MS003041 (gene) Bitter gourd (TR) v1

Overview
NameMS003041
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMethionine--tRNA ligase
Locationscaffold234: 143524 .. 148788 (+)
RNA-Seq ExpressionMS003041
SyntenyMS003041
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGGTTTTTTTTTTCTTCTTCTTAATCTATAATATTCATGCTGTTCGTTTAATTTTTGCTTTTACAAATTAGATGGGGCGTTTGTTTATCGCTTGCTTGAATAAGCTCTGAGTAGTTTTTGTTCCTCTCATTTGTTGGTTATTTTTTGTCGGTACTTGGTAGCTGAAATCGAATATATTGTCACCATAATTGACTTCTCTACCTGTTTTCTTTGTATGTTTCATTATATCACAGCCTGTATTTGAGAGCAAGAACGTTGAGAGTTTCAATTCTCACACCTCTCTCTTTCTGATATCTGCTGCTTACGATTTTTAATAACTGAAATTTTGGAATACAGTTTTACCATTAATCGCTCCTAACTGAATTAAAAAAGTTAAGTCCCTACCTTGCCGCATGGGAGTGTGTAGCAAGAATTAATTTACTTAGGGTCTAGCTGAAATTTGATTGTGTAGCAAGATGCTTTTAACAATTTGTTTTATAGTTGGCTTTGTTTAGAAGAACTGAACTTTAAGTTTACAAATTAAACAAATATATCATTTGTGCAAATTAGAACCAATGCTCCTCAGTTACTGTTGCAGTCTTCCTCTAAGGGATATTATAAATGGTCAATAAGTTTTGGTATGTCCTATAGAGTTGATTTCTCGTATAGCTTACTAGTTGGCAAATTCCGGTGTTTGATTAGAACTATTTCACCATGCTTATAGCCAGTTTCCTTGGAATATTTTGCTAATTTTTGGCTAACCTATGGTGTCAAATATAATGATAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATGTAGTCCAAACACATCACAGGATTTTCTTCTCAGTTTGCTGTAATTCATTTATTAGATATTTGAAATTATTCATAGTATTTTACATTCTTATTCTTTTTCTTCCTTTTTTCCCCTTTGCAGCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGTGTTCTTTTTACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTTTTGTCTTCCTCCCTTTACCCATTTGAAAAATTTGGATTTGGATTCATATGATAATTGTGCGCCAAATTTCGTTTTATTGTTAACATTGCTTCTTTCCTCAATTATGATAATATATTGACCTAACTCTGCTTTTCCAATAACTATTTTCCTGGGGTATGGATGCATTTAACAGTAGTTATTTCTACAATTTTGAAGGTATAGTATGCACGAATTCTCTTGAGTTTTGTAAGAGCTTCCATTTATAAAACTGCAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGACTATAGGGGATTTTCTACTTGTGTGTGTGTTTATGTGTGAAATTTGTTACTTCTCTCCTAAAGTACTGCATTACATAATTTTCTTTAAAAAGTTCCGGATCAAATATATTTGTGTCGGAACTGATGTTAAATCCAAGAGAAACAAATGGCTTGGAGCATACAAAAAGGCGTTCAAATCAGCTGAACTTTAAAACAATGGGAAGTAAAAAAAAAACTATTAGTAAGAAGCCCCCAAAGGAAACCTGAGAAACAGTGTGATTCCAAACCCTAATTCTGATAGAACTTTTATTGTGATACCAATAAACAAGAAGTTGATTGACTTATTTAATGATCAATTGTAGTTGTAGATTGACTTGCTAAGAGTGATAATATAGAACTCCATGCATATATACCATAAATTATTATTATTCAAGCCTTCAAGGTCTACCATTTAGTATGACCTAAACTAAATGTAATTCTTCTAATTGCTCATCAAATCTCTTTGATCTAATTTGATATTGTATTTATATACGAATTTTTTCTTACAACAGAATGTCAGTTCTATTATTTGTTACAATTTTTTTCCACCTTGCCGATTGTGAATCATCCTTTGATTAATTCAGCACCATATCTTCCAAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGGTAATATCTTCCCTATGTTTTTGTAATGTCTTATATATCGTGACTTCTTGAGCCTTTATGATTATGGCCAACTTTTTTATTTATTTACTTCTTAGCTCTTCATCACAATCCACTACAAACATACGGTTTATGTAAATTTGCAACAACTATGCACCATTTTGCTATAGTCTGTCTTTAATCTTAACTATATGAGAATTCCATAATGTAGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGTAAGAGACACATTCCTGTTCTTTGTACGTAGACTTATCTGGCCCTAGCTGAAAACTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATCTGTTTTTCAGATTATGTCTTTTCTACTACATTTCTCTTGAATATAGCAAGAAGTGAAATTATGAATCAACTCTAGTTTCTGTGTTATTATTGTATTCTGGAGGTTCTGATGATTGTTTCCCTTTTCTTTTCTTTACTGAGTGCAAGCTGAGTCTGTAGGATGCCGATCTGATGTTTTTGTCATTCTTTTTTGTAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGTCATGGGTTCTTGACAAAGGTATTATGTTTGGCCTCCTAAATTTACTACAAAGGAATTTAGTTGGCCATGATATGTCGAACTCTAATTGATGCAGAAGAGAATTAATATTTGTCAAATTGACGAAGTCTATAAAGAATCAAATTAACTTGTCACCATTGCTATAGTATATAATGTTAATATTATTGCATCTTGTGAATTATAAAGGGTCTTGATTGTCTTGTGAATCTTATTGAATCTCTTGGAGTTGCAACATTCAATGCCCCTCCTAACCAAACACTTCCAACAATTGGATTCCTTGACTTTTAGCTTCATGTTCACAAAGAAGCTTGAACCAGTGAATCTCTGCAGAGGCACAAATACTTCGTTATTATTTCATGCAGCAACTCTTTTTGCTTTAACATTATAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGTATTTATAAACCGTAATTCCTTCCGTTTGGAAAATTACAGCACTTTTGCTGTACACTTCTGTTTTTTCTTTTTTAATAATCGGGAGTCGAGCCTCCCTAAACTTGGGGGCACCTGTGGGTTGTGCCCACTCCAAGGACCATATTTGGGAGACATCAAGAGTTTTTGGTATTAAGCTCATATTTTCTAGGCCGGTATGATCCAGAGATCCAAAGCTTTTGCCAACGAGGACCACCACTTGGGGGCTTGAGGGAGATTTCTGTTTGACACAACATAATTCTCGATGTTTCTTCTGCAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTAACAGTTTGCTCTGAATTTACGTTGTTGTTTGTTATTTGAGATGGCGTGGAATTAGTAGACCCTGAAATCTTCTCTTGAATCTCTATTGTGAAAGGCACTGAATCCATCGTGGTGCTGATATTGTCTGTGTGTCCCTCCATTTATGACTTCGTGATTGACATGTTCATGAATTTTTGCAAACTATGTTGATTTTATTTGTGTGCCTGGTAGGTTGCGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGTTGTTCAGTTCAAGAACCCATCTTTCTTCACGATCATGATCTCGGACGCGAATTCTCGATCAACTTTTCCTTCCAACATACAAGAAACCATTCTCTGACAACCATCTGCCTTGGTTTTCTCAACAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTACACTATCCCCAGTGACTCCATGCTTAAGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGGTCTGTTTCTCTTTTCACACTTGTGTGTTTGATATCAGCTCTTTTTATTACTAGTGACTGATAATGTGGTTTTTGGATGTAGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCC

mRNA sequence

ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGTCATGGGTTCTTGACAAAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTTGCGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTACACTATCCCCAGTGACTCCATGCTTAAGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCC

Coding sequence (CDS)

ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGTCATGGGTTCTTGACAAAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTTGCGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTACACTATCCCCAGTGACTCCATGCTTAAGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCC

Protein sequence

MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKPVKSKKKIAQGVAEA
Homology
[Close]
BLAST of MS003041 vs. NCBI nr
Match: XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 610/616 (99.03%), Postives = 611/616 (99.19%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSS--LNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARINSSIQSSLWLLNPLQSS  LNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELV KARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVT 540
           LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAV LSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540

Query: 541 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           PCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 615
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616

[Close]
BLAST of MS003041 vs. NCBI nr
Match: XP_038891037.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 588/614 (95.77%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SLWLLNP QSSLNL    +LRTRL+FY+NLL  PS S+SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLWLLNPCQSSLNLK-PTHLRTRLHFYQNLLSLPS-SSSSSKRAIFCTA 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
           A++ NSH++GAAT+PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT
Sbjct: 61  ATSFNSHDNGAATNPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS+PSEHCD ISEAYKTLWKDLDVAYD+FIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSSPSEHCDVISEAYKTLWKDLDVAYDRFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           +VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASR+EDNYFFALSKYQ 
Sbjct: 181 RVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRREDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NL+R VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLERVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVGKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLL+KNCQSTLVVDSTAAA GN FKDKVEEL+ KARNHYE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLQKNCQSTLVVDSTAAAGGNVFKDKVEELIVKARNHYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGAS+DAAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASTDAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+S+DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPKKP
Sbjct: 541 LSWRIYAQLGYSEDQFNAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQGVAEA
Sbjct: 601 VKSKKKIAQGVAEA 612

[Close]
BLAST of MS003041 vs. NCBI nr
Match: XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 583/614 (94.95%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SLWLLNP  SSLNL    + RTRL+F +NLL  PS+  SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLK-PTHFRTRLHFCQNLLSFPSH--SSSKRAIFCTS 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
            SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFITGT
Sbjct: 61  TSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           KVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ 
Sbjct: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NLQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV KARN+YE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PKKP
Sbjct: 541 LSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQ VAE+
Sbjct: 601 VKSKKKIAQAVAES 611

[Close]
BLAST of MS003041 vs. NCBI nr
Match: XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 560/615 (91.06%), Postives = 582/615 (94.63%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIFCT 60
           MAARIN+SIQ+SLWLLNPLQSS NL    +LRTR NFY+NLL +P S S+SSSKRAIFC+
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKAT-HLRTRFNFYQNLLSAPSSCSSSSSKRAIFCS 60

Query: 61  YASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120
            ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG
Sbjct: 61  SASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120

Query: 121 TDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 180
           TDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKEFY
Sbjct: 121 TDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFY 180

Query: 181 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQ 240
           S VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ
Sbjct: 181 SNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQ 240

Query: 241 GLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300
            LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVWFD
Sbjct: 241 RLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300

Query: 301 ALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360
           ALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV
Sbjct: 301 ALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360

Query: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 420
           FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFINIV
Sbjct: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIV 420

Query: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSL 480
           NAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELVAKARNHYE LSL
Sbjct: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEELSL 480

Query: 481 SSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTP 540
           SSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ LSPVTP
Sbjct: 481 SSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTP 540

Query: 541 CLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKK 600
            LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PKK
Sbjct: 541 RLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKK 600

Query: 601 PVKSKKKIAQGVAEA 615
           PVKSKKKIAQ VAEA
Sbjct: 601 PVKSKKKIAQKVAEA 614

[Close]
BLAST of MS003041 vs. NCBI nr
Match: KAA0066020.1 (methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 583/614 (94.95%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SL LLNP  +SLNL    + RTRL+FY+NLL   SY  SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK-PTHFRTRLHFYQNLLSFASY--SSSKRAIFCTT 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
            SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI ITGT
Sbjct: 61  TSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           KVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ 
Sbjct: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV KARNHYE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPKKP
Sbjct: 541 LSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQGVAEA
Sbjct: 601 VKSKKKIAQGVAEA 611

[Close]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 1.1e-264
Identity = 462/622 (74.28%), Postives = 524/622 (84.24%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIFCT 60
           MAARIN+S+ ++L  L P  + LN       R    F + L   P YS  SS KRA++CT
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKPFSFRRNSFRFSKKL---PYYSQFSSGKRALYCT 60

Query: 61  YASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120
            +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFITG
Sbjct: 61  SSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFITG 120

Query: 121 TDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 180
           TDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKEFY
Sbjct: 121 TDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFY 180

Query: 181 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQ 240
           ++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSKYQ
Sbjct: 181 ARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKYQ 240

Query: 241 GLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300
             LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWFD
Sbjct: 241 KPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFD 300

Query: 301 ALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360
           ALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKMV
Sbjct: 301 ALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMV 360

Query: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 420
           FGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI IV
Sbjct: 361 FGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKIV 420

Query: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSL 480
           NAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LSL
Sbjct: 421 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSL 480

Query: 481 SSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTP 540
           SSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAV LSPV P
Sbjct: 481 SSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAP 540

Query: 541 CLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE---- 600
           CLS RIY+QLG+++DQF + TW++TKWGGLK GQVM   +PVFARIE +   +EDE    
Sbjct: 541 CLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKKPK 600

Query: 601 VPKKPVKSKKKIAQ---GVAEA 615
           V KK  K+K K+ +    VAEA
Sbjct: 601 VGKKTGKAKVKVVEQTPTVAEA 616

[Close]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match: Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.9e-159
Identity = 277/521 (53.17%), Postives = 358/521 (68.71%), Query Frame = 0 

Query: 76  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 135
           F +TTPLYYVN  PH+GSAYTT+ AD +ARF+RL G  V+ ITGTDEHG+KI   A AQ 
Sbjct: 9   FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68

Query: 136 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 195
             P  HCD     +K LW+ L++ YD+F RTTDP+H AIVK+F+ +V   GDIY A  +G
Sbjct: 69  LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128

Query: 196 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 255
            YCV CEE+K++++LL +N CP+H    A  R E+NYFF LS+YQ  LEE   Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188

Query: 256 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 315
           P+ R NEV +++  GL+DFSISR +++WG P+PND   TIYVWFDALLGY++AL +  ++
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248

Query: 316 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 375
            NL  A+   WP +LHLIGKDILRFHAVYWPAMLMSA L +P  VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308

Query: 376 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 435
           SLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368

Query: 436 LGLLKKNCQST-LVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLSSACEAVLEIGNAG 495
           LG++KK CQ     V +T  A  N  K     L  +  + YE LS + ACEA+  +  AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428

Query: 496 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLGFSK 555
           N Y+D+ APW LFKQG  S       L  +LE++R+ A  LSP+ P LS +IY QLGF+ 
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488

Query: 556 D-------QFEAATWN-ETKWGGLKAGQVMAPPNPVFARIE 587
           D         +A  +N    WG L A Q + P  P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527

[Close]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match: Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 4.1e-158
Identity = 279/523 (53.35%), Postives = 365/523 (69.79%), Query Frame = 0 

Query: 72  ATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 131
           A   F +TTPLYYVN  PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI  +A
Sbjct: 7   AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66

Query: 132 AAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRA 191
            + G AP E CD I  ++ +LW+ L++ YD+F RTT  +H+AIV EF+++V   GDIY+ 
Sbjct: 67  ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126

Query: 192 DYEGLYCVNCEEYKDEKELLANNCCPIHL-KPCASRKEDNYFFALSKYQGLLEETLTQNP 251
             +G YCV+CEE+K+E+ELL  N CPIH+ K    R E NYFF LSKYQ  LEE    +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186

Query: 252 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 311
            F+QP  R NEV +++  GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246

Query: 312 NGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 371
              +  L+ A+   WP +LHLIGKDILRFHAVYWPAML+SAGL LP  VFGHGFLTKDG 
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306

Query: 372 KMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNL 431
           KMGKSLGNT++P  LVQ++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366

Query: 432 LNRTLGLLKKNCQS-TLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLSSACEAVLEI 491
           LNRTL ++KK C +  L + +      N+ K    +L AK +  YE L+ + AC  VL +
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSL 426

Query: 492 GNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQL 551
             A N ++D+QAPWSL+KQ           L  +LE++R+ A  LSPV P +S  IY QL
Sbjct: 427 VQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQL 486

Query: 552 GFS---KDQFEAATWN-ETKWGGLKAGQVMAPPNPVFARIEQS 589
           GF     +Q +   +    +WG L   Q +  P P+F RIEQ+
Sbjct: 487 GFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQT 527

[Close]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 5.4e-150
Identity = 264/518 (50.97%), Postives = 347/518 (66.99%), Query Frame = 0 

Query: 76  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 135
           F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI   A  +G
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64

Query: 136 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 195
            +P  HCD I+  ++ LW+ L++ YD+F RTT P+H AIV EF+ +V  NGDIY    +G
Sbjct: 65  LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124

Query: 196 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 255
            YCV CEE+K+E+ELL    CPIH+      R E NYFF LSKYQ  L +   ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184

Query: 256 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 315
           P  R NEV S+I  GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E   +
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244

Query: 316 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 375
             L  A+ + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304

Query: 376 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 435
           SLGNTL+P  LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364

Query: 436 LGLLKKNCQSTLV-VDSTAAAEGNSFKDKVEELVAKARNHYEGLSLSSACEAVLEIGNAG 495
           L +  K     +  V   A    +  +   E L       Y  L+    C+  L +  AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424

Query: 496 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLGFSK 555
           N ++DE+APW  ++ G   + A A+ L  +LE++R++A  L+P+ P LS  IY QLG+S 
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYS- 484

Query: 556 DQFEAATW------NETKWGGLKAGQVMAPPNPVFARI 586
            +F  +         + +WG L A Q +A P P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

[Close]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match: O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 4.5e-120
Identity = 218/466 (46.78%), Postives = 291/466 (62.45%), Query Frame = 0 

Query: 76  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 135
           F +TTP+YYVN  PH+G AYTTIAAD IAR+ RL    V F+TGTDEHG KI   A   G
Sbjct: 7   FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66

Query: 136 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 195
            +P E  D  +E +K LW+ L + Y KFIRTTDP H   V++ + +    GDIY  +YEG
Sbjct: 67  ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126

Query: 196 LYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQGLLEETLTQNPGFVQP 255
            YCV CEE+K E EL  ++ CPIH K C   KE +YFF LSKYQ  L E   +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186

Query: 256 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQ 315
            +R NE+ S+++ GLKD S++  R+ V+WGIPVP D + TIYVWFDAL  YISAL +   
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246

Query: 316 QQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 375
                  V   WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM 
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306

Query: 376 KSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 435
           K+LGN ++P E+VQ++G D VRYF LREV FG DGD+++   +N +N  LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366

Query: 436 TLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLSSACEAVLEIGNAG 495
            + +  K      +    + A    +    +E +    N+ E ++   A E +L+  +  
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426

Query: 496 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTP 540
           N Y+DE+ PW+L K+     +   K L  +++ + ++   L P+TP
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITP 456

[Close]
BLAST of MS003041 vs. ExPASy TrEMBL
Match: A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 610/616 (99.03%), Postives = 611/616 (99.19%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSS--LNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARINSSIQSSLWLLNPLQSS  LNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELV KARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVT 540
           LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAV LSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540

Query: 541 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           PCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 615
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616

[Close]
BLAST of MS003041 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 583/614 (94.95%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SLWLLNP  SSLNL    + RTRL+F +NLL  PS+  SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLK-PTHFRTRLHFCQNLLSFPSH--SSSKRAIFCTS 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
            SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFITGT
Sbjct: 61  TSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           KVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ 
Sbjct: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NLQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV KARN+YE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PKKP
Sbjct: 541 LSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQ VAE+
Sbjct: 601 VKSKKKIAQAVAES 611

[Close]
BLAST of MS003041 vs. ExPASy TrEMBL
Match: A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 560/615 (91.06%), Postives = 582/615 (94.63%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIFCT 60
           MAARIN+SIQ+SLWLLNPLQSS NL    +LRTR NFY+NLL +P S S+SSSKRAIFC+
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKAT-HLRTRFNFYQNLLSAPSSCSSSSSKRAIFCS 60

Query: 61  YASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120
            ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG
Sbjct: 61  SASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120

Query: 121 TDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 180
           TDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKEFY
Sbjct: 121 TDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFY 180

Query: 181 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQ 240
           S VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ
Sbjct: 181 SNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQ 240

Query: 241 GLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300
            LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVWFD
Sbjct: 241 RLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300

Query: 301 ALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360
           ALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV
Sbjct: 301 ALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360

Query: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 420
           FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFINIV
Sbjct: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIV 420

Query: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSL 480
           NAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELVAKARNHYE LSL
Sbjct: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEELSL 480

Query: 481 SSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTP 540
           SSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ LSPVTP
Sbjct: 481 SSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTP 540

Query: 541 CLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKK 600
            LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PKK
Sbjct: 541 RLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKK 600

Query: 601 PVKSKKKIAQGVAEA 615
           PVKSKKKIAQ VAEA
Sbjct: 601 PVKSKKKIAQKVAEA 614

[Close]
BLAST of MS003041 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 583/614 (94.95%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SL LLNP  +SLNL    + RTRL+FY+NLL   SY  SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK-PTHFRTRLHFYQNLLSFASY--SSSKRAIFCTT 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
            SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI ITGT
Sbjct: 61  TSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           KVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ 
Sbjct: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV KARNHYE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPKKP
Sbjct: 541 LSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQGVAEA
Sbjct: 601 VKSKKKIAQGVAEA 611

[Close]
BLAST of MS003041 vs. ExPASy TrEMBL
Match: A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 558/614 (90.88%), Postives = 582/614 (94.79%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTY 60
           MAARIN+SIQ+SL LLNP  +SLNL    + RTRL+FY+NLL   SY  SSSKRAIFCT 
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK-PTHFRTRLHFYQNLLSFASY--SSSKRAIFCTT 60

Query: 61  ASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGT 120
            SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI ITGT
Sbjct: 61  TSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILITGT 120

Query: 121 DEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180
           DEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS
Sbjct: 121 DEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYS 180

Query: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQG 240
           KVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKYQ 
Sbjct: 181 KVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQR 240

Query: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDA 300
           LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYVWFDA
Sbjct: 241 LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYVWFDA 300

Query: 301 LLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360
           LLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF
Sbjct: 301 LLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVF 360

Query: 361 GHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420
           GHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN
Sbjct: 361 GHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVN 420

Query: 421 AHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSLS 480
           AHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV KARNHYE L+LS
Sbjct: 421 AHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELALS 480

Query: 481 SACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPC 540
           SACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ LSPVTP 
Sbjct: 481 SACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVTPR 540

Query: 541 LSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKP 600
           LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPKKP
Sbjct: 541 LSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPKKP 600

Query: 601 VKSKKKIAQGVAEA 615
           VKSKKKIAQGVAEA
Sbjct: 601 VKSKKKIAQGVAEA 611

[Close]
BLAST of MS003041 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 913.7 bits (2360), Expect = 8.0e-266
Identity = 462/622 (74.28%), Postives = 524/622 (84.24%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIFCT 60
           MAARIN+S+ ++L  L P  + LN       R    F + L   P YS  SS KRA++CT
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKPFSFRRNSFRFSKKL---PYYSQFSSGKRALYCT 60

Query: 61  YASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120
            +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFITG
Sbjct: 61  SSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFITG 120

Query: 121 TDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 180
           TDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKEFY
Sbjct: 121 TDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFY 180

Query: 181 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQ 240
           ++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSKYQ
Sbjct: 181 ARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKYQ 240

Query: 241 GLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300
             LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWFD
Sbjct: 241 KPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFD 300

Query: 301 ALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360
           ALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKMV
Sbjct: 301 ALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMV 360

Query: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 420
           FGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI IV
Sbjct: 361 FGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKIV 420

Query: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSL 480
           NAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LSL
Sbjct: 421 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSL 480

Query: 481 SSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTP 540
           SSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAV LSPV P
Sbjct: 481 SSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAP 540

Query: 541 CLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE---- 600
           CLS RIY+QLG+++DQF + TW++TKWGGLK GQVM   +PVFARIE +   +EDE    
Sbjct: 541 CLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKKPK 600

Query: 601 VPKKPVKSKKKIAQ---GVAEA 615
           V KK  K+K K+ +    VAEA
Sbjct: 601 VGKKTGKAKVKVVEQTPTVAEA 616

[Close]
BLAST of MS003041 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 805.8 bits (2080), Expect = 2.4e-233
Identity = 402/521 (77.16%), Postives = 449/521 (86.18%), Query Frame = 0 

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIFCT 60
           MAARIN+S+ ++L  L P  + LN       R    F + L   P YS  SS KRA++CT
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKPFSFRRNSFRFSKKL---PYYSQFSSGKRALYCT 60

Query: 61  YASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITG 120
            +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFITG
Sbjct: 61  SSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFITG 120

Query: 121 TDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFY 180
           TDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKEFY
Sbjct: 121 TDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFY 180

Query: 181 SKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQ 240
           ++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSKYQ
Sbjct: 181 ARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKYQ 240

Query: 241 GLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFD 300
             LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWFD
Sbjct: 241 KPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWFD 300

Query: 301 ALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV 360
           ALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKMV
Sbjct: 301 ALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKMV 360

Query: 361 FGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIV 420
           FGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI IV
Sbjct: 361 FGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKIV 420

Query: 421 NAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVAKARNHYEGLSL 480
           NAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LSL
Sbjct: 421 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSL 480

Query: 481 SSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDL 521
           SSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAK L
Sbjct: 481 SSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515

[Close]
BLAST of MS003041 vs. TAIR 10
Match: AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )

HSP 1 Score: 164.9 bits (416), Expect = 2.1e-40
Identity = 145/571 (25.39%), Postives = 240/571 (42.03%), Query Frame = 0 

Query: 77  VITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 136
           +IT+ L YVN  PH+G+     ++AD  AR+ RL G   I+I GTDE+G    T A  + 
Sbjct: 20  LITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATETKALEEN 79

Query: 137 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 196
             P E CD     +K ++    +++DKF RT+ P+   + +  ++K+  N  +     + 
Sbjct: 80  CTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLSENTMQQ 139

Query: 197 LYCVNCEEYKDEKELLANNCCPIH----------------------------LKPCAS-- 256
           LYC  C+++  ++  L    CP                               K C +  
Sbjct: 140 LYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCKVCQNTP 199

Query: 257 --RKEDNYFFALSKYQGLLEETLTQNPGFVQPAFRLNEVQ---SWIRSGLKDFSISRASV 316
             R  D+ F  L   +  LE  + +    V  ++  N +Q   +W+R GL+   I+R  +
Sbjct: 200 RIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRCITR-DL 259

Query: 317 EWGIPVPND--QKQTIYVWFDALLGYISALSENGQQQNLQRAVASGW------PASLHL- 376
           +WG+PVP++  + +  YVWFDA +GY+S  S             S W      P ++ L 
Sbjct: 260 KWGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNPENVELY 319

Query: 377 --IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQ 436
             +GKD + FH V +P+  +       L K +    +L  +  K  KS G  +  N++  
Sbjct: 320 QFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKD 379

Query: 437 -KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK--NCQS 496
                +  RY+ L      SD  ++       +N  L + +GN +NR L  + K  N   
Sbjct: 380 TNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGY 439

Query: 497 TLVVDSTAAAEGN----SFKDKVEELVAKARNHYEGLSLSSACEAVLEIGNAGNSYMDEQ 556
             V+     AE +    S  +KVE+ VA+     E + L    +  + I + GN Y+   
Sbjct: 440 GSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQAS 499

Query: 557 APWSLFKQGGASSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLG----FS-KDQ 587
             W L+K+        A  +      + ++A  L P  P  S  ++ QL     FS  D+
Sbjct: 500 QFWKLYKE---DKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLPPQFSLSDE 559

[Close]
BLAST of MS003041 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 48.5 bits (114), Expect = 2.2e-05
Identity = 68/285 (23.86%), Postives = 116/285 (40.70%), Query Frame = 0 

Query: 297 WFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 356
           WF + L   S L   G      +   + +P ++   G DIL F    W A ++  G++  
Sbjct: 525 WFSSSLWPFSTL---GWPDVAAKDFNNFYPTNMLETGHDILFF----WVARMVMMGIEFT 584

Query: 357 KMV-FGHGFL-----TKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDY 416
             V F H +L        G KM KSLGN ++P + ++ FG+DA+R F +     G D + 
Sbjct: 585 GTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKDFGTDALR-FTIALGTAGQDLNL 644

Query: 417 AEDRFINIVNAHLANTI---GNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELV 476
           + +R     N    N +   G  +  +L  L        ++D     E       + E  
Sbjct: 645 STERL--TANKAFTNKLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECW 704

Query: 477 AKARNHYEGLSLSSACEAVLEIGNAGNSYMD----EQAPW-------SLFKQGGASSDAA 536
           A ++ H    S++++ E  L  G+ G    D    + A W        L+  GG S   A
Sbjct: 705 AVSKLHILIDSVTASYEK-LFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLA 764

Query: 537 AKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLGFSKDQFEAATW 562
           ++   ++L     I   L P  P ++  ++  L + K+    + W
Sbjct: 765 SQ--AVLLYVFENILKLLHPFMPFVTEDLWQALPYRKEALIVSPW 796

[Close]
BLAST of MS003041 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 47.4 bits (111), Expect = 4.9e-05
Identity = 77/313 (24.60%), Postives = 126/313 (40.26%), Query Frame = 0 

Query: 293 TIYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 352
           T+ VWFD+   +   L   G+++ L       +PA ++L G D    H  ++ + L+++ 
Sbjct: 652 TMDVWFDSGSSWAGVL---GKREGL------SFPADVYLEGTD---QHRGWFQSSLLTSI 711

Query: 353 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ---------KFGSDAVRYFF 412
              G      V  HGF L + GMKM KSLGN ++P  +++          +G+D +R + 
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771

Query: 413 LREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNS 472
                  S  DY  D  I        + I   L  TL  L  N      VD+    +   
Sbjct: 772 -------SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWR-VDNAVPYQDLP 831

Query: 473 FKD-----KVEELVAKARNHYEGLSLSSACEAV--LEIGNAGNSYMDEQAPWSLFKQGGA 532
             D     ++E +V   +  YE        + +    I +  N Y D  A   L+  G +
Sbjct: 832 IIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTS 891

Query: 533 SSDAAAKDLVLILEAMRIIAVTLSPVTPCLSWRIYAQLGF---SKDQFEAATWNETKWGG 582
           S    +   VL    + I+ V ++P+ P L+  ++  L F   ++D   A    E KW  
Sbjct: 892 SFTRRSCQTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPT 942

The following BLAST results are available for this feature:
BLAST of MS003041 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022133974.10.0e+0099.03methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_038891037.10.0e+0090.88methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_004142742.10.0e+0090.88methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... [more]
XP_022960433.10.0e+0091.06methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata][more]
KAA0066020.10.0e+0090.88methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ... [more]
BLAST of MS003041 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M2T91.1e-26474.28Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q557294.9e-15953.17Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Q8Z0684.1e-15853.35Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P590815.4e-15050.97Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... [more]
O672984.5e-12046.78Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ... [more]
BLAST of MS003041 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BXG80.0e+0099.03Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1[more]
A0A0A0KQV70.0e+0090.88Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1[more]
A0A6J1H8X30.0e+0091.06Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1[more]
A0A5D3C1K00.0e+0090.88Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CJS70.0e+0090.88Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1[more]
BLAST of MS003041 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G55400.18.0e-26674.28methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT3G55400.22.4e-23377.16methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT4G13780.12.1e-4025.39methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... [more]
AT5G16715.12.2e-0523.86ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
AT5G49030.14.9e-0524.60tRNA synthetase class I (I, L, M and V) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033911Methioninyl-tRNA synthetase core domainPRINTSPR01041TRNASYNTHMETcoord: 158..169
score: 51.54
coord: 110..124
score: 54.81
coord: 330..345
score: 65.28
coord: 422..433
score: 51.85
coord: 78..91
score: 56.61
coord: 294..305
score: 72.53
IPR033911Methioninyl-tRNA synthetase core domainCDDcd00814MetRS_corecoord: 75..410
e-value: 2.40741E-164
score: 470.09
NoneNo IPR availableGENE3D2.170.220.10coord: 187..296
e-value: 3.1E-140
score: 469.0
NoneNo IPR availableGENE3D1.10.730.10coord: 411..590
e-value: 1.0E-42
score: 147.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 588..614
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 587..614
NoneNo IPR availablePANTHERPTHR43326METHIONYL-TRNA SYNTHETASEcoord: 1..607
NoneNo IPR availablePANTHERPTHR43326:SF6BNAA09G34980D PROTEINcoord: 1..607
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 74..420
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 76..407
e-value: 3.1E-140
score: 469.0
IPR014758Methionyl-tRNA synthetaseTIGRFAMTIGR00398TIGR00398coord: 77..585
e-value: 2.2E-156
score: 519.6
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 69..201
e-value: 2.0E-8
score: 33.0
IPR015413Methionyl/Leucyl tRNA synthetasePFAMPF09334tRNA-synt_1gcoord: 210..435
e-value: 2.2E-62
score: 211.0
IPR023457Methionine-tRNA synthetase, type 2HAMAPMF_01228Met_tRNA_synth_type2coord: 67..581
score: 42.702507
IPR041872Methionyl-tRNA synthetase, anticodon-binding domainCDDcd07957Anticodon_Ia_Metcoord: 419..549
e-value: 4.91557E-36
score: 129.532
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 423..585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003041.1MS003041.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006431 methionyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004825 methionine-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding