MS002461 (gene) Bitter gourd (TR) v1

Overview
NameMS002461
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase superfamily protein
Locationscaffold318: 264002 .. 265483 (-)
RNA-Seq ExpressionMS002461
SyntenyMS002461
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTAGCAGTGAAGCAGCTTGATAGAAATGGATTGCAAGGGAACAGAGAGTTTCTTGTGGAGGTTTTGATGCTAAGTCTGTTGCACCATCAAAACCTTGTCAATCTCATTGGCTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTTTAGAAGATCATCTACTTGGTAATCATTATCCTCAAAACCCATTTCAAAAATTTAGCTAACATTTCAAATGTGTTCTTAGACAGAGAAAACATTGATATAAGGTATATGCTATAGGTTCAATTCTTCTAACACTTCTTTCTTAAATAATATTAGTTTGAATTCAGTCCGGTTGTCAAGAGTTTGTGGTCTCTTGGTCATACTAACTTAAAGGTTTTAAGTTCGAACTTTCTGATGAGCTTAATACCAAAAGTCTATGATGTGTCCCGAATCTTGGAGCGGGTGCGGATGCTTCTGGTCATCGGTGGAGCAAAACTCTAACTCCCAGTTATTAAAATAAAAAAAATTTGAATCACAAAACTCAATACATGAACTTTCCAATTATCAAAATATAGAAGAGATATACTACCTTAGAATTCAAAGTACACCCCGTTGAATCACTAATAAATTTGAAAGCTTACACTATTAAGGCTAAATGTATGTCTTTCTTCAATTTAGTCCTTCTATTTCAACAATTTTCAATTACGAATGCATTAAAACTGGTTGGTTGATGATAAGCTTAAACAACATGATATACCTAGAGAATTGGTAGAACTTCTAAAAAGGAATTGGGCTAAGTTAAAGAGAGGAAAAGCTGAAGCTTTCCTTGCACAAAAATAGTGCCAATTCATGATCTCTTATAATTCAATATGCATGTCATGTACTAATACGTTGTTAAGGAATGCGGTACCTTCAAAGTATCTCAAAAATGGTATAATATTGTTCCCTAATGGAGATGTTATCTCTCATTTATATACATATAATCTTTCCCTTATCTAATCAATGTAAGACTTTAGTCGTAATCGATATAATTATCAAATAAATGTTAAGTATATGATCGAGTAACTTGTAAAAGTTATGACTGAAAAACAGACCTTCCAGCGGAGAAAAGGGCATTGGATTGGGGGACGAGGATGAAAGTAGGGTTGGGAGCAGCAAAAGGGCTGGAATATCTACACGACAAGGCCAACCCACCAGTGATATACAGAGATTTGAAGGCATCAAACATTCTTTTGGACAGCGAATTCAACGCAAAGCTGTCAGATTTCGGGCTGGCGAAGCTCGGGCCTGTCGGGGACAAGCAGCATGTTTCCTCCAGAGTTATGGGAACCTATGGATACTGTGCTCCTGAGTACCAGAGGACTGGCCAGCTTACTCTCAAGTCTGATGTCTACAGCTTTGGCGTTGTGTTGTTGGAACTCATCACTGGCAAGAGAGTCATTGACAACACTCGCCCACCTAAACAGCAGAACTTGGTGGCTTGGGTACGTGAT

mRNA sequence

GTTGTAGCAGTGAAGCAGCTTGATAGAAATGGATTGCAAGGGAACAGAGAGTTTCTTGTGGAGGTTTTGATGCTAAGTCTGTTGCACCATCAAAACCTTGTCAATCTCATTGGCTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTTTAGAAGATCATCTACTTGCGGAGAAAAGGGCATTGGATTGGGGGACGAGGATGAAAGTAGGGTTGGGAGCAGCAAAAGGGCTGGAATATCTACACGACAAGGCCAACCCACCAGTGATATACAGAGATTTGAAGGCATCAAACATTCTTTTGGACAGCGAATTCAACGCAAAGCTGTCAGATTTCGGGCTGGCGAAGCTCGGGCCTGTCGGGGACAAGCAGCATGTTTCCTCCAGAGTTATGGGAACCTATGGATACTGTGCTCCTGAGTACCAGAGGACTGGCCAGCTTACTCTCAAGTCTGATGTCTACAGCTTTGGCGTTGTGTTGTTGGAACTCATCACTGGCAAGAGAGTCATTGACAACACTCGCCCACCTAAACAGCAGAACTTGGTGGCTTGGGTACGTGAT

Coding sequence (CDS)

GTTGTAGCAGTGAAGCAGCTTGATAGAAATGGATTGCAAGGGAACAGAGAGTTTCTTGTGGAGGTTTTGATGCTAAGTCTGTTGCACCATCAAAACCTTGTCAATCTCATTGGCTATTGTGCAGATGGTGATCAAAGACTCTTGGTCTATGAGTATATGCCTTTGGGATCTTTAGAAGATCATCTACTTGCGGAGAAAAGGGCATTGGATTGGGGGACGAGGATGAAAGTAGGGTTGGGAGCAGCAAAAGGGCTGGAATATCTACACGACAAGGCCAACCCACCAGTGATATACAGAGATTTGAAGGCATCAAACATTCTTTTGGACAGCGAATTCAACGCAAAGCTGTCAGATTTCGGGCTGGCGAAGCTCGGGCCTGTCGGGGACAAGCAGCATGTTTCCTCCAGAGTTATGGGAACCTATGGATACTGTGCTCCTGAGTACCAGAGGACTGGCCAGCTTACTCTCAAGTCTGATGTCTACAGCTTTGGCGTTGTGTTGTTGGAACTCATCACTGGCAAGAGAGTCATTGACAACACTCGCCCACCTAAACAGCAGAACTTGGTGGCTTGGGTACGTGAT

Protein sequence

VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLSDFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVIDNTRPPKQQNLVAWVRD
Homology
BLAST of MS002461 vs. NCBI nr
Match: XP_022150776.1 (probable serine/threonine-protein kinase PBL25 [Momordica charantia])

HSP 1 Score: 387.1 bits (993), Expect = 8.9e-104
Identity = 191/194 (98.45%), Postives = 191/194 (98.45%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 126 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 185

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL   AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS
Sbjct: 186 HLLDLPAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 245

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI
Sbjct: 246 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 305

Query: 181 DNTRPPKQQNLVAW 192
           DNTRPPKQQNLVAW
Sbjct: 306 DNTRPPKQQNLVAW 319

BLAST of MS002461 vs. NCBI nr
Match: XP_038899485.1 (probable serine/threonine-protein kinase PBL25 [Benincasa hispida])

HSP 1 Score: 374.8 bits (961), Expect = 4.5e-100
Identity = 184/194 (94.85%), Postives = 188/194 (96.91%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 132 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 191

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL   AE++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 192 HLLDLPAERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 251

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 252 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 311

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 312 DNTRPAKQQNLVAW 325

BLAST of MS002461 vs. NCBI nr
Match: TYK21491.1 (serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 372.5 bits (955), Expect = 2.3e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 214 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 273

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 274 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 333

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 334 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 393

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 394 DNTRPAKQQNLVAW 407

BLAST of MS002461 vs. NCBI nr
Match: KAA0026298.1 (serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 372.5 bits (955), Expect = 2.3e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 60  VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 119

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 120 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 179

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 180 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 239

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 240 DNTRPAKQQNLVAW 253

BLAST of MS002461 vs. NCBI nr
Match: XP_008458110.2 (PREDICTED: serine/threonine-protein kinase At3g07070 [Cucumis melo])

HSP 1 Score: 372.5 bits (955), Expect = 2.3e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 231 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 290

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 291 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 350

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 351 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 410

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 411 DNTRPAKQQNLVAW 424

BLAST of MS002461 vs. ExPASy Swiss-Prot
Match: Q9SFT7 (Probable serine/threonine-protein kinase PBL26 OS=Arabidopsis thaliana OX=3702 GN=PBL26 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.6e-92
Identity = 164/196 (83.67%), Postives = 178/196 (90.82%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           +VAVKQLDRNGLQGN+EF+VEVLMLSLLHH++LVNLIGYCADGDQRLLVYEYM  GSLED
Sbjct: 104 IVAVKQLDRNGLQGNKEFIVEVLMLSLLHHKHLVNLIGYCADGDQRLLVYEYMSRGSLED 163

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    ++  LDW TR+++ LGAA GLEYLHDKANPPVIYRDLKA+NILLD EFNAKLS
Sbjct: 164 HLLDLTPDQIPLDWDTRIRIALGAAMGLEYLHDKANPPVIYRDLKAANILLDGEFNAKLS 223

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITG+RVI
Sbjct: 224 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTTKSDVYSFGVVLLELITGRRVI 283

Query: 181 DNTRPPKQQNLVAWVR 194
           D TRP  +QNLV W +
Sbjct: 284 DTTRPKDEQNLVTWAQ 299

BLAST of MS002461 vs. ExPASy Swiss-Prot
Match: Q9LRY1 (Probable serine/threonine-protein kinase PBL25 OS=Arabidopsis thaliana OX=3702 GN=PBL25 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 1.7e-89
Identity = 161/194 (82.99%), Postives = 174/194 (89.69%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQG REFLVEVLMLSLLHH+NLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 90  VVAVKQLDRNGLQGQREFLVEVLMLSLLHHRNLVNLIGYCADGDQRLLVYEYMPLGSLED 149

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL     ++ LDW TR+K+ LGAAKG+EYLHD+A+PPVIYRDLK+SNILLD E+ AKLS
Sbjct: 150 HLLDLEPGQKPLDWNTRIKIALGAAKGIEYLHDEADPPVIYRDLKSSNILLDPEYVAKLS 209

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGD  HVSSRVMGTYGYCAPEYQRTG LT KSDVYSFGVVLLELI+G+RVI
Sbjct: 210 DFGLAKLGPVGDTLHVSSRVMGTYGYCAPEYQRTGYLTNKSDVYSFGVVLLELISGRRVI 269

Query: 181 DNTRPPKQQNLVAW 192
           D  RP  +QNLV W
Sbjct: 270 DTMRPSHEQNLVTW 283

BLAST of MS002461 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 1.7e-89
Identity = 160/196 (81.63%), Postives = 170/196 (86.73%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           +VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 108 IVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLED 167

Query: 61  H---LLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           H   L  +K  LDW TRM +  GAAKGLEYLHDKANPPVIYRDLK+SNILL   ++ KLS
Sbjct: 168 HLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLS 227

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFGVV LELITG++ I
Sbjct: 228 DFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAI 287

Query: 181 DNTRPPKQQNLVAWVR 194
           DN R P + NLVAW R
Sbjct: 288 DNARAPGEHNLVAWAR 303

BLAST of MS002461 vs. ExPASy Swiss-Prot
Match: Q6I5Q6 (Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK185 PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 8.4e-89
Identity = 158/195 (81.03%), Postives = 171/195 (87.69%), Query Frame = 0

Query: 2   VAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLEDH 61
           VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYE+MPLGSLEDH
Sbjct: 105 VAVKQLDRNGLQGNREFLVEVLMLSLLHHDNLVNLIGYCADGDQRLLVYEFMPLGSLEDH 164

Query: 62  L---LAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLSD 121
           L     +K  LDW TRMK+  GAAKGLE+LHDKANPPVIYRD K+SNILL   ++ KLSD
Sbjct: 165 LHDIPPDKEPLDWNTRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILLGEGYHPKLSD 224

Query: 122 FGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVID 181
           FGLAKLGPVGDK HVS+RVMGTYGYCAPEY  TGQLT+KSDVYSFGVV LELITG++ ID
Sbjct: 225 FGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAID 284

Query: 182 NTRPPKQQNLVAWVR 194
           NT+P  +QNLVAW R
Sbjct: 285 NTKPLGEQNLVAWAR 299

BLAST of MS002461 vs. ExPASy Swiss-Prot
Match: Q9FE20 (Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 2.4e-88
Identity = 158/196 (80.61%), Postives = 170/196 (86.73%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYE+MPLGSLED
Sbjct: 111 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLED 170

Query: 61  H---LLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           H   L  +K ALDW  RMK+  GAAKGLE+LHDKANPPVIYRD K+SNILLD  F+ KLS
Sbjct: 171 HLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLS 230

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGP GDK HVS+RVMGTYGYCAPEY  TGQLT+KSDVYSFGVV LELITG++ I
Sbjct: 231 DFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAI 290

Query: 181 DNTRPPKQQNLVAWVR 194
           D+  P  +QNLVAW R
Sbjct: 291 DSEMPHGEQNLVAWAR 306

BLAST of MS002461 vs. ExPASy TrEMBL
Match: A0A6J1DCI7 (probable serine/threonine-protein kinase PBL25 OS=Momordica charantia OX=3673 GN=LOC111018838 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 4.3e-104
Identity = 191/194 (98.45%), Postives = 191/194 (98.45%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 126 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 185

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL   AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS
Sbjct: 186 HLLDLPAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 245

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI
Sbjct: 246 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 305

Query: 181 DNTRPPKQQNLVAW 192
           DNTRPPKQQNLVAW
Sbjct: 306 DNTRPPKQQNLVAW 319

BLAST of MS002461 vs. ExPASy TrEMBL
Match: A0A5A7SJK0 (Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G003070 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 60  VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 119

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 120 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 179

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 180 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 239

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 240 DNTRPAKQQNLVAW 253

BLAST of MS002461 vs. ExPASy TrEMBL
Match: A0A5D3DCU1 (Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold305G00280 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 214 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 273

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 274 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 333

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 334 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 393

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 394 DNTRPAKQQNLVAW 407

BLAST of MS002461 vs. ExPASy TrEMBL
Match: A0A1S3C8C3 (serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC103497640 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.1e-99
Identity = 183/194 (94.33%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 231 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 290

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 291 HLLDLPFERKALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 350

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 351 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 410

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 411 DNTRPAKQQNLVAW 424

BLAST of MS002461 vs. ExPASy TrEMBL
Match: A0A0A0K554 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G338620 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.2e-99
Identity = 182/194 (93.81%), Postives = 187/194 (96.39%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 137 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 196

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    E++ALDWGTRMKVGLGAA+GLEYLHDKANPPVIYRDLKASNILLD++FNAKLS
Sbjct: 197 HLLDLPFERKALDWGTRMKVGLGAAQGLEYLHDKANPPVIYRDLKASNILLDNDFNAKLS 256

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITGKRVI
Sbjct: 257 DFGLAKLGPVGDKSHVSSRVMGTYGYCAPEYQRTGQLTPKSDVYSFGVVLLELITGKRVI 316

Query: 181 DNTRPPKQQNLVAW 192
           DNTRP KQQNLVAW
Sbjct: 317 DNTRPAKQQNLVAW 330

BLAST of MS002461 vs. TAIR 10
Match: AT3G07070.1 (Protein kinase superfamily protein )

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-93
Identity = 164/196 (83.67%), Postives = 178/196 (90.82%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           +VAVKQLDRNGLQGN+EF+VEVLMLSLLHH++LVNLIGYCADGDQRLLVYEYM  GSLED
Sbjct: 104 IVAVKQLDRNGLQGNKEFIVEVLMLSLLHHKHLVNLIGYCADGDQRLLVYEYMSRGSLED 163

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL    ++  LDW TR+++ LGAA GLEYLHDKANPPVIYRDLKA+NILLD EFNAKLS
Sbjct: 164 HLLDLTPDQIPLDWDTRIRIALGAAMGLEYLHDKANPPVIYRDLKAANILLDGEFNAKLS 223

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLT KSDVYSFGVVLLELITG+RVI
Sbjct: 224 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTTKSDVYSFGVVLLELITGRRVI 283

Query: 181 DNTRPPKQQNLVAWVR 194
           D TRP  +QNLV W +
Sbjct: 284 DTTRPKDEQNLVTWAQ 299

BLAST of MS002461 vs. TAIR 10
Match: AT3G24790.1 (Protein kinase superfamily protein )

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-90
Identity = 161/194 (82.99%), Postives = 174/194 (89.69%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQG REFLVEVLMLSLLHH+NLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 72  VVAVKQLDRNGLQGQREFLVEVLMLSLLHHRNLVNLIGYCADGDQRLLVYEYMPLGSLED 131

Query: 61  HLL---AEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           HLL     ++ LDW TR+K+ LGAAKG+EYLHD+A+PPVIYRDLK+SNILLD E+ AKLS
Sbjct: 132 HLLDLEPGQKPLDWNTRIKIALGAAKGIEYLHDEADPPVIYRDLKSSNILLDPEYVAKLS 191

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGD  HVSSRVMGTYGYCAPEYQRTG LT KSDVYSFGVVLLELI+G+RVI
Sbjct: 192 DFGLAKLGPVGDTLHVSSRVMGTYGYCAPEYQRTGYLTNKSDVYSFGVVLLELISGRRVI 251

Query: 181 DNTRPPKQQNLVAW 192
           D  RP  +QNLV W
Sbjct: 252 DTMRPSHEQNLVTW 265

BLAST of MS002461 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-90
Identity = 160/196 (81.63%), Postives = 170/196 (86.73%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           +VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 108 IVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLED 167

Query: 61  H---LLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           H   L  +K  LDW TRM +  GAAKGLEYLHDKANPPVIYRDLK+SNILL   ++ KLS
Sbjct: 168 HLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLS 227

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFGVV LELITG++ I
Sbjct: 228 DFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAI 287

Query: 181 DNTRPPKQQNLVAWVR 194
           DN R P + NLVAW R
Sbjct: 288 DNARAPGEHNLVAWAR 303

BLAST of MS002461 vs. TAIR 10
Match: AT5G18610.2 (Protein kinase superfamily protein )

HSP 1 Score: 330.1 bits (845), Expect = 1.2e-90
Identity = 160/196 (81.63%), Postives = 170/196 (86.73%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           +VAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYEYMPLGSLED
Sbjct: 108 IVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLED 167

Query: 61  H---LLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           H   L  +K  LDW TRM +  GAAKGLEYLHDKANPPVIYRDLK+SNILL   ++ KLS
Sbjct: 168 HLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLS 227

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGPVGDK HVS+RVMGTYGYCAPEY  TGQLTLKSDVYSFGVV LELITG++ I
Sbjct: 228 DFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAI 287

Query: 181 DNTRPPKQQNLVAWVR 194
           DN R P + NLVAW R
Sbjct: 288 DNARAPGEHNLVAWAR 303

BLAST of MS002461 vs. TAIR 10
Match: AT5G13160.1 (Protein kinase superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 1.7e-89
Identity = 158/196 (80.61%), Postives = 170/196 (86.73%), Query Frame = 0

Query: 1   VVAVKQLDRNGLQGNREFLVEVLMLSLLHHQNLVNLIGYCADGDQRLLVYEYMPLGSLED 60
           VVAVKQLDRNGLQGNREFLVEVLMLSLLHH NLVNLIGYCADGDQRLLVYE+MPLGSLED
Sbjct: 111 VVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLED 170

Query: 61  H---LLAEKRALDWGTRMKVGLGAAKGLEYLHDKANPPVIYRDLKASNILLDSEFNAKLS 120
           H   L  +K ALDW  RMK+  GAAKGLE+LHDKANPPVIYRD K+SNILLD  F+ KLS
Sbjct: 171 HLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLS 230

Query: 121 DFGLAKLGPVGDKQHVSSRVMGTYGYCAPEYQRTGQLTLKSDVYSFGVVLLELITGKRVI 180
           DFGLAKLGP GDK HVS+RVMGTYGYCAPEY  TGQLT+KSDVYSFGVV LELITG++ I
Sbjct: 231 DFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAI 290

Query: 181 DNTRPPKQQNLVAWVR 194
           D+  P  +QNLVAW R
Sbjct: 291 DSEMPHGEQNLVAWAR 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150776.18.9e-10498.45probable serine/threonine-protein kinase PBL25 [Momordica charantia][more]
XP_038899485.14.5e-10094.85probable serine/threonine-protein kinase PBL25 [Benincasa hispida][more]
TYK21491.12.3e-9994.33serine/threonine-protein kinase [Cucumis melo var. makuwa][more]
KAA0026298.12.3e-9994.33serine/threonine-protein kinase [Cucumis melo var. makuwa][more]
XP_008458110.22.3e-9994.33PREDICTED: serine/threonine-protein kinase At3g07070 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9SFT71.6e-9283.67Probable serine/threonine-protein kinase PBL26 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9LRY11.7e-8982.99Probable serine/threonine-protein kinase PBL25 OS=Arabidopsis thaliana OX=3702 G... [more]
Q1PDV61.7e-8981.63Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
Q6I5Q68.4e-8981.03Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9FE202.4e-8880.61Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1DCI74.3e-10498.45probable serine/threonine-protein kinase PBL25 OS=Momordica charantia OX=3673 GN... [more]
A0A5A7SJK01.1e-9994.33Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5D3DCU11.1e-9994.33Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8C31.1e-9994.33serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC10349764... [more]
A0A0A0K5543.2e-9993.81Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G338... [more]
Match NameE-valueIdentityDescription
AT3G07070.11.2e-9383.67Protein kinase superfamily protein [more]
AT3G24790.11.2e-9082.99Protein kinase superfamily protein [more]
AT5G18610.11.2e-9081.63Protein kinase superfamily protein [more]
AT5G18610.21.2e-9081.63Protein kinase superfamily protein [more]
AT5G13160.11.7e-8980.61Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 1..192
e-value: 6.4E-13
score: 51.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..194
score: 32.852375
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..53
e-value: 3.5E-19
score: 70.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 54..194
e-value: 8.4E-49
score: 167.7
NoneNo IPR availablePANTHERPTHR47985OS07G0668900 PROTEINcoord: 1..193
NoneNo IPR availablePANTHERPTHR47985:SF4OS07G0668900 PROTEINcoord: 1..193
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 2..179
e-value: 2.3E-40
score: 138.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 96..108
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 2..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002461.1MS002461.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity