MS002403 (gene) Bitter gourd (TR) v1

Overview
NameMS002403
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalmodulin-binding receptor-like cytoplasmic kinase 3
Locationscaffold30: 4632370 .. 4636512 (-)
RNA-Seq ExpressionMS002403
SyntenyMS002403
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTCTTCTTGTTATTTCATCGATAGTGGCTTCCGTTATGGGCATGTTGTTGGTGCAATCGCCCAGAGTATTGGCTTCTGAGTTTATGATGGAATCGGGGGTTTCAACAAGTGTCTATCAGATTCCCTATCCAGTTTCTGATGGTTGTGAACGTTATTTCGTGAATGGGATCTCAATGGACAGAACTTTGTTCTGTAAGGTGCTGCAGTTTTTCCATGGATATCCTTGCATATTTGAGAATTCTGGGGGCAGTGATGGTTGCCATTGGGATAATTCATTCAGTAAGCACCCTATGATTTGGTTTTCATTTGGAAACTTCATCATGGAATTTTTAGTTCATATTTATCATCTTCAGATATATTCTTGCTCTATTTACATGGTATATCCGTAATAGAAGTTTAACTCTGCAACTTTTTTTGCAGCTGGTCTGTCGGTCAAAGCATCAAGGAAACTGTTAAAAGAAGAACAGAAGGAGGAATACCCTAACCTTAAAGACGGTCAGAATCATGATCGGATAGCAAAAGAGGATAAAAAATCTTTTGCATCCCCAAAAAATATTGGAATTGTTTTAACTGGAACCTTCGTTTTATGCTGTGGCGTCCTCTGTCCTTGTTTTTATAGAAAGAGGAGACGCACTGTTCAAAAAGCTTTAGTCAAAGAACAACAATCGGGTGAGTCATTGGTTCAGCCTGAACTTTTTTTGTGTGATTATTAGGAGTATTGCTACTAATTGATTCTTCGTCACCTATGACATATCAAAGCCATTAAAAGGTTAAATTGAGAGTGGTGGCTCAATTTTGAATTTTTTTTTTTTTCCATTTAGAATGGCCATAGCATAGCAGGGTTGAATATTTTAATTCTTTTATCTGAGACTTTTTGTTCACTTTTCATTTACTTGTGATTTGTACAAAACCTATGTTCATAGCTGTTAACTCATTCTTCTCCATATGTCATTGCATGCAGTATGTTAAAATTGTGTATTTTTTCTTTACTTTTATGAAGGCATTGCCCTTCTTTGACATTACCTGGTGAGAATCAGACGTGCTTGATAGTTGAGTTATGGATTCCTTTTTTTGCAGTGCATTCAAGCTCCATTGAAGTGATGAGCTCTGCTCCTGAAAAGATTCCACCAAGTCCACTTCGTGTACCACCTAGTCCTAGATATTCTCCATCTCCTAAACTAAAACGGCTTGGATCAGTTCATCTCAATATGAGTCAAGTTGTAAGAGCGACTCGTGATTTCTCACCATCTCTACGCATAGGTGAAGGAGGTTTTGGAACTGTCTACAAAGCTCAGCTGGAAGATGGGCACATAGTTGCCGTTAAACGTGCTAAAAAGGCAAGTACCAAATCTTTATGATGGTTTTATGTATTCCACTTTTACTAAACAGTGATGATAATGTGTTGTAAAATCTCAAATTCTATGTATAATGTTCGTACATGGACCCAATCCCCCATACAACCTCAGTTAGCGGGCACATGCCATCATGATATGTTAACCTTAAGTTTCAACATGCCTAATGCTCTGAAAAACACTAGAGCTGACAAGTATACTCCCAAGGTCCCAAACTAGACTACTTAGAGAAATTATTAATTCCCATAAAGGCATATAGTCATATCAGTCTTGTTGGACATAGTCACCTGCGATGCGACAATAAAGGTGTTCATTGGTTTGATTTGGTTGATTTTTGTTCAAATGGAAACTAAATCGATCTTCTCAATTTTTTAATAAACCCCTACTGTACAAAATTATAAAAAGTCGAAAACCAGTTTTGAATGATTGATTTAGTTTGGTTTTTTTCATTTTATATAGAATTTGAGGAAAAGTTAGATTTTGTGACATATAATATAATGGCCATGTTGGATGAAATTAGTAACGTCCAAGATTCTATAATGGAGTCAGATATAAAGGTTCCTTTATCCATTTTATTTTATTACCTCTGTAATCAGTAATAGTTCTTTGGGAACTTATATTGTAACCCGAACCCAATTATTTTGCAGGAAATCTTTGAGAATTCACGAACTGATTTCAGCAGTGAAGTAGAATTACTGGCCAAAATTGATCATCGTAGTCTGGTTAAGCTGTTAGGTTATGTTGACCATGGAAATGAACGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGATGGTAATGTTCTACCCGACGGAAGACTAGAAATTAAACTGCTGTTTAATTCTGATGACTCCATTGACGCTCATGACTGCTCTTCTTTTGTCCTTGTAGGTGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTATCTCCATCTATATGCAGGTTAGACACTCCTTTATTCGTTGGCTTATTCTGGTAACGTCAAAGTCATTTTTTGATATCAGGAAGGCTAATTTTGAAAGTCGATAAAATTACCTTCACATCATATCAATTTGATTGTTTGTGTGATTCTTTAATGTATAGAAGTGTTTGTTTAATTAGCAATTTGTATACTTCATTAAACTTCCTATTTGAGACTTATTTGGGAGAATTTATCCAAATTAGTATTTATTTTTTAAAACAGTTTTTAAGAGATGACTATAAAAGTTAAGATTATTTGGGCTTGCAATTACTATTCATAAATCTCACAGTAAGAACTACTCCAGCTGATATAGAAAATAAGTCATCCATAATTGAGAAAATAACTTCTAAAGACTGTTATTCAAATATGCTATTGTTGAACTTTTATCTAAAAAAATATAATATTGTTTCTAAAAACAGTTTCTAAACAGCCTTCACAATGTCATATTTTATTTGTTTTGAATTAGTAATTTCTTGAATATGATTAAATAACTATGAAATGTTATTCAATAGTTTATGATTCAATAATATTTTGTGCAACTCACTAGCAACTACTCAACTAGAAAAGTTGAGTTATAATTATTGTATCAATAAATTGAGTTTTATATGGAACTAAATCTTCCTGCTTAGATATCTCGCAGTATTTTTTCTGATGATGGTATTGGATCATTTTCTTTTTTCAGCCAGTTAACATTTATTTCTTGTTTACGATAGAGAAACAAATTATTCATAGAGATGTCAAATCGACCAACATTCTTCTTACTGAAAGCATGAGAGCGAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGCACATTGGGTACTGAACAAACGCACATTTCAACCCAGGTGAAAGGAACAGTTGGTTACCTAGACCCGGAGTATATGAAGACTTACCAACTTACTACTAAGAGTGATGTCTACTCATTCGGGATTTTACTCGTCGAAATTCTAACCGGACGGCGTCCGTTGGAAGCAAAGAGGCCTCCTGAGGAGCGGGTCACAATAAGATGGGTAAGTTGAACATTCTAATCATTTTCTTGGACACTTCTGTTAGGAATCATGGACGGTTGTAAGAAACTTGTTAAGTTTAAAAGAAGATGGATAATTGTTCTGTGTTTGCGATGAAACCTCCGAAGATGGATCCGAAAAATTAGAGCTTTTTCATCTAGAATCTGATGCTGCATCAACCTCTGATTTACTAATTTAGGTCTCTTTTAACTGACTAAATTCTGACACATACACTTGCAAGTTTAGAGTGTTCTTTACGTTCTAGGATGCAAATTTTACATCTGATCACATTCTGTATAAAAAGTTTATAAATTTACCAGATGGCATGTAAACTAATCGTTTATGAACCATCCTACCACTGTGTGTTATGGTTCTACAAATGATCCTTTTAATTCTTTTTGAAAGTAGGAACGTTACTCATTGTTTCAGAACGAGCAAGCCTTCATTTGTTTTGTCTGTTCACTAGATTTCATTTCATTAGTGAGTATGAGGGCCCAACCTCTCATGTTGCTCTTGGTTTACACATTGGTATTTGCAGGCTTTCACCAAGTATAGTGATGACAAAATACTGGAAACGTTAGATCCTTTGCTGGACGAAACTGTGGACGTGGAGATTGTCGTCAAAATGTTTGAGTTGGCAATTCAATGTGCAGCACCAGTACGAGCGGACCGTCCAGACATGAAGTTAGTTGGGGAGCAGTTGTGGGCAATAAGAGCAGATTACATATCACAGAAGAAGGATAAGGCAAGATTTCCA

mRNA sequence

ATGGCTTTTCTTCTTGTTATTTCATCGATAGTGGCTTCCGTTATGGGCATGTTGTTGGTGCAATCGCCCAGAGTATTGGCTTCTGAGTTTATGATGGAATCGGGGGTTTCAACAAGTGTCTATCAGATTCCCTATCCAGTTTCTGATGGTTGTGAACGTTATTTCGTGAATGGGATCTCAATGGACAGAACTTTGTTCTGTAAGGTGCTGCAGTTTTTCCATGGATATCCTTGCATATTTGAGAATTCTGGGGGCAGTGATGGTTGCCATTGGGATAATTCATTCACTGGTCTGTCGGTCAAAGCATCAAGGAAACTGTTAAAAGAAGAACAGAAGGAGGAATACCCTAACCTTAAAGACGGTCAGAATCATGATCGGATAGCAAAAGAGGATAAAAAATCTTTTGCATCCCCAAAAAATATTGGAATTGTTTTAACTGGAACCTTCGTTTTATGCTGTGGCGTCCTCTGTCCTTGTTTTTATAGAAAGAGGAGACGCACTGTTCAAAAAGCTTTAGTCAAAGAACAACAATCGGTGCATTCAAGCTCCATTGAAGTGATGAGCTCTGCTCCTGAAAAGATTCCACCAAGTCCACTTCGTGTACCACCTAGTCCTAGATATTCTCCATCTCCTAAACTAAAACGGCTTGGATCAGTTCATCTCAATATGAGTCAAGTTGTAAGAGCGACTCGTGATTTCTCACCATCTCTACGCATAGGTGAAGGAGGTTTTGGAACTGTCTACAAAGCTCAGCTGGAAGATGGGCACATAGTTGCCGTTAAACGTGCTAAAAAGCAAGAAATCTTTGAGAATTCACGAACTGATTTCAGCAGTGAAGTAGAATTACTGGCCAAAATTGATCATCGTAGTCTGGTTAAGCTGTTAGGTTATGTTGACCATGGAAATGAACGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGATGGTGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTATCTCCATCTATATGCAGAGAAACAAATTATTCATAGAGATGTCAAATCGACCAACATTCTTCTTACTGAAAGCATGAGAGCGAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGCACATTGGGTACTGAACAAACGCACATTTCAACCCAGGTGAAAGGAACAGTTGGTTACCTAGACCCGGAGTATATGAAGACTTACCAACTTACTACTAAGAGTGATGTCTACTCATTCGGGATTTTACTCGTCGAAATTCTAACCGGACGGCGTCCGTTGGAAGCAAAGAGGCCTCCTGAGGAGCGGGTCACAATAAGATGGGCTTTCACCAAGTATAGTGATGACAAAATACTGGAAACGTTAGATCCTTTGCTGGACGAAACTGTGGACGTGGAGATTGTCGTCAAAATGTTTGAGTTGGCAATTCAATGTGCAGCACCAGTACGAGCGGACCGTCCAGACATGAAGTTAGTTGGGGAGCAGTTGTGGGCAATAAGAGCAGATTACATATCACAGAAGAAGGATAAGGCAAGATTTCCA

Coding sequence (CDS)

ATGGCTTTTCTTCTTGTTATTTCATCGATAGTGGCTTCCGTTATGGGCATGTTGTTGGTGCAATCGCCCAGAGTATTGGCTTCTGAGTTTATGATGGAATCGGGGGTTTCAACAAGTGTCTATCAGATTCCCTATCCAGTTTCTGATGGTTGTGAACGTTATTTCGTGAATGGGATCTCAATGGACAGAACTTTGTTCTGTAAGGTGCTGCAGTTTTTCCATGGATATCCTTGCATATTTGAGAATTCTGGGGGCAGTGATGGTTGCCATTGGGATAATTCATTCACTGGTCTGTCGGTCAAAGCATCAAGGAAACTGTTAAAAGAAGAACAGAAGGAGGAATACCCTAACCTTAAAGACGGTCAGAATCATGATCGGATAGCAAAAGAGGATAAAAAATCTTTTGCATCCCCAAAAAATATTGGAATTGTTTTAACTGGAACCTTCGTTTTATGCTGTGGCGTCCTCTGTCCTTGTTTTTATAGAAAGAGGAGACGCACTGTTCAAAAAGCTTTAGTCAAAGAACAACAATCGGTGCATTCAAGCTCCATTGAAGTGATGAGCTCTGCTCCTGAAAAGATTCCACCAAGTCCACTTCGTGTACCACCTAGTCCTAGATATTCTCCATCTCCTAAACTAAAACGGCTTGGATCAGTTCATCTCAATATGAGTCAAGTTGTAAGAGCGACTCGTGATTTCTCACCATCTCTACGCATAGGTGAAGGAGGTTTTGGAACTGTCTACAAAGCTCAGCTGGAAGATGGGCACATAGTTGCCGTTAAACGTGCTAAAAAGCAAGAAATCTTTGAGAATTCACGAACTGATTTCAGCAGTGAAGTAGAATTACTGGCCAAAATTGATCATCGTAGTCTGGTTAAGCTGTTAGGTTATGTTGACCATGGAAATGAACGCATTATCATTACGGAATATGTAGGAAATGGTACTCTTAGAGAACACTTGGATGGTGTGCATGGAAAAGTATTGGATTTTAATCAGAGACTTGAAATTGCCATTGACATTGCTCATGGACTAACTTATCTCCATCTATATGCAGAGAAACAAATTATTCATAGAGATGTCAAATCGACCAACATTCTTCTTACTGAAAGCATGAGAGCGAAGGTGGCGGATTTTGGATTTGCAAGGCTTGGCACATTGGGTACTGAACAAACGCACATTTCAACCCAGGTGAAAGGAACAGTTGGTTACCTAGACCCGGAGTATATGAAGACTTACCAACTTACTACTAAGAGTGATGTCTACTCATTCGGGATTTTACTCGTCGAAATTCTAACCGGACGGCGTCCGTTGGAAGCAAAGAGGCCTCCTGAGGAGCGGGTCACAATAAGATGGGCTTTCACCAAGTATAGTGATGACAAAATACTGGAAACGTTAGATCCTTTGCTGGACGAAACTGTGGACGTGGAGATTGTCGTCAAAATGTTTGAGTTGGCAATTCAATGTGCAGCACCAGTACGAGCGGACCGTCCAGACATGAAGTTAGTTGGGGAGCAGTTGTGGGCAATAAGAGCAGATTACATATCACAGAAGAAGGATAAGGCAAGATTTCCA

Protein sequence

MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGISMDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKDGQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVHSSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP
Homology
BLAST of MS002403 vs. NCBI nr
Match: XP_022135089.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Momordica charantia])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.2e-297
Identity = 523/524 (99.81%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS
Sbjct: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD
Sbjct: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH
Sbjct: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180

Query: 181 SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG 240
           SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG
Sbjct: 181 SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG 240

Query: 241 EGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH 300
           EGGFGTVYKAQLEDGHIVAVKRAKK EIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH
Sbjct: 241 EGGFGTVYKAQLEDGHIVAVKRAKK-EIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH 300

Query: 301 GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV 360
           GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV
Sbjct: 301 GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV 360

Query: 361 KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV 420
           KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV
Sbjct: 361 KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV 420

Query: 421 YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK 480
           YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK
Sbjct: 421 YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK 480

Query: 481 MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP
Sbjct: 481 MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 523

BLAST of MS002403 vs. NCBI nr
Match: XP_022960815.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata] >XP_022960816.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata])

HSP 1 Score: 880.2 bits (2273), Expect = 9.0e-252
Identity = 443/525 (84.38%), Postives = 489/525 (93.14%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLV+S +VAS+  +LLVQSP V ASEFMMESGVS S  QIPY VSDGCERYF+NGI 
Sbjct: 1   MAFLLVVSCVVASITAVLLVQSPLVFASEFMMESGVS-SANQIPYSVSDGCERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+++FCK+LQF HGYP IFE+S GSDGC WD+SF+G+ VKASRKLLKEE+KEE  N+KD
Sbjct: 61  MDKSMFCKLLQFLHGYPRIFEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANVKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           G+   R AKED KSFA+P+NIGIV+TGTFVLCCGVLCPCFY+KRRRTVQ+AL KEQQSVH
Sbjct: 121 GKYRHRKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IEVM+SAPEKI  SPLRVPPSPRYSPSPK KRLGSV LNMSQV +AT+DFSP+LRI
Sbjct: 181 LASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKA+LEDGHIVA+KRAKK E+FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKARLEDGHIVAIKRAKK-ELFENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKS+NILLTE+MRAKVADFGFARLGTLG+EQTHISTQVKGTVGYLDP+YMKTYQLT KSD
Sbjct: 361 VKSSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE K+PPEERVTI+WAF KYS+DKILETLDPL++ET++ +IV+
Sbjct: 421 VYSFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEADIVL 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRA+YISQKK+KAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARLP 523

BLAST of MS002403 vs. NCBI nr
Match: KAG6589818.1 (Calmodulin-binding receptor-like cytoplasmic kinase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 876.3 bits (2263), Expect = 1.3e-250
Identity = 441/525 (84.00%), Postives = 486/525 (92.57%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLV+S IVAS+  +LLVQSP V ASEFMMESGVS S  QIPY VSDGCERYF+NGI 
Sbjct: 1   MAFLLVVSCIVASITAVLLVQSPLVFASEFMMESGVS-SANQIPYSVSDGCERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+++FCK+LQF  GYPCIFE+S GSDGC WD+SF+G+ V+ASRK LKEE+KEE  N+KD
Sbjct: 61  MDKSMFCKLLQFLRGYPCIFEDSWGSDGCRWDSSFSGMRVEASRKSLKEERKEEDANVKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQ   R  KED KSFA+P+NIGIV+TGTFVLCCGVLCPCFY+KRRRTVQ+AL KEQQSVH
Sbjct: 121 GQYRHRKEKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IEVM+SAPEKI  SPLRVPPSPRYSPSPK KRLGSV LNMSQV +AT+DFSP+LRI
Sbjct: 181 LASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKA+LEDGHIVA+KRAKK E+FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKARLEDGHIVAIKRAKK-ELFENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKS+NILLTE+MRAKV DFGFARLGTLG+EQTHISTQVKGTVGYLDP+YMKTYQLT KSD
Sbjct: 361 VKSSNILLTETMRAKVGDFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE K+PPEERVTI+WAF KYS+DKILETLDPL++ET++ +IV+
Sbjct: 421 VYSFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEADIVL 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRA+YISQKK+KAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARLP 523

BLAST of MS002403 vs. NCBI nr
Match: XP_038879737.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Benincasa hispida])

HSP 1 Score: 874.0 bits (2257), Expect = 6.5e-250
Identity = 446/525 (84.95%), Postives = 479/525 (91.24%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLV+SSI+AS+  +LL+QSP V ASEFMMESGVS    QIPY VS GCERYF+NGI 
Sbjct: 1   MAFLLVMSSIIASITAVLLMQSPGVFASEFMMESGVSRG-NQIPYSVSKGCERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+ +FCK+LQF  GYP IFENS GSD C WDNSF+G+ VK SRKLLKEE+K+EY NLKD
Sbjct: 61  MDKIVFCKLLQFLRGYPGIFENSRGSDDCCWDNSFSGIRVKTSRKLLKEERKDEYINLKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQ   + AKED KSFA+ KNIGI +TGTFVLCCGVLCPCFYRKRRR+VQK    EQQSVH
Sbjct: 121 GQYLHQRAKEDPKSFATSKNIGIAVTGTFVLCCGVLCPCFYRKRRRSVQKLSEIEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IE M+S PEKI  SPLRVPPSPRYSPSPK KRLGSVHLNMSQV RAT++FSP+LRI
Sbjct: 181 LASTIEAMNSTPEKILASPLRVPPSPRYSPSPKFKRLGSVHLNMSQVARATQEFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKAQLEDG IVA+KRAKK++ FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKAQLEDGQIVAIKRAKKEQ-FENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD
Sbjct: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE KRPP+ERVTIRWAF KYS+DKI+ETLDPL++ETVD +IVV
Sbjct: 421 VYSFGILLVEILTGRRPLEVKRPPDERVTIRWAFNKYSEDKIVETLDPLMEETVDADIVV 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARVP 523

BLAST of MS002403 vs. NCBI nr
Match: XP_022987686.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima] >XP_022987687.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima] >XP_022987688.1 calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima])

HSP 1 Score: 870.9 bits (2249), Expect = 5.5e-249
Identity = 441/525 (84.00%), Postives = 485/525 (92.38%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           M+FLLV+S IVAS+  ++LVQSP V ASEFMMESGVS S  QIPY VSDG ERYF+NGI 
Sbjct: 1   MSFLLVVSCIVASITAVMLVQSPLVFASEFMMESGVS-SANQIPYSVSDGFERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+++FCK+LQF HGYP I E+S GSDGC WD+SF+G+ VKASRKLLKEE+KEE  N KD
Sbjct: 61  MDKSMFCKLLQFLHGYPRILEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANFKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQ     AKED KSFA+P+NIGIV+TGTFVLCCGVLCPCFY+KRRRTVQ+AL KEQQSVH
Sbjct: 121 GQYRHWKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IEVM+SAPEKI  SPLRVPPSPRYSPSPK KRLGSV LNMSQV +AT+DFSP+LRI
Sbjct: 181 LASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKA+LEDGHIVA+KRAKK E+FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKARLEDGHIVAIKRAKK-ELFENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKS+NILLTE+MRAKVADFGFARLGTLG+EQTHISTQVKGTVGYLDP+YMKTYQLT KSD
Sbjct: 361 VKSSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE K+PPEERVTI+WAF KYS+DKILETLDPL++ET++ EIV+
Sbjct: 421 VYSFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEAEIVL 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRA+YISQKK+KAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARVP 523

BLAST of MS002403 vs. ExPASy Swiss-Prot
Match: Q9ASQ5 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRCK3 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.5e-143
Identity = 276/522 (52.87%), Postives = 353/522 (67.62%), Query Frame = 0

Query: 10  IVASVMGML-LVQSPRVLASEFMMESGVSTS---VYQIPYPVSDGCERYFVNGISMDRTL 69
           ++ ++ G+L L+Q     AS   + S V  S    Y  PY        + +NG  +++  
Sbjct: 12  VITALFGLLMLLQIKETSASTSFVSSSVCKSDHLTYTKPYQQG---SLFTINGNPVEKLR 71

Query: 70  FCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKDGQNHD 129
           FC+ L+F     CIFE+S   D C        +     R+ L+E+       +KD +N  
Sbjct: 72  FCEALRFHKANGCIFEDSFSDDFC-------TIHSLLGRRFLEEK------TVKDSKN-- 131

Query: 130 RIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVHSSSIE 189
                  K+  S   + I  +G  +LCC + CPCF+++R+    + L KE  SVH  S  
Sbjct: 132 ----SKPKTEYSHVKVSIAGSGFLLLCCALCCPCFHKERKANSHEVLPKESNSVHQVSSF 191

Query: 190 VMSSAPEKIPPSPLRVPPSP--------RYSPSPKLKRLGSVHLNMSQVVRATRDFSPSL 249
            MS + EKIP SP R PPSP        RY+ SP+  RLG ++L MSQ+  AT +F+ S 
Sbjct: 192 EMSPSSEKIPQSPFRAPPSPSRVPQSPSRYAMSPRPSRLGPLNLTMSQINTATGNFADSH 251

Query: 250 RIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGY 309
           +IGEGGFG V+K  L+DG +VA+KRAKK E FEN RT+F SEV+LL+KI HR+LVKLLGY
Sbjct: 252 QIGEGGFGVVFKGVLDDGQVVAIKRAKK-EHFENLRTEFKSEVDLLSKIGHRNLVKLLGY 311

Query: 310 VDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIH 369
           VD G+ER+IITEYV NGTLR+HLDG  G  L+FNQRLEI ID+ HGLTYLH YAE+QIIH
Sbjct: 312 VDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEIVIDVCHGLTYLHSYAERQIIH 371

Query: 370 RDVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTK 429
           RD+KS+NILLT+SMRAKVADFGFAR G   + QTHI TQVKGTVGYLDPEYMKTY LT K
Sbjct: 372 RDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQVKGTVGYLDPEYMKTYHLTAK 431

Query: 430 SDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEI 489
           SDVYSFGILLVEILTGRRP+EAKR P+ER+T+RWAF KY++ ++ E +DP   E VD +I
Sbjct: 432 SDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKYNEGRVFELVDPNARERVDEKI 491

Query: 490 VVKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKD 520
           + KMF LA QCAAP + +RPDM+ VG+QLWAIR+ Y+ +  +
Sbjct: 492 LRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSYLRRSME 510

BLAST of MS002403 vs. ExPASy Swiss-Prot
Match: Q9FIL7 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRCK1 PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.3e-93
Identity = 165/306 (53.92%), Postives = 224/306 (73.20%), Query Frame = 0

Query: 208 SPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQE 267
           S S K    G V  +  ++ RAT +FS   +IGEGGFGTV+K +L+DG IVA+KRA+K  
Sbjct: 122 SSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDDGTIVAIKRARKNN 181

Query: 268 IFENSRTDFSSEVELLAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKV 327
             ++   +F +E+  L+KI+H +LVKL G+++HG+E++I+ EYV NG LREHLDG+ G  
Sbjct: 182 YGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGLRGNR 241

Query: 328 LDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLG 387
           L+  +RLEIAID+AH LTYLH Y +  IIHRD+K++NIL+T  +RAKVADFGFARL +  
Sbjct: 242 LEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARLVSED 301

Query: 388 TEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERV 447
              THISTQVKG+ GY+DP+Y++T+QLT KSDVYSFG+LLVEILTGRRP+E KRP ++R+
Sbjct: 302 LGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPRKDRL 361

Query: 448 TIRWAFTKYSDDKILETLDPLLDET-VDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQL 507
           T++WA  +  DD+ +  +DP L      +E+  KM  LA +C  P RA RP MK + E+L
Sbjct: 362 TVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGIAEKL 421

Query: 508 WAIRAD 513
           WAIR +
Sbjct: 422 WAIRRE 427

BLAST of MS002403 vs. ExPASy Swiss-Prot
Match: Q8VZJ9 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CRCK2 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.1e-90
Identity = 166/293 (56.66%), Postives = 211/293 (72.01%), Query Frame = 0

Query: 225 QVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKK--QEIFENSRTDFSSEVEL 284
           ++  AT++FSPS RIG+GGFGTVYK +L DG   AVKRAKK   +  + +  +F SE++ 
Sbjct: 111 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 170

Query: 285 LAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAH 344
           LA++ H SLVK  G+V H +E+I++ EYV NGTLR+HLD   GK LD   RL+IA D+AH
Sbjct: 171 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 230

Query: 345 GLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLG-TLGTEQTHISTQVKGTV 404
            +TYLH+Y +  IIHRD+KS+NILLTE+ RAKVADFGFARL     +  TH+STQVKGT 
Sbjct: 231 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 290

Query: 405 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKI 464
           GYLDPEY+ TYQLT KSDVYSFG+LLVE+LTGRRP+E  R  +ER+TIRWA  K++    
Sbjct: 291 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 350

Query: 465 LETLDPLLDETVDVEIVV-KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADY 514
           +  LDP L++     + + K+ E+A QC AP R  RP MK   E LW IR DY
Sbjct: 351 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDY 403

BLAST of MS002403 vs. ExPASy Swiss-Prot
Match: Q9LK35 (Receptor-like protein kinase THESEUS 1 OS=Arabidopsis thaliana OX=3702 GN=THE1 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.4e-61
Identity = 135/296 (45.61%), Postives = 174/296 (58.78%), Query Frame = 0

Query: 216 LGSVHLN----MSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFEN 275
           L S HL       +++ AT  F  S  +G GGFG VYK  LEDG  VAVKR   +   E 
Sbjct: 489 LASTHLGRCFMFQEIMDATNKFDESSLLGVGGFGRVYKGTLEDGTKVAVKRGNPRS--EQ 548

Query: 276 SRTDFSSEVELLAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFN 335
              +F +E+E+L+K+ HR LV L+GY D  +E I++ EY+ NG LR HL G     L + 
Sbjct: 549 GMAEFRTEIEMLSKLRHRHLVSLIGYCDERSEMILVYEYMANGPLRSHLYGADLPPLSWK 608

Query: 336 QRLEIAIDIAHGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLGTEQT 395
           QRLEI I  A GL YLH  A + IIHRDVK+TNILL E++ AKVADFG ++ G    +QT
Sbjct: 609 QRLEICIGAARGLHYLHTGASQSIIHRDVKTTNILLDENLVAKVADFGLSKTGP-SLDQT 668

Query: 396 HISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRW 455
           H+ST VKG+ GYLDPEY +  QLT KSDVYSFG++L+E+L  R  L    P E+     W
Sbjct: 669 HVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLMEVLCCRPALNPVLPREQVNIAEW 728

Query: 456 AFTKYSDDKILETLDPLLDETVDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQLW 508
           A        + + +D  L   V+   + K  E A +C A    DRP M   G+ LW
Sbjct: 729 AMAWQKKGLLDQIMDSNLTGKVNPASLKKFGETAEKCLAEYGVDRPSM---GDVLW 778

BLAST of MS002403 vs. ExPASy Swiss-Prot
Match: O80623 (Probable receptor-like protein kinase At2g39360 OS=Arabidopsis thaliana OX=3702 GN=At2g39360 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 5.8e-60
Identity = 132/299 (44.15%), Postives = 178/299 (59.53%), Query Frame = 0

Query: 223 MSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVEL 282
           ++ +  AT DF  SL IG GGFG VYK  L D   VAVKR   Q        +F +EVE+
Sbjct: 477 LALIKEATDDFDESLVIGVGGFGKVYKGVLRDKTEVAVKRGAPQS--RQGLAEFKTEVEM 536

Query: 283 LAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKV-LDFNQRLEIAIDIA 342
           L +  HR LV L+GY D  +E II+ EY+  GTL++HL  +  K  L + QRLEI +  A
Sbjct: 537 LTQFRHRHLVSLIGYCDENSEMIIVYEYMEKGTLKDHLYDLDDKPRLSWRQRLEICVGAA 596

Query: 343 HGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTV 402
            GL YLH  + + IIHRDVKS NILL ++  AKVADFG ++ G    +QTH+ST VKG+ 
Sbjct: 597 RGLHYLHTGSTRAIIHRDVKSANILLDDNFMAKVADFGLSKTGP-DLDQTHVSTAVKGSF 656

Query: 403 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKI 462
           GYLDPEY+   QLT KSDVYSFG++++E++ GR  ++   P E+   I WA       K+
Sbjct: 657 GYLDPEYLTRQQLTEKSDVYSFGVVMLEVVCGRPVIDPSLPREKVNLIEWAMKLVKKGKL 716

Query: 463 LETLDPLLDETVDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDK 521
            + +DP L   V +E V K  E+  +C +    +RP M   G+ LW +      Q KD+
Sbjct: 717 EDIIDPFLVGKVKLEEVKKYCEVTEKCLSQNGIERPAM---GDLLWNLEFMLQVQAKDE 769

BLAST of MS002403 vs. ExPASy TrEMBL
Match: A0A6J1C0H6 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Momordica charantia OX=3673 GN=LOC111007158 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.5e-297
Identity = 523/524 (99.81%), Postives = 523/524 (99.81%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS
Sbjct: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD
Sbjct: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH
Sbjct: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180

Query: 181 SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG 240
           SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG
Sbjct: 181 SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIG 240

Query: 241 EGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH 300
           EGGFGTVYKAQLEDGHIVAVKRAKK EIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH
Sbjct: 241 EGGFGTVYKAQLEDGHIVAVKRAKK-EIFENSRTDFSSEVELLAKIDHRSLVKLLGYVDH 300

Query: 301 GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV 360
           GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV
Sbjct: 301 GNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDV 360

Query: 361 KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV 420
           KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV
Sbjct: 361 KSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDV 420

Query: 421 YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK 480
           YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK
Sbjct: 421 YSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVVK 480

Query: 481 MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP
Sbjct: 481 MFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 523

BLAST of MS002403 vs. ExPASy TrEMBL
Match: A0A6J1H8N3 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111461507 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.4e-252
Identity = 443/525 (84.38%), Postives = 489/525 (93.14%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           MAFLLV+S +VAS+  +LLVQSP V ASEFMMESGVS S  QIPY VSDGCERYF+NGI 
Sbjct: 1   MAFLLVVSCVVASITAVLLVQSPLVFASEFMMESGVS-SANQIPYSVSDGCERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+++FCK+LQF HGYP IFE+S GSDGC WD+SF+G+ VKASRKLLKEE+KEE  N+KD
Sbjct: 61  MDKSMFCKLLQFLHGYPRIFEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANVKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           G+   R AKED KSFA+P+NIGIV+TGTFVLCCGVLCPCFY+KRRRTVQ+AL KEQQSVH
Sbjct: 121 GKYRHRKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IEVM+SAPEKI  SPLRVPPSPRYSPSPK KRLGSV LNMSQV +AT+DFSP+LRI
Sbjct: 181 LASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKA+LEDGHIVA+KRAKK E+FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKARLEDGHIVAIKRAKK-ELFENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKS+NILLTE+MRAKVADFGFARLGTLG+EQTHISTQVKGTVGYLDP+YMKTYQLT KSD
Sbjct: 361 VKSSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE K+PPEERVTI+WAF KYS+DKILETLDPL++ET++ +IV+
Sbjct: 421 VYSFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEADIVL 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRA+YISQKK+KAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARLP 523

BLAST of MS002403 vs. ExPASy TrEMBL
Match: A0A6J1JK63 (calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111485167 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.6e-249
Identity = 441/525 (84.00%), Postives = 485/525 (92.38%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCERYFVNGIS 60
           M+FLLV+S IVAS+  ++LVQSP V ASEFMMESGVS S  QIPY VSDG ERYF+NGI 
Sbjct: 1   MSFLLVVSCIVASITAVMLVQSPLVFASEFMMESGVS-SANQIPYSVSDGFERYFMNGIP 60

Query: 61  MDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKD 120
           MD+++FCK+LQF HGYP I E+S GSDGC WD+SF+G+ VKASRKLLKEE+KEE  N KD
Sbjct: 61  MDKSMFCKLLQFLHGYPRILEDSWGSDGCRWDSSFSGMRVKASRKLLKEERKEEDANFKD 120

Query: 121 GQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVH 180
           GQ     AKED KSFA+P+NIGIV+TGTFVLCCGVLCPCFY+KRRRTVQ+AL KEQQSVH
Sbjct: 121 GQYRHWKAKEDPKSFATPQNIGIVVTGTFVLCCGVLCPCFYQKRRRTVQRALDKEQQSVH 180

Query: 181 -SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLRI 240
            +S+IEVM+SAPEKI  SPLRVPPSPRYSPSPK KRLGSV LNMSQV +AT+DFSP+LRI
Sbjct: 181 LASTIEVMNSAPEKILASPLRVPPSPRYSPSPKFKRLGSVRLNMSQVAKATQDFSPALRI 240

Query: 241 GEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYVD 300
           GEGGFGTVYKA+LEDGHIVA+KRAKK E+FENSRTDF SEVELL+KIDHRSLVKLLGYVD
Sbjct: 241 GEGGFGTVYKARLEDGHIVAIKRAKK-ELFENSRTDFGSEVELLSKIDHRSLVKLLGYVD 300

Query: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360
           HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD
Sbjct: 301 HGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRD 360

Query: 361 VKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKSD 420
           VKS+NILLTE+MRAKVADFGFARLGTLG+EQTHISTQVKGTVGYLDP+YMKTYQLT KSD
Sbjct: 361 VKSSNILLTETMRAKVADFGFARLGTLGSEQTHISTQVKGTVGYLDPDYMKTYQLTPKSD 420

Query: 421 VYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIVV 480
           VYSFGILLVEILTGRRPLE K+PPEERVTI+WAF KYS+DKILETLDPL++ET++ EIV+
Sbjct: 421 VYSFGILLVEILTGRRPLEVKKPPEERVTIKWAFNKYSEDKILETLDPLMEETLEAEIVL 480

Query: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           KMFELAIQCAAPVRADRPDMKLVGEQLWAIRA+YISQKK+KAR P
Sbjct: 481 KMFELAIQCAAPVRADRPDMKLVGEQLWAIRAEYISQKKEKARVP 523

BLAST of MS002403 vs. ExPASy TrEMBL
Match: A0A5D3C366 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00270 PE=4 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.3e-244
Identity = 442/526 (84.03%), Postives = 478/526 (90.87%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCER-YFVNGI 60
           MAFLLV+SSI+AS+  +LLVQS  V ASEFMMESG S    QIP  VS GC+  YF+NGI
Sbjct: 1   MAFLLVMSSIMASITAVLLVQSSGVFASEFMMESGGSRG-NQIPCSVSKGCDHCYFMNGI 60

Query: 61  SMDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLK 120
            MD+ +FCK+LQF  GYPCIFENS GSD   WDNSF+G+ VK SRKLLK E+KEE+ NLK
Sbjct: 61  PMDKIVFCKLLQFLRGYPCIFENSRGSDDGRWDNSFSGIRVKVSRKLLK-ERKEEHINLK 120

Query: 121 DGQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSV 180
           DGQ   + AKED KSFA+P+NIGI +TGTF+LCCGVLCPCFYRKRRR+VQK L +EQQSV
Sbjct: 121 DGQYLPQRAKEDPKSFATPRNIGIAVTGTFILCCGVLCPCFYRKRRRSVQK-LTEEQQSV 180

Query: 181 H-SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLR 240
           H +S+IE M+SA EKI  SPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRAT++FSP+LR
Sbjct: 181 HLASTIEAMNSAHEKILASPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATQEFSPALR 240

Query: 241 IGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYV 300
           IGEGGFGTVYKA+LEDG IVA+KRAKK++ FENSRTDF SEVELL+KIDHRSLVKLLGYV
Sbjct: 241 IGEGGFGTVYKAKLEDGQIVAIKRAKKEQ-FENSRTDFGSEVELLSKIDHRSLVKLLGYV 300

Query: 301 DHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR 360
           DHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR
Sbjct: 301 DHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR 360

Query: 361 DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS 420
           DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS
Sbjct: 361 DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS 420

Query: 421 DVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIV 480
           DVYSFGILLVEILTGRRPLE KRPPEERVTIRWAF KYS+DK LETLDPL++ETVD +IV
Sbjct: 421 DVYSFGILLVEILTGRRPLEVKRPPEERVTIRWAFNKYSEDKFLETLDPLMEETVDADIV 480

Query: 481 VKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           +KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKAR P
Sbjct: 481 IKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARLP 522

BLAST of MS002403 vs. ExPASy TrEMBL
Match: A0A5A7U915 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00900 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.7e-244
Identity = 441/526 (83.84%), Postives = 478/526 (90.87%), Query Frame = 0

Query: 1   MAFLLVISSIVASVMGMLLVQSPRVLASEFMMESGVSTSVYQIPYPVSDGCER-YFVNGI 60
           MAFLLV+SSI+AS+  +LLVQS  V ASEFMMESG S    QIP  VS GC+  YF+NGI
Sbjct: 1   MAFLLVMSSIMASITAVLLVQSSGVFASEFMMESGGSRG-NQIPCSVSKGCDHCYFMNGI 60

Query: 61  SMDRTLFCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLK 120
            MD+ +FCK+LQF  GYPCIFENS GSD   WDNSF+G+ VK SRKLLK E+KEE+ NLK
Sbjct: 61  PMDKIVFCKLLQFLRGYPCIFENSRGSDDGRWDNSFSGIRVKVSRKLLK-ERKEEHINLK 120

Query: 121 DGQNHDRIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSV 180
           DGQ   + AKED KSFA+P+NIGI +TGTF+LCCGVLCPCFYRKRRR+VQK L +EQQSV
Sbjct: 121 DGQYLPQRAKEDPKSFATPRNIGIAVTGTFILCCGVLCPCFYRKRRRSVQK-LTEEQQSV 180

Query: 181 H-SSSIEVMSSAPEKIPPSPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATRDFSPSLR 240
           H +S+IE M+SA EKI  SPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRAT++FSP+LR
Sbjct: 181 HLASTIEAMNSAHEKILASPLRVPPSPRYSPSPKLKRLGSVHLNMSQVVRATQEFSPALR 240

Query: 241 IGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGYV 300
           IGEGGFGTVYKA+LEDG IVA+KRAKK++ FENSRTDF SEVELL+KIDHRSLVKLLGYV
Sbjct: 241 IGEGGFGTVYKAKLEDGQIVAIKRAKKEQ-FENSRTDFGSEVELLSKIDHRSLVKLLGYV 300

Query: 301 DHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR 360
           DHGNER+IITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR
Sbjct: 301 DHGNERLIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIHR 360

Query: 361 DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS 420
           DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS
Sbjct: 361 DVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTKS 420

Query: 421 DVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEIV 480
           DVYSFGILLVEILTGRRPLE KRPPEERVTIRWAF KYS+DK LETLDPL++ETVD +IV
Sbjct: 421 DVYSFGILLVEILTGRRPLEVKRPPEERVTIRWAFNKYSEDKFLETLDPLMEETVDADIV 480

Query: 481 VKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARFP 525
           +KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKAR P
Sbjct: 481 IKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDKARLP 522

BLAST of MS002403 vs. TAIR 10
Match: AT2G11520.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 )

HSP 1 Score: 509.2 bits (1310), Expect = 3.9e-144
Identity = 276/522 (52.87%), Postives = 353/522 (67.62%), Query Frame = 0

Query: 10  IVASVMGML-LVQSPRVLASEFMMESGVSTS---VYQIPYPVSDGCERYFVNGISMDRTL 69
           ++ ++ G+L L+Q     AS   + S V  S    Y  PY        + +NG  +++  
Sbjct: 12  VITALFGLLMLLQIKETSASTSFVSSSVCKSDHLTYTKPYQQG---SLFTINGNPVEKLR 71

Query: 70  FCKVLQFFHGYPCIFENSGGSDGCHWDNSFTGLSVKASRKLLKEEQKEEYPNLKDGQNHD 129
           FC+ L+F     CIFE+S   D C        +     R+ L+E+       +KD +N  
Sbjct: 72  FCEALRFHKANGCIFEDSFSDDFC-------TIHSLLGRRFLEEK------TVKDSKN-- 131

Query: 130 RIAKEDKKSFASPKNIGIVLTGTFVLCCGVLCPCFYRKRRRTVQKALVKEQQSVHSSSIE 189
                  K+  S   + I  +G  +LCC + CPCF+++R+    + L KE  SVH  S  
Sbjct: 132 ----SKPKTEYSHVKVSIAGSGFLLLCCALCCPCFHKERKANSHEVLPKESNSVHQVSSF 191

Query: 190 VMSSAPEKIPPSPLRVPPSP--------RYSPSPKLKRLGSVHLNMSQVVRATRDFSPSL 249
            MS + EKIP SP R PPSP        RY+ SP+  RLG ++L MSQ+  AT +F+ S 
Sbjct: 192 EMSPSSEKIPQSPFRAPPSPSRVPQSPSRYAMSPRPSRLGPLNLTMSQINTATGNFADSH 251

Query: 250 RIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVELLAKIDHRSLVKLLGY 309
           +IGEGGFG V+K  L+DG +VA+KRAKK E FEN RT+F SEV+LL+KI HR+LVKLLGY
Sbjct: 252 QIGEGGFGVVFKGVLDDGQVVAIKRAKK-EHFENLRTEFKSEVDLLSKIGHRNLVKLLGY 311

Query: 310 VDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAHGLTYLHLYAEKQIIH 369
           VD G+ER+IITEYV NGTLR+HLDG  G  L+FNQRLEI ID+ HGLTYLH YAE+QIIH
Sbjct: 312 VDKGDERLIITEYVRNGTLRDHLDGARGTKLNFNQRLEIVIDVCHGLTYLHSYAERQIIH 371

Query: 370 RDVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTVGYLDPEYMKTYQLTTK 429
           RD+KS+NILLT+SMRAKVADFGFAR G   + QTHI TQVKGTVGYLDPEYMKTY LT K
Sbjct: 372 RDIKSSNILLTDSMRAKVADFGFARGGPTDSNQTHILTQVKGTVGYLDPEYMKTYHLTAK 431

Query: 430 SDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKILETLDPLLDETVDVEI 489
           SDVYSFGILLVEILTGRRP+EAKR P+ER+T+RWAF KY++ ++ E +DP   E VD +I
Sbjct: 432 SDVYSFGILLVEILTGRRPVEAKRLPDERITVRWAFDKYNEGRVFELVDPNARERVDEKI 491

Query: 490 VVKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKD 520
           + KMF LA QCAAP + +RPDM+ VG+QLWAIR+ Y+ +  +
Sbjct: 492 LRKMFSLAFQCAAPTKKERPDMEAVGKQLWAIRSSYLRRSME 510

BLAST of MS002403 vs. TAIR 10
Match: AT5G58940.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 )

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-94
Identity = 165/306 (53.92%), Postives = 224/306 (73.20%), Query Frame = 0

Query: 208 SPSPKLKRLGSVHLNMSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQE 267
           S S K    G V  +  ++ RAT +FS   +IGEGGFGTV+K +L+DG IVA+KRA+K  
Sbjct: 122 SSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDDGTIVAIKRARKNN 181

Query: 268 IFENSRTDFSSEVELLAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKV 327
             ++   +F +E+  L+KI+H +LVKL G+++HG+E++I+ EYV NG LREHLDG+ G  
Sbjct: 182 YGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGLRGNR 241

Query: 328 LDFNQRLEIAIDIAHGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLG 387
           L+  +RLEIAID+AH LTYLH Y +  IIHRD+K++NIL+T  +RAKVADFGFARL +  
Sbjct: 242 LEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARLVSED 301

Query: 388 TEQTHISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERV 447
              THISTQVKG+ GY+DP+Y++T+QLT KSDVYSFG+LLVEILTGRRP+E KRP ++R+
Sbjct: 302 LGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPRKDRL 361

Query: 448 TIRWAFTKYSDDKILETLDPLLDET-VDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQL 507
           T++WA  +  DD+ +  +DP L      +E+  KM  LA +C  P RA RP MK + E+L
Sbjct: 362 TVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGIAEKL 421

Query: 508 WAIRAD 513
           WAIR +
Sbjct: 422 WAIRRE 427

BLAST of MS002403 vs. TAIR 10
Match: AT4G00330.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 )

HSP 1 Score: 335.5 bits (859), Expect = 7.7e-92
Identity = 166/293 (56.66%), Postives = 211/293 (72.01%), Query Frame = 0

Query: 225 QVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKK--QEIFENSRTDFSSEVEL 284
           ++  AT++FSPS RIG+GGFGTVYK +L DG   AVKRAKK   +  + +  +F SE++ 
Sbjct: 111 EIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQGADAEFMSEIQT 170

Query: 285 LAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFNQRLEIAIDIAH 344
           LA++ H SLVK  G+V H +E+I++ EYV NGTLR+HLD   GK LD   RL+IA D+AH
Sbjct: 171 LAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMATRLDIATDVAH 230

Query: 345 GLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLG-TLGTEQTHISTQVKGTV 404
            +TYLH+Y +  IIHRD+KS+NILLTE+ RAKVADFGFARL     +  TH+STQVKGT 
Sbjct: 231 AITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGATHVSTQVKGTA 290

Query: 405 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKI 464
           GYLDPEY+ TYQLT KSDVYSFG+LLVE+LTGRRP+E  R  +ER+TIRWA  K++    
Sbjct: 291 GYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIRWAIKKFTSGDT 350

Query: 465 LETLDPLLDETVDVEIVV-KMFELAIQCAAPVRADRPDMKLVGEQLWAIRADY 514
           +  LDP L++     + + K+ E+A QC AP R  RP MK   E LW IR DY
Sbjct: 351 ISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGIRKDY 403

BLAST of MS002403 vs. TAIR 10
Match: AT5G54380.1 (protein kinase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.7e-62
Identity = 135/296 (45.61%), Postives = 174/296 (58.78%), Query Frame = 0

Query: 216 LGSVHLN----MSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFEN 275
           L S HL       +++ AT  F  S  +G GGFG VYK  LEDG  VAVKR   +   E 
Sbjct: 489 LASTHLGRCFMFQEIMDATNKFDESSLLGVGGFGRVYKGTLEDGTKVAVKRGNPRS--EQ 548

Query: 276 SRTDFSSEVELLAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKVLDFN 335
              +F +E+E+L+K+ HR LV L+GY D  +E I++ EY+ NG LR HL G     L + 
Sbjct: 549 GMAEFRTEIEMLSKLRHRHLVSLIGYCDERSEMILVYEYMANGPLRSHLYGADLPPLSWK 608

Query: 336 QRLEIAIDIAHGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLGTEQT 395
           QRLEI I  A GL YLH  A + IIHRDVK+TNILL E++ AKVADFG ++ G    +QT
Sbjct: 609 QRLEICIGAARGLHYLHTGASQSIIHRDVKTTNILLDENLVAKVADFGLSKTGP-SLDQT 668

Query: 396 HISTQVKGTVGYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRW 455
           H+ST VKG+ GYLDPEY +  QLT KSDVYSFG++L+E+L  R  L    P E+     W
Sbjct: 669 HVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLMEVLCCRPALNPVLPREQVNIAEW 728

Query: 456 AFTKYSDDKILETLDPLLDETVDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQLW 508
           A        + + +D  L   V+   + K  E A +C A    DRP M   G+ LW
Sbjct: 729 AMAWQKKGLLDQIMDSNLTGKVNPASLKKFGETAEKCLAEYGVDRPSM---GDVLW 778

BLAST of MS002403 vs. TAIR 10
Match: AT2G39360.1 (Protein kinase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 4.1e-61
Identity = 132/299 (44.15%), Postives = 178/299 (59.53%), Query Frame = 0

Query: 223 MSQVVRATRDFSPSLRIGEGGFGTVYKAQLEDGHIVAVKRAKKQEIFENSRTDFSSEVEL 282
           ++ +  AT DF  SL IG GGFG VYK  L D   VAVKR   Q        +F +EVE+
Sbjct: 477 LALIKEATDDFDESLVIGVGGFGKVYKGVLRDKTEVAVKRGAPQS--RQGLAEFKTEVEM 536

Query: 283 LAKIDHRSLVKLLGYVDHGNERIIITEYVGNGTLREHLDGVHGKV-LDFNQRLEIAIDIA 342
           L +  HR LV L+GY D  +E II+ EY+  GTL++HL  +  K  L + QRLEI +  A
Sbjct: 537 LTQFRHRHLVSLIGYCDENSEMIIVYEYMEKGTLKDHLYDLDDKPRLSWRQRLEICVGAA 596

Query: 343 HGLTYLHLYAEKQIIHRDVKSTNILLTESMRAKVADFGFARLGTLGTEQTHISTQVKGTV 402
            GL YLH  + + IIHRDVKS NILL ++  AKVADFG ++ G    +QTH+ST VKG+ 
Sbjct: 597 RGLHYLHTGSTRAIIHRDVKSANILLDDNFMAKVADFGLSKTGP-DLDQTHVSTAVKGSF 656

Query: 403 GYLDPEYMKTYQLTTKSDVYSFGILLVEILTGRRPLEAKRPPEERVTIRWAFTKYSDDKI 462
           GYLDPEY+   QLT KSDVYSFG++++E++ GR  ++   P E+   I WA       K+
Sbjct: 657 GYLDPEYLTRQQLTEKSDVYSFGVVMLEVVCGRPVIDPSLPREKVNLIEWAMKLVKKGKL 716

Query: 463 LETLDPLLDETVDVEIVVKMFELAIQCAAPVRADRPDMKLVGEQLWAIRADYISQKKDK 521
            + +DP L   V +E V K  E+  +C +    +RP M   G+ LW +      Q KD+
Sbjct: 717 EDIIDPFLVGKVKLEEVKKYCEVTEKCLSQNGIERPAM---GDLLWNLEFMLQVQAKDE 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135089.13.2e-29799.81calmodulin-binding receptor-like cytoplasmic kinase 3 [Momordica charantia][more]
XP_022960815.19.0e-25284.38calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita moschata] >XP_0... [more]
KAG6589818.11.3e-25084.00Calmodulin-binding receptor-like cytoplasmic kinase 3, partial [Cucurbita argyro... [more]
XP_038879737.16.5e-25084.95calmodulin-binding receptor-like cytoplasmic kinase 3 [Benincasa hispida][more]
XP_022987686.15.5e-24984.00calmodulin-binding receptor-like cytoplasmic kinase 3 [Cucurbita maxima] >XP_022... [more]
Match NameE-valueIdentityDescription
Q9ASQ55.5e-14352.87Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX... [more]
Q9FIL72.3e-9353.92Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX... [more]
Q8VZJ91.1e-9056.66Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX... [more]
Q9LK352.4e-6145.61Receptor-like protein kinase THESEUS 1 OS=Arabidopsis thaliana OX=3702 GN=THE1 P... [more]
O806235.8e-6044.15Probable receptor-like protein kinase At2g39360 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C0H61.5e-29799.81calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Momordica charantia OX=... [more]
A0A6J1H8N34.4e-25284.38calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita moschata OX=3... [more]
A0A6J1JK632.6e-24984.00calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucurbita maxima OX=366... [more]
A0A5D3C3663.3e-24484.03Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucumis melo var. makuw... [more]
A0A5A7U9155.7e-24483.84Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT2G11520.13.9e-14452.87calmodulin-binding receptor-like cytoplasmic kinase 3 [more]
AT5G58940.11.7e-9453.92calmodulin-binding receptor-like cytoplasmic kinase 1 [more]
AT4G00330.17.7e-9256.66calmodulin-binding receptor-like cytoplasmic kinase 2 [more]
AT5G54380.11.7e-6245.61protein kinase family protein [more]
AT2G39360.14.1e-6144.15Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 233..508
e-value: 2.0E-37
score: 140.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 233..508
score: 38.562511
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 311..522
e-value: 6.4E-56
score: 190.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 208..310
e-value: 2.2E-27
score: 97.0
NoneNo IPR availablePIRSRPIRSR000605-50PIRSR000605-50coord: 220..439
e-value: 3.0E-17
score: 60.4
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 239..441
e-value: 2.7E-28
score: 97.1
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 239..440
e-value: 4.8E-14
score: 49.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..214
NoneNo IPR availablePANTHERPTHR27003OS07G0166700 PROTEINcoord: 95..514
NoneNo IPR availablePANTHERPTHR27003:SF274NODULATION RECEPTOR KINASE-LIKE PROTEINcoord: 95..514
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 239..506
e-value: 2.37425E-90
score: 276.076
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 238..505
e-value: 4.6E-49
score: 167.0
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 355..367
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 239..265
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 215..509

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002403.1MS002403.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity