MS003016 (gene) Bitter gourd (TR) v1

Overview
NameMS003016
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDUF2301 domain-containing protein
Locationscaffold595_1: 1046410 .. 1051763 (-)
RNA-Seq ExpressionMS003016
SyntenyMS003016
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGGAATTGGATCTTTATGTGCCCCCATTCTTTCTCTTTCGAAGCTTCGTTCTGTTAATTATTCAGCTGATAGCAAAACTAAGTTTCTGGTCGGTTCCGCAGTTGCTTTCCCTCCGCCATCTAAGAAGTTATCAGCTTCAGCTACTGGGCTAACCGTTCGAATTGGAACTGTCAGTGTCAAGTGCAAGGCTGCGGGCCAAACCCCGCCCAACACGGTATATCAGGGAATTTACGGTCCTTGGACCGTTGATCCTTCCGACGTTCGAGAGGTTCTACTTCTACTTGCTTTCTCTTTCCTTTTCTTTTTAATTGTTTACTGTTGTTATCTCTTATAATTTGGGATTTGCGGTTGCCGTGGAAGTTGCGGCTGATTCTATGTATTAACTCCGAAATCGTGTGAATTGTATCATATTATTTTTTATCTTGGAATTTATGTCATTAACTCCGTTTAAATATGGCCTGAATGATACGAGGATCCATGAGTTCATGTTGGAGTACTATAAAAAAGTAACATAGCCGCTTCTTTCAGCAATCAGCATCCTATCGAAAAAAATATCTTTCGTCTTCTTCTACGCCCTTCATTCACCGTTTCTAATCTTTTGGTTGCAGGTCTTTAGTCCTAAACATAAGTAGCGCACTATCACCAAAAACATTTCCAGGCTTGTATGAAATATGAAAACGTGTACAGACTAATTCAACCTTGATATTTTTAGTTCTTATTATTGTATAGAAAAACTACACAATTTGAACAGTAGCTCGAGAGAAAAAGAAAGATAGAGGAAATACGTGGAAGTATACAATTTCGTTCAATAATTGTTCCAAATAAATATACATTCTAATGCTAAAAATAGACTCAACAATCTCCACAATCAGCAACTTACTCCACTAACTCCACAAGGACAACTTAATTAACTTGACTACAACTAAAAATAACACCACCAACTTCCTAAAAACTAGGACTTGGACTAGACCAATTCAAAACTAGTGATCTCGAGTTAACTAACAATATTACTTGTCAAGTAGGTGATTTTATGTTCATATTTTCTTGATTGCATAAACATTTTTTATATTTGTGAGTGTTCTGGCCAGTTTGTGCGCATCTTGACTAATCTCATGGGACGACTGGATGACCCTATTACATTTTGTTGTCAAGCAAACTCATAGGGTACTAAATCCTAGGTAGGTGGTCACCATAGTTTAATCCCACTCTCTAAATTCTTTAGTATTTACCTTGGACCACTAGGCCAACTCATGAGAGTGTGTTGTCTAGAATTTTCTAGTAGCTCTTAGTTCATATTTTTGTAGCGTACCTAATGTCGTATCATCCAAGCCTCTTTACAGACAAGATCATGGTGCCTAGCTCAATAATTGAATATGATTTGTCGAAGTGCTTAGTTGTCCTTTACTGGGCTTTGTTTGATGACCATTTCGTTTTCAACTTTTTGTTTTTAAAAATTATACTTGTTTTCTCAGAATTTCTTTGGTAGGTTGTTCACGTTTTGGAAAAACACATTTGAATTCTTAGAAAAATTTAAAAAATAAAACTATTTTTTTTAGTATTCAAATCTTGGCTTAGATTTTGAGAACGTTCTTAGAAAATAGGTAACAAAATAAAGAAAAATGTGGAAGTAGTGTTTATTGAAAACAGAAAATAGAAAACAAAATGGTTATCAAATGACCCTAATAAATTGGAATTTGATGTGTATTCAATACTCATTGGAGAAGACAGTCTCATGTAGTTGAACCTGCGTAGACAGTCCCAAATATTTCAGTGATAATTTGCTTACTGTGGCAGGTGATTTTGTATAGAGCGGGCCTAGTGACAGCTGCTACCTCTTTTGTGATAGCTTCATCAGTTGCTTTTTTACCCGATACCTCTTCATTGAGTGACACACTTAAGCAAAATCTTGATCTGTTATATGCCTTAGGTGGAGGAGGATTAGGCTTATCCCTAGTTTTGATTCACATATATGTTACGGCAATTAAGCGTACTCTTCAAGCTTTATGGGTGCTTGGTGTTGCTGGATCTTTGGTAACTTACATATATCTTGCACAACCAGCTGGGGATGGCTTAGTGCAGTATGTTGTTGATAATCCATTGGCAGTCTGGTTTATTGGTCCTCTATATGCAGCACTGACTGGACTTGTCTTCAAAGAAGGTATCAAGTATTTCTTAATATCTTACAGGCTTTAAACTTAGTATTTGAGTCTTTAATATTGTATCAATGAAGCAAGCAAATAATAAGGAAGAAGATATTGAGGGTTAGACTTACTTTGCTTGTTCAGCATGTAGAAATTAGATTATTAATTATTAATTACATTGCAGAGGTTGTTATGATATTGGATTATTTAGTTTCAGGGTTCTGTCTCTTCATGGCCTGGCTTTTTCCCCCTTCTAGTCATTTTTTAAAATATAAAAACAACCTGTGGTGTATGGCATCTGAAATGCAGTGAGACATATATTTCAATCTGTAATATCAACTGGTTATGCATACTTTACGATTAGTGTCACTGTCAGTTTTACTTTTCAACACTTTCTTATCCTCTATACGTAGGGCTTTGCTATGGAAAGCTTGAGGCTGGAGTTCTCACCTTTGTCATACCTATGCTGCTTCTAGGGCATCTGGTAAGGTTCAACATTCACTGTTTTCCTATAAGCTAGACTTCTCTAATAAGGAGTTTGGCATAATATGACATGTATGGATAAGTTTTCAAACCTGGGTACCTCAACGCCTTGTACTGTTTGTGTGCATTATTTTGATCTGAAGGAATGTGCAAGGTTTTCACAAACCTGTGACGGCATAATGGACCAAATTATTAGTTTGAAACGGCAGTTTCTTTCTTCTAGTGTTAAAAGTTACTCTAAAAAATGCCCTTCTATGAATATGGAGAGCTGTATTTGTAAATTCTTGTCTCCTGATAAATTTCTATTAAACCTAAACTAAAAAGGATGCTGGAGCCTTTGAACTATCTGCAGTAGAGAATTTTTATTATGCTAATTTAGATTTTTCAATTTGTTATGCCAACATCTAAATAAGAAAAACATTCACTATCACACTTTTTATTTTTGTATGTTAAATGACTGTTACACGCTCAAAAGCTTAGACTAGATTTAAGCATATTGTTACACGCTCAAAAGCTTAGACTAGATTTAAGCATATATCACAATTGTCCCTCACATATGTAGCCCGCATATTTGTTTTACTTATGAGGCTCAACATGTGAATAACTTAAGGTAAAAGTAGGGAGAAATTAATTTTATAGAAATATAAATATATCATCTCCTATTTTTAATAATATATTAAATTACCATTAAAATCAAAATCTCAAACTGTCGAGTTTTAGATTTATTTACTTTTTTATTCTATTTTGATAGGAATGCGTTTAGTAATTAGGTATTAAGGATATATTAGTAATTGTGTTAGGAGGTTTGATTATAAATGGAGTATAGAGAAAGGATGAAGGTAGGCAATTCGTGAGTATGGTTTAGGCTTGAGTAAGAATACTCAAGAGGGGGAAGGTCCAGTCCAATTACCTTGAATCACTGGGTAGTTACTGTAGTTCTTATACTTTTTATATTTCAATATATTGTTAGTTGTTTGGGTTCTATCACATTTCTGGAATTTTTCTTTCATTATTGAAGGAAATTAATAGTGTATTACCAAACTGTTGAGATTTGATCTTATACTGGGTTATAATACAAGTTGGATTCTGTTAGGCCTCCAATCCTAAAAGTTAATTCTAGGAGGGGGAAATATGGGGAAATACTCTTCTGACAGTTAGTTGAATTTGGAATAGTATTTTGTGGGGGATGTCGGGCTTAGGATAGCAGGGCAGTTATCATTCTGGGTAAGGTACTAGAGTTTTGGAGAGCCACCAGCCCTCCCGAATTGGCTGGATAGCATGTAGTAGGGCGGTTTTCGAAAGTATAGTGCCTAATTCACTAACTCGTAGGGAGATATTGAACTTTTGTATTTGAAAAAAGTTTCAGGGGTGATGATCTCTAAAGTAGATGATGGTTGATAGAGTAATCCTTAATCATAATTTCCAGAATGAGTACTTGGGCCAACATGAAATTTGTGGTTGGTAGTAAAACACCAATTCTCCTCTTGAGACTTAATTCGATCTTCATCTCCTTACATAAATAAGACTCTAGATACCTAACAGATTACAAAATTTATTCTTTATATTACCATTGTATGTGGAAATCTATAATTTTGTATCTTAGTTAGACTAATGCTTTACGAAAGCTAGCTGAAGTAAAAAATCTTTCCGGAATTCAAATTTTAAGGATTTTTTAGAGGGTGACACTCGCAATGTGTTCAATTATTATTATTATTTGAGCCATAGACCTAAGATCTCTTTCTAGCGCTTGACCCTGTACACAGTACACTGACCTTTTGACCGACTTTACTTGAAATGTGCAGACTGGTTTGATGGACGATGGAGCTAAACTTGCTCTATTAGGTTCATGGATGGCCCTTTTTGTGATATTTGCTGGAAGAAAGTTCATTCAACCCATCAAGGTAATAAGCTCATGAACATTCTTTGAATTACTAAAGACTCCTCCATTCGATAACCATTTGGTTTAAAAAAATTAAGCTTACAAACACTATTTCTACTTTCTTTTCGGTAAACACTTTTATCTGTCTATTTGTTTAGTTATCTATCTTTAAAAAAAAGATCACAAAATCTAAGCTAAAATTTGAAAACTAAAAAAAAAAAGGTTTTAAAAACTTGGTTCCATTAGCACGGGCACGGTGCCGTGGGGCGGCAAGTGTGGGTGTCCCAGTTATAGGGGTAAAAAGTTAAAGAACCATTGATTCTAGGGTTATCAAAAAAAAAAAAGGGGACACCCTTGGTTCTGTTTGTAAAATTTGTCTTTGAATTAAATTTTTTAAGAAAACAAAGAACCTGAGGCCTTAGTGTTGGGTTCCTTTAGGAATGAAGGTTTAATACCATTTTTTCTGATTTTTAAAAACAAAAGTTACTTTTAGATTAAAAACTCATTTGTAATGATTTTTTTTAAAACTAGTTTTAAAAGGATAAAATGGAGAAAAACATTTTGTCGTTTATTGTACAGTATAGTATAGCATACAGAAACCTAATCACATTAGAAAGGGTAGAAAGCTGCAATGGAGTTATTTTGAAAACCAATTTTGGAAATACAGATACTAACCAAAACCATCAGTATGTAGAACATTTCATTGTTGCACTGTGTAACTGGTTTCTTATTTGTTTTCAGGATGATATCGGCGACAAATCTGTTTTCATGTTCAATGCACTCGGAGAGGATGAAAAGAAGGCCTTGATTGCAAAGCTTGAGCTGCAAGAGCTTAGTCAGAGTGCTGAT

mRNA sequence

TCTGGAATTGGATCTTTATGTGCCCCCATTCTTTCTCTTTCGAAGCTTCGTTCTGTTAATTATTCAGCTGATAGCAAAACTAAGTTTCTGGTCGGTTCCGCAGTTGCTTTCCCTCCGCCATCTAAGAAGTTATCAGCTTCAGCTACTGGGCTAACCGTTCGAATTGGAACTGTCAGTGTCAAGTGCAAGGCTGCGGGCCAAACCCCGCCCAACACGGTATATCAGGGAATTTACGGTCCTTGGACCGTTGATCCTTCCGACGTTCGAGAGGTGATTTTGTATAGAGCGGGCCTAGTGACAGCTGCTACCTCTTTTGTGATAGCTTCATCAGTTGCTTTTTTACCCGATACCTCTTCATTGAGTGACACACTTAAGCAAAATCTTGATCTGTTATATGCCTTAGGTGGAGGAGGATTAGGCTTATCCCTAGTTTTGATTCACATATATGTTACGGCAATTAAGCGTACTCTTCAAGCTTTATGGGTGCTTGGTGTTGCTGGATCTTTGGTAACTTACATATATCTTGCACAACCAGCTGGGGATGGCTTAGTGCAGTATGTTGTTGATAATCCATTGGCAGTCTGGTTTATTGGTCCTCTATATGCAGCACTGACTGGACTTGTCTTCAAAGAAGGGCTTTGCTATGGAAAGCTTGAGGCTGGAGTTCTCACCTTTGTCATACCTATGCTGCTTCTAGGGCATCTGACTGGTTTGATGGACGATGGAGCTAAACTTGCTCTATTAGGTTCATGGATGGCCCTTTTTGTGATATTTGCTGGAAGAAAGTTCATTCAACCCATCAAGGATGATATCGGCGACAAATCTGTTTTCATGTTCAATGCACTCGGAGAGGATGAAAAGAAGGCCTTGATTGCAAAGCTTGAGCTGCAAGAGCTTAGTCAGAGTGCTGAT

Coding sequence (CDS)

TCTGGAATTGGATCTTTATGTGCCCCCATTCTTTCTCTTTCGAAGCTTCGTTCTGTTAATTATTCAGCTGATAGCAAAACTAAGTTTCTGGTCGGTTCCGCAGTTGCTTTCCCTCCGCCATCTAAGAAGTTATCAGCTTCAGCTACTGGGCTAACCGTTCGAATTGGAACTGTCAGTGTCAAGTGCAAGGCTGCGGGCCAAACCCCGCCCAACACGGTATATCAGGGAATTTACGGTCCTTGGACCGTTGATCCTTCCGACGTTCGAGAGGTGATTTTGTATAGAGCGGGCCTAGTGACAGCTGCTACCTCTTTTGTGATAGCTTCATCAGTTGCTTTTTTACCCGATACCTCTTCATTGAGTGACACACTTAAGCAAAATCTTGATCTGTTATATGCCTTAGGTGGAGGAGGATTAGGCTTATCCCTAGTTTTGATTCACATATATGTTACGGCAATTAAGCGTACTCTTCAAGCTTTATGGGTGCTTGGTGTTGCTGGATCTTTGGTAACTTACATATATCTTGCACAACCAGCTGGGGATGGCTTAGTGCAGTATGTTGTTGATAATCCATTGGCAGTCTGGTTTATTGGTCCTCTATATGCAGCACTGACTGGACTTGTCTTCAAAGAAGGGCTTTGCTATGGAAAGCTTGAGGCTGGAGTTCTCACCTTTGTCATACCTATGCTGCTTCTAGGGCATCTGACTGGTTTGATGGACGATGGAGCTAAACTTGCTCTATTAGGTTCATGGATGGCCCTTTTTGTGATATTTGCTGGAAGAAAGTTCATTCAACCCATCAAGGATGATATCGGCGACAAATCTGTTTTCATGTTCAATGCACTCGGAGAGGATGAAAAGAAGGCCTTGATTGCAAAGCTTGAGCTGCAAGAGCTTAGTCAGAGTGCTGAT

Protein sequence

SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSDTLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQSAD
Homology
BLAST of MS003016 vs. NCBI nr
Match: XP_022148936.1 (uncharacterized protein LOC111017482 [Momordica charantia])

HSP 1 Score: 581.6 bits (1498), Expect = 3.8e-162
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV 60
           SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV
Sbjct: 3   SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV 62

Query: 61  KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL 120
           KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL
Sbjct: 63  KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL 122

Query: 121 SDTLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG 180
           SDTLKQNLDLLYA GGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG
Sbjct: 123 SDTLKQNLDLLYAFGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG 182

Query: 181 DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD 240
           DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD
Sbjct: 183 DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD 242

Query: 241 DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS 300
           DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS
Sbjct: 243 DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS 302

Query: 301 QSAD 305
           QSAD
Sbjct: 303 QSAD 306

BLAST of MS003016 vs. NCBI nr
Match: XP_004140559.1 (uncharacterized protein LOC101223108 [Cucumis sativus] >KGN46409.1 hypothetical protein Csa_005502 [Cucumis sativus])

HSP 1 Score: 465.3 bits (1196), Expect = 4.0e-127
Identity = 251/302 (83.11%), Postives = 264/302 (87.42%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS  KL S+NYSA +KTK L  S V+FPPPS KLSA              KCK
Sbjct: 4   GCLCASILSSPKLPSLNYSALTKTKLLRRSPVSFPPPS-KLSA-------------FKCK 63

Query: 64  AAGQTPPN-TVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQT P+ TVYQGIYGPWTVD SDVREVILYRAGLVTAATSFVIASSVAFLPD+SSL D
Sbjct: 64  AAGQTSPSPTVYQGIYGPWTVDSSDVREVILYRAGLVTAATSFVIASSVAFLPDSSSLGD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLY LGGGGLGLSL LIHIYVTAIKRTLQALWVLGVAGSLVTY+ L+QPAG+ 
Sbjct: 124 TLKQNLDLLYVLGGGGLGLSLFLIHIYVTAIKRTLQALWVLGVAGSLVTYLNLSQPAGES 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWF+GPLYAALTGLVFKEGLCYGKLEAG+LTFVIP LLLGHLTGLMDDG
Sbjct: 184 LVQYVVDNPSAVWFVGPLYAALTGLVFKEGLCYGKLEAGILTFVIPTLLLGHLTGLMDDG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
            KLALLGSWMALFVIFAGRKF QPIKDDIGDKSVF+FNALGEDEKKALIAKLE QE+SQ+
Sbjct: 244 VKLALLGSWMALFVIFAGRKFSQPIKDDIGDKSVFLFNALGEDEKKALIAKLEQQEVSQN 291

Query: 304 AD 305
           AD
Sbjct: 304 AD 291

BLAST of MS003016 vs. NCBI nr
Match: XP_022959722.1 (uncharacterized protein LOC111460706 [Cucurbita moschata])

HSP 1 Score: 464.9 bits (1195), Expect = 5.2e-127
Identity = 251/302 (83.11%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS S L S++YSAD KTK L+ S VAFP PS KLSA             +KCK
Sbjct: 4   GCLCASILSPSNLLSLDYSADIKTKLLLPSPVAFPSPS-KLSA-------------LKCK 63

Query: 64  AAGQ-TPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQ +P +TVY+GIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPD SSLSD
Sbjct: 64  AAGQSSPTSTVYRGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDNSSLSD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQA WVLGVAGSLV Y+ LAQPAGD 
Sbjct: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQAFWVLGVAGSLVAYVNLAQPAGDS 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWFIGPL+AALTGLVFKEGLCYGKLEAG+LTFVIP LLLGHL+GLMDDG
Sbjct: 184 LVQYVVDNPSAVWFIGPLFAALTGLVFKEGLCYGKLEAGILTFVIPTLLLGHLSGLMDDG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
           AKL LLGSWMALFVIFAGRKF QPIKDDIGDKSVFMFN LGEDEKKALIAKLE QEL Q+
Sbjct: 244 AKLGLLGSWMALFVIFAGRKFTQPIKDDIGDKSVFMFNDLGEDEKKALIAKLEQQELGQN 291

Query: 304 AD 305
            D
Sbjct: 304 VD 291

BLAST of MS003016 vs. NCBI nr
Match: XP_023004744.1 (uncharacterized protein LOC111497955 [Cucurbita maxima])

HSP 1 Score: 464.5 bits (1194), Expect = 6.8e-127
Identity = 252/302 (83.44%), Postives = 262/302 (86.75%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS S L S+NYSA  KTK L+ S VAFP PS KLSA             +KCK
Sbjct: 32  GCLCASILSPSNLLSLNYSAHIKTKLLLPSPVAFPSPS-KLSA-------------LKCK 91

Query: 64  AAGQ-TPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQ +P +TVY+GIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPD SSLSD
Sbjct: 92  AAGQSSPTSTVYRGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDNSSLSD 151

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQA WVLGVAGSLV Y+ LAQPAGD 
Sbjct: 152 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQAFWVLGVAGSLVAYVNLAQPAGDS 211

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWFIGPL+AALTGLVFKEGLCYGKLEAGVLTFVIP LLLGHL+GLMDDG
Sbjct: 212 LVQYVVDNPSAVWFIGPLFAALTGLVFKEGLCYGKLEAGVLTFVIPTLLLGHLSGLMDDG 271

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
           AKL LLGSWMALFVIFAGRKF QPIKDDIGDKSVFMFN LGEDEKKALIAKLE QEL Q+
Sbjct: 272 AKLGLLGSWMALFVIFAGRKFTQPIKDDIGDKSVFMFNDLGEDEKKALIAKLEQQELGQN 319

Query: 304 AD 305
            D
Sbjct: 332 VD 319

BLAST of MS003016 vs. NCBI nr
Match: KAG6592828.1 (40S ribosomal protein S27-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.1 bits (1185), Expect = 7.6e-126
Identity = 249/300 (83.00%), Postives = 262/300 (87.33%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS S LRS++YSAD KTK L+ S VAFP PS KLSA             +KCK
Sbjct: 4   GCLCASILSPSNLRSLDYSADIKTKLLLPSPVAFPSPS-KLSA-------------LKCK 63

Query: 64  AAGQ-TPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQ +P +TVY+GIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLP+ SSLSD
Sbjct: 64  AAGQSSPTSTVYRGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPENSSLSD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQA WVLGVAGSLV Y+ LAQPAGD 
Sbjct: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQAFWVLGVAGSLVAYVNLAQPAGDS 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWFIGPL+AALTGLVFKEGLCYGKLEAG+LTFVIP LLLGHL+GLMD+G
Sbjct: 184 LVQYVVDNPSAVWFIGPLFAALTGLVFKEGLCYGKLEAGILTFVIPTLLLGHLSGLMDNG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
           AKL LLGSWMALFVIFAGRKF QPIKDDIGDKSVFMFN LGEDEKKALIAKLE QEL  S
Sbjct: 244 AKLGLLGSWMALFVIFAGRKFTQPIKDDIGDKSVFMFNDLGEDEKKALIAKLEQQELDSS 289

BLAST of MS003016 vs. ExPASy TrEMBL
Match: A0A6J1D6V4 (uncharacterized protein LOC111017482 OS=Momordica charantia OX=3673 GN=LOC111017482 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV 60
           SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV
Sbjct: 3   SGIGSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSV 62

Query: 61  KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL 120
           KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL
Sbjct: 63  KCKAAGQTPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSL 122

Query: 121 SDTLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG 180
           SDTLKQNLDLLYA GGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG
Sbjct: 123 SDTLKQNLDLLYAFGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAG 182

Query: 181 DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD 240
           DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD
Sbjct: 183 DGLVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMD 242

Query: 241 DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS 300
           DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS
Sbjct: 243 DGAKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELS 302

Query: 301 QSAD 305
           QSAD
Sbjct: 303 QSAD 306

BLAST of MS003016 vs. ExPASy TrEMBL
Match: A0A0A0KC45 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G091330 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.9e-127
Identity = 251/302 (83.11%), Postives = 264/302 (87.42%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS  KL S+NYSA +KTK L  S V+FPPPS KLSA              KCK
Sbjct: 4   GCLCASILSSPKLPSLNYSALTKTKLLRRSPVSFPPPS-KLSA-------------FKCK 63

Query: 64  AAGQTPPN-TVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQT P+ TVYQGIYGPWTVD SDVREVILYRAGLVTAATSFVIASSVAFLPD+SSL D
Sbjct: 64  AAGQTSPSPTVYQGIYGPWTVDSSDVREVILYRAGLVTAATSFVIASSVAFLPDSSSLGD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLY LGGGGLGLSL LIHIYVTAIKRTLQALWVLGVAGSLVTY+ L+QPAG+ 
Sbjct: 124 TLKQNLDLLYVLGGGGLGLSLFLIHIYVTAIKRTLQALWVLGVAGSLVTYLNLSQPAGES 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWF+GPLYAALTGLVFKEGLCYGKLEAG+LTFVIP LLLGHLTGLMDDG
Sbjct: 184 LVQYVVDNPSAVWFVGPLYAALTGLVFKEGLCYGKLEAGILTFVIPTLLLGHLTGLMDDG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
            KLALLGSWMALFVIFAGRKF QPIKDDIGDKSVF+FNALGEDEKKALIAKLE QE+SQ+
Sbjct: 244 VKLALLGSWMALFVIFAGRKFSQPIKDDIGDKSVFLFNALGEDEKKALIAKLEQQEVSQN 291

Query: 304 AD 305
           AD
Sbjct: 304 AD 291

BLAST of MS003016 vs. ExPASy TrEMBL
Match: A0A6J1H5C2 (uncharacterized protein LOC111460706 OS=Cucurbita moschata OX=3662 GN=LOC111460706 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.5e-127
Identity = 251/302 (83.11%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS S L S++YSAD KTK L+ S VAFP PS KLSA             +KCK
Sbjct: 4   GCLCASILSPSNLLSLDYSADIKTKLLLPSPVAFPSPS-KLSA-------------LKCK 63

Query: 64  AAGQ-TPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQ +P +TVY+GIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPD SSLSD
Sbjct: 64  AAGQSSPTSTVYRGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDNSSLSD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQA WVLGVAGSLV Y+ LAQPAGD 
Sbjct: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQAFWVLGVAGSLVAYVNLAQPAGDS 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWFIGPL+AALTGLVFKEGLCYGKLEAG+LTFVIP LLLGHL+GLMDDG
Sbjct: 184 LVQYVVDNPSAVWFIGPLFAALTGLVFKEGLCYGKLEAGILTFVIPTLLLGHLSGLMDDG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
           AKL LLGSWMALFVIFAGRKF QPIKDDIGDKSVFMFN LGEDEKKALIAKLE QEL Q+
Sbjct: 244 AKLGLLGSWMALFVIFAGRKFTQPIKDDIGDKSVFMFNDLGEDEKKALIAKLEQQELGQN 291

Query: 304 AD 305
            D
Sbjct: 304 VD 291

BLAST of MS003016 vs. ExPASy TrEMBL
Match: A0A6J1KX72 (uncharacterized protein LOC111497955 OS=Cucurbita maxima OX=3661 GN=LOC111497955 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.3e-127
Identity = 252/302 (83.44%), Postives = 262/302 (86.75%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS S L S+NYSA  KTK L+ S VAFP PS KLSA             +KCK
Sbjct: 32  GCLCASILSPSNLLSLNYSAHIKTKLLLPSPVAFPSPS-KLSA-------------LKCK 91

Query: 64  AAGQ-TPPNTVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQ +P +TVY+GIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPD SSLSD
Sbjct: 92  AAGQSSPTSTVYRGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDNSSLSD 151

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQA WVLGVAGSLV Y+ LAQPAGD 
Sbjct: 152 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQAFWVLGVAGSLVAYVNLAQPAGDS 211

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWFIGPL+AALTGLVFKEGLCYGKLEAGVLTFVIP LLLGHL+GLMDDG
Sbjct: 212 LVQYVVDNPSAVWFIGPLFAALTGLVFKEGLCYGKLEAGVLTFVIPTLLLGHLSGLMDDG 271

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
           AKL LLGSWMALFVIFAGRKF QPIKDDIGDKSVFMFN LGEDEKKALIAKLE QEL Q+
Sbjct: 272 AKLGLLGSWMALFVIFAGRKFTQPIKDDIGDKSVFMFNDLGEDEKKALIAKLEQQELGQN 319

Query: 304 AD 305
            D
Sbjct: 332 VD 319

BLAST of MS003016 vs. ExPASy TrEMBL
Match: A0A5D3DMA3 (DUF2301 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001900 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 4.0e-125
Identity = 248/302 (82.12%), Postives = 261/302 (86.42%), Query Frame = 0

Query: 4   GSLCAPILSLSKLRSVNYSADSKTKFLVGSAVAFPPPSKKLSASATGLTVRIGTVSVKCK 63
           G LCA ILS  KL S+NYSA +KTK L  S V+FP PS KLSA             +KCK
Sbjct: 4   GCLCASILSYPKLPSLNYSALTKTKLLRRSPVSFPSPS-KLSA-------------LKCK 63

Query: 64  AAGQTPPN-TVYQGIYGPWTVDPSDVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSD 123
           AAGQT P+ TVYQGIYGPWTVD SDVREVILYRAGLVTAATSFVIASSVAFLPD SSL D
Sbjct: 64  AAGQTSPSPTVYQGIYGPWTVDSSDVREVILYRAGLVTAATSFVIASSVAFLPDNSSLGD 123

Query: 124 TLKQNLDLLYALGGGGLGLSLVLIHIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDG 183
           TLKQNLDLLY LGGGGLGLSL LIHIYVTAIKRTLQALWVLGVAGSLVTY  LAQPAG+ 
Sbjct: 124 TLKQNLDLLYVLGGGGLGLSLFLIHIYVTAIKRTLQALWVLGVAGSLVTYSNLAQPAGES 183

Query: 184 LVQYVVDNPLAVWFIGPLYAALTGLVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDG 243
           LVQYVVDNP AVWF+GPLYAALTGLVFKEGLCYGKLEAG+LTF+IP LLLGHLTGLMDDG
Sbjct: 184 LVQYVVDNPSAVWFVGPLYAALTGLVFKEGLCYGKLEAGILTFIIPTLLLGHLTGLMDDG 243

Query: 244 AKLALLGSWMALFVIFAGRKFIQPIKDDIGDKSVFMFNALGEDEKKALIAKLELQELSQS 303
            KLALLGSWMALFVIFAGRKF QPIKDDIGDKSVF+FNALGE+EKKALIAKLE Q +SQ+
Sbjct: 244 VKLALLGSWMALFVIFAGRKFSQPIKDDIGDKSVFIFNALGEEEKKALIAKLEQQGVSQN 291

Query: 304 AD 305
           AD
Sbjct: 304 AD 291

BLAST of MS003016 vs. TAIR 10
Match: AT1G28140.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2301, transmembrane (InterPro:IPR019275); Has 140 Blast hits to 140 proteins in 72 species: Archae - 0; Bacteria - 86; Metazoa - 10; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 338.2 bits (866), Expect = 6.9e-93
Identity = 174/273 (63.74%), Postives = 206/273 (75.46%), Query Frame = 0

Query: 33  SAVAFPPPSKKLSASATGLTVRIG------TVSVKCKAAGQTPPNTVYQGIYGPWTVDPS 92
           S      P    + S   LT R+G       V     ++      TVY+G+YGPWT+D +
Sbjct: 7   STTTLVTPPAYFNKSPAFLTARVGVRRGRANVKAVSNSSQGAVDGTVYKGVYGPWTIDQA 66

Query: 93  DVREVILYRAGLVTAATSFVIASSVAFLPDTSSLSDTLKQNLDLLYALGGGGLGLSLVLI 152
           DV+EVILYR+GLVTAA SFV ASS AFLP  S LS+T+KQN DL Y +G  GLGLSL LI
Sbjct: 67  DVKEVILYRSGLVTAAASFVAASSAAFLPGDSWLSETIKQNHDLFYFVGASGLGLSLFLI 126

Query: 153 HIYVTAIKRTLQALWVLGVAGSLVTYIYLAQPAGDGLVQYVVDNPLAVWFIGPLYAALTG 212
           HIYVT IKRTLQALW LG  GS  TY  LA+PAGD LV YVVD+P AVWF+GPL+A+LTG
Sbjct: 127 HIYVTEIKRTLQALWALGFVGSFATYAALARPAGDNLVHYVVDHPSAVWFVGPLFASLTG 186

Query: 213 LVFKEGLCYGKLEAGVLTFVIPMLLLGHLTGLMDDGAKLALLGSWMALFVIFAGRKFIQP 272
           LVFKEGLCYGKLEAG+LTF+IP +LLGHL+GLM+D  KL LLG+WMALF++FAGRKF QP
Sbjct: 187 LVFKEGLCYGKLEAGLLTFIIPSVLLGHLSGLMNDEVKLVLLGTWMALFLVFAGRKFTQP 246

Query: 273 IKDDIGDKSVFMFNALGEDEKKALIAKLELQEL 300
           IKDDIGDKSVF F +L +DEKKA++ KLE ++L
Sbjct: 247 IKDDIGDKSVFTFMSLSDDEKKAIVEKLEQEKL 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148936.13.8e-16299.67uncharacterized protein LOC111017482 [Momordica charantia][more]
XP_004140559.14.0e-12783.11uncharacterized protein LOC101223108 [Cucumis sativus] >KGN46409.1 hypothetical ... [more]
XP_022959722.15.2e-12783.11uncharacterized protein LOC111460706 [Cucurbita moschata][more]
XP_023004744.16.8e-12783.44uncharacterized protein LOC111497955 [Cucurbita maxima][more]
KAG6592828.17.6e-12683.0040S ribosomal protein S27-2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D6V41.9e-16299.67uncharacterized protein LOC111017482 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A0A0KC451.9e-12783.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G091330 PE=4 SV=1[more]
A0A6J1H5C22.5e-12783.11uncharacterized protein LOC111460706 OS=Cucurbita moschata OX=3662 GN=LOC1114607... [more]
A0A6J1KX723.3e-12783.44uncharacterized protein LOC111497955 OS=Cucurbita maxima OX=3661 GN=LOC111497955... [more]
A0A5D3DMA34.0e-12582.12DUF2301 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G28140.16.9e-9363.74unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019275Protein of unknown function DUF2301PFAMPF10063DUF2301coord: 141..279
e-value: 1.6E-53
score: 180.7
IPR019275Protein of unknown function DUF2301PANTHERPTHR36716F3H9.20 PROTEINcoord: 43..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003016.1MS003016.1mRNA