MS003006 (gene) Bitter gourd (TR) v1

Overview
NameMS003006
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReceptor-like protein kinase
Locationscaffold595_1: 954673 .. 956171 (-)
RNA-Seq ExpressionMS003006
SyntenyMS003006
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTCCTAATGCTCGCATATATCACTCTTCTCTTGCTCTTCATACTTTCGTTTCCTCTCTTTTCATGCGGAGGGTTTCAAACCCATGCCCTTGTTGCTTCCTCCGACCATCTCGTGCCATTTCCTGAAACTACTTCCCATTTCAAGAATGGGTTTCAAAGGATTCTCCTCAGCGTCGCATTAGGCGTCGTCACTGGATTCGCCGGCGCTTTTCTCTCTGCTTGGTTCATCCGCACCCTTGTGAGTTACCTAAACAGAACCCCACTTCTTGAAGGACCCCTAATATTTTCCCCCAAAATCGACCCCGACGCTCTCCAGTCAGCGCTGCGGAACGAGACCCAGTTGCTCGGTTCGAGTTCGAGCCCACACGCGAAATACTACAGGGCCATTCTCGCTAATGGCCTCGCATTGGCGATCAAGAAGCTTCAACCCATTCAATGCAGAAAGGCGGAGGCACGGCGGCGGATGCAGCACCAGCTGGCGGTTCTTGCCAGTTTGAGGCATCGGAATCTGATGACCTTACAGGCCTACGTCGGCGAAATGGGCAGCTGCTGCTTGGTTTATGACTTGGTGTCCAATGGTTCTCTCGAGGACGCAATGAGGAAAGTGAGAGAGAACCAATTGGAACTTAAGTGGGAGGTTCGGCTGAGAATTGCTGTTGGGATTGCGAGAGGGCTTCTGTTTCTCCACTTTGAATGCGACCCTCCAATTCTTCACCGCAATTTGAAGCCTTCGAATGTGCTGTTGGATGCAGAGTTCGAACCAAGGTTGGCTGATTGTGGGTTAATCAATCTCGTGCTCAATTTTGATAGGCCACCTTCTTTGTACACCGCTCCCGAGTGTCTTAACAGCTCCAGGTAAATCTCCAAAACCCCAAAGTTCCAAATAATAGAATAATGCTCTAGTCCCTGTCTCTGAAAATGCAAGAAATGCTTTTGGATATAGTTCGTTTAAGTTCATTTAGTTGGGTTCTTTTCTATTCGCATTACATGGCTCAGCTAGCTCTACTGATACTGATGAGATATTAAGTTCACAATGTTGATAAGATATTAAGTTTAGCTGAAGCTTAATAAACATCTTCAATGCCATCTTCCCTTTGTGCAAGAATGGTCGTATTCCTCTGATTTCTAGGTGAGAAACTAGGAGTGGGTTTTAGTTGCATACTTAATCAAAAGCTAGAAACGTATCCAATGGTGGTAATGGTGGTGTTGACCCTGAGCAGGTATACTGATAAGAGCGACATTTTCAGCTTTGGGGTGATACTGGGCATTCTGTTAAGCAGTAGAGATGTGATGGAGCAGTCGTCATCGGACGGGGAAGGCGAGGAGACTGGGGAAGCGGCGGTGGACAGAAGCATAAGCTTTAGAGAAGAAGGGGTGGGAGAAGATGAGATGGCGTTAGCATTGAGAATTGCTGGTGTGTGCGTATCAGATTTGGCTGAGGACAGACCTTCAAGCGATGAGTTACTTCTGATGCTGACCCAATTACATTGCTTT

mRNA sequence

ATGGGGTTCCTAATGCTCGCATATATCACTCTTCTCTTGCTCTTCATACTTTCGTTTCCTCTCTTTTCATGCGGAGGGTTTCAAACCCATGCCCTTGTTGCTTCCTCCGACCATCTCGTGCCATTTCCTGAAACTACTTCCCATTTCAAGAATGGGTTTCAAAGGATTCTCCTCAGCGTCGCATTAGGCGTCGTCACTGGATTCGCCGGCGCTTTTCTCTCTGCTTGGTTCATCCGCACCCTTGTGAGTTACCTAAACAGAACCCCACTTCTTGAAGGACCCCTAATATTTTCCCCCAAAATCGACCCCGACGCTCTCCAGTCAGCGCTGCGGAACGAGACCCAGTTGCTCGGTTCGAGTTCGAGCCCACACGCGAAATACTACAGGGCCATTCTCGCTAATGGCCTCGCATTGGCGATCAAGAAGCTTCAACCCATTCAATGCAGAAAGGCGGAGGCACGGCGGCGGATGCAGCACCAGCTGGCGGTTCTTGCCAGTTTGAGGCATCGGAATCTGATGACCTTACAGGCCTACGTCGGCGAAATGGGCAGCTGCTGCTTGGTTTATGACTTGGTGTCCAATGGTTCTCTCGAGGACGCAATGAGGAAAGTGAGAGAGAACCAATTGGAACTTAAGTGGGAGGTTCGGCTGAGAATTGCTGTTGGGATTGCGAGAGGGCTTCTGTTTCTCCACTTTGAATGCGACCCTCCAATTCTTCACCGCAATTTGAAGCCTTCGAATGTGCTGTTGGATGCAGAGTTCGAACCAAGGTTGGCTGATTGTGGGTTAATCAATCTCGTGCTCAATTTTGATAGGCCACCTTCTTTGTACACCGCTCCCGAGTGTCTTAACAGCTCCAGCAGGTATACTGATAAGAGCGACATTTTCAGCTTTGGGGTGATACTGGGCATTCTGTTAAGCAGTAGAGATGTGATGGAGCAGTCGTCATCGGACGGGGAAGGCGAGGAGACTGGGGAAGCGGCGGTGGACAGAAGCATAAGCTTTAGAGAAGAAGGGGTGGGAGAAGATGAGATGGCGTTAGCATTGAGAATTGCTGGTGTGTGCGTATCAGATTTGGCTGAGGACAGACCTTCAAGCGATGAGTTACTTCTGATGCTGACCCAATTACATTGCTTT

Coding sequence (CDS)

ATGGGGTTCCTAATGCTCGCATATATCACTCTTCTCTTGCTCTTCATACTTTCGTTTCCTCTCTTTTCATGCGGAGGGTTTCAAACCCATGCCCTTGTTGCTTCCTCCGACCATCTCGTGCCATTTCCTGAAACTACTTCCCATTTCAAGAATGGGTTTCAAAGGATTCTCCTCAGCGTCGCATTAGGCGTCGTCACTGGATTCGCCGGCGCTTTTCTCTCTGCTTGGTTCATCCGCACCCTTGTGAGTTACCTAAACAGAACCCCACTTCTTGAAGGACCCCTAATATTTTCCCCCAAAATCGACCCCGACGCTCTCCAGTCAGCGCTGCGGAACGAGACCCAGTTGCTCGGTTCGAGTTCGAGCCCACACGCGAAATACTACAGGGCCATTCTCGCTAATGGCCTCGCATTGGCGATCAAGAAGCTTCAACCCATTCAATGCAGAAAGGCGGAGGCACGGCGGCGGATGCAGCACCAGCTGGCGGTTCTTGCCAGTTTGAGGCATCGGAATCTGATGACCTTACAGGCCTACGTCGGCGAAATGGGCAGCTGCTGCTTGGTTTATGACTTGGTGTCCAATGGTTCTCTCGAGGACGCAATGAGGAAAGTGAGAGAGAACCAATTGGAACTTAAGTGGGAGGTTCGGCTGAGAATTGCTGTTGGGATTGCGAGAGGGCTTCTGTTTCTCCACTTTGAATGCGACCCTCCAATTCTTCACCGCAATTTGAAGCCTTCGAATGTGCTGTTGGATGCAGAGTTCGAACCAAGGTTGGCTGATTGTGGGTTAATCAATCTCGTGCTCAATTTTGATAGGCCACCTTCTTTGTACACCGCTCCCGAGTGTCTTAACAGCTCCAGCAGGTATACTGATAAGAGCGACATTTTCAGCTTTGGGGTGATACTGGGCATTCTGTTAAGCAGTAGAGATGTGATGGAGCAGTCGTCATCGGACGGGGAAGGCGAGGAGACTGGGGAAGCGGCGGTGGACAGAAGCATAAGCTTTAGAGAAGAAGGGGTGGGAGAAGATGAGATGGCGTTAGCATTGAGAATTGCTGGTGTGTGCGTATCAGATTTGGCTGAGGACAGACCTTCAAGCGATGAGTTACTTCTGATGCTGACCCAATTACATTGCTTT

Protein sequence

MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGVILGILLSSRDVMEQSSSDGEGEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF
Homology
BLAST of MS003006 vs. NCBI nr
Match: XP_022148904.1 (inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X1 [Momordica charantia])

HSP 1 Score: 725.3 bits (1871), Expect = 2.7e-205
Identity = 375/379 (98.94%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60
           MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV
Sbjct: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60

Query: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120
           ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS
Sbjct: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120

Query: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180
           SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG
Sbjct: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180

Query: 181 EMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240
           EMGSCCLVYDLV NGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH
Sbjct: 181 EMGSCCLVYDLVPNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240

Query: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGV 300
           RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLN SSRYTDKSDIFSFGV
Sbjct: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLN-SSRYTDKSDIFSFGV 300

Query: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLA 360
           ILGILLSSRDVMEQSSSDGEGEETGEAAVD SISFREEGVGEDEMALALRIAGVCVSDLA
Sbjct: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDGSISFREEGVGEDEMALALRIAGVCVSDLA 360

Query: 361 EDRPSSDELLLMLTQLHCF 380
           ED PSSDELLLMLTQLHCF
Sbjct: 361 EDXPSSDELLLMLTQLHCF 378

BLAST of MS003006 vs. NCBI nr
Match: XP_022148905.1 (inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X2 [Momordica charantia])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 366/379 (96.57%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60
           MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV
Sbjct: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60

Query: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120
           ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS
Sbjct: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120

Query: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180
           SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG
Sbjct: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180

Query: 181 EMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240
           EMGSCCLVYDLV NGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH
Sbjct: 181 EMGSCCLVYDLVPNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240

Query: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGV 300
           RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSS          SFGV
Sbjct: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSS----------SFGV 300

Query: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLA 360
           ILGILLSSRDVMEQSSSDGEGEETGEAAVD SISFREEGVGEDEMALALRIAGVCVSDLA
Sbjct: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDGSISFREEGVGEDEMALALRIAGVCVSDLA 360

Query: 361 EDRPSSDELLLMLTQLHCF 380
           ED PSSDELLLMLTQLHCF
Sbjct: 361 EDXPSSDELLLMLTQLHCF 369

BLAST of MS003006 vs. NCBI nr
Match: KAG7014619.1 (Inactive leucine-rich repeat receptor-like protein kinase CORYNE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 514.6 bits (1324), Expect = 7.2e-142
Identity = 283/401 (70.57%), Postives = 315/401 (78.55%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFS--CGGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           MGF      TLLLLF+  FP FS   GG QT ALV SS+H VP P+T  HFKNGFQRILL
Sbjct: 1   MGFFAFTIHTLLLLFVFWFPFFSGAKGGVQTDALVGSSEHFVPSPKTL-HFKNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGVVTG  GA LS WFIR+LV YLNRTPLLEGPLIFSPKID DALQSALRN+TQLLG
Sbjct: 61  SVALGVVTGLTGALLSVWFIRSLVGYLNRTPLLEGPLIFSPKIDADALQSALRNQTQLLG 120

Query: 121 --SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQ 180
             SSSSP+ K YR +LANGL +A+K+L+PI+C +AEARRRMQ QLAVL  L+HRN+M+L+
Sbjct: 121 SSSSSSPNGKCYREVLANGLTVAVKRLEPIECGRAEARRRMQQQLAVLGGLKHRNVMSLR 180

Query: 181 AYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDP 240
           AYV EMGSCCLVYD V +GSLEDAMRKVRENQLEL WEVRLRIAVGIARGL FLHFEC+P
Sbjct: 181 AYVAEMGSCCLVYDFVPSGSLEDAMRKVRENQLELNWEVRLRIAVGIARGLQFLHFECNP 240

Query: 241 PILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIF 300
            ILH NLKPSNVLLDAEFEPRLAD GLINL+ +FD PPSLYTAPECLN SSRYTDKSDIF
Sbjct: 241 RILHCNLKPSNVLLDAEFEPRLADFGLINLISDFDAPPSLYTAPECLN-SSRYTDKSDIF 300

Query: 301 SFGVILGILLSSRDVMEQSSSDGEGEETG---------------EAAVDRSI---SFREE 360
           SFGVIL +LL+ RD  EQ+S D  G+++G                 A+D++I      EE
Sbjct: 301 SFGVILSVLLTGRDPTEQASLD-RGDDSGSEGRLGRWLDQSVEPREALDKAIRGEGMEEE 360

Query: 361 GVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
              E+EM LALRIAGVC S+L EDRP SDELL MLTQL  F
Sbjct: 361 EEEEEEMVLALRIAGVCASELPEDRPCSDELLAMLTQLQSF 398

BLAST of MS003006 vs. NCBI nr
Match: KAG6576100.1 (Inactive leucine-rich repeat receptor-like protein kinase CORYNE, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.2 bits (1323), Expect = 9.4e-142
Identity = 283/400 (70.75%), Postives = 315/400 (78.75%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFS--CGGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           MGF      TLLLLF+  FP FS   GG QT ALV SS+H VP P+T  HFKNGFQRILL
Sbjct: 1   MGFFAFTIHTLLLLFVFWFPFFSGAKGGVQTDALVGSSEHFVPSPKTL-HFKNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGVVTG  GA LS WFIR+LV YLNRTPLLEGPLIFSPKID DALQSALRN+TQLLG
Sbjct: 61  SVALGVVTGLTGALLSVWFIRSLVGYLNRTPLLEGPLIFSPKIDADALQSALRNQTQLLG 120

Query: 121 --SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQ 180
             SSSSP+ K YR +LANGL +A+K+L+PI+C +AEARRRMQ QLAVL  L+HRN+M+L+
Sbjct: 121 SSSSSSPNGKCYREVLANGLTVAVKRLEPIECGRAEARRRMQQQLAVLGGLKHRNVMSLR 180

Query: 181 AYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDP 240
           AYV EMGSCCLVYD V +GSLEDAMRKVRENQLEL WEVRLRIAVGIARGL FLHFEC+P
Sbjct: 181 AYVAEMGSCCLVYDFVPSGSLEDAMRKVRENQLELNWEVRLRIAVGIARGLQFLHFECNP 240

Query: 241 PILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIF 300
            ILH NLKPSNVLLDAEFEPRLAD GLINL+ +FD PPSLYTAPECLN SSRYTDKSDIF
Sbjct: 241 RILHCNLKPSNVLLDAEFEPRLADFGLINLISDFDAPPSLYTAPECLN-SSRYTDKSDIF 300

Query: 301 SFGVILGILLSSRDVMEQSSSDGEGEETGEAA-----VDRSISFR------------EEG 360
           SFGVIL +LL+ RD  EQ+S D  G+++G        +D+S+  R            EE 
Sbjct: 301 SFGVILSVLLTGRDPTEQASLD-RGDDSGSEGRLGRWLDQSVEPREALDKAIRGEGMEEE 360

Query: 361 VGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
             E+EM LALRIAGVC S+L EDRP SDELL MLTQL  F
Sbjct: 361 EEEEEMVLALRIAGVCASELPEDRPCSDELLAMLTQLQSF 397

BLAST of MS003006 vs. NCBI nr
Match: XP_022953555.1 (inactive leucine-rich repeat receptor-like protein kinase CORYNE [Cucurbita moschata])

HSP 1 Score: 512.3 bits (1318), Expect = 3.6e-141
Identity = 282/401 (70.32%), Postives = 314/401 (78.30%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFS--CGGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           MGF      TLLLLF+  FP FS   GG QT ALV SS+H VP P+T  HFKNGFQRILL
Sbjct: 1   MGFFAFTIHTLLLLFVFWFPFFSGAKGGVQTDALVGSSEHFVPSPKTL-HFKNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGVVTG  GA LS WFIR+LV YLNRTPLLEGPLIFSPKID DALQSALRN+TQLLG
Sbjct: 61  SVALGVVTGLTGALLSVWFIRSLVGYLNRTPLLEGPLIFSPKIDADALQSALRNQTQLLG 120

Query: 121 ----SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMT 180
               SSSSP+ K YR +LANGL +A+K+L+PI+C +AEARRRMQ QLAVL  L+HRN+M+
Sbjct: 121 SSSSSSSSPNGKCYREVLANGLTVAVKRLEPIECGRAEARRRMQQQLAVLGGLKHRNVMS 180

Query: 181 LQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFEC 240
           L+AYV EMGSCCLVYD V +GSLEDAMRKVRENQLEL WEVRLRIAVGIARGL FLHFEC
Sbjct: 181 LRAYVAEMGSCCLVYDFVPSGSLEDAMRKVRENQLELNWEVRLRIAVGIARGLQFLHFEC 240

Query: 241 DPPILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSD 300
           +P ILH NLKPSNVLLDAEFEPRLAD GL NL+ +FD PPSLYTAPECLN SSRYTDKSD
Sbjct: 241 NPRILHGNLKPSNVLLDAEFEPRLADFGLTNLISDFDAPPSLYTAPECLN-SSRYTDKSD 300

Query: 301 IFSFGVILGILLSSRDVMEQSSSDGEGEETGEAA-----VDRSISFRE-----------E 360
           IFSFGVIL +LL+ RD  EQ+S D  G+++G        +D+S+  RE           E
Sbjct: 301 IFSFGVILSVLLTGRDPTEQASLD-RGDDSGSEGRLGRWLDQSVEPREALDKGIRGEGME 360

Query: 361 GVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
              E+EM LALRIAGVC S+L EDRP SDELL MLTQL  F
Sbjct: 361 EEEEEEMVLALRIAGVCASELPEDRPCSDELLAMLTQLQSF 398

BLAST of MS003006 vs. ExPASy Swiss-Prot
Match: Q9LYU7 (Inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Arabidopsis thaliana OX=3702 GN=CRN PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.4e-86
Identity = 187/393 (47.58%), Postives = 258/393 (65.65%), Query Frame = 0

Query: 9   ITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGF--QRILLSVALGVVT 68
           I+LLLLF L F  FS     T     +  HL     +++  ++    + I++S+  G++T
Sbjct: 16  ISLLLLFFLVF--FSRTSTSTSCRRRTVKHLSTTSTSSTPLESRITSKVIVISIVSGILT 75

Query: 69  GFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSSSSPHAK 128
           G   A + A+ +R++V ++ +TP+L+GP++FSPKI P +L +AL N  QLLGS    + K
Sbjct: 76  GLVSALVLAFLVRSIVKFMKQTPILKGPVVFSPKITPKSLHAALSNGIQLLGSDL--NGK 135

Query: 129 YYRAILANGLALAIKKL-------QPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYV 188
           YY+ +L NGL +A+K+L        P        +RR+Q +L +LA LRHRNLM+L+AYV
Sbjct: 136 YYKMVLDNGLVVAVKRLGSLEGVGSPESSSSKSVKRRLQKELELLAGLRHRNLMSLRAYV 195

Query: 189 GEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPIL 248
            E     LVYD + NGSLED M KVR  ++EL WE+RLR+AVGI +GL +LHF C+  IL
Sbjct: 196 RESDEFSLVYDYMPNGSLEDVMNKVRTKEVELGWEIRLRVAVGIVKGLQYLHFSCETQIL 255

Query: 249 HRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFG 308
           H NLKP+NV+LD+EFEPRLADCGL  ++ +     S Y+APE  + S+RYTDKSDIFSFG
Sbjct: 256 HYNLKPTNVMLDSEFEPRLADCGLAKIMPSSHTAVSCYSAPES-SQSNRYTDKSDIFSFG 315

Query: 309 VILGILLSSRD----VMEQSSSDG-------EGEETGEA--AVDRSISFREEGVGEDEMA 368
           +ILG+LL+ RD      E+S+S G         +++GEA  A+D++I   E  V EDEM 
Sbjct: 316 MILGVLLTGRDPTHPFCEESASGGSLGQWLKHLQQSGEAREALDKTILGEE--VEEDEML 375

Query: 369 LALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
           +ALRI  +C+SD   DRPSSDEL+ MLTQLH F
Sbjct: 376 MALRITIICLSDFPADRPSSDELVHMLTQLHSF 401

BLAST of MS003006 vs. ExPASy Swiss-Prot
Match: Q9SKB2 (Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 OS=Arabidopsis thaliana OX=3702 GN=SOBIR1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.4e-31
Identity = 108/358 (30.17%), Postives = 181/358 (50.56%), Query Frame = 0

Query: 57  LLSVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNE--- 116
           +L   +G + G    F+ +   + ++  +  +    GP IFSP I      + L NE   
Sbjct: 288 ILGFVVGAIGGTISGFVFSVLFKLIIQAIRGSEKPPGPSIFSPLIKKAEDLAFLENEEAL 347

Query: 117 --TQLLGSSSSPHAKYYRAIL--ANGLALAIKK-LQPIQCRKA----------EARRRMQ 176
              +++G       + ++A L  +NG  +A+KK +QP +              +  R+++
Sbjct: 348 ASLEIIGRGGC--GEVFKAELPGSNGKIIAVKKVIQPPKDADELTDEDSKFLNKKMRQIR 407

Query: 177 HQLAVLASLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLR 236
            ++  +  +RHRNL+ L A+V       LVY+ +  GSL+D +  V+    EL W  R +
Sbjct: 408 SEINTVGHIRHRNLLPLLAHVSRPECHYLVYEYMEKGSLQDILTDVQAGNQELMWPARHK 467

Query: 237 IAVGIARGLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGL--------INLVLNF 296
           IA+GIA GL +LH + +P I+HR+LKP+NVLLD + E R++D GL         ++  + 
Sbjct: 468 IALGIAAGLEYLHMDHNPRIIHRDLKPANVLLDDDMEARISDFGLAKAMPDAVTHITTSH 527

Query: 297 DRPPSLYTAPECLNSSSRYTDKSDIFSFGVILGILLSSR---DVMEQSSSDGE------- 356
                 Y APE    + ++TDK DI+SFGVILGIL+  +   D   Q + +         
Sbjct: 528 VAGTVGYIAPE-FYQTHKFTDKCDIYSFGVILGILVIGKLPSDEFFQHTDEMSLIKWMRN 587

Query: 357 --GEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQL 377
               E    A+D  +   ++G  +++M L L+IA  C  D  + RP+S ++  ML+Q+
Sbjct: 588 IITSENPSLAIDPKL--MDQGF-DEQMLLVLKIACYCTLDDPKQRPNSKDVRTMLSQI 639

BLAST of MS003006 vs. ExPASy Swiss-Prot
Match: Q9FII5 (Leucine-rich repeat receptor-like protein kinase TDR OS=Arabidopsis thaliana OX=3702 GN=TDR PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 9.1e-31
Identity = 98/293 (33.45%), Postives = 153/293 (52.22%), Query Frame = 0

Query: 104 DALQSALRNETQLLGSSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLA- 163
           D +   L     +LG  S+     Y+A + NG  +A+KKL        + RRR    LA 
Sbjct: 712 DDVVECLSKTDNILGMGST--GTVYKAEMPNGEIIAVKKLWGKNKENGKIRRRKSGVLAE 771

Query: 164 --VLASLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQ-LELKWEVRLRI 223
             VL ++RHRN++ L           L+Y+ + NGSL+D +    +      +W    +I
Sbjct: 772 VDVLGNVRHRNIVRLLGCCTNRDCTMLLYEYMPNGSLDDLLHGGDKTMTAAAEWTALYQI 831

Query: 224 AVGIARGLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSL--- 283
           A+G+A+G+ +LH +CDP I+HR+LKPSN+LLDA+FE R+AD G+  L+   D   S+   
Sbjct: 832 AIGVAQGICYLHHDCDPVIVHRDLKPSNILLDADFEARVADFGVAKLI-QTDESMSVVAG 891

Query: 284 ---YTAPECLNSSSRYTDKSDIFSFGVILGILLSSRDVMEQSSSDGEG-----------E 343
              Y APE    + +   KSDI+S+GVIL  +++ +  +E    +G             +
Sbjct: 892 SYGYIAPE-YAYTLQVDKKSDIYSYGVILLEIITGKRSVEPEFGEGNSIVDWVRSKLKTK 951

Query: 344 ETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQ 376
           E  E  +D+S+  R   +  +EM   LRIA +C S    DRP   ++LL+L +
Sbjct: 952 EDVEEVLDKSMG-RSCSLIREEMKQMLRIALLCTSRSPTDRPPMRDVLLILQE 999

BLAST of MS003006 vs. ExPASy Swiss-Prot
Match: C0LGU1 (Probable LRR receptor-like serine/threonine-protein kinase At5g37450 OS=Arabidopsis thaliana OX=3702 GN=At5g37450 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.7e-30
Identity = 96/290 (33.10%), Postives = 151/290 (52.07%), Query Frame = 0

Query: 106 LQSALRNETQLLGSSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLA 165
           L SA  + + L       + K Y+  L  GL +A+K+ +       + ++    ++ +L+
Sbjct: 624 LDSATSSFSDLSQIGRGGYGKVYKGHLPGGLVVAVKRAEQ---GSLQGQKEFFTEIELLS 683

Query: 166 SLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIAR 225
            L HRNL++L  Y  + G   LVY+ + NGSL+DA+       L L   +RLRIA+G AR
Sbjct: 684 RLHHRNLVSLLGYCDQKGEQMLVYEYMPNGSLQDALSARFRQPLSL--ALRLRIALGSAR 743

Query: 226 GLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGLINLV------LNFDRPPSL--- 285
           G+L+LH E DPPI+HR++KPSN+LLD++  P++AD G+  L+      +  D   ++   
Sbjct: 744 GILYLHTEADPPIIHRDIKPSNILLDSKMNPKVADFGISKLIALDGGGVQRDHVTTIVKG 803

Query: 286 ---YTAPECLNSSSRYTDKSDIFSFGVI-LGILLSSRDVMEQSSSDGEGEETGEAAVDRS 345
              Y  PE    S R T+KSD++S G++ L IL   R +    +   E  E  +A +  S
Sbjct: 804 TPGYVDPE-YYLSHRLTEKSDVYSLGIVFLEILTGMRPISHGRNIVREVNEACDAGMMMS 863

Query: 346 ISFREEGVGEDE-----MALALRIAGVCVSDLAEDRPSSDELLLMLTQLH 378
           +  R  G   +E     M LA+R    C  D  E RP   E++  L  ++
Sbjct: 864 VIDRSMGQYSEECVKRFMELAIR----CCQDNPEARPWMLEIVRELENIY 903

BLAST of MS003006 vs. ExPASy Swiss-Prot
Match: C0LGV1 (LRR receptor-like serine/threonine-protein kinase RGI2 OS=Arabidopsis thaliana OX=3702 GN=RGI2 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 84/270 (31.11%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 128  YRAILANGLALAIKKLQPI-------QCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 187
            Y+A + N   +A+KKL P+       + + +  R     ++  L S+RH+N++       
Sbjct: 800  YKAEMPNREVIAVKKLWPVTVPNLNEKTKSSGVRDSFSAEVKTLGSIRHKNIVRFLGCCW 859

Query: 188  EMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 247
               +  L+YD +SNGSL   + + R     L WEVR +I +G A+GL +LH +C PPI+H
Sbjct: 860  NKNTRLLMYDYMSNGSLGSLLHE-RSGVCSLGWEVRYKIILGAAQGLAYLHHDCVPPIVH 919

Query: 248  RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSL--------YTAPECLNSSSRYTDK 307
            R++K +N+L+  +FEP + D GL  LV + D   S         Y APE    S + T+K
Sbjct: 920  RDIKANNILIGPDFEPYIGDFGLAKLVDDGDFARSSNTIAGSYGYIAPE-YGYSMKITEK 979

Query: 308  SDIFSFGVILGILLSSRDVMEQSSSDG------EGEETGEAAVDRSISFREEGVGEDEMA 367
            SD++S+GV++  +L+ +  ++ +  DG        +      +D+ +  R E   E EM 
Sbjct: 980  SDVYSYGVVVLEVLTGKQPIDPTIPDGLHIVDWVKKIRDIQVIDQGLQARPESEVE-EMM 1039

Query: 368  LALRIAGVCVSDLAEDRPSSDELLLMLTQL 377
              L +A +C++ + EDRP+  ++  ML+++
Sbjct: 1040 QTLGVALLCINPIPEDRPTMKDVAAMLSEI 1066

BLAST of MS003006 vs. ExPASy TrEMBL
Match: A0A6J1D5F2 (inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017458 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.3e-205
Identity = 375/379 (98.94%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60
           MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV
Sbjct: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60

Query: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120
           ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS
Sbjct: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120

Query: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180
           SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG
Sbjct: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180

Query: 181 EMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240
           EMGSCCLVYDLV NGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH
Sbjct: 181 EMGSCCLVYDLVPNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240

Query: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGV 300
           RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLN SSRYTDKSDIFSFGV
Sbjct: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLN-SSRYTDKSDIFSFGV 300

Query: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLA 360
           ILGILLSSRDVMEQSSSDGEGEETGEAAVD SISFREEGVGEDEMALALRIAGVCVSDLA
Sbjct: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDGSISFREEGVGEDEMALALRIAGVCVSDLA 360

Query: 361 EDRPSSDELLLMLTQLHCF 380
           ED PSSDELLLMLTQLHCF
Sbjct: 361 EDXPSSDELLLMLTQLHCF 378

BLAST of MS003006 vs. ExPASy TrEMBL
Match: A0A6J1D486 (inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017458 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.3e-199
Identity = 366/379 (96.57%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60
           MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV
Sbjct: 1   MGFLMLAYITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGFQRILLSV 60

Query: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120
           ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS
Sbjct: 61  ALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSS 120

Query: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180
           SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG
Sbjct: 121 SSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVG 180

Query: 181 EMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240
           EMGSCCLVYDLV NGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH
Sbjct: 181 EMGSCCLVYDLVPNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILH 240

Query: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGV 300
           RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSS          SFGV
Sbjct: 241 RNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSS----------SFGV 300

Query: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLA 360
           ILGILLSSRDVMEQSSSDGEGEETGEAAVD SISFREEGVGEDEMALALRIAGVCVSDLA
Sbjct: 301 ILGILLSSRDVMEQSSSDGEGEETGEAAVDGSISFREEGVGEDEMALALRIAGVCVSDLA 360

Query: 361 EDRPSSDELLLMLTQLHCF 380
           ED PSSDELLLMLTQLHCF
Sbjct: 361 EDXPSSDELLLMLTQLHCF 369

BLAST of MS003006 vs. ExPASy TrEMBL
Match: A0A6J1JUG8 (inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Cucurbita maxima OX=3661 GN=LOC111488475 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.7e-141
Identity = 282/402 (70.15%), Postives = 316/402 (78.61%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFS--CGGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           MGF      TLLLLF+  FP FS   GG +T ALV SS+H VP P+T  HFKNGFQRILL
Sbjct: 1   MGFFAFTIHTLLLLFVFWFPFFSGAKGGVETDALVGSSEHFVPSPKTL-HFKNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGVVTG  GA LS WFIR+LV YLNRTPLLEGPLIFSPKID DALQSALRN+TQLLG
Sbjct: 61  SVALGVVTGLTGALLSVWFIRSLVGYLNRTPLLEGPLIFSPKIDADALQSALRNQTQLLG 120

Query: 121 --SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQ 180
             SSSSP+ K YR +LANGLA+A+K+L+PI+C +AEARRRMQ QLAVL  L+HRN+M+L+
Sbjct: 121 SSSSSSPNGKCYREVLANGLAVAVKRLEPIECGRAEARRRMQQQLAVLGGLKHRNVMSLR 180

Query: 181 AYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDP 240
           AYV EMGSCCLVYD V +GSLEDAMRKVRENQLEL WEVRLRIAVGIARGL FLHFEC+P
Sbjct: 181 AYVAEMGSCCLVYDFVPSGSLEDAMRKVRENQLELNWEVRLRIAVGIARGLQFLHFECNP 240

Query: 241 PILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIF 300
            ILH NLKPSNVLLDAEFEPRLAD GL NL+ +FD PPSLYTAPECLN SSRYTDKSDIF
Sbjct: 241 RILHCNLKPSNVLLDAEFEPRLADFGLTNLISDFDAPPSLYTAPECLN-SSRYTDKSDIF 300

Query: 301 SFGVILGILLSSRDVMEQSSSDGEGEETGEAA-----VDRSISFR--------------E 360
           SFGVIL +LL+ R+  EQ+S DG G+++G        +D+S+  R              E
Sbjct: 301 SFGVILSVLLTGRNPTEQASLDG-GDDSGSEGRLGRWLDQSVDPREALDKAIRGEGMEEE 360

Query: 361 EGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
           E   E+EM LALRIAGVC S+L EDRP SDELL MLTQL  F
Sbjct: 361 EEEEEEEMVLALRIAGVCASELPEDRPCSDELLAMLTQLQSF 399

BLAST of MS003006 vs. ExPASy TrEMBL
Match: A0A6J1GPZ9 (inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Cucurbita moschata OX=3662 GN=LOC111456060 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.7e-141
Identity = 282/401 (70.32%), Postives = 314/401 (78.30%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFS--CGGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           MGF      TLLLLF+  FP FS   GG QT ALV SS+H VP P+T  HFKNGFQRILL
Sbjct: 1   MGFFAFTIHTLLLLFVFWFPFFSGAKGGVQTDALVGSSEHFVPSPKTL-HFKNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGVVTG  GA LS WFIR+LV YLNRTPLLEGPLIFSPKID DALQSALRN+TQLLG
Sbjct: 61  SVALGVVTGLTGALLSVWFIRSLVGYLNRTPLLEGPLIFSPKIDADALQSALRNQTQLLG 120

Query: 121 ----SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMT 180
               SSSSP+ K YR +LANGL +A+K+L+PI+C +AEARRRMQ QLAVL  L+HRN+M+
Sbjct: 121 SSSSSSSSPNGKCYREVLANGLTVAVKRLEPIECGRAEARRRMQQQLAVLGGLKHRNVMS 180

Query: 181 LQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFEC 240
           L+AYV EMGSCCLVYD V +GSLEDAMRKVRENQLEL WEVRLRIAVGIARGL FLHFEC
Sbjct: 181 LRAYVAEMGSCCLVYDFVPSGSLEDAMRKVRENQLELNWEVRLRIAVGIARGLQFLHFEC 240

Query: 241 DPPILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSD 300
           +P ILH NLKPSNVLLDAEFEPRLAD GL NL+ +FD PPSLYTAPECLN SSRYTDKSD
Sbjct: 241 NPRILHGNLKPSNVLLDAEFEPRLADFGLTNLISDFDAPPSLYTAPECLN-SSRYTDKSD 300

Query: 301 IFSFGVILGILLSSRDVMEQSSSDGEGEETGEAA-----VDRSISFRE-----------E 360
           IFSFGVIL +LL+ RD  EQ+S D  G+++G        +D+S+  RE           E
Sbjct: 301 IFSFGVILSVLLTGRDPTEQASLD-RGDDSGSEGRLGRWLDQSVEPREALDKGIRGEGME 360

Query: 361 GVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
              E+EM LALRIAGVC S+L EDRP SDELL MLTQL  F
Sbjct: 361 EEEEEEMVLALRIAGVCASELPEDRPCSDELLAMLTQLQSF 398

BLAST of MS003006 vs. ExPASy TrEMBL
Match: E5GB59 (Receptor-like protein kinase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 7.0e-127
Identity = 262/400 (65.50%), Postives = 302/400 (75.50%), Query Frame = 0

Query: 1   MGFLMLAYITLLLLFILSFPLFSC--GGFQTHALVASSDHLVPFPETTSHFKNGFQRILL 60
           M F      TLL LF+ SFPLFS    G QT        H  PFP+  S F NGFQRILL
Sbjct: 1   MAFFTFNIHTLLYLFLFSFPLFSAATSGVQT--------HFPPFPK-ASRFNNGFQRILL 60

Query: 61  SVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLG 120
           SVALGV+TG   +FLS  FIRTL+ +LNRTPLL+GPLIFSPKID D+LQSAL N+T LLG
Sbjct: 61  SVALGVLTGLTASFLSVLFIRTLLRFLNRTPLLQGPLIFSPKIDADSLQSALLNQTHLLG 120

Query: 121 SSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAY 180
           SSS+P+ K Y+A+LANG  +A+KKL+PIQC + EARRRMQ Q+AVL +L+HRN+M+L+AY
Sbjct: 121 SSSNPNGKCYKALLANGFTVAVKKLEPIQCGRTEARRRMQQQMAVLGALKHRNVMSLRAY 180

Query: 181 VGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPI 240
           VGEMGSCCLVYD +  GSLEDAMRKVRENQLELKWEVRLRIAVGIARGL FLHFEC+P I
Sbjct: 181 VGEMGSCCLVYDFLPTGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLQFLHFECNPRI 240

Query: 241 LHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSF 300
           LH NLKPSN+LLDA+FEPRL D GL NL+ +F    SLYTAPECLN +SRYTDKSDI+SF
Sbjct: 241 LHCNLKPSNILLDADFEPRLVDFGLTNLIPDFHTSASLYTAPECLN-NSRYTDKSDIYSF 300

Query: 301 GVILGILLSSRDVM--EQSSSD-----GEGEETG---------EAAVDRSISFREEGV-- 360
           GVIL +LL+ RD +  EQ SSD     G G   G           A+D+SI  R EG+  
Sbjct: 301 GVILRVLLTGRDPIETEQVSSDRGDSSGSGGSLGRWVKQSVEPREALDKSI--RGEGMEE 360

Query: 361 -GEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
             E+EM LALRIAGVC S+LAEDRP SDELL+MLTQL  F
Sbjct: 361 EEEEEMVLALRIAGVCTSELAEDRPCSDELLVMLTQLQSF 388

BLAST of MS003006 vs. TAIR 10
Match: AT5G13290.2 (Protein kinase superfamily protein )

HSP 1 Score: 320.1 bits (819), Expect = 2.4e-87
Identity = 187/393 (47.58%), Postives = 258/393 (65.65%), Query Frame = 0

Query: 9   ITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGF--QRILLSVALGVVT 68
           I+LLLLF L F  FS     T     +  HL     +++  ++    + I++S+  G++T
Sbjct: 16  ISLLLLFFLVF--FSRTSTSTSCRRRTVKHLSTTSTSSTPLESRITSKVIVISIVSGILT 75

Query: 69  GFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQLLGSSSSPHAK 128
           G   A + A+ +R++V ++ +TP+L+GP++FSPKI P +L +AL N  QLLGS    + K
Sbjct: 76  GLVSALVLAFLVRSIVKFMKQTPILKGPVVFSPKITPKSLHAALSNGIQLLGSDL--NGK 135

Query: 129 YYRAILANGLALAIKKL-------QPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYV 188
           YY+ +L NGL +A+K+L        P        +RR+Q +L +LA LRHRNLM+L+AYV
Sbjct: 136 YYKMVLDNGLVVAVKRLGSLEGVGSPESSSSKSVKRRLQKELELLAGLRHRNLMSLRAYV 195

Query: 189 GEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPIL 248
            E     LVYD + NGSLED M KVR  ++EL WE+RLR+AVGI +GL +LHF C+  IL
Sbjct: 196 RESDEFSLVYDYMPNGSLEDVMNKVRTKEVELGWEIRLRVAVGIVKGLQYLHFSCETQIL 255

Query: 249 HRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFG 308
           H NLKP+NV+LD+EFEPRLADCGL  ++ +     S Y+APE  + S+RYTDKSDIFSFG
Sbjct: 256 HYNLKPTNVMLDSEFEPRLADCGLAKIMPSSHTAVSCYSAPES-SQSNRYTDKSDIFSFG 315

Query: 309 VILGILLSSRD----VMEQSSSDG-------EGEETGEA--AVDRSISFREEGVGEDEMA 368
           +ILG+LL+ RD      E+S+S G         +++GEA  A+D++I   E  V EDEM 
Sbjct: 316 MILGVLLTGRDPTHPFCEESASGGSLGQWLKHLQQSGEAREALDKTILGEE--VEEDEML 375

Query: 369 LALRIAGVCVSDLAEDRPSSDELLLMLTQLHCF 380
           +ALRI  +C+SD   DRPSSDEL+ MLTQLH F
Sbjct: 376 MALRITIICLSDFPADRPSSDELVHMLTQLHSF 401

BLAST of MS003006 vs. TAIR 10
Match: AT5G13290.1 (Protein kinase superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-78
Identity = 175/388 (45.10%), Postives = 245/388 (63.14%), Query Frame = 0

Query: 9   ITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGF--QRILLSVALGVVT 68
           I+LLLLF L F  FS     T     +  HL     +++  ++    + I++S+  G++T
Sbjct: 16  ISLLLLFFLVF--FSRTSTSTSCRRRTVKHLSTTSTSSTPLESRITSKVIVISIVSGILT 75

Query: 69  GFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQL--LGSSSSPH 128
           G   A + A+ +R++V ++ +TP+L+GP++FSPKI P +L +AL N  +L  L    SP 
Sbjct: 76  GLVSALVLAFLVRSIVKFMKQTPILKGPVVFSPKITPKSLHAALSNVKRLGSLEGVGSPE 135

Query: 129 AKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVGEMGS 188
           +   +++                      +RR+Q +L +LA LRHRNLM+L+AYV E   
Sbjct: 136 SSSSKSV----------------------KRRLQKELELLAGLRHRNLMSLRAYVRESDE 195

Query: 189 CCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILHRNLK 248
             LVYD + NGSLED M KVR  ++EL WE+RLR+AVGI +GL +LHF C+  ILH NLK
Sbjct: 196 FSLVYDYMPNGSLEDVMNKVRTKEVELGWEIRLRVAVGIVKGLQYLHFSCETQILHYNLK 255

Query: 249 PSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSSSRYTDKSDIFSFGVILGI 308
           P+NV+LD+EFEPRLADCGL  ++ +     S Y+APE  + S+RYTDKSDIFSFG+ILG+
Sbjct: 256 PTNVMLDSEFEPRLADCGLAKIMPSSHTAVSCYSAPES-SQSNRYTDKSDIFSFGMILGV 315

Query: 309 LLSSRD----VMEQSSSDG-------EGEETGEA--AVDRSISFREEGVGEDEMALALRI 368
           LL+ RD      E+S+S G         +++GEA  A+D++I   E  V EDEM +ALRI
Sbjct: 316 LLTGRDPTHPFCEESASGGSLGQWLKHLQQSGEAREALDKTILGEE--VEEDEMLMALRI 375

Query: 369 AGVCVSDLAEDRPSSDELLLMLTQLHCF 380
             +C+SD   DRPSSDEL+ MLTQLH F
Sbjct: 376 TIICLSDFPADRPSSDELVHMLTQLHSF 376

BLAST of MS003006 vs. TAIR 10
Match: AT5G13290.3 (Protein kinase superfamily protein )

HSP 1 Score: 208.0 bits (528), Expect = 1.3e-53
Identity = 118/282 (41.84%), Postives = 172/282 (60.99%), Query Frame = 0

Query: 9   ITLLLLFILSFPLFSCGGFQTHALVASSDHLVPFPETTSHFKNGF--QRILLSVALGVVT 68
           I+LLLLF L F  FS     T     +  HL     +++  ++    + I++S+  G++T
Sbjct: 16  ISLLLLFFLVF--FSRTSTSTSCRRRTVKHLSTTSTSSTPLESRITSKVIVISIVSGILT 75

Query: 69  GFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNETQL--LGSSSSPH 128
           G   A + A+ +R++V ++ +TP+L+GP++FSPKI P +L +AL N  +L  L    SP 
Sbjct: 76  GLVSALVLAFLVRSIVKFMKQTPILKGPVVFSPKITPKSLHAALSNVKRLGSLEGVGSPE 135

Query: 129 AKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLAVLASLRHRNLMTLQAYVGEMGS 188
           +   +++                      +RR+Q +L +LA LRHRNLM+L+AYV E   
Sbjct: 136 SSSSKSV----------------------KRRLQKELELLAGLRHRNLMSLRAYVRESDE 195

Query: 189 CCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLRIAVGIARGLLFLHFECDPPILHRNLK 248
             LVYD + NGSLED M KVR  ++EL WE+RLR+AVGI +GL +LHF C+  ILH NLK
Sbjct: 196 FSLVYDYMPNGSLEDVMNKVRTKEVELGWEIRLRVAVGIVKGLQYLHFSCETQILHYNLK 255

Query: 249 PSNVLLDAEFEPRLADCGLINLVLNFDRPPSLYTAPECLNSS 287
           P+NV+LD+EFEPRLADCGL  ++ +     S Y+APE   S+
Sbjct: 256 PTNVMLDSEFEPRLADCGLAKIMPSSHTAVSCYSAPESSQSN 273

BLAST of MS003006 vs. TAIR 10
Match: AT2G31880.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.0e-32
Identity = 108/358 (30.17%), Postives = 181/358 (50.56%), Query Frame = 0

Query: 57  LLSVALGVVTGFAGAFLSAWFIRTLVSYLNRTPLLEGPLIFSPKIDPDALQSALRNE--- 116
           +L   +G + G    F+ +   + ++  +  +    GP IFSP I      + L NE   
Sbjct: 288 ILGFVVGAIGGTISGFVFSVLFKLIIQAIRGSEKPPGPSIFSPLIKKAEDLAFLENEEAL 347

Query: 117 --TQLLGSSSSPHAKYYRAIL--ANGLALAIKK-LQPIQCRKA----------EARRRMQ 176
              +++G       + ++A L  +NG  +A+KK +QP +              +  R+++
Sbjct: 348 ASLEIIGRGGC--GEVFKAELPGSNGKIIAVKKVIQPPKDADELTDEDSKFLNKKMRQIR 407

Query: 177 HQLAVLASLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQLELKWEVRLR 236
            ++  +  +RHRNL+ L A+V       LVY+ +  GSL+D +  V+    EL W  R +
Sbjct: 408 SEINTVGHIRHRNLLPLLAHVSRPECHYLVYEYMEKGSLQDILTDVQAGNQELMWPARHK 467

Query: 237 IAVGIARGLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGL--------INLVLNF 296
           IA+GIA GL +LH + +P I+HR+LKP+NVLLD + E R++D GL         ++  + 
Sbjct: 468 IALGIAAGLEYLHMDHNPRIIHRDLKPANVLLDDDMEARISDFGLAKAMPDAVTHITTSH 527

Query: 297 DRPPSLYTAPECLNSSSRYTDKSDIFSFGVILGILLSSR---DVMEQSSSDGE------- 356
                 Y APE    + ++TDK DI+SFGVILGIL+  +   D   Q + +         
Sbjct: 528 VAGTVGYIAPE-FYQTHKFTDKCDIYSFGVILGILVIGKLPSDEFFQHTDEMSLIKWMRN 587

Query: 357 --GEETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQL 377
               E    A+D  +   ++G  +++M L L+IA  C  D  + RP+S ++  ML+Q+
Sbjct: 588 IITSENPSLAIDPKL--MDQGF-DEQMLLVLKIACYCTLDDPKQRPNSKDVRTMLSQI 639

BLAST of MS003006 vs. TAIR 10
Match: AT5G61480.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 136.0 bits (341), Expect = 6.5e-32
Identity = 98/293 (33.45%), Postives = 153/293 (52.22%), Query Frame = 0

Query: 104 DALQSALRNETQLLGSSSSPHAKYYRAILANGLALAIKKLQPIQCRKAEARRRMQHQLA- 163
           D +   L     +LG  S+     Y+A + NG  +A+KKL        + RRR    LA 
Sbjct: 712 DDVVECLSKTDNILGMGST--GTVYKAEMPNGEIIAVKKLWGKNKENGKIRRRKSGVLAE 771

Query: 164 --VLASLRHRNLMTLQAYVGEMGSCCLVYDLVSNGSLEDAMRKVRENQ-LELKWEVRLRI 223
             VL ++RHRN++ L           L+Y+ + NGSL+D +    +      +W    +I
Sbjct: 772 VDVLGNVRHRNIVRLLGCCTNRDCTMLLYEYMPNGSLDDLLHGGDKTMTAAAEWTALYQI 831

Query: 224 AVGIARGLLFLHFECDPPILHRNLKPSNVLLDAEFEPRLADCGLINLVLNFDRPPSL--- 283
           A+G+A+G+ +LH +CDP I+HR+LKPSN+LLDA+FE R+AD G+  L+   D   S+   
Sbjct: 832 AIGVAQGICYLHHDCDPVIVHRDLKPSNILLDADFEARVADFGVAKLI-QTDESMSVVAG 891

Query: 284 ---YTAPECLNSSSRYTDKSDIFSFGVILGILLSSRDVMEQSSSDGEG-----------E 343
              Y APE    + +   KSDI+S+GVIL  +++ +  +E    +G             +
Sbjct: 892 SYGYIAPE-YAYTLQVDKKSDIYSYGVILLEIITGKRSVEPEFGEGNSIVDWVRSKLKTK 951

Query: 344 ETGEAAVDRSISFREEGVGEDEMALALRIAGVCVSDLAEDRPSSDELLLMLTQ 376
           E  E  +D+S+  R   +  +EM   LRIA +C S    DRP   ++LL+L +
Sbjct: 952 EDVEEVLDKSMG-RSCSLIREEMKQMLRIALLCTSRSPTDRPPMRDVLLILQE 999

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148904.12.7e-20598.94inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X1 [Mom... [more]
XP_022148905.11.1e-19896.57inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X2 [Mom... [more]
KAG7014619.17.2e-14270.57Inactive leucine-rich repeat receptor-like protein kinase CORYNE [Cucurbita argy... [more]
KAG6576100.19.4e-14270.75Inactive leucine-rich repeat receptor-like protein kinase CORYNE, partial [Cucur... [more]
XP_022953555.13.6e-14170.32inactive leucine-rich repeat receptor-like protein kinase CORYNE [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
Q9LYU73.4e-8647.58Inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Arabidopsis ... [more]
Q9SKB21.4e-3130.17Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR... [more]
Q9FII59.1e-3133.45Leucine-rich repeat receptor-like protein kinase TDR OS=Arabidopsis thaliana OX=... [more]
C0LGU17.7e-3033.10Probable LRR receptor-like serine/threonine-protein kinase At5g37450 OS=Arabidop... [more]
C0LGV11.3e-2931.11LRR receptor-like serine/threonine-protein kinase RGI2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1D5F21.3e-20598.94inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X1 OS=M... [more]
A0A6J1D4865.3e-19996.57inactive leucine-rich repeat receptor-like protein kinase CORYNE isoform X2 OS=M... [more]
A0A6J1JUG81.7e-14170.15inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Cucurbita ma... [more]
A0A6J1GPZ91.7e-14170.32inactive leucine-rich repeat receptor-like protein kinase CORYNE OS=Cucurbita mo... [more]
E5GB597.0e-12765.50Receptor-like protein kinase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G13290.22.4e-8747.58Protein kinase superfamily protein [more]
AT5G13290.11.6e-7845.10Protein kinase superfamily protein [more]
AT5G13290.31.3e-5341.84Protein kinase superfamily protein [more]
AT2G31880.11.0e-3230.17Leucine-rich repeat protein kinase family protein [more]
AT5G61480.16.5e-3233.45Leucine-rich repeat protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 99..191
e-value: 1.6E-9
score: 39.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 192..378
e-value: 2.5E-36
score: 126.9
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 160..303
e-value: 0.0026
score: 14.6
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 98..313
e-value: 3.1E-6
score: 24.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 313..332
NoneNo IPR availablePANTHERPTHR48005LEUCINE RICH REPEAT KINASE 2coord: 10..377
NoneNo IPR availablePANTHERPTHR48005:SF21INACTIVE LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE CORYNEcoord: 10..377
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 127..322
e-value: 1.2E-25
score: 90.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 111..379
score: 25.248283
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 126..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS003006.1MS003006.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity