Homology
BLAST of MS002839 vs. NCBI nr
Match:
XP_022142510.1 (nicalin-1 [Momordica charantia] >XP_022142511.1 nicalin-1 [Momordica charantia])
HSP 1 Score: 1069.7 bits (2765), Expect = 8.7e-309
Identity = 541/544 (99.45%), Postives = 541/544 (99.45%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKK LGGLLFLLPKIFGSDDTKN
Sbjct: 61 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKXLGGLLFLLPKIFGSDDTKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT
Sbjct: 121 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL
Sbjct: 181 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISN RVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA
Sbjct: 361 NISNLRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSR PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRMPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV
Sbjct: 481 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. NCBI nr
Match:
KAG7012715.1 (Nicalin [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1013.4 bits (2619), Expect = 7.3e-292
Identity = 508/544 (93.38%), Postives = 521/544 (95.77%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTVVI PLCELNITFVKECISQKKRLGGLLFLLPKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKELLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RL ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLVLLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. NCBI nr
Match:
XP_022945431.1 (nicalin-1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1011.5 bits (2614), Expect = 2.8e-291
Identity = 505/544 (92.83%), Postives = 522/544 (95.96%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTV+I PLCELNITFVKECISQKKRLGGLLFL+PKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVIITPLCELNITFVKECISQKKRLGGLLFLVPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
R+ ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RIHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLVLLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. NCBI nr
Match:
KAG6573634.1 (Nicalin, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1011.5 bits (2614), Expect = 2.8e-291
Identity = 507/544 (93.20%), Postives = 521/544 (95.77%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTVVI PLCELNITFVKECISQKKRLGGLLFLLPKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSG VALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGSVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RL ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLVLLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. NCBI nr
Match:
XP_023542146.1 (nicalin-1-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1009.6 bits (2609), Expect = 1.1e-290
Identity = 506/544 (93.01%), Postives = 522/544 (95.96%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTVVI PLCELNITFVKECISQKKRLGGLLFLLPKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVVITPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RL ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAVNPTYIRSWLDLLS+TPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSQTPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKL+IYQVASVTFDLLLLL LGSYLVLLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLNIYQVASVTFDLLLLLALGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. ExPASy Swiss-Prot
Match:
Q5XIA1 (Nicalin OS=Rattus norvegicus OX=10116 GN=Ncln PE=2 SV=1)
HSP 1 Score: 258.8 bits (660), Expect = 1.4e-67
Identity = 182/546 (33.33%), Postives = 290/546 (53.11%), Query Frame = 0
Query: 29 ALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPPGAD-LSRT 88
A++ ++ + DAA VYR+ QYD+ G P+G+R A LN A ++ AD LSR
Sbjct: 28 AVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTV----DADVLSRR 87
Query: 89 VVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKNDGTKCPNNGEGTVKNLLG 148
V++ L + + ++ + Q G ++ +LP+ + + V+ +
Sbjct: 88 CVLMRLLDFSYEHYQKALRQS--AGAVVIILPRAMAAVP------------QDVVRQFM- 147
Query: 149 ELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATG--------QLATATTGGYKLVV 208
E+E ++ PVYFA E E + ++ + A+ L TAT G+++V
Sbjct: 148 EIEPEMLAMETVVPVYFAVEDEALLSIYEQTQAASASQGSASAAEVLLHTATANGFQMVT 207
Query: 209 SAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPELSVGSDSNGSG 268
S A+ + + IT+++G L GL + LPTI IVA YD FG AP LS+G+DSNGSG
Sbjct: 208 SGAQSQAVSDWLITSVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSLGADSNGSG 267
Query: 269 IVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SFDHR----LRES 328
I LLE+ARLFS LY+ +T YNLLF + GG +NY GT +WL+ S DH L+++
Sbjct: 268 ISVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDSLDHTDSSLLQDN 327
Query: 329 IDYAICLNSIGSWDDKLWLHVSKPP-----ENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 388
+ + +CL+++G L LHVSKPP ++V+++++ ++ D+ F + HKKI
Sbjct: 328 VAFVLCLDTVGR-GSHLRLHVSKPPREGTLQHVFLRELEMVAAHQFPDVSF--SMVHKKI 387
Query: 389 NISNPRVAWEHEQFSRLRVTAATLSELS---AAPELLERTGGLVDNRLFLNESAIAKSIK 448
N+++ +AWEHE+F+ R+ A TLS L A P ++D R ++ + ++ +
Sbjct: 388 NLADDVLAWEHERFAIRRLPAFTLSHLENHRAGPR-----SSIMDVRSRVDSKTLTRNTR 447
Query: 449 LVAESLARHIYRYEGK----NIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDP 508
++AE+L R IY K ++ VF + + V I S +D L+ PR A L KD
Sbjct: 448 IIAEALTRVIYNLTEKGTPPDMPVFTE--QMQVQQEQIDSVMDWLTNQPRAAQLLDKDGT 507
Query: 509 FILALKKELEVHTHDVSMQH---EAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGS 546
F+ L+ L + DV H + D F FYD ++ Y+V FDLLL L +G+
Sbjct: 508 FLSTLEHFLSRYLKDVRQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLLALCIGA 540
BLAST of MS002839 vs. ExPASy Swiss-Prot
Match:
Q8VCM8 (Nicalin OS=Mus musculus OX=10090 GN=Ncln PE=1 SV=2)
HSP 1 Score: 257.7 bits (657), Expect = 3.1e-67
Identity = 181/546 (33.15%), Postives = 290/546 (53.11%), Query Frame = 0
Query: 29 ALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPPGAD-LSRT 88
A++ ++ + DAA VYR+ QYD+ G P+G+R A LN A ++ AD LSR
Sbjct: 28 AVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTV----DADVLSRR 87
Query: 89 VVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKNDGTKCPNNGEGTVKNLLG 148
V++ L + + ++ + Q G ++ +LP+ + + V+ +
Sbjct: 88 CVLMRLLDFSYEHYQKALRQS--AGAVVIILPRAMAAVP------------QDVVRQFM- 147
Query: 149 ELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATG--------QLATATTGGYKLVV 208
E+E ++ PVYFA E E + ++ + A+ L TAT G+++V
Sbjct: 148 EIEPEMLAMETVVPVYFAVEDEALLSIYEQTQAASASQGSASAAEVLLHTATANGFQMVT 207
Query: 209 SAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPELSVGSDSNGSG 268
S A+ + + IT+++G L GL + LPTI IVA YD FG AP LS+G+DSNGSG
Sbjct: 208 SGAQSQAVSDWLITSVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSLGADSNGSG 267
Query: 269 IVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SFDHR----LRES 328
I LLE+ARLFS LY+ +T YNLLF + GG +NY GT +WL+ S DH L+++
Sbjct: 268 ISVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDSLDHTDSSLLQDN 327
Query: 329 IDYAICLNSIGSWDDKLWLHVSKPP-----ENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 388
+ + +CL+++G L LHVSKPP ++ +++++ ++ D+ F + HKKI
Sbjct: 328 VAFVLCLDTVGR-GSHLRLHVSKPPREGTLQHAFLRELETVAAHQFPDVSF--SMVHKKI 387
Query: 389 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTG---GLVDNRLFLNESAIAKSIK 448
N+++ +AWEHE+F+ R+ A TLS L + R G ++D R ++ + ++ +
Sbjct: 388 NLADDVLAWEHERFAIRRLPAFTLSHLES-----HRAGPRSSIMDVRSRVDSKTLTRNTR 447
Query: 449 LVAESLARHIYRYEGK----NIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDP 508
++AE+L R IY K ++ VF + + V I S +D L+ PR A L KD
Sbjct: 448 IIAEALTRVIYNLTEKGTPPDMPVFTE--QMQVQEEQIDSVMDWLTNQPRAAQLLDKDGT 507
Query: 509 FILALKKELEVHTHDVSMQH---EAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGS 546
F+ L+ L + DV H + D F FYD ++ Y+V FDLLL L +G+
Sbjct: 508 FLSTLEHFLSRYLKDVRQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLLALCIGA 540
BLAST of MS002839 vs. ExPASy Swiss-Prot
Match:
Q6NZ07 (Nicalin-1 OS=Danio rerio OX=7955 GN=ncl1 PE=2 SV=1)
HSP 1 Score: 256.5 bits (654), Expect = 6.9e-67
Identity = 188/571 (32.92%), Postives = 300/571 (52.54%), Query Frame = 0
Query: 19 EVLESF----YPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAG 78
EVLE+ +P+ ++F+++ C +AA VYR+ QYD+ G +GSR A LN A
Sbjct: 7 EVLENMLKVSFPLSLVLFLVLVCPLRAEAAHEFSVYRMQQYDLQGQTYGSRNAILNTEAR 66
Query: 79 SLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKNDGTKC 138
++ LSR V++ L + + ++ + Q G ++ +LP ++ +
Sbjct: 67 TVE---AEVLSRRCVMMRLADFSYEKYQKALRQS--AGAVVIILP---------HNMSTL 126
Query: 139 PNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVK--------RNDATGQ 198
P + + ELE L+ PVYFA E E++ ++ + + A
Sbjct: 127 PQD----IVQQFMELEPELLATETIVPVYFALEDEELLSIYTQTQISSSSQGSSSAAEVL 186
Query: 199 LATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAA 258
L TAT G+++V S A+ + + IT+++G L G S G+ LPTI +VA YD+FG A
Sbjct: 187 LHTATANGFQMVTSGAQSKAVSDWAITSLEGRLTG--SGGE--DLPTIVLVAHYDSFGVA 246
Query: 259 PELSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ- 318
P LS G+DSNGSG+ LLE+ARLFS LYS +T YNLLF L+ GG +NY GT +WL+
Sbjct: 247 PWLSYGADSNGSGVAILLELARLFSRLYSYKRTHAGYNLLFFLSGGGKFNYQGTKRWLED 306
Query: 319 SFDHR----LRESIDYAICLNSIGSWDDKLWLHVSKPPEN-----VYIKQIFEDFSNVAE 378
+ DH L++++ + +CL+++G+ D L LHVSKPP+ +K++ ++
Sbjct: 307 NLDHTDASLLQDNVAFVLCLDTLGN-SDNLHLHVSKPPKEGSPQYTLLKELETVVAHQHP 366
Query: 379 DLGFKVDLKHKKINISNPRVAWEHEQFSRLRVTAATLSEL---------------SAAPE 438
DL F + HKKIN+++ +AWEHE+F R+ A TLS L S +P
Sbjct: 367 DLKFA--MVHKKINLADDTLAWEHERFGIRRLPAFTLSHLESHRSPARHSIMDMRSVSPS 426
Query: 439 LLERTGGLVDNRLFLNESAIAKSIKLVAESLARHIYRYEGK----NIQVFADDSSLAVNP 498
L G ++ ++++ K++AE+LAR IY K ++++F + + V
Sbjct: 427 L--EGAGEATTGPHVDLGKLSRNTKVIAETLARVIYNLTEKGVTGDLEIFTE--QMQVQE 486
Query: 499 TYIRSWLDLLSRTPRVAPFLSKDDPFILALKKELEVHTHDVS---MQHEAFDGMFTFYDS 546
+ S +D L+ PR A L KD I L+ +L + DV ++ + D F FYD
Sbjct: 487 DQLASLVDWLTAQPRAAQLLDKDSSIINTLEHQLSRYLKDVKRHLVRADKRDPEFVFYDQ 546
BLAST of MS002839 vs. ExPASy Swiss-Prot
Match:
Q969V3 (Nicalin OS=Homo sapiens OX=9606 GN=NCLN PE=1 SV=2)
HSP 1 Score: 247.7 bits (631), Expect = 3.2e-64
Identity = 171/543 (31.49%), Postives = 281/543 (51.75%), Query Frame = 0
Query: 29 ALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPPGADLSRTV 88
A++ ++ + DAA VYR+ QYD+ G P+G+R A LN A ++ LSR
Sbjct: 28 AVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTM---AAEVLSRRC 87
Query: 89 VIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKNDGTKCPNNGEGTVKNLLGE 148
V++ L + + ++ + Q G ++ +LP+ + + V+ + E
Sbjct: 88 VLMRLLDFSYEQYQKALRQS--AGAVVIILPRAMAAVP------------QDVVRQFM-E 147
Query: 149 LERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQ-----------LATATTGGYKL 208
+E ++ PVYFA E E A+L+ K+ A L TAT G+++
Sbjct: 148 IEPEMLAMETAVPVYFAVEDE---ALLSIYKQTQAASASQGSASAAEVLLRTATANGFQM 207
Query: 209 VVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPELSVGSDSNG 268
V S + + + I +++G L GL + LPTI IVA YD FG AP LS+G+DSNG
Sbjct: 208 VTSGVQSKAVSDWLIASVEGRLTGLGGE----DLPTIVIVAHYDAFGVAPWLSLGADSNG 267
Query: 269 SGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SFDHR----LR 328
SG+ LLE+ARLFS LY+ +T YNLLF + GG +NY GT +WL+ + DH L+
Sbjct: 268 SGVSVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDNLDHTDSSLLQ 327
Query: 329 ESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIF-EDFSNVA--EDLGFKVDLKHKKI 388
+++ + +CL+++G L LHVSKPP ++ F + VA + + + HK+I
Sbjct: 328 DNVAFVLCLDTVGR-GSSLHLHVSKPPREGTLQHAFLRELETVAAHQFPEVRFSMVHKRI 387
Query: 389 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 448
N++ +AWEHE+F+ R+ A TLS L + + + ++D R ++ + ++ +++A
Sbjct: 388 NLAEDVLAWEHERFAIRRLPAFTLSHLESHRD--GQRSSIMDVRSRVDSKTLTRNTRIIA 447
Query: 449 ESLARHIYRYEGK----NIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFIL 508
E+L R IY K ++ VF + + + + S +D L+ PR A + KD F+
Sbjct: 448 EALTRVIYNLTEKGTPPDMPVFTE--QMQIQQEQLDSVMDWLTNQPRAAQLVDKDSTFLS 507
Query: 509 ALKKELEVHTHDVSMQH---EAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLV 546
L+ L + DV H + D F FYD ++ Y+V FDLLL + + +YL
Sbjct: 508 TLEHHLSRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVGIAAYLG 540
BLAST of MS002839 vs. ExPASy Swiss-Prot
Match:
Q5ZJH2 (Nicalin OS=Gallus gallus OX=9031 GN=NCLN PE=2 SV=1)
HSP 1 Score: 221.9 bits (564), Expect = 1.9e-56
Identity = 167/541 (30.87%), Postives = 271/541 (50.09%), Query Frame = 0
Query: 23 SFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATLNHHAGSLHFPPGA 82
SF V V +L+ +AA VYR+ QY++ G P+G+R+A LN A ++ A
Sbjct: 21 SFLLFVPAVLLLLGPPPAAEAAHESTVYRMQQYELGGQPYGTRSAVLNTEARTVE----A 80
Query: 83 D-LSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKNDGTKCPNNGEGT 142
D LSR V++ L + + ++ + Q G ++ +LP+ S +
Sbjct: 81 DVLSRRCVMMRLVDFSYEQYQKALRQS--AGAVVIILPRSISSVP------------QDV 140
Query: 143 VKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATG--------QLATATTG 202
VK + +L E I PVYFA E +++ ++ + A+ L TAT
Sbjct: 141 VKQFMEIEPEMLAMETI-VPVYFAVEDDELLSIYEQTRAASASQGSASAAEVLLHTATAN 200
Query: 203 GYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPELSVGS 262
G+++V S A+ + + I +++G L GL + LPT+ IVA YD+FG AP LS G+
Sbjct: 201 GFQMVTSGAQSKAIHDWLIPSVEGRLTGLGGE----DLPTVVIVAHYDSFGVAPWLSHGA 260
Query: 263 DSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQ-SFDHR-- 322
DSNGSG+ LLE+ARLFS LY+ +T YNLLF + GG +NY GT +WL+ + DH
Sbjct: 261 DSNGSGVSVLLELARLFSRLYTYRRTHAGYNLLFFASGGGKFNYQGTKRWLEDNLDHTDS 320
Query: 323 --LRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGF---KVDLK 382
L++++ + +CL+++G + L LHVSKPP+ ++ F + F K +
Sbjct: 321 SLLQDNVAFVLCLDTLGR-GNSLHLHVSKPPKEGTLQHAFLRELEMVVASQFPEVKFSMV 380
Query: 383 HKKINISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSI 442
HKKIN++ +AWEHE+F+ R+ A T+S L + + L + ++D R ++ A+ +
Sbjct: 381 HKKINLAEDILAWEHERFAIRRLPAFTISHLESHRDSLRNS--IMDRRSQVDTKALTQEY 440
Query: 443 KLVAESLAR-HIYRYEGKNIQVFADDSSLAVNPTYIRSWLD-LLSRTPRVAPFLSKDDPF 502
+ H+ + AD +P D L ++ + A + KD F
Sbjct: 441 QDHCGGFDEGHLQPNREGSTCRSADLHGSDADPGGAARISDGLAEQSAQAAQLIDKDSTF 500
Query: 503 ILALKKELEVHTHDVSMQH---EAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSY 542
+ L+ + + DV H + D F FYD ++ Y+V FDLLL + + +Y
Sbjct: 501 LSTLEYYMGRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAIFDLLLAVCIAAY 535
BLAST of MS002839 vs. ExPASy TrEMBL
Match:
A0A6J1CME5 (Nicalin OS=Momordica charantia OX=3673 GN=LOC111012612 PE=3 SV=1)
HSP 1 Score: 1069.7 bits (2765), Expect = 4.2e-309
Identity = 541/544 (99.45%), Postives = 541/544 (99.45%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKK LGGLLFLLPKIFGSDDTKN
Sbjct: 61 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKXLGGLLFLLPKIFGSDDTKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT
Sbjct: 121 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL
Sbjct: 181 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISN RVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA
Sbjct: 361 NISNLRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSR PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRMPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV
Sbjct: 481 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. ExPASy TrEMBL
Match:
A0A6J1G0U3 (Nicalin OS=Cucurbita moschata OX=3662 GN=LOC111449666 PE=3 SV=1)
HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 505/544 (92.83%), Postives = 522/544 (95.96%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTV+I PLCELNITFVKECISQKKRLGGLLFL+PKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVIITPLCELNITFVKECISQKKRLGGLLFLVPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
R+ ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RIHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLVLLFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVLLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. ExPASy TrEMBL
Match:
A0A6J1HVN9 (nicalin-1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466578 PE=4 SV=1)
HSP 1 Score: 1008.1 bits (2605), Expect = 1.5e-290
Identity = 504/544 (92.65%), Postives = 521/544 (95.77%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAP KARER+VLESFYPV+ALVFILVACVE CDAATVVDVYRLIQYDISGVPFGSRAATL
Sbjct: 1 MAPPKARERQVLESFYPVLALVFILVACVEFCDAATVVDVYRLIQYDISGVPFGSRAATL 60
Query: 70 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 129
NHHAGSLHFP GADLSRTVVI PLCELNITFVKECISQKKRLGGLLFLLPKI G D KN
Sbjct: 61 NHHAGSLHFPSGADLSRTVVISPLCELNITFVKECISQKKRLGGLLFLLPKILGLDGLKN 120
Query: 130 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 189
+GT+CPNNGEG +K+LL +LER L+HENIPYPVYFASEG+DI AVLADVKRNDATGQLAT
Sbjct: 121 EGTRCPNNGEGMIKDLLAQLERFLIHENIPYPVYFASEGDDIDAVLADVKRNDATGQLAT 180
Query: 190 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 249
ATTGGYKLVVSA EPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAI+ASYDTFGAAP+L
Sbjct: 181 ATTGGYKLVVSAPEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIIASYDTFGAAPDL 240
Query: 250 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 309
SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS DH
Sbjct: 241 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSLDH 300
Query: 310 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 369
RL ESIDYAICLNSIGSWDDKLWLHVSKPPEN YIK+IFEDFSNVAEDLGFKVDLKHKKI
Sbjct: 301 RLHESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKKIFEDFSNVAEDLGFKVDLKHKKI 360
Query: 370 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 429
NISNPRVAWEHEQFSRLRVTAATLS LS APELLERTGGLVDNR FLNESA+AKSIKLVA
Sbjct: 361 NISNPRVAWEHEQFSRLRVTAATLSGLSTAPELLERTGGLVDNRSFLNESAVAKSIKLVA 420
Query: 430 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 489
ESLARHIYRY+GKNIQVFADDSSLAV+PTYIRSWLDLLSR+PRVAPFLSKDDPFILALKK
Sbjct: 421 ESLARHIYRYDGKNIQVFADDSSLAVHPTYIRSWLDLLSRSPRVAPFLSKDDPFILALKK 480
Query: 490 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 549
ELEVHT DVS+QHE FDGMFTFYDSTAAKLHIYQVASVTFDLLLLL LGSYLV LFCFLV
Sbjct: 481 ELEVHTSDVSLQHELFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLALGSYLVFLFCFLV 540
Query: 550 ITTR 554
ITTR
Sbjct: 541 ITTR 544
BLAST of MS002839 vs. ExPASy TrEMBL
Match:
A0A0A0LYB7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G167840 PE=4 SV=1)
HSP 1 Score: 1000.3 bits (2585), Expect = 3.1e-288
Identity = 504/550 (91.64%), Postives = 522/550 (94.91%), Query Frame = 0
Query: 4 APDLVSMAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFG 63
APDL SMAPRK RE +V +SFYPV+ALVFILVACVELCDAATVVDVYRLIQYDISGVPFG
Sbjct: 82 APDLASMAPRKPREPQVFDSFYPVLALVFILVACVELCDAATVVDVYRLIQYDISGVPFG 141
Query: 64 SRAATLNHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFG 123
SRAATLNHHA SLHFP GADLSRTV+IIPLCELN+TF++ECISQKKRLGGLL LLP+I G
Sbjct: 142 SRAATLNHHASSLHFPTGADLSRTVLIIPLCELNMTFLQECISQKKRLGGLLVLLPRILG 201
Query: 124 SDDTKNDGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDA 183
S+ KND KCP NGEG +K L ELERLLVH IPYPVYFASEGEDI AVLADVK NDA
Sbjct: 202 SESLKNDDIKCP-NGEGVIKGLSVELERLLVHSTIPYPVYFASEGEDIDAVLADVKNNDA 261
Query: 184 TGQLATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTF 243
TGQLATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTF
Sbjct: 262 TGQLATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTF 321
Query: 244 GAAPELSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKW 303
GAAP+LSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKW
Sbjct: 322 GAAPDLSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKW 381
Query: 304 LQSFDHRLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVD 363
LQSFDHRLRE IDYAICLNSIGSWDDKLWLHVSKPPEN YIKQIFEDFSNVAEDLGFKVD
Sbjct: 382 LQSFDHRLRERIDYAICLNSIGSWDDKLWLHVSKPPENAYIKQIFEDFSNVAEDLGFKVD 441
Query: 364 LKHKKINISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAK 423
LKHKKINISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGL DNRLFL+ES IAK
Sbjct: 442 LKHKKINISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLGDNRLFLDESKIAK 501
Query: 424 SIKLVAESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPF 483
SIKLVAESLARHIYRYEGKNIQVFADDSSLA+NPT+IRSWLDLLSRTPRVAPFLSKDDPF
Sbjct: 502 SIKLVAESLARHIYRYEGKNIQVFADDSSLAINPTFIRSWLDLLSRTPRVAPFLSKDDPF 561
Query: 484 ILALKKELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVL 543
I ALKKELEVHTHDVS+QHE F+G+FTFY STAAKLH+YQVASVTFDLLLLLVLGSYLVL
Sbjct: 562 ITALKKELEVHTHDVSLQHEVFEGIFTFYGSTAAKLHVYQVASVTFDLLLLLVLGSYLVL 621
Query: 544 LFCFLVITTR 554
LFCFLVITTR
Sbjct: 622 LFCFLVITTR 630
BLAST of MS002839 vs. ExPASy TrEMBL
Match:
A0A6J1IC52 (nicalin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471642 PE=4 SV=1)
HSP 1 Score: 988.8 bits (2555), Expect = 9.4e-285
Identity = 496/547 (90.68%), Postives = 516/547 (94.33%), Query Frame = 0
Query: 10 MAPRKAREREVLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 69
MAPRK RE +VLESFYP++ALVF+LVAC ELCDAA VVDVYRLI YDISGVPFGSRAA+L
Sbjct: 1 MAPRKPREPQVLESFYPLLALVFLLVACTELCDAAAVVDVYRLIHYDISGVPFGSRAASL 60
Query: 70 NHHAGSLHFPP---GADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDD 129
NHHA SLHFPP ADLSRTV IIPLCELN TFVKEC+SQ+KRLGGLL LLPKI GSD
Sbjct: 61 NHHAASLHFPPAAAAADLSRTVFIIPLCELNFTFVKECLSQRKRLGGLLILLPKILGSDG 120
Query: 130 TKNDGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQ 189
KND KCP NG+G +K+LL ELERLL+H +PYPVYFASEGEDI AVLADVK NDATGQ
Sbjct: 121 PKNDDFKCPQNGDGMIKDLLVELERLLIHATLPYPVYFASEGEDINAVLADVKSNDATGQ 180
Query: 190 LATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAA 249
LATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLK DGDASQLPTIAIVASYDTFGA+
Sbjct: 181 LATATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKFDGDASQLPTIAIVASYDTFGAS 240
Query: 250 PELSVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS 309
PELSVGSDSNGSGIVALLEIARLFSLLYS+PKTRGRYNLLFGLTSGGPYNYNGTHKWLQS
Sbjct: 241 PELSVGSDSNGSGIVALLEIARLFSLLYSSPKTRGRYNLLFGLTSGGPYNYNGTHKWLQS 300
Query: 310 FDHRLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKH 369
FDHR+RESIDYAICLNSIGSWDDKLWLHVSKPPEN YIKQIFEDFSNVAEDLGFKVDLKH
Sbjct: 301 FDHRIRESIDYAICLNSIGSWDDKLWLHVSKPPENAYIKQIFEDFSNVAEDLGFKVDLKH 360
Query: 370 KKINISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIK 429
KKINISNPRVAWEHEQFSRLRVTAATLS +SAAPELLERTGGL DNRLFLNESAIAKSIK
Sbjct: 361 KKINISNPRVAWEHEQFSRLRVTAATLSGISAAPELLERTGGLADNRLFLNESAIAKSIK 420
Query: 430 LVAESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILA 489
LVAESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFI A
Sbjct: 421 LVAESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFISA 480
Query: 490 LKKELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFC 549
LKKELEVHTHDVS+QHE FDG+FTFYDSTAAKLH+YQVASVTFDL+LLLVLGSYLVLLFC
Sbjct: 481 LKKELEVHTHDVSLQHEPFDGVFTFYDSTAAKLHVYQVASVTFDLVLLLVLGSYLVLLFC 540
Query: 550 FLVITTR 554
FLVITTR
Sbjct: 541 FLVITTR 547
BLAST of MS002839 vs. TAIR 10
Match:
AT3G44330.1 (INVOLVED IN: protein processing; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nicalin (InterPro:IPR016574), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Nicastrin (InterPro:IPR008710); Has 245 Blast hits to 243 proteins in 99 species: Archae - 6; Bacteria - 10; Metazoa - 139; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 44 (source: NCBI BLink). )
HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-230
Identity = 400/544 (73.53%), Postives = 466/544 (85.66%), Query Frame = 0
Query: 13 RKARERE---VLESFYPVVALVFILVACVELCDAATVVDVYRLIQYDISGVPFGSRAATL 72
+K+++R V ES YP++AL+ ILVACVELCDAATVVDVYRLIQYDISGVPFGSR ++L
Sbjct: 5 KKSKQRHRVMVFESMYPILALMLILVACVELCDAATVVDVYRLIQYDISGVPFGSRFSSL 64
Query: 73 NHHAGSLHFPPGADLSRTVVIIPLCELNITFVKECISQKKRLGGLLFLLPKIFGSDDTKN 132
NHHA SL F GADLSR+V+I+PL EL+I FV++ ISQK+ LGGLL LLP+ F +
Sbjct: 65 NHHAASLSFQRGADLSRSVLILPLRELDIAFVQDYISQKQSLGGLLILLPQTFRPGNVGG 124
Query: 133 DGTKCPNNGEGTVKNLLGELERLLVHENIPYPVYFASEGEDIGAVLADVKRNDATGQLAT 192
N+G ++LLG+LE+LLVH NIP+PVYFA E E+ A+LADVK+NDA GQ AT
Sbjct: 125 GSLSSENDG---FRSLLGQLEKLLVHGNIPFPVYFAFENEETDAMLADVKKNDALGQQAT 184
Query: 193 ATTGGYKLVVSAAEPRKLVSSTITNIQGWLPGLKSDGDASQLPTIAIVASYDTFGAAPEL 252
ATTGGYKLV+S +EPRK+ S TITNIQGWLPGL+++GD+SQLPTIA+VASYDTFGAAP L
Sbjct: 185 ATTGGYKLVISVSEPRKIASPTITNIQGWLPGLRAEGDSSQLPTIAVVASYDTFGAAPAL 244
Query: 253 SVGSDSNGSGIVALLEIARLFSLLYSNPKTRGRYNLLFGLTSGGPYNYNGTHKWLQSFDH 312
SVGSDSNGSG+VALLE+ARLFS+LYSNPKTRG+YNLLF LTSGGPYNY GT KWL+S D
Sbjct: 245 SVGSDSNGSGVVALLEVARLFSVLYSNPKTRGKYNLLFALTSGGPYNYEGTQKWLKSLDQ 304
Query: 313 RLRESIDYAICLNSIGSWDDKLWLHVSKPPENVYIKQIFEDFSNVAEDLGFKVDLKHKKI 372
R+RESIDYAICLNS+GSWD +L +HVSKPP+N YIKQIFE FSNVAEDLGF+V LKHKKI
Sbjct: 305 RMRESIDYAICLNSVGSWDSELLIHVSKPPDNAYIKQIFEGFSNVAEDLGFQVALKHKKI 364
Query: 373 NISNPRVAWEHEQFSRLRVTAATLSELSAAPELLERTGGLVDNRLFLNESAIAKSIKLVA 432
NISN RVAWEHEQFSRLRVTAATLSELS PELLE G L D R +NE AI K +KLVA
Sbjct: 365 NISNSRVAWEHEQFSRLRVTAATLSELSTPPELLENAGSLSDTRQLVNEDAIIKGVKLVA 424
Query: 433 ESLARHIYRYEGKNIQVFADDSSLAVNPTYIRSWLDLLSRTPRVAPFLSKDDPFILALKK 492
ESLA+HIY ++GK+I++FADDSSLAVNP Y+RSWLDLLS+TPRVAPFLSK++P I+ALKK
Sbjct: 425 ESLAKHIYGHQGKDIKIFADDSSLAVNPFYVRSWLDLLSQTPRVAPFLSKNEPLIMALKK 484
Query: 493 ELEVHTHDVSMQHEAFDGMFTFYDSTAAKLHIYQVASVTFDLLLLLVLGSYLVLLFCFLV 552
ELE +T +VS+QHE+ DG FTFYDST A L+IYQVASVTFDLLLLLVLGSYL++LF FLV
Sbjct: 485 ELEDYTAEVSVQHESLDGSFTFYDSTKASLNIYQVASVTFDLLLLLVLGSYLIVLFSFLV 544
Query: 553 ITTR 554
ITTR
Sbjct: 545 ITTR 545
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022142510.1 | 8.7e-309 | 99.45 | nicalin-1 [Momordica charantia] >XP_022142511.1 nicalin-1 [Momordica charantia] | [more] |
KAG7012715.1 | 7.3e-292 | 93.38 | Nicalin [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022945431.1 | 2.8e-291 | 92.83 | nicalin-1-like isoform X1 [Cucurbita moschata] | [more] |
KAG6573634.1 | 2.8e-291 | 93.20 | Nicalin, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_023542146.1 | 1.1e-290 | 93.01 | nicalin-1-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q5XIA1 | 1.4e-67 | 33.33 | Nicalin OS=Rattus norvegicus OX=10116 GN=Ncln PE=2 SV=1 | [more] |
Q8VCM8 | 3.1e-67 | 33.15 | Nicalin OS=Mus musculus OX=10090 GN=Ncln PE=1 SV=2 | [more] |
Q6NZ07 | 6.9e-67 | 32.92 | Nicalin-1 OS=Danio rerio OX=7955 GN=ncl1 PE=2 SV=1 | [more] |
Q969V3 | 3.2e-64 | 31.49 | Nicalin OS=Homo sapiens OX=9606 GN=NCLN PE=1 SV=2 | [more] |
Q5ZJH2 | 1.9e-56 | 30.87 | Nicalin OS=Gallus gallus OX=9031 GN=NCLN PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CME5 | 4.2e-309 | 99.45 | Nicalin OS=Momordica charantia OX=3673 GN=LOC111012612 PE=3 SV=1 | [more] |
A0A6J1G0U3 | 1.4e-291 | 92.83 | Nicalin OS=Cucurbita moschata OX=3662 GN=LOC111449666 PE=3 SV=1 | [more] |
A0A6J1HVN9 | 1.5e-290 | 92.65 | nicalin-1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466578 PE=4 SV=1 | [more] |
A0A0A0LYB7 | 3.1e-288 | 91.64 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G167840 PE=4 SV=1 | [more] |
A0A6J1IC52 | 9.4e-285 | 90.68 | nicalin-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471642 PE=4 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT3G44330.1 | 1.7e-230 | 73.53 | INVOLVED IN: protein processing; LOCATED IN: mitochondrion, endoplasmic reticulu... | [more] |