MS002723 (gene) Bitter gourd (TR) v1

Overview


Name	MS002723
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	vicilin-like seed storage protein At2g18540
Location	scaffold66: 28460 .. 30899 (-)
RNA-Seq Expression	MS002723
Synteny	MS002723

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGAGAACGGAGCCCCGTCGAAGAGAACTTCTGGTAGGACTTCCGAAAAGGGTCATGGAATTTCGGGCTCCGAAGTGGACAAGGGACGAAAACGCAACAAATTAAGAAGAAAATCGCGAGACAGTTCGGATTCCGATGACAAGAAACGCGGGAGGAGCTCGAGAAGAAAGGGGAAATCCCGTAGACGATACAGTAGTGAGGAGGATTCTGATTCCGAGGATGCTGAATCTGAAAGCTCGGAGTATGACTCGGATTCGGAGAACTCCGACGCGGAATCTGCGTCGAGTAGTTCGGGTTCGGAAGGCGATAGCGAGAGCGAGGAGGAGAGGAGGAGGAGGAAGAGGAGGGAGAGGAGGAGGAGAAGAGAAAAGGAGGAAAGAGAGCGGAAGAGGAGGAAAAAGGAGAAGGAGAAGAAGAAGAGGAAAAAGGAGAGGGAGGAAGAGAAACGAAGGAAGGAGAAGAAAAAGGAGAAGAAGGAACGGGGAAAGAAGGGGGCAGTCACGAATTCGTGGGGAAAGTATGGGATTATCAAAGAAACTGATATGTGGTACGTGAAGATTCAATTCCATTAAATTTTCTGTTTGTTTACTGGGAAAATGTGGCGAGAGAGGAGATTATATGCTCGCACTGTCTTTATTTTTTGAAATTCAAGTCCTCTGCTTAGTCCTCCTTGTGCATGTAATCAACTGAACCATACTTTTGTAGATTTTCTATATTGGTTCATGCTTTCTTCTGCACAACCATTCACTCTCTCTTGCAATTTGTTCTTATTGACTATATTTTATAGGAACAAGCGACCAGAGTTCACTGCATGGTTGGCTGAAATCAAAAAGGTAAATTGATTTATGCGCAAAGTCTTCCATTATGGCTAATTAATGACATTTTTAAGTTGTAACTGTGTGGAATAGCTATATGCTATTGTTAAGTTACCTTCATTTTAAAGAGACTGCTAAATAGTTTCAATACAGTGGCCCCAACTTAATATTTCTTCAATATATGGAGTTGAATTGACTAAAACAAAATTAGTTTTTCATGTTACACAATCTAGGTAGCCCCCTTTTCTGTTTTTTGGATTCAAAGTACTTTAGTAGTGTACACCCGTGTTCGGGTCTTAGGGCTAGCGCTGGTGCCCCTCAGGGGAGTCGACTCCCAGTTATTTAAAAAAATTACTTTAGTAGTGTGCATATTCTTTTGATTGAACATAAAAGTCTACCAAGTTGAGACGAATTTACGATCTAATTGCTTTTGCAGTCGTTGTATTTTTGCTAATTATATTCTTTTTAATATTACATTGCTTAGGTGAACTTGGAAAGCCTAGCTAATTGGGAAGAGAAGCAAATGTTTAAGGAGTAAGTAGATGAATTGGTCATGTTTAGTTAACTCCTCACAGTCATTACCCTTGATGATAGCTGTTATATTTATTTATCTAATGACCACTTAATCTATTTTTGAAAGAACAACAAGAATTGTAAAGAGCTGCAATCTTACTCATCTTGTGATGATTGTTTATACAGATTCATGGAGGACCATAATACGGCCACTTTTCCATCTAAAAAGTAAGGTTCTGTGATTTTTGTTTGTGCAGCTAGGCTACTATGGTATTGTTTGAGAAGGTCGTAAGAAAGAAATGAACAAAGAACGCTAGATGGACCATTAATATGACCAATATTTCTTGGAGATTATTAAACTTTATACAATACCTTAAATCCAATCTTTATTTTTATCTCGAGTAAAAACTGAAGTTTTTCTTAAGATCTGCTCATCTACTCATGTTGTTGCTCGTGCGATTTAAGCCATCCATCATTAATCCGCATGCCTTTTTTTTTTTTTCCTGTCCTGTGATTTCAGGTACTACAACCTTGATGCTTATTACCAGCATAAAATGCTAAAAGATATGAAGAAGGGCCACAAAAAGCTTTCGGCAGGGGAACGCACTGTGTTTGATGATGAAGAACAGAGGAGGTATTTCTACTTTTCCTCTTATTTACCTGCTGTTCATTTTGAGGATTAAATGCACCCCATATTAAGAAATTTGCTCATCTGATGATTGAACATGCATGTTTGATGATGGAGCCTAACTACTCTTTTAGAATTGACAAAGAATTCTTTATTCAATCTTCAAAAGTATATCAAATCATTCATACAAGATCTTGAGAAATTTTGAACTAACTAAAAAAAAAAAGAAAACAATTACACCAAAAGCTGGGTCAATAAACAAACAAAGTATTACTAAAGGTAATTAACAAATTCTGTAACTCACTACATCATTCAGAGATGGTCAATTTGATGCAAACATTGTTTTGTTGGTTGGGTTTATAATTTCACTCTCCTTTTTCGTTCAGGCAAGAACTACTTCGAGAACGTGAAAAACACAAGGAAGAACAAGTGGAAGCCTTAAAGCGCTCTATGCAGACTGGAATGGTCAGTGATTTTACTCTC

mRNA sequence

GAAGAGAACGGAGCCCCGTCGAAGAGAACTTCTGGTAGGACTTCCGAAAAGGGTCATGGAATTTCGGGCTCCGAAGTGGACAAGGGACGAAAACGCAACAAATTAAGAAGAAAATCGCGAGACAGTTCGGATTCCGATGACAAGAAACGCGGGAGGAGCTCGAGAAGAAAGGGGAAATCCCGTAGACGATACAGTAGTGAGGAGGATTCTGATTCCGAGGATGCTGAATCTGAAAGCTCGGAGTATGACTCGGATTCGGAGAACTCCGACGCGGAATCTGCGTCGAGTAGTTCGGGTTCGGAAGGCGATAGCGAGAGCGAGGAGGAGAGGAGGAGGAGGAAGAGGAGGGAGAGGAGGAGGAGAAGAGAAAAGGAGGAAAGAGAGCGGAAGAGGAGGAAAAAGGAGAAGGAGAAGAAGAAGAGGAAAAAGGAGAGGGAGGAAGAGAAACGAAGGAAGGAGAAGAAAAAGGAGAAGAAGGAACGGGGAAAGAAGGGGGCAGTCACGAATTCGTGGGGAAAGTATGGGATTATCAAAGAAACTGATATGTGGAACAAGCGACCAGAGTTCACTGCATGGTTGGCTGAAATCAAAAAGGTGAACTTGGAAAGCCTAGCTAATTGGGAAGAGAAGCAAATGTTTAAGGAGTACTACAACCTTGATGCTTATTACCAGCATAAAATGCTAAAAGATATGAAGAAGGGCCACAAAAAGCTTTCGGCAGGGGAACGCACTGTGTTTGATGATGAAGAACAGAGGAGGCAAGAACTACTTCGAGAACGTGAAAAACACAAGGAAGAACAAGTGGAAGCCTTAAAGCGCTCTATGCAGACTGGAATGGTCAGTGATTTTACTCTC

Coding sequence (CDS)

GAAGAGAACGGAGCCCCGTCGAAGAGAACTTCTGGTAGGACTTCCGAAAAGGGTCATGGAATTTCGGGCTCCGAAGTGGACAAGGGACGAAAACGCAACAAATTAAGAAGAAAATCGCGAGACAGTTCGGATTCCGATGACAAGAAACGCGGGAGGAGCTCGAGAAGAAAGGGGAAATCCCGTAGACGATACAGTAGTGAGGAGGATTCTGATTCCGAGGATGCTGAATCTGAAAGCTCGGAGTATGACTCGGATTCGGAGAACTCCGACGCGGAATCTGCGTCGAGTAGTTCGGGTTCGGAAGGCGATAGCGAGAGCGAGGAGGAGAGGAGGAGGAGGAAGAGGAGGGAGAGGAGGAGGAGAAGAGAAAAGGAGGAAAGAGAGCGGAAGAGGAGGAAAAAGGAGAAGGAGAAGAAGAAGAGGAAAAAGGAGAGGGAGGAAGAGAAACGAAGGAAGGAGAAGAAAAAGGAGAAGAAGGAACGGGGAAAGAAGGGGGCAGTCACGAATTCGTGGGGAAAGTATGGGATTATCAAAGAAACTGATATGTGGAACAAGCGACCAGAGTTCACTGCATGGTTGGCTGAAATCAAAAAGGTGAACTTGGAAAGCCTAGCTAATTGGGAAGAGAAGCAAATGTTTAAGGAGTACTACAACCTTGATGCTTATTACCAGCATAAAATGCTAAAAGATATGAAGAAGGGCCACAAAAAGCTTTCGGCAGGGGAACGCACTGTGTTTGATGATGAAGAACAGAGGAGGCAAGAACTACTTCGAGAACGTGAAAAACACAAGGAAGAACAAGTGGAAGCCTTAAAGCGCTCTATGCAGACTGGAATGGTCAGTGATTTTACTCTC

Protein sequence

EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKSRRRYSSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEYYNLDAYYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGMVSDFTL

Homology

[Close]

BLAST of MS002723 vs. NCBI nr
Match: XP_022142930.1 (troponin I [Momordica charantia])

HSP 1 Score: 433.0 bits (1112), Expect = 2.1e-117
Identity = 277/293 (94.54%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS 60
           EENGAPSKRTSGRTSEKGHGISGSEVD+GRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS
Sbjct: 29  EENGAPSKRTSGRTSEKGHGISGSEVDEGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS 88

Query: 61  RRRYSSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR 120
           RRRYSSEEDSDSEDAE ESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR
Sbjct: 89  RRRYSSEEDSDSEDAEFESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR 148

Query: 121 RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET 180
           RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET
Sbjct: 149 RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET 208

Query: 181 DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDAYYQHK 240
           DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDAYYQHK
Sbjct: 209 DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDAYYQHK 268

Query: 241 MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM
Sbjct: 269 MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 321

[Close]

BLAST of MS002723 vs. NCBI nr
Match: XP_023515919.1 (vicilin-like seed storage protein At2g18540 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-92
Identity = 245/298 (82.21%), Postives = 261/298 (87.58%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+N APSKR SGR+         S+V++GRKR+K RRKSRDSSDSD   D+KRG+SS RK
Sbjct: 32  EDNEAPSKRNSGRS---------SDVEEGRKRSKSRRKSRDSSDSDEKHDRKRGKSSVRK 91

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSEDAESESSE DSDSE+SDAESAS+SSGSEG  ESEEERRRRKR+
Sbjct: 92  GKSRRRYSSSEEDSDSEDAESESSESDSDSEHSDAESASNSSGSEG--ESEEERRRRKRK 151

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRRRREKEERERKRR+KEKEKK+R+KE+EEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 152 ERRRRREKEERERKRRRKEKEKKRRRKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 211

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 212 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 271

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQHKMLKDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 272 YYQHKMLKDMKKGHKKILAGERTVFDDEEQRRQELLVEREKHKEEQVEALKRSMQTGM 318

[Close]

BLAST of MS002723 vs. NCBI nr
Match: KAG7031373.1 (hypothetical protein SDJN02_05413, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-92
Identity = 245/298 (82.21%), Postives = 261/298 (87.58%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+N APSKR SGR+         S+V++GRKR+K RRKSRDSSDSD   D+KRG+SS RK
Sbjct: 29  EDNEAPSKRNSGRS---------SDVEEGRKRSKSRRKSRDSSDSDEKHDRKRGKSSIRK 88

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSEDAESESSE DSDSE+SDAESAS+SSGSEG  ESEEERRRRKR+
Sbjct: 89  GKSRRRYSSSEEDSDSEDAESESSESDSDSEHSDAESASNSSGSEG--ESEEERRRRKRK 148

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRRRREKEERERKRR+KEKEKK+R+KE+EEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 149 ERRRRREKEERERKRRRKEKEKKRRRKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 208

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 209 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 268

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQHKMLKDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 269 YYQHKMLKDMKKGHKKILAGERTVFDDEEQRRQELLVEREKHKEEQVEALKRSMQTGM 315

[Close]

BLAST of MS002723 vs. NCBI nr
Match: KAG6600733.1 (hypothetical protein SDJN03_05966, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-92
Identity = 245/298 (82.21%), Postives = 261/298 (87.58%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+N APSKR SGR+         S+V++GRKR+K RRKSRDSSDSD   D+KRG+SS RK
Sbjct: 29  EDNEAPSKRNSGRS---------SDVEEGRKRSKSRRKSRDSSDSDEKHDRKRGKSSIRK 88

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSEDAESESSE DSDSE+SDAESAS+SSGSEG  ESEEERRRRKR+
Sbjct: 89  GKSRRRYSSSEEDSDSEDAESESSESDSDSEHSDAESASNSSGSEG--ESEEERRRRKRK 148

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRRRREKEERERKRR+KEKEKK+R+KE+EEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 149 ERRRRREKEERERKRRRKEKEKKRRRKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 208

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 209 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 268

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQHKMLKDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 269 YYQHKMLKDMKKGHKKILAGERTVFDDEEQRRQELLVEREKHKEEQVEALKRSMQTGM 315

[Close]

BLAST of MS002723 vs. NCBI nr
Match: XP_038906271.1 (vicilin-like seed storage protein At2g18540 [Benincasa hispida])

HSP 1 Score: 347.4 bits (890), Expect = 1.1e-91
Identity = 242/298 (81.21%), Postives = 259/298 (86.91%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+NGAPSK +SGR S+K    SGS+ D+GRKR K R KSRDS DSD   DKKRG+SSRRK
Sbjct: 27  EDNGAPSKISSGRNSDKDRENSGSDADEGRKRGKSRIKSRDSLDSDEKHDKKRGKSSRRK 86

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSED ES+SS YDSDS +SDAESAS+SSGSEGDS+SEEERRRRKR+
Sbjct: 87  GKSRRRYSSSEEDSDSEDTESDSSMYDSDSGHSDAESASNSSGSEGDSDSEEERRRRKRK 146

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRRRR+K ERERKRR+KEKEKK+R+KEREEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 147 ERRRRRDK-ERERKRRRKEKEKKRRRKEREEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 206

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 207 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 266

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQ K+ KDMKKGHKK+  GERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 267 YYQRKIQKDMKKGHKKIVEGERTVFDDEEQRRQELLIEREKHKEEQVEALKRSMQTGM 323

[Close]

BLAST of MS002723 vs. ExPASy TrEMBL
Match: A0A6J1CMB2 (troponin I OS=Momordica charantia OX=3673 GN=LOC111012923 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.0e-117
Identity = 277/293 (94.54%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS 60
           EENGAPSKRTSGRTSEKGHGISGSEVD+GRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS
Sbjct: 29  EENGAPSKRTSGRTSEKGHGISGSEVDEGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKS 88

Query: 61  RRRYSSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR 120
           RRRYSSEEDSDSEDAE ESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR
Sbjct: 89  RRRYSSEEDSDSEDAEFESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR 148

Query: 121 RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET 180
           RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET
Sbjct: 149 RREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKET 208

Query: 181 DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDAYYQHK 240
           DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDAYYQHK
Sbjct: 209 DMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDAYYQHK 268

Query: 241 MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM
Sbjct: 269 MLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 321

[Close]

BLAST of MS002723 vs. ExPASy TrEMBL
Match: A0A6J1KTR2 (troponin I OS=Cucurbita maxima OX=3661 GN=LOC111496293 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.2e-91
Identity = 243/298 (81.54%), Postives = 260/298 (87.25%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+N APSKR SGR+         S+V++GRKR+K RRKSRDSSDSD   D+KRG+S  RK
Sbjct: 32  EDNEAPSKRNSGRS---------SDVEEGRKRSKSRRKSRDSSDSDEKHDRKRGKSLIRK 91

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSEDAESESSE DSDSE+SDAESAS+SSGSEG  ESEEERRRRKR+
Sbjct: 92  GKSRRRYSSSEEDSDSEDAESESSESDSDSEHSDAESASNSSGSEG--ESEEERRRRKRK 151

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           E+RRRREKEERERKRR+KEKEKK+R+KE+EEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 152 EKRRRREKEERERKRRRKEKEKKRRRKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 211

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 212 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 271

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQHKMLKDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 272 YYQHKMLKDMKKGHKKILAGERTVFDDEEQRRQELLVEREKHKEEQVEALKRSMQTGM 318

[Close]

BLAST of MS002723 vs. ExPASy TrEMBL
Match: A0A6J1FXC4 (stress response protein NST1 OS=Cucurbita moschata OX=3662 GN=LOC111447918 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.6e-91
Identity = 243/298 (81.54%), Postives = 260/298 (87.25%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSD---DKKRGRSSRRK 60
           E+N APSKR S R+         S+V++GRKR+K RRKSRDSSDSD   D+KRG+SS RK
Sbjct: 29  EDNEAPSKRNSDRS---------SDVEEGRKRSKSRRKSRDSSDSDEKHDRKRGKSSVRK 88

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSEDAESESSE DSDSE+SDAESAS+SSGSEG  ESEEERRRRKR+
Sbjct: 89  GKSRRRYSSSEEDSDSEDAESESSESDSDSEHSDAESASNSSGSEG--ESEEERRRRKRK 148

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRR+REKEERERKRR+KEKEKK+R+KE+EEEKRRKE KKKEKKERGKKGAVTNSWGKYG
Sbjct: 149 ERRRKREKEERERKRRRKEKEKKRRRKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKYG 208

Query: 181 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDA 240
           IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDA
Sbjct: 209 IIKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDA 268

Query: 241 YYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YYQHKMLKDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 269 YYQHKMLKDMKKGHKKILAGERTVFDDEEQRRQELLVEREKHKEEQVEALKRSMQTGM 315

[Close]

BLAST of MS002723 vs. ExPASy TrEMBL
Match: A0A1S3BU92 (LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g18540 OS=Cucumis melo OX=3656 GN=LOC103493338 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 4.0e-90
Identity = 236/297 (79.46%), Postives = 257/297 (86.53%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSDD---KKRGRSSRRK 60
           E+N APSKR SG+ SEK    SGS+ D+GRKR K RRKSR++SDSD+   K+RGRSSRRK
Sbjct: 27  EDNSAPSKRISGKDSEKDRENSGSDADEGRKRTKSRRKSRENSDSDEKHGKRRGRSSRRK 86

Query: 61  GKSRRRY-SSEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRY SSEEDSDSED ES+SS YDS+S  SD+ESASSSSGSE DSESEEERRRRKR+
Sbjct: 87  GKSRRRYSSSEEDSDSEDTESDSSMYDSESGYSDSESASSSSGSEEDSESEEERRRRKRK 146

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGI 180
           ERRRRREK ERERKRR+KEKEKK+RKK  EE++R+++KKKEKKERGKKGAVTNSWGKYGI
Sbjct: 147 ERRRRREK-ERERKRRRKEKEKKRRKKREEEKRRKEKKKKEKKERGKKGAVTNSWGKYGI 206

Query: 181 IKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDAY 240
           IKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE              YYNLDAY
Sbjct: 207 IKETDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYNLDAY 266

Query: 241 YQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           YQ K+ KDMKKGHKK+ AGERTVFDDEEQRRQELL EREKHKEEQVEALKRSMQTGM
Sbjct: 267 YQRKIQKDMKKGHKKVVAGERTVFDDEEQRRQELLIEREKHKEEQVEALKRSMQTGM 322

[Close]

BLAST of MS002723 vs. ExPASy TrEMBL
Match: A0A0A0L2M5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G104880 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.6e-75
Identity = 221/301 (73.42%), Postives = 242/301 (80.40%), Query Frame = 0


Query: 1   EENGAPSKRTSGRTSEKGHGISGSEVDKGRKRNKLRRKSRDSSDSDD---KKRGRSSRRK 60
           E+N APSKR SG+ SEK    SG + D+GRKR K RRKSR++ DSD+   K+RGRSSRRK
Sbjct: 27  EDNSAPSKRISGKDSEKDRENSGPDADEGRKRTKSRRKSRETPDSDEKHGKRRGRSSRRK 86

Query: 61  GKSRRRYS-SEEDSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRR 120
           GKSRRRYS SEEDSDSE+ ES+SS YDSDS +SD+ESASSSSGSE DSESE ERR+RKR+
Sbjct: 87  GKSRRRYSTSEEDSDSENTESDSSMYDSDSGHSDSESASSSSGSEEDSESEGERRKRKRK 146

Query: 121 ERRRRREKEERERKRRKKEKEKKKRKKEREEEKRRKE-KKKEKKERGKKGAVTNSWGKYG 180
           ERRRRREK ERERKRR+KEKEKK+ KKE+EEEKRRKE KKKEKKERGKKGAVTNSWGK+G
Sbjct: 147 ERRRRREK-ERERKRRRKEKEKKRMKKEKEEEKRRKEKKKKEKKERGKKGAVTNSWGKFG 206

Query: 181 IIKETDMWNKRPEFTAWLAEIKK---VNLESLANWEEKQMFKE--------------YYN 240
           IIKETDMW    EF      +     VNLESLANWEEKQMFKE              YYN
Sbjct: 207 IIKETDMWYVTIEFHGCFCLLTAQILVNLESLANWEEKQMFKEFMEDHNTATFPSKKYYN 266

Query: 241 LDAYYQHKMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTG 280
           LDAYYQ K+ KDMKKG KK+ AGERTVFDDEEQRRQELL EREKHKEEQVE LKRSMQTG
Sbjct: 267 LDAYYQRKIQKDMKKGQKKVVAGERTVFDDEEQRRQELLIEREKHKEEQVEVLKRSMQTG 326

[Close]

BLAST of MS002723 vs. TAIR 10
Match: AT5G53800.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 168.3 bits (425), Expect = 8.8e-42
Identity = 153/294 (52.04%), Postives = 198/294 (67.35%), Query Frame = 0


Query: 8   KRTSGRTSEKGHG-ISGSEVDKGRKRNKLRRKSRDSSDSDDKKRGRSSRRKGKSRRRYSS 67
           +R S R    G G  SG E D  R+R   +R+++D +DS  +    S     K  RR S 
Sbjct: 23  RRKSSREKPSGSGKDSGEEKDVSRRRES-KRRTKDGNDSGSESGLESGSESEKEERRRSR 82

Query: 68  EE------DSDSEDAESESSEYDSDSENSDAESASSSSGSEGDSESEEERRRRKRRERRR 127
           ++      D  S  +     +Y S S +S++ES S  S SE +SESE+ERRRRKR  +R+
Sbjct: 83  KDRGKRKSDRKSSRSRRRRRDYSSSSSDSESESESEYSDSE-ESESEDERRRRKR--KRK 142

Query: 128 RREKEERERKRRKKEKEKKKR-KKEREEEKRRKEKKKEKKERGKKGAVTNSWGKYGIIKE 187
            RE+EE+ERKRR++EK+KKKR K +++ +K+RKEKKK+K E+ KKGAVT SWGKYGII+E
Sbjct: 143 EREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSEKVKKGAVTESWGKYGIIRE 202

Query: 188 TDMWNKRPEFTAWLAEIKKVNLESLANWEEKQMFKE--------------YYNLDAYYQH 247
           TDMWNKRPEFTAWL E+KKVNLESL  WEEK+MFK+              YY++D YY+ 
Sbjct: 203 TDMWNKRPEFTAWLLEVKKVNLESLPPWEEKKMFKDFMEDHNTGTFTSKKYYDIDGYYRL 262

Query: 248 KMLKDMKKGHKKLSAGERTVFDDEEQRRQELLREREKHKEEQVEALKRSMQTGM 280
           K+ K+MKKG KK    ERTVF+DEEQRR E+   RE+ KEE+V ALKRSM+ GM
Sbjct: 263 KLEKEMKKGLKKAGISERTVFNDEEQRRLEMQELRERQKEEEVLALKRSMEGGM 312

The following BLAST results are available for this feature:

BLAST of MS002723 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142930.1	2.1e-117	94.54	troponin I [Momordica charantia]	[more]
XP_023515919.1	2.3e-92	82.21	vicilin-like seed storage protein At2g18540 [Cucurbita pepo subsp. pepo]	[more]
KAG7031373.1	2.3e-92	82.21	hypothetical protein SDJN02_05413, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6600733.1	2.3e-92	82.21	hypothetical protein SDJN03_05966, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038906271.1	1.1e-91	81.21	vicilin-like seed storage protein At2g18540 [Benincasa hispida]	[more]

BLAST of MS002723 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS002723 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMB2	1.0e-117	94.54	troponin I OS=Momordica charantia OX=3673 GN=LOC111012923 PE=4 SV=1	[more]
A0A6J1KTR2	1.2e-91	81.54	troponin I OS=Cucurbita maxima OX=3661 GN=LOC111496293 PE=4 SV=1	[more]
A0A6J1FXC4	1.6e-91	81.54	stress response protein NST1 OS=Cucurbita moschata OX=3662 GN=LOC111447918 PE=4 ...	[more]
A0A1S3BU92	4.0e-90	79.46	LOW QUALITY PROTEIN: vicilin-like seed storage protein At2g18540 OS=Cucumis melo...	[more]
A0A0A0L2M5	1.6e-75	73.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G104880 PE=4 SV=1	[more]

BLAST of MS002723 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53800.1	8.8e-42	52.04	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 106..162
None	No IPR available	COILS	Coil	Coil	coord: 246..274
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 12..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 70..88
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 140..166
None	No IPR available	PANTHER	PTHR34689	NUCLEIC ACID-BINDING PROTEIN	coord: 24..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002723.1	MS002723.1	mRNA