MS002459 (gene) Bitter gourd (TR) v1

BLAST of MS002459 vs. NCBI nr
Match: XP_022143579.1 (ankyrin repeat-containing protein NPR4-like [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-168
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS
Sbjct: 507 LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 566

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD
Sbjct: 567 SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 626

Query: 121 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP 180
           DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP
Sbjct: 627 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP 686

Query: 181 IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS 240
           IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS
Sbjct: 687 IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS 746

Query: 241 IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF 300
           IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF
Sbjct: 747 IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF 806

Query: 301 KRGPRKLLST 311
           KRGPRKLLST
Sbjct: 807 KRGPRKLLST 816

BLAST of MS002459 vs. NCBI nr
Match: XP_038876939.1 (ankyrin repeat-containing protein NPR4-like [Benincasa hispida])

HSP 1 Score: 458.8 bits (1179), Expect = 3.8e-125
Identity = 231/314 (73.57%), Postives = 271/314 (86.31%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE DDDGK++FH+AVENRLENVF+LIHE+  LK+FS
Sbjct: 362 LHDAARAGNVEFLILLIQSYPNIAWEDDDDGKNVFHVAVENRLENVFSLIHEISGLKDFS 421

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ EAKC+
Sbjct: 422 AKYRTTGKEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQREAKCE 481

Query: 121 ----DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN 180
                +LTP ELFT++HKDLRKDGEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+D N
Sbjct: 482 VDSSIKLTPCELFTKEHKDLRKDGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDGN 541

Query: 181 TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLAS 240
           +GIPIFQ + WF +FVI DA A VSSSTSIL F+SILTSRYAEDDFL+SLPS+LL G+AS
Sbjct: 542 SGIPIFQHEFWFTIFVISDAFALVSSSTSILMFLSILTSRYAEDDFLYSLPSKLLVGIAS 601

Query: 241 LFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLS 300
           LFISIVCMV+AFSATFF++Y++ NI +P  + AIA+VP+SCFC L F L  D F NTYLS
Sbjct: 602 LFISIVCMVIAFSATFFMLYNEANIWIPTTVTAIAIVPISCFCALHFGLWMDTFYNTYLS 661

Query: 301 RFLFKRGPRKLLST 311
           R LF+   RKL S+
Sbjct: 662 RLLFRPHQRKLFSS 675

BLAST of MS002459 vs. NCBI nr
Match: XP_022137095.1 (ankyrin repeat-containing protein ITN1-like [Momordica charantia])

HSP 1 Score: 458.4 bits (1178), Expect = 5.0e-125
Identity = 230/310 (74.19%), Postives = 270/310 (87.10%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLI++I  YPD  WE DDDGK IFH+AVENRLENVFNLIHE+  L++FS
Sbjct: 306 LHDAARAGNVEFLIILIRRYPDIVWEEDDDGKGIFHVAVENRLENVFNLIHEISGLRDFS 365

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+K+     YN+LHL AK+AA NHLNRVSGAALQMQRELLWFKEVEKIV+PSQL+AKCD
Sbjct: 366 TKYKTTTKENYNMLHLAAKLAASNHLNRVSGAALQMQRELLWFKEVEKIVLPSQLDAKCD 425

Query: 121 D--QLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTG 180
           D  +LTP ELF+E+HK+LR++GEEWMK+TANSCMLVATLIATVVFAA+FTVPGG D+N+G
Sbjct: 426 DPRKLTPHELFSEEHKNLRREGEEWMKNTANSCMLVATLIATVVFAASFTVPGGFDNNSG 485

Query: 181 IPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLF 240
            PIFQ+ LWF +FVI DA A VSSS+SIL F+SILTSRY EDDFL+SLPS LLFGLASLF
Sbjct: 486 TPIFQQNLWFTIFVISDAAALVSSSSSILMFLSILTSRYTEDDFLYSLPSGLLFGLASLF 545

Query: 241 ISIVCMVLAFSATFFLIYHQ-VNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSR 300
           +SIVCMV+AFSATFF++YH+  ++ VPA I A+A++PVSCFC LQFKL  D F+NTYLSR
Sbjct: 546 VSIVCMVVAFSATFFILYHESADVWVPATIAAMAIIPVSCFCALQFKLWVDTFQNTYLSR 605

Query: 301 FLFKRGPRKL 308
           FLFK   +KL
Sbjct: 606 FLFKPSRQKL 615

BLAST of MS002459 vs. NCBI nr
Match: XP_004153885.1 (ankyrin repeat-containing protein At5g02620 [Cucumis sativus] >KGN64474.1 hypothetical protein Csa_013441 [Cucumis sativus])

HSP 1 Score: 451.1 bits (1159), Expect = 8.0e-123
Identity = 229/314 (72.93%), Postives = 268/314 (85.35%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE DDDGK++FHIAVENRLENVF+LIHE+  LK+FS
Sbjct: 362 LHDAARAGNVEFLILLIQSYPNIAWEDDDDGKNVFHIAVENRLENVFSLIHEISGLKDFS 421

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ  AKC+
Sbjct: 422 AKYRTTGKEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQRGAKCE 481

Query: 121 ----DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN 180
                +LTP ELFT++HKDLRKDGEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+D N
Sbjct: 482 VDSLIKLTPCELFTKEHKDLRKDGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDGN 541

Query: 181 TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLAS 240
           +G PIFQ + WF +FVI DA   VSSSTSIL F+SILTSRYAEDDFLHSLPS+LL G+AS
Sbjct: 542 SGTPIFQHEFWFTIFVISDAFGLVSSSTSILMFLSILTSRYAEDDFLHSLPSKLLVGIAS 601

Query: 241 LFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLS 300
           LFISIVCMV+AFSATFF++Y++ N+ +PA + AIA+VP+SCF  L F L  D F NTYLS
Sbjct: 602 LFISIVCMVIAFSATFFMLYNKKNMWIPATVTAIAIVPISCFFALHFGLWIDTFHNTYLS 661

Query: 301 RFLFKRGPRKLLST 311
           R LF+   RKL S+
Sbjct: 662 RLLFRPHQRKLFSS 675

BLAST of MS002459 vs. NCBI nr
Match: XP_022994345.1 (ankyrin repeat-containing protein ITN1-like [Cucurbita maxima])

HSP 1 Score: 450.7 bits (1158), Expect = 1.0e-122
Identity = 229/313 (73.16%), Postives = 268/313 (85.62%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE D+DGK++FH+AVENRLENVF+LIHE+  LK+FS
Sbjct: 366 LHDAARAGNVEFLILLIRSYPNIAWEDDEDGKNVFHVAVENRLENVFSLIHEISGLKDFS 425

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ EAKC+
Sbjct: 426 AKYRTTGQEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQREAKCE 485

Query: 121 DQ---LTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNT 180
            +   L P ELFT++HKDLRK+GEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+DD  
Sbjct: 486 FESLKLIPSELFTKEHKDLRKEGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDDK- 545

Query: 181 GIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASL 240
           G P+FQR+ WF +FVI DA A VSSSTSIL F+SILTSRYAEDDFLHSLPS+LLFG+ASL
Sbjct: 546 GTPVFQREFWFTIFVISDAFALVSSSTSILMFLSILTSRYAEDDFLHSLPSKLLFGIASL 605

Query: 241 FISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSR 300
           FISIVCMV+AFSATFF++Y   N+ +P  + AIA+VP+SCF  LQF L  D F NTYLSR
Sbjct: 606 FISIVCMVIAFSATFFMLYDGTNVWIPTTVTAIAIVPISCFFALQFGLWMDTFYNTYLSR 665

Query: 301 FLFKRGPRKLLST 311
            LFK   RKL S+
Sbjct: 666 LLFKPHQRKLFSS 677

BLAST of MS002459 vs. ExPASy Swiss-Prot
Match: A2CIR5 (Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 GN=NPR4 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.7e-06
Identity = 38/137 (27.74%), Postives = 78/137 (56.93%), Query Frame = 0

Query: 134 KDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIPIFQRKLWFNVFVI 193
           K+LRK   E + +  NS  +VA L ATV FAA FTVPGG+ +N G+ +  +   F +F I
Sbjct: 397 KELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGNANN-GVAVVVQAASFRIFFI 456

Query: 194 MDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFISIVCMVLAFSATFF 253
            +A+A  +S   ++  ++++      +  +  + ++L      ++++ VC  ++F A+ +
Sbjct: 457 FNAIALFTSLAVVVVQITVVRGETKSERKVVEVINKL------MWLASVCTTISFIASCY 516

Query: 254 LIYHQVNIQVPAMIVAI 271
           ++  + + Q  A++V++
Sbjct: 517 IVLGR-HFQWAALLVSL 525

BLAST of MS002459 vs. ExPASy Swiss-Prot
Match: Q9C7A2 (Ankyrin repeat-containing protein ITN1 OS=Arabidopsis thaliana OX=3702 GN=ITN1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.4e-06
Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 134 KDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIPIFQRKLWFNVFVI 193
           K+LRK   E + +  NS  +VA L ATV FAA FTVPGG D+N G  +   +  F +F I
Sbjct: 405 KELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGG-DNNDGSAVVVGRASFKIFFI 464

Query: 194 MDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFISIVCMVLAFSATFF 253
            +A+A  +S   ++  ++++      +  +  + ++L++ LAS+     C  +AF A+ +
Sbjct: 465 FNALALFTSLAVVVVQITLVRGETKAEKRVVEVINKLMW-LASM-----CTSVAFLASSY 524

Query: 254 LIYHQVNIQVPAMIVAIAMV 274
           ++  + N     ++  +  V
Sbjct: 525 IVVGRKNEWAAELVTVVGGV 537

BLAST of MS002459 vs. ExPASy Swiss-Prot
Match: Q6AWW5 (Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=At5g02620 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.7e-04
Identity = 65/265 (24.53%), Postives = 120/265 (45.28%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAV-ENRLENVFNLI-HEVVSLKE 60
           LH A +  N E + +++ +        D+ G +  HIAV +NR E V  ++ +  VS   
Sbjct: 197 LHMAVKGQNTEIVDVLMEADGSLINSADNKGNTPLHIAVRKNRAEIVQTVLKYCEVSRVA 256

Query: 61  FSSKFKSVI-----TGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPS 120
            +   ++ +     TG + I+ L+ KI   N  +      ++        KE    +   
Sbjct: 257 VNKSGETALDIAEKTGLHEIVPLLQKIGMQNARSIKPAEKVEPSGSSRKLKETVSEI-GH 316

Query: 121 QLEAKCDDQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGG- 180
           ++  + +     R       K + K   E + +  NS  LVA LIATV FAA F VPG  
Sbjct: 317 EVHTQLEQTGRTRREIQGIAKRVNKMHTEGLNNAINSTTLVAILIATVAFAAIFNVPGQY 376

Query: 181 SDDNTGIP--------IFQRKLWFNVFVIMDAVA-FVSSSTSILTFMSILTSRYAEDDFL 240
           +DD   +P            +  F +FV+ D+ A F+S +  ++    ++  R A+   +
Sbjct: 377 TDDPKDVPPGYSLGEARAAPRPEFLIFVVFDSFALFISLAVVVVQTSVVVIERRAKKQMM 436

Query: 241 HSLPSRLLFGLASLFISIVCMVLAF 249
            ++ ++L++ +A + IS+  + L+F
Sbjct: 437 -AIINKLMW-MACIMISVAFVSLSF 458

BLAST of MS002459 vs. ExPASy TrEMBL
Match: A0A6J1CPQ7 (ankyrin repeat-containing protein NPR4-like OS=Momordica charantia OX=3673 GN=LOC111013443 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.8e-168
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS
Sbjct: 507 LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 566

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD
Sbjct: 567 SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 626

Query: 121 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP 180
           DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP
Sbjct: 627 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGIP 686

Query: 181 IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS 240
           IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS
Sbjct: 687 IFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFIS 746

Query: 241 IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF 300
           IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF
Sbjct: 747 IVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFLF 806

Query: 301 KRGPRKLLST 311
           KRGPRKLLST
Sbjct: 807 KRGPRKLLST 816

BLAST of MS002459 vs. ExPASy TrEMBL
Match: A0A6J1C5Q4 (ankyrin repeat-containing protein ITN1-like OS=Momordica charantia OX=3673 GN=LOC111008655 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.4e-125
Identity = 230/310 (74.19%), Postives = 270/310 (87.10%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLI++I  YPD  WE DDDGK IFH+AVENRLENVFNLIHE+  L++FS
Sbjct: 306 LHDAARAGNVEFLIILIRRYPDIVWEEDDDGKGIFHVAVENRLENVFNLIHEISGLRDFS 365

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+K+     YN+LHL AK+AA NHLNRVSGAALQMQRELLWFKEVEKIV+PSQL+AKCD
Sbjct: 366 TKYKTTTKENYNMLHLAAKLAASNHLNRVSGAALQMQRELLWFKEVEKIVLPSQLDAKCD 425

Query: 121 D--QLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTG 180
           D  +LTP ELF+E+HK+LR++GEEWMK+TANSCMLVATLIATVVFAA+FTVPGG D+N+G
Sbjct: 426 DPRKLTPHELFSEEHKNLRREGEEWMKNTANSCMLVATLIATVVFAASFTVPGGFDNNSG 485

Query: 181 IPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLF 240
            PIFQ+ LWF +FVI DA A VSSS+SIL F+SILTSRY EDDFL+SLPS LLFGLASLF
Sbjct: 486 TPIFQQNLWFTIFVISDAAALVSSSSSILMFLSILTSRYTEDDFLYSLPSGLLFGLASLF 545

Query: 241 ISIVCMVLAFSATFFLIYHQ-VNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSR 300
           +SIVCMV+AFSATFF++YH+  ++ VPA I A+A++PVSCFC LQFKL  D F+NTYLSR
Sbjct: 546 VSIVCMVVAFSATFFILYHESADVWVPATIAAMAIIPVSCFCALQFKLWVDTFQNTYLSR 605

Query: 301 FLFKRGPRKL 308
           FLFK   +KL
Sbjct: 606 FLFKPSRQKL 615

BLAST of MS002459 vs. ExPASy TrEMBL
Match: A0A0A0LU19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G058090 PE=4 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 3.9e-123
Identity = 229/314 (72.93%), Postives = 268/314 (85.35%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE DDDGK++FHIAVENRLENVF+LIHE+  LK+FS
Sbjct: 362 LHDAARAGNVEFLILLIQSYPNIAWEDDDDGKNVFHIAVENRLENVFSLIHEISGLKDFS 421

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ  AKC+
Sbjct: 422 AKYRTTGKEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQRGAKCE 481

Query: 121 ----DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN 180
                +LTP ELFT++HKDLRKDGEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+D N
Sbjct: 482 VDSLIKLTPCELFTKEHKDLRKDGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDGN 541

Query: 181 TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLAS 240
           +G PIFQ + WF +FVI DA   VSSSTSIL F+SILTSRYAEDDFLHSLPS+LL G+AS
Sbjct: 542 SGTPIFQHEFWFTIFVISDAFGLVSSSTSILMFLSILTSRYAEDDFLHSLPSKLLVGIAS 601

Query: 241 LFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLS 300
           LFISIVCMV+AFSATFF++Y++ N+ +PA + AIA+VP+SCF  L F L  D F NTYLS
Sbjct: 602 LFISIVCMVIAFSATFFMLYNKKNMWIPATVTAIAIVPISCFFALHFGLWIDTFHNTYLS 661

Query: 301 RFLFKRGPRKLLST 311
           R LF+   RKL S+
Sbjct: 662 RLLFRPHQRKLFSS 675

BLAST of MS002459 vs. ExPASy TrEMBL
Match: A0A6J1K0Y7 (ankyrin repeat-containing protein ITN1-like OS=Cucurbita maxima OX=3661 GN=LOC111490094 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 5.0e-123
Identity = 229/313 (73.16%), Postives = 268/313 (85.62%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE D+DGK++FH+AVENRLENVF+LIHE+  LK+FS
Sbjct: 366 LHDAARAGNVEFLILLIRSYPNIAWEDDEDGKNVFHVAVENRLENVFSLIHEISGLKDFS 425

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ EAKC+
Sbjct: 426 AKYRTTGQEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQREAKCE 485

Query: 121 DQ---LTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNT 180
            +   L P ELFT++HKDLRK+GEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+DD  
Sbjct: 486 FESLKLIPSELFTKEHKDLRKEGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDDK- 545

Query: 181 GIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASL 240
           G P+FQR+ WF +FVI DA A VSSSTSIL F+SILTSRYAEDDFLHSLPS+LLFG+ASL
Sbjct: 546 GTPVFQREFWFTIFVISDAFALVSSSTSILMFLSILTSRYAEDDFLHSLPSKLLFGIASL 605

Query: 241 FISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSR 300
           FISIVCMV+AFSATFF++Y   N+ +P  + AIA+VP+SCF  LQF L  D F NTYLSR
Sbjct: 606 FISIVCMVIAFSATFFMLYDGTNVWIPTTVTAIAIVPISCFFALQFGLWMDTFYNTYLSR 665

Query: 301 FLFKRGPRKLLST 311
            LFK   RKL S+
Sbjct: 666 LLFKPHQRKLFSS 677

BLAST of MS002459 vs. ExPASy TrEMBL
Match: A0A5A7UM89 (Ankyrin repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G002310 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 8.6e-123
Identity = 228/314 (72.61%), Postives = 268/314 (85.35%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           LHDAARAGNVEFLIL+I SYP+ AWE DDDGK++FH+AVENRLENVF+LIHE+  LK+FS
Sbjct: 368 LHDAARAGNVEFLILLIQSYPNIAWEDDDDGKNVFHVAVENRLENVFSLIHEISGLKDFS 427

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           +K+++    KYN+LHL AK+AAPNHLNRVSGAALQMQRELLWFKEVEKIV+PSQ  AKC+
Sbjct: 428 AKYRTTGKEKYNMLHLAAKLAAPNHLNRVSGAALQMQRELLWFKEVEKIVLPSQRGAKCE 487

Query: 121 ----DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN 180
                +LTP ELFT +HKDLRK+GEEWMK+TANSCMLV+TLIATVVFAAAFTVPGG+D N
Sbjct: 488 VDSLTKLTPCELFTGEHKDLRKEGEEWMKNTANSCMLVSTLIATVVFAAAFTVPGGNDGN 547

Query: 181 TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLAS 240
           +G PIFQ + WF +FVI DA A VSSSTSIL F+SILTSRYAEDDFLHSLPS+LL G+AS
Sbjct: 548 SGTPIFQHEFWFTIFVISDAFALVSSSTSILMFLSILTSRYAEDDFLHSLPSKLLVGIAS 607

Query: 241 LFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLS 300
           LFISIVCMV+AFSATFF++Y++ NI +PA + AIA+VP+SCF  L F L  D F NTYLS
Sbjct: 608 LFISIVCMVIAFSATFFMLYNKENIWIPATVTAIAIVPISCFFALHFGLWIDTFHNTYLS 667

Query: 301 RFLFKRGPRKLLST 311
           R LF+   +KL S+
Sbjct: 668 RLLFRPHQQKLFSS 681

BLAST of MS002459 vs. TAIR 10
Match: AT5G35810.1 (Ankyrin repeat family protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.1e-75
Identity = 150/305 (49.18%), Postives = 211/305 (69.18%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           L DAA++GN+E L+++I SYPD  W  D   +S+FHIA  NR E +FN I+E+ ++K+  
Sbjct: 35  LFDAAQSGNLELLLILIRSYPDLIWTVDHKNQSLFHIAAINRHEKIFNRIYELGAIKDLI 94

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           + +K   +   N+LHLVA++  PN L  VSGAALQMQRE+LW+K V++IV    ++ K  
Sbjct: 95  AMYKEKESND-NLLHLVARLPPPNRLQVVSGAALQMQREILWYKAVKEIVPRVYIKTKNK 154

Query: 121 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN---- 180
            +    +LFT++H +LRK+GE+WMK TA +C+LV+TLIATVVFAAAFT+PGG+D +    
Sbjct: 155 KEEVAHDLFTKEHDNLRKEGEKWMKETATACILVSTLIATVVFAAAFTLPGGNDTSGDIK 214

Query: 181 -TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLA 240
             G P F+++ WF VF+I D+VA +SS TSI+ F+SILTSRYAE  F  +LP++L+ GL 
Sbjct: 215 TLGFPTFRKEFWFEVFIISDSVALLSSVTSIMIFLSILTSRYAEASFQTTLPTKLMLGLL 274

Query: 241 SLFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYL 300
           +LF+SI+ MVLAF+AT  LI  Q       ++V +A      F  L F+L  D  R+ YL
Sbjct: 275 ALFVSIISMVLAFTATLILIRDQEPKWSLILLVYVASATALSFVVLHFQLWFDTLRSAYL 334

BLAST of MS002459 vs. TAIR 10
Match: AT3G54070.1 (Ankyrin repeat family protein )

HSP 1 Score: 258.1 bits (658), Expect = 9.2e-69
Identity = 145/314 (46.18%), Postives = 207/314 (65.92%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           L DAA  GNVE L+++I S+ D  W  D++ +++FH+A   R EN+F+LI+E+  +K+  
Sbjct: 259 LFDAAELGNVEILVILIRSHLDLLWIVDNNNRTLFHVAALYRHENIFSLIYELGGIKDLI 318

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCD 120
           + +K     K  +LHLVA++   N     SGAAL MQ+ELLWFK V++IV  S +E K  
Sbjct: 319 ASYKEK-QSKDTLLHLVARLPPMNRQQVGSGAALHMQKELLWFKAVKEIVPRSYIETKNT 378

Query: 121 DQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDN---- 180
                 ++FTEQH++LRK+GE WMK TA +CML ATLIATVVFAAA T+PGG+DD+    
Sbjct: 379 KGELAHDIFTEQHENLRKEGERWMKETATACMLGATLIATVVFAAAITIPGGNDDSGDKA 438

Query: 181 --TGIPIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGL 240
              G P F+++L F++F + D+VA  SS  SI+ F+SI TSRYAE+DF + LP++L+FGL
Sbjct: 439 NTLGFPNFRKRLLFDIFTLSDSVALFSSMMSIVIFLSIFTSRYAEEDFRYDLPTKLMFGL 498

Query: 241 ASLFISIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTY 300
           ++LFISI+ M+LAF+ +  LI  +       +I  +A +    F +L F L  +  R+ Y
Sbjct: 499 SALFISIISMILAFTFSMILIRVEKASLSLVLISCLASLTALTFAYLYFHLWFNTLRSVY 558

Query: 301 LSRFLF---KRGPR 306
           +S FLF   KRG R
Sbjct: 559 ISMFLFLGRKRGIR 571

BLAST of MS002459 vs. TAIR 10
Match: AT5G04730.1 (Ankyrin-repeat containing protein )

HSP 1 Score: 221.5 bits (563), Expect = 9.6e-58
Identity = 127/303 (41.91%), Postives = 185/303 (61.06%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECD-DDGKSIFHIAVENRLENVFNLIHEVVSLKEF 60
           L +AA++GN +F I II       W  +   G+++F +AVE + E +FNLIH +   K  
Sbjct: 298 LLEAAKSGNRDFFIEIIKCNSQLLWILNPTSGRNLFQLAVEFKKEKIFNLIHGLDDRK-- 357

Query: 61  SSKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKC 120
            +  +S   G  NILH+  +++ P+ L+++SGAAL+MQRE  WFKEVE +V   ++  K 
Sbjct: 358 VTLLRSYDKGNNNILHIAGRLSTPDQLSKISGAALKMQRESQWFKEVESLVSEREVVQKN 417

Query: 121 DDQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGI 180
            D  TPR++F   H+ LRK+GEEWMK TA +C  VA LIATV F A FTVPGG D  +G 
Sbjct: 418 KDNKTPRQIFEHYHEHLRKEGEEWMKYTATACSFVAALIATVTFQAIFTVPGGIDGTSGS 477

Query: 181 PIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFI 240
           P+    L F  F+  D +AF +S  S+L F+SILTSRY+ DDF+ SLP +++ G + LFI
Sbjct: 478 PLILNDLHFRAFIFTDTLAFFASCISVLIFLSILTSRYSFDDFIVSLPRKMILGQSILFI 537

Query: 241 SIVCMVLAFSATFFL-IYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRF 300
           SI  M++AF  +    + H+  +  P  +  +A  P   F  LQ+ LL ++  +TY  R 
Sbjct: 538 SIASMLVAFITSLSASMRHKPALVYP--LKPLASFPSLLFLMLQYPLLKEMISSTYGKRL 596

Query: 301 LFK 302
            ++
Sbjct: 598 FYR 596

BLAST of MS002459 vs. TAIR 10
Match: AT3G18670.1 (Ankyrin repeat family protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-57
Identity = 127/308 (41.23%), Postives = 187/308 (60.71%), Query Frame = 0

Query: 1   LHDAARAGNVEFLILIISSYPDFAWECDDDGKSIFHIAVENRLENVFNLIHEVVSLKEFS 60
           L  A   G VE++  ++  YPD  W  +  G +IF  AV  R E +F+LI+ + + K   
Sbjct: 293 LFKAVENGIVEYIEEMMRHYPDIVWSKNSSGLNIFFYAVSQRQEKIFSLIYNIGAKKNIL 352

Query: 61  SKFKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLE-AKC 120
           +    +     N+LH  A  A  + LN + GAALQMQREL WFKEVEK+V P   +    
Sbjct: 353 ATNWDIFHN--NMLHHAAYRAPASRLNLIPGAALQMQRELQWFKEVEKLVQPKHRKMVNL 412

Query: 121 DDQLTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNTGI 180
             + TP+ LFT+QHKDL + GE+WMK TA SC +VA LI T++F++AFTVPGG   + G+
Sbjct: 413 KQKKTPKALFTDQHKDLVEQGEKWMKETATSCTVVAALITTMMFSSAFTVPGGYRSD-GM 472

Query: 181 PIFQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFI 240
           P++  +  F +F+I DA++  +S  S+L F+ IL SRY E+DFL SLP++L+ GL +LF+
Sbjct: 473 PLYIHQHRFKIFLISDAISLFTSCMSLLMFLGILKSRYREEDFLRSLPTKLIVGLLALFL 532

Query: 241 SIVCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTYLSRFL 300
           S+  M++ F  T   +  +    V A  + +A++P+  F  LQF +L +IFR TY     
Sbjct: 533 SMATMIVTFVVTLMTLVGEKISWVSAQFMFLAVIPLGMFVVLQFPVLLEIFRATYCPNVF 592

Query: 301 FKRGPRKL 308
            K  PR++
Sbjct: 593 DK--PRRV 595

BLAST of MS002459 vs. TAIR 10
Match: AT5G04700.1 (Ankyrin repeat family protein )

HSP 1 Score: 205.3 bits (521), Expect = 7.1e-53
Identity = 121/293 (41.30%), Postives = 178/293 (60.75%), Query Frame = 0

Query: 4   AARAGNVEFLILIISSYPDFAWECDDDGKS-IFHIAVENRLENVFNLIHEVVSLKEFSSK 63
           A R GNV+FL+ +I +  +  W       S +F +AVE R E VF+L++ +   K     
Sbjct: 366 AVRYGNVDFLVEMIRNNSELLWSTRTSSSSTLFLLAVEFRQEKVFSLLYGLDDRKYLLLA 425

Query: 64  FKSVITGKYNILHLVAKIAAPNHLNRVSGAALQMQRELLWFKEVEKIVMPSQLEAKCDDQ 123
            K        +LHL    + P+ L+ V GA LQ+QREL WFKEVE+I    + E    ++
Sbjct: 426 DKD--CDGNGVLHLAGFPSPPSKLSSVVGAPLQLQRELQWFKEVERIAPEIEKERVNTEE 485

Query: 124 LTPRELFTEQHKDLRKDGEEWMKSTANSCMLVATLIATVVFAAAFTVPGGSDDNT-GIPI 183
            TP E+FT++H+ LR++ E+WMK TA SC LVA LI TV FAA FTVPGG+DDN+ G P 
Sbjct: 486 QTPIEIFTKEHQGLRQEAEKWMKDTAMSCSLVAALIVTVTFAAVFTVPGGTDDNSKGKPF 545

Query: 184 FQRKLWFNVFVIMDAVAFVSSSTSILTFMSILTSRYAEDDFLHSLPSRLLFGLASLFISI 243
             R   F +F++ D ++  +S TS+L F+ ILT+RY+ DDFL  LP++++ GL+ LF+SI
Sbjct: 546 HLRDRRFIIFIVSDLISCFASCTSVLIFLGILTARYSFDDFLVFLPTKMIAGLSILFVSI 605

Query: 244 VCMVLAFSATFFLIYHQVNIQVPAMIVAIAMVPVSCFCFLQFKLLADIFRNTY 295
             M++AFS+  F +  +    + A  +  A +P   F  LQ+ LL ++  +TY
Sbjct: 606 AAMLIAFSSALFTMMGKEGKWIVAPTILFACLPALLFVLLQYPLLKEMIFSTY 656