MS003141 (gene) Bitter gourd (TR) v1

Overview


Name	MS003141
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protease Do-like 1, chloroplastic
Location	scaffold234: 689460 .. 692768 (-)
RNA-Seq Expression	MS003141
Synteny	MS003141

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTGCATATTCCCTCGCTTCCACTTTCTTCCATTTCTCTCCACTTTCTCGGCCTCCTAACAGACGACCCACTGCCTTCCTTCTCCCTAAATCCATTTCTCTTCACAATTTCTCCAACCCCACTTGCCATCTTCGATTCCCCATCTTCTCTCTTCTCAAGAAGAACTCCCAAATTTCAGCCACAAATGCCCCAAAACTCACCATTCCCTCGAACCCCTTTGCTTCAATTTGGGAGTCGCTGCTAATTTTCTCCACTTCGGTTCTCTTGTCCTTCTCTCTCTTTGTTACAGATGTGGATCCGGCGGCTGCTTTTGTCGTCACCACGCCCAGGAAATTGCAGACCGATGAGCTCGCCACTGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTGGTTTATATCACCAATCTTGCTGCAAGGTAATTGTACCCTTTTGGAGCTGAGTTCTGATTATAAATTGGGCGTTTCTTACTTTTTCTTTTTCTTTTTTGGGCACGATTGTATGTTTGAAATGTGATTTTGATGTGGGTTTAGGCAGGATGCTTTTACTTTGGATGTGCTGGAGGTACCACAGGGGTCAGGTTCTGGATTTGTGTGGGACAAAGATGGCCATATTGTCACCAATTATCATGTAATTCGTGGTGCTTCTGATCTCAGGTTAGTTCTGAACACTCTAGATTTCTTTGCAGTTTGTTTTCCATATGTCGAAAGTGGCTGATGACTTTATGCTTAACTTCTATGGTTTGAGGTTTCTGATTTTGGTTATGTTTGGAGTATATTTATCAGTTGTTTTAGCATCTTGAGCTACTTGAGTTTGTACTGGTTTGTGTGTGTTTATTACCTCAGGGTCTGTGAAACTTCTAGAAGAACAATACTTAGGTGTCATCTTTTGCCGACCACCATTACAGTTTTGATATGCAGGAGAGAGAAATATAGAAAAAGAGATATATATTTTCTTTTTTCTGATTTTCAAAAGAATGTAAGGGATGTCAAATTTCTAGTGATGGTCAGTAGCGAGATGAGTGTTGACATGTTATTATCCAAGTATTTTTCCTTCTAAAGTAAATCACTCACGAACATCATTGAAGCATTTAATTGTAGAACCTCCATGTTGCTAATGGTCCTCAAAGAACGCTTTTAGGCTTTATATTTTTCGGAAATTTACGACCACATTTGGCTATGTTAAGAAAAAGAGATTTCTGAGGCTTGCATTGTGGGAAATTTCCTTATGGAGTTTAATGAGTAAATTAGATTCTCTCAAGTTTGATATATTAGAGATTTGCTCCTTTGCCTATAAAGGGTGGTCTTTCTTTATGAAAAAATGTGGTATTGCTGGATGCCTTCCCCGGTCAATAGAGGATTGGCTTCTAGAGGTGGCTCGGAGGATCCTTGAAAGAAAAGGCAAAATTTTTGTGGAGTTGTGTGGTTAGAGCTTTAATTTGGGGCCTTCTAGAGGAACCAAAGAATCTTCAACAACTCATCGTTATCCTTTGATATTTTTTGTGATAATGTACCAATAACTGCAACCTCATAGAAAATTCTTTTGTAATTACATTATAGTCTGTTCCAACAAGATTGGCAAGCTGTAATGTAGTAGCTCTTTGGGAGGGCCTCCTCGGCCCTTGCCTCTAGAATGTTGTTCCCCTTCTTTTTGTTCGTTATAATATATCTTCGTTTCTTATATTAAAAAAAAGGATATTTTAGAGATATTTCTCTAGTCAACACTCAATAGCTTTTTTCTAACTCTTTCTTGCCTTGGGAGAATGATTTCAATGAGGTCCTTTTTTATCCAAAAAATATCATCAAGTTCTTTTATTCCTTGTATTGAGCTAAAATTTTGGATTTGATTTTGGACTCTGGACTTAGAGGTGGCTCCTTATTATTCACATTTAAACTGGTTTTAGTTATGTACAGAGTCACTCTAGCCGACCAAACTACATACGATGCTAAAGTTGTCGGGTTCGACCAAGACAAGGATGTTGCTGTCTTACGTATCGAGGCGCCCAAAGACAAGCTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTGTTTGCTATTGGAAACCCTGTAAGTTTTCCTATTCACTGCTTTGGCCTTCATGTTCAGGTTATTCATTTCTTAAAACAAAAAATGAACCATCAATCTATGATGGAGCATTGTTTGGAAAACAGAATTCACCATTAAAACCATTCTCTCAGAATATTACTATATAATACAATGTTTCTTTGTTCTCTTGTACGTTTCCCATTTGATGTCCTAAAGTATGATCATTATGTTTGTGCAGTTTGGACTTGATCATACTCTTACGACTGGCGTAATCAGGTACGAAGCCTATTTAGAAAATTTTCTTTTAATTTGGTTATACAAATCTTATACTATAATTGAGCCCCATAAACATTTTAGAGCATCATAGTCATGGACGATAATATATTTATCTTTTTGTGGTCGTCGAAATGAGAAGTGAATTCGCGATTCAGCGTATATGAGATATGTTAATTTTCTACTTTTACTGTTTGATTGCTGTTAGAACCTGTATTTCCAAGCTTGGAGAACGGAGAGTGTTGTTCTAAGCATTCATCTTGCTCTTGACAGTGGCCTTCGCCGAGAAATTAGTTCTGCTGCAACTGGTAGGCCCATACAAGATGTCATACAGACAGATGCTGCAATTAATCCGGGCAACAGCGGAGGGCCGCTTCTTGATAGTTCGGGAAACCTTATCGGAATAAACACCGCAATATATTCTCCATCAGGGGCATCCTCAGGCGTTGGGTTTTCAATCCCAGTTGACACAGTTAGTAAACAATGACTTATATTTAACAAGCTGCTGAAGTTCCCTTGGTTTGAAGTCGGTTATTCATCAATATTCTGTCAATGTAGGTGAGTGGCATTGTTGAACAGTTGGTGCAGTTTGGGAAGGTGACGAGACCGATTTTAGGAATTAAGTTTGCTCCTGATCAGTCTGTCGAACAGTTGGGCGTGAGCGGAGTGCTCGTCTTAGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGTATTCATTTCCTCTCTTTTTTCTCTTTTCATTTCTATTCTTGTACCTATAGGCTATAGTTTTCAATATTCCATTATGGTTCCAAGAACTCCATCTGATCACTTCACGTTATGCAGGGCTTGCTACCAACTAAGCGGGATGCTTACGGGAGACTTATATTGGGAGACATAATAACATCAGTGAACGGGAAGAAGGTGACGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTACTC

mRNA sequence

ATGGCTGCTGCATATTCCCTCGCTTCCACTTTCTTCCATTTCTCTCCACTTTCTCGGCCTCCTAACAGACGACCCACTGCCTTCCTTCTCCCTAAATCCATTTCTCTTCACAATTTCTCCAACCCCACTTGCCATCTTCGATTCCCCATCTTCTCTCTTCTCAAGAAGAACTCCCAAATTTCAGCCACAAATGCCCCAAAACTCACCATTCCCTCGAACCCCTTTGCTTCAATTTGGGAGTCGCTGCTAATTTTCTCCACTTCGGTTCTCTTGTCCTTCTCTCTCTTTGTTACAGATGTGGATCCGGCGGCTGCTTTTGTCGTCACCACGCCCAGGAAATTGCAGACCGATGAGCTCGCCACTGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTGGTTTATATCACCAATCTTGCTGCAAGGCAGGATGCTTTTACTTTGGATGTGCTGGAGGTACCACAGGGGTCAGGTTCTGGATTTGTGTGGGACAAAGATGGCCATATTGTCACCAATTATCATGTAATTCGTGGTGCTTCTGATCTCAGAGTCACTCTAGCCGACCAAACTACATACGATGCTAAAGTTGTCGGGTTCGACCAAGACAAGGATGTTGCTGTCTTACGTATCGAGGCGCCCAAAGACAAGCTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTGTTTGCTATTGGAAACCCTTTTGGACTTGATCATACTCTTACGACTGGCGTAATCAGTGGCCTTCGCCGAGAAATTAGTTCTGCTGCAACTGGTAGGCCCATACAAGATGTCATACAGACAGATGCTGCAATTAATCCGGGCAACAGCGGAGGGCCGCTTCTTGATAGTTCGGGAAACCTTATCGGAATAAACACCGCAATATATTCTCCATCAGGGGCATCCTCAGGCGTTGGGTTTTCAATCCCAGTTGACACAGTGAGTGGCATTGTTGAACAGTTGGTGCAGTTTGGGAAGGTGACGAGACCGATTTTAGGAATTAAGTTTGCTCCTGATCAGTCTGTCGAACAGTTGGGCGTGAGCGGAGTGCTCGTCTTAGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGGCTTGCTACCAACTAAGCGGGATGCTTACGGGAGACTTATATTGGGAGACATAATAACATCAGTGAACGGGAAGAAGGTGACGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTACTC

Coding sequence (CDS)

ATGGCTGCTGCATATTCCCTCGCTTCCACTTTCTTCCATTTCTCTCCACTTTCTCGGCCTCCTAACAGACGACCCACTGCCTTCCTTCTCCCTAAATCCATTTCTCTTCACAATTTCTCCAACCCCACTTGCCATCTTCGATTCCCCATCTTCTCTCTTCTCAAGAAGAACTCCCAAATTTCAGCCACAAATGCCCCAAAACTCACCATTCCCTCGAACCCCTTTGCTTCAATTTGGGAGTCGCTGCTAATTTTCTCCACTTCGGTTCTCTTGTCCTTCTCTCTCTTTGTTACAGATGTGGATCCGGCGGCTGCTTTTGTCGTCACCACGCCCAGGAAATTGCAGACCGATGAGCTCGCCACTGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTGGTTTATATCACCAATCTTGCTGCAAGGCAGGATGCTTTTACTTTGGATGTGCTGGAGGTACCACAGGGGTCAGGTTCTGGATTTGTGTGGGACAAAGATGGCCATATTGTCACCAATTATCATGTAATTCGTGGTGCTTCTGATCTCAGAGTCACTCTAGCCGACCAAACTACATACGATGCTAAAGTTGTCGGGTTCGACCAAGACAAGGATGTTGCTGTCTTACGTATCGAGGCGCCCAAAGACAAGCTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTGTTTGCTATTGGAAACCCTTTTGGACTTGATCATACTCTTACGACTGGCGTAATCAGTGGCCTTCGCCGAGAAATTAGTTCTGCTGCAACTGGTAGGCCCATACAAGATGTCATACAGACAGATGCTGCAATTAATCCGGGCAACAGCGGAGGGCCGCTTCTTGATAGTTCGGGAAACCTTATCGGAATAAACACCGCAATATATTCTCCATCAGGGGCATCCTCAGGCGTTGGGTTTTCAATCCCAGTTGACACAGTGAGTGGCATTGTTGAACAGTTGGTGCAGTTTGGGAAGGTGACGAGACCGATTTTAGGAATTAAGTTTGCTCCTGATCAGTCTGTCGAACAGTTGGGCGTGAGCGGAGTGCTCGTCTTAGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGGCTTGCTACCAACTAAGCGGGATGCTTACGGGAGACTTATATTGGGAGACATAATAACATCAGTGAACGGGAAGAAGGTGACGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTACTC

Protein sequence

MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL

Homology

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BLAST of MS003141 vs. NCBI nr
Match: XP_022134109.1 (protease Do-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 805.8 bits (2080), Expect = 1.7e-229
Identity = 414/415 (99.76%), Postives = 414/415 (99.76%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI 60
           MAAAYSLASTFFHFSPLSRPPNRR TAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI
Sbjct: 1   MAAAYSLASTFFHFSPLSRPPNRRSTAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI 60

Query: 61  SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA 120
           SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA
Sbjct: 61  SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 415

[Close]

BLAST of MS003141 vs. NCBI nr
Match: XP_022990583.1 (protease Do-like 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 754.2 bits (1946), Expect = 5.9e-214
Identity = 389/419 (92.84%), Postives = 403/419 (96.18%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+CH R+PIFSLL   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KLT PSNPFASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVG+KV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVI 419

[Close]

BLAST of MS003141 vs. NCBI nr
Match: XP_023515810.1 (protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753.1 bits (1943), Expect = 1.3e-213
Identity = 388/419 (92.60%), Postives = 402/419 (95.94%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+CH R+PIFS+L   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSILGHNKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KLT PSNPFASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVI 419

[Close]

BLAST of MS003141 vs. NCBI nr
Match: XP_022922573.1 (protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 747.3 bits (1928), Expect = 7.2e-212
Identity = 387/419 (92.36%), Postives = 400/419 (95.47%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+ H R+PIFSLL   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KLT PSNP ASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVI 419

[Close]

BLAST of MS003141 vs. NCBI nr
Match: KAG7033121.1 (Protease Do-like 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 745.3 bits (1923), Expect = 2.7e-211
Identity = 386/419 (92.12%), Postives = 399/419 (95.23%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+ H R+PIFSLL   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KL  PSNP ASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVI 419

[Close]

BLAST of MS003141 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 589.3 bits (1518), Expect = 3.3e-167
Identity = 322/412 (78.16%), Postives = 347/412 (84.22%), Query Frame = 0


Query: 7   LASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLR-FPIFSLLKKNSQISATNA 66
           L+ST F    L  PP+   + F L  S S      P    R F I S L  N   +  + 
Sbjct: 11  LSSTLF----LHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLND--NNRDD 70

Query: 67  PKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTD--VDPAAAFVVTTPRKLQTDELATVR 126
              T+   PF+++ +   +  TSV LSFSLF     V+ A+AFVV+TP+KLQTDELATVR
Sbjct: 71  DDDTLHFTPFSAV-KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVR 130

Query: 127 LFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRV 186
           LFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDK GHIVTNYHVIRGASDLRV
Sbjct: 131 LFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGASDLRV 190

Query: 187 TLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLD 246
           TLADQTT+DAKVVGFDQDKDVAVLRI+APK+KLRPIPVG+SADLLVGQKVFAIGNPFGLD
Sbjct: 191 TLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLD 250

Query: 247 HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSP 306
           HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG LIGINTAIYSP
Sbjct: 251 HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSP 310

Query: 307 SGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPAN 366
           SGASSGVGFSIPVDTV GIV+QLV+FGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAP +
Sbjct: 311 SGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPPS 370

Query: 367 GPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD+V
Sbjct: 371 GPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEV 415

[Close]

BLAST of MS003141 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.1e-77
Identity = 164/308 (53.25%), Postives = 204/308 (66.23%), Query Frame = 0


Query: 122 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDL 181
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G+IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 182 RVTLAD-------------QTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLL 241
             +  D             Q  ++ K+VG D+ KD+AVL+++AP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 242 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 301
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 302 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVE 361
           S GNLIGINTAI++ +G S+GVGF+IP  TV  IV QL+QF KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 362 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILD 416
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 419

[Close]

BLAST of MS003141 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 197.6 bits (501), Expect = 2.8e-49
Identity = 125/277 (45.13%), Postives = 169/277 (61.01%), Query Frame = 0


Query: 82  LLIFSTSVLLSFSLFVTD-----VDPAAAF--VVTTPRKLQTDELATVRLFQENTPSVVY 141
           ++IF +S+ L+ SL  ++     ++ A A         +L+ +E   V LFQ+ +PSVVY
Sbjct: 45  IMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSVVY 104

Query: 142 ITNL----AARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIR-------GASDLRV 201
           I  +     +  D  T +     +G+GSGFVWDK GHIVTNYHVI        G    +V
Sbjct: 105 IEAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFGLQRCKV 164

Query: 202 TLAD----QTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNP 261
           +L D    + + + K+VG D D D+AVL+IE    +L P+ +G S DL VGQ  FAIGNP
Sbjct: 165 SLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNP 224

Query: 262 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 321
           +G ++TLT GV+SGL REI S   G+ I + IQTDA IN GNSGGPLLDS G+ IG+NTA
Sbjct: 225 YGYENTLTIGVVSGLGREIPS-PNGKSISEAIQTDADINSGNSGGPLLDSYGHTIGVNTA 284

Query: 322 IYS--PSGASSGVGFSIPVDTVSGIVEQLVQFGKVTR 335
            ++   SG SSGV F+IP+DTV   V  L+ +G   R
Sbjct: 285 TFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

[Close]

BLAST of MS003141 vs. ExPASy Swiss-Prot
Match: Q2YMX6 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2308) OX=359391 GN=htrA PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.1e-49
Identity = 114/269 (42.38%), Postives = 154/269 (57.25%), Query Frame = 0


Query: 151 EVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIE 210
           E P   GSGFV  +DG++VTN HV+       V L D T  DAK++G D   D+AVL+I 
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 211 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 270
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 271 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFG 330
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +V+QL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 331 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 390
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 391 DIITSVNGKKVTNGSDLYRILDQCKVGDK 415
           D+IT+VNG+ V +  DL R +     G+K
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEK 383

[Close]

BLAST of MS003141 vs. ExPASy Swiss-Prot
Match: P0C114 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 (strain 9-941) OX=262698 GN=htrA PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.1e-49
Identity = 114/269 (42.38%), Postives = 154/269 (57.25%), Query Frame = 0


Query: 151 EVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIE 210
           E P   GSGFV  +DG++VTN HV+       V L D T  DAK++G D   D+AVL+I 
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 211 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 270
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 271 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFG 330
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +V+QL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 331 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 390
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 391 DIITSVNGKKVTNGSDLYRILDQCKVGDK 415
           D+IT+VNG+ V +  DL R +     G+K
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEK 383

[Close]

BLAST of MS003141 vs. ExPASy TrEMBL
Match: A0A6J1BXV6 (protease Do-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006463 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 8.3e-230
Identity = 414/415 (99.76%), Postives = 414/415 (99.76%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI 60
           MAAAYSLASTFFHFSPLSRPPNRR TAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI
Sbjct: 1   MAAAYSLASTFFHFSPLSRPPNRRSTAFLLPKSISLHNFSNPTCHLRFPIFSLLKKNSQI 60

Query: 61  SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA 120
           SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA
Sbjct: 61  SATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTDELA 120

Query: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180
           TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD
Sbjct: 121 TVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASD 180

Query: 181 LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240
           LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF
Sbjct: 181 LRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPF 240

Query: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300
           GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI
Sbjct: 241 GLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAI 300

Query: 301 YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360
           YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA
Sbjct: 301 YSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDA 360

Query: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV
Sbjct: 361 PANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 415

[Close]

BLAST of MS003141 vs. ExPASy TrEMBL
Match: A0A6J1JTP9 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.9e-214
Identity = 389/419 (92.84%), Postives = 403/419 (96.18%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+CH R+PIFSLL   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KLT PSNPFASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVG+KV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVI 419

[Close]

BLAST of MS003141 vs. ExPASy TrEMBL
Match: A0A6J1E723 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.5e-212
Identity = 387/419 (92.36%), Postives = 400/419 (95.47%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTFFHFSPLSRPPN RPT+F L KSI  HNFSNP+ H R+PIFSLL   KK 
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KLT PSNP ASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVL 417
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV+
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVI 419

[Close]

BLAST of MS003141 vs. ExPASy TrEMBL
Match: A0A6J1K2S4 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489550 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.1e-209
Identity = 381/418 (91.15%), Postives = 397/418 (94.98%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTF HFSP SRPPNR PT+F LPKSISL NF++P+ HLR+PI SLL   K +
Sbjct: 1   MAAAFSLASTFSHFSPPSRPPNRTPTSFFLPKSISLQNFTDPSSHLRYPICSLLPHNKNH 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KL  PSNPFASIWESLLIFSTS LLSFSLF+TDV+PA AFVVTTPRKLQTD
Sbjct: 61  TQISATTHTKLPTPSNPFASIWESLLIFSTSALLSFSLFLTDVEPAVAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGH+VTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVL I+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV+FGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           LDAPANGPAGKAGL PTKRDAYGRLILGDIITSV+GKKV NGSDLYRILDQCKVGDKV
Sbjct: 361 LDAPANGPAGKAGLRPTKRDAYGRLILGDIITSVDGKKVMNGSDLYRILDQCKVGDKV 418

[Close]

BLAST of MS003141 vs. ExPASy TrEMBL
Match: A0A6J1FEU2 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445066 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.8e-208
Identity = 381/418 (91.15%), Postives = 394/418 (94.26%), Query Frame = 0


Query: 1   MAAAYSLASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60
           MAAA+SLASTF HFSP SRPPNR PT F L KSISL NF+NP+ HLR+PI SLL   K +
Sbjct: 1   MAAAFSLASTFSHFSPPSRPPNRTPTPFFLSKSISLQNFTNPSSHLRYPICSLLPHNKNH 60

Query: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KL  PSNPFAS WESLLIFSTS LLSFSLF+TDVDPA AFVVTTPRKLQTD
Sbjct: 61  TQISATTHTKLPPPSNPFASTWESLLIFSTSALLSFSLFLTDVDPAVAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGH+VTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVL I+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV+FGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           LDAPANGPAGKAGL PTKRDAYGRLILGDIITSV+GKKV NGSDLYRILDQCKVGDKV
Sbjct: 361 LDAPANGPAGKAGLRPTKRDAYGRLILGDIITSVDGKKVMNGSDLYRILDQCKVGDKV 418

[Close]

BLAST of MS003141 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 589.3 bits (1518), Expect = 2.3e-168
Identity = 322/412 (78.16%), Postives = 347/412 (84.22%), Query Frame = 0


Query: 7   LASTFFHFSPLSRPPNRRPTAFLLPKSISLHNFSNPTCHLR-FPIFSLLKKNSQISATNA 66
           L+ST F    L  PP+   + F L  S S      P    R F I S L  N   +  + 
Sbjct: 11  LSSTLF----LHSPPSSHLSFFNLSSSRSSPISLYPIRSKRYFRILSKLSLND--NNRDD 70

Query: 67  PKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTD--VDPAAAFVVTTPRKLQTDELATVR 126
              T+   PF+++ +   +  TSV LSFSLF     V+ A+AFVV+TP+KLQTDELATVR
Sbjct: 71  DDDTLHFTPFSAV-KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVR 130

Query: 127 LFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDLRV 186
           LFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDK GHIVTNYHVIRGASDLRV
Sbjct: 131 LFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGASDLRV 190

Query: 187 TLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLD 246
           TLADQTT+DAKVVGFDQDKDVAVLRI+APK+KLRPIPVG+SADLLVGQKVFAIGNPFGLD
Sbjct: 191 TLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLD 250

Query: 247 HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSP 306
           HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG LIGINTAIYSP
Sbjct: 251 HTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSP 310

Query: 307 SGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPAN 366
           SGASSGVGFSIPVDTV GIV+QLV+FGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAP +
Sbjct: 311 SGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPPS 370

Query: 367 GPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKV 416
           GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD+V
Sbjct: 371 GPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEV 415

[Close]

BLAST of MS003141 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 292.0 bits (746), Expect = 7.7e-79
Identity = 164/308 (53.25%), Postives = 204/308 (66.23%), Query Frame = 0


Query: 122 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDL 181
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G+IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 182 RVTLAD-------------QTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLL 241
             +  D             Q  ++ K+VG D+ KD+AVL+++AP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 242 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 301
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 302 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVE 361
           S GNLIGINTAI++ +G S+GVGF+IP  TV  IV QL+QF KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 362 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILD 416
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 419

[Close]

BLAST of MS003141 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 267.3 bits (682), Expect = 2.0e-71
Identity = 156/308 (50.65%), Postives = 193/308 (62.66%), Query Frame = 0


Query: 122 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRGASDL 181
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G+IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 182 RVTLAD-------------QTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLL 241
             +  D             Q  ++ K+VG D+ KD+AVL+++AP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 242 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 301
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 302 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVE 361
           S GNLIGINTAI++               TV  IV QL+QF KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFT--------------QTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 362 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILD 416
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 412

[Close]

BLAST of MS003141 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-50
Identity = 125/277 (45.13%), Postives = 169/277 (61.01%), Query Frame = 0


Query: 82  LLIFSTSVLLSFSLFVTD-----VDPAAAF--VVTTPRKLQTDELATVRLFQENTPSVVY 141
           ++IF +S+ L+ SL  ++     ++ A A         +L+ +E   V LFQ+ +PSVVY
Sbjct: 45  IMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSVVY 104

Query: 142 ITNL----AARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIR-------GASDLRV 201
           I  +     +  D  T +     +G+GSGFVWDK GHIVTNYHVI        G    +V
Sbjct: 105 IEAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFGLQRCKV 164

Query: 202 TLAD----QTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIGNP 261
           +L D    + + + K+VG D D D+AVL+IE    +L P+ +G S DL VGQ  FAIGNP
Sbjct: 165 SLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNP 224

Query: 262 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 321
           +G ++TLT GV+SGL REI S   G+ I + IQTDA IN GNSGGPLLDS G+ IG+NTA
Sbjct: 225 YGYENTLTIGVVSGLGREIPS-PNGKSISEAIQTDADINSGNSGGPLLDSYGHTIGVNTA 284

Query: 322 IYS--PSGASSGVGFSIPVDTVSGIVEQLVQFGKVTR 335
            ++   SG SSGV F+IP+DTV   V  L+ +G   R
Sbjct: 285 TFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

[Close]

BLAST of MS003141 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-32
Identity = 100/290 (34.48%), Postives = 147/290 (50.69%), Query Frame = 0


Query: 135 ITNLAARQDAFTLDVLEVPQG---------SGSGFVWDKDGHIVTNYHVIRGASDLR--- 194
           I N AAR     ++ L VPQG          GSG + D DG I+T  HV+    ++R   
Sbjct: 119 IANAAARIGPAVVN-LSVPQGFHGISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSS 178

Query: 195 -----VTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 254
                VTL D  T++  VV  D   D+A+++I++ K  L    +G S+ L  G  V A+G
Sbjct: 179 KGRVDVTLQDGRTFEGVVVNADLQSDIALVKIKS-KTPLPTAKLGFSSKLRPGDWVIAVG 238

Query: 255 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 314
            P  L +T+T G++S + R+ S    G   ++ +QTD +IN GNSGGPL++  G +IG+N
Sbjct: 239 CPLSLQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVN 298

Query: 315 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAP--DQSVEQLG---- 374
                   A+ G+GFS+P+D+VS I+E   + G+V RP +G+K     +  V QL     
Sbjct: 299 IMKVL---AADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDP 358

Query: 375 -----VSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV 397
                  GVLV       PA +AG  P           GD++   +GK V
Sbjct: 359 MFPDVERGVLVPTVIPGSPADRAGFKP-----------GDVVVRFDGKPV 392

The following BLAST results are available for this feature:

BLAST of MS003141 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134109.1	1.7e-229	99.76	protease Do-like 1, chloroplastic [Momordica charantia]	[more]
XP_022990583.1	5.9e-214	92.84	protease Do-like 1, chloroplastic [Cucurbita maxima]	[more]
XP_023515810.1	1.3e-213	92.60	protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022922573.1	7.2e-212	92.36	protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do...	[more]
KAG7033121.1	2.7e-211	92.12	Protease Do-like 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS003141 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22609	3.3e-167	78.16	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q9LU10	1.1e-77	53.25	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
Q9SEL7	2.8e-49	45.13	Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ...	[more]
Q2YMX6	8.1e-49	42.38	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2...	[more]
P0C114	8.1e-49	42.38	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 ...	[more]

BLAST of MS003141 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXV6	8.3e-230	99.76	protease Do-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006463...	[more]
A0A6J1JTP9	2.9e-214	92.84	protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE...	[more]
A0A6J1E723	3.5e-212	92.36	protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 ...	[more]
A0A6J1K2S4	2.1e-209	91.15	protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489550 PE...	[more]
A0A6J1FEU2	1.8e-208	91.15	protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445066 ...	[more]

BLAST of MS003141 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27925.1	2.3e-168	78.16	DegP protease 1	[more]
AT5G39830.1	7.7e-79	53.25	Trypsin family protein with PDZ domain	[more]
AT5G39830.2	2.0e-71	50.65	Trypsin family protein with PDZ domain	[more]
AT4G18370.1	2.0e-50	45.13	DEGP protease 5	[more]
AT5G27660.1	4.2e-32	34.48	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 229..253 score: 55.41 coord: 267..284 score: 72.49 coord: 381..393 score: 44.84 coord: 166..178 score: 59.1 coord: 289..306 score: 52.85 coord: 187..207 score: 43.44
IPR001478	PDZ domain	PFAM	PF13180	PDZ_2	coord: 335..415 e-value: 6.0E-9 score: 36.3
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 326..398 score: 10.7775
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 220..334 e-value: 1.6E-41 score: 143.1
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 103..219 e-value: 9.7E-40 score: 136.8
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 335..416 e-value: 1.7E-30 score: 106.8
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 300..415
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 157..296 e-value: 3.1E-36 score: 125.6
None	No IPR available	PANTHER	PTHR43019:SF38	PROTEASE DO-LIKE 1, CHLOROPLASTIC	coord: 52..415
None	No IPR available	PANTHER	PTHR43019	SERINE ENDOPROTEASE DEGS	coord: 52..415
None	No IPR available	CDD	cd00987	PDZ_serine_protease	coord: 335..416 e-value: 2.72184E-14 score: 66.124
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 122..332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS003141.1	MS003141.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004252	serine-type endopeptidase activity