MS002616 (gene) Bitter gourd (TR) v1

Overview


Name	MS002616
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold111: 158952 .. 162407 (-)
RNA-Seq Expression	MS002616
Synteny	MS002616

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAATCTCAACAATCTCATCACCATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCATCCCGCTCCTCGCTTCCTCTCCGACCTTGACTTCGCCTTCCTCCGCGATCACGTCACGTGTCGTCGTCACCACCACCACCATCACCATCACCGTCGTCATGAAAGCCCGGCCGATGAAGAAGACCAGGCGGCTTCTAGTTCCATATGTGACATGTTCCCGGCTAGCCTTCTCCCGACAGAAACCAACACCACCTCCGTCTTCTGCGTCGACCGCAACGGGTGCTGCAACTTCACGACCGTTCAGGCGGCGGTCGACGCGGTGCCGAGCCTGAGCCTGAAACGAAACTTGATTTGGATCAACAGCGGAATCTACTAGTAATTATATAATGCTAAATATGTTGATCGAATCCACGGACTCTTTGTTGAAGATATATTCTTGATTTTGGTTATGGATTATTGTATGTTTGTTTCGCAGTGAGAAAGTGATGGTTCCCAGATCAAAACAAAACGTGACGTTTGAAGGACAGGGCTATACGACGACGGCCATTGTTTGGAACGACACGGCCAACTCTTCTCATGGCACCTTTTACAGTGCCTCTGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGGTATATATATTAATTATCATTTTTAATATTTTAAATCACATTTTTAATATTATCATTTTTTTAGTTCAACCAAAATAGGGATTATTTAGGTTTAGATTAAAAAAGATAATAATGCTTATTGGATCTTTATAACTCATTTTTAAACGTAGTCACAAATTATTTAGTCGAGTTTGCCGTAATTATTATAATAGGATGGTAGAGATTTGAAGCTAATTTGCCAGAGTATTTTTATAGCTATATTTATTTACAGTTAAATTATAGTAAATGATAAATATGTTTGTTAGAACGAGTTTAATTGAACGATAATTAACATATATCTCTAACCGAGCTTGTGAATTCGATCCACTGAATCCAACTAAAAAATTGACAAACATGTTTAATAACTTAAAACTATGATAACTATTGATTTTTTTTCTTTTTTTTTTCGAGGAATGATGAAATTAGCTAGGTTAAATTTTAACGATGAAATATATGTAATTAAACCTCAATTTTAGGTTAATTATATTTACTTTTGTCGACCAGTTTTTTCTTTTTCTTTCTTTTTTTTTCCAGAAAATTTTGTCTGCCAGTTTGATAACGGTTTGATAATTATTTTTTTTTTTTTTAATATATTATATTTGTTTACTAATAATTTTTTTTTTGATNNNNNNNACCATTTTTTTAATTTATAATCAAGGTGTGTAAAAACATTTTTTAAATTTTAAGACCTGTTTTTAGTTTCTGAATTTTGTTAAAACCTAAAAGTCAATTTTATAAAAAAGAAAAAACTTAAAAGTCATTTTGTATGGTGAAAAATGTAGACAAAGATCTAATTATTAGGAAATAAGGTTAATTAAAAAAAAAAAACTTCACACTTAAGATTCAAACAAAATAAATTTGTTGTTGCCTTAAAAAAAAAATTTGATAATTCATGATTTGAACCTACAAAATTAGCCAACCTCAATCTCATGATTGATGTTCAAATCTCCTACGCTCGCTCCCATCGATGAATTAAAAAAGAAAAAATATTGAACCCTAAGTCCTGTTTTAATTTCGTTAGGATTAATCAATAAATGTAAATCCAAAAAAGAAATTCCCCAACAAAAACCAAAATCCAACCAAATTAATTAACAAAATTTGTGGCAGAACGTGGCGCCAATTCCGGGGCCGGGCGATGTGGGAGCGCAGGCGGTGGCGATGAGAATCGGCGGAGACGCGGCGGCGTTTTGGAGCTGCGGATTCTTCGGAGCCCAAGACACGCTCCACGACGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGGTAATCGCAATCGGAGCCCTAATTAATTAATTAATTATTTTTAATTTTGAAGCGCTAAATCAAATATAAAATAAAATGAAATCGCAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCGCAAGGGTCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCCGACGAGAACTCTGGTTTTGCTTTCGTTAACTGCTCCGTCGCCGGGACCGGCAGGGTGTGGCTCGGCCGCGCGTGGCGTCCCTTCTCTCGCGTCGTCTTCGTGAATACTGTCATGACCGATATTATTGCACCCGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGTACGTTCCCTTCCCTTCCCTTCCCATAATTTTAAACTTAAAAAAGGAAAACAAACAAATAATCTAGTCACTTATATTATAAATAAATAAATTTAAATTTAAAATTATTTATGATTTTTTTTTCTCCATTGAGCTTAGCAAGAGAAGTGTAGCTTTTAAATCTTAATATTGCCATTATTTCTTACTAATTTTAACATGAAAATCTTTCGACAAGAGTAATTTCAATATGGAAATCAAAACCTAAAAAAGATAAGATCACAATATTAACTATGAATATACAATAATTTGTTAATGTAGGTTGAGGATCCCACATCGATTAGGTAAGAGATCTAACAAATATTTATAATATAAGTGTACTACTCATTTCATTGTCAATTGAATTGGAATGAATATTAATAATTTTAATAGGGTATCATAACCCAAAGGCTCAAACGGACATCCGGTCCATAAATCAGATTATCAATGAACTAAAAGAGCCACGATCTTGAGAAGGTCATTGTCATTGTCAATTGATTTTGATATAAAATCCTATAAATATTAATAATTTTAGTAATGCACATGTGCTAACTTAGGTTAAGAAGTCAAAGTTCATATCTAGTATTACTTCATGTCATATATATTTAAACTTTTAAAAAGTTTACTTTGATTCTTTTAACTTCTAAAAACTACTATTTTGATTTAATTTTTTTCAATCATAAATTTAGTATGACACTCAACTCCTTAAGTATTAATTTTTTGAAATGTAATCATAATTTGTATTAAAAAAGTTTACATTTGGTATAAAATTTGAGTTAAAATTTTGTAAATAGAATAAAATTGTTTCATTTTTTTAAGTTGCCAATATGAGACCAATAATTGAAATAAACAAAAAATTGTAACATAAAAATATAGAGTCGAAAATATTATTTAAAACTTTAAAGTATTAATTGAGACAACTGATTTGATTTGATGTGTTGATATTTCAGGACGATCTTCTATGGAGAGTACAACTGCAGTGGCGCAGGCGCCAATACCAGCGCCAGGGCGCCGTTCGTTCAGAGGCTCAACGACACACAAGTTTCTCCTTTTCTCAACTTGTCCTTTATTGATGCAGACCAATGGCTACATCCCTAT

mRNA sequence

ATGAATAATCTCAACAATCTCATCACCATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCATCCCGCTCCTCGCTTCCTCTCCGACCTTGACTTCGCCTTCCTCCGCGATCACGTCACGTGTCGTCGTCACCACCACCACCATCACCATCACCGTCGTCATGAAAGCCCGGCCGATGAAGAAGACCAGGCGGCTTCTAGTTCCATATGTGACATGTTCCCGGCTAGCCTTCTCCCGACAGAAACCAACACCACCTCCGTCTTCTGCGTCGACCGCAACGGGTGCTGCAACTTCACGACCGTTCAGGCGGCGGTCGACGCGGTGCCGAGCCTGAGCCTGAAACGAAACTTGATTTGGATCAACAGCGGAATCTACTATGAGAAAGTGATGGTTCCCAGATCAAAACAAAACGTGACGTTTGAAGGACAGGGCTATACGACGACGGCCATTGTTTGGAACGACACGGCCAACTCTTCTCATGGCACCTTTTACAGTGCCTCTGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAACGTGGCGCCAATTCCGGGGCCGGGCGATGTGGGAGCGCAGGCGGTGGCGATGAGAATCGGCGGAGACGCGGCGGCGTTTTGGAGCTGCGGATTCTTCGGAGCCCAAGACACGCTCCACGACGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCGCAAGGGTCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCCGACGAGAACTCTGGTTTTGCTTTCGTTAACTGCTCCGTCGCCGGGACCGGCAGGGTGTGGCTCGGCCGCGCGTGGCGTCCCTTCTCTCGCGTCGTCTTCGTGAATACTGTCATGACCGATATTATTGCACCCGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGACGATCTTCTATGGAGAGTACAACTGCAGTGGCGCAGGCGCCAATACCAGCGCCAGGGCGCCGTTCGTTCAGAGGCTCAACGACACACAAGTTTCTCCTTTTCTCAACTTGTCCTTTATTGATGCAGACCAATGGCTACATCCCTAT

Coding sequence (CDS)

ATGAATAATCTCAACAATCTCATCACCATTGCTCTCTCTTCTCTCATCCCTCTCCTTGCTTTCCTCCTCATCAATCATCCCGCTCCTCGCTTCCTCTCCGACCTTGACTTCGCCTTCCTCCGCGATCACGTCACGTGTCGTCGTCACCACCACCACCATCACCATCACCGTCGTCATGAAAGCCCGGCCGATGAAGAAGACCAGGCGGCTTCTAGTTCCATATGTGACATGTTCCCGGCTAGCCTTCTCCCGACAGAAACCAACACCACCTCCGTCTTCTGCGTCGACCGCAACGGGTGCTGCAACTTCACGACCGTTCAGGCGGCGGTCGACGCGGTGCCGAGCCTGAGCCTGAAACGAAACTTGATTTGGATCAACAGCGGAATCTACTATGAGAAAGTGATGGTTCCCAGATCAAAACAAAACGTGACGTTTGAAGGACAGGGCTATACGACGACGGCCATTGTTTGGAACGACACGGCCAACTCTTCTCATGGCACCTTTTACAGTGCCTCTGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAACGTGGCGCCAATTCCGGGGCCGGGCGATGTGGGAGCGCAGGCGGTGGCGATGAGAATCGGCGGAGACGCGGCGGCGTTTTGGAGCTGCGGATTCTTCGGAGCCCAAGACACGCTCCACGACGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAACGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTGCCGCAAGGGTCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCCGACGAGAACTCTGGTTTTGCTTTCGTTAACTGCTCCGTCGCCGGGACCGGCAGGGTGTGGCTCGGCCGCGCGTGGCGTCCCTTCTCTCGCGTCGTCTTCGTGAATACTGTCATGACCGATATTATTGCACCCGAGGGGTGGAATGACTTTAATGACCCTGCCAGAGACCAGACGATCTTCTATGGAGAGTACAACTGCAGTGGCGCAGGCGCCAATACCAGCGCCAGGGCGCCGTTCGTTCAGAGGCTCAACGACACACAAGTTTCTCCTTTTCTCAACTTGTCCTTTATTGATGCAGACCAATGGCTACATCCCTAT

Protein sequence

MNNLNNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY

Homology

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BLAST of MS002616 vs. NCBI nr
Match: XP_022136420.1 (probable pectinesterase 8 [Momordica charantia])

HSP 1 Score: 802.4 bits (2071), Expect = 1.8e-228
Identity = 390/393 (99.24%), Postives = 392/393 (99.75%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60
           MNNLNNL+TIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE
Sbjct: 1   MNNLNNLMTIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60

Query: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKR 120
           SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVP+LSLKR
Sbjct: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLSLKR 120

Query: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180
           NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF
Sbjct: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180

Query: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240
           IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY
Sbjct: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240

Query: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300
           IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV
Sbjct: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300

Query: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360
           AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT
Sbjct: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360

Query: 361 SARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
            ARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY
Sbjct: 361 GARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

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BLAST of MS002616 vs. NCBI nr
Match: XP_038903629.1 (probable pectinesterase 8 [Benincasa hispida])

HSP 1 Score: 688.3 bits (1775), Expect = 3.8e-194
Identity = 339/400 (84.75%), Postives = 359/400 (89.75%), Query Frame = 0


Query: 1   MNNL---NNLIT-IALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHH 60
           MNN+   N+++T +ALSSL+PLLAFL +  P P FLS+L     RD + C  HHHHHHHH
Sbjct: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNL--VIFRDSIGCHHHHHHHHHH 60

Query: 61  ---RRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAV 120
              R H++ ADEEDQAA SSICD FP +LLP ET  TSVFCVDRNGCCNFTTVQAAVDAV
Sbjct: 61  HHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAV 120

Query: 121 PSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASV 180
           P LS KRN+IWIN GIYYEKV+VP+SK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SV
Sbjct: 121 PILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSV 180

Query: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGR 240
           QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGR
Sbjct: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGR 240

Query: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300
           HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF
Sbjct: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300

Query: 301 AFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360
            FVNCS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC
Sbjct: 301 VFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360

Query: 361 SGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
           +GAGAN S+RAPFVQRLNDTQ SPFLNLSFIDADQWL PY
Sbjct: 361 NGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 398

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BLAST of MS002616 vs. NCBI nr
Match: XP_008451885.1 (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 667.2 bits (1720), Expect = 9.0e-188
Identity = 327/398 (82.16%), Postives = 353/398 (88.69%), Query Frame = 0


Query: 1   MNNL-----NNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHH 60
           MNN+     N LI + LSSL+PLLAFL + +P P +LS+L      D V C   HHHHH 
Sbjct: 1   MNNIMTKYNNFLINLTLSSLLPLLAFLFMINPTPSYLSNL--VVFGDPVDCPHPHHHHH- 60

Query: 61  HRRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPS 120
              HE P DE+DQ+  SSICD FP +L+P ET  TSVFCVD+NGCCNFTTVQAAVDAVP+
Sbjct: 61  ---HEDP-DEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPN 120

Query: 121 LSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQV 180
           LS+KRN+IWIN+GIYYEKVMVP+SK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQV
Sbjct: 121 LSIKRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180

Query: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHY 240
           FSSNFIAKNISFMNVAPIPGPGDVGAQAVA+R+GGD AAFWSCGFFGAQDTLHDDRGRHY
Sbjct: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHY 240

Query: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAF 300
           FKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+F
Sbjct: 241 FKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSF 300

Query: 301 VNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG 360
           VNCS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDP RDQTIFYGEYNCSG
Sbjct: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSG 360

Query: 361 AGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
           AGAN S+RAP+VQRLNDTQVS FLNLSFIDADQWL PY
Sbjct: 361 AGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

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BLAST of MS002616 vs. NCBI nr
Match: XP_004147773.1 (probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa_014669 [Cucumis sativus])

HSP 1 Score: 666.8 bits (1719), Expect = 1.2e-187
Identity = 324/393 (82.44%), Postives = 352/393 (89.57%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60
           MN  + LI + LSSL+PLLAFL + +P P FLS+L      D V C  HHH HHH   HE
Sbjct: 5   MNYNHFLINLTLSSLLPLLAFLFMINPTPSFLSNL--VVFGDPVDCPHHHHPHHH---HE 64

Query: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKR 120
           +P DE+DQ+  SSICD FP +L+P ET  TS+FCVD+N CCNFTT+QAAVDAVP+LS+KR
Sbjct: 65  NP-DEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKR 124

Query: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180
           N+IWIN+GIYYEKVMVP++K NVTF+GQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF
Sbjct: 125 NIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 184

Query: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240
           IAKN+SFMNVAPIP PGDVGAQ VA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFKDCY
Sbjct: 185 IAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCY 244

Query: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300
           IQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCS+
Sbjct: 245 IQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSI 304

Query: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360
            GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 
Sbjct: 305 GGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANM 364

Query: 361 SARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
           S+RAP+VQRLNDTQVS FLNLSFIDADQWL PY
Sbjct: 365 SSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

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BLAST of MS002616 vs. NCBI nr
Match: KAG7015100.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 651.0 bits (1678), Expect = 6.7e-183
Identity = 325/394 (82.49%), Postives = 347/394 (88.07%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPL-LAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRH 60
           M   N LI IALSSL+PL LA LL+  P P FLS LD     D ++C     HHHHH+  
Sbjct: 1   MTRNNVLINIALSSLVPLFLALLLVVIPTPPFLSVLD-----DSLSC-----HHHHHK-- 60

Query: 61  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLK 120
            S ADEED+A+  SICD FP +LLP ET  TSV CVD+NGCCNFTTVQAAVDAV ++S K
Sbjct: 61  -SSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSK 120

Query: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180
           R+LIWIN+GIY+EKVMVPRSK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQVFSSN
Sbjct: 121 RSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSN 180

Query: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240
           FIAKNISFMNVAPIPGPG +GAQAVA+RIGGD AAFW+CGFFGAQDTLHDDRGRHYFKDC
Sbjct: 181 FIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDC 240

Query: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           YIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS
Sbjct: 241 YIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300

Query: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGAN
Sbjct: 301 IGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGAN 360

Query: 361 TSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
            SARAP+VQRLNDTQ SPFLNLSFIDADQWL PY
Sbjct: 361 MSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 381

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BLAST of MS002616 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 526.9 bits (1356), Expect = 1.9e-148
Identity = 250/389 (64.27%), Postives = 309/389 (79.43%), Query Frame = 0


Query: 7   LITIALSSLIPLLAF-----LLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHES 66
           +I++++S  I ++A      L   HP    +  + ++F +       HHHHHHHH     
Sbjct: 3   IISLSISIGIAIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHHHHHHHRHK 62

Query: 67  PADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKRN 126
           P+D + +    SICD FP ++ P +T+TTS  CVD+NGCCNFTTVQ+AVDAV + S +RN
Sbjct: 63  PSDTKRKV---SICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRN 122

Query: 127 LIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFI 186
           +IWINSG+YYEKV++P++K N+T +GQG+  TAI WNDTA S++GTFY A+VQVF S F+
Sbjct: 123 VIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFV 182

Query: 187 AKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYI 246
           AKNISFMNVAPIP PGDVGAQAVA+RI GD +AF  CGFFGAQDTLHDDRGRHYFKDCYI
Sbjct: 183 AKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYI 242

Query: 247 QGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVA 306
           QGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+FVNC++ 
Sbjct: 243 QGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIG 302

Query: 307 GTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTS 366
           GTG VWLGRAWRP+SRVVFV+T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+ S
Sbjct: 303 GTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMS 362

Query: 367 ARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
            RAP+VQ+LN+TQV+  +N SFID DQWL
Sbjct: 363 KRAPYVQKLNETQVALLINTSFIDGDQWL 388

[Close]

BLAST of MS002616 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.2e-95
Identity = 186/388 (47.94%), Postives = 250/388 (64.43%), Query Frame = 0


Query: 7   LITIALSSLIPLLAFLLINHPAPRFLSDL--DFAFLRDHVTC--RRHHHHHHHHRRHESP 66
           ++T  ++ L  +LA   I  P+ R LS +      L  + T    +HHHHHHHH  H   
Sbjct: 6   VLTTLIALLSIILALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHY 65

Query: 67  ADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKRNL 126
              E        C+ +  S L  +  T+ V  VD +GC NF+ VQ+A+D VP LS  + L
Sbjct: 66  HHHE----PIKCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTL 125

Query: 127 IWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIA 186
           I +NSG Y EKV V  +K N+  +G+GY  T+I WNDTA S+  T  S S  VF++NF A
Sbjct: 126 IIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTA 185

Query: 187 KNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQ 246
            NISF N AP P PG+  AQAVA+RI GD AAF+ CGF+GAQDTL DD+GRH+FK+C+IQ
Sbjct: 186 YNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQ 245

Query: 247 GSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVAG 306
           GSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC + G
Sbjct: 246 GSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDG 305

Query: 307 TGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTSA 366
           +G + LGRAW  ++ VVF NT M+ II PEGWN++ D  +++T+ +GE+ C G GA+   
Sbjct: 306 SGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKE 365

Query: 367 RAPFVQRLNDTQVSPFLNLSFIDADQWL 391
           R  F ++L D++ S F+++SFID D+WL
Sbjct: 366 RVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of MS002616 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.2e-87
Identity = 162/331 (48.94%), Postives = 214/331 (64.65%), Query Frame = 0


Query: 61  SPADEEDQAA-SSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLK 120
           SP +  DQ     S CD    S  PT+   T V  V  NGC  F  VQ A+DA    S  
Sbjct: 12  SPIESVDQRIHHPSKCDHL--SKFPTK-GFTMVLKVSLNGCGRFKRVQDAIDASIGSSQS 71

Query: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180
           + LI I+ GIY E+ +V  +K N+  +G GY+ T+I WN+T  SS+GTF S SV VF   
Sbjct: 72  KTLILIDFGIYRERFIVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEK 131

Query: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240
           F A NISF N AP P PG V AQAVA+++ GD AAF+ CGF+G QDTL D  GRH+FK C
Sbjct: 132 FTAYNISFKNTAPAPNPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGC 191

Query: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           +I+GSIDFIFGNGRS YE C L S+A       +   G +TA+G+ +  + +GF FVNC 
Sbjct: 192 FIEGSIDFIFGNGRSLYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFVNCK 251

Query: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + G+ RVWLGRAWRP++RV+F  T M+ +++ +GWND  DP   +T++YGE+ C G GAN
Sbjct: 252 ITGSARVWLGRAWRPYARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGAN 311

Query: 361 TSARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
            S R  + + L+D + +PF N+SFID ++WL
Sbjct: 312 HSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

[Close]

BLAST of MS002616 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.0e-81
Identity = 145/295 (49.15%), Postives = 199/295 (67.46%), Query Frame = 0


Query: 102 NFTTVQAAVDAVPSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTA 161
           +FT +Q A+D++P ++  R +I +++G+Y EKV +P  K  +T EG+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 162 NSSH------GTFYSASVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAF 221
            +        GT+ SAS  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 222 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFV 281
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 282 NGAVTAHGRASADENSGFAFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWN 341
            GAVTA GR+S  E++GF+FV C V GTG ++LGRAW PFSRVVF  T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 342 DFNDPARDQTIFYGEYNCSGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +  PFL+L+FID  +W+
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

[Close]

BLAST of MS002616 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 6.1e-70
Identity = 149/347 (42.94%), Postives = 204/347 (58.79%), Query Frame = 0


Query: 53  HHHHRRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDA 112
           HHHHR+   P+  +                         V  V  NG   F +VQ AVD+
Sbjct: 46  HHHHRKWVGPSGHK-------------------------VITVSLNGHAQFRSVQDAVDS 105

Query: 113 VPSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTAN--SSHG---- 172
           +P  + K   I I  G Y EKV+VP +K  +TF+G G   TAI W+D A+   ++G    
Sbjct: 106 IPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLR 165

Query: 173 TFYSASVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDT 232
           T+ +ASV V+++ F A+NISF N AP P PG  G QAVA RI GD A F  CGF+GAQDT
Sbjct: 166 TYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDT 225

Query: 233 LHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRAS 292
           L DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A      S+F  G++ AHGR  
Sbjct: 226 LCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSIAAHGRTC 285

Query: 293 ADENSGFAFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPA-RDQT 352
            +E +GFAFV C V GTG +++GRA   +SR+V+  T    ++A  GW+D++  + + +T
Sbjct: 286 PEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKT 345

Query: 353 IFYGEYNCSGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHP 393
            F+G YNC G GA  +    + + L+     PF+  SF++   W+ P
Sbjct: 346 AFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

[Close]

BLAST of MS002616 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 8.7e-229
Identity = 390/393 (99.24%), Postives = 392/393 (99.75%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60
           MNNLNNL+TIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE
Sbjct: 1   MNNLNNLMTIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60

Query: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKR 120
           SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVP+LSLKR
Sbjct: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLSLKR 120

Query: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180
           NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF
Sbjct: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180

Query: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240
           IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY
Sbjct: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240

Query: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300
           IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV
Sbjct: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300

Query: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360
           AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT
Sbjct: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360

Query: 361 SARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
            ARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY
Sbjct: 361 GARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

[Close]

BLAST of MS002616 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 4.3e-188
Identity = 327/398 (82.16%), Postives = 353/398 (88.69%), Query Frame = 0


Query: 1   MNNL-----NNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHH 60
           MNN+     N LI + LSSL+PLLAFL + +P P +LS+L      D V C   HHHHH 
Sbjct: 1   MNNIMTKYNNFLINLTLSSLLPLLAFLFMINPTPSYLSNL--VVFGDPVDCPHPHHHHH- 60

Query: 61  HRRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPS 120
              HE P DE+DQ+  SSICD FP +L+P ET  TSVFCVD+NGCCNFTTVQAAVDAVP+
Sbjct: 61  ---HEDP-DEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPN 120

Query: 121 LSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQV 180
           LS+KRN+IWIN+GIYYEKVMVP+SK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQV
Sbjct: 121 LSIKRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180

Query: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHY 240
           FSSNFIAKNISFMNVAPIPGPGDVGAQAVA+R+GGD AAFWSCGFFGAQDTLHDDRGRHY
Sbjct: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHY 240

Query: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAF 300
           FKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+F
Sbjct: 241 FKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSF 300

Query: 301 VNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG 360
           VNCS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDP RDQTIFYGEYNCSG
Sbjct: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSG 360

Query: 361 AGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
           AGAN S+RAP+VQRLNDTQVS FLNLSFIDADQWL PY
Sbjct: 361 AGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of MS002616 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.7e-188
Identity = 324/393 (82.44%), Postives = 352/393 (89.57%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHE 60
           MN  + LI + LSSL+PLLAFL + +P P FLS+L      D V C  HHH HHH   HE
Sbjct: 5   MNYNHFLINLTLSSLLPLLAFLFMINPTPSFLSNL--VVFGDPVDCPHHHHPHHH---HE 64

Query: 61  SPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKR 120
           +P DE+DQ+  SSICD FP +L+P ET  TS+FCVD+N CCNFTT+QAAVDAVP+LS+KR
Sbjct: 65  NP-DEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKR 124

Query: 121 NLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 180
           N+IWIN+GIYYEKVMVP++K NVTF+GQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF
Sbjct: 125 NIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNF 184

Query: 181 IAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCY 240
           IAKN+SFMNVAPIP PGDVGAQ VA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFKDCY
Sbjct: 185 IAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCY 244

Query: 241 IQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSV 300
           IQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCS+
Sbjct: 245 IQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSI 304

Query: 301 AGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANT 360
            GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 
Sbjct: 305 GGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANM 364

Query: 361 SARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
           S+RAP+VQRLNDTQVS FLNLSFIDADQWL PY
Sbjct: 365 SSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of MS002616 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.1e-182
Identity = 323/394 (81.98%), Postives = 346/394 (87.82%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPL-LAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRH 60
           M + N LI IALSSL+PL LA LL+  P P F S  D     D ++C     HHHHH+  
Sbjct: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPD-----DSLSC-----HHHHHK-- 60

Query: 61  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLK 120
              ADEED+A+  SICD FP +LLP ET  TSV CVD+NGCCNFTTVQAAVDAV ++S K
Sbjct: 61  -PSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSK 120

Query: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180
           R+LIWIN+GIY+EKVMVPRSK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQVFSSN
Sbjct: 121 RSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSN 180

Query: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240
           FIAKNISFMNVAPIPGPG +GAQAVA+RIGGD AAFW+CGFFGAQDTLHDDRGRHYFKDC
Sbjct: 181 FIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDC 240

Query: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           YIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS
Sbjct: 241 YIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300

Query: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGAN
Sbjct: 301 IGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGAN 360

Query: 361 TSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
            SARAP+VQRLNDTQVSPFLNLSFIDADQWL PY
Sbjct: 361 MSARAPYVQRLNDTQVSPFLNLSFIDADQWLQPY 381

[Close]

BLAST of MS002616 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.0e-181
Identity = 323/394 (81.98%), Postives = 342/394 (86.80%), Query Frame = 0


Query: 1   MNNLNNLITIALSSLIPL-LAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRH 60
           M   N LI IALSSL+PL LA LL+  P P FLS LD     D + C    HHHHHH+  
Sbjct: 1   MTRNNVLINIALSSLVPLFLALLLVVIPTPPFLSVLD-----DSLNC----HHHHHHK-- 60

Query: 61  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLK 120
              ADEEDQ +  SICD FP +LLP ET  TSV CVD NGCCNFTTVQAAVDAV ++S K
Sbjct: 61  -PSADEEDQTSIGSICDTFPTALLPLETVDTSVLCVDLNGCCNFTTVQAAVDAVLNMSSK 120

Query: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180
           R+LIWIN+GIY+EKVMVPRSK NVTF+GQGY TTAIVWNDTANSSHGTFYS SVQVFSSN
Sbjct: 121 RSLIWINTGIYFEKVMVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFSSN 180

Query: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240
           FIAKNISFMNVAPIPGPG +GAQAVA+RIGGD AAFW+CGFFGAQDTLHDDRGRHYFKDC
Sbjct: 181 FIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDC 240

Query: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           YIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS
Sbjct: 241 YIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300

Query: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSG GAN
Sbjct: 301 IGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTGAN 360

Query: 361 TSARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 394
            SARAP+VQRLNDTQ SPFLNLSFIDADQWL PY
Sbjct: 361 MSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 382

[Close]

BLAST of MS002616 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.4e-149
Identity = 250/389 (64.27%), Postives = 309/389 (79.43%), Query Frame = 0


Query: 7   LITIALSSLIPLLAF-----LLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRHES 66
           +I++++S  I ++A      L   HP    +  + ++F +       HHHHHHHH     
Sbjct: 3   IISLSISIGIAIIAVLASKTLFKTHPEAFGIKAISYSFKKSLCDHHHHHHHHHHHHHRHK 62

Query: 67  PADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKRN 126
           P+D + +    SICD FP ++ P +T+TTS  CVD+NGCCNFTTVQ+AVDAV + S +RN
Sbjct: 63  PSDTKRKV---SICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVGNFSQRRN 122

Query: 127 LIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFI 186
           +IWINSG+YYEKV++P++K N+T +GQG+  TAI WNDTA S++GTFY A+VQVF S F+
Sbjct: 123 VIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFV 182

Query: 187 AKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYI 246
           AKNISFMNVAPIP PGDVGAQAVA+RI GD +AF  CGFFGAQDTLHDDRGRHYFKDCYI
Sbjct: 183 AKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYI 242

Query: 247 QGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVA 306
           QGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+FVNC++ 
Sbjct: 243 QGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIG 302

Query: 307 GTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTS 366
           GTG VWLGRAWRP+SRVVFV+T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+ S
Sbjct: 303 GTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMS 362

Query: 367 ARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
            RAP+VQ+LN+TQV+  +N SFID DQWL
Sbjct: 363 KRAPYVQKLNETQVALLINTSFIDGDQWL 388

[Close]

BLAST of MS002616 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 3.0e-96
Identity = 186/388 (47.94%), Postives = 250/388 (64.43%), Query Frame = 0


Query: 7   LITIALSSLIPLLAFLLINHPAPRFLSDL--DFAFLRDHVTC--RRHHHHHHHHRRHESP 66
           ++T  ++ L  +LA   I  P+ R LS +      L  + T    +HHHHHHHH  H   
Sbjct: 6   VLTTLIALLSIILALYTIQLPSSRSLSSIATTIGALAKYSTFFGHKHHHHHHHHHHHHHY 65

Query: 67  ADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLKRNL 126
              E        C+ +  S L  +  T+ V  VD +GC NF+ VQ+A+D VP LS  + L
Sbjct: 66  HHHE----PIKCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTL 125

Query: 127 IWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIA 186
           I +NSG Y EKV V  +K N+  +G+GY  T+I WNDTA S+  T  S S  VF++NF A
Sbjct: 126 IIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTA 185

Query: 187 KNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQ 246
            NISF N AP P PG+  AQAVA+RI GD AAF+ CGF+GAQDTL DD+GRH+FK+C+IQ
Sbjct: 186 YNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQ 245

Query: 247 GSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVAG 306
           GSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC + G
Sbjct: 246 GSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDG 305

Query: 307 TGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTSA 366
           +G + LGRAW  ++ VVF NT M+ II PEGWN++ D  +++T+ +GE+ C G GA+   
Sbjct: 306 SGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKE 365

Query: 367 RAPFVQRLNDTQVSPFLNLSFIDADQWL 391
           R  F ++L D++ S F+++SFID D+WL
Sbjct: 366 RVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of MS002616 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.0e-88
Identity = 162/331 (48.94%), Postives = 214/331 (64.65%), Query Frame = 0


Query: 61  SPADEEDQAA-SSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPSLSLK 120
           SP +  DQ     S CD    S  PT+   T V  V  NGC  F  VQ A+DA    S  
Sbjct: 12  SPIESVDQRIHHPSKCDHL--SKFPTK-GFTMVLKVSLNGCGRFKRVQDAIDASIGSSQS 71

Query: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180
           + LI I+ GIY E+ +V  +K N+  +G GY+ T+I WN+T  SS+GTF S SV VF   
Sbjct: 72  KTLILIDFGIYRERFIVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEK 131

Query: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240
           F A NISF N AP P PG V AQAVA+++ GD AAF+ CGF+G QDTL D  GRH+FK C
Sbjct: 132 FTAYNISFKNTAPAPNPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGC 191

Query: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           +I+GSIDFIFGNGRS YE C L S+A       +   G +TA+G+ +  + +GF FVNC 
Sbjct: 192 FIEGSIDFIFGNGRSLYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFVNCK 251

Query: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + G+ RVWLGRAWRP++RV+F  T M+ +++ +GWND  DP   +T++YGE+ C G GAN
Sbjct: 252 ITGSARVWLGRAWRPYARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGAN 311

Query: 361 TSARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
            S R  + + L+D + +PF N+SFID ++WL
Sbjct: 312 HSKRVTYAKLLSDVEAAPFTNISFIDGEEWL 333

[Close]

BLAST of MS002616 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.4e-82
Identity = 145/295 (49.15%), Postives = 199/295 (67.46%), Query Frame = 0


Query: 102 NFTTVQAAVDAVPSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTA 161
           +FT +Q A+D++P ++  R +I +++G+Y EKV +P  K  +T EG+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 162 NSSH------GTFYSASVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAF 221
            +        GT+ SAS  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 222 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFV 281
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 282 NGAVTAHGRASADENSGFAFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWN 341
            GAVTA GR+S  E++GF+FV C V GTG ++LGRAW PFSRVVF  T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 342 DFNDPARDQTIFYGEYNCSGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWL 391
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +  PFL+L+FID  +W+
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

[Close]

BLAST of MS002616 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 4.3e-71
Identity = 149/347 (42.94%), Postives = 204/347 (58.79%), Query Frame = 0


Query: 53  HHHHRRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDA 112
           HHHHR+   P+  +                         V  V  NG   F +VQ AVD+
Sbjct: 46  HHHHRKWVGPSGHK-------------------------VITVSLNGHAQFRSVQDAVDS 105

Query: 113 VPSLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTAN--SSHG---- 172
           +P  + K   I I  G Y EKV+VP +K  +TF+G G   TAI W+D A+   ++G    
Sbjct: 106 IPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLR 165

Query: 173 TFYSASVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDT 232
           T+ +ASV V+++ F A+NISF N AP P PG  G QAVA RI GD A F  CGF+GAQDT
Sbjct: 166 TYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDT 225

Query: 233 LHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRAS 292
           L DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A      S+F  G++ AHGR  
Sbjct: 226 LCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSIAAHGRTC 285

Query: 293 ADENSGFAFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPA-RDQT 352
            +E +GFAFV C V GTG +++GRA   +SR+V+  T    ++A  GW+D++  + + +T
Sbjct: 286 PEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKT 345

Query: 353 IFYGEYNCSGAGANTSARAPFVQRLNDTQVSPFLNLSFIDADQWLHP 393
            F+G YNC G GA  +    + + L+     PF+  SF++   W+ P
Sbjct: 346 AFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

The following BLAST results are available for this feature:

BLAST of MS002616 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136420.1	1.8e-228	99.24	probable pectinesterase 8 [Momordica charantia]	[more]
XP_038903629.1	3.8e-194	84.75	probable pectinesterase 8 [Benincasa hispida]	[more]
XP_008451885.1	9.0e-188	82.16	PREDICTED: probable pectinesterase 8 [Cucumis melo]	[more]
XP_004147773.1	1.2e-187	82.44	probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa...	[more]
KAG7015100.1	6.7e-183	82.49	putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS002616 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23038	1.9e-148	64.27	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	4.2e-95	47.94	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q9ZQA4	4.2e-87	48.94	Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1	[more]
Q8VYZ3	2.0e-81	49.15	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	6.1e-70	42.94	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of MS002616 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C3U9	8.7e-229	99.24	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1	[more]
A0A1S3BRY7	4.3e-188	82.16	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1	[more]
A0A0A0KVA9	5.7e-188	82.44	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1	[more]
A0A6J1ETE2	6.1e-182	81.98	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1	[more]
A0A6J1J3X1	1.0e-181	81.98	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1	[more]

BLAST of MS002616 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05310.1	1.4e-149	64.27	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	3.0e-96	47.94	Pectin lyase-like superfamily protein	[more]
AT2G36700.1	3.0e-88	48.94	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	1.4e-82	49.15	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	4.3e-71	42.94	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 91..393 e-value: 7.2E-100 score: 336.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 95..386 e-value: 1.9E-70 score: 236.9
None	No IPR available	PANTHER	PTHR31321:SF33	PECTINESTERASE 8-RELATED	coord: 46..392
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 46..392
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 241..250
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 91..392

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002616.1	MS002616.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity