MS003076 (gene) Bitter gourd (TR) v1

Overview
NameMS003076
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDolichyl-phosphate beta-glucosyltransferase
Locationscaffold234: 264217 .. 270490 (-)
RNA-Seq ExpressionMS003076
SyntenyMS003076
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTATCCATGGACGATATTCTTCATTTTGATCAGCCTTTTTTTTACTTTCGCAGCAGGTTTGGTTTCTGCTATCATCCTCAAAGCTTATAGGAGGAGATTCAATCACGAGTACGCTACTTCTGCTTCCTCATTGATATTATATGATATGATATGCAATTTTTAATTTTCTAATCGTCGTATATCTGTCTGGATGAGATATTCATTTGGTTCGTGTTAGGCACGTTGAAGCTCCTGCAATCTTGGAGGACCCAAACTCGTTGAAACGGGTAACTTTTGATCCATTTTCTTTGAGCTTCGATTGGGACTTTTATATGTCAGAGATCACGTTTGATCGCTTGAAAGATTGAATAATACTTTTGTTAATATGGACTCTCGAGCTTGTTAGTATTTCAAATTTTCAATCACTCCAAGCTTGTCTTTGAAGAAATAAAATTTTTAGGCAGCTAGAGGTTTGGTTTAGTCAAACGATCTGCAATTTGTTCGGAAGATTGTATTTACCTTAGCTCTAGTTCTGTGTTGATAACTTTATCTCCTATGGAATGGAGATCTAACCCTAGATGTTTGGTCCGTACATGGTAGACTGGGTTTTACAGCCAAGGCACAAACACTATTGTTATGACACCAAGAGATAGGACACGAGGACAGATCAATGTGAAGTTCTTTTGAGAATAGACTGGTCCAAGTTAACTCTACAATCACATTAGCTTAAGATCTATAGTCTTTGTTCTGCATTTAACCTGGACTCAACACCTTGCTTCTTGGATTTTCTTGGAACTAAACTTTCACCAAGATAGACACAGTGTTCTCCTATGGATCTTCTACCCTCCTTGCTTCATGCCCCATTGGTATCTAAGAAACTAAGCAAACGGATTGTCTCACAAGGTTTTATATGGAGGCTTGATGTTCCCCTCAAGTATGGAAGGATTCTTTTGACCCCTTACGAATGACGGGTGTTGGACTTTGGAATTGTTGACCTAATTTGTTCACACTGCAAGTTATGTTGGGTGTTGCGTAAGCTAAATATTGTTGAGCCCCTATTGCACTTCTGAAAGTTCTTGGGTCACTCATCAATTCATATTCCACATTTTTTAATTCCTTAGTACAGCAGGAGTGGAGCAGGCCTTCAAGTGTAGTTCAATTTTCTCAAGATAACAATGACATACTTGCATTGTTATAGATAGATCCCAGAGTCATCTTTTCATATGGTCCCAAAAGGTAATGCAACTCACCAAGATTCTTAGATACGATGTGTTTGCTTTGTTCAAGTCTGTAATGAACTCTTTTACTCTTGCATTACTATTTTCTATTATTAATCAATTATCAACATAAATGAGAATATAGACAAGGAAGAACAAAGAACCTGATTTAGAGTCATAAAGCCCCAACTTAGTAGAAGTTGTTCTGAACTTTTTGAGCCTTGGAGCTAGTTTTAGACCATACAATGCCTTGTGTAACTTGCATTCATGTTTAGGGTTTTTTGGGTCCACAATTCACATATCCTTCCAGTTGGAGCATATACCCATTTTATTTAATCTATCCATTCAAAAAGGAGTTATTTATATCCAGTTGCCTTTTTATGAAAGTTTTTTTTTATTCATATAAAAACGAGAGATCCCACCGCTTCAATTCACAGCCTCTAGTTATTGCTATGGGAACCGGTTGGGATGATTCTGATAGTTGAAGATTTAACAATAGGGATATGGGTTTCATAATTCATTGAAGTTTATTCTAGGATTTTGATGGAATCCTCAACGAATCTGGCTTTAACTTGTGCAGGGAATTATCTGAGTCCCAACGATATTTAATTCTGATAAGAGAGGAAAAAGGGTCCAATTTTTTTTTTAAGCAAGGCAGTAAATTCTTCATAACCTTTAACCTTTTCCAATGAGGAGAGGCTAGAGCTCCTTATCGATTATATACTGTATCTTTCCTCTTCATTTTCAATCTCCGTTTTTGGACTAGATGAGGTCAGTACCTTTTGGGAGTTAAGATCCCATCCTCTGCTCCAGTAACATAGGTGCCCTTGATTTTGTCCCATTCTTTTTGAGGACACCCACAGTAATCCTTAAGTCCTTTCCGAAGCTCTTCTGAAGTCATACAATGAGACTTGTATTGCAACATCCAAGTGAGACGATGGTACAAGTTTACAACCAACCAAGGAGTGATTGATACTTGATCATTTACAATCCAGTCACACTTATAATTACAAATAACCTGAGTTGAGTTTCAATCTGTGGTTGCTCAAATGATTGGAGTAACCTTAGTTCTCTTTATAAGATAGTTAAAGCCTTCTACATCTAAATCAAGCAGCGGAAGATATAGCAGATAGTTGTTGGGGCTGCTTAGGTGTAGTGGAAGTCTGCCTGGTTTAAGTCGTTGTTGAGATGGTTGCTTAGCTGGTCTATGATTTATTAAGCTGGGGATATGATTGTTTGAAATCGTTAGAGGGAAAATGCAAAATTATGAGACTAACTTAAGTTTTTATTGCTTTGTTAGTTTTTCCGGAAGGGGCCTCCTCGTCCCCTATCCTTAGGCTGTTATTTTGTCTTTTTGACTTCTTAATCAATTGTTTTATTATCCAAAAAAAAAAAAAAAAACTTAAGTTTTTTTTATTAATTTCAGAAGATAAATTAACAAAGAATTCTTCAACAAACTGCAATTGGATTAGATAATTTTGAATGTGATTAAAACTTGATACTGAAACATTATTGAGTTTGAAAATAACAATAACAATTAAAATGCTCTCACCAAGCCTAGACTTAAATCTTGGAAAATAAGTTCATTAACTATTTCCAGGTTTGCACCTGGAACTCTGTTCATTATGCTGAAGACAAGTTTACTTTCTGGTTAAATGAGATCGAACAACCTACTTTTATTCTGCAGAACATGATTGACTATTTGAAACAGCTGAGATACCGTGAAGTTTTGGGACCCTGGAGCAACCATGATTTGAAGATTAACAAACAATTGGCTTAATAATATTCTGAATTATGACTTCAAATTAAAAGCCTTTTCTTTGTTTGCATCAAGTAGTTCATAAATAAGAAAAAAGATTCCTTTAAGTAAAATTAACTTATAGTTTTCCTGTTGGAATAAATTGATGGACATTATATATCACATCATGTTTATGTTTATAATATTGGATCAAAATTGATACCAAGTGTTATGTAGAATGCATGATAGCAAGTTTTTTTGGAATCTTTTTCAGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTACAATGAAGAGTACAGGCTTCATGGAGCTCTTGAGGAAACTATTAAGTACTTTGAAGCATTGCTTAAAGCATTTCATTCTTTCCAATTAAATATTTGCACTTATTTTTTATGCCCAATAGCTCTCTTTGATCTTGGCTTTCTCTGTATACAACCCCCTCCGCACAGATTTGTCCTAATAGTCTCTACTTAGCTAGATACGACTGCCAAAGAAAAACCCTAAGAGCATGTTTCTAAACTTTTCAAGGCCATAATTATTTCCTATAAATAGAAAATATTAAGAGGCCTAAAATAAGCTACTAGACAAAGTATAATCTCATACCTGCCTTGCTTTGTAGTTACCTTCGACAACGTGCAGCAAAGGACAAAACATTTTCATATGAGGTATTTTCTTTTCTTTTTCTTTCTTTTTTTTCCATTTCTTCTTTTTATTTTTGTTATTGTCTCTTGATCATGGGAATTGATCGTGAATACTCTAAGAAAAATGGTATTTTTGGTTTGTTGATAATCTATCTTTTATATGCCTATACTTTCTTGCTTATTATTCCATGATATATTCACTTTCTTTTTCCATTAGCTCATGATGCTGGAATATTTTATATGGATTTATCCACACTCCACAGAAATAGATTAAAAAATATACATTAGCATTCTTCTATACTTGGAGAATGGGCCGATTTGTTTATTCTCTTTATGATTATTGAAATAAGCCCATTATTATTATTATTATTATTATTTTATAACTGGGAATCGGAGCTTTGCTCCACTATACCCACCCGCACTCGCCCCAAGGCCCAGACCTGAGAGACATTAAGGATTTTTTGTATTGAGCTCACGAGATGGTTTGAACCTGAGACATCTAAAGCCTTTATGACCAAGAGATCCCAAGCCCTTATTCAAAGCCCTCCTGGCAATAGGACATGAAAATTTCCCCATGATTAGGGTTCTTGAAAGATTTTTTGAACAATTAACGAATTATGCTACTTGAGTAGTGAAACCTTTGCTTTTCTTCTTCCGAAGATGAATTTATTTTCCAAACTCAAGAACCCTCACCACCCCCCTTCCCCATTTTTTTTCTTTTTTCAATTTCTCAATTTAAAAATGATAGGATATTCTATTAGTTTTATTGAATTAGTAATTAGGGAGACTGGATAGTTTAGAATTTAAATTACTGAGGATAATTAATTGGTTTTATCATTCTCTCTAAAATTAACATTTGTAAAGGACATAATGGTGTCTAGAACGTTTCTCTAAGTTTGGGCTTTTCTAATAAATAATTGATCATTGATCTTTGGACTACTCTACTAAAATAAGTAGAAAATTAACCAAAAGATATGATTGCTCATTGTCTCCCATCTCCGCCCATCTGAGGTAAAAGAATAAGAATATGTGATATAATGCATGAGGCTGACTTAGTCATTCAGGAACCTTCTTCGCCTGCTTTCCCTTTTGTGCTGAACTTTTAATCATCCAACATGAACACAGGTGGTAATTGTTGATGATGGCAGTGTTGATGCAACTAAAGGAGTAGCTTTCAATTTGGTTAGGAAGTACTCTGTAGAAAATGTGAGGGTTATACTTCTTGGGAAAAATCATGGAAAAGGGGAAGCTACTAGAAAGGTGAGTATGGGCTTTGCACTTTATTTGTAACTCTTATGTGATTTATCTTTACACCATTTCGTGATGGCAAGTTGGTGAGACTCCTTTTTAAAAAGCATTTTCAAGCAGGGAATGCTTCATTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAATATCACTGACCTAGAAAAACTTGAAAACCAGGTTTCGTCAAACCCTGTCTACTTTCAAGTGCAATCTTTTCAATAAAGTTAGCTTATAAAAGAAAAGGCCCCAAATTTTAAAAATGTGAACTAGTTACTCAAGACTTAATTTTTCTCCCCCAGATTCACGCAGAAGTAAAGAAGGAAGCTTCAAGAGATTCAAGTGTTAGAATATCTGATATTCCAATCACTGCATTTGGTTCTCGCGCCCATCTTGAGGAGAAGGCTCTGGCTACAGTTTAGTGTTGCTCATGCAGTTATCTCGGTTACTTGTTTGAAGTTGAACTTTTTTTTCATTTGATAGATCCTCTTACTTTTCAGAGGAAGTGGTACCGCAATTTCTTGATGAAGGGATTTCATCTCGTGGTTCTTCTGACAGCTGGTCCTGGAATACGGGATACACAGGTGATTCCCTTTTTAGTTTGCGTGAACATGCTCCTTCACGTGTATGTGATATTATCTCTGTGCTTATGCTGTGCAGTAATACAGATATAGTTGCTTTATACTAACTTCTGCCTGAAGCTGCTAAACTTCATTTTTTTAATACTTGCTTTCTATCACCGTGATCATATGCAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTTTTCACCAACATCCGTTTGAAGAGGTAAGATCCCTCCCCAACATATCTCTTGAAAGTTATAAGCACGACTAGGTCTAATTTAAACCATCATGACATACGGAGTAGATCCCGCTCTCTTTTACATGCATGCGACCATGACCACACCAGAAATAGTATGCTAAATCAGTAAGTACGAAATAGTGGAATTTATTGGATCCAAGCTGAGAGCAAAACCACAACACGAAAACTTTGCTGGCTTATTTTGTTTTCCCATTCTCTTTCTTAGTCCTGCCAATTGTCGGTGACTCCTTTTCAGCAATGTTGTGCTTGTGCTTCCATGAAATACTGAAACTTCCTTTGCAACCAGAATCGAACGGCTTAGCAATACTGAAACTTCCTTTTCAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTCCAATGCTTGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAGGTCCTTGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGACTGTCGGATACAGAACTGGAATTTGGAGAATTTCAATC

mRNA sequence

ATGGAGTATCCATGGACGATATTCTTCATTTTGATCAGCCTTTTTTTTACTTTCGCAGCAGGTTTGGTTTCTGCTATCATCCTCAAAGCTTATAGGAGGAGATTCAATCACGAGCACGTTGAAGCTCCTGCAATCTTGGAGGACCCAAACTCGTTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTACAATGAAGAGTACAGGCTTCATGGAGCTCTTGAGGAAACTATTAATTACCTTCGACAACGTGCAGCAAAGGACAAAACATTTTCATATGAGGTGGTAATTGTTGATGATGGCAGTGTTGATGCAACTAAAGGAGTAGCTTTCAATTTGGTTAGGAAGTACTCTGTAGAAAATGTGAGGGTTATACTTCTTGGGAAAAATCATGGAAAAGGGGAAGCTACTAGAAAGGGAATGCTTCATTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAATATCACTGACCTAGAAAAACTTGAAAACCAGATTCACGCAGAAGTAAAGAAGGAAGCTTCAAGAGATTCAAGTGTTAGAATATCTGATATTCCAATCACTGCATTTGGTTCTCGCGCCCATCTTGAGGAGAAGGCTCTGGCTACAAGGAAGTGGTACCGCAATTTCTTGATGAAGGGATTTCATCTCGTGGTTCTTCTGACAGCTGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTTTTCACCAACATCCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTCCAATGCTTGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAGGTCCTTGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGACTGTCGGATACAGAACTGGAATTTGGAGAATTTCAATC

Coding sequence (CDS)

ATGGAGTATCCATGGACGATATTCTTCATTTTGATCAGCCTTTTTTTTACTTTCGCAGCAGGTTTGGTTTCTGCTATCATCCTCAAAGCTTATAGGAGGAGATTCAATCACGAGCACGTTGAAGCTCCTGCAATCTTGGAGGACCCAAACTCGTTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATATGTCCTTGATCGTTCCTGCTTACAATGAAGAGTACAGGCTTCATGGAGCTCTTGAGGAAACTATTAATTACCTTCGACAACGTGCAGCAAAGGACAAAACATTTTCATATGAGGTGGTAATTGTTGATGATGGCAGTGTTGATGCAACTAAAGGAGTAGCTTTCAATTTGGTTAGGAAGTACTCTGTAGAAAATGTGAGGGTTATACTTCTTGGGAAAAATCATGGAAAAGGGGAAGCTACTAGAAAGGGAATGCTTCATTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAATATCACTGACCTAGAAAAACTTGAAAACCAGATTCACGCAGAAGTAAAGAAGGAAGCTTCAAGAGATTCAAGTGTTAGAATATCTGATATTCCAATCACTGCATTTGGTTCTCGCGCCCATCTTGAGGAGAAGGCTCTGGCTACAAGGAAGTGGTACCGCAATTTCTTGATGAAGGGATTTCATCTCGTGGTTCTTCTGACAGCTGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCAAGGAAGCTTTTCACCAACATCCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTCCAATGCTTGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAGGTCCTTGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGACTGTCGGATACAGAACTGGAATTTGGAGAATTTCAATC

Protein sequence

MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSIFDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGVAFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQIHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSKLGPWSILNMLWELALMTVGYRTGIWRISI
Homology
BLAST of MS003076 vs. NCBI nr
Match: XP_022133738.1 (dolichyl-phosphate beta-glucosyltransferase-like [Momordica charantia])

HSP 1 Score: 662.1 bits (1707), Expect = 2.4e-186
Identity = 328/329 (99.70%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV
Sbjct: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ
Sbjct: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180

Query: 181 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAEVKKEASRDSSVRISD+PITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAEVKKEASRDSSVRISDVPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300

Query: 301 LGPWSILNMLWELALMTVGYRTGIWRISI 330
           LGPWSILNMLWELALMTVGYRTGIWRISI
Sbjct: 301 LGPWSILNMLWELALMTVGYRTGIWRISI 329

BLAST of MS003076 vs. NCBI nr
Match: XP_038889980.1 (dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida])

HSP 1 Score: 599.7 bits (1545), Expect = 1.5e-167
Identity = 292/327 (89.30%), Postives = 309/327 (94.50%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI FILISL    A GL+SA+I++AYRRRFNH+HVEAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFILISLALCIAVGLISAVIIEAYRRRFNHQHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRLHGALEETINYL+QRAAKDK++SYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSYSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHA VKKEASRD S+RISDIPI AFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAVVKKEASRDPSIRISDIPIAAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSA RKLF+NIRLKRWCFDVELVYLCK+FGIPM+EVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAGRKLFSNIRLKRWCFDVELVYLCKFFGIPMIEVSVNWSEIPGSK 300

Query: 301 LGPWSILNMLWELALMTVGYRTGIWRI 328
           L P SILNMLWELALM+VGYRT IWRI
Sbjct: 301 LSPRSILNMLWELALMSVGYRTRIWRI 327

BLAST of MS003076 vs. NCBI nr
Match: KAG7033054.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 294/330 (89.09%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI F  ISL  +  A L+SAIIL+AYRRRFNH H+EAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYLRQRAAKDK+FSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLRQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHA--EVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHA   VKKE SRD S+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVVVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 329
           SKL P SILNMLWELALM+VGYRTG WRIS
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIS 330

BLAST of MS003076 vs. NCBI nr
Match: KAG6602376.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590.5 bits (1521), Expect = 8.9e-165
Identity = 293/330 (88.79%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI F  ISL  +  A L+SAIIL+AYRRRFNH H+EAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYLRQRAAKDK+FSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLRQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           I   A VKKE SRD S+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IQAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 329
           SKL P SILNMLWELALM+VGYRTG WRIS
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIS 330

BLAST of MS003076 vs. NCBI nr
Match: XP_022953750.1 (dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 590.5 bits (1521), Expect = 8.9e-165
Identity = 292/330 (88.48%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI F  ISL  +  A L+S IIL+AYRRRFNH H+EAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYL+QRAAKDK+FSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IH  A VKKE SRD S+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 329
           SKL P SILNMLWELALM+VGYRTG WRIS
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIS 330

BLAST of MS003076 vs. ExPASy Swiss-Prot
Match: Q9Y673 (Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.7e-68
Identity = 136/292 (46.58%), Postives = 187/292 (64.04%), Query Frame = 0

Query: 37  HEHVEAPAILEDPNSLKRVPCPSIFDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRA 96
           H H E    L      ++   PSI+D   K +S++VP+YNEE RL   ++E ++YL +R 
Sbjct: 38  HRHEEEKFFLNAKG--QKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQ 97

Query: 97  AKDKTFSYEVVIVDDGSVDATKGVAFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSR 156
            +D  F+YEV++VDDGS D T  VAF   +KY  + VRVI L KN GKG A R G+  SR
Sbjct: 98  KRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSR 157

Query: 157 GELLLMLDADGATNITDLEKLENQIHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKAL 216
           GE +LM DADGAT   D+EKLE  ++         D     + + I A GSRAHLE++++
Sbjct: 158 GEKILMADADGATKFPDVEKLEKGLN---------DLQPWPNQMAI-ACGSRAHLEKESI 217

Query: 217 ATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELV 276
           A R ++R  LM GFH +V      GIRDTQCGFK+FTR AA + F+++ ++RW FDVEL+
Sbjct: 218 AQRSYFRTLLMYGFHFLVWFLCVKGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELL 277

Query: 277 YLCKYFGIPMLEVSVNWSEIPGSKLGP-WSILNMLWELALMTVGYRTGIWRI 328
           Y+ ++F IP+ E++VNW+EI GSKL P WS L M  +L  + + Y TG WR+
Sbjct: 278 YIAQFFKIPIAEIAVNWTEIEGSKLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of MS003076 vs. ExPASy Swiss-Prot
Match: Q9VLQ1 (Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=wol PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.1e-67
Identity = 148/329 (44.98%), Postives = 197/329 (59.88%), Query Frame = 0

Query: 5   WT----IFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 64
           WT    + F L+S     A  + + ++ K         H +    L DP+++K V  PS+
Sbjct: 2   WTCLCQLCFYLLSTLAVAALSIAALVLYKTKPYPNIKRHKDEETFL-DPHTIKTVTFPSL 61

Query: 65  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 124
            D     +S+IVPAYNEE RL   L+E + +L Q++A    F+YEV++V DGS DAT  V
Sbjct: 62  EDSPSLELSVIVPAYNEEQRLPSMLDECLAFLEQKSAGTPNFTYEVIVVSDGSQDATVSV 121

Query: 125 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 184
           A    +K+  E VRV+ L +N GKG A R GML +RG  LL  DADGAT   D +KLE  
Sbjct: 122 ALGYSKKHGAEKVRVLELIENRGKGGAVRMGMLSARGRNLLFADADGATKFPDYDKLE-- 181

Query: 185 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 244
           +  +      RD  +        A GSRAHLE  A+ATR ++R  LM GFH +V L A  
Sbjct: 182 VALKQLAPEWRDDGI--------AIGSRAHLENDAIATRSFFRTILMHGFHFLVWLFAVR 241

Query: 245 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 304
            IRDTQCGFK+FTR+ ARKLFT++ ++RW FDVEL+YL +   +PM EV+V W+EI GSK
Sbjct: 242 SIRDTQCGFKLFTRTTARKLFTSLHVERWAFDVELLYLAENLKLPMSEVAVRWTEIDGSK 301

Query: 305 LGP-WSILNMLWELALMTVGYRTGIWRIS 329
           L P WS L M  +L ++ V Y  G WRI+
Sbjct: 302 LTPFWSWLQMGRDLFMIWVRYLVGAWRIA 319

BLAST of MS003076 vs. ExPASy Swiss-Prot
Match: Q9DB25 (Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.8e-66
Identity = 132/271 (48.71%), Postives = 176/271 (64.94%), Query Frame = 0

Query: 58  PSIFDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDAT 117
           PSI+D   K +S++VP+YNEE RL   ++E +NYL +R   D TF+YEV++VDDGS D T
Sbjct: 57  PSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALNYLEKRQKHDCTFTYEVIVVDDGSEDQT 116

Query: 118 KGVAFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKL 177
             VA    +KY  + VRVI L +N GKG A R G+  SRGE +LM DADGAT   D+EKL
Sbjct: 117 SKVALKYCQKYGSDKVRVITLVRNRGKGGAVRMGVFSSRGEKILMADADGATKFPDVEKL 176

Query: 178 ENQIHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLT 237
           E  +          D       + I A GSRAHLE++++A R ++R FLM GFH +V   
Sbjct: 177 EKGL---------SDLQPWPEQMAI-ACGSRAHLEKESIAQRSYFRTFLMYGFHFLVWFL 236

Query: 238 AGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIP 297
              GIRDTQCGFK+ TR AA + F+++ ++RW FDVEL+Y+ +   IP+ EV+VNW+EI 
Sbjct: 237 CVKGIRDTQCGFKLLTREAAARTFSSLHIERWAFDVELLYIAQCLQIPIAEVAVNWTEIE 296

Query: 298 GSKLGP-WSILNMLWELALMTVGYRTGIWRI 328
           GSKL P WS L M  +L  + + Y TG WR+
Sbjct: 297 GSKLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of MS003076 vs. ExPASy Swiss-Prot
Match: A2DZE8 (Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=5722 GN=ALG5A PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-59
Identity = 135/329 (41.03%), Postives = 196/329 (59.57%), Query Frame = 0

Query: 9   FILISLFFTFAA-GLVSAIILKAYRRRFNHEHVEAP---------AILEDPNSLKRVPCP 68
           F+ I  F   AA GLV A+++         + ++ P          I   PN  ++VP P
Sbjct: 6   FVQILFFLGVAAVGLVVAVMIANADDTTLFDRMQLPDGDPNKLNYYIQPAPNGNEKVPFP 65

Query: 69  SIFDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATK 128
           +IFDPA  Y+SL+VPAYNEE RL   L+ET+NYL+ R  KDK+F++E+VIV+DGS D TK
Sbjct: 66  TIFDPASVYLSLVVPAYNEEKRLPKMLDETLNYLKSREEKDKSFTWEIVIVNDGSKDKTK 125

Query: 129 GVAFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLE 188
            V  N  ++Y   N+ ++    N GKG A + G LH RGEL+LMLDADGAT I D E LE
Sbjct: 126 EVVLNYAKEY--PNIFLLNQPVNMGKGAAIQAGCLHVRGELVLMLDADGATKIDDFEVLE 185

Query: 189 NQIHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 248
            +I + +K   + + ++ I        GSRA  E+   A R   R FL  G H +++L+ 
Sbjct: 186 KEIKSLMK---TTNQAIVI--------GSRAQNEK---AKRTPLRKFLSIGMHTLIVLSG 245

Query: 249 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 308
             GIRDTQCGFK+FTR + + +F N  ++RWC D E++ + +  G+ + E+ V W+EI G
Sbjct: 246 VHGIRDTQCGFKLFTRESCKMIFMNQHVQRWCCDPEILVIARRLGMKISELPVEWNEIDG 305

Query: 309 SKLGPWSILNMLWELALMTVGYRTGIWRI 328
           SK+    ++ M  +L  + + +R G W+I
Sbjct: 306 SKMKISGMIKMATDLIKIAIFHRVGAWKI 318

BLAST of MS003076 vs. ExPASy Swiss-Prot
Match: Q54J42 (Dolichyl-phosphate beta-glucosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg5 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.3e-59
Identity = 118/292 (40.41%), Postives = 180/292 (61.64%), Query Frame = 0

Query: 38  EHVEAPAILEDPNSLKRVPCPSIFDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAA 97
           E  + P+++E+ N +  +           Y+S+IVPAYNE+ RL   L++ I +L +++ 
Sbjct: 57  EQHQFPSLVENKNPIDNI-----------YLSVIVPAYNEQIRLPSMLDDAIKFLNEKSK 116

Query: 98  KDKTFSYEVVIVDDGSVDATKGVAFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRG 157
           KD  FSYE++I+DDGS D+T  +  + + K    N+R++ L +N GKG A ++G+L SRG
Sbjct: 117 KDLKFSYEIIIIDDGSKDSTAKLVTSYIEKQPSSNIRLLKLKQNRGKGGAVKRGILCSRG 176

Query: 158 ELLLMLDADGATNITDLEKLENQIHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKAL- 217
           +  LM+DADGAT   D  ++E+ +H            +  +D+ I   GSR+HL +  L 
Sbjct: 177 KYCLMVDADGATEFKDFNRVEDIMH-----------KIEKNDLGIVC-GSRSHLVDSDLV 236

Query: 218 ATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELV 277
           A R + RN LM GFH+ V      GI+DTQCGFK+FTR  AR++F  + ++RW FDVE++
Sbjct: 237 AKRSFLRNILMYGFHIFVQTLCVKGIKDTQCGFKLFTRETARRIFPTLHIERWAFDVEIL 296

Query: 278 YLCKYFGIPMLEVSVNWSEIPGSKLGPW-SILNMLWELALMTVGYRTGIWRI 328
           YL +   IP+ EV+VNW+EI GSKL P+ S + M  ++  +   Y  GIW+I
Sbjct: 297 YLAQKLNIPIAEVAVNWTEIDGSKLDPFSSSIQMAKDIVRIRFRYLLGIWKI 325

BLAST of MS003076 vs. ExPASy TrEMBL
Match: A0A6J1C019 (Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111006250 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.2e-186
Identity = 328/329 (99.70%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV
Sbjct: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ
Sbjct: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180

Query: 181 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHAEVKKEASRDSSVRISD+PITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAEVKKEASRDSSVRISDVPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300

Query: 301 LGPWSILNMLWELALMTVGYRTGIWRISI 330
           LGPWSILNMLWELALMTVGYRTGIWRISI
Sbjct: 301 LGPWSILNMLWELALMTVGYRTGIWRISI 329

BLAST of MS003076 vs. ExPASy TrEMBL
Match: A0A6J1GQI8 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456187 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.3e-165
Identity = 292/330 (88.48%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI F  ISL  +  A L+S IIL+AYRRRFNH H+EAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYL+QRAAKDK+FSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IH  A VKKE SRD S+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 329
           SKL P SILNMLWELALM+VGYRTG WRIS
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIS 330

BLAST of MS003076 vs. ExPASy TrEMBL
Match: A0A6J1JRS3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487753 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.7e-165
Identity = 294/330 (89.09%), Postives = 308/330 (93.33%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYP TI FILISL  +  A L+SAIIL+AYRRRFNH H+EAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPRTILFILISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYL+QRAAKDK+FSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IH--AEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IH  A VKKE SRD S+RISDIPITAFGSRAHL+EKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLKEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 329
           SKL P SILNMLWELALM+VGYRTGIWRIS
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGIWRIS 330

BLAST of MS003076 vs. ExPASy TrEMBL
Match: A0A0A0KSA3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G517110 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.1e-163
Identity = 283/327 (86.54%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEYPWTI FI ISL   F A L+S +I++AY+RRFN EH+ APAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFIFISLALCFPAALLSTLIIEAYKRRFNREHLGAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRL GALEETINYL+QRAAKDK+FSYEV+I+DDGS D TKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVENVRVILLG+NHGKGEA RKGMLHSRGE+LLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHA VKKE SRDSSVR+SDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP
Sbjct: 181 IHAVVKKETSRDSSVRVSDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGIPM+EVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LGPWSILNMLWELALMTVGYRTGIWRI 328
           L P SILNMLWELALM++GY+TG+W+I
Sbjct: 301 LSPQSILNMLWELALMSIGYKTGLWKI 327

BLAST of MS003076 vs. ExPASy TrEMBL
Match: A0A1S4E276 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498152 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 281/327 (85.93%), Postives = 302/327 (92.35%), Query Frame = 0

Query: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60
           MEY WTI FI ISL   FAA ++SA+I++A++RRFN EHVEAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYLWTILFIFISLALCFAAAIISALIIEAFKRRFNREHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120
           FDPAEKYMSLIVPA+NEEYRLHGALEETINYL+QRAAKDK+FSYEV+I+DDGS D TKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180
           AFN VRKYSVE VRVILLG+NHGKGEA RKGMLHSRGE+LLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNFVRKYSVEKVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAEVKKEASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGP 240
           IHA  KKE SRDSS++ISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLL AGP
Sbjct: 181 IHAVAKKETSRDSSIKISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLIAGP 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPGSK 300
           GIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVEL YL KYFGIPM+EVSVNWSEIPGSK
Sbjct: 241 GIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELFYLSKYFGIPMIEVSVNWSEIPGSK 300

Query: 301 LGPWSILNMLWELALMTVGYRTGIWRI 328
           L P SILNMLWELALM++GY+ GIWRI
Sbjct: 301 LSPRSILNMLWELALMSIGYKIGIWRI 327

BLAST of MS003076 vs. TAIR 10
Match: AT2G39630.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.0e-131
Identity = 227/329 (69.00%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 5   WTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSIFDPA 64
           ++++ +LI LF     G +S ++ +A+RRR ++  VE    L+DP S+K +PCP I DPA
Sbjct: 10  FSLWLLLIVLF-----GFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHITDPA 69

Query: 65  EKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGVAFNL 124
           EKY+SLIVPAYNEE RL  ALEET++YL+ RA++DK+FS+EVVIVDDGSVD TK VAF+ 
Sbjct: 70  EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 129

Query: 125 VRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQIHAE 184
           +RKY+++N+RVI LGKN GKGEA RKGMLHSRG+LLLMLDADGAT +TDLEKLENQI+A 
Sbjct: 130 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQINAV 189

Query: 185 VKKE------ASRDSSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 244
            ++E      AS+D   +I D+ ++AFGSRAHLEEKALATRKWYRNFLMKGFHLVVLL A
Sbjct: 190 AREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLAA 249

Query: 245 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 304
           GPGIRDTQCGFKMFTR+AAR+LFTN+ LKRWCFDVELVYLCK F IPM+E+SV WSEIPG
Sbjct: 250 GPGIRDTQCGFKMFTRAAARRLFTNVHLKRWCFDVELVYLCKRFNIPMVEISVKWSEIPG 309

Query: 305 SKLGPWSILNMLWELALMTVGYRTGIWRI 328
           SK+   SI NMLWELALM+VGYRTG+W+I
Sbjct: 310 SKVSMLSIPNMLWELALMSVGYRTGMWKI 333

BLAST of MS003076 vs. TAIR 10
Match: AT2G39630.2 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 136/220 (61.82%), Postives = 175/220 (79.55%), Query Frame = 0

Query: 5   WTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSIFDPA 64
           ++++ +LI LF     G +S ++ +A+RRR ++  VE    L+DP S+K +PCP I DPA
Sbjct: 10  FSLWLLLIVLF-----GFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHITDPA 69

Query: 65  EKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGVAFNL 124
           EKY+SLIVPAYNEE RL  ALEET++YL+ RA++DK+FS+EVVIVDDGSVD TK VAF+ 
Sbjct: 70  EKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRVAFDF 129

Query: 125 VRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQIHAE 184
           +RKY+++N+RVI LGKN GKGEA RKGMLHSRG+LLLMLDADGAT +TDLEKLENQI+A 
Sbjct: 130 IRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQINAV 189

Query: 185 VKKE------ASRDSSVRISDIPITAFGSRAHLEEKALAT 219
            ++E      AS+D   +I D+ ++AFGSRAHLEEKALAT
Sbjct: 190 AREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALAT 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133738.12.4e-18699.70dolichyl-phosphate beta-glucosyltransferase-like [Momordica charantia][more]
XP_038889980.11.5e-16789.30dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida][more]
KAG7033054.11.1e-16589.09Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
KAG6602376.18.9e-16588.79Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
XP_022953750.18.9e-16588.48dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9Y6734.7e-6846.58Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1... [more]
Q9VLQ13.1e-6744.98Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 G... [more]
Q9DB255.8e-6648.71Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=... [more]
A2DZE82.4e-5941.03Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=57... [more]
Q54J425.3e-5940.41Dolichyl-phosphate beta-glucosyltransferase OS=Dictyostelium discoideum OX=44689... [more]
Match NameE-valueIdentityDescription
A0A6J1C0191.2e-18699.70Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1GQI84.3e-16588.48Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JRS35.7e-16589.09Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KSA33.1e-16386.54Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S4E2761.0e-16185.93Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
Match NameE-valueIdentityDescription
AT2G39630.14.0e-13169.00Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT2G39630.21.6e-7161.82Nucleotide-diphospho-sugar transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001173Glycosyltransferase 2-likePFAMPF00535Glycos_transf_2coord: 69..183
e-value: 4.3E-21
score: 75.4
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 53..299
e-value: 6.9E-31
score: 109.7
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 65..309
NoneNo IPR availablePANTHERPTHR10859GLYCOSYL TRANSFERASEcoord: 13..327
NoneNo IPR availablePANTHERPTHR10859:SF108BNAA03G18660D PROTEINcoord: 13..327
IPR035518Dolichyl-phosphate beta-glucosyltransferaseCDDcd04188DPG_synthasecoord: 70..300
e-value: 1.96784E-101
score: 295.245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003076.1MS003076.1mRNA