MS002851 (gene) Bitter gourd (TR) v1

Overview
NameMS002851
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationscaffold359: 208092 .. 208853 (+)
RNA-Seq ExpressionMS002851
SyntenyMS002851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCTAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATCCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCAAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCTGTTCTAAACTCAGGTCTT

mRNA sequence

AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCTAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATCCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCAAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCTGTTCTAAACTCAGGTCTT

Coding sequence (CDS)

AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCTAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATCCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCAAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCTGTTCTAAACTCAGGTCTT

Protein sequence

RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGTKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNCVSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHNLVVDGGFSVLNSGL
Homology
BLAST of MS002851 vs. NCBI nr
Match: XP_022142634.1 (secoisolariciresinol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.5e-138
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LVVDGGFSVLNSGL 255
           LVVDGGFSVLNSGL
Sbjct: 252 LVVDGGFSVLNSGL 265

BLAST of MS002851 vs. NCBI nr
Match: XP_038895162.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 420.2 bits (1079), Expect = 1.2e-113
Identity = 207/255 (81.18%), Postives = 229/255 (89.80%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSNH+GSSS SFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIEDDLGHSLSNHIGSSSFSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN VD+ +S YGKLDIMFNNAAILGAP +NILEN+SSDFQKVLNVNL GAFLGT
Sbjct: 72  TKEKDVENAVDSALSDYGKLDIMFNNAAILGAPKFNILENESSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR MIPA RGSIIT  S  +++GGI PHACTSSKHGV+GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARVMIPAGRGSIITTGSVISSIGGIGPHACTSSKHGVVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLK-NEDAISEEDVAEAALYLGSDAAKYVSGH 240
           VSPY V T++TR+L ++KDGD+ P++YWNLK  ED + EEDVAEAALYL SDA+KYVSGH
Sbjct: 192 VSPYGVHTKMTRELFKVKDGDEIPSLYWNLKCGEDVVKEEDVAEAALYLASDASKYVSGH 251

Query: 241 NLVVDGGFSVLNSGL 255
           NLVVDGGF+V+N GL
Sbjct: 252 NLVVDGGFTVVNQGL 266

BLAST of MS002851 vs. NCBI nr
Match: XP_022142407.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 392.1 bits (1006), Expect = 3.6e-105
Identity = 196/251 (78.09%), Postives = 219/251 (87.25%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSN+L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T+E DV N VDTTVSKYGKLDIMFNNA I G   +NILEN+S DFQKVLNVNL GAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PARRGSII  AS  + +GG  PHA TSSKHG++GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VPTQ+TRDL +L+DGD+FPN Y +LK    + EED+AEAALYLGSDA+K+VSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LVVDGGFSVLN 252
           L+VDGGF+V+N
Sbjct: 252 LIVDGGFTVVN 262

BLAST of MS002851 vs. NCBI nr
Match: XP_022927563.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 377.9 bits (969), Expect = 7.0e-101
Identity = 187/253 (73.91%), Postives = 214/253 (84.58%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILEN+SSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +TR+L +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFSVLNSG 254
           L+VDGGF+  N G
Sbjct: 252 LIVDGGFTAANQG 264

BLAST of MS002851 vs. NCBI nr
Match: XP_023520696.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.9 bits (969), Expect = 7.0e-101
Identity = 186/253 (73.52%), Postives = 213/253 (84.19%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADIQDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILENDSSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENDSSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +T++L +L+DGD+FPN+Y + K  D +  ED+AE ALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDMTKELFKLEDGDEFPNVYSHFKCGDILRAEDIAETALYLASDASRFVSGHN 251

Query: 241 LVVDGGFSVLNSG 254
           L+VDGGF+  N G
Sbjct: 252 LIVDGGFTAANQG 264

BLAST of MS002851 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.4e-75
Identity = 146/255 (57.25%), Postives = 189/255 (74.12%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGA GIGE TA+LF +HGAKV IAD+ D+LG S+   +G+S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E  V+N VD TVS YGKLDIMF+NA I       I++N+ +DF++V +VN+ G FL  
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR MIPAR G+II+ AS ++T+GG + HA   SKH VLGL +N AV+LG++GIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMY---WNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
           +SP+ +PT L +    +K+ ++F N+     NLK     + EDVA AALYL SD AKYVS
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKG-PKFNVEDVANAALYLASDEAKYVS 253

Query: 241 GHNLVVDGGFSVLNS 253
           GHNL +DGGFSV NS
Sbjct: 254 GHNLFIDGGFSVCNS 267

BLAST of MS002851 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.2e-72
Identity = 144/254 (56.69%), Postives = 179/254 (70.47%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           +L GKVA+ITGGA GIG  TARLF KHGA+VV+ADI D+LG SL   LG  +SS+VHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E DV   VD  V+++GKLD+MFNNA + G P + + E    DF++VL VNL G FLGT
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGT 133

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR M PARRGSII+ AS +++V G A HA T+SKH ++G  +NAA +LGR+GIRVNC
Sbjct: 134 KHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVNC 193

Query: 181 VSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSG 240
           VSP  V T L R  + + D   +       NLK   A+  +D+A AAL+L SD  +YVSG
Sbjct: 194 VSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVSG 253

Query: 241 HNLVVDGGFSVLNS 253
            NL VDGG SV+NS
Sbjct: 254 QNLRVDGGLSVVNS 267

BLAST of MS002851 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.4e-67
Identity = 136/255 (53.33%), Postives = 181/255 (70.98%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSS-SSFVHCD 60
           RL+ KVAIITGGA GIGE TA+LF ++GAKVVIADI DD GQ + N++GS    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VTK++DV N+VDTT++K+GKLDIMF N  +L    Y+ILE  + DF++V+++N++GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPARRGSII-TAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRV 180
            KHAAR MIPA++GSI+ TA+ ++ T G    H  T++KH VLGL  +   +LG YGIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NCVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYV 240
           NCVSPY V + L  D+  +     ++  +   NLK    +  EDVA+A  YL  D +KYV
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKG-TLLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLVVDGGFSVLN 252
           SG NLV+DGG++  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

BLAST of MS002851 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.0e-67
Identity = 135/255 (52.94%), Postives = 183/255 (71.76%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSS-SSFVHCD 60
           RL+ KVAIITGGA GIGE TA+LF ++GAKVVIADI+DD GQ + N++GS    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VTK++DV N+VDTT++K+GKLDIMF N  +L    Y+ILE  + DF++V+++N++GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPARRGSII-TAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRV 180
            KHAAR MIPA++GSI+ TA+ ++ T G    H  T++KH VLGL  +   +LG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NCVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYV 240
           NCVSPY V + L  D+  +     ++  +   NLK    +  EDVA+A  YL  D +KYV
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKG-ILLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLVVDGGFSVLN 252
           SG NLV+DGG++  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

BLAST of MS002851 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.9e-67
Identity = 136/263 (51.71%), Postives = 174/263 (66.16%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSS-SSSFVHCD 60
           RLEGKVA++TGGA GIGE  ARLF +HGAK+ I D+ D+LGQ +S  LG    + + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VT E DV   VD T  KYG +DIM NNA I G    +I + D ++F+KV ++N+ G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 121 TKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
            KHAAR MIP  +GSI++ AS ++ + G  PH  T +KH V+GL K+ A +LGR+GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 181 CVSPYAVPTQLTRDLL--------RLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSD 240
           CVSPYAVPT+L+   L         L+    F     NLK  D +   DVAEA LYL ++
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVD-LMPNDVAEAVLYLATE 241

Query: 241 AAKYVSGHNLVVDGGFSVLNSGL 255
            +KYVSG NLV+DGGFS+ N  L
Sbjct: 242 ESKYVSGLNLVIDGGFSIANHTL 263

BLAST of MS002851 vs. ExPASy TrEMBL
Match: A0A6J1CM16 (secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111012697 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.0e-139
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LVVDGGFSVLNSGL 255
           LVVDGGFSVLNSGL
Sbjct: 252 LVVDGGFSVLNSGL 265

BLAST of MS002851 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.7e-105
Identity = 196/251 (78.09%), Postives = 219/251 (87.25%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSN+L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T+E DV N VDTTVSKYGKLDIMFNNA I G   +NILEN+S DFQKVLNVNL GAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PARRGSII  AS  + +GG  PHA TSSKHG++GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VPTQ+TRDL +L+DGD+FPN Y +LK    + EED+AEAALYLGSDA+K+VSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LVVDGGFSVLN 252
           L+VDGGF+V+N
Sbjct: 252 LIVDGGFTVVN 262

BLAST of MS002851 vs. ExPASy TrEMBL
Match: A0A6J1ELC4 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111434357 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.4e-101
Identity = 187/253 (73.91%), Postives = 214/253 (84.58%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILEN+SSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +TR+L +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFSVLNSG 254
           L+VDGGF+  N G
Sbjct: 252 LIVDGGFTAANQG 264

BLAST of MS002851 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 7.6e-101
Identity = 186/254 (73.23%), Postives = 216/254 (85.04%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LF KHGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKD+EN V+T +SKYGKLDIM NNA I G   +NILEN+ SDFQKVLNVNL GAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAA+AMIPAR+GSII  AS  + +GGI P+A TSSKHG+LGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+Q+TRDL +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFSVLNSGL 255
           L+VDGGF+  N GL
Sbjct: 252 LIVDGGFTSANQGL 265

BLAST of MS002851 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 7.6e-101
Identity = 186/254 (73.23%), Postives = 216/254 (85.04%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LF KHGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKD+EN V+T +SKYGKLDIM NNA I G   +NILEN+ SDFQKVLNVNL GAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAA+AMIPAR+GSII  AS  + +GGI P+A TSSKHG+LGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+Q+TRDL +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFSVLNSGL 255
           L+VDGGF+  N GL
Sbjct: 252 LIVDGGFTSANQGL 265

BLAST of MS002851 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.3e-65
Identity = 136/264 (51.52%), Postives = 179/264 (67.80%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADIND----DLGQSLSNHLGSSSSSFV 60
           RLEGKVAIITGGA GIG+ T  LF +HGA VVIAD+++     L +SLS+H  S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILG--APNYNILENDSSDFQKVLNVNLF 120
            CDV+ E DVEN+V+ TV++YG+LDI+FNNA +LG    + +IL+ D+ +F  V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GAFLGTKHAARAMIP-ARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGR 180
           G  LG KH ARAMI    +G II+ AS A  +GG+ PHA T+SKH ++GL KNAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 YGIRVNCVSPYAVPTQLTRDLLRLKDG-----------DKFPNMYWNLKNEDAISEEDVA 240
           YGIRVNC+SP+ V T +  +  R   G           ++F     NLK E  +   D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGE-TLRANDIA 270

Query: 241 EAALYLGSDAAKYVSGHNLVVDGG 247
           EAALYL SD +KYV+GHNLVVDGG
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGG 293

BLAST of MS002851 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.2e-60
Identity = 124/264 (46.97%), Postives = 167/264 (63.26%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLG----QSLSNHLGSSSSSFV 60
           RL GKVA+ITGGA GIGE   RLF KHGAKV I D+ DDLG    +SL       ++ F+
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGA 120
           H DV  E D+ N VD  V  +G LDI+ NNA + GAP  +I     S+F+   +VN+ GA
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGTKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGI 180
           FL  KHAAR MIP ++GSI++  S    VGG+ PH+   SKH VLGL ++ A +LG++GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNCVSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDA---------ISEEDVAEAALY 240
           RVNCVSPYAV T+L   L  L + ++  + +   +N  A         ++ +DVA A L+
Sbjct: 197 RVNCVSPYAVATKLA--LAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLF 256

Query: 241 LGSDAAKYVSGHNLVVDGGFSVLN 252
           L SD ++Y+SG NL++DGGF+  N
Sbjct: 257 LASDDSRYISGDNLMIDGGFTCTN 278

BLAST of MS002851 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 5.4e-59
Identity = 125/252 (49.60%), Postives = 168/252 (66.67%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+AIITGGA GIG    RLF  HGAKVVI D  ++LGQ+++  +G   +SF  CDV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T EK+VEN V  TV KYGKLD++F+NA ++  P  + L+ +   F + + VN+ GA    
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPG-SFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMI-PARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+   S A+ +GG  PHA T+SKH +LGL+K+A   LG+YGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 181 CVSPYAVPTQL-TRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSG 240
            V+PYAV T + +RD   ++  +++      LK    +    VAEAAL+L SD + YVSG
Sbjct: 185 GVAPYAVATAINSRDEETVRMVEEYSAATGILKGV-VLKARHVAEAALFLASDDSAYVSG 244

Query: 241 HNLVVDGGFSVL 251
            NL VDGG+SV+
Sbjct: 245 QNLAVDGGYSVV 254

BLAST of MS002851 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 9.3e-59
Identity = 121/253 (47.83%), Postives = 171/253 (67.59%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+ IITGGA GIG  + RLF +HGA+VVI D+ D+LGQ+++  +G   +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E +VEN V  TV KYGKLD++F+NA ++  P  +IL+ + ++  + + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTAAFI 124

Query: 121 KHAARAMI-PARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+   S AA + G APH  T+SKHG+LGL+K+A+  LG+YGIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 181 CVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
            V+P+ V T L  +  +++    ++  +   NLK    +    VAEAAL+L SD + YVS
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKG-IVLKARHVAEAALFLASDESAYVS 244

Query: 241 GHNLVVDGGFSVL 251
           G NL VDGG+SV+
Sbjct: 245 GQNLAVDGGYSVV 255

BLAST of MS002851 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.1e-58
Identity = 125/253 (49.41%), Postives = 170/253 (67.19%), Query Frame = 0

Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+AIITGGA GIG    RLF  HGAKVVI DI ++LGQ+L+  +G   +SF  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E DVEN V  TV K+GKLD++F+NA +L A   ++L+ D   F + + VN+ GA    
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMIPA-RRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAAR+M+ +  RGSI+   S AA +GG  PH+ T+SKH +LGL+++A   LG+YGIRVN
Sbjct: 125 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 184

Query: 181 CVSPYAVPTQLTR--DLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
            V+PY V T +T   +   +K  +++     NLK    +    +AEAAL+L SD + Y+S
Sbjct: 185 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGV-VLKARHIAEAALFLASDDSVYIS 244

Query: 241 GHNLVVDGGFSVL 251
           G NLVVDGGFSV+
Sbjct: 245 GQNLVVDGGFSVV 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142634.11.5e-138100.00secoisolariciresinol dehydrogenase-like [Momordica charantia][more]
XP_038895162.11.2e-11381.18secoisolariciresinol dehydrogenase-like [Benincasa hispida][more]
XP_022142407.13.6e-10578.09momilactone A synthase-like [Momordica charantia][more]
XP_022927563.17.0e-10173.91secoisolariciresinol dehydrogenase-like [Cucurbita moschata][more]
XP_023520696.17.0e-10173.52secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q94KL71.4e-7557.25Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE14.2e-7256.69Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
Q94KL82.4e-6753.33Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
A3F5F04.0e-6752.94Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
F1SWA06.9e-6751.71Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CM167.0e-139100.00secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1CMN71.7e-10578.09momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ... [more]
A0A6J1ELC43.4e-10173.91secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1EID07.6e-10173.23secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1EI147.6e-10173.23secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT3G51680.11.3e-6551.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G52340.12.2e-6046.97NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47130.15.4e-5949.60NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.19.3e-5947.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.22.1e-5849.41NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 78..89
score: 51.35
coord: 127..143
score: 32.25
coord: 174..191
score: 40.89
coord: 6..23
score: 48.82
coord: 153..172
score: 31.98
coord: 211..231
score: 25.28
NoneNo IPR availableGENE3D3.40.50.720coord: 1..252
e-value: 5.3E-75
score: 253.9
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 11..248
e-value: 1.6E-63
score: 214.4
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 2..250
e-value: 1.0E-19
score: 68.6
NoneNo IPR availablePANTHERPTHR43180:SF30MOMILACTONE A SYNTHASEcoord: 1..252
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 1..252
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 3..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002851.1MS002851.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity