MS002750 (gene) Bitter gourd (TR) v1

Overview
NameMS002750
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionreticulon-4-interacting protein 1, mitochondrial
Locationscaffold66: 260338 .. 265946 (-)
RNA-Seq ExpressionMS002750
SyntenyMS002750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGAGCTCTACTGCGATGTCGCACCTCTGAATTCGAGCACGGATCGCCTTTTCTTGCTGGCTTTTGGTTTAGAACCATCGTCACCAGTTGCAGAGCCGTGGTTTTGCCCCGCTTCGGCGGTCCGGAGGTGCTCCAGCTCCGTCCCGACGTTGACGTCCCCAATCTCAAGCCTAATGAGGTTCTCGTCCGTGCTCGTGCTGTTTCCATCAACCCGCTTGATACCAGAGTCAGATTACCTTCTTTTGTGTCTCTTCCGGTTTTGGTTTTGCTTTGTCTGTGATTGCCTGAGCTTGGAAATGAATGTTTCCAAGAAAATTGAAATGGGTGTTGCTAGATTTCACCGTTCTTGGTAATTTATAGCATATGACTTGAGGGGGACTGTTTTTTACTTTTTCTTTATTCGGTCGGTTTTTACTGCGTTTGCTTGGAATCTTGAGTGGCTATTTGGTACTGAAACTAAATGAAATCATAACTTTTGGGGTCTTAATGTTTCCCAGATGCGATCAGGTTATGGTCGCTCAATATTTGAACCACTTCTACCGGTCATTTTGGGCCGTGATATCAGTGGTGAAGTTGCGGCTGTTGGTTCTTCAGTTCGTTCAGTAAGTGTTGGAGAAGAAGTTTTTGGTGCTCTGCATCCAACTGCTGTGAGGGGTACTTACGCTGATTATGCAATACTTTCAGACGATGAATTAAGTGCTAAACCAGTGTCATTTACACATGTGGTAATCATTGAAGTGCCTCCTTGTGATTTCTCTTGAATATTCATTTACTGTTTCTGTTTGGTCCTCTTGGTGTTGTGTATTTAATTTTTCTAATCCATGGTCTAACGTTCTTTCTTGTTTAGCCTAATCTATTTATTCATCCTGCAAACTTTTGTGTGGCTGTAAGTTTCTACGTGGTTATGCTGTGTTTCTTCATTAGCTTTTATTTGGGGAAATTCAGATGTAATTCTCACTCAAGACTTGAAAGTTTTTGTACCAATCAAAGTGGAGGAAAATGTTAGGAACATTATTGCGGGCATATTAGTAATTAACTTGAGAGTTTGTTAGAGTGGATTTAGTTATATGTAGAGTTAGTAGAGTTAGTTAGGGAGTTCGAGATTAGGAAAATTTTGAGTAATGAATAGTGGGAGTCGCATGCCTTTGTATGTTTGCCCAATCTGTTAGGAACACACATAGCATGTCTCCATAATTTTTCCTTTCAAATTCTGGTTCCAATTAACTTTTATTTGGAGCGACAAATCTTTGCCAATTGAGAGTCCTTTTTCTGAATCTTTGGCTTGCAAGAATATCTCATCTCCCCTTTTTTGTAATGTCATTTTTTGATAATGAAAGCATCGTTTCTTAAAAAAAAAAACACTTATTTTTCTTTTGGCGGAGGTGAACCTTTCTGGTGGTATCTGGAGGTTTTTTATTCACTTATTGATATTGAGCATAGTATCATGAAGCTTTTCTGCAATCCTCGTCATCAAGACTTTTAAGCCTTTGTTGGTTGACACCCATTGGACTTAGGTACATTATTCTGCCCAAATTATGCAACCTACCATCTAAATCTCTAAGACCATTCATAGAAATTTTATTTCGCTCAAAAGGTGTTCTAGTGCATTAGACTTGTATCAAATGCCATGTGAATTTCCAGAAACATGTGAACTGTATAGCTGACCCAATAATTGAAGTTTCATAAGAAAAGTAGAGCAGGCTGCCATGAGACAAATAGAAGATCTACTTCCTAGTGAAATTTGGTTTAGAACTATCATATGTCTAAGGTTCAATATTGAAATAATTTTAGAGGTTTTCCTTGACTTTGTCCTTGTTAACAAGCAATCTGTCCTATTTGTAGAGAATATAAACTCTCTAATGAAATAAGCTTGTCTAACTAAAAAACAAGACTAGTAAACTTCTTACCTAACAATCTAACTATTACTGCAACCAATTAACAAGCTAATCCAACAAACAACAGACTTAAACAAAAAATTACACGGTTATCTTTATTCTACGACTGTCATTTTCAGGTATAATCAAGGAGAGAAAGGTCATTTCTTTTTGAAAGCATTAGGGGGAATGAAGGGGTTGAGGTGTCAAGCATTTACTGTCCCTTGGATTATTTGTGATAAGGTTTATTGAACGACAAAATCTCCTTCCCAAATTGACTGAGAATATTACAGCAATTGGAGAAAAAATTAATCACTTGTTAACCAACTTCTTAACAAGTATTTCAACTAAAAATTGACTTCAAATTCTAACATTCATCGGGACAGGGTTATTGTTAAGAGAGACGCCACTGCATATAAGTAATCGTTAAATATCCTTATTAAGTGAAATAAATTGGTGTTAGATTGTTGGTTGGTTAGATTTTTGATGTGTCATGTAAACCTGGTAGTACTCTCATTCTACAACTAGATTATATTTAGTTAATCGGTTTTTCATTTCACATAGCTTTTTATCCTTATACTTCAATGAGAGAGAATTCGTTCATGAAAGATAGGACAAGCAGTCCTACCTAATAAAAAAAGAATATACCCTGCCATTTTACTTCATTTTGCAATTTTTTTGTTCTATCAGGAGGCAAGTGCCATTCCTTTTGCTGCACTGACTGCATGGCGGGCTCTAAAAAGTACTGCTAGAATCACCCAGGGGTATGTTTACTTTGTTCTATTAGTTTCTACGTTTTGTTCCAACTGAATATATTAATCATCATATAATATCTATGATAATTGTATTTCATTTTTGGGATCTAGTCAAAATCTTTGCTTTTTCTTAGTGCTCTCTTTCAAATTTGTTTTGTAATTACATTTTTCTCAAATTTATGCCGACCGGGAAGCCTTTTTGTAATCCTCTTGGCTTGATGGGGATAGCTCATCTCCATCCTTTTGTACCTCTTTTGATAATGAAATGTTCAGTTTCTTATAAAGAGAAGAAAAAAAAAGATAATTGTATTTCGTTTTTGAATTACACTTTCTGTGATTTCACTGTTCTGTATTGAAAATATATTAAATAATCTTATTTGTAGTTTGTATTCTTTTGATAGGATTCTTTTTGGTGATTTTCCTTAAAATCTAATCTTCTTTTGTGGCAGGCATAGGGTATTAGTAATTGGTGGTGGAGGAGCCGTAGGTTCTGCTGCAGTTCAGATTTCAGTTGCTTCAGGCTGCCACGTCACAACTACTTGTGGAAGTCAAAGTATAGATAAATTACTTGCAGCAGGTGCTGAGCAAGCAGTTGACTATACTACCGAGGTGATGGTTCATACAGTAGCCAGTCTGTTCGTCACCATATTTATTTACTATCTTGCCAATGTTATTGGATTGCCATGGATGGTTTGCATTTGGAAGCTGGGAGCTTGAATAATTTTCTTTTTTGGCTCGACATGAATATTTATAGGACATTGAATTGGTAATAAAGGGGAAATTTGATGCTGTTTTGGATACCATTGGTGCGCCAGATACTGAACGAATTGGCATCAGTCTTTTGAAGAGAGGTGGACACTATATGACACTTCAGGTATAGAGCTAACCAATCTTTCTCGAAGTAATTGAAGTGCTCTTCACATTCATGCTTTTAATTCCCATTTTTTCAGGGTGAGGCTGCATCATTCACTGATAAATATGGGCTACTTTTCGGGCTCCCTATAGCTACAAGTGTATTGGCGAAGAAACAGATACAGTACAGATACTCTCATGGAATCGGTAATAATCAAAATTTGTGTACTTCACTTACATTTTCAACATTGTGTTCCCTCCTGCTTGAAATTTTCCCTATCAATTTATATGTGAATAAGGATGGTCAGAATGCATTAATATTTTAAAGGACCTCGGTCTCCTAGTTTCTTGTGCTTAAGCTTGCCTTTTTTTTCTCCTCTTTTTAATTGTATTCTTGAGCTTAAGTTGTTATAAGAGCAAGTTTCTTAGCCCTTTCAGAAGTTGAACAGTCCTATTGTTGCCAATGAAATCCATATTGGACGTGCATTATGAATACTAGGACAAAACCTATGATATTGCCTTAGATGTACATATTCCCTTCACCATTTGTTTTGGAGGACTAATACTTCTCATTTTGCTTAACCCATGCCTTGAAGGTTACCAATAATACTATTGCTATGTTTCTCAATTCAGTTCTAGAAAGTTTGTTTTGAAAGCATATATATGGAGTTAAACCTTGTCATACTTATTAATACAAGATTACGATCGCCCACTCTAATTCTGCCCTTTAAAAGAAAGAAAGAAAATATTTATTCATCGGATATGAATTCATAAAAAGAAAATATTTATTCATCGGATATGAATTCATCTTTCCTTGGCTCAGAGTTAAGAGAAGTAGAATATATTTTAATAGGCCTCCAATTCATCAACCTTAAAATTGGAAGGGGAAGAGGAGGCAAATATGGCATGAGTAGAGTAAGGGCTGGAGAGAATCTCGTAATATGGAACTACCAATAATTGTGAGGAAGTATAATAGAAGGGAAAGAGGGGGGAGAACTTTCTGGTAGAGTTGATTAGTAGGAGATGTTGGGACTTGGGAGCTTGCGCAGGTGAATCTCCCCAAATATGTAACCTCTGTCGATAGTAATTTCCACTTTGATCTCGATCATGTTCTCAAGTTCACAATTCCGGTCCCTTTATCGAGCCAACCTTTTATCATTACAACATACATATAATATCCTAGATGAACTTTTATTCATTCTTCTGGAAACTGAATTTGCCTGTTTGATCTCGATCATGTTCTCAAATCTCAAGTTCACAATTCCAGTCCCTTTATCGGGCCAACCTTTTATCATTACAACATACATATAATATCCTAGATGAACTTTATTCACTCTTCTGGAAACTGAATTTGTCTGTTTGATCTCGATCATGTTCTCAAATCTCAAGTTCACAATTCCAGTCCCTTTATCGGGCCAACCCTTTATCATTACAACATACATATAATATCCTAGATGAACTTTTATTCACTCTTCTGGAAACTGTATTTGTCTGTTTGATCTCGATCAAGTTCTCAAATCTCAAGTTCACAATTCCAGTCCCTTTATCGGGCCAACCCTTTATCATTACAACATACATATAATATCCTAGATGAACTTTTATTCACTCTTCTGGAAACTGAATTTGTCTGTTTGATCTCGATCAAGTTCTCAAATCTCTAGTCCACAATTCCAGCCCTTTATCGGGCCAACCTTTTATCATAACAACATACATATAATATCCTAGATGAACTTTTATTCTCTCTTCTGGAAACTGAAATTTGTCTGTTCATGCACATTTTTCCACAGAAAATATAATGTTCTAGATTATCTTAATCAGTGACATCGATTGTTTCAAGGTGAATCACGACATCAGTGTGGCTCTTACAGTATGTATAAGCACATATCAATCAACTTCATGCAGCTGTTGTTTAATTGTTGTTTCTGTTGTTCCTTCTATTGTTTTTAGAGTATTGGTGGACATACATGAGGGCGGATTCTGAAGGTTTACATGAGATCAGGAAGCTATGTGAAGCTGGAAAGCTGAAAATACCAGTCGAGAAAACATTTCCCATCTCACAAGTGAGAGAAGCCCACAACGCGAAGGATAAGAACCTCATTCCTGGTAAAGTGGTGCTAGAGCTGGAT

mRNA sequence

ATGCGAGCTCTACTGCGATGTCGCACCTCTGAATTCGAGCACGGATCGCCTTTTCTTGCTGGCTTTTGGTTTAGAACCATCGTCACCAGTTGCAGAGCCGTGGTTTTGCCCCGCTTCGGCGGTCCGGAGGTGCTCCAGCTCCGTCCCGACGTTGACGTCCCCAATCTCAAGCCTAATGAGGTTCTCGTCCGTGCTCGTGCTGTTTCCATCAACCCGCTTGATACCAGAATGCGATCAGGTTATGGTCGCTCAATATTTGAACCACTTCTACCGGTCATTTTGGGCCGTGATATCAGTGGTGAAGTTGCGGCTGTTGGTTCTTCAGTTCGTTCAGTAAGTGTTGGAGAAGAAGTTTTTGGTGCTCTGCATCCAACTGCTGTGAGGGGTACTTACGCTGATTATGCAATACTTTCAGACGATGAATTAAGTGCTAAACCAGTGTCATTTACACATGTGGAGGCAAGTGCCATTCCTTTTGCTGCACTGACTGCATGGCGGGCTCTAAAAAGTACTGCTAGAATCACCCAGGGGCATAGGGTATTAGTAATTGGTGGTGGAGGAGCCGTAGGTTCTGCTGCAGTTCAGATTTCAGTTGCTTCAGGCTGCCACGTCACAACTACTTGTGGAAGTCAAAGTATAGATAAATTACTTGCAGCAGGTGCTGAGCAAGCAGTTGACTATACTACCGAGGACATTGAATTGGTAATAAAGGGGAAATTTGATGCTGTTTTGGATACCATTGGTGCGCCAGATACTGAACGAATTGGCATCAGTCTTTTGAAGAGAGGTGGACACTATATGACACTTCAGGGTGAGGCTGCATCATTCACTGATAAATATGGGCTACTTTTCGGGCTCCCTATAGCTACAAGTGTATTGGCGAAGAAACAGATACAGTACAGATACTCTCATGGAATCGAGTATTGGTGGACATACATGAGGGCGGATTCTGAAGGTTTACATGAGATCAGGAAGCTATGTGAAGCTGGAAAGCTGAAAATACCAGTCGAGAAAACATTTCCCATCTCACAAGTGAGAGAAGCCCACAACGCGAAGGATAAGAACCTCATTCCTGGTAAAGTGGTGCTAGAGCTGGAT

Coding sequence (CDS)

ATGCGAGCTCTACTGCGATGTCGCACCTCTGAATTCGAGCACGGATCGCCTTTTCTTGCTGGCTTTTGGTTTAGAACCATCGTCACCAGTTGCAGAGCCGTGGTTTTGCCCCGCTTCGGCGGTCCGGAGGTGCTCCAGCTCCGTCCCGACGTTGACGTCCCCAATCTCAAGCCTAATGAGGTTCTCGTCCGTGCTCGTGCTGTTTCCATCAACCCGCTTGATACCAGAATGCGATCAGGTTATGGTCGCTCAATATTTGAACCACTTCTACCGGTCATTTTGGGCCGTGATATCAGTGGTGAAGTTGCGGCTGTTGGTTCTTCAGTTCGTTCAGTAAGTGTTGGAGAAGAAGTTTTTGGTGCTCTGCATCCAACTGCTGTGAGGGGTACTTACGCTGATTATGCAATACTTTCAGACGATGAATTAAGTGCTAAACCAGTGTCATTTACACATGTGGAGGCAAGTGCCATTCCTTTTGCTGCACTGACTGCATGGCGGGCTCTAAAAAGTACTGCTAGAATCACCCAGGGGCATAGGGTATTAGTAATTGGTGGTGGAGGAGCCGTAGGTTCTGCTGCAGTTCAGATTTCAGTTGCTTCAGGCTGCCACGTCACAACTACTTGTGGAAGTCAAAGTATAGATAAATTACTTGCAGCAGGTGCTGAGCAAGCAGTTGACTATACTACCGAGGACATTGAATTGGTAATAAAGGGGAAATTTGATGCTGTTTTGGATACCATTGGTGCGCCAGATACTGAACGAATTGGCATCAGTCTTTTGAAGAGAGGTGGACACTATATGACACTTCAGGGTGAGGCTGCATCATTCACTGATAAATATGGGCTACTTTTCGGGCTCCCTATAGCTACAAGTGTATTGGCGAAGAAACAGATACAGTACAGATACTCTCATGGAATCGAGTATTGGTGGACATACATGAGGGCGGATTCTGAAGGTTTACATGAGATCAGGAAGCTATGTGAAGCTGGAAAGCTGAAAATACCAGTCGAGAAAACATTTCCCATCTCACAAGTGAGAGAAGCCCACAACGCGAAGGATAAGAACCTCATTCCTGGTAAAGTGGTGCTAGAGCTGGAT

Protein sequence

MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKFDAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLELD
Homology
BLAST of MS002750 vs. NCBI nr
Match: XP_023553198.1 (reticulon-4-interacting protein 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.6 bits (1677), Expect = 8.1e-183
Identity = 330/366 (90.16%), Postives = 345/366 (94.26%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCRTS+FEHGSPFLAG  FRTIVT+CRAVVLP FGGPEVLQLRPDV+VP+LKP+E
Sbjct: 1   MRALLRCRTSKFEHGSPFLAG--FRTIVTTCRAVVLPHFGGPEVLQLRPDVEVPSLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  +KGKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTLKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVLGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QVREAH AKDKN+IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVREAHEAKDKNIIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. NCBI nr
Match: KAG7015031.1 (Reticulon-4-interacting protein 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.0 bits (1673), Expect = 2.4e-182
Identity = 330/366 (90.16%), Postives = 343/366 (93.72%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR S+FEHGSPFLAG  FRTIVT+CRAVVLP FGGPEVLQLR DVDVPNLKP+E
Sbjct: 1   MRALLRCRASKFEHGSPFLAG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVDVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  +KGKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTLKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVLGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QVREAH AKDKN+IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVREAHEAKDKNIIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. NCBI nr
Match: KAG6577009.1 (Reticulon-4-interacting protein 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-182
Identity = 329/366 (89.89%), Postives = 343/366 (93.72%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR S+FEHGSPFLAG  FRTIVT+CRAVVLP FGGPEVLQLR DVDVPNLKP+E
Sbjct: 1   MRALLRCRASKFEHGSPFLAG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVDVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEA+AIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEATAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  +KGKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTLKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVLGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QVREAH AKDKN+IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVREAHEAKDKNIIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. NCBI nr
Match: XP_022922906.1 (reticulon-4-interacting protein 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 644.8 bits (1662), Expect = 4.4e-181
Identity = 329/366 (89.89%), Postives = 340/366 (92.90%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR SEFEHGSPFL G  FRTIVT+CRAVVLP FGGPEVLQLR DVDVPNLKP+E
Sbjct: 1   MRALLRCRASEFEHGSPFLDG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVDVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYG SIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGSSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  +KGKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTLKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVLGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QVREAH AKDKN IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVREAHEAKDKNTIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. NCBI nr
Match: XP_022984733.1 (reticulon-4-interacting protein 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 641.3 bits (1653), Expect = 4.9e-180
Identity = 327/366 (89.34%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR S+FEH SPFLAG  FRTIVT+CRAVVLP FGGPEVLQLR DV+VPNLKP+E
Sbjct: 1   MRALLRCRASKFEHRSPFLAG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVEVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  ++GKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTMEGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+FGLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVFGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QV EAH AKDKN IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVMEAHEAKDKNTIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. ExPASy Swiss-Prot
Match: Q924D0 (Reticulon-4-interacting protein 1, mitochondrial OS=Mus musculus OX=10090 GN=Rtn4ip1 PE=1 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 2.0e-51
Identity = 126/382 (32.98%), Postives = 207/382 (54.19%), Query Frame = 0

Query: 20  AGFWFRTIV----------TSCRAVVLP-----RFGGPEVLQLRPDVDVPNLK-PNEVLV 79
           A FW RT++          TS R+ V+P     ++G  EVL+   ++ +P +  PNEV++
Sbjct: 17  ACFWRRTVIPKPPFRGISTTSARSTVMPAWVIDKYGKNEVLRFTQNMMLPIIHYPNEVII 76

Query: 80  RARAVSINPLDTRMRSGYGRSIF----EPL--------LPVILGRDISGEVAAVGSSVRS 139
           +  A S+NP+D  MRSGYG +      +PL         P+ LGRD+SG V   G  V+ 
Sbjct: 77  KVHAASVNPIDVNMRSGYGATALNMKRDPLHMKTKGEEFPLTLGRDVSGVVMECGLDVKY 136

Query: 140 VSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKST 199
              G+EV+ A+ P   +GT +++ ++S +E+S KP S TH +A+++P+ ALTAW A+   
Sbjct: 137 FQPGDEVWAAVPPWK-QGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKV 196

Query: 200 ARIT----QGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDY 259
             ++    +G R L++G  G VG+ A+Q+  A G HVT  C   + + +   GA++ +DY
Sbjct: 197 GGLSDKNCKGKRALILGASGGVGTFAIQVMKAWGAHVTAVCSKDASELVRKLGADEVIDY 256

Query: 260 TTEDIELVIKG--KFDAVLDTIGAPDTERIGISLLKR--GGHYMTLQGEAASFTDKYGLL 319
           T   +E  +K    FD +LD +G   TE   ++ LK+  G  Y+TL        D+ G+ 
Sbjct: 257 TLGSVEEQLKSLKLFDFILDNVGG-STETWALNFLKKWSGATYVTLVTPFLLNMDRLGVA 316

Query: 320 FGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPIS 366
            G+      +  K +++ +  G+ Y W +  A    L EI +L +AGK++  +E+TFP S
Sbjct: 317 DGMLQTGVTVGTKALKHLW-QGVHYRWAFFMASGPYLDEIAELVDAGKIRPVIERTFPFS 376

BLAST of MS002750 vs. ExPASy Swiss-Prot
Match: Q0VC50 (Reticulon-4-interacting protein 1, mitochondrial OS=Bos taurus OX=9913 GN=RTN4IP1 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.7e-51
Identity = 125/363 (34.44%), Postives = 196/363 (53.99%), Query Frame = 0

Query: 29  TSCRAVVLP-----RFGGPEVLQLRPDVDVPNLK-PNEVLVRARAVSINPLDTRMRSGYG 88
           TS R+ V+P     ++G  EVL+   ++ +P +  PNEV+++  A SINP+D  MRSGYG
Sbjct: 36  TSPRSTVMPAWVIDKYGSNEVLRFTQNMMIPMIHYPNEVIIKVHAASINPIDVNMRSGYG 95

Query: 89  RSIF----EPL--------LPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGT 148
            +      +PL         P+ LGRD+SG V   G  VR    G+EV+ A+ P   +GT
Sbjct: 96  ATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVRYFKPGDEVWAAVPPWK-QGT 155

Query: 149 YADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTA----RITQGHRVLVIGGG 208
            +++ ++S +E+S KP S TH +A+++P+ ALTAW A+        R   G RVL++G  
Sbjct: 156 LSEFVVVSGNEVSHKPRSLTHTQAASLPYVALTAWSAINKVGGLNDRNCTGKRVLILGAS 215

Query: 209 GAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGK--FDAVL 268
           G VG+ A+Q+  A   HVT  C   + + +   GA+  +DY + ++E  +K    FD +L
Sbjct: 216 GGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGNVEAQLKSSKPFDFIL 275

Query: 269 DTIGAPDTERIGISLLKR--GGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQYRY 328
           D +G   TE   +  LK+  G  Y+TL        D+ G+  G+ + T V    +    +
Sbjct: 276 DNVGG-STETWALKFLKKWSGATYVTLVTPFLLNMDRLGIADGM-LQTGVTVGSKTLKHF 335

Query: 329 SHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVV 366
             G+ Y W +  A    L +I +L EAGK++  +EKTFP S+V EA    ++    GK V
Sbjct: 336 WQGVHYRWAFFMASGPCLDDIAELVEAGKIQPVIEKTFPFSKVPEAFLKVERGHARGKTV 395

BLAST of MS002750 vs. ExPASy Swiss-Prot
Match: Q7T3C7 (Reticulon-4-interacting protein 1 homolog, mitochondrial OS=Danio rerio OX=7955 GN=rtn4ip1 PE=2 SV=2)

HSP 1 Score: 195.7 bits (496), Expect = 9.4e-49
Identity = 123/355 (34.65%), Postives = 201/355 (56.62%), Query Frame = 0

Query: 33  AVVLPRFGGPEVLQLRPDVDVPNLK-PNEVLVRARAVSINPLDTRMRSGYGRSIF----E 92
           A V+ ++G  +VL+   +  +P +  PNEV+V+  A  +NP+D  MR GYG +      +
Sbjct: 31  AWVIDKYGKNDVLRFTKNAALPIIHYPNEVVVKVHAAGLNPIDISMRGGYGAATMAMKRD 90

Query: 93  PL--------LPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSD 152
           PL         P+ILGRD+SGE+   G  V+    G++V+ A+ P   +G+ A++ ++S 
Sbjct: 91  PLNISQSGGEFPLILGRDVSGEIMECGLDVKYFKPGDQVWAAIPPWK-QGSLAEFVVVSG 150

Query: 153 DELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGH----RVLVIGGGGAVGSAAVQ 212
           +E+S KP S  H EA++IP+ A TAW A+ +T  + + +    RVL++GG G VG+ A+Q
Sbjct: 151 NEVSHKPKSLRHDEAASIPYVAATAWSAIVNTGGLNKDNSAKKRVLILGGSGGVGTFAIQ 210

Query: 213 ISVASGCHVTTTCGSQSIDKLLA-AGAEQAVDYTTEDIELVIKG--KFDAVLDTIGAPDT 272
           +  A G HVT TC SQ+ ++L+   GA+  VDYT   +E  +K   KFD +LD+IG  +T
Sbjct: 211 MVKAWGAHVTVTC-SQNAERLVRDLGADDVVDYTAGPVEKQLKNLEKFDLILDSIGG-ET 270

Query: 273 ERIGISLLK--RGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWW 332
           E+  + LLK   G  ++TL       TD+ GL  G+ + ++V    ++      G+ Y W
Sbjct: 271 EKWALDLLKPWSGAKFVTLITPFLQNTDRLGLADGM-MQSAVTVGCKVVKNLRKGVHYRW 330

Query: 333 TYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLEL 366
            +       L E+ ++ +AGK++  VE+ F  +QV EA    ++    GK V+ +
Sbjct: 331 GFFAPSGSALDEVSEMVDAGKVRPVVEEVFSFAQVPEAFQKVEQGHARGKTVVSI 381

BLAST of MS002750 vs. ExPASy Swiss-Prot
Match: Q8WWV3 (Reticulon-4-interacting protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=RTN4IP1 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-48
Identity = 122/367 (33.24%), Postives = 195/367 (53.13%), Query Frame = 0

Query: 25  RTIVTSCRAVVLP-----RFGGPEVLQLRPDVDVPNLK-PNEVLVRARAVSINPLDTRMR 84
           R   TS R+ V+P     ++G  EVL+   ++ +P +  PNEV+V+  A S+NP+D  MR
Sbjct: 32  RISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNMR 91

Query: 85  SGYGRSIF----EPL--------LPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTA 144
           SGYG +      +PL         P+ LGRD+SG V   G  V+    G+EV+ A+ P  
Sbjct: 92  SGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPGDEVWAAVPPWK 151

Query: 145 VRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQ----GHRVLV 204
            +GT +++ ++S +E+S KP S TH +A+++P+ ALTAW A+     +      G RVL+
Sbjct: 152 -QGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLI 211

Query: 205 IGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKG--KF 264
           +G  G VG+ A+Q+  A   HVT  C   + + +   GA+  +DY +  +E  +K    F
Sbjct: 212 LGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPF 271

Query: 265 DAVLDTIGAPDTERIGISLLKR--GGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQI 324
           D +LD +G   TE      LK+  G  Y+TL        D+ G+  G+ + T V    + 
Sbjct: 272 DFILDNVGG-STETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGM-LQTGVTVGSKA 331

Query: 325 QYRYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIP 366
              +  G+ Y W +  A    L +I +L +AGK++  +E+TFP S+V EA    ++    
Sbjct: 332 LKHFWKGVHYRWAFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHAR 391

BLAST of MS002750 vs. ExPASy Swiss-Prot
Match: Q54II4 (Reticulon-4-interacting protein 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=rtn4ip1 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.0e-39
Identity = 104/336 (30.95%), Postives = 181/336 (53.87%), Query Frame = 0

Query: 43  EVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSI--FEPLLPVILGRDISG 102
           ++L+ + D+ VP    ++VL++  + SINPLD  MR GY  SI   +  LP+ILGR+ SG
Sbjct: 14  DLLEYKTDLPVPKPIKSQVLIKIHSTSINPLDNVMRKGYASSIVDLKLKLPIILGRECSG 73

Query: 103 EVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFA 162
           E+  +G SV    +G++V+ A  P ++ G++ +Y  + + E+S KP + TH ++++IPFA
Sbjct: 74  EIVEIGDSVWDYEIGDQVWSASPPFSM-GSHCEYITVDESEISLKPKNLTHQQSASIPFA 133

Query: 163 ALTAWRA----LKSTARITQGHRVLVIGGGGAVGSAAVQI--SVASGCHVTTTCGSQSID 222
           +LTAW A    L +  +IT   ++LV GG G+VG   +Q+     +   V+TTC  +  +
Sbjct: 134 SLTAWNAIYNVLPTNKKITTNTKILVNGGNGSVGFFILQLLKKHLNVNQVSTTCNIKHFE 193

Query: 223 KLLAAG-AEQAVDYTTEDIELVIKGKFDAVLDTI----GAPDTERIGISLLKRGGHYMTL 282
           KL       + +DY    I      KFD + +         + E+  I  LK GG+ +  
Sbjct: 194 KLKKLTLVNETIDYNNLKINDNDNNKFDLIFNCYDGGKNQNENEKKCIDALKDGGNLIGF 253

Query: 283 QGEAASFTDKYGLLFGLPIA--TSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIRKLC 342
            G    F+DK G+L GLP+     + + ++I+ +YS  +   +         L +I KL 
Sbjct: 254 NGPLVKFSDKDGVLSGLPMGMMNQLNSSERIKKQYSKNVHLDYAIFSPSGSTLKQISKLY 313

Query: 343 EAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVL 364
           E   L   ++K F ++Q+++A+   + +   GK+++
Sbjct: 314 ENNILIPNIDKQFNLNQIKDAYTCFENSNSNGKIII 348

BLAST of MS002750 vs. ExPASy TrEMBL
Match: A0A6J1E5E5 (reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111430742 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.2e-181
Identity = 329/366 (89.89%), Postives = 340/366 (92.90%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR SEFEHGSPFL G  FRTIVT+CRAVVLP FGGPEVLQLR DVDVPNLKP+E
Sbjct: 1   MRALLRCRASEFEHGSPFLDG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVDVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYG SIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGSSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  +KGKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTLKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVLGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QVREAH AKDKN IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVREAHEAKDKNTIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. ExPASy TrEMBL
Match: A0A6J1JBD9 (reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111482921 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 327/366 (89.34%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           MRALLRCR S+FEH SPFLAG  FRTIVT+CRAVVLP FGGPEVLQLR DV+VPNLKP+E
Sbjct: 1   MRALLRCRASKFEHRSPFLAG--FRTIVTTCRAVVLPHFGGPEVLQLRSDVEVPNLKPDE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG
Sbjct: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS DELSAKP SFTHVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSADELSAKPGSFTHVEASAIPFAALTAWRALKSTARIKEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQISVASGCHV TTCGSQSIDKLLAAGAEQAVDYTTE IE  ++GKF
Sbjct: 181 LVIGGGGAVGYAAVQISVASGCHVATTCGSQSIDKLLAAGAEQAVDYTTEGIEFTMEGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+FGLPIATS+L +KQ+QY
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGLVFGLPIATSILLQKQLQY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R SHGIEYWWTYMRADSEGL EIR+LCEAGKLKIPVEKTFPI+QV EAH AKDKN IPGK
Sbjct: 301 RCSHGIEYWWTYMRADSEGLDEIRRLCEAGKLKIPVEKTFPITQVMEAHEAKDKNTIPGK 360

Query: 361 VVLELD 367
           VVLELD
Sbjct: 361 VVLELD 364

BLAST of MS002750 vs. ExPASy TrEMBL
Match: A0A0A0L6P2 (PKS_ER domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G236570 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.9e-169
Identity = 309/366 (84.43%), Postives = 333/366 (90.98%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           M ALL   TS+F   SPFL    FRT+VT+CRAV+LP FGGPEVLQLR DV VP LKP+E
Sbjct: 1   MPALLPSPTSKFRLRSPFLIS-GFRTLVTTCRAVLLPHFGGPEVLQLRSDVPVPILKPHE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVR+ AVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVG+EVFG
Sbjct: 61  VLVRSCAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGDEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS++ELSAKPVSF+HVEASAIPFAALTAWRALKSTARIT+G RV
Sbjct: 121 ALHPTAVRGTYADYAILSEEELSAKPVSFSHVEASAIPFAALTAWRALKSTARITEGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQ+SVASGCHV TTCGSQSI+KLLAAGAEQAVDYTTE IE  IKGKF
Sbjct: 181 LVIGGGGAVGYAAVQLSVASGCHVATTCGSQSIEKLLAAGAEQAVDYTTEGIEFKIKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YG++ GLP +T VL +KQ++Y
Sbjct: 241 DAVLDTIGVPDTERIGIHLLNRGGHYMTLQGEAASLTDRYGVVLGLPFSTGVLLQKQLKY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R+SHGIEYWWTYMRADSEGL EIR+LCEA KLKIPVEKTFPI+QVREAH+AKDKNLIPGK
Sbjct: 301 RFSHGIEYWWTYMRADSEGLEEIRRLCEAEKLKIPVEKTFPITQVREAHDAKDKNLIPGK 360

Query: 361 VVLELD 367
           VVLE D
Sbjct: 361 VVLEFD 365

BLAST of MS002750 vs. ExPASy TrEMBL
Match: A0A5D3CLG4 (Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001120 PE=4 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.4e-167
Identity = 307/366 (83.88%), Postives = 329/366 (89.89%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           M  LLR  TS+    SPFL    FRT+VT+CRAV+LP FGGPEVLQLR DV VP LKP+E
Sbjct: 1   MPTLLRSPTSKSRLLSPFLIS-GFRTLVTTCRAVLLPHFGGPEVLQLRSDVPVPILKPHE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVR RAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVG+EVFG
Sbjct: 61  VLVRTRAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGDEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS++ELSAKPVS +HVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSEEELSAKPVSLSHVEASAIPFAALTAWRALKSTARIREGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQ+SVASGCHV TTCGSQSI+KLLAAGAEQAVDYTTE IE  IKGKF
Sbjct: 181 LVIGGGGAVGYAAVQLSVASGCHVATTCGSQSIEKLLAAGAEQAVDYTTEGIEFKIKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLP +T VL +KQ++Y
Sbjct: 241 DAVLDTIGVPDTERIGIYLLNRGGHYMTLQGEAASLTDRYGLVLGLPFSTGVLLQKQLKY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R+SH IEYWWTYMRADSEGL EIR+LCEA KLKIPVEKTFPI+QVREAH+AKDKNLIPGK
Sbjct: 301 RFSHAIEYWWTYMRADSEGLEEIRRLCEAEKLKIPVEKTFPITQVREAHDAKDKNLIPGK 360

Query: 361 VVLELD 367
           VVLE D
Sbjct: 361 VVLEFD 365

BLAST of MS002750 vs. ExPASy TrEMBL
Match: A0A5A7UZ93 (Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00370 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.0e-167
Identity = 306/366 (83.61%), Postives = 329/366 (89.89%), Query Frame = 0

Query: 1   MRALLRCRTSEFEHGSPFLAGFWFRTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNE 60
           M  LLR  TS+    SPFL    FRT+VT+CRAV+LP FGGPEVLQLR DV VP LKP+E
Sbjct: 1   MPTLLRSPTSKSRLLSPFLIS-GFRTLVTTCRAVLLPHFGGPEVLQLRSDVPVPILKPHE 60

Query: 61  VLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFG 120
           VLVR RAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVG+EVFG
Sbjct: 61  VLVRTRAVSINPLDTRMRSGYGRSIFEPLLPVILGRDISGEVAAVGSSVRSVSVGDEVFG 120

Query: 121 ALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRV 180
           ALHPTAVRGTYADYAILS++ELSAKPVS +HVEASAIPFAALTAWRALKSTARI +G RV
Sbjct: 121 ALHPTAVRGTYADYAILSEEELSAKPVSLSHVEASAIPFAALTAWRALKSTARIREGQRV 180

Query: 181 LVIGGGGAVGSAAVQISVASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKF 240
           LVIGGGGAVG AAVQ+SVASGCHV TTCGSQSI+KLLAAG+EQAVDYTTE IE  IKGKF
Sbjct: 181 LVIGGGGAVGYAAVQLSVASGCHVATTCGSQSIEKLLAAGSEQAVDYTTEGIEFKIKGKF 240

Query: 241 DAVLDTIGAPDTERIGISLLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQY 300
           DAVLDTIG PDTERIGI LL RGGHYMTLQGEAAS TD+YGL+ GLP +T VL +KQ++Y
Sbjct: 241 DAVLDTIGVPDTERIGIYLLNRGGHYMTLQGEAASLTDRYGLVLGLPFSTGVLLQKQLKY 300

Query: 301 RYSHGIEYWWTYMRADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGK 360
           R+SH IEYWWTYMRADSEGL EIR+LCEA KLKIPVEKTFPI+QVREAH+AKDKNLIPGK
Sbjct: 301 RFSHAIEYWWTYMRADSEGLEEIRRLCEAEKLKIPVEKTFPITQVREAHDAKDKNLIPGK 360

Query: 361 VVLELD 367
           VVLE D
Sbjct: 361 VVLEFD 365

BLAST of MS002750 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 525.4 bits (1352), Expect = 3.7e-149
Identity = 251/340 (73.82%), Postives = 301/340 (88.53%), Query Frame = 0

Query: 25  RTIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRS 84
           R++ T CRAV+LPRFGGPEV +LR +V VPNL PNEVLV+A+AVS+NPLD R+R+GYGRS
Sbjct: 26  RSVFTGCRAVILPRFGGPEVFELRENVPVPNLNPNEVLVKAKAVSVNPLDCRIRAGYGRS 85

Query: 85  IFEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSA 144
           +F+P LP+I+GRD+SGEVAA+G+SV+S+ VG+EVFGALHPTA+RGTY DY ILS+DEL+ 
Sbjct: 86  VFQPHLPIIVGRDVSGEVAAIGTSVKSLKVGQEVFGALHPTALRGTYTDYGILSEDELTE 145

Query: 145 KPVSFTHVEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQISVASGCHV 204
           KP S +HVEASAIPFAALTAWRALKS ARIT+G R+LV GGGGAVG +A+Q++VASGCHV
Sbjct: 146 KPSSISHVEASAIPFAALTAWRALKSNARITEGQRLLVFGGGGAVGFSAIQLAVASGCHV 205

Query: 205 TTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKFDAVLDTIGAPDTERIGISLLKRGG 264
           T +C  Q+ D++LAAGAEQAVDYTTEDIEL +KGKFDAVLDTIG P+TERIGI+ L++GG
Sbjct: 206 TASCVGQTKDRILAAGAEQAVDYTTEDIELAVKGKFDAVLDTIGGPETERIGINFLRKGG 265

Query: 265 HYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIR 324
           +YMTLQGEAAS TDKYG + GLP+ATS+L KK+IQY+YSHGI+YWWTYMRAD EGL EI+
Sbjct: 266 NYMTLQGEAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRADPEGLAEIQ 325

Query: 325 KLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLE 365
           +L  AGKLKIPVEKTFPI+ V  AH AK+K  IPGKVVLE
Sbjct: 326 RLVGAGKLKIPVEKTFPITDVVAAHEAKEKKQIPGKVVLE 365

BLAST of MS002750 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-31
Identity = 98/347 (28.24%), Postives = 172/347 (49.57%), Query Frame = 0

Query: 26  TIVTSCRAVVLPRFGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSI 85
           +I    +A V   +GG +VL+L  ++ VP +K ++VL++  A ++NP+D + R G  ++ 
Sbjct: 73  SIPKEMKAWVYSDYGGVDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGKFKAT 132

Query: 86  FEPLLPVILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVR-----GTYADYAILSDD 145
             P LP + G D++G V  VGS+V+ +  G+EV+  +   A+      G+ A+Y  + + 
Sbjct: 133 DSP-LPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAVEEK 192

Query: 146 ELSAKPVSFTHVEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQIS--V 205
            L+ KP +    +A+ +P A  TA   L  T   + G  +LV+ G G VGS  +Q++  V
Sbjct: 193 LLALKPKNIDFAQAAGLPLAIETADEGLVRT-EFSAGKSILVLNGAGGVGSLVIQLAKHV 252

Query: 206 ASGCHVTTTCGSQSIDKLLAAGAEQAVDYTTEDIELVIKGKFDAVLDTIGAPDTERIGIS 265
                V  T  ++ ++ + + GA+ A+DYT E+IE  +  K+D V D IG  D     + 
Sbjct: 253 YGASKVAATASTEKLELVRSLGADLAIDYTKENIE-DLPDKYDVVFDAIGMCDK---AVK 312

Query: 266 LLKRGGHYMTLQGEAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSE 325
           ++K GG  + L G                      A     +R+          + ++ +
Sbjct: 313 VIKEGGKVVALTG----------------------AVTPPGFRF---------VVTSNGD 372

Query: 326 GLHEIRKLCEAGKLKIPVEK--TFPISQVREAHNAKDKNLIPGKVVL 364
            L ++    E+GK+K  V+    FP S+V +A +  + N   GKVV+
Sbjct: 373 VLKKLNPYIESGKVKPVVDPKGPFPFSRVADAFSYLETNHATGKVVV 382

BLAST of MS002750 vs. TAIR 10
Match: AT4G21580.1 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-25
Identity = 104/350 (29.71%), Postives = 156/350 (44.57%), Query Frame = 0

Query: 32  RAVVLPRFGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSIFEPLLP 91
           +A+V+   G PEVLQLR DV  P +K +EVL+R  A ++N  DT  R G       P   
Sbjct: 2   KAIVISEPGKPEVLQLR-DVADPEVKDDEVLIRVLATALNRADTLQRLGLYNP--PPGSS 61

Query: 92  VILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTH 151
             LG + SG + +VG  V    VG++V   L      G YA+   +   ++   P   + 
Sbjct: 62  PYLGLECSGTIESVGKGVSRWKVGDQVCALLS----GGGYAEKVSVPAGQIFPIPAGISL 121

Query: 152 VEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQ 211
            +A+A P  A T W  +    R++ G   L+ GG   +G+ A+QI+   G  V  T GS 
Sbjct: 122 KDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHLGVRVFVTAGS- 181

Query: 212 SIDKLLAA----GAEQAVDYTTEDIELVIKGK-----FDAVLDTIGAPDTERIGISLLKR 271
             D+ LAA    GA+  ++Y TED    +K +      D +LD IGAP  ++  +  L  
Sbjct: 182 --DEKLAACKELGADVCINYKTEDFVAKVKAETDGKGVDVILDCIGAPYLQK-NLDSLNF 241

Query: 272 GGH--YMTLQGEA------ASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMR 331
            G    + L G A      +S   K   + G  +       K +  R             
Sbjct: 242 DGRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVR------------- 301

Query: 332 ADSEGLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLE 365
              E    +    EAGK+K  + K  P+SQ  E H+  + +   GK++LE
Sbjct: 302 ---EVEKNVWPAIEAGKVKPVIYKYLPLSQAAEGHSLMESSNHIGKILLE 324

BLAST of MS002750 vs. TAIR 10
Match: AT4G13010.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.5e-25
Identity = 87/334 (26.05%), Postives = 155/334 (46.41%), Query Frame = 0

Query: 39  FGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSIFEPLLPVILGRDI 98
           +GG         V VP  K NEV ++  A S+NP+D +++ G  R       P I   D+
Sbjct: 14  YGGGAAGLEHVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPFLPRKFPCIPATDV 73

Query: 99  SGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTHVEASAIP 158
           +GEV  VGS V++   G++V   L      G  A++A+ ++     +P      EA+A+P
Sbjct: 74  AGEVVEVGSGVKNFKAGDKVVAVLSHLG-GGGLAEFAVATEKLTVKRPQEVGAAEAAALP 133

Query: 159 FAALTAWRALKSTARIT-----QGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQSI 218
            A LTA +AL + A +      +   +LV    G VG  AVQ++  +  HVT TCG+++I
Sbjct: 134 VAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLAKLANAHVTATCGARNI 193

Query: 219 DKLLAAGAEQAVDYTTED---IELVIKGKFDAVLDTIGAPDTERIGISLLKRGGHYMTLQ 278
           + + + GA++ +DY T +   ++     K+DAV+          I  S+ +     ++  
Sbjct: 194 EFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANG-----IPFSVFEPN---LSEN 253

Query: 279 GEAASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSEGLHEIRKLCEAG 338
           G+    T     ++   +    ++KKQ+              +   +E L  +  L + G
Sbjct: 254 GKVIDITPGPNAMWTYAVKKITMSKKQLVP----------LLLIPKAENLEFMVNLVKEG 313

Query: 339 KLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLE 365
           K+K  ++   P+S+  +A          GK+++E
Sbjct: 314 KVKTVIDSKHPLSKAEDAWAKSIDGHATGKIIVE 328

BLAST of MS002750 vs. TAIR 10
Match: AT4G21580.3 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-23
Identity = 98/346 (28.32%), Postives = 150/346 (43.35%), Query Frame = 0

Query: 32  RAVVLPRFGGPEVLQLRPDVDVPNLKPNEVLVRARAVSINPLDTRMRSGYGRSIFEPLLP 91
           +A+V+   G PEVLQLR DV  P +K +EVL+R  A ++N  DT  R G       P   
Sbjct: 2   KAIVISEPGKPEVLQLR-DVADPEVKDDEVLIRVLATALNRADTLQRLGLYNP--PPGSS 61

Query: 92  VILGRDISGEVAAVGSSVRSVSVGEEVFGALHPTAVRGTYADYAILSDDELSAKPVSFTH 151
             LG + SG + +VG  V    VG++V   L      G YA+   +   ++   P   + 
Sbjct: 62  PYLGLECSGTIESVGKGVSRWKVGDQVCALLS----GGGYAEKVSVPAGQIFPIPAGISL 121

Query: 152 VEASAIPFAALTAWRALKSTARITQGHRVLVIGGGGAVGSAAVQISVASGCHVTTTCGSQ 211
            +A+A P  A T W  +    R++ G   L+ GG   +G+ A+QI+   G  V  T  + 
Sbjct: 122 KDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHLGVRVFVTAAA- 181

Query: 212 SIDKLLAAGAEQAVDYTTEDIELVIKGK-----FDAVLDTIGAPDTERIGISLLKRGGH- 271
                   GA+  ++Y TED    +K +      D +LD IGAP  ++  +  L   G  
Sbjct: 182 ----CKELGADVCINYKTEDFVAKVKAETDGKGVDVILDCIGAPYLQK-NLDSLNFDGRL 241

Query: 272 -YMTLQGEA------ASFTDKYGLLFGLPIATSVLAKKQIQYRYSHGIEYWWTYMRADSE 331
             + L G A      +S   K   + G  +       K +  R                E
Sbjct: 242 CIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVR----------------E 301

Query: 332 GLHEIRKLCEAGKLKIPVEKTFPISQVREAHNAKDKNLIPGKVVLE 365
               +    EAGK+K  + K  P+SQ  E H+  + +   GK++LE
Sbjct: 302 VEKNVWPAIEAGKVKPVIYKYLPLSQAAEGHSLMESSNHIGKILLE 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553198.18.1e-18390.16reticulon-4-interacting protein 1, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG7015031.12.4e-18290.16Reticulon-4-interacting protein 1, mitochondrial [Cucurbita argyrosperma subsp. ... [more]
KAG6577009.15.3e-18289.89Reticulon-4-interacting protein 1, mitochondrial, partial [Cucurbita argyrosperm... [more]
XP_022922906.14.4e-18189.89reticulon-4-interacting protein 1, mitochondrial [Cucurbita moschata][more]
XP_022984733.14.9e-18089.34reticulon-4-interacting protein 1, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q924D02.0e-5132.98Reticulon-4-interacting protein 1, mitochondrial OS=Mus musculus OX=10090 GN=Rtn... [more]
Q0VC507.7e-5134.44Reticulon-4-interacting protein 1, mitochondrial OS=Bos taurus OX=9913 GN=RTN4IP... [more]
Q7T3C79.4e-4934.65Reticulon-4-interacting protein 1 homolog, mitochondrial OS=Danio rerio OX=7955 ... [more]
Q8WWV32.1e-4833.24Reticulon-4-interacting protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=RTN4... [more]
Q54II41.0e-3930.95Reticulon-4-interacting protein 1 homolog, mitochondrial OS=Dictyostelium discoi... [more]
Match NameE-valueIdentityDescription
A0A6J1E5E52.2e-18189.89reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JBD92.4e-18089.34reticulon-4-interacting protein 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0L6P21.9e-16984.43PKS_ER domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G236570 PE=4... [more]
A0A5D3CLG41.4e-16783.88Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7UZ933.0e-16783.61Reticulon-4-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
AT3G15090.13.7e-14973.82GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT1G23740.11.4e-3128.24Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT4G21580.11.1e-2529.71oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT4G13010.12.5e-2526.05Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT4G21580.31.4e-2328.32oxidoreductase, zinc-binding dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020843Polyketide synthase, enoylreductase domainSMARTSM00829PKS_ER_names_modcoord: 40..363
e-value: 3.7E-11
score: -0.2
NoneNo IPR availablePFAMPF13602ADH_zinc_N_2coord: 220..363
e-value: 1.9E-19
score: 71.0
NoneNo IPR availableGENE3D3.90.180.10coord: 38..363
e-value: 2.6E-94
score: 318.2
NoneNo IPR availableGENE3D3.40.50.720coord: 157..335
e-value: 2.6E-94
score: 318.2
NoneNo IPR availablePANTHERPTHR43482:SF1PROTEIN AST1-RELATEDcoord: 28..365
NoneNo IPR availablePANTHERPTHR43482PROTEIN AST1-RELATEDcoord: 28..365
IPR013154Alcohol dehydrogenase, N-terminalPFAMPF08240ADH_Ncoord: 58..120
e-value: 5.4E-8
score: 32.7
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 28..181
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 150..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002750.1MS002750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity