MS002807 (gene) Bitter gourd (TR) v1

Overview


Name	MS002807
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	heme-binding protein 2
Location	scaffold66: 784332 .. 786794 (-)
RNA-Seq Expression	MS002807
Synteny	MS002807

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGGTGAAGCTGTGGCTTCTATTGGCTCTCCTGATCGCTCCGAAGAAATCGGCCGGCGCCGAGTTTCCCCCAACCTGCAATCGTATCGAGTGCCCTAGCTTCGACGTCATCGAGACCGGCGATGGCTACGAGATTCGCCGCTACGATTCCGCGGTCTGGGCCTCCACCGCCCCCATTCAGGACATCTCTCTGCACGAGGCCACCAGACGAGGATTTCTGCAGTAAGAACTATCTTCATCTTCATCTTCCAAATTTCATTTCATGAATTTGTTCTACTGCGAATGCCATACTTGTAATTCTGATTGCATCTCTCATCTGTTCGGCTGGCTGTGGCTGGTGTGATAGATTAGATTTCTATAACTTCGAATAAGGCTATAGTAATTATTAAACCTTGCATTGCATCCGATTTGTTCTTGCTAACCTAATCCTAGTGTGAAACTAATTTTTCATTCAATCAGAGAATTGAAGAACACAGATTTGGTTAAATCAATCAAACTTAGGTGTTGTGGACTTTCTTGTACCTTTATTAATCTCCCAGACTCAATATAATCCTTGAAGCCTTTGATGCTACGGCTAACAAACTTACAAATTAAGCACACATTTGATTATTACTTGAAATGCAAACTTTTGTAACTCATTAAACTCTACGAGTTATGCCGCCAAGTTGAGCATATATCATATATTAAAAGATTGAAATCCCCACCTGATGTTTAACTAAAAAAAATTGGTTTAAAATACTATTTTGATCTTTGGTTTATTTTGGTCCAAATTACTATTTTGATCATTTTAAGTTTCGTTCATTTTGGTTTGAAAATGTTTAATTTAGTCCATTGTTTTCAATTTTAATTCATTTTAATTTCTATCATTTTAAAATGTTTGTATGGGTTCATATTTTAAAACAATGACTATTTGTCTTAATTTACAAAAAAAAAAAAAAAAAAAATAGCAAAAATTCTATGGGCCAAAATAGGTAACCTTTTAGAGTATAATGGGAACCAAATAAACAACTGGGCTTGAGTTTGGACTTTAATGAATTTAAACTTGGGACCTGGGCTTGGGTTTTGGAGTTTTAACAAAATGAATTTAAGCCTCTTGACTCTCTTTATGGAATTCCAAATGAACTTGCTTCTGGGCCTGGCTGGGGTTTTAGGTTTAGGGCTTTAACAAAGTGAAATTCAACTTCTACGTTTGGGCTTGGGCTACTGGTTTTAACATAACAACGAGTGATTTTGTACCCATTACTGGATAAATATTTACAGATATGAACCAAAGATATTACAAAACTAGTCTATCTTCTTCCAGCCTAGTTTACTATTTTCACCCTCATTTTTTGCTCCAAATTGTAAAGATGCATCATATAAAAGTTTAGTTCGGGATTGATGTCTACTGTGTTTTTAGGGGGAAAAAAATTCTGTTGTAATATTAAGTAATTAGTGTTTGGTAAATTTCAATTTTTAACCCAAAAAAAGTAGGTCACAGTGTTGAGGTTTTTCACAGAAGAGTAAATCATAAAGTGATTTATATAAATTAAAAAATAATCAAGTACCAAACATGTACGTTATACAGTTAAAATTCTCTCGTAATTACTTTTAACCCGGCCGGGTGTAACTTATTAGCTGTTTGCACTCGAAAAAACATATTAGCTAGGTGTTAAGTATAATTGTTCCTGAGTCCCCGAGGTATTGGGGCTTCAAGGAATATTTTTGAAGTCTTGGGTTTAAGTCACATAGAACGTAACTCCACCAAGCAATAACCTCTTGGGTATATACGAGTATGGGAACAGTGCGCATACCCCCGGGACGTCCATTAGGTAATTAAAAAAAAAAGAAAAAAGTATAATTGTTCCCGTTTACTTATGACTTTGATATTCACCTTACATTAAATGTATGATGCCTTCTTTTGTTTTCTTATTCTTTTTACACTTTATTTGACACATTACTTGCCATAAATGCTTAACATGTGTATTATACCTGCTACAATTGTTTGAGAAAAGATGGGATTTTTGTTGAACAACATTACATTACTTGTCCTAGATTATTCGATTACATTCAAGGAAAGAACAAGTACAATGAAAAGATAGAGATGACTGCTCCAGTGATTTCTGAGGTTTCTCCCAGTGATGGACCATTCTGTGCATCATCCTTTGTGGTTAGTTTCTATGTCCCCAAACTGAACCAAGCAGATCCACCTCCAGCCAAAGGGCTTCATGTTCAGCGATGGAAACCAACATATGTTGCAGTCAGACAGTTTAGCGGCTTCGTCGTCGATGCCAACATCGGAGCCGAGGCTGCTGCATTGGACGCCAGTCTCGTCGACACCAAGTGGGCTGCAGCCATCGCTAAGAGTCGCCGCGTCGAACATGCTTCAATTTATACTGTGGCTCAGTACAATTCTCCTTTTGAGTTCGACCATCGGGTGAATGAGATATGGTTCTTGTTTGACATTGAAGACATTGCT

mRNA sequence

ATGGAAATGGTGAAGCTGTGGCTTCTATTGGCTCTCCTGATCGCTCCGAAGAAATCGGCCGGCGCCGAGTTTCCCCCAACCTGCAATCGTATCGAGTGCCCTAGCTTCGACGTCATCGAGACCGGCGATGGCTACGAGATTCGCCGCTACGATTCCGCGGTCTGGGCCTCCACCGCCCCCATTCAGGACATCTCTCTGCACGAGGCCACCAGACGAGGATTTCTGCAATTATTCGATTACATTCAAGGAAAGAACAAGTACAATGAAAAGATAGAGATGACTGCTCCAGTGATTTCTGAGGTTTCTCCCAGTGATGGACCATTCTGTGCATCATCCTTTGTGGTTAGTTTCTATGTCCCCAAACTGAACCAAGCAGATCCACCTCCAGCCAAAGGGCTTCATGTTCAGCGATGGAAACCAACATATGTTGCAGTCAGACAGTTTAGCGGCTTCGTCGTCGATGCCAACATCGGAGCCGAGGCTGCTGCATTGGACGCCAGTCTCGTCGACACCAAGTGGGCTGCAGCCATCGCTAAGAGTCGCCGCGTCGAACATGCTTCAATTTATACTGTGGCTCAGTACAATTCTCCTTTTGAGTTCGACCATCGGGTGAATGAGATATGGTTCTTGTTTGACATTGAAGACATTGCT

Coding sequence (CDS)

ATGGAAATGGTGAAGCTGTGGCTTCTATTGGCTCTCCTGATCGCTCCGAAGAAATCGGCCGGCGCCGAGTTTCCCCCAACCTGCAATCGTATCGAGTGCCCTAGCTTCGACGTCATCGAGACCGGCGATGGCTACGAGATTCGCCGCTACGATTCCGCGGTCTGGGCCTCCACCGCCCCCATTCAGGACATCTCTCTGCACGAGGCCACCAGACGAGGATTTCTGCAATTATTCGATTACATTCAAGGAAAGAACAAGTACAATGAAAAGATAGAGATGACTGCTCCAGTGATTTCTGAGGTTTCTCCCAGTGATGGACCATTCTGTGCATCATCCTTTGTGGTTAGTTTCTATGTCCCCAAACTGAACCAAGCAGATCCACCTCCAGCCAAAGGGCTTCATGTTCAGCGATGGAAACCAACATATGTTGCAGTCAGACAGTTTAGCGGCTTCGTCGTCGATGCCAACATCGGAGCCGAGGCTGCTGCATTGGACGCCAGTCTCGTCGACACCAAGTGGGCTGCAGCCATCGCTAAGAGTCGCCGCGTCGAACATGCTTCAATTTATACTGTGGCTCAGTACAATTCTCCTTTTGAGTTCGACCATCGGGTGAATGAGATATGGTTCTTGTTTGACATTGAAGACATTGCT

Protein sequence

MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAPIQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVPKLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA

Homology

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BLAST of MS002807 vs. NCBI nr
Match: XP_022142929.1 (heme-binding protein 2 [Momordica charantia])

HSP 1 Score: 443.0 bits (1138), Expect = 1.5e-120
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP
Sbjct: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP
Sbjct: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS
Sbjct: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA
Sbjct: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 217

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BLAST of MS002807 vs. NCBI nr
Match: XP_008451794.1 (PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding protein 2 [Cucumis melo var. makuwa] >TYK16344.1 heme-binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 355.5 bits (911), Expect = 3.2e-94
Identity = 171/217 (78.80%), Postives = 190/217 (87.56%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEM +LW +++L++     A +EFPPTC RIECPS+DVIETGDG+EIRRY+S VWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISLHEATR GFLQLFDYIQGKNKYNEKIEMT PVI+EVSPSDGPFC SSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRW  TYVAVRQF GFV DANIG+EAAALD S+ DTKW AAI+KS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R     S+YTVAQYNSPFEF+ RVNEIWFLFDIED+A
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVA 216

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BLAST of MS002807 vs. NCBI nr
Match: XP_004137252.1 (heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_015165 [Cucumis sativus])

HSP 1 Score: 352.1 bits (902), Expect = 3.5e-93
Identity = 167/217 (76.96%), Postives = 188/217 (86.64%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEM KLW++++L++     A +EFPPTC RIECPS+DVI TGDG+EIRRY+S VWASTAP
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISL EATR GFLQLFDYIQGKN +NEKIEMT PVI+E+SPSDGPFC SSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRW  TYVAVRQF GFV DANIG+EA+ALD S+ DTKW AAI+KS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R     SIYTVAQYNSPFEF+ RVNEIWFLFDIED+A
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVA 217

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BLAST of MS002807 vs. NCBI nr
Match: XP_022984380.1 (heme-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 350.1 bits (897), Expect = 1.3e-92
Identity = 169/217 (77.88%), Postives = 191/217 (88.02%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           +EM+KL LL  L++   + A +E PP+C RIECPS+DVI++G G+EIRRYDS+VWASTAP
Sbjct: 6   IEMLKLSLLTTLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSSVWASTAP 65

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISLHEATR GFL+LFDYIQGKNKYNEKIEMTAPV++EVSPSDGPFC SSFVVSFYVP
Sbjct: 66  IPDISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVP 125

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRWK T+VAVRQF GFV DA IGAEAAALD S+ DTKWAAAI+KS
Sbjct: 126 KVNQADPPPAKGLHIQRWKETHVAVRQFGGFVSDATIGAEAAALDESVFDTKWAAAISKS 185

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R    A+IYTVAQYNSPFEF+ RVNEIWFLFDIED A
Sbjct: 186 RGAGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAA 222

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BLAST of MS002807 vs. NCBI nr
Match: XP_023552168.1 (heme-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 347.8 bits (891), Expect = 6.7e-92
Identity = 169/215 (78.60%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 3   MVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAPIQ 62
           +VKL LL  L++   + A +E PP+C RIECPS+DVI++G G+EIRRYDS VWASTAPI 
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 63  DISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVPKL 122
           DISLHEATR GFL+LFDYIQGKNKYNEKIEMTAPVI+EVSPSDGPFC SSFVVSFYVPK+
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVITEVSPSDGPFCESSFVVSFYVPKV 125

Query: 123 NQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRR 182
           NQADPPPAKGLH+QRWK TYVAVRQF GFV DA IGAEAAALD S+ DTKW AAI+KSR 
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 183 VEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
              A+IYTVAQYNSPFEF+ RVNEIWFLFDIED A
Sbjct: 186 AGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAA 220

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BLAST of MS002807 vs. ExPASy Swiss-Prot
Match: Q9Y5Z4 (Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-20
Identity = 65/196 (33.16%), Postives = 92/196 (46.94%), Query Frame = 0


Query: 21  GAEFPPTCNRIECPSFDVIETG----DGYEIRRYDSAVWASTAPIQDISLHEATRRGFLQ 80
           GA        +E P +   E        YEIR Y  A W ST+ ++ +    A + GF +
Sbjct: 10  GAAEDAAAQAVETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTS-VESMDWDSAIQTGFTK 69

