MS002473 (gene) Bitter gourd (TR) v1

Overview
NameMS002473
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein ROOT INITIATION DEFECTIVE 3-like
Locationscaffold318: 404771 .. 407154 (-)
RNA-Seq ExpressionMS002473
SyntenyMS002473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCTAGTGGTCGCTTCGTCCTCTATTGATTCTGGAATTGGTTGCTGGGACCTCCACACGGGTGCGGAACAACTAAGATACAAGTCTTGTGCTTCTCCTTCTCATGGTCTTATATGTGTTGGTCAACAGTTTCTCGCATGCTCTCAGCTCCGTGATCCCGCTGCCACAGCAGGCTCCATTCTCTACTGGTCTTGGTTTAAGGTACTTGTTATTGCAAGTGTAATAAGATAAATTCTCTTTGCCAATCGTGTGCTTGATTAATTTTCCTCTGTGCAGCCTCAAGTAGAAGTTAAGAGCTTTCCGGCTGAAGCAATAATGCCATTAGCTGCCAACAATGAGGGTACCTATATAGTTGGTGGTGGTTTCTCTGGAGATATTTACTTGTGGGAGGTATAGTTTGATTTATTTTTAAAAAATTTAAAATATTATATACTTTGGTATTGAATTCTAAAATTCATCAACTATGTAAAACTCTACTAGTCTTCCCATTATTTGAGTTCCACATTCTTCTCTGTGTTACTATTTGGCATGCTCATTGCTGCAGGTTTTGAGCGGTAGACTGCTTAAGAAATGGCATGGTCATTATAGGGCAGTTACTTGCGTAGTATTCTCTGAAGACGATTCCCTATTAATTTCCGGTTCTGAAGATGGCTGTGTTAGAGTTTGGTCCCTTCTAACGTAAAAAATTTCATCCTTCACTTTGTTTAGAGTTTATTGTCATTAATTGGAATTATTCTTACGACCGTGTGTATAACAGGGTATTTGATGATGGGTGGCAAAGGGAAGCAAAACATTTATATGAACATAGTTTCACTGGGCACAATCTACCTATTACTGATATTGTTGTAGGTTATGGAGGATCCAACTCTATTATAATCTCTTCCTCCGTTGACCGGACTTGTAAGGTTTGGCACTCTAAAGAATTTCTATGTTCATAATATAATTTTATGAGGATATGGCTGTGTAATTGGCTATTGAAACCGTAGTTGAGGGTGTTTTAGGACGCAGTTAAGTTTCAATTCTAAATATTCAAGGAATGTTTTGAATTGTTTATCTTTTATCACTAACGAGTGATGACTACTTTCCTGCCCATATTTTTGTTATTTATAATTTCGTTTCGGTAAACCATATATGTTTAGTATTATATGTTATATGTTAACTTGGATTTTCTAGGTGTGGAGCTTATCTAAGGGAAAATTGTTAAGAAATATCATATTTCCTTCAATCATTGATGCAATTGCTCTGGATCCTGGCGAACATGTATTCTATGGTGGTGGTAGAGATGGGAAGATATACACTGCTGCGCTCAATGCTAAAAGCCCATCTAGTAGTGATTATGGATTGCACATTCTCGGCTCATTATCAAATCAGAGGTCTGTATCTGTGAAAAGCTTTTTATTGTGTAAACCATTATCTTGTCAAGTTTCATTATTATATCTCACACTTGTCGAGAAGGTTAAGCCGTCCTCAAAATTTTGAGCACTTTCCAATTTTTAGAATATTATTTGTGCTCTGTGCAATTCAAAATTCAATGGGTTTCCTTACTTTAGATAAAACCGAACATGTATAATATGTTTTCCAGTGTGTACAGCAGGATATTATTTGTATTTTTTGTTTTGTCCGGCCTCTAACTTGTTCTACTTGTGTATTTTGAGTAGCAAGTCTGTTACTTCTTTGGCTTACTGTGCAAGTGGAAATTTGTTGATATCTGGATCTGAGGATGGAGCAATTAAAGTTTGGGATACCAGGACCAACAATATCATTCGTGTTCTTAAACATTCAAAAGGTTCCAACTTCCAACTCATCCAAATGTTCATTTCATGCATAAATATCTATTTTGTGGCACTATAAATCAATCCTGTATTAGCTTTTGCTTGATATTGTGCAGGTCCTGTAAATAATATAATTATCGTACGACAACGGGTCATCCCTAAACCGCTGATAAATTCTAGTTCCCAAGGTTCATTGAGAAAGCATCGTTCACTAGTACCTCCTCCCCTTGCAAAATTCGAAAATTCGAAGGACGAGGATGACGACACTGGCATTGTCATTGGTGTATCGGGAGAATTTATGGGGTTTTCTTCCCCCCTTACTTGTGACGTGATGGAGAACCAAATCGCACAACTTCAGGTGCTTGGCCTCCCTCGGGACTCCATTTTTTGTGGCTAACGAAACTTCATCTCTAAAACTTGTATTGACATGCTGTTTTGTTTGTGAACCTACTTCAGAAACAGGGTTGTGCTGCGACAGGAATGGAGTTGGAACGAGTGAAGCGTGACTATGGAAGATCCATGCAAATGCTACAGCAGTGGAGAAAAATGTACGACAATTTACATGAATTTTGTGTAAATGAGCTTGTGGATGGCGATCAAAATACACGG

mRNA sequence

ATGGTGCTAGTGGTCGCTTCGTCCTCTATTGATTCTGGAATTGGTTGCTGGGACCTCCACACGGGTGCGGAACAACTAAGATACAAGTCTTGTGCTTCTCCTTCTCATGGTCTTATATGTGTTGGTCAACAGTTTCTCGCATGCTCTCAGCTCCGTGATCCCGCTGCCACAGCAGGCTCCATTCTCTACTGGTCTTGGTTTAAGCCTCAAGTAGAAGTTAAGAGCTTTCCGGCTGAAGCAATAATGCCATTAGCTGCCAACAATGAGGGTACCTATATAGTTGGTGGTGGTTTCTCTGGAGATATTTACTTGTGGGAGGTTTTGAGCGGTAGACTGCTTAAGAAATGGCATGGTCATTATAGGGCAGTTACTTGCGTAGTATTCTCTGAAGACGATTCCCTATTAATTTCCGGTTCTGAAGATGGCTGTGTTAGAGTTTGGTCCCTTCTAACGGTATTTGATGATGGGTGGCAAAGGGAAGCAAAACATTTATATGAACATAGTTTCACTGGGCACAATCTACCTATTACTGATATTGTTGTAGGTTATGGAGGATCCAACTCTATTATAATCTCTTCCTCCGTTGACCGGACTTGTAAGGTGTGGAGCTTATCTAAGGGAAAATTGTTAAGAAATATCATATTTCCTTCAATCATTGATGCAATTGCTCTGGATCCTGGCGAACATGTATTCTATGGTGGTGGTAGAGATGGGAAGATATACACTGCTGCGCTCAATGCTAAAAGCCCATCTAGTAGTGATTATGGATTGCACATTCTCGGCTCATTATCAAATCAGAGCAAGTCTGTTACTTCTTTGGCTTACTGTGCAAGTGGAAATTTGTTGATATCTGGATCTGAGGATGGAGCAATTAAAGTTTGGGATACCAGGACCAACAATATCATTCGTGTTCTTAAACATTCAAAAGGTCCTGTAAATAATATAATTATCGTACGACAACGGGTCATCCCTAAACCGCTGATAAATTCTAGTTCCCAAGGTTCATTGAGAAAGCATCGTTCACTAGTACCTCCTCCCCTTGCAAAATTCGAAAATTCGAAGGACGAGGATGACGACACTGGCATTGTCATTGGTGTATCGGGAGAATTTATGGGGTTTTCTTCCCCCCTTACTTGTGACGTGATGGAGAACCAAATCGCACAACTTCAGAAACAGGGTTGTGCTGCGACAGGAATGGAGTTGGAACGAGTGAAGCGTGACTATGGAAGATCCATGCAAATGCTACAGCAGTGGAGAAAAATGTACGACAATTTACATGAATTTTGTGTAAATGAGCTTGTGGATGGCGATCAAAATACACGG

Coding sequence (CDS)

ATGGTGCTAGTGGTCGCTTCGTCCTCTATTGATTCTGGAATTGGTTGCTGGGACCTCCACACGGGTGCGGAACAACTAAGATACAAGTCTTGTGCTTCTCCTTCTCATGGTCTTATATGTGTTGGTCAACAGTTTCTCGCATGCTCTCAGCTCCGTGATCCCGCTGCCACAGCAGGCTCCATTCTCTACTGGTCTTGGTTTAAGCCTCAAGTAGAAGTTAAGAGCTTTCCGGCTGAAGCAATAATGCCATTAGCTGCCAACAATGAGGGTACCTATATAGTTGGTGGTGGTTTCTCTGGAGATATTTACTTGTGGGAGGTTTTGAGCGGTAGACTGCTTAAGAAATGGCATGGTCATTATAGGGCAGTTACTTGCGTAGTATTCTCTGAAGACGATTCCCTATTAATTTCCGGTTCTGAAGATGGCTGTGTTAGAGTTTGGTCCCTTCTAACGGTATTTGATGATGGGTGGCAAAGGGAAGCAAAACATTTATATGAACATAGTTTCACTGGGCACAATCTACCTATTACTGATATTGTTGTAGGTTATGGAGGATCCAACTCTATTATAATCTCTTCCTCCGTTGACCGGACTTGTAAGGTGTGGAGCTTATCTAAGGGAAAATTGTTAAGAAATATCATATTTCCTTCAATCATTGATGCAATTGCTCTGGATCCTGGCGAACATGTATTCTATGGTGGTGGTAGAGATGGGAAGATATACACTGCTGCGCTCAATGCTAAAAGCCCATCTAGTAGTGATTATGGATTGCACATTCTCGGCTCATTATCAAATCAGAGCAAGTCTGTTACTTCTTTGGCTTACTGTGCAAGTGGAAATTTGTTGATATCTGGATCTGAGGATGGAGCAATTAAAGTTTGGGATACCAGGACCAACAATATCATTCGTGTTCTTAAACATTCAAAAGGTCCTGTAAATAATATAATTATCGTACGACAACGGGTCATCCCTAAACCGCTGATAAATTCTAGTTCCCAAGGTTCATTGAGAAAGCATCGTTCACTAGTACCTCCTCCCCTTGCAAAATTCGAAAATTCGAAGGACGAGGATGACGACACTGGCATTGTCATTGGTGTATCGGGAGAATTTATGGGGTTTTCTTCCCCCCTTACTTGTGACGTGATGGAGAACCAAATCGCACAACTTCAGAAACAGGGTTGTGCTGCGACAGGAATGGAGTTGGAACGAGTGAAGCGTGACTATGGAAGATCCATGCAAATGCTACAGCAGTGGAGAAAAATGTACGACAATTTACATGAATTTTGTGTAAATGAGCTTGTGGATGGCGATCAAAATACACGG

Protein sequence

MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGSILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKIYTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDTGIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRKMYDNLHEFCVNELVDGDQNTR
Homology
BLAST of MS002473 vs. NCBI nr
Match: XP_022151059.1 (protein ROOT INITIATION DEFECTIVE 3-like [Momordica charantia] >XP_022151066.1 protein ROOT INITIATION DEFECTIVE 3-like [Momordica charantia])

HSP 1 Score: 902.1 bits (2330), Expect = 1.9e-258
Identity = 439/441 (99.55%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS
Sbjct: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY
Sbjct: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV
Sbjct: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSI+DAIALDPGEHVFYGGGRDGKI
Sbjct: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIVDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDED+DT
Sbjct: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDEDT 360

Query: 361 GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK 420
           GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK
Sbjct: 361 GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK 420

Query: 421 MYDNLHEFCVNELVDGDQNTR 442
           MYDNLHEFCVNELVDGDQNTR
Sbjct: 421 MYDNLHEFCVNELVDGDQNTR 441

BLAST of MS002473 vs. NCBI nr
Match: XP_022941447.1 (protein ROOT INITIATION DEFECTIVE 3 [Cucurbita moschata] >KAG6608496.1 Protein ROOT INITIATION DEFECTIVE 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 801.6 bits (2069), Expect = 3.4e-228
Identity = 382/441 (86.62%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSS+DSGIGCWDLHTGAEQLRYKSCASP+HGL+CVG++FLACSQLRDPAAT+GS
Sbjct: 1   MVLVVASSSVDSGIGCWDLHTGAEQLRYKSCASPAHGLVCVGERFLACSQLRDPAATSGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSWFKPQVEVKSFPAE IM LA+NNEGTYIVGGGFSGDIYLWEVLSGRLL KWH HY
Sbjct: 61  VLYWSWFKPQVEVKSFPAEPIMALASNNEGTYIVGGGFSGDIYLWEVLSGRLLMKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLLISGSEDGCVRVWSLL VFDDGWQRE+KHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLISGSEDGCVRVWSLLKVFDDGWQRESKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           +GYGGSN+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 IGYGGSNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNN++IVRQ+++P   +N +SQGSLRKHRSL+PP LAKF NSKD+D+DT
Sbjct: 301 IIRVLKHSKGPVNNLLIVRQQLLPTSQLNPTSQGSLRKHRSLLPPALAKFGNSKDDDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG SG   E MGF SP++  VM+NQI +LQ+QG AATGMELER+KRDYG+SMQM QQ
Sbjct: 361 GVIIGRSGPPMESMGF-SPISSHVMDNQITELQQQGSAATGMELERLKRDYGKSMQMQQQ 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRK+YDNLH++CVNEL+DGDQ
Sbjct: 421 WRKLYDNLHQYCVNELLDGDQ 440

BLAST of MS002473 vs. NCBI nr
Match: XP_023521513.1 (protein ROOT INITIATION DEFECTIVE 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800.0 bits (2065), Expect = 1.0e-227
Identity = 379/441 (85.94%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSS+DSGIGCWDLHTGAEQLRYKSCASP+HGL+CVG++FLACSQLRDPAAT+GS
Sbjct: 1   MVLVVASSSVDSGIGCWDLHTGAEQLRYKSCASPAHGLVCVGERFLACSQLRDPAATSGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSWFKPQVEVKSFPAE IM LA+NNEGTYI+GGGFSGDIYLWEV+SGRLL KWH HY
Sbjct: 61  VLYWSWFKPQVEVKSFPAEPIMALASNNEGTYIIGGGFSGDIYLWEVMSGRLLMKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLLISGSEDGCVRVWSLL VFDDGWQRE+KHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLISGSEDGCVRVWSLLKVFDDGWQRESKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           +GYGGSN+II+SSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 IGYGGSNAIIVSSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNN++IVRQ+++P   +N +SQGSLRKHRSL+PP LAKF NSKD+D+DT
Sbjct: 301 IIRVLKHSKGPVNNLLIVRQQLLPTSQLNPTSQGSLRKHRSLLPPALAKFGNSKDDDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG SG   E MGF SP++  VM+NQI +LQ+QG AATGMELER+KRDYG+SMQM QQ
Sbjct: 361 GVIIGRSGPPVESMGF-SPISSHVMDNQITELQQQGSAATGMELERLKRDYGKSMQMQQQ 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRK+YDNLH++CVNEL+DGDQ
Sbjct: 421 WRKLYDNLHQYCVNELLDGDQ 440

BLAST of MS002473 vs. NCBI nr
Match: XP_022982251.1 (protein ROOT INITIATION DEFECTIVE 3 [Cucurbita maxima])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 376/441 (85.26%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSS+DSGIGCWDLHTGAEQL YKSCASP+HGL+CVG++FLACSQLRDPAAT+GS
Sbjct: 1   MVLVVASSSVDSGIGCWDLHTGAEQLCYKSCASPAHGLVCVGERFLACSQLRDPAATSGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSWFKPQVEVKSFPAE IM LA+NNEGTYIVGGGFSGDIYLWEVLSGRLL KWH HY
Sbjct: 61  VLYWSWFKPQVEVKSFPAEPIMALASNNEGTYIVGGGFSGDIYLWEVLSGRLLMKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+ FSEDDSLLISGSEDGCVRVWSLL VFDDGWQRE+KHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLEFSEDDSLLISGSEDGCVRVWSLLKVFDDGWQRESKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           +GYGGSN+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 IGYGGSNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNN++IV+ +++P   +N +SQGSLRKHRSL+PP LAKF NSKD+D+DT
Sbjct: 301 IIRVLKHSKGPVNNLLIVQHQLLPTSQLNPTSQGSLRKHRSLLPPALAKFGNSKDDDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG SG   E MGF SP++  VM++QI +LQ+QG AATGMELER+KR+YG+SMQM QQ
Sbjct: 361 GVIIGRSGPPMESMGF-SPISSHVMDHQITELQQQGSAATGMELERLKREYGKSMQMQQQ 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRK+YDNLH++CVNEL+DGDQ
Sbjct: 421 WRKLYDNLHQYCVNELLDGDQ 440

BLAST of MS002473 vs. NCBI nr
Match: XP_038898957.1 (protein ROOT INITIATION DEFECTIVE 3 [Benincasa hispida])

HSP 1 Score: 778.5 bits (2009), Expect = 3.1e-221
Identity = 374/441 (84.81%), Postives = 411/441 (93.20%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASP+HGL+CVG++FLACSQLRDPAATAGS
Sbjct: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPAHGLVCVGERFLACSQLRDPAATAGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSW KPQVEVKSFPAE IM LA+N+EGTYIVGGG SGDIYLWEVLSGRLLKKWH HY
Sbjct: 61  VLYWSWCKPQVEVKSFPAEPIMALASNDEGTYIVGGGLSGDIYLWEVLSGRLLKKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLLISGSEDGC+RVW+LLTVFDDGWQREAKHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLISGSEDGCIRVWALLTVFDDGWQREAKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           VGYGGSN+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIID IALDPGEHVFYGGGRDGKI
Sbjct: 181 VGYGGSNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDVIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAK PS+ DYGLHILGSLSNQSKSVT+LAYC+SGNLLISGSEDGAI+VWDTRTNN
Sbjct: 241 YTAALNAKCPSTGDYGLHILGSLSNQSKSVTALAYCSSGNLLISGSEDGAIRVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           +IRV +HSKGPVNNI++VRQ+V+PK    S+SQG LRKHRSL+PP LAKFENSKDED+DT
Sbjct: 301 VIRVFRHSKGPVNNILLVRQQVLPKS--QSTSQGFLRKHRSLLPPALAKFENSKDEDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG +G   E MGF + L+  VM+NQI +LQ+QG AA GMELER+K D G+SMQMLQ 
Sbjct: 361 GVIIGPTGLPKESMGFPA-LSSHVMDNQITELQQQGSAANGMELERLKYDCGKSMQMLQH 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRKMYDNLH+FCVNEL+DGDQ
Sbjct: 421 WRKMYDNLHQFCVNELLDGDQ 438

BLAST of MS002473 vs. ExPASy Swiss-Prot
Match: Q9M3B4 (Protein ROOT INITIATION DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=RID3 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.0e-142
Identity = 254/445 (57.08%), Postives = 327/445 (73.48%), Query Frame = 0

Query: 2   VLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGSI 61
           + V+ASSSID GIG WDL TG EQL++K CASP+HGL  VG++FLA SQL     T+GSI
Sbjct: 3   ITVIASSSIDEGIGSWDLKTGTEQLQFKPCASPAHGLTAVGEKFLASSQL-SARNTSGSI 62

Query: 62  LYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYR 121
            YWSW KPQ EVKS+P E I  LAANNEGTY+VGGG SGDIYLWEV +G+LLKKWHGHYR
Sbjct: 63  FYWSWTKPQAEVKSYPVEPIKALAANNEGTYLVGGGISGDIYLWEVATGKLLKKWHGHYR 122

Query: 122 AVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVV 181
           +VTC+VFS DDSLL+SGS+DG +RVWSL+ +FDD  +++   LYEH+F  H + +TDIV+
Sbjct: 123 SVTCLVFSGDDSLLVSGSQDGSIRVWSLIRLFDDFQRQQGNTLYEHNFNEHTMSVTDIVI 182

Query: 182 GYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKIY 241
            YGG N++IISSS DRTCKVWSLS+GKLL+NIIFPS+I+A+ALDPG  VFY G RD KIY
Sbjct: 183 DYGGCNAVIISSSEDRTCKVWSLSRGKLLKNIIFPSVINALALDPGGCVFYAGARDSKIY 242

Query: 242 TAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNI 301
             A+NA    +S+YG  +LGS+S + K++T LAYCA GNLLISGSEDG + VWD ++   
Sbjct: 243 IGAINA----TSEYGTQVLGSVSEKGKAITCLAYCADGNLLISGSEDGVVCVWDPKSLRH 302

Query: 302 IRVLKHS----KGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDED 361
           +R L H+    KGPVNNI IVR+ ++     ++ +Q S +   +L+PPPL K+E    ED
Sbjct: 303 VRTLIHAKGSRKGPVNNIQIVRKTIVAN---SNKTQVSWKSRGALIPPPLEKYERPV-ED 362

Query: 362 DDTGIVIGVSGEFMG---FSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQM 421
              GIV      F     +SS L+ D+++ Q+ +LQ+QG AAT ME+ER+K +Y RS+QM
Sbjct: 363 TMDGIVTVDPPPFSDVPVYSSFLSADLIDEQVKELQQQGSAATEMEMERLKLEYKRSLQM 422

Query: 422 LQQWRKMYDNLHEFCVNELVDGDQN 440
            +QW+K Y+NL +  + E   G  N
Sbjct: 423 NEQWQKNYENLLQVVMEEEQIGGTN 438

BLAST of MS002473 vs. ExPASy Swiss-Prot
Match: A0A1L8HX76 (WD repeat-containing protein 18 OS=Xenopus laevis OX=8355 GN=wdr18 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.0e-41
Identity = 109/338 (32.25%), Postives = 174/338 (51.48%), Query Frame = 0

Query: 3   LVVASSSIDSGIGC--WDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 62
           LV+++ +      C  W+  TG+  L Y+   +   GL  +G Q+L   QL         
Sbjct: 7   LVLSADAAGQVANCTVWEPVTGSVLLTYRGGNTSHRGLAVLGGQYLLGGQL-----GKNY 66

Query: 63  ILYWS-WFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGH 122
           I  W    + Q++ K      +  L+A+  G Y+V  G +  IYLWEV +G LL   + H
Sbjct: 67  INVWELQRRDQLQQKIVCPGPVNCLSASPNGLYLV-AGIAESIYLWEVSTGHLLAILNSH 126

Query: 123 YRAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDI 182
           Y+ VTC+ F++D S +ISG++D  V VW L +V      R  +  Y   ++ H+LPITD+
Sbjct: 127 YQDVTCLTFTDDSSHIISGAKDSLVLVWGLYSVLQVETSRGPEPRY--VWSRHSLPITDV 186

Query: 183 VVGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGK 242
             G GG  + + +SS+D+T K+W +  G LL +++F   I ++A DP E+  + GG DG 
Sbjct: 187 QCGIGGPQARVATSSLDQTIKLWDICSGDLLVSVLFDVRITSVAFDPAEYHLFCGGSDGV 246

Query: 243 IYTAALNAKSPSSSDYGLHILGSL--SNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTR 302
           IY   L   +P   +   H    +        VT L+    G++LISGS D  + VWD +
Sbjct: 247 IYQVDLYT-TPEQRERPFHPEQEMVFKGHRNQVTCLSVSLDGSMLISGSHDETVCVWDIQ 306

Query: 303 TNNIIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGS 336
           +   +R + H +GPV N+ I+     P  ++ + S+ S
Sbjct: 307 SKQSLRSVPH-RGPVTNVFII---AAPSNMLRAESKPS 331

BLAST of MS002473 vs. ExPASy Swiss-Prot
Match: Q9BV38 (WD repeat-containing protein 18 OS=Homo sapiens OX=9606 GN=WDR18 PE=1 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 1.6e-39
Identity = 105/326 (32.21%), Postives = 168/326 (51.53%), Query Frame = 0

Query: 17  WDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGSILYWSWFKPQVEVKSF 76
           W+LH+GA  L Y+   +   GL  +  ++L  +QL     +A  +      K Q++ K  
Sbjct: 23  WELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQR----KDQLQQKIM 82

Query: 77  PAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDSLLI 136
               +  L A+  G Y++  G +  I+LWEV +G LL     HY+ V+C+ F+ D S  I
Sbjct: 83  CPGPVTCLTASPNGLYVL-AGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFI 142

Query: 137 SGSEDGCVRVWSLLTVFDDGWQR--EAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSS 196
           SG +D  V VWSL +V      R    +H++ H    H LPITD+  G+GG  + + +SS
Sbjct: 143 SGGKDCLVLVWSLCSVLQADPSRIPAPRHVWSH----HALPITDLHCGFGGPLARVATSS 202

Query: 197 VDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKIYTAALNAKSPSSSD 256
           +D+T K+W +S G+LL +++F   I A+ +D  EH  + GG +G I+   L    P   +
Sbjct: 203 LDQTVKLWEVSSGELLLSVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDL-FTWPGQRE 262

Query: 257 YGLHILGSLSNQSK----SVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLKHSKG 316
              H         K     VT L+    G++L+SGS D  +++WD ++   IR +   KG
Sbjct: 263 RSFHPEQDAGKVFKGHRNQVTCLSVSTDGSVLLSGSHDETVRLWDVQSKQCIRTVA-LKG 322

Query: 317 PVNNIIIVRQRVIPKPLINSSSQGSL 337
           PV N  I+   + P  +++S  + SL
Sbjct: 323 PVTNAAIL---LAPVSMLSSDFRPSL 334

BLAST of MS002473 vs. ExPASy Swiss-Prot
Match: Q3SZD4 (WD repeat-containing protein 18 OS=Bos taurus OX=9913 GN=WDR18 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.8e-38
Identity = 101/311 (32.48%), Postives = 157/311 (50.48%), Query Frame = 0

Query: 17  WDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGSILYWSW---FKPQVEV 76
           W+LH+GA  L Y+   +   GL  +  ++L  +QL       G     +W    K Q++ 
Sbjct: 23  WELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQL-------GKNYICAWELQRKDQLQQ 82

Query: 77  KSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDS 136
           K      +  L  +  G Y++  G S +IYLWEV +G LL     HY+ V+C+ F+ D S
Sbjct: 83  KIMCPGPVTCLTTSPNGLYVL-AGISENIYLWEVSTGNLLVILSRHYQDVSCLQFTGDSS 142

Query: 137 LLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISS 196
             ISG +D  V  WSL +V      R       H ++ H LPITD+  G+GG  + + ++
Sbjct: 143 HFISGGKDCLVLAWSLCSVLQADPSRTPAP--RHVWSRHTLPITDLHCGFGGPLARVATA 202

Query: 197 SVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKIYTAAL------NA 256
           S+D+T K+W +S G+LL +++F   I A+ +D  EH  + GG DG I+   L        
Sbjct: 203 SLDQTVKLWEVSSGELLLSVLFDVGILAVTMDLAEHYMFCGGSDGSIFQVDLCTWPGQRE 262

Query: 257 KSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLKH 316
           KS         +     NQ   VT L+    G++L+SGS D  +++WD ++   +R +  
Sbjct: 263 KSFQPEQEHGKVFRGHRNQ---VTCLSVSTDGSVLLSGSHDETVRLWDVQSQQCLRTVT- 319

Query: 317 SKGPVNNIIIV 319
            KGPV N  I+
Sbjct: 323 LKGPVTNACIM 319

BLAST of MS002473 vs. ExPASy Swiss-Prot
Match: Q68EI0 (WD repeat-containing protein 18 OS=Danio rerio OX=7955 GN=wdr18 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.5e-37
Identity = 123/446 (27.58%), Postives = 208/446 (46.64%), Query Frame = 0

Query: 3   LVVASSSIDSGIGC--WDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 62
           +V+++ S      C  +D HTG+E L Y+   + S  L  +  +++  +QL         
Sbjct: 7   VVLSADSAGQLFNCAVYDPHTGSEFLSYRGGNTSSRSLTILNGEYILGAQLGKNYINVWE 66

Query: 63  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 122
           I      K Q++ K      +  L A+ +G Y++  G +  IYLWEV +G LL     H+
Sbjct: 67  IQR----KDQLQQKIVCPGIVTCLCASPDGLYVL-AGIAEAIYLWEVSTGNLLAILSRHF 126

Query: 123 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTV--FDDGWQREAKHLYEHSFTGHNLPITD 182
           + ++C+ F++D S  +SG +D    +W+L +V   D     E +H+     + H+LPITD
Sbjct: 127 QDLSCIKFTDDSSHFVSGGKDNLAFIWNLSSVVQLDSSRTPEPRHI----LSRHSLPITD 186

Query: 183 IVVGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDG 242
           I  G  G  + + ++S+D+T KVW +S G++L +++F   I ++  DP E+  + GG DG
Sbjct: 187 IHCGLMGPQARVATASLDQTVKVWEISSGEMLLSVLFDVGIMSVTFDPCEYFLFCGGSDG 246

Query: 243 KIYTAALNAKSPS------SSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIK 302
            I+  +L + S S      S   G  +     N    VT L+    G +L+SGS D  ++
Sbjct: 247 NIFQVSLCSTSLSRDKTFQSDSEGNQVFKGHRN---LVTCLSVSMDGTVLLSGSNDETVR 306

Query: 303 VWDTRTNNIIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFEN 362
           +WD ++   I  + H +GPV N  I     IP P     S      H ++  P  ++  N
Sbjct: 307 MWDVQSKQCIWTINH-RGPVTNAAI-----IPAPANMFLSD----SHPAVPLPRFSRHLN 366

Query: 363 SKDEDDDTGIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQG------CAATGMEL----E 422
             ++ D TG          G S  L  +  E +   L+K        CA T   +    E
Sbjct: 367 PSEQGDGTGT--------GGMSLRLGANTQEPEGTYLEKAEELYSLMCAVTDKSVFGDGE 422

Query: 423 RVKRDYGRSMQMLQQWRKMYDNLHEF 429
             K       + ++  +K+  +L+EF
Sbjct: 427 NTKVRVSELEEEVRTLKKINKDLYEF 422

BLAST of MS002473 vs. ExPASy TrEMBL
Match: A0A6J1DDG0 (protein ROOT INITIATION DEFECTIVE 3-like OS=Momordica charantia OX=3673 GN=LOC111019080 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.0e-259
Identity = 439/441 (99.55%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS
Sbjct: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY
Sbjct: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV
Sbjct: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSI+DAIALDPGEHVFYGGGRDGKI
Sbjct: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIVDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDED+DT
Sbjct: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDEDT 360

Query: 361 GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK 420
           GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK
Sbjct: 361 GIVIGVSGEFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQWRK 420

Query: 421 MYDNLHEFCVNELVDGDQNTR 442
           MYDNLHEFCVNELVDGDQNTR
Sbjct: 421 MYDNLHEFCVNELVDGDQNTR 441

BLAST of MS002473 vs. ExPASy TrEMBL
Match: A0A6J1FMG7 (protein ROOT INITIATION DEFECTIVE 3 OS=Cucurbita moschata OX=3662 GN=LOC111446741 PE=4 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.7e-228
Identity = 382/441 (86.62%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSS+DSGIGCWDLHTGAEQLRYKSCASP+HGL+CVG++FLACSQLRDPAAT+GS
Sbjct: 1   MVLVVASSSVDSGIGCWDLHTGAEQLRYKSCASPAHGLVCVGERFLACSQLRDPAATSGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSWFKPQVEVKSFPAE IM LA+NNEGTYIVGGGFSGDIYLWEVLSGRLL KWH HY
Sbjct: 61  VLYWSWFKPQVEVKSFPAEPIMALASNNEGTYIVGGGFSGDIYLWEVLSGRLLMKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLLISGSEDGCVRVWSLL VFDDGWQRE+KHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLISGSEDGCVRVWSLLKVFDDGWQRESKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           +GYGGSN+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 IGYGGSNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNN++IVRQ+++P   +N +SQGSLRKHRSL+PP LAKF NSKD+D+DT
Sbjct: 301 IIRVLKHSKGPVNNLLIVRQQLLPTSQLNPTSQGSLRKHRSLLPPALAKFGNSKDDDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG SG   E MGF SP++  VM+NQI +LQ+QG AATGMELER+KRDYG+SMQM QQ
Sbjct: 361 GVIIGRSGPPMESMGF-SPISSHVMDNQITELQQQGSAATGMELERLKRDYGKSMQMQQQ 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRK+YDNLH++CVNEL+DGDQ
Sbjct: 421 WRKLYDNLHQYCVNELLDGDQ 440

BLAST of MS002473 vs. ExPASy TrEMBL
Match: A0A6J1IYT8 (protein ROOT INITIATION DEFECTIVE 3 OS=Cucurbita maxima OX=3661 GN=LOC111481134 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.5e-225
Identity = 376/441 (85.26%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVLVVASSS+DSGIGCWDLHTGAEQL YKSCASP+HGL+CVG++FLACSQLRDPAAT+GS
Sbjct: 1   MVLVVASSSVDSGIGCWDLHTGAEQLCYKSCASPAHGLVCVGERFLACSQLRDPAATSGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSWFKPQVEVKSFPAE IM LA+NNEGTYIVGGGFSGDIYLWEVLSGRLL KWH HY
Sbjct: 61  VLYWSWFKPQVEVKSFPAEPIMALASNNEGTYIVGGGFSGDIYLWEVLSGRLLMKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+ FSEDDSLLISGSEDGCVRVWSLL VFDDGWQRE+KHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLEFSEDDSLLISGSEDGCVRVWSLLKVFDDGWQRESKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           +GYGGSN+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 IGYGGSNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISGSEDGAIKVWDTRTNN
Sbjct: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGSEDGAIKVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           IIRVLKHSKGPVNN++IV+ +++P   +N +SQGSLRKHRSL+PP LAKF NSKD+D+DT
Sbjct: 301 IIRVLKHSKGPVNNLLIVQHQLLPTSQLNPTSQGSLRKHRSLLPPALAKFGNSKDDDEDT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++IG SG   E MGF SP++  VM++QI +LQ+QG AATGMELER+KR+YG+SMQM QQ
Sbjct: 361 GVIIGRSGPPMESMGF-SPISSHVMDHQITELQQQGSAATGMELERLKREYGKSMQMQQQ 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRK+YDNLH++CVNEL+DGDQ
Sbjct: 421 WRKLYDNLHQYCVNELLDGDQ 440

BLAST of MS002473 vs. ExPASy TrEMBL
Match: A0A0A0KZT0 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639920 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.0e-219
Identity = 370/441 (83.90%), Postives = 409/441 (92.74%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVL+VASSSIDSGIGCWDLHTG+EQLRYKSCASP HGL+CVG++FLACSQLRDPAATAGS
Sbjct: 1   MVLLVASSSIDSGIGCWDLHTGSEQLRYKSCASPPHGLVCVGRKFLACSQLRDPAATAGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSW KPQVEVKSFPAE IM LA+N+EGTYIVGGGFSGDIYLWEVLSGRLLKKWH HY
Sbjct: 61  VLYWSWSKPQVEVKSFPAEPIMALASNHEGTYIVGGGFSGDIYLWEVLSGRLLKKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLL+SGSEDGC+RVWSL+TVFDDGWQREAKHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLVSGSEDGCIRVWSLITVFDDGWQREAKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           VGYGG N+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 VGYGGFNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAK PSSSDYGLHILGS+SNQSKSVTSLAYC+SGNLLISGSEDGAI+VWDTRTNN
Sbjct: 241 YTAALNAKCPSSSDYGLHILGSISNQSKSVTSLAYCSSGNLLISGSEDGAIRVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           +IRV +HSKGPVNNI++V+Q+ +PK    S+SQGSLRKHR L+PP LAKFENSKDED+ T
Sbjct: 301 VIRVFRHSKGPVNNILLVQQQPLPKS--QSTSQGSLRKHRPLLPPALAKFENSKDEDEYT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++I  SG   E  GFS  L+  VM++QI +LQ+QG AATGMELER+K DYG+S QMLQ 
Sbjct: 361 GVIIDPSGPPKESTGFSF-LSSHVMDDQITELQQQGSAATGMELERLKHDYGKSKQMLQH 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRKMYDNLH+FCVNEL+DG+Q
Sbjct: 421 WRKMYDNLHQFCVNELLDGNQ 438

BLAST of MS002473 vs. ExPASy TrEMBL
Match: A0A1S3BU36 (protein ROOT INITIATION DEFECTIVE 3 OS=Cucumis melo OX=3656 GN=LOC103493552 PE=4 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 7.7e-218
Identity = 371/441 (84.13%), Postives = 406/441 (92.06%), Query Frame = 0

Query: 1   MVLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGS 60
           MVL+VASSSIDSGIGCWDLHTGAEQLRYKSCASP HGL+CVG++FLACSQLRD AATAGS
Sbjct: 1   MVLLVASSSIDSGIGCWDLHTGAEQLRYKSCASPPHGLVCVGEKFLACSQLRDAAATAGS 60

Query: 61  ILYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHY 120
           +LYWSW KPQVEVKSFPAE IM LA+NNEGTYIVGGGFSGDIYLWEVLSGRLLKKWH HY
Sbjct: 61  VLYWSWCKPQVEVKSFPAEPIMALASNNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHAHY 120

Query: 121 RAVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIV 180
           RAVTC+VFSEDDSLLISGSEDGC+RVWSL+TVFDDGWQREAKHLYEHSFTGHNLP+TDIV
Sbjct: 121 RAVTCLVFSEDDSLLISGSEDGCIRVWSLITVFDDGWQREAKHLYEHSFTGHNLPVTDIV 180

Query: 181 VGYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240
           VGYGG N+IIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI
Sbjct: 181 VGYGGFNAIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKI 240

Query: 241 YTAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNN 300
           YTAALNAK PSSSDYGLHILGSLSNQSKSVTSLAYC+SGNLLISG EDGAI+VWDTRTNN
Sbjct: 241 YTAALNAKCPSSSDYGLHILGSLSNQSKSVTSLAYCSSGNLLISGYEDGAIRVWDTRTNN 300

Query: 301 IIRVLKHSKGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDEDDDT 360
           +IRV +HSKGPVNNI++V+Q+ +PK    S+SQGSLRKHR ++PP LAKFENSKDED+ T
Sbjct: 301 VIRVFRHSKGPVNNILLVQQQPLPKS--QSTSQGSLRKHRPILPPALAKFENSKDEDEYT 360

Query: 361 GIVIGVSG---EFMGFSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQMLQQ 420
           G++I  SG   E M FS  L+  VM+NQI +LQ+QG AAT MELER+K DYG+S QMLQ 
Sbjct: 361 GVIIDPSGPPKESMEFSF-LSSHVMDNQITELQQQGSAATEMELERLKHDYGKSKQMLQH 420

Query: 421 WRKMYDNLHEFCVNELVDGDQ 439
           WRKMYDNLH+FCVNEL+DG+Q
Sbjct: 421 WRKMYDNLHQFCVNELLDGNQ 438

BLAST of MS002473 vs. TAIR 10
Match: AT3G49180.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 508.1 bits (1307), Expect = 7.3e-144
Identity = 254/445 (57.08%), Postives = 327/445 (73.48%), Query Frame = 0

Query: 2   VLVVASSSIDSGIGCWDLHTGAEQLRYKSCASPSHGLICVGQQFLACSQLRDPAATAGSI 61
           + V+ASSSID GIG WDL TG EQL++K CASP+HGL  VG++FLA SQL     T+GSI
Sbjct: 3   ITVIASSSIDEGIGSWDLKTGTEQLQFKPCASPAHGLTAVGEKFLASSQL-SARNTSGSI 62

Query: 62  LYWSWFKPQVEVKSFPAEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYR 121
            YWSW KPQ EVKS+P E I  LAANNEGTY+VGGG SGDIYLWEV +G+LLKKWHGHYR
Sbjct: 63  FYWSWTKPQAEVKSYPVEPIKALAANNEGTYLVGGGISGDIYLWEVATGKLLKKWHGHYR 122

Query: 122 AVTCVVFSEDDSLLISGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVV 181
           +VTC+VFS DDSLL+SGS+DG +RVWSL+ +FDD  +++   LYEH+F  H + +TDIV+
Sbjct: 123 SVTCLVFSGDDSLLVSGSQDGSIRVWSLIRLFDDFQRQQGNTLYEHNFNEHTMSVTDIVI 182

Query: 182 GYGGSNSIIISSSVDRTCKVWSLSKGKLLRNIIFPSIIDAIALDPGEHVFYGGGRDGKIY 241
            YGG N++IISSS DRTCKVWSLS+GKLL+NIIFPS+I+A+ALDPG  VFY G RD KIY
Sbjct: 183 DYGGCNAVIISSSEDRTCKVWSLSRGKLLKNIIFPSVINALALDPGGCVFYAGARDSKIY 242

Query: 242 TAALNAKSPSSSDYGLHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNI 301
             A+NA    +S+YG  +LGS+S + K++T LAYCA GNLLISGSEDG + VWD ++   
Sbjct: 243 IGAINA----TSEYGTQVLGSVSEKGKAITCLAYCADGNLLISGSEDGVVCVWDPKSLRH 302

Query: 302 IRVLKHS----KGPVNNIIIVRQRVIPKPLINSSSQGSLRKHRSLVPPPLAKFENSKDED 361
           +R L H+    KGPVNNI IVR+ ++     ++ +Q S +   +L+PPPL K+E    ED
Sbjct: 303 VRTLIHAKGSRKGPVNNIQIVRKTIVAN---SNKTQVSWKSRGALIPPPLEKYERPV-ED 362

Query: 362 DDTGIVIGVSGEFMG---FSSPLTCDVMENQIAQLQKQGCAATGMELERVKRDYGRSMQM 421
              GIV      F     +SS L+ D+++ Q+ +LQ+QG AAT ME+ER+K +Y RS+QM
Sbjct: 363 TMDGIVTVDPPPFSDVPVYSSFLSADLIDEQVKELQQQGSAATEMEMERLKLEYKRSLQM 422

Query: 422 LQQWRKMYDNLHEFCVNELVDGDQN 440
            +QW+K Y+NL +  + E   G  N
Sbjct: 423 NEQWQKNYENLLQVVMEEEQIGGTN 438

BLAST of MS002473 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 7.6e-16
Identity = 64/260 (24.62%), Postives = 111/260 (42.69%), Query Frame = 0

Query: 81  IMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDSLLISGSE 140
           I  +A +++  +IV       + LW+V +G L+K   GH     CV F+   ++++SGS 
Sbjct: 74  ISDVAFSSDARFIVSASDDKTLKLWDVETGSLIKTLIGHTNYAFCVNFNPQSNMIVSGSF 133

Query: 141 DGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSSVDRTCK 200
           D  VR+W + T          K L       H+ P+T   V +    S+I+SSS D  C+
Sbjct: 134 DETVRIWDVTT---------GKCL--KVLPAHSDPVT--AVDFNRDGSLIVSSSYDGLCR 193

Query: 201 VWSLSKGKLLRNIIFPS--IIDAIALDPGEHVFYGGGRDGKIYTAALNAKSPSSSDYGLH 260
           +W    G  ++ +I      +  +   P       G  D       L   + SS+ +   
Sbjct: 194 IWDSGTGHCVKTLIDDENPPVSFVRFSPNGKFILVGTLDN-----TLRLWNISSAKFLKT 253

Query: 261 ILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLKHSKGPVNNIIIV 320
             G + N    ++S     +G  ++SGSED  + +W+  +  +++ L+     V N+   
Sbjct: 254 YTGHV-NAQYCISSAFSVTNGKRIVSGSEDNCVHMWELNSKKLLQKLEGHTETVMNVAC- 307

Query: 321 RQRVIPKPLINSSSQGSLRK 339
                  P  N  + GSL K
Sbjct: 314 ------HPTENLIASGSLDK 307

BLAST of MS002473 vs. TAIR 10
Match: AT3G18130.1 (receptor for activated C kinase 1C )

HSP 1 Score: 82.4 bits (202), Expect = 9.9e-16
Identity = 55/221 (24.89%), Postives = 109/221 (49.32%), Query Frame = 0

Query: 87  NNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDSLLISGSEDGCVRV 146
           +++G + + G + G++ LW++ +G   +++ GH + V  V FS D+  ++S S D  +++
Sbjct: 72  SSDGQFALSGSWDGELRLWDLATGETTRRFVGHTKDVLSVAFSTDNRQIVSASRDRTIKL 131

Query: 147 WSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSSVDRTCKVWSLSK 206
           W+ L         E K+       GH   ++ +          I+S+S D+T KVW+L  
Sbjct: 132 WNTL--------GECKYTISEG-DGHKEWVSCVRFSPNTLVPTIVSASWDKTVKVWNLQN 191

Query: 207 GKLLRNIIFPS-IIDAIALDPGEHVFYGGGRDGKIYTAALNAKSPSSSDYGLHILGSLSN 266
            KL  +++  S  ++ +A+ P   +   GG+DG I    L   +     Y L   GS+  
Sbjct: 192 CKLRNSLVGHSGYLNTVAVSPDGSLCASGGKDGVILLWDL---AEGKKLYSLE-AGSI-- 251

Query: 267 QSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLK 307
               + SL +  +   L + +E+ +I++WD  + +++  LK
Sbjct: 252 ----IHSLCFSPNRYWLCAATEN-SIRIWDLESKSVVEDLK 272

BLAST of MS002473 vs. TAIR 10
Match: AT1G48630.1 (receptor for activated C kinase 1B )

HSP 1 Score: 79.3 bits (194), Expect = 8.4e-15
Identity = 66/265 (24.91%), Postives = 111/265 (41.89%), Query Frame = 0

Query: 81  IMPLAANNEGTYIVGGGFSGDIYLWEVLSGRLLKKWHGHYRAVTCVVFSEDDSLLISGSE 140
           +  +  +++G + + G + G++ LW++ +G   +++ GH + V  V FS D+  ++S S 
Sbjct: 66  VQDVVLSSDGQFALSGSWDGELRLWDLATGESTRRFVGHTKDVLSVAFSTDNRQIVSASR 125

Query: 141 DGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSSVDRTCK 200
           D  +++W+ L         E K+    +  GH   ++ +          I+S+S D+T K
Sbjct: 126 DRTIKLWNTL--------GECKYTISEA-DGHKEWVSCVRFSPNTLVPTIVSASWDKTVK 185

Query: 201 VWSLSKGKLLRNIIFPS-IIDAIALDPGEHVFYGGGRDGKIYTAAL-NAKSPSSSDYG-- 260
           VW+L   KL   +   S  ++ +A+ P   +   GG+DG I    L   K   S + G  
Sbjct: 186 VWNLQNCKLRNTLAGHSGYLNTVAVSPDGSLCASGGKDGVILLWDLAEGKKLYSLEAGSI 245

Query: 261 LHILGSLSN-----------------QSKSV----------------------------- 295
           +H L    N                 +SKSV                             
Sbjct: 246 IHSLCFSPNRYWLCAATENSIRIWDLESKSVVEDLKVDLKAEAEKTDGSTGIGNKTKVIY 305

BLAST of MS002473 vs. TAIR 10
Match: AT4G02730.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 8.4e-15
Identity = 59/237 (24.89%), Postives = 103/237 (43.46%), Query Frame = 0

Query: 78  AEAIMPLAANNEGTYIVGGGFSGDIYLWEVLSG-RLLKKWHGHYRAVTCVVFSEDDSLLI 137
           +  I  LA +++  Y         + +W+  S    LK   GH   V CV F+   +L++
Sbjct: 85  SSGISDLAWSSDSHYTCSASDDCTLRIWDARSPYECLKVLRGHTNFVFCVNFNPPSNLIV 144

Query: 138 SGSEDGCVRVWSLLTVFDDGWQREAKHLYEHSFTGHNLPITDIVVGYGGSNSIIISSSVD 197
           SGS D  +R+W + T                    H++PI+   V +    S+I+S+S D
Sbjct: 145 SGSFDETIRIWEVKT-----------GKCVRMIKAHSMPISS--VHFNRDGSLIVSASHD 204

Query: 198 RTCKVWSLSKGKLLRNII---FPSIIDAIALDPGEHVFYGGGRDGKIYTAALNAKSPSSS 257
            +CK+W   +G  L+ +I    P++            F     +GK    A    +   S
Sbjct: 205 GSCKIWDAKEGTCLKTLIDDKSPAV-----------SFAKFSPNGKFILVATLDSTLKLS 264

Query: 258 DYG----LHILGSLSNQSKSVTSLAYCASGNLLISGSEDGAIKVWDTRTNNIIRVLK 307
           +Y     L +    +N+   +TS     +G  ++SGSED  + +WD +  NI++ L+
Sbjct: 265 NYATGKFLKVYTGHTNKVFCITSAFSVTNGKYIVSGSEDNCVYLWDLQARNILQRLE 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151059.11.9e-25899.55protein ROOT INITIATION DEFECTIVE 3-like [Momordica charantia] >XP_022151066.1 p... [more]
XP_022941447.13.4e-22886.62protein ROOT INITIATION DEFECTIVE 3 [Cucurbita moschata] >KAG6608496.1 Protein R... [more]
XP_023521513.11.0e-22785.94protein ROOT INITIATION DEFECTIVE 3 [Cucurbita pepo subsp. pepo][more]
XP_022982251.11.8e-22485.26protein ROOT INITIATION DEFECTIVE 3 [Cucurbita maxima][more]
XP_038898957.13.1e-22184.81protein ROOT INITIATION DEFECTIVE 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M3B41.0e-14257.08Protein ROOT INITIATION DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=RID3 PE=1... [more]
A0A1L8HX763.0e-4132.25WD repeat-containing protein 18 OS=Xenopus laevis OX=8355 GN=wdr18 PE=3 SV=1[more]
Q9BV381.6e-3932.21WD repeat-containing protein 18 OS=Homo sapiens OX=9606 GN=WDR18 PE=1 SV=2[more]
Q3SZD41.8e-3832.48WD repeat-containing protein 18 OS=Bos taurus OX=9913 GN=WDR18 PE=2 SV=1[more]
Q68EI01.5e-3727.58WD repeat-containing protein 18 OS=Danio rerio OX=7955 GN=wdr18 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DDG09.0e-25999.55protein ROOT INITIATION DEFECTIVE 3-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1FMG71.7e-22886.62protein ROOT INITIATION DEFECTIVE 3 OS=Cucurbita moschata OX=3662 GN=LOC11144674... [more]
A0A6J1IYT88.5e-22585.26protein ROOT INITIATION DEFECTIVE 3 OS=Cucurbita maxima OX=3661 GN=LOC111481134 ... [more]
A0A0A0KZT07.0e-21983.90WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A1S3BU367.7e-21884.13protein ROOT INITIATION DEFECTIVE 3 OS=Cucumis melo OX=3656 GN=LOC103493552 PE=4... [more]
Match NameE-valueIdentityDescription
AT3G49180.17.3e-14457.08Transducin/WD40 repeat-like superfamily protein [more]
AT3G49660.17.6e-1624.62Transducin/WD40 repeat-like superfamily protein [more]
AT3G18130.19.9e-1624.89receptor for activated C kinase 1C [more]
AT1G48630.18.4e-1524.91receptor for activated C kinase 1B [more]
AT4G02730.18.4e-1524.89Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020472G-protein beta WD-40 repeatPRINTSPR00320GPROTEINBRPTcoord: 135..149
score: 39.79
coord: 282..296
score: 40.29
coord: 190..204
score: 36.27
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 206..244
e-value: 350.0
score: 1.0
coord: 256..295
e-value: 7.7E-7
score: 38.7
coord: 162..203
e-value: 6.4E-4
score: 29.0
coord: 69..106
e-value: 21.0
score: 8.6
coord: 109..148
e-value: 1.5E-10
score: 51.1
IPR001680WD40 repeatPFAMPF00400WD40coord: 167..203
e-value: 0.0018
score: 19.0
coord: 111..148
e-value: 9.1E-8
score: 32.7
coord: 260..295
e-value: 2.0E-6
score: 28.4
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 116..149
score: 14.585471
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 169..212
score: 9.672996
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 263..304
score: 15.888781
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 215..323
e-value: 1.0E-15
score: 59.8
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 2..214
e-value: 1.0E-32
score: 115.2
NoneNo IPR availablePANTHERPTHR18763WD-REPEAT PROTEIN 18coord: 2..433
NoneNo IPR availablePANTHERPTHR18763:SF0WD REPEAT-CONTAINING PROTEIN 18coord: 2..433
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 263..298
score: 12.130133
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 116..149
score: 12.051043
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 282..296
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 8..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002473.1MS002473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding