
Sbjct: 544 K 537
BLAST of MS003153 vs. ExPASy TrEMBL
Match:
A0A6J1BXH6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)
HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 526/541 (97.23%), Postives = 529/541 (97.78%), Query Frame = 0
Query: 4 GNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
GNGT+KYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV
Sbjct: 4 GNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIHV 63
Query: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN
Sbjct: 64 AGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYN 123
Query: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA
Sbjct: 124 RLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIA 183
Query: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPS 243
SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKAS+LDVQLTVVPS
Sbjct: 184 SLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTVVPS 243
Query: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTT 303
LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS FIEGLTT
Sbjct: 244 LDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQS------FIEGLTT 303
Query: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE
Sbjct: 304 ASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLE 363
Query: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHF 423
DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG VHF
Sbjct: 364 DNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNG------VHF 423
Query: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLE 483
KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQC+KVGTDSKGTGAVLE
Sbjct: 424 KKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKVGTDSKGTGAVLE 483
Query: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 543
EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI
Sbjct: 484 EETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLI 532
Query: 544 K 545
K
Sbjct: 544 K 532
BLAST of MS003153 vs. ExPASy TrEMBL
Match:
A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)
HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 465/544 (85.48%), Postives = 497/544 (91.36%), Query Frame = 0
Query: 3 EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
EGN T+K SQ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEE M QMKVIH
Sbjct: 18 EGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKVIH 77
Query: 63 VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
VAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW CY
Sbjct: 78 VAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWCCY 137
Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
NRLKE+TS+DIPMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 138 NRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 197
Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
ASLGYDHMELLGNTLGEIASEKAGIFKRG PAFTV QPDEAM VL+DKASQLDVQLTVVP
Sbjct: 198 ASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTVVP 257
Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
SLDPELLHG KLGLEGEHQYLNAGLA+ALSS WL R+GHS+LSY+EQSRSLPEQFIEGLT
Sbjct: 258 SLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEGLT 317
Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
TASFEGRAQIVPDRY D+E P +LVFYLDGAHSPESMEACGRWFSA ++ NQ G LN+Q
Sbjct: 318 TASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNHQS 377
Query: 363 EDN--GISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNC 422
EDN G SSNK LQ NDEARK SV VLLFNCM+VRDPQLLLPPLIKTCANNG
Sbjct: 378 EDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNG------ 437
Query: 423 VHFKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGA 482
V+FKKALFVPNTSVF+KVGTH L E + Q+DLSWQF++RR+WE+ QCDK G+DSKGTGA
Sbjct: 438 VNFKKALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDK-GSDSKGTGA 497
Query: 483 VLEEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVL 542
VLEEE++K EVGGK+C NSTVF SLP+AI+WLRDS+LQQNRS+RLQVLVTGSLHLVGDVL
Sbjct: 498 VLEEESEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVL 553
Query: 543 KLIK 545
KLIK
Sbjct: 558 KLIK 553
BLAST of MS003153 vs. ExPASy TrEMBL
Match:
A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)
HSP 1 Score: 922.2 bits (2382), Expect = 1.0e-264
Identity = 460/542 (84.87%), Postives = 487/542 (89.85%), Query Frame = 0
Query: 3 EGNGTMKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKVIH 62
EG G +KYS+TAYEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLEE M +MKVIH
Sbjct: 3 EGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKVIH 62
Query: 63 VAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCY 122
VAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWWCY
Sbjct: 63 VAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCY 122
Query: 123 NRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGI 182
NRLKEKTS+D+PMP LFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVV+APIVCGI
Sbjct: 123 NRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVCGI 182
Query: 183 ASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVP 242
ASLGYDH ELLGNTLG IASEKAGIFKRG PAFTV QPDEAM+VLRDKASQLDVQLTVVP
Sbjct: 183 ASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTVVP 242
Query: 243 SLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLT 302
SLDPELL GHKLGLEGEHQYLNA LAIALSS WL RTG S LSY EQS SLPEQFIEGLT
Sbjct: 243 SLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEGLT 302
Query: 303 TASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQL 362
TA FEGRAQIVPDRY DIE+P DLVFYLDGAHSPESM C WF A +K NQ G LN+QL
Sbjct: 303 TARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNHQL 362
Query: 363 EDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVH 422
+DNG SSNKLLQ NDEARK SV VLLFNCMSVRDPQLLL PLIKTCANNG VH
Sbjct: 363 DDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNG------VH 422
Query: 423 FKKALFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVL 482
FKKALFVPNTS+F+KVGTH L EANG++DLSWQF++RRVWEN+IQ +K G DS+G GA++
Sbjct: 423 FKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEK-GGDSQGIGAMV 482
Query: 483 EEETDKPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKL 542
EEE++KPEV GKSCENS VF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKL
Sbjct: 483 EEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKL 537
Query: 543 IK 545
IK
Sbjct: 543 IK 537
BLAST of MS003153 vs. TAIR 10
Match:
AT5G05980.1 (DHFS-FPGS homolog B )
HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-186
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0
Query: 14 AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
+YEEAL ALSSLI R R DKS GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 66 SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125
Query: 74 TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185
Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
+IPMP FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245
Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
+LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV L LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305
Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
KLGL+GEHQY+NAGLA++L+S WLQ+ G ++ Q LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365
Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
+VPD+Y + + GDLVFYLDGAHSPESMEAC +WFS +K DNQ G+ + + + SS+
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425
Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
+ + +T +LLFNCMSVRDP LLLP L CA G V+FKKALFVP
Sbjct: 426 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 485
Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G
Sbjct: 486 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 545
Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
E G+S NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570
BLAST of MS003153 vs. TAIR 10
Match:
AT5G05980.2 (DHFS-FPGS homolog B )
HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-186
Identity = 335/535 (62.62%), Postives = 407/535 (76.07%), Query Frame = 0
Query: 14 AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGS 73
+YEEAL ALSSLI R R DKS GDRFEL+F+Y+K+LDLEE +L+M VIHVAGTKGKGS
Sbjct: 8 SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67
Query: 74 TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSK 133
TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T++
Sbjct: 68 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127
Query: 134 DIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHME 193
+IPMP FRFLALLAFKIF+ E+VD AILEVGLGG FDATN V+ P+VCGI+SLGYDHME
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187
Query: 194 LLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHG 253
+LG+TLG+IA EKAGIFK GVPAFTV QPDEAM VL +KAS+ +V L VV L LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247
Query: 254 HKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQ 313
KLGL+GEHQY+NAGLA++L+S WLQ+ G ++ Q LPE+FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307
Query: 314 IVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMK-DNQRGTLNYQLEDNGISSN 373
+VPD+Y + + GDLVFYLDGAHSPESMEAC +WFS +K DNQ G+ + + + SS+
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367
Query: 374 KLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVP 433
+ + +T +LLFNCMSVRDP LLLP L CA G V+FKKALFVP
Sbjct: 368 -------DKWSNETCEQILLFNCMSVRDPNLLLPHLKNMCAKYG------VNFKKALFVP 427
Query: 434 NTSVFDKVGTHA-LTEANGQIDLSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKP 493
N SV+ KVGT A L E + Q+DLSWQF +++VWE+L+Q ++ G
Sbjct: 428 NMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------- 487
Query: 494 EVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
E G+S NS VF SLP+AI+ LRD++ + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 EKDGESDGNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512
BLAST of MS003153 vs. TAIR 10
Match:
AT3G55630.3 (DHFS-FPGS homolog D )
HSP 1 Score: 555.8 bits (1431), Expect = 3.8e-158
Identity = 298/533 (55.91%), Postives = 371/533 (69.61%), Query Frame = 0
Query: 15 YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
Y+ LDALSSLI R R + RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13 YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72
Query: 75 CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
CTF ESILR G TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73 CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132
Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
+PMP F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192
Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV LD
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252
Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
+LGL+GEHQYLNAGLA+AL S +L+ G + ++Q+ LPE+FI GL+ A GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312
Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
VPD E P ++V+YLDGAHSPESMEAC WFS +K NQ
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372
Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
+ NQ K S +LLFNCMSVRDP LLLP L C + G V FK+A+FVPN
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432
Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
SV+++VG+ T +++ +SWQF ++R+WE+L + + + K +
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 491
Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
GK E S VF SLPVA+ WLRD+ +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 491
BLAST of MS003153 vs. TAIR 10
Match:
AT3G55630.2 (DHFS-FPGS homolog D )
HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-156
Identity = 297/533 (55.72%), Postives = 370/533 (69.42%), Query Frame = 0
Query: 15 YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTKGKGST 74
Y+ LDALSSLI R R + RF L+F Y+K+L+LE+ + QMK+IHVAGTKGKGST
Sbjct: 13 YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72
Query: 75 CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSKD 134
CTF ESILR G TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73 CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132
Query: 135 IPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGYDHMEL 194
+PMP F FLALLAFKIF+ EQVDV ILEVGLGG FDATN ++ P+VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192
Query: 195 LGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPELLHGH 254
LG TL EIA+EKAGIFK GVPAFTV QPDEAM VL +KAS+L+V L VV LD
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252
Query: 255 KLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEGLTTASFEGRAQI 314
+LGL+GEHQYLNAGLA+AL S +L+ G + ++Q+ LPE+FI GL+ A GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312
Query: 315 VPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNYQLEDNGISSNKL 374
VPD E P ++V+YLDGAHSPESMEAC WFS +K NQ
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372
Query: 375 LQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYFSNCVHFKKALFVPNT 434
+ NQ K S +LLFNCMSVRDP LLLP L C + G V FK+A+FVPN
Sbjct: 373 -ERNQ-----KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQG------VDFKRAVFVPNV 432
Query: 435 SVFDKVGTHALTEANGQID-LSWQFNIRRVWENLIQCDKVGTDSKGTGAVLEEETDKPEV 494
SV+++VG+ T +++ +SWQF ++R+WE+L + + + K +
Sbjct: 433 SVYNQVGSS--TNVGTRVESMSWQFGLQRIWESLARGE-------------AKSNSKSDS 490
Query: 495 GGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 545
GK E S VF SLPVA+ WLRD+ +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 KGKEEEKSFVFSSLPVAVDWLRDN-ARQSKQVRFQVLVTGSLHLVGDLLRFIK 490
BLAST of MS003153 vs. TAIR 10
Match:
AT3G10160.1 (DHFS-FPGS homolog C )
HSP 1 Score: 543.5 bits (1399), Expect = 1.9e-154
Identity = 291/559 (52.06%), Postives = 383/559 (68.52%), Query Frame = 0
Query: 13 TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEEPMLQMKVIHVAGTK 72
++Y++A++ALS+LI R R D+ + D+ E + Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81 SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140
Query: 73 GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+ LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200
Query: 133 KTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVCGIASLGY 192
K + MP LF+FL +LAFKIF E+VDVA++EVGLGG D+TNV++ P+VCGIASLG
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260
Query: 193 DHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASQLDVQLTVVPSLDPE 252
DHM++LGNTL +IA KAGIFK +PAFTV Q EAM+VL+ A+ L+V L VV L+P+
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320
Query: 253 LLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSR--SLPEQFIEGLTTAS 312
L G LGL G+HQ +NAGLA++LS WLQRTG+ K + +S+ +P F GL TA
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380
Query: 313 FEGRAQIVPDRYID-------IESP-GDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGT 372
GRAQ+V D D +E+P GDL+FYLDGAHSPESMEACGRWFS+ + RG
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV----RGD 440
Query: 373 LNYQLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGKYF 432
+ NG + + + S +LLFNCM VRDPQ+LLP L+ TCA++G
Sbjct: 441 KSLSTAVNGY----MRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSG--- 500
Query: 433 SNCVHFKKALFVPNTSVFDKV--GTHALTEANGQIDLSWQFNIRRVWENLIQCDKVGTD- 492
HF +ALFVP+ S ++KV G A+ + DL+WQF ++R+WE IQ G D
Sbjct: 501 ---THFSRALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDH 560
Query: 493 ---SKGTGAVLEEE---TDKPEVGGKS---CENSTVFPSLPVAIRWLRDSILQQNRSIRL 545
G A+ + D P+ GG + +S V PSLP+ I WLRD + ++N S++L
Sbjct: 561 TLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKL 620
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022134298.1 | 1.1e-308 | 98.34 | folylpolyglutamate synthase isoform X1 [Momordica charantia] | [more] |
XP_022134300.1 | 7.8e-307 | 98.15 | folylpolyglutamate synthase isoform X2 [Momordica charantia] | [more] |
XP_022134301.1 | 2.3e-303 | 97.23 | folylpolyglutamate synthase isoform X3 [Momordica charantia] | [more] |
XP_004142354.1 | 2.5e-265 | 85.48 | folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein C... | [more] |
XP_023533507.1 | 9.6e-265 | 84.87 | folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
F4K2A1 | 3.5e-185 | 62.62 | Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1 | [more] |
Q8W035 | 5.3e-157 | 55.91 | Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1 | [more] |
F4J2K2 | 2.7e-153 | 52.06 | Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1 | [more] |
A6H751 | 4.6e-84 | 37.14 | Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV... | [more] |
P48760 | 3.0e-83 | 36.01 | Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C1M5 | 5.3e-309 | 98.34 | Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ... | [more] |
A0A6J1BXX6 | 3.8e-307 | 98.15 | Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ... | [more] |
A0A6J1BXH6 | 1.1e-303 | 97.23 | Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 ... | [more] |
A0A0A0KTX7 | 1.2e-265 | 85.48 | Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1 | [more] |
A0A6J1JQH1 | 1.0e-264 | 84.87 | Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=... | [more] |