MS002690 (gene) Bitter gourd (TR) v1

Overview


Name	MS002690
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold928: 314091 .. 317470 (-)
RNA-Seq Expression	MS002690
Synteny	MS002690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTTCTGTTGGGTGTCGCCGGCGGCGGTCGAACAGAGAGATACGCCATCGGTTGGGATGATTTGCATATCGATGCTCGCCGGCAGGGGCTGAACTCCGGCGACAACGGCGGCACTCGGATTATTGTGGTGGATAAAAATTGCTCCGGCGACTCGCCGACGGTCCAAGGGGCGGTGGATTTGGTTCCTCATTATAATAAACAGAGAGTCAAGATTTATATTCTTCCCGGAATTTACAGGTTCTTCGTATCATCAATTTACCCTTTTTTTTTCCTTTCTTTTGAATTTAGTCGGAAAAATGACCGGTTGAACCATGTTCATGAAGCCTCAATAACAAACATGGTTTCTTTTGATTTCTAATTTTTTTTTTTTTTGTAATGTTAAGATTATTGTATAATTTTGAGTATAGACTAAAGTTATTGTACAAGTTATAAATCATTCATGTAAAGATTTAAGTTATTGCGAACAATAAAAATTTTGTTATTGTTGTTACTGTGAAAAGAAAGGTGACTGTATATATTTCGAAATAAAAGTTATTTGTTAGGATGTATTGAATGAAAATATAAAATATATAGTTTCTTTTTACCTACATTCCTACTAAATTGTTTAAGAATGTCAAATGTGATTAGTTAACCTATTAGATGAAATTGAAAAATAATTTATTTAAATTTTTAGGTTATACAAGAAATAAAGTTTTCAGTTTTTGTTGACGTATTATAAAATCAAATTTTAGATTTTTTTTTAGTTCAACGAGGAGTAAAGAGCTAACCTTAACCTGAGTTTAGATATACGGCAATAACACATATGTCTCGAATCCTCCCATCCCACGGCAAAATTTAAGTCATTTGGATAAAAGTGATTGTCCATGCCAAAAATTAAGTTGTAGGTTAACATTTAATGAAACAAAATGAACGTCAAAAGTTTAAAAATTAAAAATTGTAGTAATAAAATATAGAAATATATAATTTCCTTATTTTTTTCTTTCTAATCGAATTGTGATGATTAGCCAGGTAAATAGTTACAAATTGTCATTAATAAATAAAATTGAATACATACCATTAAGTAATAGCTTAATATGTTTTTTTTTTGGGCTGTTTATTTTTTTTTATATGTGAATTACAATTTAAAATTATGTCTTTTATTATTTCATAAAATAATTATATGAGTTCATGCTTCTGAGTCATTGTCATCAACGATCTTTTCGGTTTATTACTTATTGGTTAGGAATACAGGCCCAAAATCGGCCCATAAAACCTAATGGGCCTATAATATTGTTGGGCCTTGATGTGCCTTACTTCTAAAAGCTAAGACCGAGGCCCAATTCATAGGCAGGGTTCGCTAGTCAAACTTTGATAGAATTTGGATCCTTAGTTTAGTTTTTTAGATAGTCATTTAAATTAATTTTTTAAGAAAATTGATTTTAAAATTCAAAAATTAAAATATATTAAAACTAATGATAGATGTTTTGAGTTAGACTTTAATATAAGTTTTTGTTTTTATATAAAGTTTGTTACTTTAATTATTTTGTTTAGTACAATGAATGTGGGGTATGGAATTGAACCTCCAACTTCAAGGAATTAGATGTCATTTAAATCTTTCCTACTCATTCTCGACCACATTGTTCTTATAGAGAGAGATGGTTGGGGAAAGAAAAGATTCTTTTTCTAGGATACCCTCGAGAAGAGATCTAATGGAGATGGAGTGGAACCTCTAGCTTAGAGGATTGAGCACATGGTTACGAACTATGGTGTCAAGAGTGGAACCTCTGGCAATTTTTACTTCTATTAAAACAATCATTTTTCTAAAAAATATTTTAATTATTAACTATTAGGAGAATGGGTTAAATAAGCTTCTAAACTTTTCCTTTTGCAGAGAGAAAGTAACTATACCAAGCACCAAGCCATATATTTCACTGATAGGAAACAAACGACGAGTCACGGACACCATCATTACATGGAACGACAAGGCATCCGATAGAGATATCAATGGAGGTGAACTCGGGACGTATAGAACAGCGACTGTTTCGGTCGAATCTGACTATTTTTGTGCAACAGGAATCACGTTCGAAGTAAGAATAATCATGTAGAAAACAAAGAGAGTACATAATGTTAGTTTGAAAGTTATCAAGTTACAAGAGTTGATTGTGGTTGTAGAATACAGTGGTTGCTGAGCCAGGAGACAGTGGAAGACAGGCAGTGGCTCTAAGAATTGCAGGAGATAAGGCAATGTTTTACAGAGTCAAATTCATAGGACAACAAGATACACTCTTGGATGACATAGGCACTCACTATTTCTACCAATGCCACATTCAAGGAAGCGTCGATTTCATATTTGGGAGAGCTAGATCGCTCTACGAGGTAATGAGTTCATAACACTTATCCCTCCCGAGTTTAACTTACCATTTTAATCTTTGTAGTTTGGCTTTGGTTTCAAACATGATCCCTATGATTTAAATTGTTTCTTTAGCCACCATTTCCCCCATTCTAACACGTGCAACTAATACTATCAACCAAATCAAATATAACAACAAGAAACAACTAATACCATCAACGGAACGAATATAACAACCTTTTGTCACGTAAATAGATTCTGTCCTAGCAATAGAACTAGTTCAGACATATATCTTCGACCAAAAGGTTCATGTTAAACTCAAAAGTAAAAAAAATGTCATACGTTCGATATGACAACAGTTTGTAACAGTTGAGATGATTTGTGAAATTTGACAAGACCTTGAGAACTAGAATGAAATATTAAAACCATGTTGACCAATTTGAAACCAATCCCAAACCAAAAGGATCCAAAATAAAACTAAGCAAAACTATACGGAACAAAATTGTTGATTTAACCCTTCCCTTCTTTTAAGCTTAGTAAATTCTTCATGTGATATTTTTATCTTCTCTTAAAATACCAGCAATGTATTATAAGTTCCACAGCTGAGAACTATGGAGCAATAGCAGCACATCATCGGGCCTCGGCAGAAGAAGAAACTGGGTTCTCTTTCGTACACTGCGTAATCAATGGAAGTGGCAAAATACTCCTGGGAAGAGCATGGGGAAACTATTCAAGAACAATATATTCTTACTGTTACATTCAAGATATAATAACTCCCACAGGATGGAGTGACTGGGGTGACCCATCAAGGCAAAGGTAAATAAATAACGAAGTACAGTAATGAGTAAAATTCCCCTACGAATCTAACTAATCTGATAGAATTTTCCAACTTTTTACCACACAGGACAGTAGTTTTTGGGCAGTATAATTGCAGAGGCAGTGGAGCTAACACACAAGGGTGGGTGCCATGGGCAAAGACTTTCAGCTATGAGGAAGTAAGACCTTTTCTAGATATGAAATTCATCAATGGAGAACAATGGCTTGACTTA

mRNA sequence

TTTGTTCTGTTGGGTGTCGCCGGCGGCGGTCGAACAGAGAGATACGCCATCGGTTGGGATGATTTGCATATCGATGCTCGCCGGCAGGGGCTGAACTCCGGCGACAACGGCGGCACTCGGATTATTGTGGTGGATAAAAATTGCTCCGGCGACTCGCCGACGGTCCAAGGGGCGGTGGATTTGGTTCCTCATTATAATAAACAGAGAGTCAAGATTTATATTCTTCCCGGAATTTACAGAGAGAAAGTAACTATACCAAGCACCAAGCCATATATTTCACTGATAGGAAACAAACGACGAGTCACGGACACCATCATTACATGGAACGACAAGGCATCCGATAGAGATATCAATGGAGGTGAACTCGGGACGTATAGAACAGCGACTGTTTCGGTCGAATCTGACTATTTTTGTGCAACAGGAATCACGTTCGAAAATACAGTGGTTGCTGAGCCAGGAGACAGTGGAAGACAGGCAGTGGCTCTAAGAATTGCAGGAGATAAGGCAATGTTTTACAGAGTCAAATTCATAGGACAACAAGATACACTCTTGGATGACATAGGCACTCACTATTTCTACCAATGCCACATTCAAGGAAGCGTCGATTTCATATTTGGGAGAGCTAGATCGCTCTACGAGCAATGTATTATAAGTTCCACAGCTGAGAACTATGGAGCAATAGCAGCACATCATCGGGCCTCGGCAGAAGAAGAAACTGGGTTCTCTTTCGTACACTGCGTAATCAATGGAAGTGGCAAAATACTCCTGGGAAGAGCATGGGGAAACTATTCAAGAACAATATATTCTTACTGTTACATTCAAGATATAATAACTCCCACAGGATGGAGTGACTGGGGTGACCCATCAAGGCAAAGGACAGTAGTTTTTGGGCAGTATAATTGCAGAGGCAGTGGAGCTAACACACAAGGGTGGGTGCCATGGGCAAAGACTTTCAGCTATGAGGAAGTAAGACCTTTTCTAGATATGAAATTCATCAATGGAGAACAATGGCTTGACTTA

Coding sequence (CDS)

TTTGTTCTGTTGGGTGTCGCCGGCGGCGGTCGAACAGAGAGATACGCCATCGGTTGGGATGATTTGCATATCGATGCTCGCCGGCAGGGGCTGAACTCCGGCGACAACGGCGGCACTCGGATTATTGTGGTGGATAAAAATTGCTCCGGCGACTCGCCGACGGTCCAAGGGGCGGTGGATTTGGTTCCTCATTATAATAAACAGAGAGTCAAGATTTATATTCTTCCCGGAATTTACAGAGAGAAAGTAACTATACCAAGCACCAAGCCATATATTTCACTGATAGGAAACAAACGACGAGTCACGGACACCATCATTACATGGAACGACAAGGCATCCGATAGAGATATCAATGGAGGTGAACTCGGGACGTATAGAACAGCGACTGTTTCGGTCGAATCTGACTATTTTTGTGCAACAGGAATCACGTTCGAAAATACAGTGGTTGCTGAGCCAGGAGACAGTGGAAGACAGGCAGTGGCTCTAAGAATTGCAGGAGATAAGGCAATGTTTTACAGAGTCAAATTCATAGGACAACAAGATACACTCTTGGATGACATAGGCACTCACTATTTCTACCAATGCCACATTCAAGGAAGCGTCGATTTCATATTTGGGAGAGCTAGATCGCTCTACGAGCAATGTATTATAAGTTCCACAGCTGAGAACTATGGAGCAATAGCAGCACATCATCGGGCCTCGGCAGAAGAAGAAACTGGGTTCTCTTTCGTACACTGCGTAATCAATGGAAGTGGCAAAATACTCCTGGGAAGAGCATGGGGAAACTATTCAAGAACAATATATTCTTACTGTTACATTCAAGATATAATAACTCCCACAGGATGGAGTGACTGGGGTGACCCATCAAGGCAAAGGACAGTAGTTTTTGGGCAGTATAATTGCAGAGGCAGTGGAGCTAACACACAAGGGTGGGTGCCATGGGCAAAGACTTTCAGCTATGAGGAAGTAAGACCTTTTCTAGATATGAAATTCATCAATGGAGAACAATGGCTTGACTTA

Protein sequence

FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL

Homology

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BLAST of MS002690 vs. NCBI nr
Match: XP_022139683.1 (pectinesterase QRT1 [Momordica charantia])

HSP 1 Score: 710.7 bits (1833), Expect = 6.1e-201
Identity = 339/340 (99.71%), Postives = 339/340 (99.71%), Query Frame = 0


Query: 1   FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD 60
           FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD
Sbjct: 19  FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD 78

Query: 61  LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG 120
           LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG
Sbjct: 79  LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG 138

Query: 121 ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ 180
           ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ
Sbjct: 139 ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ 198

Query: 181 DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEETG 240
           DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHR SAEEETG
Sbjct: 199 DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRDSAEEETG 258

Query: 241 FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN 300
           FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN
Sbjct: 259 FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN 318

Query: 301 CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL
Sbjct: 319 CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 358

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BLAST of MS002690 vs. NCBI nr
Match: XP_004150299.1 (pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_015463 [Cucumis sativus])

HSP 1 Score: 572.8 bits (1475), Expect = 2.0e-159
Identity = 273/345 (79.13%), Postives = 303/345 (87.83%), Query Frame = 0


Query: 1   FVLLGVAGG--GRTERYAIGWDDLHIDA-RRQGLNS--GDNGGTRIIVVDKNCSGDSPTV 60
           F+LL VAGG  GR     I WDDL +D  RRQ ++S  G   GTRIIVVDKNCSGDS TV
Sbjct: 15  FLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTV 74

Query: 61  QGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDR 120
           QGAVD+VPH NKQRVKIYILPGIYREKV IP TKPYISLIGNK RVTDT+ITWNDKASD+
Sbjct: 75  QGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDK 134

Query: 121 DINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVK 180
            I+G ELGTYRTATV+++SDYFCATGITFENTVVA+PGD GRQ VALRI GDKAMFYRVK
Sbjct: 135 SIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVK 194

Query: 181 FIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASA 240
           F+G QDTLLDD+GTHY+YQCHIQGSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHRAS 
Sbjct: 195 FLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASP 254

Query: 241 EEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVV 300
           +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQRTV 
Sbjct: 255 DDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVA 314

Query: 301 FGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           FGQYNCRGSGA+T+ WV WAKTF+YEEVRPF+D K+I GEQWL+L
Sbjct: 315 FGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 359

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BLAST of MS002690 vs. NCBI nr
Match: XP_008463039.1 (PREDICTED: pectinesterase QRT1 [Cucumis melo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 269/348 (77.30%), Postives = 303/348 (87.07%), Query Frame = 0


Query: 1   FVLLGVAGGGRTER----YAIGWDDLHIDA-RRQGLNSGD---NGGTRIIVVDKNCSGDS 60
           F+LL V  G  + R      I WDDL +D   RQ ++SG+     GTRIIVVDKNCSGDS
Sbjct: 31  FLLLCVVSGESSGRGRGGGVISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNCSGDS 90

Query: 61  PTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKA 120
            TVQGAVD+VPH NKQRVKIYILPGIYREK+ IP TKPYISLIGNK RVT+T+ITWNDKA
Sbjct: 91  TTVQGAVDMVPHNNKQRVKIYILPGIYREKLYIPITKPYISLIGNKNRVTETVITWNDKA 150

Query: 121 SDRDINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFY 180
           SD++ING ELGTY+TATV+++SDYFCATGITFENT VA+PGD GRQ VALRI GDKA+FY
Sbjct: 151 SDKNINGVELGTYKTATVAIDSDYFCATGITFENTNVAKPGDKGRQGVALRITGDKAVFY 210

Query: 181 RVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHR 240
           RVKF+G QDTLLDD+GTHYFYQCHI+GSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHR
Sbjct: 211 RVKFLGGQDTLLDDLGTHYFYQCHIRGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHR 270

Query: 241 ASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQR 300
           AS +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQR
Sbjct: 271 ASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQR 330

Query: 301 TVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           TVVFGQYNCRGSGANT+ WVPWAKTFSYEEVRPF+D K+I GEQWL+L
Sbjct: 331 TVVFGQYNCRGSGANTKDWVPWAKTFSYEEVRPFVDRKYIKGEQWLNL 378

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BLAST of MS002690 vs. NCBI nr
Match: XP_022975722.1 (pectinesterase QRT1 [Cucurbita maxima])

HSP 1 Score: 557.8 bits (1436), Expect = 6.6e-155
Identity = 262/342 (76.61%), Postives = 303/342 (88.60%), Query Frame = 0


Query: 7   AGGGRTERYAIGWDDLHIDA-RRQGLNSGDNG-------GTRIIVVDKNCSGDSPTVQGA 66
           AGGG  ERYAI WDDL +D   RQGL+SG +G       GTRIIVVD++ SGDS TVQGA
Sbjct: 36  AGGG--ERYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTGTRIIVVDRSSSGDSTTVQGA 95

Query: 67  VDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDIN 126
           VDLVPH N QRVKIYI PGIYREKV IP TKPYISLIGN++R+TDT+IT N KASD+D++
Sbjct: 96  VDLVPHNNNQRVKIYIRPGIYREKVYIPITKPYISLIGNRKRMTDTVITSNSKASDKDVS 155

Query: 127 GGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIG 186
           G ELGTYRTATV+++SDYFCA+GITFENTVV+ PGD GRQAVALRI+G+KAMFYRVKF+G
Sbjct: 156 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 215

Query: 187 QQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEE 246
           QQDTLLDD+GTHYFYQCHIQG++DFIFG ARSLYEQC+I+STAE +GAIAAHHR+S +++
Sbjct: 216 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPDDD 275

Query: 247 TGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQ 306
           TGFSFV CVINGSG++LLGRAWG YSRTIYSYCYI+D+I+P GWSDW DPSRQRT VFGQ
Sbjct: 276 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 335

Query: 307 YNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           Y CRGSGANT+GWVPWA+TF+ EEVRP++D  FINGEQWL+L
Sbjct: 336 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 375

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BLAST of MS002690 vs. NCBI nr
Match: XP_038896509.1 (pectinesterase QRT1 [Benincasa hispida])

HSP 1 Score: 557.0 bits (1434), Expect = 1.1e-154
Identity = 267/341 (78.30%), Postives = 296/341 (86.80%), Query Frame = 0


Query: 5   GVAGGGRTERY--AIGWDDLHID-ARRQGLNSGD--NGGTRIIVVDKNCSGDSPTVQGAV 64
           G  GGG TER    I WDDL +D +RRQG++SG+    GTRIIVVDKNCSG+S TVQGAV
Sbjct: 41  GGGGGGVTERSYGMISWDDLRVDVSRRQGISSGNELGNGTRIIVVDKNCSGNSTTVQGAV 100

Query: 65  DLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDING 124
           D+ PH NKQRVKIYILPGIYREKV IP TKPYISLIGNK RVTDT+ITWNDKASD+DING
Sbjct: 101 DMAPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTLITWNDKASDKDING 160

Query: 125 GELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQ 184
            ELGTYRTATV++++DYFCATGITFENTVVA+PGD GRQAVALRI GDKAMFYRVKF+G 
Sbjct: 161 VELGTYRTATVAIDADYFCATGITFENTVVAKPGDKGRQAVALRITGDKAMFYRVKFVGG 220

Query: 185 QDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEET 244
           QDTLLDD+GTHYFYQCHIQGSVDFIFG ARSLYEQC+I+STAE+ GAIAAHHRAS +++T
Sbjct: 221 QDTLLDDMGTHYFYQCHIQGSVDFIFGTARSLYEQCVITSTAESSGAIAAHHRASPDDDT 280

Query: 245 GFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQY 304
           GFSFV CVINGSGK+LLGRAWG YSR IYSYCYI+D+I P GW DW DPSRQRTVVFGQY
Sbjct: 281 GFSFVRCVINGSGKVLLGRAWGTYSRIIYSYCYIEDVIDPLGWGDWNDPSRQRTVVFGQY 340

Query: 305 NCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           NCRGSGAN +      K F YEEVRPF+D K+I GEQWL+L
Sbjct: 341 NCRGSGANNRRLGAMGKDFHYEEVRPFVDRKYIKGEQWLNL 381

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BLAST of MS002690 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 6.7e-126
Identity = 217/337 (64.39%), Postives = 259/337 (76.85%), Query Frame = 0


Query: 4   LGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVDLVP 63
           L V   GR ER     ++ +        N+      R+IVVDKN  GDS TVQGAVD+VP
Sbjct: 44  LRVVEDGRIERSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVP 103

Query: 64  HYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGGELG 123
             N QRVKI+ILPGIYREKV +P +KPYIS IGN+    DT+I+W+DKASD   +G ELG
Sbjct: 104 DSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELG 163

Query: 124 TYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQDTL 183
           TYRTA+VS+ESD+FCAT ITFENTVVAE G+ GRQAVALRI GDKA+FYRV+ +G QDTL
Sbjct: 164 TYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTL 223

Query: 184 LDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEETGFSF 243
            DD G+HYFYQC+IQG+VDFIFG A+SLY+ C I STA+ YGAIAAHHR S  E+TGFSF
Sbjct: 224 FDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSF 283

Query: 244 VHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYNCRG 303
           V+C I+G+G+I LGRAWGNYSRT+YS C+I DIITP GWSDW  P RQR V+FG+YNCRG
Sbjct: 284 VNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRG 343

Query: 304 SGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
            GA   G VPW+KT + +EV+PFL  +FI G+QWL L
Sbjct: 344 RGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

[Close]

BLAST of MS002690 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-79
Identity = 145/303 (47.85%), Postives = 189/303 (62.38%), Query Frame = 0


Query: 38  GTRIIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGN 97
           G ++I V  N      +VQ AVD +P  N + + I I PG YREKV +P+TKPYI+  G 
Sbjct: 57  GHKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGA 116

Query: 98  KRRVTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTVVAE-PGDSG 157
            R V  T I W+D+ASD   NG +L TY+TA+V+V ++YF A  I+F NT  A  PG  G
Sbjct: 117 GRDV--TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQG 176

Query: 158 RQAVALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCI 217
            QAVA RI+GDKA F    F G QDTL DD G HYF +C+I+GS+DFIFG  RS+Y+ C 
Sbjct: 177 WQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 236

Query: 218 ISSTAENYGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDI 277
           + S A  +G+IAAH R   EE+TGF+FV C + G+G + +GRA G YSR +Y+Y Y   +
Sbjct: 237 LHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDAL 296

Query: 278 ITPTGWSDWGDPS-RQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGE 337
           +   GW DW   S + +T  FG YNC G GA     V WA+   YE   PF+   F+NG 
Sbjct: 297 VAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGR 356

Query: 338 QWL 339
            W+
Sbjct: 357 HWI 357

[Close]

BLAST of MS002690 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.1e-79
Identity = 140/301 (46.51%), Postives = 194/301 (64.45%), Query Frame = 0


Query: 41  IIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRR 100
           + V  K+  GD   +Q A+D +P  N  RV I +  G+Y+EKV+IP  K +I++ G    
Sbjct: 85  LTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEG--EG 144

Query: 101 VTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPGDSGRQA 160
              T + W D A   D  G  +GTY +A+ +V S +F A  ITF NT  V  PG  G+QA
Sbjct: 145 AEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQA 204

Query: 161 VALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISS 220
           VALR++ D A F+  + +G QDTL D +G HY+  C+I+GSVDFIFG A SLYE C + +
Sbjct: 205 VALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHA 264

Query: 221 TAENYGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITP 280
            A+  GA+ A  R+S  E+TGFSFV C + G+G + LGRAWG +SR +++Y Y+ +II P
Sbjct: 265 IADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILP 324

Query: 281 TGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLD 340
            GW +WGDPSR+ TV +GQY C G+GAN  G V WA+  + EE +PFL + FI+G +W+ 
Sbjct: 325 RGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 383

[Close]

BLAST of MS002690 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-68
Identity = 138/313 (44.09%), Postives = 188/313 (60.06%), Query Frame = 0


Query: 35  DNGGTRIIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISL 94
           D   T  + VDKN   +  TVQ AVD V +++++R  I+I  G+Y EKV IP TKP I+L
Sbjct: 84  DTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITL 143

Query: 95  IGNKRRVTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPG 154
            G    +  T I WND A   +      GT+  ATV V    F A  I+F N   + +PG
Sbjct: 144 QGQGFDI--TAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPG 203

Query: 155 DSGRQAVALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYE 214
           D G QAVA+RIAGD++ F    F G QDTL DD G HYF  C+IQGS+DFIFG A+SLY+
Sbjct: 204 DVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQ 263

Query: 215 QCIISSTAENY--------GAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSR 274
            C I S A           GA+ A+ R+S +E +GFSFV+C I G+G + LGRAW  YSR
Sbjct: 264 DCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSR 323

Query: 275 TIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRP 334
            ++    + D+I P GW+++ DPSR  T+ +G+YNC G GA+     P+ +  +  +V  
Sbjct: 324 VVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVAL 383

Query: 335 FLDMKFINGEQWL 339
            ++  FI+G+QWL
Sbjct: 384 LINTSFIDGDQWL 388

[Close]

BLAST of MS002690 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.8e-66
Identity = 131/304 (43.09%), Postives = 187/304 (61.51%), Query Frame = 0


Query: 41  IIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRR 100
           ++ VD +  G+   VQ A+D+VP  +  +  I +  G YREKVT+   K  + + G  R 
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG--RG 149

Query: 101 VTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPGDSGRQA 160
             +T I WND A           T  + +  V +  F A  I+F+N     +PG++  QA
Sbjct: 150 YQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQA 209

Query: 161 VALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISS 220
           VALRI GD+A FY   F G QDTLLDD G H+F +C IQGS+DFIFG  RSLY+ C I+S
Sbjct: 210 VALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINS 269

Query: 221 TAEN-----YGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQ 280
            A+       G+I A  R S +E++GFSFV+C I+GSG+ILLGRAWG Y+  ++S  Y+ 
Sbjct: 270 IAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMS 329

Query: 281 DIITPTGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFING 339
            IITP GW++WGD ++++TV FG++ C G GA+ +  V + K  +  E   F+D+ FI+G
Sbjct: 330 GIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDG 385

[Close]

BLAST of MS002690 vs. ExPASy TrEMBL
Match: A0A6J1CG92 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.0e-201
Identity = 339/340 (99.71%), Postives = 339/340 (99.71%), Query Frame = 0


Query: 1   FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD 60
           FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD
Sbjct: 19  FVLLGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVD 78

Query: 61  LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG 120
           LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG
Sbjct: 79  LVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGG 138

Query: 121 ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ 180
           ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ
Sbjct: 139 ELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQ 198

Query: 181 DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEETG 240
           DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHR SAEEETG
Sbjct: 199 DTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRDSAEEETG 258

Query: 241 FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN 300
           FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN
Sbjct: 259 FSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYN 318

Query: 301 CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL
Sbjct: 319 CRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 358

[Close]

BLAST of MS002690 vs. ExPASy TrEMBL
Match: A0A0A0K706 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.7e-160
Identity = 273/345 (79.13%), Postives = 303/345 (87.83%), Query Frame = 0


Query: 1   FVLLGVAGG--GRTERYAIGWDDLHIDA-RRQGLNS--GDNGGTRIIVVDKNCSGDSPTV 60
           F+LL VAGG  GR     I WDDL +D  RRQ ++S  G   GTRIIVVDKNCSGDS TV
Sbjct: 26  FLLLCVAGGSSGRGRGGVISWDDLRVDVWRRQRMSSSEGLGNGTRIIVVDKNCSGDSTTV 85

Query: 61  QGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDR 120
           QGAVD+VPH NKQRVKIYILPGIYREKV IP TKPYISLIGNK RVTDT+ITWNDKASD+
Sbjct: 86  QGAVDMVPHNNKQRVKIYILPGIYREKVYIPITKPYISLIGNKNRVTDTVITWNDKASDK 145

Query: 121 DINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVK 180
            I+G ELGTYRTATV+++SDYFCATGITFENTVVA+PGD GRQ VALRI GDKAMFYRVK
Sbjct: 146 SIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGVALRITGDKAMFYRVK 205

Query: 181 FIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASA 240
           F+G QDTLLDD+GTHY+YQCHIQGSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHRAS 
Sbjct: 206 FLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHRASP 265

Query: 241 EEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVV 300
           +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQRTV 
Sbjct: 266 DDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQRTVA 325

Query: 301 FGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           FGQYNCRGSGA+T+ WV WAKTF+YEEVRPF+D K+I GEQWL+L
Sbjct: 326 FGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 370

[Close]

BLAST of MS002690 vs. ExPASy TrEMBL
Match: A0A1S3CIB1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.9e-158
Identity = 269/348 (77.30%), Postives = 303/348 (87.07%), Query Frame = 0


Query: 1   FVLLGVAGGGRTER----YAIGWDDLHIDA-RRQGLNSGD---NGGTRIIVVDKNCSGDS 60
           F+LL V  G  + R      I WDDL +D   RQ ++SG+     GTRIIVVDKNCSGDS
Sbjct: 31  FLLLCVVSGESSGRGRGGGVISWDDLRVDVWLRQRMSSGEGLGRNGTRIIVVDKNCSGDS 90

Query: 61  PTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKA 120
            TVQGAVD+VPH NKQRVKIYILPGIYREK+ IP TKPYISLIGNK RVT+T+ITWNDKA
Sbjct: 91  TTVQGAVDMVPHNNKQRVKIYILPGIYREKLYIPITKPYISLIGNKNRVTETVITWNDKA 150

Query: 121 SDRDINGGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFY 180
           SD++ING ELGTY+TATV+++SDYFCATGITFENT VA+PGD GRQ VALRI GDKA+FY
Sbjct: 151 SDKNINGVELGTYKTATVAIDSDYFCATGITFENTNVAKPGDKGRQGVALRITGDKAVFY 210

Query: 181 RVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHR 240
           RVKF+G QDTLLDD+GTHYFYQCHI+GSVDFIFG ARSLYEQC+I+STAE+YGAIAAHHR
Sbjct: 211 RVKFLGGQDTLLDDLGTHYFYQCHIRGSVDFIFGTARSLYEQCVITSTAESYGAIAAHHR 270

Query: 241 ASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQR 300
           AS +++TGFSFV CVINGSGK+ LGRAWGNYSRTIYS CYI+D+I P GWSDW DPSRQR
Sbjct: 271 ASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPLGWSDWNDPSRQR 330

Query: 301 TVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           TVVFGQYNCRGSGANT+ WVPWAKTFSYEEVRPF+D K+I GEQWL+L
Sbjct: 331 TVVFGQYNCRGSGANTKDWVPWAKTFSYEEVRPFVDRKYIKGEQWLNL 378

[Close]

BLAST of MS002690 vs. ExPASy TrEMBL
Match: A0A6J1IDU4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 3.2e-155
Identity = 262/342 (76.61%), Postives = 303/342 (88.60%), Query Frame = 0


Query: 7   AGGGRTERYAIGWDDLHIDA-RRQGLNSGDNG-------GTRIIVVDKNCSGDSPTVQGA 66
           AGGG  ERYAI WDDL +D   RQGL+SG +G       GTRIIVVD++ SGDS TVQGA
Sbjct: 36  AGGG--ERYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTGTRIIVVDRSSSGDSTTVQGA 95

Query: 67  VDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDIN 126
           VDLVPH N QRVKIYI PGIYREKV IP TKPYISLIGN++R+TDT+IT N KASD+D++
Sbjct: 96  VDLVPHNNNQRVKIYIRPGIYREKVYIPITKPYISLIGNRKRMTDTVITSNSKASDKDVS 155

Query: 127 GGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIG 186
           G ELGTYRTATV+++SDYFCA+GITFENTVV+ PGD GRQAVALRI+G+KAMFYRVKF+G
Sbjct: 156 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 215

Query: 187 QQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEE 246
           QQDTLLDD+GTHYFYQCHIQG++DFIFG ARSLYEQC+I+STAE +GAIAAHHR+S +++
Sbjct: 216 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPDDD 275

Query: 247 TGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQ 306
           TGFSFV CVINGSG++LLGRAWG YSRTIYSYCYI+D+I+P GWSDW DPSRQRT VFGQ
Sbjct: 276 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 335

Query: 307 YNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           Y CRGSGANT+GWVPWA+TF+ EEVRP++D  FINGEQWL+L
Sbjct: 336 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 375

[Close]

BLAST of MS002690 vs. ExPASy TrEMBL
Match: A0A6J1F7U7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 261/342 (76.32%), Postives = 301/342 (88.01%), Query Frame = 0


Query: 7   AGGGRTERYAIGWDDLHIDA-RRQGLNSGDNGG-------TRIIVVDKNCSGDSPTVQGA 66
           AGGG  +RYAI WDDL +D   RQGL+SG +GG       TRIIVVDK  SGDS TVQGA
Sbjct: 34  AGGG--DRYAISWDDLRVDVPSRQGLSSGVHGGGVQNGTRTRIIVVDKTSSGDSTTVQGA 93

Query: 67  VDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDIN 126
           VDLVPH N  RVKIYI PGIYREKV IP TKPYISLIGN++R+TDT+IT N KASD+D++
Sbjct: 94  VDLVPHNNNLRVKIYIRPGIYREKVYIPITKPYISLIGNRKRMTDTVITSNSKASDKDVS 153

Query: 127 GGELGTYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIG 186
           G ELGTYRTATV+++SDYFCA+GITFENTVV+ PGD GRQAVALRI+G+KAMFYRVKF+G
Sbjct: 154 GLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAVALRISGEKAMFYRVKFLG 213

Query: 187 QQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEE 246
           QQDTLLDD+GTHYFYQCHIQG++DFIFG ARSLYEQC+I+STAE +GAIAAHHR+S +++
Sbjct: 214 QQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITSTAETFGAIAAHHRSSPDDD 273

Query: 247 TGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQ 306
           TGFSFV CVINGSG++LLGRAWG YSRTIYSYCYI+D+I+P GWSDW DPSRQRT VFGQ
Sbjct: 274 TGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQ 333

Query: 307 YNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
           Y CRGSGANT+GWVPWA+TF+ EEVRP++D  FINGEQWL+L
Sbjct: 334 YRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 373

[Close]

BLAST of MS002690 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 4.8e-127
Identity = 217/337 (64.39%), Postives = 259/337 (76.85%), Query Frame = 0


Query: 4   LGVAGGGRTERYAIGWDDLHIDARRQGLNSGDNGGTRIIVVDKNCSGDSPTVQGAVDLVP 63
           L V   GR ER     ++ +        N+      R+IVVDKN  GDS TVQGAVD+VP
Sbjct: 44  LRVVEDGRIERSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVP 103

Query: 64  HYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRRVTDTIITWNDKASDRDINGGELG 123
             N QRVKI+ILPGIYREKV +P +KPYIS IGN+    DT+I+W+DKASD   +G ELG
Sbjct: 104 DSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELG 163

Query: 124 TYRTATVSVESDYFCATGITFENTVVAEPGDSGRQAVALRIAGDKAMFYRVKFIGQQDTL 183
           TYRTA+VS+ESD+FCAT ITFENTVVAE G+ GRQAVALRI GDKA+FYRV+ +G QDTL
Sbjct: 164 TYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTL 223

Query: 184 LDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISSTAENYGAIAAHHRASAEEETGFSF 243
            DD G+HYFYQC+IQG+VDFIFG A+SLY+ C I STA+ YGAIAAHHR S  E+TGFSF
Sbjct: 224 FDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSF 283

Query: 244 VHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYNCRG 303
           V+C I+G+G+I LGRAWGNYSRT+YS C+I DIITP GWSDW  P RQR V+FG+YNCRG
Sbjct: 284 VNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRG 343

Query: 304 SGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLDL 341
            GA   G VPW+KT + +EV+PFL  +FI G+QWL L
Sbjct: 344 RGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

[Close]

BLAST of MS002690 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.6e-80
Identity = 145/303 (47.85%), Postives = 189/303 (62.38%), Query Frame = 0


Query: 38  GTRIIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGN 97
           G ++I V  N      +VQ AVD +P  N + + I I PG YREKV +P+TKPYI+  G 
Sbjct: 57  GHKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGA 116

Query: 98  KRRVTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTVVAE-PGDSG 157
            R V  T I W+D+ASD   NG +L TY+TA+V+V ++YF A  I+F NT  A  PG  G
Sbjct: 117 GRDV--TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQG 176

Query: 158 RQAVALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCI 217
            QAVA RI+GDKA F    F G QDTL DD G HYF +C+I+GS+DFIFG  RS+Y+ C 
Sbjct: 177 WQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCE 236

Query: 218 ISSTAENYGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDI 277
           + S A  +G+IAAH R   EE+TGF+FV C + G+G + +GRA G YSR +Y+Y Y   +
Sbjct: 237 LHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDAL 296

Query: 278 ITPTGWSDWGDPS-RQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGE 337
           +   GW DW   S + +T  FG YNC G GA     V WA+   YE   PF+   F+NG 
Sbjct: 297 VAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGR 356

Query: 338 QWL 339
            W+
Sbjct: 357 HWI 357

[Close]

BLAST of MS002690 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.7e-80
Identity = 140/301 (46.51%), Postives = 194/301 (64.45%), Query Frame = 0


Query: 41  IIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRR 100
           + V  K+  GD   +Q A+D +P  N  RV I +  G+Y+EKV+IP  K +I++ G    
Sbjct: 85  LTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEG--EG 144

Query: 101 VTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPGDSGRQA 160
              T + W D A   D  G  +GTY +A+ +V S +F A  ITF NT  V  PG  G+QA
Sbjct: 145 AEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQA 204

Query: 161 VALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISS 220
           VALR++ D A F+  + +G QDTL D +G HY+  C+I+GSVDFIFG A SLYE C + +
Sbjct: 205 VALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHA 264

