MS002436 (gene) Bitter gourd (TR) v1

Overview
NameMS002436
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPectinesterase
Locationscaffold318: 55870 .. 57010 (+)
RNA-Seq ExpressionMS002436
SyntenyMS002436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACCAACTTTTAAAATGGCATCCTCTCAGTATATTGCATGCTTTTTTGCTGTGTCATTGATCATCGACTTTTTTATGCCTGTTTGTAGCATTGTTTTCAATGCTACTGTCTCTTTAGATGGCAGTGGAGACTTCAAAAGCATTGGTGCTGCTATTTCAGCTGCACCCGACAACAGTAATTCTAGATTCTACATTCGTATAAAATCTGGTACATACAATGAGCATATTGAAGTTGAAAGTCAAAAGACTTTCATAACGTTAATAGGTGATAATGCAGCTACTACTGTCATTGTTGATAATCGAAGCAATGGCACTGGTTTTGGAACCAGCCAATCAGCCACTTTCGGTAAGTTGGAGCTAGAGCTATATAAACTTTTTCTTTCCTCTTTCTTCTTCTATCATTCCTTTCAAATATATTAACTGTCGTTGAAACTGTTAACATTTCTTTGCAGCTGTGAGGGGTAACAACTTTATGGCAAAGTCCATAACATTTGAAAATTCTGCTGGACCTAATAATGGTCAAGCAGTTGCTCTTTTAAATGACCAAGCCAACTACATCACATATTATCGATGCGTATTTCTTGGATACCAGGACACACTTTTCATAAATGGAAACCATTTATTTTTCAAAGATTGTGAAATCTATGGGACTGTGGATTTCATTTTCGGAGATGGACTTGCAGTGTTTCAAGATTGCAAGATATACGCACGATCATCAAATAATACTATTACTATTACCGCACAGTCAAAAGAGGCAAAACACCAAAATAGTGGCTTCTCATTTCAAAACTGCAGTGTGATGGCCTCTCCTGAACTTGGGTCAAGAAAAGAACCTAACCTTGAAGTTTATCTTGGTCGGCCCTGGAGAGATTACTCGACAGTAATTTTCATGCAGTCATTTTTAGATTCTGTGGTCAATCCAAAGGGATGGTCGCCATGGTCGCCGGATAGGCCTCTAGACTTATTATTTTATGCAGAGTTTAACAACAGGGGTCCAGGAGCTAATATCTCTGGAAGGATCAACTGGCCGGGTTACCATATAATAAAGAATCCACAAGAAGCCCTTGAATATACTGTGAAAACTTTTATTAATGGAACTATATGGCTACCAGAGACGGGTGTACCATTCAGACCAGAGTTA

mRNA sequence

TCACCAACTTTTAAAATGGCATCCTCTCAGTATATTGCATGCTTTTTTGCTGTGTCATTGATCATCGACTTTTTTATGCCTGTTTGTAGCATTGTTTTCAATGCTACTGTCTCTTTAGATGGCAGTGGAGACTTCAAAAGCATTGGTGCTGCTATTTCAGCTGCACCCGACAACAGTAATTCTAGATTCTACATTCGTATAAAATCTGGTACATACAATGAGCATATTGAAGTTGAAAGTCAAAAGACTTTCATAACGTTAATAGGTGATAATGCAGCTACTACTGTCATTGTTGATAATCGAAGCAATGGCACTGGTTTTGGAACCAGCCAATCAGCCACTTTCGCTGTGAGGGGTAACAACTTTATGGCAAAGTCCATAACATTTGAAAATTCTGCTGGACCTAATAATGGTCAAGCAGTTGCTCTTTTAAATGACCAAGCCAACTACATCACATATTATCGATGCGTATTTCTTGGATACCAGGACACACTTTTCATAAATGGAAACCATTTATTTTTCAAAGATTGTGAAATCTATGGGACTGTGGATTTCATTTTCGGAGATGGACTTGCAGTGTTTCAAGATTGCAAGATATACGCACGATCATCAAATAATACTATTACTATTACCGCACAGTCAAAAGAGGCAAAACACCAAAATAGTGGCTTCTCATTTCAAAACTGCAGTGTGATGGCCTCTCCTGAACTTGGGTCAAGAAAAGAACCTAACCTTGAAGTTTATCTTGGTCGGCCCTGGAGAGATTACTCGACAGTAATTTTCATGCAGTCATTTTTAGATTCTGTGGTCAATCCAAAGGGATGGTCGCCATGGTCGCCGGATAGGCCTCTAGACTTATTATTTTATGCAGAGTTTAACAACAGGGGTCCAGGAGCTAATATCTCTGGAAGGATCAACTGGCCGGGTTACCATATAATAAAGAATCCACAAGAAGCCCTTGAATATACTGTGAAAACTTTTATTAATGGAACTATATGGCTACCAGAGACGGGTGTACCATTCAGACCAGAGTTA

Coding sequence (CDS)

TCACCAACTTTTAAAATGGCATCCTCTCAGTATATTGCATGCTTTTTTGCTGTGTCATTGATCATCGACTTTTTTATGCCTGTTTGTAGCATTGTTTTCAATGCTACTGTCTCTTTAGATGGCAGTGGAGACTTCAAAAGCATTGGTGCTGCTATTTCAGCTGCACCCGACAACAGTAATTCTAGATTCTACATTCGTATAAAATCTGGTACATACAATGAGCATATTGAAGTTGAAAGTCAAAAGACTTTCATAACGTTAATAGGTGATAATGCAGCTACTACTGTCATTGTTGATAATCGAAGCAATGGCACTGGTTTTGGAACCAGCCAATCAGCCACTTTCGCTGTGAGGGGTAACAACTTTATGGCAAAGTCCATAACATTTGAAAATTCTGCTGGACCTAATAATGGTCAAGCAGTTGCTCTTTTAAATGACCAAGCCAACTACATCACATATTATCGATGCGTATTTCTTGGATACCAGGACACACTTTTCATAAATGGAAACCATTTATTTTTCAAAGATTGTGAAATCTATGGGACTGTGGATTTCATTTTCGGAGATGGACTTGCAGTGTTTCAAGATTGCAAGATATACGCACGATCATCAAATAATACTATTACTATTACCGCACAGTCAAAAGAGGCAAAACACCAAAATAGTGGCTTCTCATTTCAAAACTGCAGTGTGATGGCCTCTCCTGAACTTGGGTCAAGAAAAGAACCTAACCTTGAAGTTTATCTTGGTCGGCCCTGGAGAGATTACTCGACAGTAATTTTCATGCAGTCATTTTTAGATTCTGTGGTCAATCCAAAGGGATGGTCGCCATGGTCGCCGGATAGGCCTCTAGACTTATTATTTTATGCAGAGTTTAACAACAGGGGTCCAGGAGCTAATATCTCTGGAAGGATCAACTGGCCGGGTTACCATATAATAAAGAATCCACAAGAAGCCCTTGAATATACTGTGAAAACTTTTATTAATGGAACTATATGGCTACCAGAGACGGGTGTACCATTCAGACCAGAGTTA

Protein sequence

SPTFKMASSQYIACFFAVSLIIDFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL
Homology
[Close]
BLAST of MS002436 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458.4 bits (1178), Expect = 5.6e-125
Identity = 227/337 (67.36%), Postives = 267/337 (79.23%), Query Frame = 0 

Query: 12  IACFFAVSLIIDFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGT 71
           ++CFF +  +  F      I +NATVSLDG GD+ +IGAAI+AAP+NS+SRF I +K G 
Sbjct: 1   MSCFFILFFVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGM 60

Query: 72  YNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFEN 131
           Y E+I++ES KTFITL GD+A+TT IV NRS  TGFGT+ SATF V  N+F+A SITF N
Sbjct: 61  YFEYIKIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSN 120

Query: 132 S--AGPNNGQAVALLN-DQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFG 191
           S  AGP +GQAVALLN +  NY  +Y+CVFLGYQDTLFING  LFFKDCEIYGTVDFIFG
Sbjct: 121 SAGAGPKHGQAVALLNSNNENYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFG 180

Query: 192 DGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVY 251
           + L VFQDC+IYAR  NNT+T+TAQSKE K Q SGFSFQNCSVMASPELG   E    V+
Sbjct: 181 NALVVFQDCRIYARLLNNTVTVTAQSKEYKRQLSGFSFQNCSVMASPELGPVNE--TIVF 240

Query: 252 LGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWP 311
           LGRPWRDYSTVIFM+SFLDSVVNP GWS WS    L LLFYAE+ NRGPGAN+SGR+ WP
Sbjct: 241 LGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWP 300

Query: 312 GYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
           G+H I+N +EA  ++V+ FINGT WLPETGVP+RP+L
Sbjct: 301 GFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]
BLAST of MS002436 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458.0 bits (1177), Expect = 7.3e-125
Identity = 227/337 (67.36%), Postives = 267/337 (79.23%), Query Frame = 0 

Query: 12  IACFFAVSLIIDFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGT 71
           ++CFF +  +  F      I +NATVSLDG GD+ +IGAAI+AAP+NS+SRF I +K G 
Sbjct: 1   MSCFFILFFVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIHVKPGM 60

Query: 72  YNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFEN 131
           Y E+I +ES KTFITL GD+A+TT IV NRS  TGFGT+ SATF V  N+F+A SITF N
Sbjct: 61  YFEYIRIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSN 120

Query: 132 S--AGPNNGQAVALLN-DQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFG 191
           S  AGPN+GQAVALLN +  NY  +Y+CVFLGYQDTLFING  LFFKDCEIYGTVDFIFG
Sbjct: 121 SAGAGPNHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFG 180

Query: 192 DGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVY 251
           + L VFQDC+IYAR  NNT+T+TAQSKE + Q SGFSFQNCSVMASPELG   E    V+
Sbjct: 181 NALVVFQDCRIYARLLNNTVTVTAQSKENQLQLSGFSFQNCSVMASPELGPVNE--TIVF 240

Query: 252 LGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWP 311
           LGRPWRDYSTVIFM+SFLDSVVNP GWS WS    L LLFYAE+ NRGPGAN+SGR+ WP
Sbjct: 241 LGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRGPGANLSGRVKWP 300

Query: 312 GYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
           G+H I+N +EA  ++V+ FINGT WLPETGVP+RP+L
Sbjct: 301 GFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]
BLAST of MS002436 vs. NCBI nr
Match: KAG7019877.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 433.3 bits (1113), Expect = 1.9e-117
Identity = 211/307 (68.73%), Postives = 249/307 (81.11%), Query Frame = 0 

Query: 42  SGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNAATTVIVDNR 101
           +GD+ +IGAAI+AAP+NS+SRF I +K G Y E+I++ES KTFITL GD+A+TT IV NR
Sbjct: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145

Query: 102 SNGTGFGTSQSATFAVRGNNFMAKSITFENS--AGPNNGQAVALLN-DQANYITYYRCVF 161
           S  TGFGT+ SATF V  N+F+A SITF NS  AGP +GQAVALLN +  NY  +Y+CVF
Sbjct: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205

Query: 162 LGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSSNNTITITAQSKEAK 221
           LGYQDTLFING  LFFKDCEIYGTVDFIFG+ L VFQDC+IYAR  NNT+T+TAQSK+  
Sbjct: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265

Query: 222 HQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVVNPKGWSPW 281
            Q SGFSFQNCSVM SPELG   E    V+LGRPWRDYSTVIFM+SFLDSVVNP GWS W
Sbjct: 266 KQLSGFSFQNCSVMVSPELGPVNE--TIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEW 325

Query: 282 SPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFINGTIWLPETG 341
           S    L LLFYAE++NRGPGAN+SGR+ WPG+H I+N +EA  ++V+ FINGT WLPETG
Sbjct: 326 SNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETG 385

Query: 342 VPFRPEL 346
           VP+RP+L
Sbjct: 386 VPYRPDL 390

[Close]
BLAST of MS002436 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 425.2 bits (1092), Expect = 5.2e-115
Identity = 215/340 (63.24%), Postives = 254/340 (74.71%), Query Frame = 0 

Query: 6   MASSQYIACFFAVSLIIDFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYI 65
           M   Q++A F A+S +  F +   + VFNATVSLDGSGD+K+I  AI+AAPDNS +RFYI
Sbjct: 1   MVVFQFVAVFVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFYI 60

Query: 66  RIKSGTYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAK 125
            + +GTYNEH+E+ S KTF+ LIGD+A+TT+IVDNRSN  GF T  SATF V+G NFMAK
Sbjct: 61  HVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMAK 120

Query: 126 SITFENSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDF 185
           SITF+NSAGP NGQAVALLND A+   YY C+FLG+QDTL++ G+               
Sbjct: 121 SITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD--------------- 180

Query: 186 IFGDGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNL 245
                    +DCKIY   SN T+TITAQSKE + ++SGFSFQNCSV  SPELG RK+ N 
Sbjct: 181 ---------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQNF 240

Query: 246 EVYLGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRI 305
           +VYLGRP RDYSTVIFMQSFLD+VVNPKGW  WS   PL+LLFYAEFNNRGPGA+IS RI
Sbjct: 241 DVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKRI 300

Query: 306 NWPGYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
            W GYH+I NP EA +YTVK FI+GT WLPET VPFRP L
Sbjct: 301 KWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]
BLAST of MS002436 vs. NCBI nr
Match: XP_023511662.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408.7 bits (1049), Expect = 5.1e-110
Identity = 207/344 (60.17%), Postives = 253/344 (73.55%), Query Frame = 0 

Query: 6   MASSQYIACFFAVSLIIDFFMPVCSIV----FNATVSLDGSGDFKSIGAAISAAPDNSNS 65
           MA   + AC F       FF  V +++    FNA VSLDGSGDFKSI  AI+AAPDNSN+
Sbjct: 1   MAFLPFAACLFF------FFANVLALIDPARFNAVVSLDGSGDFKSISEAIAAAPDNSNT 60

Query: 66  RFYIRIKSGTYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNN 125
           RFYIR+  GTY  H+E+   K FI LIGDNA TT+IVDNRS+GTGF T+ SAT  VRG+N
Sbjct: 61  RFYIRVTPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVRGHN 120

Query: 126 FMAKSITFENSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYG 185
           FMA+ ++FENSAGP NGQAVA+L D A Y  YY C FL +QDTL+  G   FF++CE+YG
Sbjct: 121 FMAQHLSFENSAGPQNGQAVAVL-DYAKYTVYYDCRFLSFQDTLYSRGQCQFFRECEVYG 180

Query: 186 TVDFIFGDGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRK 245
           TVDFIFGDGLAVFQDC IYAR  ++ IT+TAQSK+     SGFSFQNC+V  SPE+ S K
Sbjct: 181 TVDFIFGDGLAVFQDCNIYARLPSDEITVTAQSKDRSWWKSGFSFQNCTVTVSPEIASSK 240

Query: 246 EPNLEVYLGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANI 305
           +  +++YLGRPWR YSTV+FM+SFLD  V P+GW  W    P++ LFYAEFNNRGPGA+ 
Sbjct: 241 D-KVKIYLGRPWRQYSTVVFMESFLDDNVRPEGWVLWE-GVPINNLFYAEFNNRGPGADT 300

Query: 306 SGRINWPGYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
           + R+NW  +H++ + Q A  +TVK FING+ WLPET VPFR  L
Sbjct: 301 THRVNWSTFHVL-DKQLAKNFTVKFFINGSDWLPETHVPFRTGL 334

[Close]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 143/317 (45.11%), Postives = 206/317 (64.98%), Query Frame = 0 

Query: 27  PVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFIT 86
           PV    +N +V++DG+G+F +I  A+ AAP+ S +RF I IK G Y E++E+  +KT I 
Sbjct: 214 PVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 273

Query: 87  LIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLND 146
            IGD    TVI  NRS   G+ T Q+ T  V+G  ++AK I+F NSAGP   QAVA    
Sbjct: 274 FIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRS 333

Query: 147 QANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSSN- 206
            +++  +YRC F GYQDTL+++    F+++C+IYGT+DFIFG+   VFQ+  +YAR  N 
Sbjct: 334 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 393

Query: 207 -NTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQS 266
            + I  TAQS+    Q +G S  NC ++A+P+L   KE N + YLGRPWR YS  + ++S
Sbjct: 394 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKS 453

Query: 267 FLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTV 326
           F+D +++P GW     D  L+ L+Y E+ N GPGAN++ R+ WPG+  I+N  EA ++TV
Sbjct: 454 FIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTV 513

Query: 327 KTFINGTIWLPETGVPF 342
             FI+G+ WL  TG+PF
Sbjct: 514 GPFIDGSTWLNSTGIPF 528

[Close]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 9.0e-78
Identity = 141/309 (45.63%), Postives = 206/309 (66.67%), Query Frame = 0 

Query: 34  NATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNAA 93
           N  V+ DGSG+ K+I  A++AA     SR+ I IK+GTYNE+IEV+ +   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 94  TTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYITY 153
            T+I  ++S G G  T +SAT AV G+NF+A+ IT  N+AGPNN QAVA L   ++   +
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVA-LRSGSDLSVF 321

Query: 154 YRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARS-SNNTITITA 213
           YRC F GYQDTL+++    F+++C+IYGTVDFIFG+   V Q+C I+AR   N T T+TA
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTA 381

Query: 214 QSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVVNP 273
           Q +   +Q++G    NC V A+ +L    + +++ +LGRPW+ YS  +++++FLDS++NP
Sbjct: 382 QGRTDPNQSTGIIIHNCRVTAASDL-KPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 441

Query: 274 KGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFINGTI 333
            GW  WS D  L+ L+YAE+ N GPG++ + R+ W GYH++ +P +  ++TV  FI G  
Sbjct: 442 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 501

Query: 334 WLPETGVPF 342
           WLP T VPF
Sbjct: 502 WLPATNVPF 506

[Close]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.6e-77
Identity = 142/313 (45.37%), Postives = 200/313 (63.90%), Query Frame = 0 

Query: 34  NATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNAA 93
           +A V+ DG+G+F ++  A+ AAPD S  R+ I IK GTY E++E++ +K  + +IGD   
Sbjct: 208 DAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMD 267

Query: 94  TTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYITY 153
            T+I  NRS   G+ T +SATFAV G  F+A+ ITFEN+AGP   QAVAL +D ++   +
Sbjct: 268 ATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSD-SDLSVF 327

Query: 154 YRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSS--NNTITIT 213
           YRC   GYQDTL+ +    F++DC+I GTVDFIFGD   VFQ+C+I A+    N   +IT
Sbjct: 328 YRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSIT 387

Query: 214 AQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVVN 273
           AQ ++  ++ +G S Q C++ A  +L +    +   YLGRPW+ YS  + MQSFL +V+ 
Sbjct: 388 AQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIR 447

Query: 274 PKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFINGT 333
           P+GW  W+ D  L+ LFY E+ N GPGA +  R+ WPGY +     +A  YTV  FI G 
Sbjct: 448 PEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGN 507

Query: 334 IWLPETGVPFRPE 345
           +WLP TGV +  E
Sbjct: 508 LWLPSTGVKYTAE 519

[Close]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 7.7e-77
Identity = 145/311 (46.62%), Postives = 200/311 (64.31%), Query Frame = 0 

Query: 33  FNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNA 92
           ++ +V+LDG+G+F  I  AI  APD S++RF I IK G Y E++E++ +K  I ++GD  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGI 270

Query: 93  ATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYIT 152
             TVI  NRS   G+ T +SATFAV G  F+A+ ITF+N+AGP   QAVAL +D ++   
Sbjct: 271 DVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSD-SDLSV 330

Query: 153 YYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSS--NNTITI 212
           ++RC   GYQDTL+ +    F+++C I GTVDFIFGDG  VFQ+C+I A+    N   TI
Sbjct: 331 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 390

Query: 213 TAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVV 272
           TAQ ++  +Q SGFS Q  ++ A  +L          YLGRPW+ YS  +F+++ +  VV
Sbjct: 391 TAQGRKDVNQPSGFSIQFSNISADADLVPYLN-TTRTYLGRPWKLYSRTVFIRNNMSDVV 450

Query: 273 NPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFING 332
            P+GW  W+ D  LD LFY EF N GPG+ +S R+ WPGYH+  N  +A  +TV  FI G
Sbjct: 451 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 510

Query: 333 TIWLPETGVPF 342
            +WLP TGV F
Sbjct: 511 NLWLPSTGVTF 519

[Close]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 142/321 (44.24%), Postives = 205/321 (63.86%), Query Frame = 0 

Query: 27  PVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFIT 86
           PV     N  V+ +G+G++ +IG AISAAP++S +RF I IK G Y E+IE+  +KT I 
Sbjct: 200 PVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIM 259

Query: 87  LIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLND 146
            IGD    TVI  NRS   G+    SAT  VRG+ F+AK ++F N AGP   QAVA L  
Sbjct: 260 FIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRS 319

Query: 147 QANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYAR--SS 206
            ++   YYRC F  YQDT++++ +  F+++C+IYGTVDFIFGD   VFQ+C +YAR  + 
Sbjct: 320 SSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNP 379

Query: 207 NNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQS 266
           N  I  TAQ +E   + +G S  +  ++A+P+L    + N + YLGRPW+ YS  + M+S
Sbjct: 380 NQKIIYTAQGRENSREPTGISIISSRILAAPDL-IPVQANFKAYLGRPWQLYSRTVIMKS 439

Query: 267 FLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTV 326
           F+D +V+P GW  W  D  L+ L+Y E+ N GPG+N++ R+ WPG+  I+  +EA +++V
Sbjct: 440 FIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSV 499

Query: 327 KTFINGTIWLPETGVPFRPEL 346
             FI+G  WL  T +PF  +L
Sbjct: 500 GPFIDGNKWLNSTRIPFTLDL 518

[Close]
BLAST of MS002436 vs. ExPASy TrEMBL
Match: A0A6J1C9Z6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.5e-115
Identity = 215/340 (63.24%), Postives = 254/340 (74.71%), Query Frame = 0 

Query: 6   MASSQYIACFFAVSLIIDFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYI 65
           M   Q++A F A+S +  F +   + VFNATVSLDGSGD+K+I  AI+AAPDNS +RFYI
Sbjct: 1   MVVFQFVAVFVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFYI 60

Query: 66  RIKSGTYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAK 125
            + +GTYNEH+E+ S KTF+ LIGD+A+TT+IVDNRSN  GF T  SATF V+G NFMAK
Sbjct: 61  HVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFNVKGRNFMAK 120

Query: 126 SITFENSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDF 185
           SITF+NSAGP NGQAVALLND A+   YY C+FLG+QDTL++ G+               
Sbjct: 121 SITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD--------------- 180

Query: 186 IFGDGLAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNL 245
                    +DCKIY   SN T+TITAQSKE + ++SGFSFQNCSV  SPELG RK+ N 
Sbjct: 181 ---------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPELGPRKDQNF 240

Query: 246 EVYLGRPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRI 305
           +VYLGRP RDYSTVIFMQSFLD+VVNPKGW  WS   PL+LLFYAEFNNRGPGA+IS RI
Sbjct: 241 DVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGPGADISKRI 300

Query: 306 NWPGYHIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
            W GYH+I NP EA +YTVK FI+GT WLPET VPFRP L
Sbjct: 301 KWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]
BLAST of MS002436 vs. ExPASy TrEMBL
Match: A0A5D3BHF7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004200 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.2e-110
Identity = 200/332 (60.24%), Postives = 254/332 (76.51%), Query Frame = 0 

Query: 12  IACFFAVSLIIDFFMPVCSIVFNATVSLDGS-GDFKSIGAAISAAPDNSNSRFYIRIKSG 71
           + CFF    ++ F   + S  FNATVSLDGS  DFK+I AAI+AAPDNSN+RFYIR+  G
Sbjct: 11  VMCFFN---LVPF---IHSFTFNATVSLDGSMNDFKTIAAAIAAAPDNSNTRFYIRVTPG 70

Query: 72  TYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFE 131
           TY+E +++   KTFI LIGDNA TT+IVD+RSN  GF TS SAT  V G+NF+AKS+TFE
Sbjct: 71  TYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGDNFIAKSLTFE 130

Query: 132 NSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDG 191
           NSAGP NGQAVA+L DQA +  Y++C FLG+QDTL++ G   FFK+C++YGTVDFIFG+G
Sbjct: 131 NSAGPQNGQAVAVL-DQARFTAYHKCRFLGFQDTLYVGGKSHFFKECDVYGTVDFIFGNG 190

Query: 192 LAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLG 251
           L +FQDC IYAR  N  IT+TAQSKE  H+ SGFSFQNC+V  S E+ + K  N+++YL 
Sbjct: 191 LTMFQDCNIYAREPNRPITVTAQSKEQLHEESGFSFQNCTVTVSSEIVASK-ANVKIYLD 250

Query: 252 RPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGY 311
           RPWR YS V+FM+SFLD  V P+GWS W P  P + LFY EFNNRGPGA++S R++W G+
Sbjct: 251 RPWRQYSRVVFMESFLDEEVLPEGWSEW-PGVPTNNLFYGEFNNRGPGADVSKRVHWIGF 310

Query: 312 HIIKNPQEALEYTVKTFINGTIWLPETGVPFR 343
           H++ N Q A ++T+  F+NG+ WLPETG+PFR
Sbjct: 311 HLL-NKQLANQFTIDNFVNGSYWLPETGIPFR 332

[Close]
BLAST of MS002436 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 2.1e-109
Identity = 198/334 (59.28%), Postives = 247/334 (73.95%), Query Frame = 0 

Query: 15  FFAVSLII---DFFMPVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGT 74
           FF VSL++    F       +FNATVS DGSG+F  +  AI+AAP+ S +RFYI +K G 
Sbjct: 8   FFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGI 67

Query: 75  YNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFEN 134
           Y EH+EV  QKTFI LIG++A+TT+IVDNRSNG+GF T  +AT  V+G NFMA+ +TFEN
Sbjct: 68  YKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFEN 127

Query: 135 SAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGL 194
           SA    GQA+A++ DQA +  YY+CVFL YQDTL+      FFK+C+IYG +DFIFG+GL
Sbjct: 128 SAEQKEGQAIAVM-DQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGL 187

Query: 195 AVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGR 254
            VFQDC IYAR     I +TAQSK A    SGF FQNC+V  SPE+ S K+ N+ ++LGR
Sbjct: 188 VVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKD-NVTMFLGR 247

Query: 255 PWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYH 314
           PWRDYS VIFM+SFLD VV PKGW  W P RP++LLFYAE+NNRGPGA+ S R++WPGYH
Sbjct: 248 PWRDYSMVIFMESFLDRVVQPKGWLEW-PGRPVNLLFYAEYNNRGPGADTSQRVDWPGYH 307

Query: 315 IIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
           ++ N  E  ++TV+ FINGT WLPETG+PFR  L
Sbjct: 308 VLHNAMEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]
BLAST of MS002436 vs. ExPASy TrEMBL
Match: A0A5D3BKF8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004210 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.5e-109
Identity = 202/335 (60.30%), Postives = 255/335 (76.12%), Query Frame = 0 

Query: 12  IACFFAVSLIIDFFMPVCSIVFNATVSLDGS-GDFKSIGAAISAAPDNSNSRFYIRIKSG 71
           + CFF    ++ F   + S  FNATVSLDGS   FK+I AAI+AAPDNSN+RFYIR+  G
Sbjct: 11  VMCFFN---LVPF---IHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYIRVTPG 70

Query: 72  TYNEHIEVESQKTFITLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFE 131
           TY+E +++   KTFI LIGDNA TT+IVD+RSN  GF TS SAT  V GNNF+AKS+TFE
Sbjct: 71  TYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAKSLTFE 130

Query: 132 NSAGPNNGQAVALLNDQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDG 191
           NSAGP NGQAVA+L DQA++  Y++C FLG+QDTL++ G   FFK+CEIYG+VDFIFG+G
Sbjct: 131 NSAGPQNGQAVAVL-DQAHFTAYHKCKFLGFQDTLYVKGTQ-FFKECEIYGSVDFIFGNG 190

Query: 192 LAVFQDCKIYARSSNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLG 251
           L +FQDC IYAR  N  IT+TAQSKE   + SGFSFQNC+V  S E+   K  N+++YLG
Sbjct: 191 LVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNK-ANVKIYLG 250

Query: 252 RPWRDYSTVIFMQSFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGY 311
           RPWR YS V+FM+SFLD  V P+GWS W P  P++ LFYAEFNNRGPGA++S R++W G+
Sbjct: 251 RPWRQYSKVVFMESFLDDEVVPEGWSEW-PGVPVNNLFYAEFNNRGPGADVSKRVHWTGF 310

Query: 312 HIIKNPQEALEYTVKTFINGTIWLPETGVPFRPEL 346
            ++ + Q A ++TV  F+NG+ WLPETG+PFR  L
Sbjct: 311 RVL-DKQSAYQFTVDNFVNGSKWLPETGIPFRDGL 334

[Close]
BLAST of MS002436 vs. ExPASy TrEMBL
Match: A0A6J1CR03 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013377 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 1.0e-108
Identity = 203/211 (96.21%), Postives = 208/211 (98.58%), Query Frame = 0 

Query: 31  IVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGD 90
           IVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGD
Sbjct: 3   IVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGD 62

Query: 91  NAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANY 150
           NAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANY
Sbjct: 63  NAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANY 122

Query: 151 ITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSSNNTITI 210
           IT+YRCVFLGYQDT+FINGNHLFFKDCEI GTVDFIFGDGLAVFQDCKIYARSSNNTIT+
Sbjct: 123 ITFYRCVFLGYQDTIFINGNHLFFKDCEICGTVDFIFGDGLAVFQDCKIYARSSNNTITV 182

Query: 211 TAQSKEAKHQNSGFSFQNCSVMASPELGSRK 242
           TAQSK++K Q SGFSFQNCSVMASPELGSRK
Sbjct: 183 TAQSKDSKDQKSGFSFQNCSVMASPELGSRK 213

[Close]
BLAST of MS002436 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 294.7 bits (753), Expect = 9.9e-80
Identity = 143/317 (45.11%), Postives = 206/317 (64.98%), Query Frame = 0 

Query: 27  PVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFIT 86
           PV    +N +V++DG+G+F +I  A+ AAP+ S +RF I IK G Y E++E+  +KT I 
Sbjct: 214 PVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 273

Query: 87  LIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLND 146
            IGD    TVI  NRS   G+ T Q+ T  V+G  ++AK I+F NSAGP   QAVA    
Sbjct: 274 FIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA-FRS 333

Query: 147 QANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSSN- 206
            +++  +YRC F GYQDTL+++    F+++C+IYGT+DFIFG+   VFQ+  +YAR  N 
Sbjct: 334 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 393

Query: 207 -NTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQS 266
            + I  TAQS+    Q +G S  NC ++A+P+L   KE N + YLGRPWR YS  + ++S
Sbjct: 394 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSRTVIIKS 453

Query: 267 FLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTV 326
           F+D +++P GW     D  L+ L+Y E+ N GPGAN++ R+ WPG+  I+N  EA ++TV
Sbjct: 454 FIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTV 513

Query: 327 KTFINGTIWLPETGVPF 342
             FI+G+ WL  TG+PF
Sbjct: 514 GPFIDGSTWLNSTGIPF 528

[Close]
BLAST of MS002436 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 288.9 bits (738), Expect = 5.4e-78
Identity = 145/311 (46.62%), Postives = 200/311 (64.31%), Query Frame = 0 

Query: 33  FNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNA 92
           ++ +V+LDG+G+F  I  AI  APD S++RF I IK G Y E++E++ +K  I ++GD  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGI 270

Query: 93  ATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYIT 152
             TVI  NRS   G+ T +SATFAV G  F+A+ ITF+N+AGP   QAVAL +D ++   
Sbjct: 271 DVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSD-SDLSV 330

Query: 153 YYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARSS--NNTITI 212
           ++RC   GYQDTL+ +    F+++C I GTVDFIFGDG  VFQ+C+I A+    N   TI
Sbjct: 331 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 390

Query: 213 TAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVV 272
           TAQ ++  +Q SGFS Q  ++ A  +L          YLGRPW+ YS  +F+++ +  VV
Sbjct: 391 TAQGRKDVNQPSGFSIQFSNISADADLVPYLN-TTRTYLGRPWKLYSRTVFIRNNMSDVV 450

Query: 273 NPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFING 332
            P+GW  W+ D  LD LFY EF N GPG+ +S R+ WPGYH+  N  +A  +TV  FI G
Sbjct: 451 RPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKG 510

Query: 333 TIWLPETGVPF 342
            +WLP TGV F
Sbjct: 511 NLWLPSTGVTF 519

[Close]
BLAST of MS002436 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 287.7 bits (735), Expect = 1.2e-77
Identity = 142/321 (44.24%), Postives = 205/321 (63.86%), Query Frame = 0 

Query: 27  PVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFIT 86
           PV     N  V+ +G+G++ +IG AISAAP++S +RF I IK G Y E+IE+  +KT I 
Sbjct: 200 PVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIM 259

Query: 87  LIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLND 146
            IGD    TVI  NRS   G+    SAT  VRG+ F+AK ++F N AGP   QAVA L  
Sbjct: 260 FIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVA-LRS 319

Query: 147 QANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYAR--SS 206
            ++   YYRC F  YQDT++++ +  F+++C+IYGTVDFIFGD   VFQ+C +YAR  + 
Sbjct: 320 SSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNP 379

Query: 207 NNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQS 266
           N  I  TAQ +E   + +G S  +  ++A+P+L    + N + YLGRPW+ YS  + M+S
Sbjct: 380 NQKIIYTAQGRENSREPTGISIISSRILAAPDL-IPVQANFKAYLGRPWQLYSRTVIMKS 439

Query: 267 FLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTV 326
           F+D +V+P GW  W  D  L+ L+Y E+ N GPG+N++ R+ WPG+  I+  +EA +++V
Sbjct: 440 FIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSV 499

Query: 327 KTFINGTIWLPETGVPFRPEL 346
             FI+G  WL  T +PF  +L
Sbjct: 500 GPFIDGNKWLNSTRIPFTLDL 518

[Close]
BLAST of MS002436 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 285.0 bits (728), Expect = 7.9e-77
Identity = 142/310 (45.81%), Postives = 201/310 (64.84%), Query Frame = 0 

Query: 34  NATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTYNEHIEVESQKTFITLIGDNAA 93
           N  V++DG G +++I  AI+ AP++S  R+ I +K G Y E+I+++ +KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 94  TTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLNDQANYITY 153
            T+I  +R+   G  T ++AT AV G  F+AK ITF N+AGP N QAVAL  D ++   +
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVD-SDQSAF 359

Query: 154 YRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYAR--SSNNTITIT 213
           YRC   GYQDTL+ +    F++DCEIYGT+DFIFG+G AV Q+CKIY R       +TIT
Sbjct: 360 YRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTIT 419

Query: 214 AQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQSFLDSVVN 273
           AQ +++ +QN+GF  QN  V+A+             YLGRPW+ YS  ++M +++  +V 
Sbjct: 420 AQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNTYMSQLVQ 479

Query: 274 PKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYTVKTFINGT 333
           P+GW  W  +  LD L+Y E+NN GPG   SGR+ WPGYHI+ + + AL +TV +FI+G 
Sbjct: 480 PRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM-DKRTALSFTVGSFIDGR 537

Query: 334 IWLPETGVPF 342
            WLP TGV F
Sbjct: 540 RWLPATGVTF 537

[Close]
BLAST of MS002436 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 282.3 bits (721), Expect = 5.1e-76
Identity = 142/318 (44.65%), Postives = 201/318 (63.21%), Query Frame = 0 

Query: 27  PVCSIVFNATVSLDGSGDFKSIGAAISAAPDNSNSRFYIRIKSGTY-NEHIEVESQKTFI 86
           P  +I  + TVS DGSG FK+I  AI  AP++S+ RF I +K+G Y  E+++V  +KT +
Sbjct: 266 PTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNL 325

Query: 87  TLIGDNAATTVIVDNRSNGTGFGTSQSATFAVRGNNFMAKSITFENSAGPNNGQAVALLN 146
             IGD    TVI   +S      T  +ATFA  G  F+ + +TFEN AGP   QAVA L 
Sbjct: 326 MFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVA-LR 385

Query: 147 DQANYITYYRCVFLGYQDTLFINGNHLFFKDCEIYGTVDFIFGDGLAVFQDCKIYARS-- 206
              ++   YRC  +GYQD L+++ N  FF++CEIYGTVDFIFG+   + Q C IYAR   
Sbjct: 386 VGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPM 445

Query: 207 SNNTITITAQSKEAKHQNSGFSFQNCSVMASPELGSRKEPNLEVYLGRPWRDYSTVIFMQ 266
           +   ITITAQ+++  +QN+G S   C ++A+P+L + K  +   YLGRPW+ YS V++M 
Sbjct: 446 AQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASK-GSYPTYLGRPWKLYSRVVYMM 505

Query: 267 SFLDSVVNPKGWSPWSPDRPLDLLFYAEFNNRGPGANISGRINWPGYHIIKNPQEALEYT 326
           S +   ++P+GW  W+    LD L+Y E+ N+G G+ I  R+ WPGYH+I +  EA ++T
Sbjct: 506 SDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFT 565

Query: 327 VKTFINGTIWLPETGVPF 342
           V  FI+G+ WLP TGV F
Sbjct: 566 VAQFISGSSWLPSTGVSF 581

The following BLAST results are available for this feature:
BLAST of MS002436 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6584281.15.6e-12567.36putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
KAG6584282.17.3e-12567.36putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
KAG7019877.11.9e-11768.73putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp... [more]
XP_022137308.15.2e-11563.24probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia][more]
XP_023511662.15.1e-11060.17probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo][more]
BLAST of MS002436 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O814151.4e-7845.11Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O048879.0e-7845.63Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q430622.6e-7745.37Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
Q9SMY77.7e-7746.62Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
O813011.7e-7644.24Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
BLAST of MS002436 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C9Z62.5e-11563.24Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1[more]
A0A5D3BHF73.2e-11060.24Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00420... [more]
A0A6J1DAG52.1e-10959.28Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1[more]
A0A5D3BKF83.5e-10960.30Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00421... [more]
A0A6J1CR031.0e-10896.21Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013377 PE=4 SV=1[more]
BLAST of MS002436 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G02300.19.9e-8045.11Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.15.4e-7846.62pectin methylesterase 44 [more]
AT4G02320.11.2e-7744.24Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05620.17.9e-7745.81Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.15.1e-7644.65pectin methylesterase PCR fragment F [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 34..330
e-value: 4.0E-109
score: 363.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 29..345
e-value: 1.5E-115
score: 387.9
NoneNo IPR availablePANTHERPTHR31707:SF205PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATEDcoord: 32..342
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 32..342
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 59..78
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 179..188
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 34..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002436.1MS002436.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0030599 pectinesterase activity