Query: 81  LFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVPKLNQADPPP--AKGLH 140
           L  YIQGKN+   KI+MTAPV S V P  GPF  S+  +S Y+P   Q DPP      + 
Sbjct: 70  LNSYIQGKNEKEMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVF 129

Query: 141 VQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRRVEHASIYTVAQY 200
           ++      V VR F GF       + A      L+    A+ + +  +V    +Y  A Y
Sbjct: 130 IEDRAEMTVFVRSFDGF-------SSAQKNQEQLLTL--ASILREDGKVFDEKVYYTAGY 189

Query: 201 NSPFEFDHRVNEIWFL 211
           NSP +  +R NE+W +
Sbjct: 190 NSPVKLLNRNNEVWLI 195

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BLAST of MS002807 vs. ExPASy Swiss-Prot
Match: Q9WU63 (Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-19
Identity = 62/186 (33.33%), Postives = 91/186 (48.92%), Query Frame = 0


Query: 31  IECPSFDVIETGD----GYEIRRYDSAVWASTAPIQDISLHEATRRGFLQLFDYIQGKNK 90
           +E PS+   E  D     YEIR Y  A W ST  ++ +    A + GF +L  YIQGKN+
Sbjct: 20  LEMPSWKAPEDIDPQPGSYEIRHYGPAKWVSTC-VESLDWDSAIQTGFTKLNGYIQGKNE 79

Query: 91  YNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVPKLNQADPP-PAKG-LHVQRWKPTYVA 150
              KI++TAPV S V P   PF  S+  +S Y+P   Q DPP P++  + ++      V 
Sbjct: 80  KEMKIKLTAPVTSYVEPGSSPFSESTITISLYIPSEQQPDPPRPSESDVFIEDRAEMTVF 139

Query: 151 VRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRRVEHASIYTVAQYNSPFEFDHRV 210
           VR F GF        +   L         A  + +  +V +  ++  A Y+SPF+   R 
Sbjct: 140 VRSFDGFSSGQKNQEQLLTL---------ANILREEGKVFNEKVFYTAGYSSPFQLLDRN 195

[Close]

BLAST of MS002807 vs. ExPASy Swiss-Prot
Match: Q9SR77 (Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g10130 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-07
Identity = 57/194 (29.38%), Postives = 82/194 (42.27%), Query Frame = 0


Query: 31  IECPSFDVIETGDGYEIRRYDSAVWASTAPIQDISLHE-ATRRGFLQLFDYIQGKNKYNE 90
           +E  +F V+   D YEIR+ +    A T    +         + F  L +Y+ GKN   E
Sbjct: 114 LETMNFRVLFRTDKYEIRQVEPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIKE 173

Query: 91  KIEMTAPVISEVSPSDG-------PFCAS------SFVVSFYVPKLNQADPPPAK--GLH 150
           K+EMT PV++    S G       P   S       + +SF +P    ++ P  K   + 
Sbjct: 174 KMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSVK 233

Query: 151 VQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRRVEHASIYTVAQY 208
           +Q+     VAV  FSG+V D  I      L  +L +        K  RV     + VAQY
Sbjct: 234 IQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQND-------KKFRVRDGVSFEVAQY 293

[Close]

BLAST of MS002807 vs. ExPASy TrEMBL
Match: A0A6J1CMU7 (heme-binding protein 2 OS=Momordica charantia OX=3673 GN=LOC111012922 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.4e-121
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP
Sbjct: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP
Sbjct: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS
Sbjct: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA
Sbjct: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 217

[Close]

BLAST of MS002807 vs. ExPASy TrEMBL
Match: A0A5A7V4M9 (Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001260 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 171/217 (78.80%), Postives = 190/217 (87.56%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEM +LW +++L++     A +EFPPTC RIECPS+DVIETGDG+EIRRY+S VWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISLHEATR GFLQLFDYIQGKNKYNEKIEMT PVI+EVSPSDGPFC SSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRW  TYVAVRQF GFV DANIG+EAAALD S+ DTKW AAI+KS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R     S+YTVAQYNSPFEF+ RVNEIWFLFDIED+A
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVA 216

[Close]

BLAST of MS002807 vs. ExPASy TrEMBL
Match: A0A1S3BSD4 (heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 171/217 (78.80%), Postives = 190/217 (87.56%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEM +LW +++L++     A +EFPPTC RIECPS+DVIETGDG+EIRRY+S VWASTAP
Sbjct: 1   MEMGRLW-VVSLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISLHEATR GFLQLFDYIQGKNKYNEKIEMT PVI+EVSPSDGPFC SSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRW  TYVAVRQF GFV DANIG+EAAALD S+ DTKW AAI+KS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R     S+YTVAQYNSPFEF+ RVNEIWFLFDIED+A
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVA 216

[Close]

BLAST of MS002807 vs. ExPASy TrEMBL
Match: A0A0A0KVD6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 167/217 (76.96%), Postives = 188/217 (86.64%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           MEM KLW++++L++     A +EFPPTC RIECPS+DVI TGDG+EIRRY+S VWASTAP
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISL EATR GFLQLFDYIQGKN +NEKIEMT PVI+E+SPSDGPFC SSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRW  TYVAVRQF GFV DANIG+EA+ALD S+ DTKW AAI+KS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R     SIYTVAQYNSPFEF+ RVNEIWFLFDIED+A
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVA 217

[Close]

BLAST of MS002807 vs. ExPASy TrEMBL
Match: A0A6J1J8G7 (heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 6.5e-93
Identity = 169/217 (77.88%), Postives = 191/217 (88.02%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDSAVWASTAP 60
           +EM+KL LL  L++   + A +E PP+C RIECPS+DVI++G G+EIRRYDS+VWASTAP
Sbjct: 6   IEMLKLSLLTTLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSSVWASTAP 65

Query: 61  IQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVSFYVP 120
           I DISLHEATR GFL+LFDYIQGKNKYNEKIEMTAPV++EVSPSDGPFC SSFVVSFYVP
Sbjct: 66  IPDISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVP 125

Query: 121 KLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKS 180
           K+NQADPPPAKGLH+QRWK T+VAVRQF GFV DA IGAEAAALD S+ DTKWAAAI+KS
Sbjct: 126 KVNQADPPPAKGLHIQRWKETHVAVRQFGGFVSDATIGAEAAALDESVFDTKWAAAISKS 185

Query: 181 RRVEHASIYTVAQYNSPFEFDHRVNEIWFLFDIEDIA 218
           R    A+IYTVAQYNSPFEF+ RVNEIWFLFDIED A
Sbjct: 186 RGAGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAA 222

[Close]

BLAST of MS002807 vs. TAIR 10
Match: AT1G17100.1 (SOUL heme-binding family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-74
Identity = 139/223 (62.33%), Postives = 168/223 (75.34%), Query Frame = 0


Query: 5   KLWLLLAL------LIAPKKSAG----AEFPPTCNRIECPSFDVIETGDGYEIRRYDSAV 64
           KL  LL+L      L+ P   +G     +FPP+CNRIECPS++++ +G+GYEIRRY++ V
Sbjct: 9   KLSFLLSLLSGGSDLLGPDAESGVAQIGKFPPSCNRIECPSYELVHSGNGYEIRRYNNTV 68

Query: 65  WASTAPIQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFV 124
           W ST PI DISL +ATR  F QLF YIQGKN+Y++KIEMTAPVIS+VSPSDGPFC SSF 
Sbjct: 69  WVSTEPIPDISLVDATRTAFFQLFAYIQGKNEYHQKIEMTAPVISQVSPSDGPFCESSFT 128

Query: 125 VSFYVPKLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWA 184
           VSFYVPK NQ DP P++ LH+Q+W   YVAVRQFSGFV D +IG +AAALD+SL  T WA
Sbjct: 129 VSFYVPKKNQPDPAPSENLHIQKWNSRYVAVRQFSGFVSDDSIGEQAAALDSSLKGTAWA 188

Query: 185 AAIAKSRR---VEHASIYTVAQYNSPFEFDHRVNEIWFLFDIE 215
            AIAKS+    V   S YTVAQYNSPFEF  RVNEIW  F+++
Sbjct: 189 NAIAKSKEDGGVGSDSAYTVAQYNSPFEFSGRVNEIWLPFELD 231

[Close]

BLAST of MS002807 vs. TAIR 10
Match: AT1G78450.1 (SOUL heme-binding family protein )

HSP 1 Score: 216.9 bits (551), Expect = 1.7e-56
Identity = 114/222 (51.35%), Postives = 147/222 (66.22%), Query Frame = 0


Query: 1   MEMVKLWLLLALLIAPKKSAGAE----FPPTCNRIECPSFDVIETGDGYEIRRYDSAVWA 60
           + + KL  LL LL     S G++     PP CNR ECPS++V+  G+GYEI RY++ VW 
Sbjct: 5   LSIFKLSFLLTLL-----SGGSDLIDSLPPNCNRAECPSYEVVHAGNGYEIHRYNTTVWI 64

Query: 61  STAPIQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASSFVVS 120
           ST PIQDISL+EA+  G+ QL DY+ G N Y+++IE+  P I++VS +      S+F+VS
Sbjct: 65  STEPIQDISLNEASGNGWNQLSDYMNGNNDYHQRIEIALPYITQVSQN-----LSTFIVS 124

Query: 121 FYVPKLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAA 180
           F+VPK  Q DPPP   LHVQRW   YVAV+Q SG+V D  IG + A L ASL  T WA A
Sbjct: 125 FFVPKAFQPDPPPGNNLHVQRWDSRYVAVKQISGYVADHKIGKQVAELKASLQGTVWAKA 184

Query: 181 IAKSRR---VEHASIYTVAQYNSPFEFDHRVNEIWFLFDIED 216
           I KSR    V  A  YTVAQ++ PF++  RVNEIWF F++ED
Sbjct: 185 IEKSRETGGVGSAWAYTVAQFSWPFQWSQRVNEIWFPFEMED 216

[Close]

BLAST of MS002807 vs. TAIR 10
Match: AT1G78460.1 (SOUL heme-binding family protein )

HSP 1 Score: 161.4 bits (407), Expect = 8.2e-40
Identity = 95/222 (42.79%), Postives = 133/222 (59.91%), Query Frame = 0


Query: 1   MEMVKLWLLLALLI--------APKKSAGAEFPPTCNRIECPSFDVIETGDGYEIRRYDS 60
           + ++KL L ++L++        AP   +    P TC+  ECP++ ++E G G+EIR YD+
Sbjct: 5   LNILKLSLCVSLVVVGSYAQAPAPWNPSNGFRPGTCDHYECPTYKLVEAGYGFEIRMYDA 64

Query: 61  AVWASTAPIQDISLHEATRRGFLQLFDYIQGKNKYNEKIEMTAPVISEVSPSDGPFCASS 120
           A+W ST+PI  +S+ +AT+ GF +L  YI+G NK N K+ MTAPVI++ +P       S 
Sbjct: 65  ALWISTSPIPSLSMTQATKTGFRRLNRYIEGDNKSNVKMNMTAPVIAQATPG-----RSV 124

Query: 121 FVVSFYVPKLNQADPPPAKGLHVQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTK 180
           + VS Y+PK NQ +PP A  LHV+  KPTYVAVRQ  G+V +     EAAAL  SL D+ 
Sbjct: 125 YTVSLYLPKKNQQNPPQADDLHVRSTKPTYVAVRQIGGYVSNNVAKDEAAALMESLRDSN 184

Query: 181 WAAAIAKSRRVEHASIYTVAQYNSPFEFDHRV-NEIWFLFDI 214
           W   I KS+    A  Y +A YN P     RV NEI   F++
Sbjct: 185 WILPIEKSKGKLPA--YFLAVYNPPSHTTARVINEIMVPFNM 219

[Close]

BLAST of MS002807 vs. TAIR 10
Match: AT3G10130.1 (SOUL heme-binding family protein )

HSP 1 Score: 58.5 bits (140), Expect = 7.5e-09
Identity = 57/194 (29.38%), Postives = 82/194 (42.27%), Query Frame = 0


Query: 31  IECPSFDVIETGDGYEIRRYDSAVWASTAPIQDISLHE-ATRRGFLQLFDYIQGKNKYNE 90
           +E  +F V+   D YEIR+ +    A T    +         + F  L +Y+ GKN   E
Sbjct: 114 LETMNFRVLFRTDKYEIRQVEPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIKE 173

Query: 91  KIEMTAPVISEVSPSDG-------PFCAS------SFVVSFYVPKLNQADPPPAK--GLH 150
           K+EMT PV++    S G       P   S       + +SF +P    ++ P  K   + 
Sbjct: 174 KMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSVK 233

Query: 151 VQRWKPTYVAVRQFSGFVVDANIGAEAAALDASLVDTKWAAAIAKSRRVEHASIYTVAQY 208
           +Q+     VAV  FSG+V D  I      L  +L +        K  RV     + VAQY
Sbjct: 234 IQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQND-------KKFRVRDGVSFEVAQY 293

[Close]

BLAST of MS002807 vs. TAIR 10
Match: AT5G20140.1 (SOUL heme-binding family protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.0e-05
Identity = 34/141 (24.11%), Postives = 66/141 (46.81%), Query Frame = 0


Query: 31  IECPSFDVIETGDGYEIRRYDSAVWASTAPIQDISLHEATRRGFLQLFDYIQGKNKYNEK 90
           +E P + +++    YE+R Y+  +      ++ I    +   GF  +  YI GKN   EK
Sbjct: 205 LETPKYQILKRTANYEVRNYEPFI-----VVETIGDKLSGSSGFNNVAGYIFGKNSTMEK 264

Query: 91  IEMTAPVISEVSPSDGPFCASSFVVSFYVPK---LNQADPPPAKGLHVQRWKPTYVAVRQ 150
           I MT PV ++ + +     +S   V   +P    L+    P  + +++++ +  + A  +
Sbjct: 265 IPMTTPVFTQTTDTQ---LSSDVSVQIVIPSGKDLSSLPMPNEEKVNLKKLEGGFAAAVK 324

Query: 151 FSGFVVDANIGAEAAALDASL 169
           FSG   +  + A+   L +SL
Sbjct: 325 FSGKPTEDVVQAKENELRSSL 337

The following BLAST results are available for this feature:

BLAST of MS002807 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142929.1	1.5e-120	100.00	heme-binding protein 2 [Momordica charantia]	[more]
XP_008451794.1	3.2e-94	78.80	PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding prot...	[more]
XP_004137252.1	3.5e-93	76.96	heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_01...	[more]
XP_022984380.1	1.3e-92	77.88	heme-binding protein 2-like [Cucurbita maxima]	[more]
XP_023552168.1	6.7e-92	78.60	heme-binding protein 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS002807 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y5Z4	3.2e-20	33.16	Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1	[more]
Q9WU63	2.7e-19	33.33	Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1	[more]
Q9SR77	1.1e-07	29.38	Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]

BLAST of MS002807 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMU7	7.4e-121	100.00	heme-binding protein 2 OS=Momordica charantia OX=3673 GN=LOC111012922 PE=3 SV=1	[more]
A0A5A7V4M9	1.5e-94	78.80	Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A1S3BSD4	1.5e-94	78.80	heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1	[more]
A0A0A0KVD6	1.7e-93	76.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1	[more]
A0A6J1J8G7	6.5e-93	77.88	heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=...	[more]

BLAST of MS002807 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17100.1	1.3e-74	62.33	SOUL heme-binding family protein	[more]
AT1G78450.1	1.7e-56	51.35	SOUL heme-binding family protein	[more]
AT1G78460.1	8.2e-40	42.79	SOUL heme-binding family protein	[more]
AT3G10130.1	7.5e-09	29.38	SOUL heme-binding family protein	[more]
AT5G20140.1	1.0e-05	24.11	SOUL heme-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011256	Regulatory factor, effector binding domain superfamily	GENE3D	3.20.80.10	Regulatory factor, effector binding domain	coord: 30..215 e-value: 1.1E-52 score: 180.4
IPR011256	Regulatory factor, effector binding domain superfamily	SUPERFAMILY	55136	Probable bacterial effector-binding domain	coord: 24..211
IPR006917	SOUL haem-binding protein	PFAM	PF04832	SOUL	coord: 31..209 e-value: 3.7E-47 score: 160.7
IPR006917	SOUL haem-binding protein	PANTHER	PTHR11220	HEME-BINDING PROTEIN-RELATED	coord: 12..215
None	No IPR available	PANTHER	PTHR11220:SF25	F3F9.4	coord: 12..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002807.1	MS002807.1	mRNA