MS002513 (gene) Bitter gourd (TR) v1

Overview


Name	MS002513
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cupin_3 domain-containing protein
Location	scaffold318: 698219 .. 698638 (-)
RNA-Seq Expression	MS002513
Synteny	MS002513

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGATGTATAACAGATCCATGGCGGACCAAGAACCCGACAACAACGACCTTAGAATAGTAGTAGAAAGCAAGCCTTCTGAAGCAAAGCTCCGAGAACTCAACATCAGCGGTTGGCCAAAGTAAGTACAATTTAATCTATGAGTTTAAGCTTTTGAGTTTAGTAGTAGTTAGAAAATTTTATGTACGCGCGTTTTCAGATGGGGGTGTTCGCCGGGGAAATACCAGCTGAAATTTGAGGCAGAAGAGACTTGCTATCTGGTGAAGGGGAAAGTGAAGGCATATTATCAAAAGGGAGGTTCCAATTGTGAACAATACACAGAGTTTGGCGCTGGGGATTTGGTCACCATTCCTAAAGGCCTCAGCTGCACTTGGGATGTCTCTGTTGCTGTTGATAAGTTCTACAGATTTCAATCTCCTTCC

mRNA sequence

CAGATGTATAACAGATCCATGGCGGACCAAGAACCCGACAACAACGACCTTAGAATAGTAGTAGAAAGCAAGCCTTCTGAAGCAAAGCTCCGAGAACTCAACATCAGCGGTTGGCCAAAATGGGGGTGTTCGCCGGGGAAATACCAGCTGAAATTTGAGGCAGAAGAGACTTGCTATCTGGTGAAGGGGAAAGTGAAGGCATATTATCAAAAGGGAGGTTCCAATTGTGAACAATACACAGAGTTTGGCGCTGGGGATTTGGTCACCATTCCTAAAGGCCTCAGCTGCACTTGGGATGTCTCTGTTGCTGTTGATAAGTTCTACAGATTTCAATCTCCTTCC

Coding sequence (CDS)

CAGATGTATAACAGATCCATGGCGGACCAAGAACCCGACAACAACGACCTTAGAATAGTAGTAGAAAGCAAGCCTTCTGAAGCAAAGCTCCGAGAACTCAACATCAGCGGTTGGCCAAAATGGGGGTGTTCGCCGGGGAAATACCAGCTGAAATTTGAGGCAGAAGAGACTTGCTATCTGGTGAAGGGGAAAGTGAAGGCATATTATCAAAAGGGAGGTTCCAATTGTGAACAATACACAGAGTTTGGCGCTGGGGATTTGGTCACCATTCCTAAAGGCCTCAGCTGCACTTGGGATGTCTCTGTTGCTGTTGATAAGTTCTACAGATTTCAATCTCCTTCC

Protein sequence

QMYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS

Homology

[Close]

BLAST of MS002513 vs. NCBI nr
Match: XP_022157536.1 (uncharacterized protein LOC111024205 [Momordica charantia])

HSP 1 Score: 240.4 bits (612), Expect = 7.9e-60
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV
Sbjct: 1   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQS S
Sbjct: 61  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSSS 113

[Close]

BLAST of MS002513 vs. NCBI nr
Match: XP_038906875.1 (uncharacterized protein LOC120092760 [Benincasa hispida])

HSP 1 Score: 175.3 bits (443), Expect = 3.1e-40
Identity = 83/116 (71.55%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 4   NRSMADQEPD-NNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVK 63
           N    ++ P  NN+L+I+VE  PS+AKL +LNI GWPKWGCS GKYQLKFEAEETCYLVK
Sbjct: 4   NEEEGNKNPSTNNNLKIIVERNPSQAKLSQLNIHGWPKWGCSAGKYQLKFEAEETCYLVK 63

Query: 64  GKVKAYYQ----KGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           GKVKAY +       S+CE+YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+S S
Sbjct: 64  GKVKAYPKGIDSSSSSSCEEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESQS 119

[Close]

BLAST of MS002513 vs. NCBI nr
Match: XP_023005099.1 (uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005100.1 uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005101.1 uncharacterized protein LOC111498188 [Cucurbita maxima])

HSP 1 Score: 173.7 bits (439), Expect = 9.0e-40
Identity = 84/113 (74.34%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           M N       P  N+LRI+VE  PSEAKL  LNI  WPKWGCS GKYQLKFEAEETCYLV
Sbjct: 1   MANEEGNSNNPSTNNLRIIVERNPSEAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAY +  GS   +YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+SPS
Sbjct: 61  KGKVKAYAK--GSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of MS002513 vs. NCBI nr
Match: KAG7025258.1 (hypothetical protein SDJN02_11753 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 172.9 bits (437), Expect = 1.5e-39
Identity = 83/113 (73.45%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           M N       P  N+LRI+VE  PS+AKL  LNI  WPKWGCS GKYQLKFEAEETCYLV
Sbjct: 1   MANEEGNSNNPSTNNLRIIVERNPSQAKLSMLNIQSWPKWGCSAGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAY +  GS   +YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+SPS
Sbjct: 61  KGKVKAYAK--GSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of MS002513 vs. NCBI nr
Match: KAG6592849.1 (hypothetical protein SDJN03_12325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 172.6 bits (436), Expect = 2.0e-39
Identity = 82/113 (72.57%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           M N       P  N+LRI++E  PS+AKL  LNI  WPKWGCS GKYQLKFEAEETCYLV
Sbjct: 1   MANEEGNSNNPSTNNLRIIIERNPSQAKLSMLNIQSWPKWGCSAGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAY +  GS   +YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+SPS
Sbjct: 61  KGKVKAYAK--GSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of MS002513 vs. ExPASy TrEMBL
Match: A0A6J1DYH1 (uncharacterized protein LOC111024205 OS=Momordica charantia OX=3673 GN=LOC111024205 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.8e-60
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV
Sbjct: 1   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQS S
Sbjct: 61  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSSS 113

[Close]

BLAST of MS002513 vs. ExPASy TrEMBL
Match: A0A6J1KU03 (uncharacterized protein LOC111498188 OS=Cucurbita maxima OX=3661 GN=LOC111498188 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 4.4e-40
Identity = 84/113 (74.34%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           M N       P  N+LRI+VE  PSEAKL  LNI  WPKWGCS GKYQLKFEAEETCYLV
Sbjct: 1   MANEEGNSNNPSTNNLRIIVERNPSEAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAY +  GS   +YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+SPS
Sbjct: 61  KGKVKAYAK--GSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of MS002513 vs. ExPASy TrEMBL
Match: A0A6J1H6X6 (uncharacterized protein LOC111460998 OS=Cucurbita moschata OX=3662 GN=LOC111460998 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.8e-40
Identity = 83/113 (73.45%), Postives = 90/113 (79.65%), Query Frame = 0


Query: 2   MYNRSMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLV 61
           M N       P  N+LRI+VE  PS+AKL  LNI  WPKWGCS GKYQLKFEAEETCYLV
Sbjct: 1   MANEEGNSNNPSTNNLRIIVERNPSQAKLSLLNIQSWPKWGCSAGKYQLKFEAEETCYLV 60

Query: 62  KGKVKAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KGKVKAY +  GS   +YTEFGAGDLVTIPKGLSCTWDVSVAVDKFY+F+SPS
Sbjct: 61  KGKVKAYAK--GSAETEYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYKFESPS 111

[Close]

BLAST of MS002513 vs. ExPASy TrEMBL
Match: A0A2P5AKT6 (RmlC-like cupins superfamily protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_322550 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 9.1e-38
Identity = 76/98 (77.55%), Postives = 86/98 (87.76%), Query Frame = 0


Query: 17  LRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVKAYYQKGGSNC 76
           LRI+VE  PSEAKL ELNI  WPKWGCSPGKYQLKF+AEETCYL+KGKVKAY +  GS+ 
Sbjct: 21  LRIIVEKNPSEAKLSELNIKCWPKWGCSPGKYQLKFDAEETCYLLKGKVKAYPK--GSSS 80

Query: 77  EQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
            ++ EFGAGDLVTIPKGLSCTWDVSVAVDK+Y+F+S S
Sbjct: 81  SEFVEFGAGDLVTIPKGLSCTWDVSVAVDKYYKFESSS 116

[Close]

BLAST of MS002513 vs. ExPASy TrEMBL
Match: A0A2P5C0K2 (RmlC-like cupins superfamily protein OS=Trema orientale OX=63057 GN=TorRG33x02_301930 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.7e-37
Identity = 76/109 (69.72%), Postives = 89/109 (81.65%), Query Frame = 0


Query: 6   SMADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKV 65
           S ++       LRI+VE  PSEA+L ELNI  WPKWGCSPGKYQLKF+AEETCYL+KGKV
Sbjct: 9   SPSNSSSSGTSLRIIVEKNPSEARLSELNIKCWPKWGCSPGKYQLKFDAEETCYLLKGKV 68

Query: 66  KAYYQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
           KAY +  GS+  ++ EFGAGDLVTIPKGLSCTWDVSVAVDK+Y+F+S S
Sbjct: 69  KAYPK--GSSSSEFVEFGAGDLVTIPKGLSCTWDVSVAVDKYYKFESSS 115

[Close]

BLAST of MS002513 vs. TAIR 10
Match: AT4G28703.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 1.1e-32
Identity = 65/111 (58.56%), Postives = 76/111 (68.47%), Query Frame = 0


Query: 7   MADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVK 66
           MADQ P     RI+VE  PS+A+L EL    WPKWGCSPGKY LK+EAEE CY+++GKVK
Sbjct: 1   MADQNP-----RIIVEQNPSQARLDELKFKSWPKWGCSPGKYHLKYEAEEICYILRGKVK 60

Query: 67  AY-----YQKGGSNCEQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQS 113
            Y          +  E   EFGAGD+VT PKGLSCTWDVS++VDK Y F S
Sbjct: 61  VYPKPPPSSSSDAEVEWCVEFGAGDIVTFPKGLSCTWDVSLSVDKHYIFLS 106

[Close]

BLAST of MS002513 vs. TAIR 10
Match: AT3G04300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 5.7e-32
Identity = 60/98 (61.22%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 17  LRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVKAYYQKGGSNC 76
           + IV+E+ PS  +L +L +  WPKW C PGKY L FE  ETCYLVKGKVK  Y KG S  
Sbjct: 1   MNIVIENNPSSRRLSDLGVMSWPKWSCQPGKYALVFEERETCYLVKGKVKV-YPKGSS-- 60

Query: 77  EQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQSPS 115
            ++ EFGAGDLVTIPKGLSCTWDVS+ +DK Y+F  P+
Sbjct: 61  -EFVEFGAGDLVTIPKGLSCTWDVSLFIDKHYKFDPPT 94

[Close]

BLAST of MS002513 vs. TAIR 10
Match: AT4G10300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 7.9e-26
Identity = 49/95 (51.58%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 17  LRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVKAYYQKGGSNC 76
           L I +E  P E+KL +L +  WPKWGC P K+   + A+ETCYL++GKVK Y     +  
Sbjct: 44  LGITIEKNPPESKLTQLGVRSWPKWGCPPSKFPWTYSAKETCYLLQGKVKVY----PNGS 103

Query: 77  EQYTEFGAGDLVTIPKGLSCTWDVSVAVDKFYRFQ 112
           ++  E  AGD V  PKG+SCTWDVSVAVDK Y+F+
Sbjct: 104 DEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQFE 134

[Close]

BLAST of MS002513 vs. TAIR 10
Match: AT2G32650.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 7.0e-06
Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 0


Query: 7   MADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVK 66
           M  ++P      + VE K S+ +L EL +S W  W     K    ++ ++  Y+ +G+V+
Sbjct: 34  MQTEKPLEELYNVKVERKVSQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVR 93

Query: 67  AYYQKGGSNCEQYTEFGAGDLVTIPKGL 95
              +      ++Y +F AGDLV  PK L
Sbjct: 94  VVPE----GSKRYMQFLAGDLVRYPKWL 117

[Close]

BLAST of MS002513 vs. TAIR 10
Match: AT2G32180.1 (plastid transcriptionally active 18 )

HSP 1 Score: 47.8 bits (112), Expect = 7.0e-06
Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 0


Query: 7   MADQEPDNNDLRIVVESKPSEAKLRELNISGWPKWGCSPGKYQLKFEAEETCYLVKGKVK 66
           M  ++P      + VE K S+ +L EL +S W  W     K    ++ ++  Y+ +G+V+
Sbjct: 34  MQTEKPLEELYNVKVERKVSQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVR 93

Query: 67  AYYQKGGSNCEQYTEFGAGDLVTIPKGL 95
              +      ++Y +F AGDLV  PK L
Sbjct: 94  VVPE----GSKRYMQFLAGDLVRYPKWL 117

The following BLAST results are available for this feature:

BLAST of MS002513 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157536.1	7.9e-60	99.12	uncharacterized protein LOC111024205 [Momordica charantia]	[more]
XP_038906875.1	3.1e-40	71.55	uncharacterized protein LOC120092760 [Benincasa hispida]	[more]
XP_023005099.1	9.0e-40	74.34	uncharacterized protein LOC111498188 [Cucurbita maxima] >XP_023005100.1 uncharac...	[more]
KAG7025258.1	1.5e-39	73.45	hypothetical protein SDJN02_11753 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6592849.1	2.0e-39	72.57	hypothetical protein SDJN03_12325, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MS002513 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS002513 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYH1	3.8e-60	99.12	uncharacterized protein LOC111024205 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1KU03	4.4e-40	74.34	uncharacterized protein LOC111498188 OS=Cucurbita maxima OX=3661 GN=LOC111498188...	[more]
A0A6J1H6X6	9.8e-40	73.45	uncharacterized protein LOC111460998 OS=Cucurbita moschata OX=3662 GN=LOC1114609...	[more]
A0A2P5AKT6	9.1e-38	77.55	RmlC-like cupins superfamily protein OS=Parasponia andersonii OX=3476 GN=PanWU01...	[more]
A0A2P5C0K2	2.7e-37	69.72	RmlC-like cupins superfamily protein OS=Trema orientale OX=63057 GN=TorRG33x02_3...	[more]

BLAST of MS002513 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28703.1	1.1e-32	58.56	RmlC-like cupins superfamily protein	[more]
AT3G04300.1	5.7e-32	61.22	RmlC-like cupins superfamily protein	[more]
AT4G10300.1	7.9e-26	51.58	RmlC-like cupins superfamily protein	[more]
AT2G32650.1	7.0e-06	29.55	RmlC-like cupins superfamily protein	[more]
AT2G32180.1	7.0e-06	29.55	plastid transcriptionally active 18	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 8..110 e-value: 1.4E-32 score: 113.3
IPR008579	(S)-ureidoglycine aminohydrolase, cupin-3 domain	PFAM	PF05899	Cupin_3	coord: 32..108 e-value: 7.2E-25 score: 86.5
None	No IPR available	PANTHER	PTHR33271:SF3	OS02G0620400 PROTEIN	coord: 7..113
None	No IPR available	PANTHER	PTHR33271	OS04G0445200 PROTEIN	coord: 7..113
None	No IPR available	CDD	cd02227	cupin_TM1112-like	coord: 38..110 e-value: 6.48372E-26 score: 89.9471
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 33..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002513.1	MS002513.1	mRNA