MS002523 (gene) Bitter gourd (TR) v1

BLAST of MS002523 vs. NCBI nr
Match: XP_022157511.1 (putative G3BP-like protein [Momordica charantia])

HSP 1 Score: 845.9 bits (2184), Expect = 1.6e-241
Identity = 437/439 (99.54%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE 130

Query: 131 EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP 190
           EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP
Sbjct: 131 EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP 190

Query: 191 EQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRAA 250
           EQQQQEEFETEVVVEEAPVEDLVTSHQNVV+SVQEPLSAVIDEPVGEPEKRTYASILRAA
Sbjct: 191 EQQQQEEFETEVVVEEAPVEDLVTSHQNVVDSVQEPLSAVIDEPVGEPEKRTYASILRAA 250

Query: 251 RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG 310
           RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG
Sbjct: 251 RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG 310

Query: 311 EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA 370
           EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA
Sbjct: 311 EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA 370

Query: 371 LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS 430
           LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS
Sbjct: 371 LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS 430

Query: 431 DYGRLRGNGFPQRGYHKVQ 450
           DYGRLRGNGFPQRGYHKVQ
Sbjct: 431 DYGRLRGNGFPQRGYHKVQ 449

BLAST of MS002523 vs. NCBI nr
Match: XP_038874349.1 (nuclear transport factor 2 [Benincasa hispida])

HSP 1 Score: 788.9 bits (2036), Expect = 2.3e-224
Identity = 405/440 (92.05%), Postives = 426/440 (96.82%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+GIRKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGIRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEAEL+A SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEAELSAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEPLSAVIDEP+GEPEKRTYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPLSAVIDEPIGEPEKRTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAESAQSAIPQPSFYP+A ATS+WNH PEPAPQQINP PSY PESGADT+EEG+GVEDE
Sbjct: 251 ARAESAQSAIPQPSFYPSAPATSEWNHIPEPAPQQINPVPSYAPESGADTMEEGYGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKTFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG+YQS+APRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRGSYQSEAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKVQ 450
           SDYGRLRGNGFPQRGYHKVQ
Sbjct: 431 SDYGRLRGNGFPQRGYHKVQ 450

BLAST of MS002523 vs. NCBI nr
Match: TYK24612.1 (putative G3BP-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 399/439 (90.89%), Postives = 422/439 (96.13%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+G+RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEA+LNA SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEADLNAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEP+SAV DEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPISAVNDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A ATSDWNH PEPAPQ +NPAPSY PE G DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSAPATSDWNHIPEPAPQHVNPAPSYAPEPGPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKV 449
           +DYGRLRGNGFPQRGYHKV
Sbjct: 431 NDYGRLRGNGFPQRGYHKV 449

BLAST of MS002523 vs. NCBI nr
Match: XP_008459963.1 (PREDICTED: putative G3BP-like protein [Cucumis melo] >XP_008459964.1 PREDICTED: putative G3BP-like protein [Cucumis melo])

HSP 1 Score: 779.2 bits (2011), Expect = 1.9e-221
Identity = 400/440 (90.91%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+G+RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEA+LNA SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEADLNAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEP+SAV DEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPISAVNDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A ATSDWNH PEPAPQ +NPAPSY PE   DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSAPATSDWNHIPEPAPQHVNPAPSYAPEPVPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKVQ 450
           SDYGRLRGNGFPQRGYHKVQ
Sbjct: 431 SDYGRLRGNGFPQRGYHKVQ 450

BLAST of MS002523 vs. NCBI nr
Match: XP_011656769.1 (nuclear transport factor 2 [Cucumis sativus] >KGN46374.1 hypothetical protein Csa_005441 [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 8.6e-219
Identity = 396/440 (90.00%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTL+MSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLVMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+ +RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNRMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QH+P+PVL+EN+FEA+LNA +SIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHSPLPVLTENKFEADLNAPNSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEPL AVIDEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPLPAVIDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A+ATSDWNH PEPAPQ +NPAPSY PE G DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSASATSDWNHIPEPAPQHVNPAPSYAPEPGPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI IAGRQVYIEERRPN +G RGGRRGR RG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIAGRQVYIEERRPN-NGARGGRRGRARGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKVQ 450
           SDYGRLRGNGFPQRGYHKVQ
Sbjct: 431 SDYGRLRGNGFPQRGYHKVQ 449

BLAST of MS002523 vs. ExPASy Swiss-Prot
Match: Q9FME2 (Nuclear transport factor 2 OS=Arabidopsis thaliana OX=3702 GN=NTF2 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-44
Identity = 160/458 (34.93%), Postives = 242/458 (52.84%), Query Frame = 0

Query: 13  VGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSS-ETASTMLQIHTLIMSLNFTAF 72
           VG  FVEQYYH+L Q P LVH+FY ++S + R D   +  T +TM  I+  I+SL +  +
Sbjct: 15  VGRAFVEQYYHILHQSPGLVHRFYQDSSFLTRPDVTGAVTTVTTMQAINDKILSLKYEDY 74

Query: 73  S--IKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMD 132
           +  I+T ++ +S   G++V+V+G     + +  +KF Q+FFLAPQ+KGYFVLND+F F++
Sbjct: 75  TAEIETADAQESHERGVIVLVTGRLTGND-NVRKKFSQSFFLAPQDKGYFVLNDVFRFLE 134

Query: 133 EE--ILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKY 192
           E+    Q   +P+   N    ++ A    P  P    V  E   E      IE++ +D  
Sbjct: 135 EKEVTAQARSVPI---NGTTRDVQA----PIEPERVVVSHEPEVEPEPVASIEEEDLD-- 194

Query: 193 SLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASIL 252
           ++ E     + +  VVV+  P+E       N + SV          P G+  K +YASIL
Sbjct: 195 NVAEVYDPSDKDEGVVVDVEPIEPPTQISHNEILSV----------PQGDAPKHSYASIL 254

Query: 253 RAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP--QQIN--PA-PSYVPESGA-DTIE 312
           +  ++       P P+       T    + P PAP  Q++   PA P+  PE+ A + + 
Sbjct: 255 KQMKSS------PAPT-------THVARNKPRPAPVNQKLTAPPAEPAARPEASAHENVP 314

Query: 313 EGFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEF 372
               V+ E +  S+YVRNLP      ++E+ FK FG I+ +G+ +RS K+ G C+ FVEF
Sbjct: 315 NSSHVDVEDDGHSIYVRNLPFDSTPTQLEEVFKNFGAIKHEGIQVRSNKQQGFCFGFVEF 374

Query: 373 EDIIGVQNALKASPIHIAGRQVYIEERRPNS--SGTRGGRRGR---GRGNYQSDA---PR 432
           E   G Q+AL+ASP+ I  RQ  +EE++ NS   G  GG RGR   GRG++++++    R
Sbjct: 375 ETSSGKQSALEASPVTIGDRQAVVEEKKTNSRGGGNNGGSRGRYFSGRGSFRNESFKGGR 434

Query: 433 GRFGSRNLGRG----SSQDGSDYGRLRGNGF---PQRG 445
           G  G    GRG    S +  S   R  G G+   PQ G
Sbjct: 435 GGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNG 439

BLAST of MS002523 vs. ExPASy Swiss-Prot
Match: O94260 (Putative G3BP-like protein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nxt3 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.8e-17
Identity = 113/444 (25.45%), Postives = 194/444 (43.69%), Query Frame = 0

Query: 12  QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVD-GDSSETASTMLQIHTLIMSLNF-- 71
           ++G  FV++YY  L ++P+ +H FY++ S++I  D G+S        +IH  I+ L+F  
Sbjct: 17  EIGWMFVQEYYTYLNKEPNRLHCFYTKKSTLIHGDEGESISLCHGQQEIHNKILDLDFQN 76

Query: 72  TAFSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM 131
               I  ++S+ S NGGI++ V G   +K     RKF QTFFLA Q  GYFVLNDIF F+
Sbjct: 77  CKVLISNVDSLASSNGGIVIQVLGEMSNKGKLS-RKFAQTFFLAEQPNGYFVLNDIFRFL 136

Query: 132 DEEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYS 191
            E++ +    P   E + +                   + ++  YV+ V  ++       
Sbjct: 137 REDVEEEEESPDAVEKEKK-------------------DVASEPYVNGVQSQE------H 196

Query: 192 LPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILR 251
           LP  +++  ++     E       + S  N  +S+ +   AV +EPV +  + +  S + 
Sbjct: 197 LPSAKEEGHYQDPAATENNFATAALIS--NETDSLNQATLAVPEEPVIQVTEASVPSFVS 256

Query: 252 AARAESAQSAIPQPSFYPNAAATSD---------W------NHTPEPAPQQINPAPSYVP 311
               +    A+   S   +A   SD         W      NH    +   ++   S   
Sbjct: 257 QQENQLQDEALTSNSKNADAIGASDANVATAPKSWADLIARNHPDVKSQASVSSTASTTG 316

Query: 312 ES----GADTIEE---GFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFI 371
           ++     AD  ++    +   +E    SV+V+N+PP  ++  ++     FG ++     I
Sbjct: 317 QTVKGVNADQTQQPTAPYTQSNELLETSVFVKNIPPETSDVSLKSAMSIFGPVKA----I 376

Query: 372 RSRKEIGVCYAFVEFEDIIGVQNALKASPIHIAGRQVYIEERRPNSSG--TRGGRRGRGR 429
              +  G   A+V+F +   VQ AL    + I    + IEERR   SG   + G + +  
Sbjct: 377 EFARRKGT--AYVDFVNHECVQLALNKKTLQINNATLNIEERRRLFSGKFNKSGDK-KSN 425

BLAST of MS002523 vs. ExPASy Swiss-Prot
Match: Q32LC7 (Ras GTPase-activating protein-binding protein 1 OS=Bos taurus OX=9913 GN=G3BP1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.6e-16
Identity = 119/447 (26.62%), Postives = 199/447 (44.52%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIR----VDGDSSETASTMLQIHTLIMSL 70
           L VG  FV QYY +L Q PD++H+FY + SS +      +G  ++      +IH  +MS 
Sbjct: 9   LLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQ 68

Query: 71  NFT--AFSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQ---EKGYFVL 130
           NFT     I+ +++  + N G++V V G   S     +R+F+QTF LAP+      ++V 
Sbjct: 69  NFTNCHTKIRHVDAHATLNDGVVVQVMG-LLSNNNQALRRFMQTFVLAPEGSVANKFYVH 128

Query: 131 NDIFHFMDEEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSV---- 190
           NDIF + DE          ++E Q E+E             + V ++S   Y  +V    
Sbjct: 129 NDIFRYQDEVF-----GGFITEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQTVSNDL 188

Query: 191 --HIEDDPVDKYSLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPV 250
             H+E+   +    PE + ++E  +EV  E++      T+ ++V  S   P  A I + V
Sbjct: 189 EEHLEEPVAEPEPEPEPEPEQEPVSEVQEEKSEPVLEETAPEDVQKS-SSPAPADIAQTV 248

Query: 251 GEPEKRTYASILRAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP------------- 310
            E + RT++     ++      A+P     P+          PE  P             
Sbjct: 249 QE-DLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPLQRPQRDQ 308

Query: 311 ----QQINPAPSYVPESGADTIEEGFGVEDEGEIK-----SVYVRNLPPSVNEAEIEQEF 370
               Q+IN  P   P    +  E+G  VE    ++      +++ NLP  V+++E++  F
Sbjct: 309 RVREQRINVPPQRGPRPVREAGEQG-DVEPRRIVRHPDSHQLFIGNLPHEVDKSELKDFF 368

Query: 371 KAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNALKASPIHIAGR-QVYIEERRPNS 418
           + +G +    + I S  ++   + FV F+D   VQ  L   PI   G  ++ +EE++  +
Sbjct: 369 QNYGNVVE--LRINSGGKL-PNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRA 428

BLAST of MS002523 vs. ExPASy Swiss-Prot
Match: Q13283 (Ras GTPase-activating protein-binding protein 1 OS=Homo sapiens OX=9606 GN=G3BP1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.2e-15
Identity = 123/470 (26.17%), Postives = 203/470 (43.19%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIR----VDGDSSETASTMLQIHTLIMSL 70
           L VG  FV QYY +L Q PD++H+FY + SS +      +G  ++      +IH  +MS 
Sbjct: 9   LLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQ 68

Query: 71  NFT--AFSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQ---EKGYFVL 130
           NFT     I+ +++  + N G++V V G   S     +R+F+QTF LAP+      ++V 
Sbjct: 69  NFTNCHTKIRHVDAHATLNDGVVVQVMG-LLSNNNQALRRFMQTFVLAPEGSVANKFYVH 128

Query: 131 NDIFHFMDEEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSV---- 190
           NDIF + DE          ++E Q E+E             + V ++S   Y  +V    
Sbjct: 129 NDIFRYQDEVF-----GGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSND 188

Query: 191 ---HIEDDPVDKYSLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEP 250
              H+E+   +    PE + ++E  +E + EE P   L  +          P  A I + 
Sbjct: 189 MEEHLEEPVAEPEPDPEPEPEQEPVSE-IQEEKPEPVLEETAPEDAQKSSSPAPADIAQT 248

Query: 251 VGEPEKRTYASILRAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP------------ 310
           V E + RT++     ++      A+P     P+          PE  P            
Sbjct: 249 VQE-DLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD 308

Query: 311 -----QQINPAPSYVPESGADTIEEGFGVEDEGEIK-----SVYVRNLPPSVNEAEIEQE 370
                Q+IN  P   P    +  E+G  +E    ++      +++ NLP  V+++E++  
Sbjct: 309 QRVREQRINIPPQRGPRPIREAGEQG-DIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDF 368

Query: 371 FKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNALKASPIHIAGR-QVYIEERRPN 430
           F+++G +    + I S  ++   + FV F+D   VQ  L   PI   G  ++ +EE++  
Sbjct: 369 FQSYGNVVE--LRINSGGKL-PNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTR 428

Query: 431 SS--GTRGGRRGRGRGNYQSDAPRGRFGS--RNLGRGSSQDGSDYGRLRG 438
           ++  G R   R RG G      PRG  G   R   RG       +G  RG
Sbjct: 429 AAREGDRRDNRLRGPG-----GPRGGLGGGMRGPPRGGMVQKPGFGVGRG 461

BLAST of MS002523 vs. ExPASy Swiss-Prot
Match: Q5RB87 (Ras GTPase-activating protein-binding protein 1 OS=Pongo abelii OX=9601 GN=G3BP1 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.9e-15
Identity = 123/470 (26.17%), Postives = 203/470 (43.19%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIR----VDGDSSETASTMLQIHTLIMSL 70
           L VG  FV QYY +L Q PD++H+FY + SS +      +G  ++      +IH  +MS 
Sbjct: 9   LLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQ 68

Query: 71  NFT--AFSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQ---EKGYFVL 130
           NFT     I+ +++  + N G++V V G   S     +R+F+QTF LAP+      ++V 
Sbjct: 69  NFTNCHTKIRHVDAHATLNDGVVVQVMG-LLSNNNQALRRFMQTFVLAPEGSVANKFYVH 128

Query: 131 NDIFHFMDEEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSV---- 190
           NDIF + DE          ++E Q E+E             + V ++S   Y  +V    
Sbjct: 129 NDIFRYQDEVF-----GGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSND 188

Query: 191 ---HIEDDPVDKYSLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEP 250
              H+E+   +    PE + ++E  +E + EE P   L  +          P  A I + 
Sbjct: 189 MEEHLEEPVAEPEPDPEPEPEQEPVSE-IQEEKPEPVLEETVPEDAQKSSSPAPADIAQT 248

Query: 251 VGEPEKRTYASILRAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP------------ 310
           V E + RT++     ++      A+P     P+          PE  P            
Sbjct: 249 VQE-DLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD 308

Query: 311 -----QQINPAPSYVPESGADTIEEGFGVEDEGEIK-----SVYVRNLPPSVNEAEIEQE 370
                Q+IN  P   P    +  E+G  +E    ++      +++ NLP  V+++E++  
Sbjct: 309 QRVREQRINIPPQRGPRPIREAGEQG-DIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDF 368

Query: 371 FKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNALKASPIHIAGR-QVYIEERRPN 430
           F+++G +    + I S  ++   + FV F+D   VQ  L   PI   G  ++ +EE++  
Sbjct: 369 FQSYGNVVE--LRINSGGKL-PNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTR 428

Query: 431 SS--GTRGGRRGRGRGNYQSDAPRGRFGS--RNLGRGSSQDGSDYGRLRG 438
           ++  G R   R RG G      PRG  G   R   RG       +G  RG
Sbjct: 429 AAREGDRRDNRLRGPG-----GPRGGLGGGMRGPPRGGMVQKPGFGVGRG 461

BLAST of MS002523 vs. ExPASy TrEMBL
Match: A0A6J1DYE2 (putative G3BP-like protein OS=Momordica charantia OX=3673 GN=LOC111024190 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.8e-242
Identity = 437/439 (99.54%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDE 130

Query: 131 EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP 190
           EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP
Sbjct: 131 EILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLP 190

Query: 191 EQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRAA 250
           EQQQQEEFETEVVVEEAPVEDLVTSHQNVV+SVQEPLSAVIDEPVGEPEKRTYASILRAA
Sbjct: 191 EQQQQEEFETEVVVEEAPVEDLVTSHQNVVDSVQEPLSAVIDEPVGEPEKRTYASILRAA 250

Query: 251 RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG 310
           RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG
Sbjct: 251 RAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDEG 310

Query: 311 EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA 370
           EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA
Sbjct: 311 EIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQNA 370

Query: 371 LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS 430
           LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS
Sbjct: 371 LKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDGS 430

Query: 431 DYGRLRGNGFPQRGYHKVQ 450
           DYGRLRGNGFPQRGYHKVQ
Sbjct: 431 DYGRLRGNGFPQRGYHKVQ 449

BLAST of MS002523 vs. ExPASy TrEMBL
Match: A0A5D3DM71 (Putative G3BP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001590 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.3e-222
Identity = 399/439 (90.89%), Postives = 422/439 (96.13%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+G+RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEA+LNA SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEADLNAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEP+SAV DEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPISAVNDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A ATSDWNH PEPAPQ +NPAPSY PE G DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSAPATSDWNHIPEPAPQHVNPAPSYAPEPGPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKV 449
           +DYGRLRGNGFPQRGYHKV
Sbjct: 431 NDYGRLRGNGFPQRGYHKV 449

BLAST of MS002523 vs. ExPASy TrEMBL
Match: A0A1S3CBV7 (putative G3BP-like protein OS=Cucumis melo OX=3656 GN=LOC103498922 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 9.0e-222
Identity = 400/440 (90.91%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+G+RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEA+LNA SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEADLNAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEP+SAV DEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPISAVNDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A ATSDWNH PEPAPQ +NPAPSY PE   DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSAPATSDWNHIPEPAPQHVNPAPSYAPEPVPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKVQ 450
           SDYGRLRGNGFPQRGYHKVQ
Sbjct: 431 SDYGRLRGNGFPQRGYHKVQ 450

BLAST of MS002523 vs. ExPASy TrEMBL
Match: A0A0A0KBZ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G088020 PE=4 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.2e-219
Identity = 396/440 (90.00%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTL+MSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLVMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+ +RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNRMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QH+P+PVL+EN+FEA+LNA +SIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHSPLPVLTENKFEADLNAPNSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEPL AVIDEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPLPAVIDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A+ATSDWNH PEPAPQ +NPAPSY PE G DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSASATSDWNHIPEPAPQHVNPAPSYAPEPGPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEFEDIIGVQN 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRKEIGVCYAFVEFEDI+GVQN
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKEIGVCYAFVEFEDILGVQN 370

Query: 371 ALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRGNYQSDAPRGRFGSRNLGRGSSQDG 430
           ALKASPI IAGRQVYIEERRPN +G RGGRRGR RG+YQSDAPRGRFGSR+LGRGSSQDG
Sbjct: 371 ALKASPIQIAGRQVYIEERRPN-NGARGGRRGRARGSYQSDAPRGRFGSRSLGRGSSQDG 430

Query: 431 SDYGRLRGNGFPQRGYHKVQ 450
           SDYGRLRGNGFPQRGYHKVQ
Sbjct: 431 SDYGRLRGNGFPQRGYHKVQ 449

BLAST of MS002523 vs. ExPASy TrEMBL
Match: A0A5A7T9E4 (Putative G3BP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G001670 PE=4 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.0e-218
Identity = 399/463 (86.18%), Postives = 422/463 (91.14%), Query Frame = 0

Query: 11  LQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70
           +QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA
Sbjct: 11  VQVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTA 70

Query: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFM-D 130
           FSIKTINSMDSWNGGILVVVSGSAKSKEF+G+RKFVQTFFLAPQEKGYFVLNDIFHF+ +
Sbjct: 71  FSIKTINSMDSWNGGILVVVSGSAKSKEFNGMRKFVQTFFLAPQEKGYFVLNDIFHFIEE 130

Query: 131 EEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSL 190
           EEI+QHNP+PV+SEN+FEA+LNA SSIP+PPVSDYVLEE+AREYVDSVHIEDDPVDKYSL
Sbjct: 131 EEIVQHNPLPVISENKFEADLNAPSSIPEPPVSDYVLEENAREYVDSVHIEDDPVDKYSL 190

Query: 191 PEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILRA 250
           PEQQQQEEFE+EVVVEEAPVEDLV SHQNVVNSVQEP+SAV DEP+GEPEK+TYASILRA
Sbjct: 191 PEQQQQEEFESEVVVEEAPVEDLVASHQNVVNSVQEPISAVNDEPIGEPEKKTYASILRA 250

Query: 251 ARAESAQSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESGADTIEEGFGVEDE 310
           ARAE+AQSAIPQPSFYP+A ATSDWNH PEPAPQ +NPAPSY PE G DTIEEGFGVEDE
Sbjct: 251 ARAEAAQSAIPQPSFYPSAPATSDWNHIPEPAPQHVNPAPSYAPEPGPDTIEEGFGVEDE 310

Query: 311 GEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRK------------------- 370
           GE KSVYVRNLPPSV EAEIEQEFK FGRI PDGVFIRSRK                   
Sbjct: 311 GEPKSVYVRNLPPSVTEAEIEQEFKDFGRILPDGVFIRSRKVSTLFFDVCWLIFTGWLNI 370

Query: 371 -----EIGVCYAFVEFEDIIGVQNALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRGRG 430
                EIGVCYAFVEFEDI+GVQNALKASPI I GRQVYIEERRPNSSG RGGRRGRGRG
Sbjct: 371 YFFVQEIGVCYAFVEFEDILGVQNALKASPIQIGGRQVYIEERRPNSSGARGGRRGRGRG 430

Query: 431 NYQSDAPRGRFGSRNLGRGSSQDGSDYGRLRGNGFPQRGYHKV 449
           +YQSDAPRGRFGSR+LGRGSSQDG+DYGRLRGNGFPQRGYHKV
Sbjct: 431 SYQSDAPRGRFGSRSLGRGSSQDGNDYGRLRGNGFPQRGYHKV 473

BLAST of MS002523 vs. TAIR 10
Match: AT5G43960.1 (Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain )

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-125
Identity = 257/449 (57.24%), Postives = 329/449 (73.27%), Query Frame = 0

Query: 12  QVGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSSETASTMLQIHTLIMSLNFTAF 71
           QVGSYFV QYY VL+QQPDL+HQFYSE S  IR+DGDS+ETA+++L IH ++MSLNFTA 
Sbjct: 10  QVGSYFVGQYYQVLQQQPDLIHQFYSEPSRAIRIDGDSTETANSLLHIHNMVMSLNFTAI 69

Query: 72  SIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMDEE 131
            +KTINS++SW GG+LVVVSGS K+KEFS  R FVQTFFLAPQEKGYFVL+D+F F+DE 
Sbjct: 70  EVKTINSVESWEGGVLVVVSGSVKTKEFSNRRSFVQTFFLAPQEKGYFVLSDVFLFVDEG 129

Query: 132 ILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKYSLPE 191
            + ++    LSE + EA+LN  +  PDP VSDYVLEE A +YV++V I+DD VDKYSL E
Sbjct: 130 TVYYHQPSYLSEIKHEAQLNPPTRHPDPQVSDYVLEEEASDYVNAVQIKDDLVDKYSLQE 189

Query: 192 QQ---QQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASILR 251
            Q   Q E++E EV +EE P E++       V+ V E  +A ++EPVGE  K +YASIL+
Sbjct: 190 DQHQPQHEDYEDEVAIEETPREEV------AVDVVHEHRAAPVEEPVGEKSKMSYASILK 249

Query: 252 AAR-AESAQSAIPQPSFYPNAAATSDWN---HTPEP------APQQINPAPSYVPESGAD 311
            A+ A +   A  QPS+  ++   ++W+    TP P      AP Q + + +YV + GA+
Sbjct: 250 VAKEAATVPVAATQPSYNKSSQDINEWDQPMRTPSPQLAAPLAPIQQSNSSTYVSDYGAE 309

Query: 312 TIEEGFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEI-GVCYA 371
             E+G G ED  E KSVYVRNLP  ++ +EIE+EFK FG I+PDGVF+R+RK++ GVCYA
Sbjct: 310 A-EDGSGFED-FEFKSVYVRNLPSDISASEIEEEFKNFGTIKPDGVFLRTRKDVMGVCYA 369

Query: 372 FVEFEDIIGVQNALKASPIHIAGRQVYIEERRPNSSGTRGGRR--GRGRGNYQSDAPRGR 431
           FVEFED+  V+NA+KASPI++ GRQVYIEERRPN +G RG RR  GRGRG Y ++APRGR
Sbjct: 370 FVEFEDMTSVENAIKASPIYLGGRQVYIEERRPNPAGVRGARRGGGRGRGGYPTEAPRGR 429

Query: 432 FGSRNLGRGSSQDGSDYGRLRGNGFPQRG 445
           FG R  GRG +QDG DY R RGNG+ + G
Sbjct: 430 FGGRGSGRG-NQDGGDY-RPRGNGYYRGG 448

BLAST of MS002523 vs. TAIR 10
Match: AT5G43960.2 (Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain )

HSP 1 Score: 374.4 bits (960), Expect = 1.3e-103
Identity = 221/399 (55.39%), Postives = 286/399 (71.68%), Query Frame = 0

Query: 62  LIMSLNFTAFSIKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVL 121
           ++MSLNFTA  +KTINS++SW GG+LVVVSGS K+KEFS  R FVQTFFLAPQEKGYFVL
Sbjct: 1   MVMSLNFTAIEVKTINSVESWEGGVLVVVSGSVKTKEFSNRRSFVQTFFLAPQEKGYFVL 60

Query: 122 NDIFHFMDEEILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIED 181
           +D+F F+DE  + ++    LSE + EA+LN  +  PDP VSDYVLEE A +YV++V I+D
Sbjct: 61  SDVFLFVDEGTVYYHQPSYLSEIKHEAQLNPPTRHPDPQVSDYVLEEEASDYVNAVQIKD 120

Query: 182 DPVDKYSLPEQQ---QQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEP 241
           D VDKYSL E Q   Q E++E EV +EE P E++       V+ V E  +A ++EPVGE 
Sbjct: 121 DLVDKYSLQEDQHQPQHEDYEDEVAIEETPREEV------AVDVVHEHRAAPVEEPVGEK 180

Query: 242 EKRTYASILRAAR-AESAQSAIPQPSFYPNAAATSDWN---HTPEP------APQQINPA 301
            K +YASIL+ A+ A +   A  QPS+  ++   ++W+    TP P      AP Q + +
Sbjct: 181 SKMSYASILKVAKEAATVPVAATQPSYNKSSQDINEWDQPMRTPSPQLAAPLAPIQQSNS 240

Query: 302 PSYVPESGADTIEEGFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRS 361
            +YV + GA+  E+G G ED  E KSVYVRNLP  ++ +EIE+EFK FG I+PDGVF+R+
Sbjct: 241 STYVSDYGAEA-EDGSGFED-FEFKSVYVRNLPSDISASEIEEEFKNFGTIKPDGVFLRT 300

Query: 362 RKEI-GVCYAFVEFEDIIGVQNALKASPIHIAGRQVYIEERRPNSSGTRGGRR--GRGRG 421
           RK++ GVCYAFVEFED+  V+NA+KASPI++ GRQVYIEERRPN +G RG RR  GRGRG
Sbjct: 301 RKDVMGVCYAFVEFEDMTSVENAIKASPIYLGGRQVYIEERRPNPAGVRGARRGGGRGRG 360

Query: 422 NYQSDAPRGRFGSRNLGRGSSQDGSDYGRLRGNGFPQRG 445
            Y ++APRGRFG R  GRG +QDG DY R RGNG+ + G
Sbjct: 361 GYPTEAPRGRFGGRGSGRG-NQDGGDY-RPRGNGYYRGG 389

BLAST of MS002523 vs. TAIR 10
Match: AT5G60980.2 (Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain )

HSP 1 Score: 182.2 bits (461), Expect = 9.3e-46
Identity = 160/458 (34.93%), Postives = 242/458 (52.84%), Query Frame = 0

Query: 13  VGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSS-ETASTMLQIHTLIMSLNFTAF 72
           VG  FVEQYYH+L Q P LVH+FY ++S + R D   +  T +TM  I+  I+SL +  +
Sbjct: 15  VGRAFVEQYYHILHQSPGLVHRFYQDSSFLTRPDVTGAVTTVTTMQAINDKILSLKYEDY 74

Query: 73  S--IKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMD 132
           +  I+T ++ +S   G++V+V+G     + +  +KF Q+FFLAPQ+KGYFVLND+F F++
Sbjct: 75  TAEIETADAQESHERGVIVLVTGRLTGND-NVRKKFSQSFFLAPQDKGYFVLNDVFRFLE 134

Query: 133 EE--ILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKY 192
           E+    Q   +P+   N    ++ A    P  P    V  E   E      IE++ +D  
Sbjct: 135 EKEVTAQARSVPI---NGTTRDVQA----PIEPERVVVSHEPEVEPEPVASIEEEDLD-- 194

Query: 193 SLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASIL 252
           ++ E     + +  VVV+  P+E       N + SV          P G+  K +YASIL
Sbjct: 195 NVAEVYDPSDKDEGVVVDVEPIEPPTQISHNEILSV----------PQGDAPKHSYASIL 254

Query: 253 RAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP--QQIN--PA-PSYVPESGA-DTIE 312
           +  ++       P P+       T    + P PAP  Q++   PA P+  PE+ A + + 
Sbjct: 255 KQMKSS------PAPT-------THVARNKPRPAPVNQKLTAPPAEPAARPEASAHENVP 314

Query: 313 EGFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEF 372
               V+ E +  S+YVRNLP      ++E+ FK FG I+ +G+ +RS K+ G C+ FVEF
Sbjct: 315 NSSHVDVEDDGHSIYVRNLPFDSTPTQLEEVFKNFGAIKHEGIQVRSNKQQGFCFGFVEF 374

Query: 373 EDIIGVQNALKASPIHIAGRQVYIEERRPNS--SGTRGGRRGR---GRGNYQSDA---PR 432
           E   G Q+AL+ASP+ I  RQ  +EE++ NS   G  GG RGR   GRG++++++    R
Sbjct: 375 ETSSGKQSALEASPVTIGDRQAVVEEKKTNSRGGGNNGGSRGRYFSGRGSFRNESFKGGR 434

Query: 433 GRFGSRNLGRG----SSQDGSDYGRLRGNGF---PQRG 445
           G  G    GRG    S +  S   R  G G+   PQ G
Sbjct: 435 GGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNG 439

BLAST of MS002523 vs. TAIR 10
Match: AT5G60980.1 (Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain )

HSP 1 Score: 179.1 bits (453), Expect = 7.9e-45
Identity = 160/458 (34.93%), Postives = 242/458 (52.84%), Query Frame = 0

Query: 13  VGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDSS-ETASTMLQIHTLIMSLNFTAF 72
           VG  FVEQYYH+L Q P LVH+FY ++S + R D   +  T +TM  I+  I+SL +  +
Sbjct: 15  VGRAFVEQYYHILHQSPGLVHRFYQDSSFLTRPDVTGAVTTVTTMQAINDKILSLKYEDY 74

Query: 73  S--IKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMD 132
           +  I+T ++ +S   G++V+V+G     + +  +KF Q+FFLAPQ+KGYFVLND+F F++
Sbjct: 75  TAEIETADAQESHERGVIVLVTGRLTGND-NVRKKFSQSFFLAPQDKGYFVLNDVFRFLE 134

Query: 133 EE--ILQHNPMPVLSENQFEAELNASSSIPDPPVSDYVLEESAREYVDSVHIEDDPVDKY 192
           E+    Q   +P+   N    ++ A    P  P    V  E   E      IE++ +D  
Sbjct: 135 EKEVTAQARSVPI---NGTTRDVQA----PIEPERVVVSHEPEVEPEPVASIEEEDLD-- 194

Query: 193 SLPEQQQQEEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGEPEKRTYASIL 252
           ++ E     + +  VVV+  P+E       N + SV          P G+  K +YASIL
Sbjct: 195 NVAEVYDPSDKDEGVVVDVEPIEPPTQISHNEILSV----------PQGDAPKHSYASIL 254

Query: 253 RAARAESAQSAIPQPSFYPNAAATSDWNHTPEPAP--QQIN--PA-PSYVPESGA-DTIE 312
           +  ++       P P+       T    + P PAP  Q++   PA P+  PE+ A + + 
Sbjct: 255 KQMKSS------PAPT-------THVARNKPRPAPVNQKLTAPPAEPAARPEASAHENVP 314

Query: 313 EGFGVEDEGEIKSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVCYAFVEF 372
               V+ E +  S+YVRNLP      ++E+ FK FG I+ +G+ +RS K+ G C+ FVEF
Sbjct: 315 NSSHVDVEDDGHSIYVRNLPFDSTPTQLEEVFKNFGAIKHEGIQVRSNKQ-GFCFGFVEF 374

Query: 373 EDIIGVQNALKASPIHIAGRQVYIEERRPNS--SGTRGGRRGR---GRGNYQSDA---PR 432
           E   G Q+AL+ASP+ I  RQ  +EE++ NS   G  GG RGR   GRG++++++    R
Sbjct: 375 ETSSGKQSALEASPVTIGDRQAVVEEKKTNSRGGGNNGGSRGRYFSGRGSFRNESFKGGR 434

Query: 433 GRFGSRNLGRG----SSQDGSDYGRLRGNGF---PQRG 445
           G  G    GRG    S +  S   R  G G+   PQ G
Sbjct: 435 GGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNG 438

BLAST of MS002523 vs. TAIR 10
Match: AT3G25150.1 (Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain )

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-44
Identity = 160/446 (35.87%), Postives = 229/446 (51.35%), Query Frame = 0

Query: 13  VGSYFVEQYYHVLRQQPDLVHQFYSEASSMIRVDGDS-SETASTMLQIHTLIMSLNFTAF 72
           VG+ FV QYYH+L Q P+ VH+FY E S + R + +      ST+  I   IM+L +   
Sbjct: 19  VGNAFVPQYYHILHQSPEHVHRFYQEISKLGRPEENGLMSITSTLQAIDKKIMALGYGVI 78

Query: 73  S--IKTINSMDSWNGGILVVVSGSAKSKEFSGIRKFVQTFFLAPQEKGYFVLNDIFHFMD 132
           S  I T+++ +S  GG +V+V+G    K+ S  R F QTFFLAPQE GYFVLND+F F+D
Sbjct: 79  SAEIATVDTQESHGGGYIVLVTGYLTGKD-SVRRTFSQTFFLAPQETGYFVLNDMFRFID 138

Query: 133 EEILQH-NPMPVLSENQFEAELN------ASSSIPDPPVSD-YVLEESAREYVDSVHIED 192
           E  + H N +PV   N  +A +N      A+  IPD  V + YV E  A +  + +    
Sbjct: 139 EGTVVHGNQIPV---NNVQAPVNTYQDTAAAKEIPDDFVQEKYVQENHAVKQTEVLSKSI 198

Query: 193 DPVDKYSLPEQQQQ----EEFETEVVVEEAPVEDLVTSHQNVVNSVQEPLSAVIDEPVGE 252
           +  +K   P + +Q    EE      V EAP+E         V  V E      D   GE
Sbjct: 199 NEPEKVFTPSEDEQVSAAEEALVTETVNEAPIE---------VQKVGES-----DSRTGE 258

Query: 253 PEKRTYASILRAARAESA-QSAIPQPSFYPNAAATSDWNHTPEPAPQQINPAPSYVPESG 312
             KR+YASI++  +  +A  SA   P+            H P P P       S   +SG
Sbjct: 259 IPKRSYASIVKVMKENAAPMSASRTPTKVEPKKQEDQAIHIPLPTPL------SEKSDSG 318

Query: 313 ADTIEEGFGVEDEGEI-KSVYVRNLPPSVNEAEIEQEFKAFGRIQPDGVFIRSRKEIGVC 372
           A+        E+E  +  S+Y++ LP     A +E EF+ FG I+ +G+ +RS+K  G C
Sbjct: 319 ANVAVNENNQENERALGPSIYLKGLPLDATPALLENEFQKFGLIRTNGIQVRSQK--GFC 378

Query: 373 YAFVEFEDIIGVQNALKASPIHIAGRQVYIEERRPNSSGTRGGRRGRG-RGNYQSDAPRG 432
           + FVEFE    +Q+A++ASP+ + G +V +EE+R  + G   GR   G    Y+++  RG
Sbjct: 379 FGFVEFESASSMQSAIEASPVMLNGHKVVVEEKRSTARGNYRGRSTFGVNTGYRNEGGRG 437

Query: 433 RFGSRNLGRG--SSQDGSDYGRLRGN 439
           R GS   GRG     D + YG  RGN
Sbjct: 439 R-GSFGGGRGGYGRTDFNGYGNNRGN 437