Query: 221 TAENYGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQDIITP 280
            A+  GA+ A  R+S  E+TGFSFV C + G+G + LGRAWG +SR +++Y Y+ +II P
Sbjct: 265 IADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILP 324

Query: 281 TGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFINGEQWLD 340
            GW +WGDPSR+ TV +GQY C G+GAN  G V WA+  + EE +PFL + FI+G +W+ 
Sbjct: 325 RGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 383

[Close]

BLAST of MS002690 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.7e-69
Identity = 138/313 (44.09%), Postives = 188/313 (60.06%), Query Frame = 0


Query: 35  DNGGTRIIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISL 94
           D   T  + VDKN   +  TVQ AVD V +++++R  I+I  G+Y EKV IP TKP I+L
Sbjct: 84  DTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITL 143

Query: 95  IGNKRRVTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPG 154
            G    +  T I WND A   +      GT+  ATV V    F A  I+F N   + +PG
Sbjct: 144 QGQGFDI--TAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPG 203

Query: 155 DSGRQAVALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYE 214
           D G QAVA+RIAGD++ F    F G QDTL DD G HYF  C+IQGS+DFIFG A+SLY+
Sbjct: 204 DVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQ 263

Query: 215 QCIISSTAENY--------GAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSR 274
            C I S A           GA+ A+ R+S +E +GFSFV+C I G+G + LGRAW  YSR
Sbjct: 264 DCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSR 323

Query: 275 TIYSYCYIQDIITPTGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRP 334
            ++    + D+I P GW+++ DPSR  T+ +G+YNC G GA+     P+ +  +  +V  
Sbjct: 324 VVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVAL 383

Query: 335 FLDMKFINGEQWL 339
            ++  FI+G+QWL
Sbjct: 384 LINTSFIDGDQWL 388

[Close]

BLAST of MS002690 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.6e-67
Identity = 131/304 (43.09%), Postives = 187/304 (61.51%), Query Frame = 0


Query: 41  IIVVDKNCSGDSPTVQGAVDLVPHYNKQRVKIYILPGIYREKVTIPSTKPYISLIGNKRR 100
           ++ VD +  G+   VQ A+D+VP  +  +  I +  G YREKVT+   K  + + G  R 
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG--RG 149

Query: 101 VTDTIITWNDKASDRDINGGELGTYRTATVSVESDYFCATGITFENTV-VAEPGDSGRQA 160
             +T I WND A           T  + +  V +  F A  I+F+N     +PG++  QA
Sbjct: 150 YQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQA 209

Query: 161 VALRIAGDKAMFYRVKFIGQQDTLLDDIGTHYFYQCHIQGSVDFIFGRARSLYEQCIISS 220
           VALRI GD+A FY   F G QDTLLDD G H+F +C IQGS+DFIFG  RSLY+ C I+S
Sbjct: 210 VALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINS 269

Query: 221 TAEN-----YGAIAAHHRASAEEETGFSFVHCVINGSGKILLGRAWGNYSRTIYSYCYIQ 280
            A+       G+I A  R S +E++GFSFV+C I+GSG+ILLGRAWG Y+  ++S  Y+ 
Sbjct: 270 IAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMS 329

Query: 281 DIITPTGWSDWGDPSRQRTVVFGQYNCRGSGANTQGWVPWAKTFSYEEVRPFLDMKFING 339
            IITP GW++WGD ++++TV FG++ C G GA+ +  V + K  +  E   F+D+ FI+G
Sbjct: 330 GIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDG 385

The following BLAST results are available for this feature:

BLAST of MS002690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139683.1	6.1e-201	99.71	pectinesterase QRT1 [Momordica charantia]	[more]
XP_004150299.1	2.0e-159	79.13	pectinesterase QRT1 [Cucumis sativus] >KGN44235.2 hypothetical protein Csa_01546...	[more]
XP_008463039.1	1.4e-157	77.30	PREDICTED: pectinesterase QRT1 [Cucumis melo]	[more]
XP_022975722.1	6.6e-155	76.61	pectinesterase QRT1 [Cucurbita maxima]	[more]
XP_038896509.1	1.1e-154	78.30	pectinesterase QRT1 [Benincasa hispida]	[more]

BLAST of MS002690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FM79	6.7e-126	64.39	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8LPF3	3.6e-79	47.85	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q8VYZ3	8.1e-79	46.51	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
O23038	3.8e-68	44.09	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	7.8e-66	43.09	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of MS002690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CG92	3.0e-201	99.71	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010530 PE=4 SV=1	[more]
A0A0A0K706	9.7e-160	79.13	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1	[more]
A0A1S3CIB1	6.9e-158	77.30	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1	[more]
A0A6J1IDU4	3.2e-155	76.61	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475843 PE=4 SV=1	[more]
A0A6J1F7U7	2.7e-154	76.32	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111442894 PE=4 SV=1	[more]

BLAST of MS002690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55590.1	4.8e-127	64.39	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	2.6e-80	47.85	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	5.7e-80	46.51	Pectin lyase-like superfamily protein	[more]
AT1G05310.1	2.7e-69	44.09	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	5.6e-67	43.09	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 42..334 e-value: 1.0E-62 score: 211.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 38..340 e-value: 5.8E-101 score: 340.0
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 13..339
None	No IPR available	PANTHER	PTHR31321:SF31	PECTINESTERASE QRT1	coord: 13..339
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 197..206
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 40..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002690.1	MS002690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity