MS003143 (gene) Bitter gourd (TR) v1

Overview
NameMS003143
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionL-aspartate oxidase
Locationscaffold234: 707650 .. 711050 (+)
RNA-Seq ExpressionMS003143
SyntenyMS003143
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTTGTATACCTGTTGGAAGCTGCAAACTGTTCTGCAGGGCGGTGGAGTTGAAGGGGCAAGGTTTTAGAAGAGCCCAATGGGACGCTAGCATAGCTTTCAAAAGATTCTTTCAGGACGAGCTTTCACGGTATGGAATGCAAATATCGAGCCTCGGATGAACCTAATTATCAAAACAATTTTCTTTAGGTTTTCTGCATGCTTGCCTGGATCGCTTCAATTGTTTTTGTCGATATCATGTTTGGAAATAGGCTGCAGCTGAAATGTAGTTGGATATTTCACGTTTCCAGAACTAATATCAGAAGTGATCCACAAAATATGATGGGAATGGGATCTAGGAGTGACTTCTCTTTATACCACACTAAATTGTGACATTAAACCGAAGAACTATATACAAGGAAATTTTATTTTGTCATATCATTTCTTGTATTTAACTATGCCATTATATGTCGGAGAATAGCAGGTATATGCTTGTTGAATAAAATCACAAATTGTTGCATTTGTTGCGTCAAGAGATGCACATCAACATTTACAACTTGAGAACAATATTGGTTTTCTACTAATATAAAATGAACTTCGAGATGTGTATTTTCTTTTTTGTCTTATAAGTGAAAAAATGACATGGTAACAACATTGGTTTGCTACTAATATAAAATGAACTTCGGATATGTATTTTATTTTGTCTCACAAGTGAAGATGATATGATAAAATAGGATTCTTCGTACACAACAAAAAAAGTATTTAACTCCTATTGAGACATGTGAAGATAGTAGGAATATATTTTTGTCTATTACATTGGTAAGAGTGTAGAATCCTTCACTCTTGTACTATTCAACACCATGGACTTCATTTGACTTATATTGTTTGGCACAATCATCCAGATCATGTAGGTTGAATAAGTTCTCACATCTGCAAAGAAACAATATTTCCCAACCTACAATTAATGAGAAATGGAGAACTATTGCGACAGTCACTTCATCGTGCTTGAGTGACGGTTCAACAAAGTATTTTGATTTTGTTGTTATTGGTAGTGGAGTTGCTGGCCTCCGTTACGCTCTTGAAGTTGCAAAGCATGGATCCGTAGCCGTGATCACTAAGGCTGAGCCTCATGAGAGCAACACCAATTATGCTCAAGGTGGTGTTAGTGCCGTTCTTTGTCCTTCAGACTCTGTTGAAAGTCATGTGCAGGACACCATCGTAGCTGGGGCTCATCTGTGCGACGAGGAGACAGTTAGAGTATGTTTTTCAGCTTGCACTGTCCTACCTTTTTACTTTTGAAAGCTCATTATGCTCGGTTGTTCTGTTCTTTTCTAAGGTTCCCTATTTTATTTAGGTTGTATGTACAGAAGGGCCTGACAGAATCAAAGAGTTGATTGCTATGGGTGCGTCATTTGATCATGGGGAAGATGGTAACTTACACCTAGCAAGGGAAGGGGGGCACTCTCATCGTAGGATTGTTCATGCTGCTGATATGACTGGAAGGGAAATTGAGCGGGCTCTTTTGGAGGCGGTTGTTCGGGATCCCAACATTTTTGTGTTTGAGCACCATTTTGCAATTGACTTGCTAACTTCCGAGGTTTGCTTTTCCCTATTTCTTTCAACAGAATTTTTGTTTTAATATGCTTTCCTTGTTTCTGCCAATTTTAGTGACTGTATTAGAATAAACTATGAGAAATTTCGACATCAGCGTACTATCAAATTACAAATTGAATGCTTTAGAATGCAGTAATGTGAAATGATGTTGCTAATATTGCAGGATGGTTCTGAAATAGATTGTCTTGGTGTTGATACTTTGAATGCTGAAACACTGGAGGTGATATCTCGGTTCATCCCAAAACATATTCAAATTATCAGTAGTTTTGACTAAAGAAATCAATTCACGTGTTCTGCTTTTTAACTTTTTCCCTCTCGTGTACTTCCAGGTAACACGGTTTATTTCAAAGGTAACTTTACTCGCATCAGGTGGAGCTGGACATATGTATCCATCAACAACAAATCCCCCGGTACTTCTCATTTTCCAATTAACAATAATTAATCATGTTATGGTGGGCATATTTATACTAATGTTGGTCCACATCTGGTTAAAAGGTTGCCACAGGAGATGGAATCGCCATGGCTCATCGAGCTCAAGCTGTAGTTTCCAACATGGAGTGAGTAACCGTCTCAGTTATTATGATTTATTGTATGTGGATAATATCTTATTATAATTGCAGAGTTGGTTGATAAGAATACAAAAAGAATTTTACCTTACATTTCTATCATTTCCTTGAGGATGCTGATGTAGCTTTGTTCTTGTAATATTGGTTAGCTTTTAGTTATGTTTCATTTGTTGGGGAATGGTTAAGGGCGATGAAAATCATAATTGTAACTAGATTTTGTTATGTAATGCAGATTTGTGCAGTTCCACCCAACTGCCTTAGCTGATGAAGGCCTTCCGGTCAGACCAAACAAGCCGAGAGAGAACGCTTTTCTTATAACCGAAGCTGTTAGGGGTGATGGAGGTATTCTGTACAATTTAAGTATGGAAAGATTTATGCCATTGTACGATGAGAGAGCTGAGCTCGCTCCTAGAGATGTTGTAGCTAGAAGTATAGATGATCAGCTGAAAAAACGAAATGAGAAGTATGTGCTACTTGATATTAGTCACAAACCCAGGGAAAACGTTCTCTCCCATTTCCCCAACATTGCTGCTGAGTGCCTCAAGTATGGTTTGGACATTACACACCAGCCGATTCCGGTGGTTCCAGCTGCTCATTACATGTGTGGAGGTGTACGAGCTGGGCTTCAGGGTGAGACTAATGTGCGGGGTCTGTACGTTGCAGGCGAGGTTGCGTGCACGGGATTACACGGAGCCAACCGCTTAGCTAGCAACTCGTTGCTCGAAGCTCTAGTTTTTGCACGAAGGGCAGTTGAGCCCTCGGTCGATCATATGAAGGGCTCAAGTCTTGATTCAGGTGCATTGAATAGGTGGGCTAGACCTCTCGTGCCCATGTCCCGAGGAAACGATTTCATGGGCAGAATTATATCAATGACAAAAGATGTGAGGAAACAGTTACAACTAATCATGTGGAAGTATGTCGGAATCGTTCGGTCGACCACCAGGCTCGAGAGGGCGGAGCAGAAGATCGGTGAGCTAGAAGCAACATGGGAAGAGTACTTGTTTCATCAAGGATGGGAGCCAACCATGGTGGCTCTGGAGGTTTGTGAGATGAGGAACCTGTTTTGCTGTGCGAAACTGGTCGTAAGCAGCGCCCTCGCTCGGCAAGAAAGTCGGGGACTTCATTACACGATCGATTTTCCACATCTTGAGGAGAGTAAGAGGCTTCCGACCATCATATTTCCTTGTTCACCCACCAAGAGTACATGGAGCTCAAGGCAGCTCCATTGGCAGCCTGTG

mRNA sequence

ATGGCGATTTGTATACCTGTTGGAAGCTGCAAACTGTTCTGCAGGGCGGTGGAGTTGAAGGGGCAAGGTTTTAGAAGAGCCCAATGGGACGCTAGCATAGCTTTCAAAAGATTCTTTCAGGACGAGCTTTCACGATCATGTAGGTTGAATAAGTTCTCACATCTGCAAAGAAACAATATTTCCCAACCTACAATTAATGAGAAATGGAGAACTATTGCGACAGTCACTTCATCGTGCTTGAGTGACGGTTCAACAAAGTATTTTGATTTTGTTGTTATTGGTAGTGGAGTTGCTGGCCTCCGTTACGCTCTTGAAGTTGCAAAGCATGGATCCGTAGCCGTGATCACTAAGGCTGAGCCTCATGAGAGCAACACCAATTATGCTCAAGGTGGTGTTAGTGCCGTTCTTTGTCCTTCAGACTCTGTTGAAAGTCATGTGCAGGACACCATCGTAGCTGGGGCTCATCTGTGCGACGAGGAGACAGTTAGAGTTGTATGTACAGAAGGGCCTGACAGAATCAAAGAGTTGATTGCTATGGGTGCGTCATTTGATCATGGGGAAGATGGTAACTTACACCTAGCAAGGGAAGGGGGGCACTCTCATCGTAGGATTGTTCATGCTGCTGATATGACTGGAAGGGAAATTGAGCGGGCTCTTTTGGAGGCGGTTGTTCGGGATCCCAACATTTTTGTGTTTGAGCACCATTTTGCAATTGACTTGCTAACTTCCGAGGATGGTTCTGAAATAGATTGTCTTGGTGTTGATACTTTGAATGCTGAAACACTGGAGGTAACACGGTTTATTTCAAAGGTAACTTTACTCGCATCAGGTGGAGCTGGACATATGTATCCATCAACAACAAATCCCCCGGTTGCCACAGGAGATGGAATCGCCATGGCTCATCGAGCTCAAGCTGTAGTTTCCAACATGGAATTTGTGCAGTTCCACCCAACTGCCTTAGCTGATGAAGGCCTTCCGGTCAGACCAAACAAGCCGAGAGAGAACGCTTTTCTTATAACCGAAGCTGTTAGGGGTGATGGAGGTATTCTGTACAATTTAAGTATGGAAAGATTTATGCCATTGTACGATGAGAGAGCTGAGCTCGCTCCTAGAGATGTTGTAGCTAGAAGTATAGATGATCAGCTGAAAAAACGAAATGAGAAGTATGTGCTACTTGATATTAGTCACAAACCCAGGGAAAACGTTCTCTCCCATTTCCCCAACATTGCTGCTGAGTGCCTCAAGTATGGTTTGGACATTACACACCAGCCGATTCCGGTGGTTCCAGCTGCTCATTACATGTGTGGAGGTGTACGAGCTGGGCTTCAGGGTGAGACTAATGTGCGGGGTCTGTACGTTGCAGGCGAGGTTGCGTGCACGGGATTACACGGAGCCAACCGCTTAGCTAGCAACTCGTTGCTCGAAGCTCTAGTTTTTGCACGAAGGGCAGTTGAGCCCTCGGTCGATCATATGAAGGGCTCAAGTCTTGATTCAGGTGCATTGAATAGGTGGGCTAGACCTCTCGTGCCCATGTCCCGAGGAAACGATTTCATGGGCAGAATTATATCAATGACAAAAGATGTGAGGAAACAGTTACAACTAATCATGTGGAAGTATGTCGGAATCGTTCGGTCGACCACCAGGCTCGAGAGGGCGGAGCAGAAGATCGGTGAGCTAGAAGCAACATGGGAAGAGTACTTGTTTCATCAAGGATGGGAGCCAACCATGGTGGCTCTGGAGGTTTGTGAGATGAGGAACCTGTTTTGCTGTGCGAAACTGGTCGTAAGCAGCGCCCTCGCTCGGCAAGAAAGTCGGGGACTTCATTACACGATCGATTTTCCACATCTTGAGGAGAGTAAGAGGCTTCCGACCATCATATTTCCTTGTTCACCCACCAAGAGTACATGGAGCTCAAGGCAGCTCCATTGGCAGCCTGTG

Coding sequence (CDS)

ATGGCGATTTGTATACCTGTTGGAAGCTGCAAACTGTTCTGCAGGGCGGTGGAGTTGAAGGGGCAAGGTTTTAGAAGAGCCCAATGGGACGCTAGCATAGCTTTCAAAAGATTCTTTCAGGACGAGCTTTCACGATCATGTAGGTTGAATAAGTTCTCACATCTGCAAAGAAACAATATTTCCCAACCTACAATTAATGAGAAATGGAGAACTATTGCGACAGTCACTTCATCGTGCTTGAGTGACGGTTCAACAAAGTATTTTGATTTTGTTGTTATTGGTAGTGGAGTTGCTGGCCTCCGTTACGCTCTTGAAGTTGCAAAGCATGGATCCGTAGCCGTGATCACTAAGGCTGAGCCTCATGAGAGCAACACCAATTATGCTCAAGGTGGTGTTAGTGCCGTTCTTTGTCCTTCAGACTCTGTTGAAAGTCATGTGCAGGACACCATCGTAGCTGGGGCTCATCTGTGCGACGAGGAGACAGTTAGAGTTGTATGTACAGAAGGGCCTGACAGAATCAAAGAGTTGATTGCTATGGGTGCGTCATTTGATCATGGGGAAGATGGTAACTTACACCTAGCAAGGGAAGGGGGGCACTCTCATCGTAGGATTGTTCATGCTGCTGATATGACTGGAAGGGAAATTGAGCGGGCTCTTTTGGAGGCGGTTGTTCGGGATCCCAACATTTTTGTGTTTGAGCACCATTTTGCAATTGACTTGCTAACTTCCGAGGATGGTTCTGAAATAGATTGTCTTGGTGTTGATACTTTGAATGCTGAAACACTGGAGGTAACACGGTTTATTTCAAAGGTAACTTTACTCGCATCAGGTGGAGCTGGACATATGTATCCATCAACAACAAATCCCCCGGTTGCCACAGGAGATGGAATCGCCATGGCTCATCGAGCTCAAGCTGTAGTTTCCAACATGGAATTTGTGCAGTTCCACCCAACTGCCTTAGCTGATGAAGGCCTTCCGGTCAGACCAAACAAGCCGAGAGAGAACGCTTTTCTTATAACCGAAGCTGTTAGGGGTGATGGAGGTATTCTGTACAATTTAAGTATGGAAAGATTTATGCCATTGTACGATGAGAGAGCTGAGCTCGCTCCTAGAGATGTTGTAGCTAGAAGTATAGATGATCAGCTGAAAAAACGAAATGAGAAGTATGTGCTACTTGATATTAGTCACAAACCCAGGGAAAACGTTCTCTCCCATTTCCCCAACATTGCTGCTGAGTGCCTCAAGTATGGTTTGGACATTACACACCAGCCGATTCCGGTGGTTCCAGCTGCTCATTACATGTGTGGAGGTGTACGAGCTGGGCTTCAGGGTGAGACTAATGTGCGGGGTCTGTACGTTGCAGGCGAGGTTGCGTGCACGGGATTACACGGAGCCAACCGCTTAGCTAGCAACTCGTTGCTCGAAGCTCTAGTTTTTGCACGAAGGGCAGTTGAGCCCTCGGTCGATCATATGAAGGGCTCAAGTCTTGATTCAGGTGCATTGAATAGGTGGGCTAGACCTCTCGTGCCCATGTCCCGAGGAAACGATTTCATGGGCAGAATTATATCAATGACAAAAGATGTGAGGAAACAGTTACAACTAATCATGTGGAAGTATGTCGGAATCGTTCGGTCGACCACCAGGCTCGAGAGGGCGGAGCAGAAGATCGGTGAGCTAGAAGCAACATGGGAAGAGTACTTGTTTCATCAAGGATGGGAGCCAACCATGGTGGCTCTGGAGGTTTGTGAGATGAGGAACCTGTTTTGCTGTGCGAAACTGGTCGTAAGCAGCGCCCTCGCTCGGCAAGAAAGTCGGGGACTTCATTACACGATCGATTTTCCACATCTTGAGGAGAGTAAGAGGCTTCCGACCATCATATTTCCTTGTTCACCCACCAAGAGTACATGGAGCTCAAGGCAGCTCCATTGGCAGCCTGTG

Protein sequence

MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNISQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEPHESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDITHQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFARRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYVGIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALARQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Homology
BLAST of MS003143 vs. NCBI nr
Match: XP_022133994.1 (L-aspartate oxidase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKR FQDELSRSCRLNKFSHLQRNNI
Sbjct: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRCFQDELSRSCRLNKFSHLQRNNI 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA
Sbjct: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. NCBI nr
Match: XP_008458759.1 (PREDICTED: L-aspartate oxidase, chloroplastic [Cucumis melo])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 604/646 (93.50%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ ++  
Sbjct: 1   MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCGLSKFSHVHKSKA 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
            QP I EKWRT+A VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  YQPIICEKWRTLAAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 241 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMK SSLDSGALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMKSSSLDSGALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. NCBI nr
Match: KAA0037523.1 (L-aspartate oxidase [Cucumis melo var. makuwa] >TYK01998.1 L-aspartate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 604/646 (93.50%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ ++  
Sbjct: 21  MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCGLSKFSHVHKSKA 80

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
            QP I EKWRT+A VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 81  YQPIICEKWRTLAAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 140

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 141 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 200

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 201 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 260

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 261 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 320

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 321 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 380

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 381 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 440

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 441 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 500

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMK SSLDSGALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 501 RRAVEPSVDHMKSSSLDSGALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 560

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 561 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 620

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 621 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 666

BLAST of MS003143 vs. NCBI nr
Match: XP_038890825.1 (L-aspartate oxidase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 603/646 (93.34%), Postives = 621/646 (96.13%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+ KGQG RRAQWDASIAFKR  QDE SRSC L+KFSH+QR+  
Sbjct: 1   MAICIPVGSCKLFCRAVDWKGQGCRRAQWDASIAFKRCLQDEFSRSCGLSKFSHVQRSKT 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQ  INEKWRT+  VTS+CLSDGSTK FDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  SQSIINEKWRTLTAVTSACLSDGSTKSFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRI+ELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIRELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSH RIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHCRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 241 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLP+R +KPRENAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVISNMEFVQFHPTALADEGLPIRLDKPRENAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPREN+LSHFPNIAAECLK+GLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENILSHFPNIAAECLKHGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNV+GLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 TCQPIPVVPAAHYICGGVRAGLQGETNVQGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMK SSLDSGALNRWARP VP S GN FM RIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMKSSSLDSGALNRWARPFVPTSLGNGFMSRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. NCBI nr
Match: XP_004142806.1 (L-aspartate oxidase, chloroplastic [Cucumis sativus] >KAE8648562.1 hypothetical protein Csa_009422 [Cucumis sativus])

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 600/646 (92.88%), Postives = 621/646 (96.13%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ R+  
Sbjct: 1   MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCELSKFSHVYRSKA 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQP I +KWRT+  VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAK GSVAVITKAEP
Sbjct: 61  SQPIICKKWRTLTAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKCGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLC+EETVRVVCTEGPDRIKELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCNEETVRVVCTEGPDRIKELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 241 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHM+ SSLDS ALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMRNSSLDSSALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. ExPASy Swiss-Prot
Match: Q94AY1 (L-aspartate oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AO PE=1 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 2.6e-267
Identity = 475/644 (73.76%), Postives = 534/644 (82.92%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVEL-KGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNN 60
           MA  +  G+   F  A ++ +GQ F    W ++  F      E S S  + K    +R  
Sbjct: 1   MAAHVSTGNIHNFYLAGQVYRGQAF---SWSSASTFMANPFKEPSWSSGVFKALKAERCG 60

Query: 61  ISQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAE 120
                I+    T   + +  +S  STKY+DF VIGSGVAGLRYALEVAK G+VAVITK E
Sbjct: 61  CYSRGISPISETSKPIRAVSVS-SSTKYYDFTVIGSGVAGLRYALEVAKQGTVAVITKDE 120

Query: 121 PHESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAM 180
           PHESNTNYAQGGVSAVLCP DSVESH++DT+VAGAHLCDEETVRVVCTEGP+RI+ELIAM
Sbjct: 121 PHESNTNYAQGGVSAVLCPLDSVESHMRDTMVAGAHLCDEETVRVVCTEGPERIRELIAM 180

Query: 181 GASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAID 240
           GASFDHGEDGNLHLAREGGHSH RIVHAADMTGREIERALLEAV+ DPNI VF+HHFAID
Sbjct: 181 GASFDHGEDGNLHLAREGGHSHCRIVHAADMTGREIERALLEAVLNDPNISVFKHHFAID 240

Query: 241 LLTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM 300
           LLTS+DG    C GVDTLN +T EV RFISKVTLLASGGAGH+YPSTTNP VATGDG+AM
Sbjct: 241 LLTSQDGLNTVCHGVDTLNIKTNEVVRFISKVTLLASGGAGHIYPSTTNPLVATGDGMAM 300

Query: 301 AHRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFM 360
           AHRAQAV+SNMEFVQFHPTALADEGLP++    RENAFLITEAVRGDGGILYNL MERFM
Sbjct: 301 AHRAQAVISNMEFVQFHPTALADEGLPIKLQTARENAFLITEAVRGDGGILYNLGMERFM 360

Query: 361 PLYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLD 420
           P+YDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE +L+HFPNIA+ECLK+GLD
Sbjct: 361 PVYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPREKILAHFPNIASECLKHGLD 420

Query: 421 ITHQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVF 480
           IT QPIPVVPAAHYMCGGVRAGLQGETNV GL+VAGEVACTGLHGANRLASNSLLEALVF
Sbjct: 421 ITRQPIPVVPAAHYMCGGVRAGLQGETNVLGLFVAGEVACTGLHGANRLASNSLLEALVF 480

Query: 481 ARRAVEPSVDHMKGSSLDSGALNRWARPLVPMSR--GNDFMGRIISMTKDVRKQLQLIMW 540
           ARRAV+PS + MK + LD  A  +W RP+V  +R  G++ + +II++TK+VR++LQ +MW
Sbjct: 481 ARRAVQPSTELMKRTRLDVCASEKWTRPVVATARLLGDEVIAKIIALTKEVRRELQEVMW 540

Query: 541 KYVGIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSS 600
           KYVGIVRST RL  AE+KI ELEA WE +LF  GWE T+VALE CEMRNLFCCAKLVVSS
Sbjct: 541 KYVGIVRSTIRLTTAERKIAELEAKWETFLFEHGWEQTVVALEACEMRNLFCCAKLVVSS 600

Query: 601 ALARQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQL 642
           ALAR ESRGLHY  DFP +EESKR+PTII P SPT ++WSSR+L
Sbjct: 601 ALARHESRGLHYMTDFPFVEESKRIPTIILPSSPTTASWSSRRL 640

BLAST of MS003143 vs. ExPASy Swiss-Prot
Match: Q6Z836 (L-aspartate oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0134400 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 6.3e-266
Identity = 452/584 (77.40%), Postives = 505/584 (86.47%), Query Frame = 0

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQ  +  K  +I    +SCL D +TKYFDFVVIGSGVAGLRYALEV+K+GSVA+ITKAEP
Sbjct: 58  SQLRMRHKVGSIRASAASCLQDETTKYFDFVVIGSGVAGLRYALEVSKYGSVAIITKAEP 117

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESH+QDTIVAGA+LCDEETVRVVCTEGP+R+KELIAMG
Sbjct: 118 HESNTNYAQGGVSAVLCPSDSVESHMQDTIVAGAYLCDEETVRVVCTEGPERVKELIAMG 177

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDG LHLAREGGHSH RIVH+ADMTGREIERALL+AV  D NI +F HHFAIDL
Sbjct: 178 ASFDHGEDGRLHLAREGGHSHNRIVHSADMTGREIERALLQAVDNDDNISLFGHHFAIDL 237

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LT +   EI C GVD+L+AET +  RFISKVTLLASGG GH+YPSTTNPPVATGDGIAM+
Sbjct: 238 LTCQSNGEIYCYGVDSLDAETQKAIRFISKVTLLASGGVGHIYPSTTNPPVATGDGIAMS 297

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTAL+DEGLP++P   RENAFLITEAVRGDGGILYN SMERFM 
Sbjct: 298 HRAQAVISNMEFVQFHPTALSDEGLPIKPATRRENAFLITEAVRGDGGILYNQSMERFMT 357

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
            YD+RAELAPRDVVARSIDDQLKKR EKYVLLDISHKPRE VL+HFPNIAAECL++GLDI
Sbjct: 358 SYDDRAELAPRDVVARSIDDQLKKRGEKYVLLDISHKPREKVLAHFPNIAAECLRHGLDI 417

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHYMCGGVRAGLQGETNV+GLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 418 TQQPIPVVPAAHYMCGGVRAGLQGETNVKGLYVAGEVACTGLHGANRLASNSLLEALVFA 477

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAV+PS+DHM  + +D     +WARP++ +S  +  +  II  TK  R +LQ IMW+YV
Sbjct: 478 RRAVQPSIDHMVDADVDPSFAKKWARPVLSVSLRDSILSDIIEKTKQARMELQSIMWEYV 537

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRST RL+ AE KI +LE+ WEE+LF +GW+PTMV +E CEMRNLFCCAKLVV SALA
Sbjct: 538 GIVRSTNRLKHAEWKISDLESEWEEFLFRRGWKPTMVGVETCEMRNLFCCAKLVVKSALA 597

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQ 645
           R ESRGLH+T DFP+LEESKR PT+IFP    + TWSS+ L  Q
Sbjct: 598 RHESRGLHFTEDFPYLEESKRKPTVIFPTHIQELTWSSKPLQKQ 641

BLAST of MS003143 vs. ExPASy Swiss-Prot
Match: Q9KPA4 (L-aspartate oxidase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=nadB PE=3 SV=2)

HSP 1 Score: 436.0 bits (1120), Expect = 7.3e-121
Identity = 252/550 (45.82%), Postives = 342/550 (62.18%), Query Frame = 0

Query: 89  DFVVIGSGVAGLRYALEVAKHGSVAVITKAEPHESNTNYAQGGVSAVLCPSDSVESHVQD 148
           D +VIGSG AGL  AL+VA++G V V++K    E  T YAQGG++AV   SDS+ESHVQD
Sbjct: 10  DVLVIGSGAAGLSLALQVAQYGKVIVLSKGPRSEGATFYAQGGIAAVFDESDSIESHVQD 69

Query: 149 TIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASFDHGEDGN-----LHLAREGGHSHRR 208
           T++AGA +CDE+TVR +     + ++ LI  G  FD  ED +      HL REGGHSHRR
Sbjct: 70  TLIAGAGICDEQTVRFIAEHAKECVQWLIDGGVPFDKEEDSDNDHPRYHLTREGGHSHRR 129

Query: 209 IVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSE----DGSEIDCLGVDTLNA 268
           I+HAAD TG  ++ +L +     PNI V E H A+DL+T +    D +++    V   NA
Sbjct: 130 ILHAADATGMAMQTSLQDNAHNHPNITVLERHNALDLITEDKIGGDANKVVGAYVWNRNA 189

Query: 269 ETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFHPTA 328
           E +E  R  +K  +LA+GGA  +Y  T+NP V++GDGIAMA RA   V+N+EF QFHPT 
Sbjct: 190 EHVETIR--AKFVVLATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFNQFHPTC 249

Query: 329 LADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERAELAPRDVVARSID 388
           L           P    FL+TEA+RG+G  L      RFMP +DERAELAPRD+VAR+ID
Sbjct: 250 L---------YHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID 309

Query: 389 DQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDITHQPIPVVPAAHYMCGGVR 448
            ++K+     + LDISHKP + +  HFP I +  +  G+D+T +PIP+VPAAHY CGGV 
Sbjct: 310 FEMKRLGADCMYLDISHKPADFIEKHFPTIYSRLMDLGIDMTKEPIPIVPAAHYTCGGVM 369

Query: 449 AGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFARRAVEPSVDHMKGSSLDSG 508
              QG+T+++ LY  GEV+ TGLHGANR+ASNSLLE +V+A  A +  +  +  +S+   
Sbjct: 370 VNPQGQTDLKQLYAIGEVSYTGLHGANRMASNSLLECVVYAWSASQDIIAQLPNASMPE- 429

Query: 509 ALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYVGIVRSTTRLERAEQKIGEL 568
           +L  W    V  S           + +    +L+L MW Y+GIVR+  RLERA ++I  L
Sbjct: 430 SLPAWDESQVTCSDEE-------VVLQHNWHELRLFMWDYMGIVRTNKRLERAMRRIQLL 489

Query: 569 EATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALARQESRGLHYTIDFP-HLEE 628
           +    EY     +    V+  + EMRNL   A+L+V  A+ R+ESRGLHYT+D+P  L E
Sbjct: 490 QQETHEY-----YSNFRVSNNLLEMRNLLQVAELMVRCAMQRKESRGLHYTLDYPDQLAE 533

BLAST of MS003143 vs. ExPASy Swiss-Prot
Match: Q51363 (L-aspartate oxidase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=nadB PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.8e-120
Identity = 253/549 (46.08%), Postives = 333/549 (60.66%), Query Frame = 0

Query: 89  DFVVIGSGVAGLRYALEVAKHGSVAVITKAEPHESNTNYAQGGVSAVLCPSDSVESHVQD 148
           D +VIGSG AGL  AL + +H  +AV++K E  + +T +AQGGV+AVL  +D+VESHV+D
Sbjct: 8   DVLVIGSGAAGLSLALTLPEHLRIAVLSKGELSQGSTYWAQGGVAAVLDDTDTVESHVED 67

Query: 149 TIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASFDHGEDGN-------LHLAREGGHSH 208
           T+VAG  LC E+ VR       + I+ LI  G  F   E  N        HL REGGHSH
Sbjct: 68  TLVAGGGLCREDAVRFTVEHSREAIQWLIEQGVPFTRDEQHNHEEGSFEYHLTREGGHSH 127

Query: 209 RRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSEDGS--EIDCLGVDTLNA 268
           RRI+HAAD TG  I   LL    R PNI +     A+DL+T          CLG   LN 
Sbjct: 128 RRIIHAADATGAAIFNTLLAQARRRPNIELLSQRVAVDLITERKLGLPSKRCLGAYVLNR 187

Query: 269 ETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFHPTA 328
           E+ EV  F ++ ++LASGGA  +Y  T+NP   +GDGIAMA RA   V N+EF QFHPT 
Sbjct: 188 ESGEVDTFRARFSVLASGGASKVYLYTSNPDGNSGDGIAMAWRAGCRVGNLEFNQFHPTC 247

Query: 329 LADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERAELAPRDVVARSID 388
           L           P+  +FLITEA+RG+G +L   + ERFMP +D R ELAPRD+VAR+ID
Sbjct: 248 L---------YHPQAKSFLITEALRGEGALLRLPNGERFMPRFDPRGELAPRDIVARAID 307

Query: 389 DQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDITHQPIPVVPAAHYMCGGVR 448
            ++K+     V LDISHKP E + +HFP +   CL +G+DIT QPIPVVPAAHY CGGV 
Sbjct: 308 HEMKRLGIDCVYLDISHKPAEFIKAHFPTVYERCLDFGIDITQQPIPVVPAAHYTCGGVL 367

Query: 449 AGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFARRAVEPSVDHMKGSSLDSG 508
               G T+V GLY  GE   TGLHGANR+ASNSLLE  V+AR A    +  + G+ +   
Sbjct: 368 VDQHGHTDVPGLYAIGETTFTGLHGANRMASNSLLECFVYARSAAADMLQRLPGTPVPE- 427

Query: 509 ALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYVGIVRSTTRLERAEQKIGEL 568
           +L  W    V  S  +     II+   D   +L+  MW YVGIVR+  RL+RA+ ++  L
Sbjct: 428 SLPSWDASQVTDSDED----VIIAHNWD---ELRRFMWDYVGIVRTNKRLQRAQHRVRLL 487

Query: 569 EATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALARQESRGLHYTIDFPHLEES 628
            +  +E+     +    V+ ++ E+RNL   A+L++ SA+ R+ESRGLHYT+D+P L   
Sbjct: 488 LSEIDEF-----YSNYKVSRDLIELRNLALVAELIIRSAMQRRESRGLHYTLDYPDLLPE 533

BLAST of MS003143 vs. ExPASy Swiss-Prot
Match: Q8XWM7 (L-aspartate oxidase 1 OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=nadB1 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 6.1e-120
Identity = 256/545 (46.97%), Postives = 327/545 (60.00%), Query Frame = 0

Query: 88  FDFVVIGSGVAGLRYALEVAKHGSVAVITKAEPHESNTNYAQGGVSAVLCPSDSVESHVQ 147
           FD  V+GSG+AGL  AL +A H  V VI+K    E  +++AQGG++AVL  +DS + HV 
Sbjct: 3   FDVAVVGSGLAGLTVALHLADHRRVVVISKRTLPEGASDWAQGGIAAVLDSNDSHDEHVD 62

Query: 148 DTIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASF--DHGEDGNLHLAREGGHSHRRIV 207
           DT++AGA LCDE   R +   G   I+ LI  G  F  D   +   HL REGGH HRRI+
Sbjct: 63  DTLIAGAGLCDEAATRYIVENGRAAIEWLIGHGVPFTRDARAELGFHLTREGGHRHRRII 122

Query: 208 HAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSEDGS--EIDCLGVDTLNAETLE 267
           HAAD TG  +   L++ V   PNI + E HFAIDL+T        + C G+  L+ +  +
Sbjct: 123 HAADATGHAVVTTLVDKVRAHPNITLLEDHFAIDLVTDAKLGLPGMRCHGLYVLDCKRGD 182

Query: 268 VTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFHPTALADE 327
           V   I+  T+LA+GGAG +Y  TTNP  ATGDGIAMA RA   V+NMEF+QFHPT L   
Sbjct: 183 VKTIIASQTVLATGGAGKVYLYTTNPDTATGDGIAMAWRAGCRVANMEFIQFHPTCL--- 242

Query: 328 GLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERAELAPRDVVARSIDDQLK 387
                   P   +FLI+EAVRG+GG L      RFMP +DERAELAPRD+VAR+ID ++K
Sbjct: 243 ------YHPFAKSFLISEAVRGEGGKLVLPDGTRFMPAHDERAELAPRDIVARAIDFEMK 302

Query: 388 KRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDITHQPIPVVPAAHYMCGGVRAGLQ 447
           KR    V LDISH+    +  HFP I A CL+ G+DIT QPIPVVPAAHY CGGV     
Sbjct: 303 KRGLDCVYLDISHQSPAFIQEHFPTILARCLELGIDITRQPIPVVPAAHYTCGGVVTDQL 362

Query: 448 GETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFARRAVEPSVDHMKGSSLDSGALNR 507
           G T++ GLY  GE A TGLHGANRLASNSLLE +V  R A +  +         + A   
Sbjct: 363 GRTDIAGLYAVGETAYTGLHGANRLASNSLLECMVIGRGAAQDILGQ------PATAPTP 422

Query: 508 WARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYVGIVRSTTRLERAEQKIGELEATW 567
              P    SR  D    ++        +L+ +MW YVGIVR+  RLERA+ +I  L    
Sbjct: 423 TPIPAWDESRVTDADEEVV--VSHNWDELRRMMWNYVGIVRTNKRLERAQHRIALLREEI 482

Query: 568 EEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALARQESRGLHYTIDFPHLEESKRLP 627
            EY     +    V+ ++ E+RNL   A L+V SAL+R ESRGLH++ D+P     K LP
Sbjct: 483 AEY-----YANFRVSHDLLELRNLVEAASLIVDSALSRHESRGLHFSRDYPQ-TLPKALP 524

Query: 628 TIIFP 629
           T++ P
Sbjct: 543 TVMQP 524

BLAST of MS003143 vs. ExPASy TrEMBL
Match: A0A6J1BXI9 (L-aspartate oxidase OS=Momordica charantia OX=3673 GN=LOC111006384 PE=3 SV=1)

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKR FQDELSRSCRLNKFSHLQRNNI
Sbjct: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRCFQDELSRSCRLNKFSHLQRNNI 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA
Sbjct: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. ExPASy TrEMBL
Match: A0A5D3BSM4 (L-aspartate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G001120 PE=3 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 604/646 (93.50%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ ++  
Sbjct: 21  MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCGLSKFSHVHKSKA 80

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
            QP I EKWRT+A VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 81  YQPIICEKWRTLAAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 140

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 141 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 200

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 201 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 260

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 261 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 320

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 321 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 380

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 381 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 440

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 441 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 500

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMK SSLDSGALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 501 RRAVEPSVDHMKSSSLDSGALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 560

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 561 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 620

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 621 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 666

BLAST of MS003143 vs. ExPASy TrEMBL
Match: A0A1S3C8Q4 (L-aspartate oxidase OS=Cucumis melo OX=3656 GN=LOC103498070 PE=3 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 604/646 (93.50%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ ++  
Sbjct: 1   MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCGLSKFSHVHKSKA 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
            QP I EKWRT+A VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  YQPIICEKWRTLAAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 241 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHMK SSLDSGALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVDHMKSSSLDSGALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. ExPASy TrEMBL
Match: A0A0A0KSH5 (L-aspartate oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G524850 PE=3 SV=1)

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 600/646 (92.88%), Postives = 621/646 (96.13%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LKGQG RRAQWDAS+AFKR  QDE SRSC L+KFSH+ R+  
Sbjct: 61  MAICIPVGSCKLFCRAVDLKGQGCRRAQWDASVAFKRCLQDEFSRSCELSKFSHVYRSKA 120

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQP I +KWRT+  VTS+CLSDGSTKYFDFVVIGSGVAGLRYALEVAK GSVAVITKAEP
Sbjct: 121 SQPIICKKWRTLTAVTSACLSDGSTKYFDFVVIGSGVAGLRYALEVAKCGSVAVITKAEP 180

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLC+EETVRVVCTEGPDRIKELIAMG
Sbjct: 181 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCNEETVRVVCTEGPDRIKELIAMG 240

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL
Sbjct: 241 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 300

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 301 LTSEDGSDIVCVGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 360

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVR  KPR+NAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 361 HRAQAVISNMEFVQFHPTALADEGLPVRLEKPRDNAFLITEAVRGDGGILYNLSMERFMP 420

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLK+GLDI
Sbjct: 421 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKHGLDI 480

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 481 TCQPIPVVPAAHYICGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 540

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSVDHM+ SSLDS ALNRWARP VP+S GNDFM RIISMTKDVRKQLQLIMWKYV
Sbjct: 541 RRAVEPSVDHMRNSSLDSSALNRWARPFVPVSLGNDFMSRIISMTKDVRKQLQLIMWKYV 600

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE+KI ELEATWEEYLFHQGWEPTMV LEVCEMRNLFCCAKLVVSSALA
Sbjct: 601 GIVRSTTRLETAERKISELEATWEEYLFHQGWEPTMVGLEVCEMRNLFCCAKLVVSSALA 660

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV
Sbjct: 661 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 706

BLAST of MS003143 vs. ExPASy TrEMBL
Match: A0A6J1HF93 (L-aspartate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111462500 PE=3 SV=1)

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 598/646 (92.57%), Postives = 618/646 (95.67%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVELKGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNNI 60
           MAICIPVGSCKLFCRAV+LK QG R+AQWDASIAFKR  QDE SRS RL+KFSHLQ +  
Sbjct: 1   MAICIPVGSCKLFCRAVDLKWQGCRKAQWDASIAFKRCLQDEFSRSHRLSKFSHLQSSKT 60

Query: 61  SQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAEP 120
           SQPTINEKWRT  TV S+CL+DGSTKYFDFV+IGSGVAGLRYALEVAKHGSVAVITKAEP
Sbjct: 61  SQPTINEKWRTPTTVASACLNDGSTKYFDFVIIGSGVAGLRYALEVAKHGSVAVITKAEP 120

Query: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMG 180
           HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRI+ELIAMG
Sbjct: 121 HESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIRELIAMG 180

Query: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDL 240
           ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVF+HHFAIDL
Sbjct: 181 ASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFQHHFAIDL 240

Query: 241 LTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMA 300
           LTSEDGS+I C+GVDTLN ETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM+
Sbjct: 241 LTSEDGSDIVCVGVDTLNTETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMS 300

Query: 301 HRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMP 360
           HRAQAV+SNMEFVQFHPTALADEGLPVRP+KPRENAFLITEAVRGDGGILYNLSMERFMP
Sbjct: 301 HRAQAVISNMEFVQFHPTALADEGLPVRPDKPRENAFLITEAVRGDGGILYNLSMERFMP 360

Query: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLDI 420
           LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE++LSHFPNIAAECLKYGLDI
Sbjct: 361 LYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRESILSHFPNIAAECLKYGLDI 420

Query: 421 THQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVFA 480
           T QPIPVVPAAHY+CGGVRAGLQGETNV+GLYVAGEVACTGLHGANRLASNSLLEALVFA
Sbjct: 421 TCQPIPVVPAAHYICGGVRAGLQGETNVQGLYVAGEVACTGLHGANRLASNSLLEALVFA 480

Query: 481 RRAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWKYV 540
           RRAVEPSV HMK SS DS ALNRWARP VPMS GN FM RIISMT +VRKQLQLIMWKYV
Sbjct: 481 RRAVEPSVVHMKSSSFDSSALNRWARPFVPMSLGNHFMSRIISMTNNVRKQLQLIMWKYV 540

Query: 541 GIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSSALA 600
           GIVRSTTRLE AE KI ELEATWEEYLFHQGWEPTMVAL+VCEMRNLFCCAKLVVSSALA
Sbjct: 541 GIVRSTTRLETAELKISELEATWEEYLFHQGWEPTMVALQVCEMRNLFCCAKLVVSSALA 600

Query: 601 RQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQLHWQPV 647
           RQESRGLHYTIDFPHLEESKRLPTII PCSPTKSTWSSRQLHWQPV
Sbjct: 601 RQESRGLHYTIDFPHLEESKRLPTIILPCSPTKSTWSSRQLHWQPV 646

BLAST of MS003143 vs. TAIR 10
Match: AT5G14760.1 (L-aspartate oxidase )

HSP 1 Score: 922.5 bits (2383), Expect = 1.8e-268
Identity = 475/644 (73.76%), Postives = 534/644 (82.92%), Query Frame = 0

Query: 1   MAICIPVGSCKLFCRAVEL-KGQGFRRAQWDASIAFKRFFQDELSRSCRLNKFSHLQRNN 60
           MA  +  G+   F  A ++ +GQ F    W ++  F      E S S  + K    +R  
Sbjct: 1   MAAHVSTGNIHNFYLAGQVYRGQAF---SWSSASTFMANPFKEPSWSSGVFKALKAERCG 60

Query: 61  ISQPTINEKWRTIATVTSSCLSDGSTKYFDFVVIGSGVAGLRYALEVAKHGSVAVITKAE 120
                I+    T   + +  +S  STKY+DF VIGSGVAGLRYALEVAK G+VAVITK E
Sbjct: 61  CYSRGISPISETSKPIRAVSVS-SSTKYYDFTVIGSGVAGLRYALEVAKQGTVAVITKDE 120

Query: 121 PHESNTNYAQGGVSAVLCPSDSVESHVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAM 180
           PHESNTNYAQGGVSAVLCP DSVESH++DT+VAGAHLCDEETVRVVCTEGP+RI+ELIAM
Sbjct: 121 PHESNTNYAQGGVSAVLCPLDSVESHMRDTMVAGAHLCDEETVRVVCTEGPERIRELIAM 180

Query: 181 GASFDHGEDGNLHLAREGGHSHRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAID 240
           GASFDHGEDGNLHLAREGGHSH RIVHAADMTGREIERALLEAV+ DPNI VF+HHFAID
Sbjct: 181 GASFDHGEDGNLHLAREGGHSHCRIVHAADMTGREIERALLEAVLNDPNISVFKHHFAID 240

Query: 241 LLTSEDGSEIDCLGVDTLNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAM 300
           LLTS+DG    C GVDTLN +T EV RFISKVTLLASGGAGH+YPSTTNP VATGDG+AM
Sbjct: 241 LLTSQDGLNTVCHGVDTLNIKTNEVVRFISKVTLLASGGAGHIYPSTTNPLVATGDGMAM 300

Query: 301 AHRAQAVVSNMEFVQFHPTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFM 360
           AHRAQAV+SNMEFVQFHPTALADEGLP++    RENAFLITEAVRGDGGILYNL MERFM
Sbjct: 301 AHRAQAVISNMEFVQFHPTALADEGLPIKLQTARENAFLITEAVRGDGGILYNLGMERFM 360

Query: 361 PLYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRENVLSHFPNIAAECLKYGLD 420
           P+YDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPRE +L+HFPNIA+ECLK+GLD
Sbjct: 361 PVYDERAELAPRDVVARSIDDQLKKRNEKYVLLDISHKPREKILAHFPNIASECLKHGLD 420

Query: 421 ITHQPIPVVPAAHYMCGGVRAGLQGETNVRGLYVAGEVACTGLHGANRLASNSLLEALVF 480
           IT QPIPVVPAAHYMCGGVRAGLQGETNV GL+VAGEVACTGLHGANRLASNSLLEALVF
Sbjct: 421 ITRQPIPVVPAAHYMCGGVRAGLQGETNVLGLFVAGEVACTGLHGANRLASNSLLEALVF 480

Query: 481 ARRAVEPSVDHMKGSSLDSGALNRWARPLVPMSR--GNDFMGRIISMTKDVRKQLQLIMW 540
           ARRAV+PS + MK + LD  A  +W RP+V  +R  G++ + +II++TK+VR++LQ +MW
Sbjct: 481 ARRAVQPSTELMKRTRLDVCASEKWTRPVVATARLLGDEVIAKIIALTKEVRRELQEVMW 540

Query: 541 KYVGIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMVALEVCEMRNLFCCAKLVVSS 600
           KYVGIVRST RL  AE+KI ELEA WE +LF  GWE T+VALE CEMRNLFCCAKLVVSS
Sbjct: 541 KYVGIVRSTIRLTTAERKIAELEAKWETFLFEHGWEQTVVALEACEMRNLFCCAKLVVSS 600

Query: 601 ALARQESRGLHYTIDFPHLEESKRLPTIIFPCSPTKSTWSSRQL 642
           ALAR ESRGLHY  DFP +EESKR+PTII P SPT ++WSSR+L
Sbjct: 601 ALARHESRGLHYMTDFPFVEESKRIPTIILPSSPTTASWSSRRL 640

BLAST of MS003143 vs. TAIR 10
Match: AT5G66760.1 (succinate dehydrogenase 1-1 )

HSP 1 Score: 272.3 bits (695), Expect = 9.9e-73
Identity = 199/569 (34.97%), Postives = 282/569 (49.56%), Query Frame = 0

Query: 88  FDFVVIGSGVAGLRYALEVAKHG-SVAVITKAEPHESNTNYAQGGVSAVL--CPSDSVES 147
           +D VV+G+G AGLR A+ +++HG + A ITK  P  S+T  AQGG++A L     D    
Sbjct: 50  YDAVVVGAGGAGLRAAIGLSEHGFNTACITKLFPTRSHTVAAQGGINAALGNMSEDDWRW 109

Query: 148 HVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASFDHGEDGNLHLAREGGHS---- 207
           H+ DT+     L D++ ++ +C E P  + EL   G  F   E+G ++    GG S    
Sbjct: 110 HMYDTVKGSDWLGDQDAIQYMCREAPKAVIELENYGLPFSRTEEGKIYQRAFGGQSLDFG 169

Query: 208 ----HRRIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSEDGSEIDCLGVDT 267
                 R   AAD TG  +   L    ++  N   F  +FA+DLL + DGS   C GV  
Sbjct: 170 KGGQAYRCACAADRTGHALLHTLYGQAMKH-NTQFFVEYFALDLLMASDGS---CQGVIA 229

Query: 268 LNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFH 327
           LN E   + RF S  T+LA+GG G  Y S T+    TGDG AM  RA   + ++EFVQFH
Sbjct: 230 LNMEDGTLHRFRSSQTILATGGYGRAYFSATSAHTCTGDGNAMVARAGLPLQDLEFVQFH 289

Query: 328 PTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERA-ELAPRDVVA 387
           PT +   G             LITE  RG+GGIL N   ERFM  Y   A +LA RDVV+
Sbjct: 290 PTGIYGAGC------------LITEGSRGEGGILRNSEGERFMERYAPTAKDLASRDVVS 349

Query: 388 RSIDDQLKK-----RNEKYVLLDISHKPRENVLSHFPNIAAECLKY-GLDITHQPIPVVP 447
           RS+  ++++      ++ ++ L ++H P E +    P I+     + G+D+T +PIPV+P
Sbjct: 350 RSMTMEIREGRGVGPHKDHIYLHLNHLPPEVLKERLPGISETAAIFAGVDVTKEPIPVLP 409

Query: 448 AAHYMCGGVRAGLQGE----------TNVRGLYVAGEVACTGLHGANRLASNSLLEALVF 507
             HY  GG+     GE            + GL  AGE AC  +HGANRL +NSLL+ +VF
Sbjct: 410 TVHYNMGGIPTNYHGEVVTIKGDDPDAVIPGLMAAGEAACASVHGANRLGANSLLDIVVF 469

Query: 508 AR----RAVEPSVDHMKGSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLI 567
            R    R  E S    K   L+  A  +    L  +   N  +      T  +R  +Q I
Sbjct: 470 GRACANRVAEISKPGEKQKPLEKDAGEKTIAWLDRLRNSNGSL-----PTSTIRLNMQRI 529

Query: 568 MWKYVGIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMV----ALEVCEMRNLFCCA 621
           M     + R+   LE   Q I   +  WE +   Q  + +M+     +E  E+ NL   A
Sbjct: 530 MQNNAAVFRTQETLEEGCQLI---DKAWESFGDVQVKDRSMIWNSDLIETLELENLLINA 589

BLAST of MS003143 vs. TAIR 10
Match: AT2G18450.1 (succinate dehydrogenase 1-2 )

HSP 1 Score: 264.2 bits (674), Expect = 2.7e-70
Identity = 190/565 (33.63%), Postives = 282/565 (49.91%), Query Frame = 0

Query: 88  FDFVVIGSGVAGLRYALEVAKHG-SVAVITKAEPHESNTNYAQGGVSAVL--CPSDSVES 147
           +D VV+G+G AGLR A+ +++HG + A ITK  P  S+T  AQGG++A L     D    
Sbjct: 48  YDAVVVGAGGAGLRAAIGLSEHGFNTACITKLFPTRSHTVAAQGGINAALGNMSVDDWRW 107

Query: 148 HVQDTIVAGAHLCDEETVRVVCTEGPDRIKELIAMGASFDHGEDGNLHLAREGGHSHR-- 207
           H+ DT+     L D++ ++ +C E P  + EL   G  F   EDG ++    GG S    
Sbjct: 108 HMYDTVKGSDWLGDQDAIQYMCREAPKAVIELENYGLPFSRTEDGKIYQRAFGGQSLEFG 167

Query: 208 ------RIVHAADMTGREIERALLEAVVRDPNIFVFEHHFAIDLLTSEDGSEIDCLGVDT 267
                 R   AAD TG  +   L    ++  N   F  +FA+DL+ + DG+   C GV  
Sbjct: 168 IGGQAYRCACAADRTGHALLHTLYGQAMKH-NTQFFVEYFALDLIMNSDGT---CQGVIA 227

Query: 268 LNAETLEVTRFISKVTLLASGGAGHMYPSTTNPPVATGDGIAMAHRAQAVVSNMEFVQFH 327
           LN E   + RF +  T+LA+GG G  Y S T+    TGDG AM  RA   + ++EFVQFH
Sbjct: 228 LNMEDGTLHRFHAGSTILATGGYGRAYFSATSAHTCTGDGNAMVARAGLPLQDLEFVQFH 287

Query: 328 PTALADEGLPVRPNKPRENAFLITEAVRGDGGILYNLSMERFMPLYDERA-ELAPRDVVA 387
           PT +   G             LITE  RG+GGIL N   E+FM  Y   A +LA RDVV+
Sbjct: 288 PTGIYGAGC------------LITEGARGEGGILRNSEGEKFMDRYAPTARDLASRDVVS 347

Query: 388 RSIDDQLKKRN-----EKYVLLDISHKPRENVLSHFPNIAAECLKY-GLDITHQPIPVVP 447
           RS+  ++++       + Y+ L ++H P E +    P I+     + G+D+T +PIPV+P
Sbjct: 348 RSMTMEIRQGRGAGPMKDYLYLYLNHLPPEVLKERLPGISETAAIFAGVDVTREPIPVLP 407

Query: 448 AAHYMCGGVRAGLQGE----------TNVRGLYVAGEVACTGLHGANRLASNSLLEALVF 507
             HY  GG+     GE            V GL  AGE AC  +HGANRL +NSLL+ +VF
Sbjct: 408 TVHYNMGGIPTNYHGEVITLRGDDPDAVVPGLMAAGEAACASVHGANRLGANSLLDIVVF 467

Query: 508 ARRAVEPSVDHMK-GSSLDSGALNRWARPLVPMSRGNDFMGRIISMTKDVRKQLQLIMWK 567
            R       +  K G  L     +   + +  + R  +  G +   T  +R  +Q +M  
Sbjct: 468 GRACANRVAEIQKPGEKLKPLEKDAGEKSIEWLDRIRNSNGSL--PTSKIRLNMQRVMQN 527

Query: 568 YVGIVRSTTRLERAEQKIGELEATWEEYLFHQGWEPTMV----ALEVCEMRNLFCCAKLV 620
              + R+    E  E+    ++ TW+ +   +  + +M+     +E  E+ NL   A + 
Sbjct: 528 NAAVFRTQ---ETLEEGCDLIDKTWDSFGDVKVTDRSMIWNSDLIETMELENLLVNACIT 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133994.10.0e+0099.85L-aspartate oxidase, chloroplastic isoform X1 [Momordica charantia][more]
XP_008458759.10.0e+0093.50PREDICTED: L-aspartate oxidase, chloroplastic [Cucumis melo][more]
KAA0037523.10.0e+0093.50L-aspartate oxidase [Cucumis melo var. makuwa] >TYK01998.1 L-aspartate oxidase [... [more]
XP_038890825.10.0e+0093.34L-aspartate oxidase, chloroplastic [Benincasa hispida][more]
XP_004142806.10.0e+0092.88L-aspartate oxidase, chloroplastic [Cucumis sativus] >KAE8648562.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q94AY12.6e-26773.76L-aspartate oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AO PE=1 SV... [more]
Q6Z8366.3e-26677.40L-aspartate oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q9KPA47.3e-12145.82L-aspartate oxidase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor I... [more]
Q513632.8e-12046.08L-aspartate oxidase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / C... [more]
Q8XWM76.1e-12046.97L-aspartate oxidase 1 OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=na... [more]
Match NameE-valueIdentityDescription
A0A6J1BXI90.0e+0099.85L-aspartate oxidase OS=Momordica charantia OX=3673 GN=LOC111006384 PE=3 SV=1[more]
A0A5D3BSM40.0e+0093.50L-aspartate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G... [more]
A0A1S3C8Q40.0e+0093.50L-aspartate oxidase OS=Cucumis melo OX=3656 GN=LOC103498070 PE=3 SV=1[more]
A0A0A0KSH50.0e+0092.88L-aspartate oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G524850 PE=3 SV=1[more]
A0A6J1HF930.0e+0092.57L-aspartate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111462500 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14760.11.8e-26873.76L-aspartate oxidase [more]
AT5G66760.19.9e-7334.97succinate dehydrogenase 1-1 [more]
AT2G18450.12.7e-7033.63succinate dehydrogenase 1-2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 542..562
NoneNo IPR availablePRINTSPR00368FADPNRcoord: 90..109
score: 36.38
coord: 436..458
score: 28.98
NoneNo IPR availableGENE3D1.20.58.100coord: 521..633
e-value: 2.9E-30
score: 106.1
NoneNo IPR availablePIRSFPIRSF000171SDHA_APRA_LASPOcoord: 82..324
e-value: 1.0E-16
score: 58.2
coord: 417..646
e-value: 1.5E-12
score: 44.4
NoneNo IPR availablePANTHERPTHR42716:SF2L-ASPARTATE OXIDASE, CHLOROPLASTICcoord: 75..642
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 89..484
e-value: 3.8E-157
score: 525.1
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 85..495
IPR027477Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamilyGENE3D3.90.700.10Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domaincoord: 320..442
e-value: 3.8E-157
score: 525.1
IPR027477Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamilySUPERFAMILY56425Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domaincoord: 310..434
IPR015939Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminalPFAMPF02910Succ_DH_flav_Ccoord: 529..619
e-value: 3.9E-16
score: 59.3
IPR005288L-aspartate oxidaseTIGRFAMTIGR00551TIGR00551coord: 88..614
e-value: 2.0E-163
score: 542.6
IPR005288L-aspartate oxidasePANTHERPTHR42716L-ASPARTATE OXIDASEcoord: 75..642
IPR003953FAD-dependent oxidoreductase 2, FAD binding domainPFAMPF00890FAD_binding_2coord: 89..473
e-value: 1.2E-89
score: 301.3
IPR037099Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamilySUPERFAMILY46977Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domaincoord: 527..630

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003143.1MS003143.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009435 NAD biosynthetic process
molecular_function GO:0008734 L-aspartate oxidase activity
molecular_function GO:0016491 oxidoreductase activity