MS002682 (gene) Bitter gourd (TR) v1

Overview


Name	MS002682
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	two-pore potassium channel 1
Location	scaffold928: 261053 .. 262247 (-)
RNA-Seq Expression	MS002682
Synteny	MS002682

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAGCAAGGAAGCAAGACAGCCACTGCTATCAACGTCGTCTAATACTTCTAGAACAAGGTTAATAGATATTCCTAGAAGCAAAAGACGACTGAGGCGCTCTAAGAGTGCTCCTCATGGTGATTACGCTCTTCCAGAGACCACCAGCACGTGCACTGGCACAGCCACTGGTTCAGTTCCACGTTCAGGATTTGTTTTTGGAAATCTACACCCGAGTCTTAGAAGGGTAGCCATAGTTTTAACAGTCTACTTGGGTATAGGAACTTTATGTTTTTACCTTGCCAGGAACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTCACAATTGTGACAATGACAACCGTTGGATACGGAGATCTTGTCCCGAACAGTCCCGGTGCAAAGCTACTGGCTTGTTCTTTTGTTTTCACAGGAATGGCTCTTATTGGTCTAATACTAAGCAATGCAGCTGACTATTTGGTAGAGAAGCAGGAAATCATGCTATTTAATGCCTTGCATACGTACCAAAATCCTGGCCCTTGTGACACCACAAAAGAATATGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCCTTCTTCTTTTCTTCATCATTTCGGGGAATACCTTACTAGTTACCCTTGAGAAGTTAGATCTCATTGATGCATTTTATTGTGTATGTTCTACCATCACCACCCTAGGCTATGGTGATAAAAGCTTCTCAACCAGGTGGGGGCGTGTGTTCGCCATTTTCTGGATTTTGATAAGTACCATCACTCTGGCACAATTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGACAAAGGTCATTGGTAAAGTGGGTTCTAAGTAGAAAGGTGACCGACATAGACCTCGAGGCAGCGGATATTGATGACGACGGGGTCGTCGGGTAAGTCTCAAGCAATTCTCAACTGTATTCCTGTTTGTATGATCTTCATTGTTGTTGTTACTAATACATTGACTAGTTGAGTTACATTCAAGTCGACACTTATTGTTTCGTTTTTACTCTTCCACAGGGCTGCAGAATTTGTCATATACAAGCTGAAAGAGATGGGGAAAATTACTGAAGATGACATTTCACCTGTGCTGAAGGAGTTCGAGGATCTTGATTTTGATCAATCAGGAACCTTGTCCATATCCGATATAACACTTGCCCAATTGTCT

mRNA sequence

ATGGCTAGCAAGGAAGCAAGACAGCCACTGCTATCAACGTCGTCTAATACTTCTAGAACAAGGTTAATAGATATTCCTAGAAGCAAAAGACGACTGAGGCGCTCTAAGAGTGCTCCTCATGGTGATTACGCTCTTCCAGAGACCACCAGCACGTGCACTGGCACAGCCACTGGTTCAGTTCCACGTTCAGGATTTGTTTTTGGAAATCTACACCCGAGTCTTAGAAGGGTAGCCATAGTTTTAACAGTCTACTTGGGTATAGGAACTTTATGTTTTTACCTTGCCAGGAACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTCACAATTGTGACAATGACAACCGTTGGATACGGAGATCTTGTCCCGAACAGTCCCGGTGCAAAGCTACTGGCTTGTTCTTTTGTTTTCACAGGAATGGCTCTTATTGGTCTAATACTAAGCAATGCAGCTGACTATTTGGTAGAGAAGCAGGAAATCATGCTATTTAATGCCTTGCATACGTACCAAAATCCTGGCCCTTGTGACACCACAAAAGAATATGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCCTTCTTCTTTTCTTCATCATTTCGGGGAATACCTTACTAGTTACCCTTGAGAAGTTAGATCTCATTGATGCATTTTATTGTGTATGTTCTACCATCACCACCCTAGGCTATGGTGATAAAAGCTTCTCAACCAGGTGGGGGCGTGTGTTCGCCATTTTCTGGATTTTGATAAGTACCATCACTCTGGCACAATTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGACAAAGGTCATTGGTAAAGTGGGTTCTAAGTAGAAAGGTGACCGACATAGACCTCGAGGCAGCGGATATTGATGACGACGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTGAAAGAGATGGGGAAAATTACTGAAGATGACATTTCACCTGTGCTGAAGGAGTTCGAGGATCTTGATTTTGATCAATCAGGAACCTTGTCCATATCCGATATAACACTTGCCCAATTGTCT

Coding sequence (CDS)

ATGGCTAGCAAGGAAGCAAGACAGCCACTGCTATCAACGTCGTCTAATACTTCTAGAACAAGGTTAATAGATATTCCTAGAAGCAAAAGACGACTGAGGCGCTCTAAGAGTGCTCCTCATGGTGATTACGCTCTTCCAGAGACCACCAGCACGTGCACTGGCACAGCCACTGGTTCAGTTCCACGTTCAGGATTTGTTTTTGGAAATCTACACCCGAGTCTTAGAAGGGTAGCCATAGTTTTAACAGTCTACTTGGGTATAGGAACTTTATGTTTTTACCTTGCCAGGAACCAAATCAAAGGGGAAAAAACAAACGGAATAGTTGATGCCATTTATTTCACAATTGTGACAATGACAACCGTTGGATACGGAGATCTTGTCCCGAACAGTCCCGGTGCAAAGCTACTGGCTTGTTCTTTTGTTTTCACAGGAATGGCTCTTATTGGTCTAATACTAAGCAATGCAGCTGACTATTTGGTAGAGAAGCAGGAAATCATGCTATTTAATGCCTTGCATACGTACCAAAATCCTGGCCCTTGTGACACCACAAAAGAATATGATACTAATAAAGCTAGAAACAAGTGTATAGTGGTGTTTCTCCTTCTTCTTTTCTTCATCATTTCGGGGAATACCTTACTAGTTACCCTTGAGAAGTTAGATCTCATTGATGCATTTTATTGTGTATGTTCTACCATCACCACCCTAGGCTATGGTGATAAAAGCTTCTCAACCAGGTGGGGGCGTGTGTTCGCCATTTTCTGGATTTTGATAAGTACCATCACTCTGGCACAATTTTTCCTTTACATTGCTGAGCTAAACACTGAAAGAAGACAAAGGTCATTGGTAAAGTGGGTTCTAAGTAGAAAGGTGACCGACATAGACCTCGAGGCAGCGGATATTGATGACGACGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTGAAAGAGATGGGGAAAATTACTGAAGATGACATTTCACCTGTGCTGAAGGAGTTCGAGGATCTTGATTTTGATCAATCAGGAACCTTGTCCATATCCGATATAACACTTGCCCAATTGTCT

Protein sequence

MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS

Homology

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BLAST of MS002682 vs. NCBI nr
Match: XP_022139396.1 (two-pore potassium channel 1 [Momordica charantia])

HSP 1 Score: 690.6 bits (1781), Expect = 6.9e-195
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV
Sbjct: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC
Sbjct: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           DTTKEYDTNKARNKCIVVFLLLLFFIISG TLLVTLEKLDLIDAFYCVCSTITTLGYGDK
Sbjct: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGTTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI
Sbjct: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 356

[Close]

BLAST of MS002682 vs. NCBI nr
Match: TYK07941.1 (two-pore potassium channel 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 570.9 bits (1470), Expect = 7.9e-159
Identity = 295/356 (82.87%), Postives = 318/356 (89.33%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M S++ARQPLL TSSNT  TR+IDIPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSEDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHAN-SPTEITRTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL +YLGIGTLCFYL R QIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVF+GMAL+GLILSNAADYLVEKQEI+LF A H +QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKC+VVFLLLL FIISG   LV +EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS+KVTDIDLE ADI
Sbjct: 241 SFSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

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BLAST of MS002682 vs. NCBI nr
Match: XP_008462994.1 (PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo])

HSP 1 Score: 570.1 bits (1468), Expect = 1.4e-158
Identity = 295/356 (82.87%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M SK+ARQPLL TSSNT  TR+IDIPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHAN-SPTEITRTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL +YLGIGTLCFYL R QIKGEK+NGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVF+GMAL+GLILSNAADYLVEKQEI+LF A H +QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKC+VVFLLLL FIISG   LV +EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS KVTDIDLE ADI
Sbjct: 241 SFSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

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BLAST of MS002682 vs. NCBI nr
Match: XP_004150789.1 (two-pore potassium channel 1 [Cucumis sativus] >KGN44008.1 hypothetical protein Csa_018211 [Cucumis sativus])

HSP 1 Score: 562.8 bits (1449), Expect = 2.2e-156
Identity = 294/356 (82.58%), Postives = 316/356 (88.76%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M S++ARQPLL TSSNT  TR+I+IPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHAN-SPTEITHTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL  YLGIGTLCFYL R+QI+GEKTN +VDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVFTGMAL+GLILSNAADYLVEKQEI+LF A H  QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKCIVVFLLLL FIISG   LVT+EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGRVFAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS+KVTDIDLE ADI
Sbjct: 241 SFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDIS VL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of MS002682 vs. NCBI nr
Match: XP_038896135.1 (two-pore potassium channel 1 [Benincasa hispida])

HSP 1 Score: 555.8 bits (1431), Expect = 2.6e-154
Identity = 291/358 (81.28%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGT--ATG 60
           M S+EARQPLL TSSNT   RLIDIPRS+RRLRR+KSAP+ D   P T   CTG   AT 
Sbjct: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYAD--SPHTEIACTGNVPATC 60

Query: 61  SVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTM 120
            VPRSG +F NLHPS RRVA+VL +YLGIGTLCFYL R QIKGEKTNGIVDAIYFTIVTM
Sbjct: 61  PVPRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTM 120

Query: 121 TTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPG 180
           TTVGYGDLVPNSP  KLLAC+FVFTGMALIGLILSNAADYLVEKQE +LF A H +QN  
Sbjct: 121 TTVGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQN-S 180

Query: 181 PCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYG 240
            CD ++E DTNKARNKCIVVFL+LL FIISG   LV+LEKLD IDAFYCVCSTITTLGYG
Sbjct: 181 HCDISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYG 240

Query: 241 DKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAA 300
           D+SFST WGR+FAIFWILISTITLAQFFLYIAELNTERRQ+SLVKW+LS+KVTDIDLE A
Sbjct: 241 DQSFSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVA 300

Query: 301 DIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           D+DDDGVVGAAEFVIYKLKEMGKITEDDISPVL+EFE+LD DQSGTLS SDITLAQ S
Sbjct: 301 DLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 355

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BLAST of MS002682 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 398.7 bits (1023), Expect = 7.1e-110
Identity = 214/366 (58.47%), Postives = 264/366 (72.13%), Query Frame = 0


Query: 1   MASKEARQPLLST------------SSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPET 60
           M+S  AR PLL T            +S TS +R       KRRLRRS+SAP GD    + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  TSTCTGTATGSVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIV 120
                       P    +F +L+P+LRRV + L +YL IGTLCFYL R+QI G KT+G+V
Sbjct: 61  VKI---DEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLF 180
           DA+YF IVTMTTVGYGDLVPNS  ++LLAC+FVF+GM L+G +LS AADYLVEKQE +L 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 NALHTYQNPGPCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCV 240
            A H  Q+ GP D  KE  TNK R KC    L+L+   I G   LV +EK+ +I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSR 300
           CST+TTLGYGDKSF++  GR+FA+FWIL S+I LAQFFLY+AELNTE +QR+LVKWVL+R
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEAADIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSIS 355
           ++T+ DLEAAD+D+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD+D+SGTL+ S
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 356

[Close]

BLAST of MS002682 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.6e-93
Identity = 184/356 (51.69%), Postives = 243/356 (68.26%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGD--YALPETTSTCTGTATG 60
           M     +Q LL+ + N  + +  +      R RR +S P  D     PE          G
Sbjct: 1   MDDNSIQQSLLADNPNVLQRKPSE---GVNRFRRCRSTPSTDPLQGPPE---------KG 60

Query: 61  SVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTM 120
           S  ++  +F  + PS R V ++L +YL +G L FY   ++I G++TN ++DA+YF +VTM
Sbjct: 61  SSVKAKELFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTM 120

Query: 121 TTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPG 180
           TTVGYGDLVPN+   KLLAC+FVF GMA++ L +S  ADYLVEKQE++ F ALHT    G
Sbjct: 121 TTVGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGG 180

Query: 181 PCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYG 240
                +  +TN+ + K     LLL+  IISG   L  +EKL L+D+FYCVC+TITTLGYG
Sbjct: 181 ETKMLRAIETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYG 240

Query: 241 DKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAA 300
           DKSFS++ GRVFA+FWI+ STI +AQFF+Y+AE+ TERRQ+ L  WVL+RK+T +DLEAA
Sbjct: 241 DKSFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAA 300

Query: 301 DIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQ 355
           D+DDD  VGAAEFV+YKLKE+GKI +++IS  L+EFE LD D SGTLS  D+TLAQ
Sbjct: 301 DLDDDRQVGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQ 344

[Close]

BLAST of MS002682 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.9e-87
Identity = 170/325 (52.31%), Postives = 225/325 (69.23%), Query Frame = 0


Query: 30  RRLRRSKSAPHGDYALPETTSTCTGTATGSVPRSGFVFGNLHPSLRRVAIVLTVYLGIGT 89
           RR RR ++AP  +   P  T     +A  + P++ F  G   PS R V ++L  YL +GT
Sbjct: 25  RRFRRCRTAPSSE---PPPTDKDNSSAADAPPKTLFTGGG-RPSFRLVGLLLVAYLLLGT 84

Query: 90  LCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGAKLLACSFVFTGMALIG 149
           + FYLA + + G +T   +DA+YF +VTMTTVGYGDLVP S  AKLLAC+FVF G+A++G
Sbjct: 85  IAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVG 144

Query: 150 LILSNAADYLVEKQEIMLFNALHTYQNPGPCDTTKEYDTNKARNKCIVVFLLLLFFIISG 209
             LS AADYLVEKQE +LF ALH++         +  + NK R K     LLL+  + SG
Sbjct: 145 TFLSKAADYLVEKQEALLFRALHSH------TMVRAMEMNKVRYKLYTAGLLLVAAVASG 204

Query: 210 NTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFSTRWGRVFAIFWILISTITLAQFFLYI 269
             +L  +E +  +DAFYCVC+T+TTLGYGD+SFS+  GR FA+ WI +ST+ +A FFLY 
Sbjct: 205 TVVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYA 264

Query: 270 AELNTERRQRSLVKWVLSRKVTDIDLEAADIDDDGVVGAAEFVIYKLKEMGKITEDDISP 329
           AEL TERRQR L +WVL R+ T++DLEAAD+D D  VGAA+FV+YKLKE+GKI+++DIS 
Sbjct: 265 AELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISE 324

Query: 330 VLKEFEDLDFDQSGTLSISDITLAQ 355
            L EF++LD D SGTLS +D+  AQ
Sbjct: 325 FLDEFDNLDADHSGTLSPADLAAAQ 339

[Close]

BLAST of MS002682 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.9e-48
Identity = 122/342 (35.67%), Postives = 185/342 (54.09%), Query Frame = 0


Query: 22  LIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSVPRSGFVFGNLHPSLRRVAIV- 81
           L D    +  L RS++AP    A+    +     A  S         N  P  RR AIV 
Sbjct: 106 LFDFIGGRTNLHRSRTAP----AMAPLNAAAIAAAAASGDSR-----NPPPPPRRPAIVL 165

Query: 82  -----LTVYLGIGTLCFYLA---RNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPG 141
                L  YL +G + FY A           T+ + DA+YF IVT+ T+GYGD+ P +P 
Sbjct: 166 HAFLFLLAYLAMG-VTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPA 225

Query: 142 AKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPCDTTKEYDTNK-- 201
           AKL + SFV  G   + ++LS    Y+++ QE +L  AL   ++         +D  K  
Sbjct: 226 AKLFSISFVLIGFGFVDILLSGMVSYVLDLQEHLLITALKNPRSVRKHRHNYIFDLKKGR 285

Query: 202 --ARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFSTRWGR 261
              R K  +   ++   +  G  +L  +E L  +DA Y    ++TT+GYGD +F T  GR
Sbjct: 286 MRVRMKVALALTVVAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGR 345

Query: 262 VFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDDGVVGA 321
           +FA  W+L+ST+ +A+ FLY+AE+  ++R R++  WVLSR +T  +  AADID++G V  
Sbjct: 346 LFASAWLLVSTLAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTK 405

Query: 322 AEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDI 351
           +EFV+YKLKEMGKI+E DI  +  +F+ +D    G +++SD+
Sbjct: 406 SEFVVYKLKEMGKISEKDIMMICDQFQRMDSGNCGKITLSDL 437

[Close]

BLAST of MS002682 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 106/287 (36.93%), Postives = 170/287 (59.23%), Query Frame = 0


Query: 74  LRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGA 133
           +R+   +L VYL +G   +   R+   G +T+ +VDA+YF IVTM T+GYGD+ P +P  
Sbjct: 117 IRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWT 176

Query: 134 KLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPCD----TTKEY--D 193
           K+ A  FV  G   + ++LS   +Y+++ QE M+   + T Q+         + K+Y  D
Sbjct: 177 KIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIID 236

Query: 194 TNKARNKCIVVFLLLLFFII----SGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFS 253
             K R +  +   L L  ++     G  +L  +E+L  +D+ Y    ++TT+GYGD++F 
Sbjct: 237 FEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFK 296

Query: 254 TRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDD 313
           T  GR+FA  W+L+ST+ +A+ FLY+AE   +RR R  VK  L+R++T  DL  AD    
Sbjct: 297 TLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQH 356

Query: 314 GVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDI 351
           G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 357 GFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of MS002682 vs. ExPASy TrEMBL
Match: A0A6J1CCI8 (two-pore potassium channel 1 OS=Momordica charantia OX=3673 GN=LOC111010336 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.3e-195
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV
Sbjct: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC
Sbjct: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           DTTKEYDTNKARNKCIVVFLLLLFFIISG TLLVTLEKLDLIDAFYCVCSTITTLGYGDK
Sbjct: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGTTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI
Sbjct: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 356

[Close]

BLAST of MS002682 vs. ExPASy TrEMBL
Match: A0A5D3C7V3 (Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00580 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.8e-159
Identity = 295/356 (82.87%), Postives = 318/356 (89.33%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M S++ARQPLL TSSNT  TR+IDIPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSEDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHAN-SPTEITRTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL +YLGIGTLCFYL R QIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVF+GMAL+GLILSNAADYLVEKQEI+LF A H +QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKC+VVFLLLL FIISG   LV +EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS+KVTDIDLE ADI
Sbjct: 241 SFSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of MS002682 vs. ExPASy TrEMBL
Match: A0A1S3CI53 (two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.6e-159
Identity = 295/356 (82.87%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M SK+ARQPLL TSSNT  TR+IDIPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHAN-SPTEITRTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL +YLGIGTLCFYL R QIKGEK+NGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVF+GMAL+GLILSNAADYLVEKQEI+LF A H +QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKC+VVFLLLL FIISG   LV +EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS KVTDIDLE ADI
Sbjct: 241 SFSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of MS002682 vs. ExPASy TrEMBL
Match: A0A0A0K3G0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.0e-156
Identity = 294/356 (82.58%), Postives = 316/356 (88.76%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M S++ARQPLL TSSNT  TR+I+IPRSKRRLRR+KSAPH + +  E T T    ATG V
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHAN-SPTEITHTSNVPATGPV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG +FGNLHPS RRVA+VL  YLGIGTLCFYL R+QI+GEKTN +VDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPNSP  KLLAC+FVFTGMAL+GLILSNAADYLVEKQEI+LF A H  QN G C
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQN-GHC 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D +KE DTNKARNKCIVVFLLLL FIISG   LVT+EKLD IDAFYCVCSTITTLGYGD+
Sbjct: 181 DISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQ 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFST+WGRVFAIFWILISTITLAQFFLYIAELNTERRQ+SLVKWVLS+KVTDIDLE ADI
Sbjct: 241 SFSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADI 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDIS VL EFE+LD DQSGTLSISDITLAQLS
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of MS002682 vs. ExPASy TrEMBL
Match: A0A6J1FCF3 (two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 288/356 (80.90%), Postives = 310/356 (87.08%), Query Frame = 0


Query: 1   MASKEARQPLLSTSSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPETTSTCTGTATGSV 60
           M S+E RQP+L TSSNT  TR IDIPRSKRRLRR+KSAPH      ETTS    TATG V
Sbjct: 1   MGSQEVRQPMLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTS----TATGLV 60

Query: 61  PRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG VFGNLHPS  RVA+VL +YLGIGTLCFYL  +QIKG KTNG+VDAIYFTIVTMTT
Sbjct: 61  PRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYLVGHQIKGGKTNGVVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPC 180
           VGYGDLVPN+P  KLLAC+FVFTGMA++GLIL+NAADYLVEKQEI LF A H +QN GP 
Sbjct: 121 VGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQN-GPF 180

Query: 181 DTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDK 240
           D TKE DTNKARNKCIVVFL LL FII G   LVTLE+LD IDAFYCVCSTITTLGYGDK
Sbjct: 181 DITKEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDK 240

Query: 241 SFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADI 300
           SFSTRWGR+FAIFWILIS+ITLAQFFLYIA LNTERR++SLVKWVLS+KVT +DLEAAD+
Sbjct: 241 SFSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADL 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDITLAQLS 357
           DDDGVVGAAEFVIYKLKEMGKITEDDISPVL EFE+LD DQSGTLSISDITLAQ S
Sbjct: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

[Close]

BLAST of MS002682 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 398.7 bits (1023), Expect = 5.0e-111
Identity = 214/366 (58.47%), Postives = 264/366 (72.13%), Query Frame = 0


Query: 1   MASKEARQPLLST------------SSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPET 60
           M+S  AR PLL T            +S TS +R       KRRLRRS+SAP GD    + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  TSTCTGTATGSVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIV 120
                       P    +F +L+P+LRRV + L +YL IGTLCFYL R+QI G KT+G+V
Sbjct: 61  VKI---DEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLF 180
           DA+YF IVTMTTVGYGDLVPNS  ++LLAC+FVF+GM L+G +LS AADYLVEKQE +L 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 NALHTYQNPGPCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCV 240
            A H  Q+ GP D  KE  TNK R KC    L+L+   I G   LV +EK+ +I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSR 300
           CST+TTLGYGDKSF++  GR+FA+FWIL S+I LAQFFLY+AELNTE +QR+LVKWVL+R
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEAADIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSIS 355
           ++T+ DLEAAD+D+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD+D+SGTL+ S
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 356

[Close]

BLAST of MS002682 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 398.7 bits (1023), Expect = 5.0e-111
Identity = 214/366 (58.47%), Postives = 264/366 (72.13%), Query Frame = 0


Query: 1   MASKEARQPLLST------------SSNTSRTRLIDIPRSKRRLRRSKSAPHGDYALPET 60
           M+S  AR PLL T            +S TS +R       KRRLRRS+SAP GD    + 
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSR-------KRRLRRSRSAPRGDCMYNDD 60

Query: 61  TSTCTGTATGSVPRSGFVFGNLHPSLRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIV 120
                       P    +F +L+P+LRRV + L +YL IGTLCFYL R+QI G KT+G+V
Sbjct: 61  VKI---DEPPPHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVV 120

Query: 121 DAIYFTIVTMTTVGYGDLVPNSPGAKLLACSFVFTGMALIGLILSNAADYLVEKQEIMLF 180
           DA+YF IVTMTTVGYGDLVPNS  ++LLAC+FVF+GM L+G +LS AADYLVEKQE +L 
Sbjct: 121 DALYFCIVTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLV 180

Query: 181 NALHTYQNPGPCDTTKEYDTNKARNKCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCV 240
            A H  Q+ GP D  KE  TNK R KC    L+L+   I G   LV +EK+ +I AFYCV
Sbjct: 181 RAFHLRQSFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCV 240

Query: 241 CSTITTLGYGDKSFSTRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSR 300
           CST+TTLGYGDKSF++  GR+FA+FWIL S+I LAQFFLY+AELNTE +QR+LVKWVL+R
Sbjct: 241 CSTVTTLGYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTR 300

Query: 301 KVTDIDLEAADIDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSIS 355
           ++T+ DLEAAD+D+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD+D+SGTL+ S
Sbjct: 301 RITNNDLEAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTS 356

[Close]

BLAST of MS002682 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 191.8 bits (486), Expect = 9.3e-49
Identity = 106/287 (36.93%), Postives = 170/287 (59.23%), Query Frame = 0


Query: 74  LRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGA 133
           +R+   +L VYL +G   +   R+   G +T+ +VDA+YF IVTM T+GYGD+ P +P  
Sbjct: 117 IRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWT 176

Query: 134 KLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPCD----TTKEY--D 193
           K+ A  FV  G   + ++LS   +Y+++ QE M+   + T Q+         + K+Y  D
Sbjct: 177 KIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIID 236

Query: 194 TNKARNKCIVVFLLLLFFII----SGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFS 253
             K R +  +   L L  ++     G  +L  +E+L  +D+ Y    ++TT+GYGD++F 
Sbjct: 237 FEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFK 296

Query: 254 TRWGRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDD 313
           T  GR+FA  W+L+ST+ +A+ FLY+AE   +RR R  VK  L+R++T  DL  AD    
Sbjct: 297 TLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQH 356

Query: 314 GVVGAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDI 351
           G +  +E+++ KLKEMGKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 357 GFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of MS002682 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 105/283 (37.10%), Postives = 168/283 (59.36%), Query Frame = 0


Query: 74  LRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGA 133
           +R+   +L VYL +G L ++L R+     +T+ +VD +YF IVTM T+GYGD+ PNS   
Sbjct: 150 VRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVT 209

Query: 134 KLLACSFVFTGMALIGLILSNAADYLVEKQEIMLFNALHTYQNPGPCDTTKEY--DTNKA 193
           KL +  FV  G   I ++LS    Y+++ QE  + ++      P   +  + Y  D  K 
Sbjct: 210 KLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEP---EKRRSYIIDVKKG 269

Query: 194 RN----KCIVVFLLLLFFIISGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFSTRWG 253
           R     K  +   +++  I  G  ++  +E++  +D+FY    ++TT+GYGD++F T  G
Sbjct: 270 RMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPG 329

Query: 254 RVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDDGVVG 313
           R+FA  W+L+ST+ +A+ FLY+AE   ++R R   K VL   ++     AADID++G V 
Sbjct: 330 RLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVS 389

Query: 314 AAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDI 351
            AE+VIYKLKEM KIT+ DI P+ K+F+ LD   +G +++ D+
Sbjct: 390 KAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

[Close]

BLAST of MS002682 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 183.0 bits (463), Expect = 4.3e-46
Identity = 105/284 (36.97%), Postives = 167/284 (58.80%), Query Frame = 0


Query: 74  LRRVAIVLTVYLGIGTLCFYLARNQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPGA 133
           + +   +L VYL +G L ++L R+    ++T+ +VDA+YF IVTM T+GYGD+ P+S   
Sbjct: 146 VNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVT 205

Query: 134 KLLACSFVFTGMALIGLILSNAADYLVEKQE-IMLFNALHTYQNPGPCDTTKEY--DTNK 193
           KL +  FV  G   + ++LS    Y+++ QE  ML  A +   N    D  + Y  D  K
Sbjct: 206 KLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKK 265

Query: 194 ARNKCIVVFLLLLFFII----SGNTLLVTLEKLDLIDAFYCVCSTITTLGYGDKSFSTRW 253
            R +  +   L L  ++     G  ++  +EK+  +D+FY    ++TT+GYGD++F+T  
Sbjct: 266 GRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLA 325

Query: 254 GRVFAIFWILISTITLAQFFLYIAELNTERRQRSLVKWVLSRKVTDIDLEAADIDDDGVV 313
           GR+ A  W+L+ST+ +A+  L++AE   ++R R   K VL   ++      ADID +G V
Sbjct: 326 GRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCV 385

Query: 314 GAAEFVIYKLKEMGKITEDDISPVLKEFEDLDFDQSGTLSISDI 351
             AEFVIYKLK+M KITE DI+P+  +F+ LD   SG +++ D+
Sbjct: 386 SKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDL 429

The following BLAST results are available for this feature:

BLAST of MS002682 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139396.1	6.9e-195	99.72	two-pore potassium channel 1 [Momordica charantia]	[more]
TYK07941.1	7.9e-159	82.87	two-pore potassium channel 1 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008462994.1	1.4e-158	82.87	PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo]	[more]
XP_004150789.1	2.2e-156	82.58	two-pore potassium channel 1 [Cucumis sativus] >KGN44008.1 hypothetical protein ...	[more]
XP_038896135.1	2.6e-154	81.28	two-pore potassium channel 1 [Benincasa hispida]	[more]

BLAST of MS002682 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBL1	7.1e-110	58.47	Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2	[more]
Q850M0	1.6e-93	51.69	Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE...	[more]
Q8LIN5	4.9e-87	52.31	Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE...	[more]
Q69TN4	5.9e-48	35.67	Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE...	[more]
Q9S6Z8	1.3e-47	36.93	Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1	[more]

BLAST of MS002682 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCI8	3.3e-195	99.72	two-pore potassium channel 1 OS=Momordica charantia OX=3673 GN=LOC111010336 PE=3...	[more]
A0A5D3C7V3	3.8e-159	82.87	Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CI53	6.6e-159	82.87	two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 ...	[more]
A0A0A0K3G0	1.0e-156	82.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1	[more]
A0A6J1FCF3	2.0e-152	80.90	two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 ...	[more]

BLAST of MS002682 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55630.1	5.0e-111	58.47	Outward rectifying potassium channel protein	[more]
AT5G55630.2	5.0e-111	58.47	Outward rectifying potassium channel protein	[more]
AT4G01840.1	9.3e-49	36.93	Ca2+ activated outward rectifying K+ channel 5	[more]
AT4G18160.1	1.6e-48	37.10	Ca2+ activated outward rectifying K+ channel 6	[more]
AT5G46370.1	4.3e-46	36.97	Ca2+ activated outward rectifying K+ channel 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003280	Two pore domain potassium channel	PRINTS	PR01333	2POREKCHANEL	coord: 233..242 score: 45.91 coord: 116..144 score: 29.7
IPR003280	Two pore domain potassium channel	PANTHER	PTHR11003	POTASSIUM CHANNEL, SUBFAMILY K	coord: 21..350
None	No IPR available	GENE3D	1.10.287.70		coord: 71..165 e-value: 5.5E-23 score: 82.6
None	No IPR available	GENE3D	1.10.287.70		coord: 181..292 e-value: 1.8E-18 score: 68.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..56
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR11003:SF270	TWO PORE POTASSIUM CHANNEL A	coord: 21..350
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 189..294
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 71..166
IPR013099	Potassium channel domain	PFAM	PF07885	Ion_trans_2	coord: 202..272 e-value: 2.0E-13 score: 50.1 coord: 79..160 e-value: 1.2E-17 score: 63.6
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 299..311
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 285..351

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002682.1	MS002682.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071805	potassium ion transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0005267	potassium channel activity